JP2008289502A - ビフィドバクテリウム属細菌 - Google Patents
ビフィドバクテリウム属細菌 Download PDFInfo
- Publication number
- JP2008289502A JP2008289502A JP2008215753A JP2008215753A JP2008289502A JP 2008289502 A JP2008289502 A JP 2008289502A JP 2008215753 A JP2008215753 A JP 2008215753A JP 2008215753 A JP2008215753 A JP 2008215753A JP 2008289502 A JP2008289502 A JP 2008289502A
- Authority
- JP
- Japan
- Prior art keywords
- linoleic acid
- bifidobacterium
- conjugated
- acid
- milk
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000186000 Bifidobacterium Species 0.000 title claims abstract description 58
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- JBYXPOFIGCOSSB-GOJKSUSPSA-N 9-cis,11-trans-octadecadienoic acid Chemical compound CCCCCC\C=C\C=C/CCCCCCCC(O)=O JBYXPOFIGCOSSB-GOJKSUSPSA-N 0.000 claims abstract description 47
- 229940108924 conjugated linoleic acid Drugs 0.000 claims abstract description 42
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 claims abstract description 25
- 235000020778 linoleic acid Nutrition 0.000 claims abstract description 25
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Abstract
【解決手段】 リノール酸をcis-9,trans-11型の共役リノール酸に変換する能力を有するビフィドバクテリウム属細菌であって、次の (a) 及び (b) 工程によって、培地中に少なくとも0.5mg/5mlの共役リノール酸を産生する能力を有することを特徴とするもの。
(a) リノール酸−BSA−複合体を含むMRS培地にて少なくとも24時間前培養する工程、
(b) リノール酸−BSA−複合体を含む乳培地に、工程(a) で得られたビフィドバクテリウム属細菌を3.8%接種し、144時間振盪培養する工程、
【選択図】 なし
Description
(a) リノール酸−BSA−複合体を含むMRS培地にて少なくとも24時間前培養する工程、
(b) リノール酸−BSA−複合体を含む乳培地に、工程(a) で得られたビフィドバクテリウム属細菌を3.8%接種し、144時間振盪培養する工程、
このように優れた産生能を有する株は、これを用いてリノール酸を含む食品素材(例えば、乳原料)を発酵した場合に、発酵後の発酵食品(例えば、発酵乳)中に、生理効果を期待できる程度の共役リノール酸を産生させることができるので、食品製造の作業性等において特に適したものである。
(b) 不飽和脂肪酸をビフィドバクテリウム属細菌の洗浄菌体で共役化する方法
(c) ビフィドバクテリウム属細菌菌末を用いて不飽和脂肪酸を共役化する方法
(d) ビフィドバクテリウム属細菌死菌体を用いて不飽和脂肪酸を共役化する方法
(e) ビフィドバクテリウム属細菌菌体抽出液を用いて不飽和脂肪酸を共役化する方法
(a) ビフィドバクテリウム属細菌を増殖培地や発酵乳中で培養して発酵生産の形で不飽和脂肪酸を共役化する方法:
増殖培地あるいは発酵乳中で共役化を行う際は、基本操作は一般的に行われている手法で行えば良いが、スターターとしては以下のものを用いるのが好ましい。
処理法(a) のスターター調製時と同様の培養条件によって培養した菌体を生理食塩水にて洗浄したものを回収し、緩衝液に懸濁させた。この菌体を懸濁させる緩衝液および洗浄菌体反応は適度なpHを維持できる条件の水溶液を用いて行う。例えば0.1〜1.0Mのリン酸緩衝液が好ましく、pHは5.0〜7.5であるが、好ましくは6.0〜7.0である。菌体懸濁液の菌体濃度は、湿重で0.025〜0.25%であり、好ましくは0.025〜0.1%である。原料は、添加時の原料を牛血清アルブミン(BSA)との混合液としており原料濃度は反応溶液中に0.1〜4.0%程度添加することが好ましく、特に0.3〜1.0%が好ましい。
ビフィドバクテリウム属細菌菌末は、例えば処理法(a) のスターター調製時の培養条件と同様の方法にて得たビフィドバクテリウム属細菌菌体を乾燥処理により粉末化することにより得ることが出来る。乾燥処理方法としては凍結乾燥、噴霧乾燥などを利用することができる。なお、菌末調製後の変換反応は、処理法(b) の洗浄菌体反応の反応条件と同様に行えば良い。
ビフィドバクテリウム属細菌菌体は、例えば処理法(a) のスターター調製時の培養条件と同様の方法にて得たビフィドバクテリウム属細菌菌体の細胞壁を破壊することにより得ることが出来る。細胞壁破壊は、細胞壁破壊酵素で処理する方法や、菌体を溶媒に懸濁させて低浸透圧で処理する方法、凍結融解する方法、高圧処理する方法、破砕処理する方法、加熱処理する方法などを利用することができる。細胞壁破壊液調整後の変換反応は、処理法(b) の洗浄菌体反応の反応条件と同様に行えばよい。
ビフィドバクテリウム属細菌菌体抽出液としては、例えば処理法(b) 〜(d) と同様の方法にて得たビフィドバクテリウム属細菌洗浄菌体、菌体末、死菌体などを適切な溶媒で抽出し、遠心分離などの方法により残漬を除去して得ることが出来る。なお、菌体抽出液調製後の反応は、処理法(b) の洗浄菌体反応の反応条件と同様に行えばよい。
100mMリン酸緩衝液(pH 6.5)1mL中にリノール酸50mgとBSA10mgを溶解し、あらかじめリノール酸−BSA−複合体溶液を調製した。このリノール酸−BSA−複合体溶液200μLをMRS(LACTOBACILLI MRS BROTH、DIFCO社製)培地15mLに添加後、bifidobacterium属15菌株をそれぞれ接種し35℃、120rpm、48時間振盪培養し、ビフィドバクテリウム属細菌培養液を調製した。
100mMリン酸緩衝液(pH 6.5)1mL中にリノール酸50mgとBSA10mgを溶解し、あらかじめリノール酸−BSA−複合体溶液を調製した。
脱脂粉乳を10%、グルコースを1%、大豆ペプチドを 0.1%含む培地に、基質としてリノール酸を0.1%または1.0%添加して150kg/cm2 の均質化処理を行った後、オートクレーブで115℃、10分間の滅菌処理を行い発酵食品調製用培地を作製した。
Claims (1)
- リノール酸をcis-9,trans-11型の共役リノール酸に変換する能力を有するビフィドバクテリウム属細菌であって、次の (a) 及び (b) 工程によって、培地中に少なくとも0.5mg/5mlの共役リノール酸を産生する能力を有することを特徴とするビフィドバクテリウム属細菌。
(a) リノール酸−BSA−複合体を含むMRS培地にて少なくとも24時間前培養する工程、
(b) リノール酸−BSA−複合体を含む乳培地に、工程(a) で得られたビフィドバクテリウム属細菌を3.8%接種し、144時間振盪培養する工程、
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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WO1999029886A1 (en) * | 1997-12-05 | 1999-06-17 | Bjoerck Lennart | Formation of conjugated unsaturated fatty acids |
WO2000064854A1 (fr) * | 1999-04-27 | 2000-11-02 | Kabushiki Kaisha Yakult Honsha | Esters d'acides gras conjugues |
JP2002017381A (ja) * | 2000-07-07 | 2002-01-22 | Meiji Seika Kaisha Ltd | 微生物を用いた共役リノール酸の製造方法 |
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WO1999029886A1 (en) * | 1997-12-05 | 1999-06-17 | Bjoerck Lennart | Formation of conjugated unsaturated fatty acids |
WO2000064854A1 (fr) * | 1999-04-27 | 2000-11-02 | Kabushiki Kaisha Yakult Honsha | Esters d'acides gras conjugues |
EP1174416A1 (en) * | 1999-04-27 | 2002-01-23 | Kabushiki Kaisha Yakult Honsha | Conjugated fatty acid esters |
JP2002017381A (ja) * | 2000-07-07 | 2002-01-22 | Meiji Seika Kaisha Ltd | 微生物を用いた共役リノール酸の製造方法 |
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