JP2008260740A - Electrolyzed water composition having bactericidal action and hemostatic action - Google Patents
Electrolyzed water composition having bactericidal action and hemostatic action Download PDFInfo
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本発明は、殺菌或いは消毒に用いられる電解水組成物に関するものである。 The present invention relates to an electrolyzed water composition used for sterilization or disinfection.
有隔膜の電気分解槽でナトリウムイオンや塩素イオンを含有する水を電気分解すると、陽極側には酸性電解水が生成され、陰極側にはアルカリ性電解水が生成される。特に、酸性電解水には、殺菌作用があり、殺菌剤として用いられる。一方、アルカリ性電解水には、脱脂作用があり、タンパク質や油脂による汚れ等を落とす脱脂剤として用いられる。こうした電解水の用途を拡大すべく、種々の技術が既に提案されている。 When water containing sodium ions or chlorine ions is electrolyzed in the electrolysis tank of the diaphragm, acidic electrolyzed water is generated on the anode side and alkaline electrolyzed water is generated on the cathode side. In particular, acidic electrolyzed water has a bactericidal action and is used as a bactericidal agent. On the other hand, alkaline electrolyzed water has a degreasing action and is used as a degreasing agent that removes dirt and the like due to proteins and oils. Various techniques have already been proposed to expand the use of such electrolyzed water.
例えば、特許文献１には、ｐＨが２．７以下の酸性電解水に結合剤を加えてその粘度を高くした電解水組成物が記載されており、この電解水組成物は皮膚ひきしめ化粧料や殺菌剤として使用できることが記載されている。 For example, Patent Document 1 describes an electrolyzed water composition having a viscosity increased by adding a binder to acidic electrolyzed water having a pH of 2.7 or less. It is described that it can be used as a disinfectant.
しかし特許文献１に記載されている電解水組成物は、ｐＨが２．７以下の強酸性電解水を用いているため、敏感肌の人や皮膚が弱い局部や創傷部位に対しては、刺激が強く使用に適さないという問題があった。また、電解水組成物が強酸性になると、歯に対して脱灰作用や侵蝕作用が生じるため、特に口腔内での使用には適していない。しかも酸臭が強くなると、使用者に不快感を与えてしまうため、この点でも口腔内での使用には適していない。 However, since the electrolyzed water composition described in Patent Document 1 uses strongly acidic electrolyzed water having a pH of 2.7 or less, it is irritating to people with sensitive skin, weak local areas or wound sites. However, there was a problem that it was not suitable for use. In addition, when the electrolyzed water composition becomes strongly acidic, a decalcification action and an erosion action occur on the teeth, and therefore, it is not particularly suitable for use in the oral cavity. In addition, when the acid odor becomes strong, the user feels uncomfortable, and this point is not suitable for use in the oral cavity.
ところで、無隔膜の電気分解槽で水を電気分解して得られる電解水の用途を拡大するための技術についても既に提案されている。例えば、特許文献２には、塩化ナトリウムを含有しない水に塩酸を添加した塩酸添加水を無隔膜電解槽で電気分解し、得られたｐＨが５．６〜６．８の範囲である中性付近の電解水を皮膚疾患治療剤として使用することが記載されている。この方法によれば、実質的にＮａイオンを含有しないため残留する食塩に起因する痛み等の苦痛を低減できる旨記載されている。しかし特許文献２では、原料として劇物に指定されている塩酸を用いているため、製造設備等を腐食する恐れがあり、また安全性にも問題がある。 By the way, the technique for expanding the use of the electrolyzed water obtained by electrolyzing water in the electrolysis tank of a non-membrane is already proposed. For example, Patent Document 2 discloses a neutral solution in which hydrochloric acid-added water obtained by adding hydrochloric acid to water not containing sodium chloride is electrolyzed in a diaphragm electrolyzer, and the resulting pH is in the range of 5.6 to 6.8. The use of nearby electrolyzed water as a skin disease treatment is described. According to this method, it is described that pain such as pain caused by residual salt can be reduced because it does not substantially contain Na ions. However, in Patent Document 2, since hydrochloric acid designated as a deleterious substance is used as a raw material, there is a risk of corroding manufacturing equipment and there is a problem in safety.
一方、本出願人らは、殺菌性が高い次亜塩素酸溶液をｐＨが中性付近の安定した状態で得ることができ、且つ残存ＮａＣｌ等の含有量を大幅に低減できる方法として、殺菌性を有する中性付近の電解水を製造する方法および装置を先に提案している。例えば、特許文献３には、金属塩化物（例えば、塩化ナトリウムなど）の水溶液を無隔膜電解槽で電気分解し、生成した電解液を更に隔膜電解槽の陽極側に導入すると共に、該隔膜電解槽の陰極側には金属塩化物水溶液（例えば、塩化ナトリウム水溶液など）を導入し、これを電気分解することを提案している。 On the other hand, the present applicants can obtain a hypochlorous acid solution having a high bactericidal property in a stable state near a neutral pH, and a method that can significantly reduce the content of residual NaCl and the like. A method and apparatus for producing near-neutral electrolyzed water having the above has been proposed previously. For example, in Patent Document 3, an aqueous solution of a metal chloride (for example, sodium chloride) is electrolyzed in a diaphragm electrolytic cell, and the generated electrolytic solution is further introduced into the anode side of the diaphragm electrolytic cell, and the diaphragm electrolysis is performed. It has been proposed to introduce a metal chloride aqueous solution (for example, a sodium chloride aqueous solution) into the cathode side of the cell and electrolyze it.
また、特許文献４には、塩化ナトリウムおよび／または塩化カリウム水溶液を隔膜電解法と無隔膜電解法を組み合わせて電解水を製造する技術を提案している。
本発明は、中性付近の電解水の用途を更に拡大、発展させることを目指して成されたものであり、具体的には、殺菌或いは消毒する際に使用することができ、しかも止血作用も有し、この殺菌作用および止血作用の持続性が長時間にわたって発揮される電解水組成物を提供することにある。また、本発明の他の目的は、本発明の電解水組成物を口腔内で使用した場合には、歯を脱灰することなく口腔内を殺菌或いは消毒することができ、しかも口腔内に発生した出血を止血でき、この殺菌作用および止血作用の持続性が長時間にわたって発揮される電解水組成物を提供することにある。 The present invention has been made with the aim of further expanding and developing the use of electrolyzed water in the vicinity of neutrality. Specifically, it can be used for sterilization or disinfection, and also has a hemostatic action. It is an object of the present invention to provide an electrolyzed water composition that can maintain the bactericidal action and the hemostatic action for a long time. Another object of the present invention is that when the electrolyzed water composition of the present invention is used in the oral cavity, the oral cavity can be sterilized or disinfected without demineralizing the teeth, and is also generated in the oral cavity. It is an object of the present invention to provide an electrolyzed water composition that can stop bleeding that has occurred and that exhibits the bactericidal action and the persistence of the hemostatic action over a long period of time.
上記課題を解決することのできた本発明に係る殺菌作用および止血作用を有する電解水組成物とは、塩化ナトリウム含有水溶液を電気分解して得られる電解水と、植物性多糖類とを含有する電解水組成物であって、前記電解水組成物中における前記植物性多糖類の比率が０．１０〜２．５％（質量％の意味。以下同じ。）であり、有効塩素濃度が１５ｐｐｍ（質量基準。以下同じ。）以上、且つｐＨが５．０〜８．５である点に要旨を有する。 The electrolyzed water composition having a bactericidal action and a hemostatic action according to the present invention that has solved the above-mentioned problems is an electrolysis containing electrolyzed water obtained by electrolyzing a sodium chloride-containing aqueous solution and a plant polysaccharide. It is a water composition, Comprising: The ratio of the said vegetable polysaccharide in the said electrolyzed water composition is 0.10-2.5% (meaning of mass%, hereafter the same), and effective chlorine concentration is 15 ppm (mass). The same applies to the following.) Further, the point is that the pH is 5.0 to 8.5.
前記植物性多糖類としては、例えば、寒天、アルギン酸、カラギーナン、キサンタンガム、グアーガムおよびローカストビーンガムよりなる群から選択される少なくとも１種を含有することができる、前記電解水としては、ｐＨが５．０〜８．５の電解水を用いるのがよい。 The plant polysaccharide can contain, for example, at least one selected from the group consisting of agar, alginic acid, carrageenan, xanthan gum, guar gum and locust bean gum. The electrolyzed water has a pH of 5. It is preferable to use 0 to 8.5 electrolyzed water.
本発明によれば、塩化ナトリウム含有水溶液を電気分解して得られる電解水（例えば、ｐＨ５．０〜８．５の電解水）と、適量の植物性多糖類を配合した電解水組成物のｐＨと、該電解水組成物に含まれる有効塩素濃度を適切な範囲に調整しているため、患部を殺菌或いは消毒でき、しかも出血を止血する作用も有している。特に、本発明の電解水組成物は、原料として用いる電解水よりも粘度が高いため、形状保持性に優れており、殺菌力の持続性が高められるほか、出血部分（患部）に長時間留まって殺菌作用や止血作用を発揮する。こうした本発明の電解水組成物を口腔内で使用しても、本発明の電解水組成物は歯を脱灰する作用を有していないため、歯を脱灰することなく、口腔内を殺菌或いは消毒でき、口腔内に発生した出血を止血できる。 According to the present invention, the pH of an electrolyzed water composition comprising electrolyzed water obtained by electrolyzing a sodium chloride-containing aqueous solution (for example, electrolyzed water having a pH of 5.0 to 8.5) and an appropriate amount of plant polysaccharides. In addition, since the effective chlorine concentration contained in the electrolyzed water composition is adjusted to an appropriate range, the affected area can be sterilized or disinfected, and it also has the effect of stopping bleeding. In particular, since the electrolyzed water composition of the present invention has a higher viscosity than the electrolyzed water used as a raw material, it has excellent shape retention, enhances the sterilizing power, and stays in the bleeding part (affected part) for a long time. It demonstrates bactericidal and hemostatic effects. Even if such an electrolyzed water composition of the present invention is used in the oral cavity, the electrolyzed water composition of the present invention does not have a function of demineralizing the teeth, so the oral cavity is sterilized without demineralizing the teeth. Or it can disinfect and can stop bleeding that has occurred in the oral cavity.
本発明の電解水組成物は、患部の殺菌或いは消毒に用いることができ、しかも患部に長時間留まって出血を確実に止血できる電解水組成物を提供すべく検討を重ねてきた。その結果、塩化ナトリウム含有水溶液を電気分解して得られる電解水（例えば、ｐＨ５．０〜８．５の電解水）と、植物性多糖類を配合されていると共に、電解水組成物全体に占める前記植物性多糖類量が０．１０〜２．５％で、且つ電解水組成物のｐＨが５．０〜８．５、有効塩素濃度が１５ｐｐｍ以上であればよい。 The electrolyzed water composition of the present invention has been studied to provide an electrolyzed water composition that can be used for sterilization or disinfection of an affected area, and that can remain in the affected area for a long time and can stop bleeding reliably. As a result, electrolyzed water obtained by electrolyzing a sodium chloride-containing aqueous solution (for example, electrolyzed water having a pH of 5.0 to 8.5) and a vegetable polysaccharide are blended and occupy the entire electrolyzed water composition. The plant polysaccharide content may be 0.10 to 2.5%, the pH of the electrolyzed water composition may be 5.0 to 8.5, and the effective chlorine concentration may be 15 ppm or more.
電解水に所定量の植物性多糖類を配合することで、電解水組成物の形態をゲル状またはジェル状にできるため、該電解水組成物を患部に塗布しても電解水組成物の形状を長時間保持でき（以下、形状保持性ということがある）、患部に殺菌作用および止血作用を長時間にわたって発揮させることができる。即ち、電解水を含有するが、植物性多糖類を含有しない電解水組成物では、流動性が高いため、患部に滞留する時間が非常に短く、患部を完治させるには当該電解水組成物を複数回塗布しなければならない。これに対し、本発明のように、植物性多糖類を所定量配合して電解水組成物の形態をゲル状またはジェル状にすることで、当該電解水組成物を患部に長時間滞留させることができ、殺菌作用や止血作用を長時間にわたって効果的に発揮させることができる。また、患部に長時間滞留することで、新たな細菌の混入による感染も防止できる。 By adding a predetermined amount of vegetable polysaccharide to the electrolyzed water, the form of the electrolyzed water composition can be made into a gel or gel. Therefore, even if the electrolyzed water composition is applied to the affected area, the shape of the electrolyzed water composition Can be maintained for a long time (hereinafter sometimes referred to as shape retention), and the affected part can exhibit a bactericidal action and a hemostatic action for a long time. That is, in an electrolyzed water composition containing electrolyzed water but not containing plant polysaccharides, the fluidity is high, so that the residence time in the affected area is very short, and the electrolyzed water composition is used to completely cure the affected area. Must be applied multiple times. On the other hand, like the present invention, a predetermined amount of vegetable polysaccharide is blended and the form of the electrolyzed water composition is made into a gel or gel, thereby allowing the electrolyzed water composition to stay in the affected area for a long time. It is possible to effectively exert a bactericidal action and a hemostatic action over a long period of time. In addition, by staying in the affected area for a long time, infection due to contamination with new bacteria can also be prevented.
また、電解水組成物に含まれる殺菌力のある有効塩素は、タンパク質と接触すると殺菌作用が消失するが、電解水組成物の形態をゲル状またはジェル状とすることで、電解水組成物の流動性が低下して、有効塩素がタンパク質と接触するのを妨げることができる。その結果、殺菌作用や止血作用を持続的に発揮させることができる。 In addition, effective bactericidal chlorine contained in the electrolyzed water composition loses its bactericidal action when it comes into contact with protein. However, by making the electrolyzed water composition into a gel or gel form, The fluidity can be reduced to prevent the available chlorine from contacting the protein. As a result, a bactericidal action and a hemostatic action can be exhibited continuously.
上記のように、本発明の電解水組成物は、形状保持性に優れており、例えば、ゲル状（固体状）またはジェル状（半液体、半固体状）として存在している。本発明において、ゲル状とは、ゲル強度が５０ｇ／ｃｍ2以上の状態を意味する。ゲル強度の上限は特に限定されないが、ゲル強度が大きくなり過ぎると、患部に塗布し難くなるので、上限は１０００ｇ／ｃｍ2程度とするのがよい。 As described above, the electrolyzed water composition of the present invention is excellent in shape retention, and exists, for example, as a gel (solid) or gel (semi-liquid, semi-solid). In the present invention, the gel form means a state where the gel strength is 50 g / cm 2 or more. The upper limit of the gel strength is not particularly limited, but if the gel strength becomes too high, it becomes difficult to apply to the affected area, so the upper limit is preferably about 1000 g / cm 2 .
ゲル強度は、例えば、日寒水式の方法で測定できる。即ち、植物性多糖類を含む水溶液を調製し、２０℃で１５時間放置し、凝固させたゲルについて、その表面１ｃｍ2当たり２０秒間耐え得る最大重量（ｇ）をゲル強度とする。 The gel strength can be measured by, for example, a Nissho water method. That is, an aqueous solution containing vegetable polysaccharides is prepared, and allowed to stand at 20 ° C. for 15 hours to solidify the gel, and the maximum weight (g) that can withstand 20 seconds per 1 cm 2 of the surface is defined as the gel strength.
本発明において、ジェル状とは、ゲル状ほどの強度はないが、無応力下ではほとんど流動せずその形状を保持できる半液体、半固体状を意味し、粘度が１Ｐａ・ｓ以上である半液体、半固体状を指す。粘度の上限は特に限定されないが、粘度が大きくなり過ぎると、患部に塗布し難くなるので、上限は１０００Ｐａ・ｓ程度とするのがよい。なお、ジェルの粘度は、例えば、Ｂ型回転粘度計を用いて測定できる。 In the present invention, the gel form means a semi-liquid or semi-solid form that does not flow as much as a gel, but hardly flows under no stress, and can maintain its shape, and has a viscosity of 1 Pa · s or more. Liquid, semi-solid state. The upper limit of the viscosity is not particularly limited, but if the viscosity becomes too high, it becomes difficult to apply to the affected area, so the upper limit is preferably about 1000 Pa · s. The gel viscosity can be measured using, for example, a B-type rotational viscometer.
本発明では、上記電解水と植物性多糖類を配合することが重要であるが、電解水組成物全体に占める前記植物性多糖類量（乾燥質量）は０．１０〜２．５％に調整する。植物性多糖類を配合することで、電解水組成物の粘度を高めることができ、形状保持性が良くなり、本発明の電解水組成物を患部に長時間留めることができる。その結果、後述するように、止血作用が長時間にわたって発揮され、患部の出血を確実に止血できる。 In the present invention, it is important to blend the electrolyzed water and the vegetable polysaccharide, but the amount of the vegetable polysaccharide (dry mass) in the entire electrolyzed water composition is adjusted to 0.10 to 2.5%. To do. By blending the plant polysaccharide, the viscosity of the electrolyzed water composition can be increased, the shape retention is improved, and the electrolyzed water composition of the present invention can be kept on the affected part for a long time. As a result, as will be described later, the hemostatic action is exhibited over a long period of time, and bleeding in the affected area can be reliably stopped.
上記電解水組成物に占める上記植物性多糖類の配合量（乾燥質量）が、０．１０％を下回ると、電解水組成物の粘度が低くなり過ぎるため、ジェル状にならず、流動性が高くなり過ぎる。そのため電解水組成物の形状保持性が悪くなり、患部に長時間留まらせることができない。また、本発明の電解水組成物をチューブ等に充填して、押し出しつつ患部に供給しようとしても、粘度が低過ぎるため、液だれ等を起こし、作業性が悪くなる。従って配合量は０．１０％以上とし、好ましくは０．２％以上である。しかし配合量が２．５％を超えると、ゲル強度が大きくなり過ぎて患部へ塗布し難くなる。従って配合量は２．５％以下とし、好ましくは２％以下、より好ましくは１．８％以下、特に１．０％以下とする。 When the blending amount (dry mass) of the vegetable polysaccharide in the electrolyzed water composition is less than 0.10%, the viscosity of the electrolyzed water composition becomes too low, so that it does not become a gel and has fluidity. Too high. For this reason, the shape retention of the electrolyzed water composition is deteriorated, and the electrolyzed water composition cannot remain in the affected area for a long time. Moreover, even if it fills a tube etc. with the electrolyzed water composition of this invention, and it tries to supply it to an affected part while pushing out, since a viscosity is too low, a liquid dripping etc. will be caused and workability | operativity will worsen. Accordingly, the blending amount is 0.10% or more, preferably 0.2% or more. However, if the blending amount exceeds 2.5%, the gel strength becomes too high and it becomes difficult to apply to the affected area. Accordingly, the blending amount is 2.5% or less, preferably 2% or less, more preferably 1.8% or less, and particularly 1.0% or less.
本発明に用いられる植物性多糖類としては、植物由来の多糖類または多糖類を含有する物質であり、本発明の電解水組成物による殺菌作用や止血作用等を阻害せずに増粘作用やゲル化作用を有するものが挙げられる。植物性多糖類の種類は特に限定されないが、例えば、寒天、アルギン酸、カラギーナン、キサンタンガム、グアーガムおよびローカストビーンガム、ペクチンなどを挙げることができる。但し、ペクチンは、主として強酸性領域でその増粘作用が発揮されるため、本発明のようにｐＨが５．０〜８．５の範囲では粘度向上作用は有効に発揮され難いが、粘度が低いジェルタイプの電解水組成物を製造する際には用いることができる。 The plant polysaccharide used in the present invention is a plant-derived polysaccharide or a substance containing a polysaccharide, and does not inhibit the bactericidal action, hemostatic action, etc. of the electrolyzed water composition of the present invention. The thing which has a gelatinization effect | action is mentioned. The type of plant polysaccharide is not particularly limited, and examples thereof include agar, alginic acid, carrageenan, xanthan gum, guar gum, locust bean gum, and pectin. However, since pectin exerts its thickening action mainly in a strongly acidic region, the viscosity improving action is hardly exhibited effectively in the pH range of 5.0 to 8.5 as in the present invention. It can be used when producing a low gel type electrolyzed water composition.
上記植物性多糖類は、任意に選ばれる１種もしくは２種以上を組み合わせて用いることができる。 The plant polysaccharides can be used alone or in combination of two or more.
上記植物性多糖類としては、特に好ましくは、寒天を用いるのがよい。寒天は、増粘剤やゲル化剤としての他、食品としても広く普及しており、安価である。また、寒天は、少量の使用で電解水組成物のゲル強度を高めることができる。即ち、本発明のように中性付近の電解水を用いれば、少量の使用でも電解水組成物のゲル強度を充分に高めることができ、形状保持性を確保できる。但し、電解水としてｐＨが５．０を下回る酸性領域の電解水を用いると、ゲル強度を高めるために多量の寒天を含有させる必要があるし、時間が経つに連れてゲル強度が徐々に低下して形状保持性が悪くなる。 As the plant polysaccharide, agar is particularly preferably used. Agar is widely used as a food in addition to a thickener and a gelling agent, and is inexpensive. Agar can increase the gel strength of the electrolyzed water composition with a small amount of use. That is, if the electrolyzed water in the vicinity of neutrality is used as in the present invention, the gel strength of the electrolyzed water composition can be sufficiently increased even with a small amount of use, and shape retention can be ensured. However, if electrolyzed water having an acidic region with a pH lower than 5.0 is used as electrolyzed water, it is necessary to contain a large amount of agar to increase the gel strength, and the gel strength gradually decreases over time. As a result, the shape retention becomes worse.
なお、本発明では、動物由来のゲル化剤（例えば、ゼラチンなど）は用いない。ゼラチンも一般にゲル化剤として用いられるが、ゼラチンはタンパク質であるため、電解水組成物に含まれる有効塩素を分解してしまい、殺菌作用が低下する。 In the present invention, an animal-derived gelling agent (for example, gelatin) is not used. Gelatin is also generally used as a gelling agent, but since gelatin is a protein, it breaks down effective chlorine contained in the electrolyzed water composition, and the bactericidal action is reduced.
本発明の電解水組成物の形態を調整するには、上記植物性多糖類の配合量を調整する代わりに、電解水組成物に水を配合し、この水の量を調整して行ってもよい。 In order to adjust the form of the electrolyzed water composition of the present invention, instead of adjusting the amount of the plant polysaccharide, water may be added to the electrolyzed water composition and the amount of this water adjusted. Good.
本発明の電解水組成物は、有効塩素濃度を１５ｐｐｍ以上とする。有効塩素濃度を１５ｐｐｍ以上とすることで、殺菌作用が発揮され、特に、本発明の電解水組成物を口腔内で使用すると、う蝕原生細菌（ミュータンス菌）を死滅させることができる。 The electrolyzed water composition of the present invention has an effective chlorine concentration of 15 ppm or more. When the effective chlorine concentration is 15 ppm or more, the bactericidal action is exhibited. In particular, when the electrolyzed water composition of the present invention is used in the oral cavity, cariogenic bacteria (mutans bacteria) can be killed.
有効塩素濃度とは、殺菌作用のある塩素（例えば、次亜塩素酸）が含まれている割合を意味し、この有効塩素濃度が１５ｐｐｍを下回ると、殺菌作用が著しく低下する。従って電解水組成物の有効塩素濃度は、１５ｐｐｍ以上とし、好ましくは２５ｐｐｍ以上、より好ましくは３０ｐｐｍ以上、特に好ましくは４０ｐｐｍ以上とする。殺菌作用を高める観点からすれば、有効塩素濃度はできるだけ高い方が好ましいが、有効塩素濃度が高くなり過ぎると、塩素臭が強くなり、患者に不快感を与えることがある。従って電解水組成物の有効塩素濃度は、１０００ｐｐｍ以下とし、好ましくは８００ｐｐｍ以下、より好ましくは６００ｐｐｍ以下、特に好ましくは４００ｐｐｍ以下とする。なお、電解水組成物の有効塩素濃度は、市販されている塩素計（残留塩素計）などを用いて測定すればよい。 The effective chlorine concentration means a ratio in which chlorine having a bactericidal action (for example, hypochlorous acid) is contained. When the effective chlorine concentration is less than 15 ppm, the bactericidal action is remarkably reduced. Therefore, the effective chlorine concentration of the electrolyzed water composition is 15 ppm or more, preferably 25 ppm or more, more preferably 30 ppm or more, and particularly preferably 40 ppm or more. From the viewpoint of enhancing the bactericidal action, it is preferable that the effective chlorine concentration is as high as possible. However, if the effective chlorine concentration is too high, the chlorine odor becomes strong and may cause discomfort to the patient. Therefore, the effective chlorine concentration of the electrolyzed water composition is 1000 ppm or less, preferably 800 ppm or less, more preferably 600 ppm or less, and particularly preferably 400 ppm or less. In addition, what is necessary is just to measure the effective chlorine concentration of an electrolyzed water composition using the commercially available chlorine meter (residual chlorine meter).
更に、本発明の電解水組成物では、有効塩素濃度を１５ｐｐｍ以上に調整したうえで、電解水組成物のｐＨを５．０〜８．５に調整することで、長期間保存した後でも良好な殺菌作用および止血作用を示すようになる。止血作用が発揮される機構は不明であるが、電解水組成物の有効塩素濃度とｐＨの両方が適切な範囲に調整されているため、該電解水組成物を患部に塗布したときに、毛細血管や周辺組織を収縮させて止血作用を促すことを本出願人らは確認している。 Furthermore, in the electrolyzed water composition of the present invention, the effective chlorine concentration is adjusted to 15 ppm or more, and the pH of the electrolyzed water composition is adjusted to 5.0 to 8.5, which is good even after long-term storage. Show bactericidal and hemostatic effects. Although the mechanism by which the hemostatic action is exerted is unknown, since both the effective chlorine concentration and pH of the electrolyzed water composition are adjusted to an appropriate range, when the electrolyzed water composition is applied to the affected area, the capillaries Applicants have confirmed that blood vessels and surrounding tissues are contracted to promote a hemostatic action.
但し、電解水組成物のｐＨが５．０を下回るか、ｐＨが８．５を超えると、殺菌力を有する次亜塩素酸水溶液が安定した状態で存在しないため、殺菌作用が低下したり、長期間保存した後の殺菌作用が低下する。また、止血作用も劣化する。また、特に、電解水組成物のｐＨが５．０を下回り、強酸性になると、本発明の電解水組成物を口腔内で使用したときに、歯の脱灰が促進されたり、歯への侵蝕作用が強くなる。即ち、歯の脱灰や侵蝕は、口腔内に発生する酸が原因であることが知られており、ｐＨが低く、強酸性になると、歯からカルシウムイオンやリン酸イオンが溶け出して歯のエナメル質が白化し、う歯（虫歯）に進展することがある。しかし電解水組成物のｐＨが上記範囲に調整されていれば、歯からのカルシウムイオンやリン酸イオンの溶出を抑えることができ、歯が脱灰されたり、侵蝕される恐れはない。従って電解水組成物のｐＨは、５．０以上とし、好ましくは５．５以上、より好ましくは６．０以上とする。また、ｐＨは８．５以下とし、好ましくは８．０未満とする。 However, when the pH of the electrolyzed water composition is lower than 5.0 or higher than 8.5, since the hypochlorous acid aqueous solution having bactericidal power does not exist in a stable state, the bactericidal action decreases, Bactericidal action after long-term storage is reduced. In addition, the hemostatic action is also deteriorated. In particular, when the pH of the electrolyzed water composition is less than 5.0 and becomes strongly acidic, when the electrolyzed water composition of the present invention is used in the oral cavity, decalcification of teeth is promoted, The erosion action becomes stronger. That is, it is known that tooth decalcification and erosion are caused by acid generated in the oral cavity, and when pH is low and strong acidity, calcium ions and phosphate ions are dissolved from the teeth and the teeth are eroded. Enamel may whiten and develop into caries (cavities). However, if the pH of the electrolyzed water composition is adjusted to the above range, elution of calcium ions and phosphate ions from the teeth can be suppressed, and there is no fear that the teeth are decalcified or eroded. Therefore, the pH of the electrolyzed water composition is 5.0 or more, preferably 5.5 or more, more preferably 6.0 or more. Moreover, pH shall be 8.5 or less, Preferably it shall be less than 8.0.
上記電解水組成物のｐＨは、原料として用いる電解水のｐＨにほぼ依存するが、該電解水に配合する植物性多糖類の種類にも影響を受ける。そこで電解水組成物のｐＨを上記範囲に調整するには、電解水のｐＨを調整したり、複数種類の植物性多糖類を配合したり、市販されているｐＨ調整剤を配合すればよい。ｐＨ調整剤としては、例えば、炭酸ナトリウムなどの弱アルカリ性を示す添加物や、酢酸などの弱酸性を示す添加物が挙げられる。 The pH of the electrolyzed water composition is substantially dependent on the pH of electrolyzed water used as a raw material, but is also affected by the type of vegetable polysaccharide to be blended in the electrolyzed water. Therefore, in order to adjust the pH of the electrolyzed water composition to the above range, the pH of the electrolyzed water may be adjusted, a plurality of types of plant polysaccharides may be blended, or a commercially available pH adjuster may be blended. Examples of the pH adjuster include an additive exhibiting weak alkalinity such as sodium carbonate and an additive exhibiting weak acidity such as acetic acid.
本発明の電解水組成物を製造するには、原料として塩化ナトリウム含有水溶液を電気分解して得られる電解水を用いればよく、特に、ｐＨが５．０〜８．５の電解水を用いるのが好ましい。塩化ナトリウム含有水溶液を電気分解することで、有効塩素を含有する電解水を容易に得ることができ、特に、ｐＨが５．０〜８．５の電解水を原料として用いることで、最終的に得られる電解水組成物のｐＨを５．０〜８．５の範囲に容易に調整できる。 In order to produce the electrolyzed water composition of the present invention, electrolyzed water obtained by electrolyzing a sodium chloride-containing aqueous solution as a raw material may be used. In particular, electrolyzed water having a pH of 5.0 to 8.5 is used. Is preferred. By electrolyzing the sodium chloride-containing aqueous solution, electrolyzed water containing effective chlorine can be easily obtained, and in particular, by using electrolyzed water having a pH of 5.0 to 8.5 as a raw material, finally, The pH of the obtained electrolyzed water composition can be easily adjusted in the range of 5.0 to 8.5.
上記電解水の有効塩素濃度は、１５ｐｐｍ以上とすることが好ましい。最終的に得られる電解水組成物の有効塩素濃度を１５ｐｐｍ以上に調整しやすくするためである。 The effective chlorine concentration of the electrolyzed water is preferably 15 ppm or more. This is because the effective chlorine concentration of the finally obtained electrolyzed water composition can be easily adjusted to 15 ppm or more.
また、電解水に残留する塩化ナトリウム濃度は、０．２％（２０００ｐｐｍ）程度以下であればよい。食塩の濃縮に起因する痛み等の苦痛を低減するためである。 Further, the concentration of sodium chloride remaining in the electrolyzed water may be about 0.2% (2000 ppm) or less. This is to reduce pain such as pain caused by concentration of salt.
また、上記電解水の酸化還元電位は、例えば、６００〜１０００ｍＶ程度であることが好ましい。 The redox potential of the electrolyzed water is preferably about 600 to 1000 mV, for example.
原料として用いる電解水のｐＨや有効塩素濃度、残留塩化ナトリウム濃度、酸化還元電位は、最終的に得られる上記電解水組成物の用途に応じて適宜調整すればよいが、好ましくは、ｐＨは６．０以上、８．０未満、有効塩素濃度は３０ｐｐｍ以上、０．０６％未満、残留塩化ナトリウム濃度は０．１％（１０００ｐｐｍ）以下、酸化還元電位は７００〜９００ｍＶであるのがよい。 The pH, effective chlorine concentration, residual sodium chloride concentration, and redox potential of the electrolyzed water used as a raw material may be appropriately adjusted according to the use of the electrolyzed water composition finally obtained, but preferably the pH is 6 0.0 or more and less than 8.0, effective chlorine concentration is 30 ppm or more and less than 0.06%, residual sodium chloride concentration is 0.1% (1000 ppm) or less, and oxidation-reduction potential is preferably 700 to 900 mV.
塩化ナトリウム含有水溶液を電気分解して得られる電解水は、例えば、上記特許文献３や特許文献４に開示した技術によって製造することができる。即ち、塩化ナトリウム含有水溶液を無隔膜電解槽で電気分解し、生成した電解液を更に隔膜電解槽の陽極側に導入すると共に、該隔膜電解槽の陰極側に、塩化ナトリウム含有水溶液を導入し、これを電気分解すれば、陽極側にｐＨが５．０〜８．５の電解水を生成させることができる。また、無隔膜電解して得られた電解水を隔膜電解して得られた電解水と、例えば、無隔膜電解して得られた電解水や、隔膜電解して得られた電解水を混合してもよい。なお、詳細は、上記特許文献３や特許文献４に記載した方法を参照すればよい。 Electrolyzed water obtained by electrolyzing a sodium chloride-containing aqueous solution can be produced by the techniques disclosed in Patent Document 3 and Patent Document 4, for example. That is, the sodium chloride-containing aqueous solution is electrolyzed in a non-diaphragm electrolytic cell, and the generated electrolytic solution is further introduced to the anode side of the diaphragm electrolytic cell, and the sodium chloride-containing aqueous solution is introduced to the cathode side of the diaphragm electrolytic cell, If this is electrolyzed, electrolyzed water having a pH of 5.0 to 8.5 can be generated on the anode side. Also, the electrolyzed water obtained by subjecting electrolyzed water obtained by electroless membrane electrolysis to electrolyzed membrane to electrolyzed water, for example, electrolyzed water obtained by electroless membrane electrolysis, or electrolyzed water obtained by electrolyzing the membrane is mixed. May be. For details, the methods described in Patent Document 3 and Patent Document 4 may be referred to.
塩化ナトリウム含有水溶液を電気分解して得られる電解水と、上記植物性多糖類を配合するにあたっては、これらを直接配合し、必要に応じて更に水を加えることによって植物性多糖類の含有量を調整してもよいし、或いは予め植物性多糖類を水に分散させて植物性多糖類含有水溶液とし、この水溶液と上記電解水とを混合し、必要に応じて更に水を加えることによって植物性多糖類の含有量を調整してもよい。また、必要に応じて、ｐＨ調整剤を添加してもよい。本発明において、水としては、イオン交換水を用いることが好ましい。 In blending electrolyzed water obtained by electrolyzing a sodium chloride-containing aqueous solution and the above-mentioned plant polysaccharide, these are directly blended, and the content of the plant polysaccharide can be adjusted by adding water as necessary. The plant polysaccharide may be prepared by previously dispersing the plant polysaccharide in water to obtain a plant polysaccharide-containing aqueous solution, mixing the aqueous solution with the electrolytic water, and adding water as necessary. You may adjust content of a polysaccharide. Moreover, you may add a pH adjuster as needed. In the present invention, it is preferable to use ion exchange water as water.
本発明の電解水組成物は、上記電解水と植物性多糖類の他に、種々の添加剤を配合してもよい。添加剤としては、香料や補助機能剤などを配合してもよい。 The electrolyzed water composition of the present invention may contain various additives in addition to the electrolyzed water and the vegetable polysaccharide. As an additive, a fragrance or an auxiliary function agent may be blended.
補助機能剤としては、特に、歯の脱灰を防止するために、カルシウムやヒドロキシアパタイト、キシリトールなどを配合してもよい。特に、植物性多糖類として、アルギン酸やカラギーナンを用いる場合は、カルシウムを併用することが推奨される。アルギン酸やカラギーナンのカルシウム塩は、血液に触れるとカルシウムを放出して止血作用を一段と向上させることができるからである。 As an auxiliary function agent, calcium, hydroxyapatite, xylitol, or the like may be blended to prevent tooth decalcification. In particular, when alginic acid or carrageenan is used as the plant polysaccharide, it is recommended to use calcium together. This is because calcium salts of alginic acid and carrageenan release calcium when touched with blood and can further improve the hemostatic action.
本発明の電解水組成物は、外科臨床における術後処理や創傷処理、或いは口腔内に発生した出血を止血する際に用いることができ、医療現場のみならず、一般家庭内でも使用することができる。口腔内では、例えば、抜歯窩等の洗浄や、根管治療等への適用、或いは口腔内に発生した擦過傷の治療等への適用が可能となる。特に本発明の電解水組成物は植物性多糖類を配合することで粘度が高められているため、患部に長時間留まり、殺菌作用および止血作用が長時間にわたって継続して発揮される。また、本発明の電解水組成物のｐＨは中性付近に調整されているため、歯を脱灰したり、歯を侵蝕する作用を有していないため、口腔内での使用に非常に適している。 The electrolyzed water composition of the present invention can be used for postoperative treatment and wound treatment in surgical clinics, or when bleeding in the oral cavity is stopped, and can be used not only in a medical field but also in general homes. it can. In the oral cavity, for example, it can be applied to cleaning the tooth extraction socket, application to root canal treatment, etc., or treatment of abrasions generated in the oral cavity. In particular, since the viscosity of the electrolyzed water composition of the present invention is increased by blending the plant polysaccharide, it stays in the affected area for a long time, and the bactericidal action and the hemostatic action are continuously exhibited for a long time. In addition, since the pH of the electrolyzed water composition of the present invention is adjusted to near neutrality, it does not have the effect of decalcifying teeth or eroding teeth, so it is very suitable for use in the oral cavity. ing.
以下、本発明を実施例によって更に詳細に説明するが、下記実施例は本発明を限定する性質のものではなく、前・後記の趣旨に適合し得る範囲で適当に変更して実施することも可能であり、それらはいずれも本発明の技術的範囲に含まれる。 Hereinafter, the present invention will be described in more detail with reference to examples. However, the following examples are not intended to limit the present invention, and may be implemented with appropriate modifications within a range that can meet the purpose described above and below. These are all possible and are within the scope of the present invention.
下記の手順で電解水と多糖類含有水溶液を夫々調製し、これらを混合して供試材を調製した。 Electrolyzed water and a polysaccharide-containing aqueous solution were prepared by the following procedure, and these were mixed to prepare a test material.
＜電解水の調製＞
［電解水１］
無隔膜電解槽に、塩化ナトリウムを５％含む水溶液を流速１０ｍｌ／分で供給しつつ、電流を３Ａとして無隔膜電解を行ない、ｐＨが１１で、有効塩素濃度が４５００ｐｐｍのアルカリ性電解水を得た。得られたアルカリ性電解水を、引続き、有隔膜電解槽の陽極側へ流速１０ｍｌ／分で供給し、一方、陰極側には上記５％塩化ナトリウム水溶液を流速１０ｍｌ／分で供給して電流を３Ａとして隔膜電解を行った。その結果、陽極側から、ｐＨが５．２で、有効塩素濃度が９０００ｐｐｍの電解水を得た。
<Preparation of electrolyzed water>
[Electrolyzed water 1]
While supplying an aqueous solution containing 5% sodium chloride to the diaphragm electrolyzer at a flow rate of 10 ml / min, the diaphragm was electrolyzed with a current of 3 A, and an alkaline electrolyzed water having a pH of 11 and an effective chlorine concentration of 4500 ppm was obtained. . The obtained alkaline electrolyzed water was continuously supplied to the anode side of the diaphragm electrolytic cell at a flow rate of 10 ml / min, while the 5% sodium chloride aqueous solution was supplied to the cathode side at a flow rate of 10 ml / min to supply a current of 3 A. Diaphragm electrolysis was performed. As a result, electrolyzed water having a pH of 5.2 and an effective chlorine concentration of 9000 ppm was obtained from the anode side.
陽極側から流速１０ｍｌ／分で得られた電解水に、流速３Ｌ／分でイオン交換水を供給して希釈し、ｐＨが６．５で有効塩素濃度が５００ｐｐｍの希釈電解水を得た。得られた希釈電解水を「電解水１」とする。得られた希釈電解水（電解水１）の残留塩化ナトリウム濃度は１２００ｐｐｍであり、酸化還元電位は８００ｍＶであった。 The electrolyzed water obtained at a flow rate of 10 ml / min from the anode side was diluted by supplying ion-exchanged water at a flow rate of 3 L / min to obtain diluted electrolyzed water having a pH of 6.5 and an effective chlorine concentration of 500 ppm. The obtained diluted electrolyzed water is referred to as “electrolyzed water 1”. The resulting diluted electrolyzed water (electrolyzed water 1) had a residual sodium chloride concentration of 1200 ppm and an oxidation-reduction potential of 800 mV.
［電解水２］
無隔膜電解槽に、塩化ナトリウムを５％含む水溶液を流速１０ｍｌ／分で供給しつつ、電流を４Ａとして無隔膜電解を行ない、ｐＨが１３で、有効塩素濃度が８０００ｐｐｍのアルカリ性電解水を得た。得られたアルカリ性電解水を、引続き、有隔膜電解槽の陽極側へ流速１０ｍｌ／分で供給し、一方、陰極側には上記５％塩化ナトリウム水溶液を流速１０ｍｌ／分で供給して電流を４Ａとして隔膜電解を行った。その結果、陽極側から、ｐＨが４．１で、有効塩素濃度が１６０００ｐｐｍの電解水ａを得た。
[Electrolyzed water 2]
While supplying an aqueous solution containing 5% sodium chloride to the diaphragm electrolyzer at a flow rate of 10 ml / min, the diaphragm was electrolyzed at a current of 4 A, and an alkaline electrolyzed water having a pH of 13 and an effective chlorine concentration of 8000 ppm was obtained. . The obtained alkaline electrolyzed water was continuously supplied to the anode side of the diaphragm electrolytic cell at a flow rate of 10 ml / min, while the 5% sodium chloride aqueous solution was supplied to the cathode side at a flow rate of 10 ml / min to supply a current of 4 A. Diaphragm electrolysis was performed. As a result, electrolyzed water a having a pH of 4.1 and an effective chlorine concentration of 16000 ppm was obtained from the anode side.
一方、無隔膜電解槽に、塩化ナトリウムを５％含む水溶液を流速１５ｍｌ／分で供給しつつ、電流を１０Ａとして無隔膜電解を行ない、ｐＨが１３で、有効塩素濃度が１４０００ｐｐｍのアルカリ性電解水ｂを得た。 On the other hand, while supplying an aqueous solution containing 5% sodium chloride to the diaphragm electrolyzer at a flow rate of 15 ml / min, the diaphragm was electrolyzed at a current of 10 A, the pH was 13, and the alkaline electrolyzed water b having an effective chlorine concentration of 14000 ppm was used. Got.
陽極側から流速１０ｍｌ／分で得られた電解水ａと、上記無隔膜電解槽から流速１５ｍｌ／分で得られたアルカリ性電解水ｂを混合すると共に、この混合液に、更に流速３Ｌ／分でイオン交換水を供給して希釈し、ｐＨが８．０で有効塩素濃度が５００ｐｐｍの希釈電解水を得た。得られた希釈電解水を「電解水２」とする。得られた希釈電解水（電解水２）の残留塩化ナトリウム濃度は３００ｐｐｍであり、酸化還元電位は７５０ｍＶであった。 Electrolyzed water a obtained from the anode side at a flow rate of 10 ml / min and alkaline electrolyzed water b obtained from the non-diaphragm electrolytic cell at a flow rate of 15 ml / min were mixed, and this mixture was further mixed at a flow rate of 3 L / min. Ion exchanged water was supplied for dilution to obtain diluted electrolyzed water having a pH of 8.0 and an effective chlorine concentration of 500 ppm. The obtained diluted electrolyzed water is referred to as “electrolyzed water 2”. The obtained diluted electrolyzed water (electrolyzed water 2) had a residual sodium chloride concentration of 300 ppm and an oxidation-reduction potential of 750 mV.
［強酸性電解水］
有隔膜電解槽の陽極側と陰極側に、塩化ナトリウムを０．０５％含む水溶液を流速１５００ｍｌ／分で供給しつつ、電流を５Ａとして隔膜電解を行った。その結果、陽極側から、ｐＨが２．３で、有効塩素濃度が１００ｐｐｍの電解水を得た。得られた電解水を「強酸性電解水」とする。得られた強酸性電解水の残留塩化ナトリウム濃度は３５０ｐｐｍであり、酸化還元電位は１１００ｍＶであった。
[Strongly acidic electrolyzed water]
While supplying an aqueous solution containing 0.05% sodium chloride at a flow rate of 1500 ml / min to the anode side and the cathode side of the diaphragm electrolyzer, diaphragm electrolysis was performed at a current of 5A. As a result, electrolyzed water having a pH of 2.3 and an effective chlorine concentration of 100 ppm was obtained from the anode side. The obtained electrolyzed water is referred to as “strongly acidic electrolyzed water”. The residual sodium chloride concentration of the obtained strongly acidic electrolyzed water was 350 ppm, and the oxidation-reduction potential was 1100 mV.
＜多糖類含有水溶液の調製＞
植物性多糖類として、寒天、アルギン酸、カラギーナン、キサンタンガム、ローカストビーンガム、グアーガムを用い、動物性多糖類として、ゼラチンを用い、多糖類を含有する水溶液を下記手順で調製した。
<Preparation of polysaccharide-containing aqueous solution>
Agar, alginic acid, carrageenan, xanthan gum, locust bean gum, and guar gum were used as plant polysaccharides, gelatin was used as animal polysaccharides, and an aqueous solution containing the polysaccharides was prepared by the following procedure.
［寒天含有水溶液］
食品用粉寒天（株式会社朝日製の粉寒天）を水と混合し、これを加熱して溶解し、寒天を１％含有する水溶液を調製した。
[Agar-containing aqueous solution]
Food agar (Asahi Co., Ltd. powder agar) was mixed with water and dissolved by heating to prepare an aqueous solution containing 1% agar.
［アルギン酸含有水溶液］
食品用アルギン酸ソーダ（株式会社紀文フードケミファ製の「ダッグアルギン（商品名）」）を水と混合し、これを加熱して溶解し、アルギン酸を３％含有する水溶液を調製した。
[Alginic acid-containing aqueous solution]
Sodium alginate for food ("Dag Argin (trade name)" manufactured by Kibun Food Chemifa Co., Ltd.) was mixed with water and dissolved by heating to prepare an aqueous solution containing 3% alginic acid.
［カラギーナン含有水溶液］
食品用カラギーナン（ユニテックフーズ株式会社製の「ＳＡＴＩＡＧＥＬ（商品名）」）を水と混合し、８０℃に加熱して溶解させ、カラギーナンを１％含有する水溶液を調製した。
[Carrageenan-containing aqueous solution]
Carrageenan for food (“SATIAGEL (trade name)” manufactured by Unitech Foods Co., Ltd.) was mixed with water and dissolved by heating to 80 ° C. to prepare an aqueous solution containing 1% of carrageenan.
［キサンタンガム含有水溶液］
食品用キサンタンガム（ユニテックフーズ株式会社製の「ＳＡＴＩＡＸＡＮＥ（商品名）」）を水と混合して溶解させ、キサンタンガムを２％含有する水溶液を調製した。
[Aqueous solution containing xanthan gum]
Xanthan gum for food ("SATIAXANE (trade name)" manufactured by Unitech Foods Co., Ltd.) was mixed with water and dissolved to prepare an aqueous solution containing 2% xanthan gum.
［ローカストビーンガム含有水溶液］
食品用ローカストビーンガム（三晶株式会社製の「ゲニューガム（商品名）」）を水と混合し、これを加熱して溶解させ、ローカストビーンガムを２％含有する水溶液を調整した。
[Locust bean gum-containing aqueous solution]
Locust bean gum for food (“Genu gum (trade name)” manufactured by Sanki Co., Ltd.) was mixed with water and dissolved by heating to prepare an aqueous solution containing 2% locust bean gum.
［グアーガム含有水溶液］
食品用グアーガム（ユニテックフーズ株式会社製の「ＶＩＤＯＧＵＭＧＨＫ（商品名）」）を水と混合して溶解させ、グアーガムを２％含有する水溶液を調製した。
[Aqueous solution containing guar gum]
A guar gum for food (“VIDOGUMGHK (trade name)” manufactured by Unitech Foods Co., Ltd.) was mixed with water and dissolved to prepare an aqueous solution containing 2% guar gum.
［ゼラチン含有水溶液］
食品用ゼラチン（ユニテックフーズ株式会社製の「ゼラチンＰＳ（商品名）」）を水と混合して溶解させ、ゼラチンを１％含有する水溶液を調製した。
[Aqueous solution containing gelatin]
Gelatin for food ("Gelatin PS (trade name)" manufactured by Unitech Foods Co., Ltd.) was mixed with water and dissolved to prepare an aqueous solution containing 1% gelatin.
＜供試材の調製＞
上記電解水と、上記多糖類含有水溶液と、必要に応じて水と、必要に応じて添加剤（第２リン酸カルシウム）を、質量基準で、下記表１に示す割合で混合して供試材を調製した。
<Preparation of test material>
The above electrolyzed water, the above polysaccharide-containing aqueous solution, water as required, and optionally an additive (secondary calcium phosphate) are mixed at a ratio shown in Table 1 below on a mass basis, and a test material is prepared. Prepared.
なお、多糖類含有水溶液として、寒天含有水溶液、カラギーナン含有水溶液、ローカストビーンガム含有水溶液を用いた場合は、該水溶液の温度が６０℃を下回らないうちに電解水や水と混合してから室温まで冷却して供試材を調製した。 In addition, when an agar-containing aqueous solution, a carrageenan-containing aqueous solution, or a locust bean gum-containing aqueous solution is used as the polysaccharide-containing aqueous solution, it is mixed with electrolyzed water or water before the temperature of the aqueous solution falls below 60 ° C. until the room temperature is reached. A specimen was prepared by cooling.
また、下記表１のＮｏ．１０については、電解水２とアルギン酸水溶液を混合する際に、補助機能剤として第２リン酸カルシウムを添加し、これらの混合作業を１０分以内に行なって供試材を調製した。 In addition, No. 1 in Table 1 below. Regarding No. 10, when mixing the electrolyzed water 2 and the aqueous alginate solution, dibasic calcium phosphate was added as an auxiliary functioning agent, and these mixing operations were performed within 10 minutes to prepare a specimen.
下記表１のＮｏ．１４とＮｏ．１５については、上記強酸性電解水５０ｇを５０℃に加熱した後、この電解水を、９０℃に加熱した上記寒天含有水溶液５０ｇに攪拌しながら添加し、これを室温まで冷却して供試材を調製した。 No. in Table 1 below. 14 and no. For No. 15, after heating 50 g of the strongly acidic electrolyzed water to 50 ° C., this electrolyzed water was added to 50 g of the agar-containing aqueous solution heated to 90 ° C. with stirring, and this was cooled to room temperature and tested. Was prepared.
得られた供試材に占める多糖類の配合量（濃度）と供試材に含まれる有効塩素濃度を下記表１に示す。多糖類の濃度は、乾燥重量で算出した。有効塩素濃度は、市販されている残留塩素計を用いて測定した。 Table 1 below shows the blending amount (concentration) of the polysaccharide in the obtained test material and the effective chlorine concentration contained in the test material. The concentration of polysaccharide was calculated by dry weight. The effective chlorine concentration was measured using a commercially available residual chlorine meter.
得られた供試材について、形状保持性、殺菌作用、殺菌作用の持続性、止血作用を下記手順で夫々評価した。また、得られた供試材を口腔内で使用することを想定して、歯に対する脱灰・侵蝕性についても下記手順で評価した。 The obtained specimens were evaluated for shape retention, bactericidal action, persistence of bactericidal action, and hemostatic action by the following procedures. In addition, assuming that the obtained test material is used in the oral cavity, decalcification / erosion on teeth was also evaluated by the following procedure.
［形状保持性］
各供試材について、ゲル状のものは供試材のゲル強度を、ジェル状のものは供試材の粘度を夫々測定した。供試材のゲル強度と粘度は、供試材を調製した後、２時間以内に測定した。ゲル強度は、上述した日寒水式の方法で測定し、粘度は、Ｂ型回転粘度計を用いて測定した。測定結果を下記表２に示す。
[Shape retention]
About each test material, the gel-like thing measured the gel strength of the test material, and the gel-like thing measured the viscosity of the test material, respectively. The gel strength and viscosity of the test material were measured within 2 hours after preparing the test material. The gel strength was measured by the above-mentioned Nissui method, and the viscosity was measured using a B-type rotational viscometer. The measurement results are shown in Table 2 below.
供試材の形状保持性は、チューブに詰めた供試材をガラス板の上に内径５ｍｍφの口から１ｃｍほど押し出したものを、３７℃の恒温槽に入れ、３０分間静置し、３０分経過後の形状変化を観察して評価した。 The shape retention of the test material was obtained by extruding the test material packed in a tube about 1 cm from the mouth with an inner diameter of 5 mmφ on a glass plate, placing it in a constant temperature bath at 37 ° C., and allowing it to stand for 30 minutes. The shape change after the lapse was observed and evaluated.
３０分経過しても、液成分がほとんど浸み出しておらず、形状が何ら変化していないか、液成分は多少浸み出しているものの形状がほぼ変化していない場合を合格（○）とし、形状が保持されていない場合を不合格（×）として評価した。評価結果を下記表２に示す。 Even if 30 minutes have passed, the liquid component has hardly oozed out, and the shape has not changed at all, or the liquid component has oozed out somewhat but the shape has not changed substantially (○) And the case where the shape was not retained was evaluated as a failure (x). The evaluation results are shown in Table 2 below.
［殺菌作用の評価］
黄色ブドウ球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ ２０９Ｐ）を培養液（ＢＨＩ；ブレイン・ハート・インフュージョン・ブロス培地）中で、３７℃、２４時間培養し、遠心分離して集菌した後、ＢＨＩに分散させて１．０×１０8個／ｍｌの濃度に調整した。この菌液０．１ｍｌに、供試材９．９ｍｌを混合して３分間静置した。静置後、１ｍｌを採取し、普通寒天培地（日水製薬製）と混釈後、３７℃、２４時間培養した。培養後、培地上のコロニー数から、処理後に残存する生菌数を算出した。生菌数を下記表２に示す。生菌数が１０3個未満のものを殺菌作用有りとして合格（○）と評価し、１０3個以上のものを殺菌作用無しとして不合格（×）と評価した。
[Evaluation of bactericidal action]
Staphylococcus aureus 209P is cultured in a culture solution (BHI; Brain Heart Infusion Broth Medium) at 37 ° C. for 24 hours, collected by centrifugation, dispersed in BHI, 1 The concentration was adjusted to 0.0 × 10 8 cells / ml. 9.9 ml of the test material was mixed with 0.1 ml of this bacterial solution and allowed to stand for 3 minutes. After standing, 1 ml was collected and mixed with a normal agar medium (manufactured by Nissui Pharmaceutical), followed by incubation at 37 ° C. for 24 hours. After culturing, the number of viable bacteria remaining after the treatment was calculated from the number of colonies on the medium. The number of viable bacteria is shown in Table 2 below. Those having a viable count of less than 10 3 were evaluated as acceptable (◯) as having a bactericidal action, and those having 10 3 or more were evaluated as rejecting (×) as having no bactericidal action.
［殺菌作用の持続性］
各供試材を、空気に触れない状態で室温で５日間遮光密閉保存した後、上記［殺菌作用の評価］の項で示した手順で殺菌作用を評価した。
[Persistence of bactericidal action]
Each test material was stored in a light-tight sealed state at room temperature for 5 days without being exposed to air, and then the bactericidal action was evaluated according to the procedure shown in the above section [Evaluation of bactericidal action].
生菌数を下記表２に示す。生菌数が１０3個未満のものを殺菌作用の持続性有りとして合格（○）と評価し、１０3個以上のものを殺菌作用の持続性無しとして不合格（×）と評価した。 The number of viable bacteria is shown in Table 2 below. Those having a viable count of less than 10 3 were evaluated as acceptable (◯) as having a bactericidal effect, and those having 10 3 or more were evaluated as rejected (×) as having no bactericidal activity.
［止血作用の評価］
５週齢のＩＣＲ系ＳＰＦマウス（体重約３０ｇ）を用い、以下のようにして止血作用を評価した。
[Evaluation of hemostasis]
A 5-week-old ICR-type SPF mouse (body weight of about 30 g) was used to evaluate the hemostatic effect as follows.
まず、実験開始前１週間は、マウスを室温の恒温室で明暗条件を１２時間ずつとして飼育し、飼育中は同じ餌と水を自由に与えた。次に、マウスに全身麻酔を施し、尾尖端から１０ｍｍの位置をかみそりで切断し、切断創を含む尾を、供試材を入れたシャーレに垂直に差し込み、３０秒保持した。シャーレには、供試材が厚さ１０ｍｍとなるように充填されており、切断創を含む尾をシャーレの底まで差し込んだ。 First, for one week before the start of the experiment, the mice were bred in a constant temperature room at room temperature for 12 hours, and the same food and water were freely given during the breeding. Next, the mouse was subjected to general anesthesia, the position 10 mm from the tip of the tail was cut with a razor, and the tail including the cut wound was vertically inserted into the petri dish containing the test material and held for 30 seconds. The petri dish was filled with the test material to a thickness of 10 mm, and the tail including the cut wound was inserted to the bottom of the petri dish.
３０秒保持後、尾をシャーレから引き上げた後、切断創から出血した血液を３０秒ごとにろ紙で吸い取った。血液を吸い取る際には、切断創に付着している供試材が可能な限りろ紙に付着しないように慎重に行なった。血液の吸い取りは、ろ紙に血液が付着しなくなるまで続け、血液が付着しなくなるまでに要した時間を測定した。実験に使用したマウスは、各供試材について５匹ずつとした。 After holding for 30 seconds, the tail was lifted from the petri dish, and blood bleeding from the cut wound was sucked with a filter paper every 30 seconds. When sucking blood, the test material adhering to the cut wound was carefully taken so as not to adhere to the filter paper as much as possible. The blood sucking was continued until blood did not adhere to the filter paper, and the time required until blood did not adhere was measured. Five mice were used for each test material used in the experiment.
なお、参考実験として、尾尖端を切断した後、何もせず、そのまま放置したときに止血するまでに要する時間を上記手順で同様に測定した。その結果、止血までに６分±１．５分かかった。 In addition, as a reference experiment, after cutting the caudal tip, the time required to stop hemostasis when nothing was done and was left as it was was measured in the same manner as described above. As a result, it took 6 minutes ± 1.5 minutes to stop bleeding.
また、参考実験として、シャーレに止血剤として知られているボスミン（商品名；第一工業製薬製）液を入れて同様の実験を行なった。その結果、止血までに１．５分±０．５分かかった。 In addition, as a reference experiment, a similar experiment was performed by putting a bosmin (trade name; manufactured by Daiichi Kogyo Seiyaku) known as a hemostatic agent into a petri dish. As a result, it took 1.5 minutes ± 0.5 minutes to stop bleeding.
［歯に対する脱灰・侵蝕性の評価］
表１の供試材Ｎｏ．７（本発明例）と供試材Ｎｏ．１４（比較例）を用い、以下の手順で脱灰・侵蝕性試験を行った。
[Evaluation of decalcification / erosion on teeth]
Sample No. in Table 1 7 (example of the present invention) and test material No. 14 (Comparative Example) was used to perform a decalcification / erosion test according to the following procedure.
６週齢のｄｄｙ系雄性マウスから下顎骨ごと歯を摘出し、正中線から左右に分け、右側の下顎骨を供試材Ｎｏ．７またはＮｏ．１４を５０ｇ充填した１００ｃｃの蓋付きガラス容器に入れ、軽く攪拌し、浸漬した後３０秒振盪脱泡し、３７℃の恒温槽で保持した。２日、５日、７日目毎に下顎骨を取り出し、歯の形態を走査型電子顕微鏡で観察した。下顎骨を取り出したときには、ガラス容器内に充填した供試材を新しいものに交換した。一方、左側の下顎骨は、比較対象として、寒天を０．５％含有する水溶液（ジェル）に浸漬し、上記手順で脱灰・侵蝕性試験を行った。 The teeth along with the mandible were removed from a 6-week-old ddy male mouse, divided into left and right from the midline, and the right mandible was used as the test material No. 7 or No. The sample was put in a 100 cc glass container with a lid filled with 50 g of 14 and lightly stirred, immersed, shaken and degassed for 30 seconds, and held in a thermostatic bath at 37 ° C. The mandible was taken out every 2nd, 5th and 7th days, and the morphology of the teeth was observed with a scanning electron microscope. When the mandible was taken out, the specimen filled in the glass container was replaced with a new one. On the other hand, the left mandible was immersed in an aqueous solution (gel) containing 0.5% agar as a comparison object, and a decalcification / erosion test was performed according to the above procedure.
下記表１において、空欄は添加していないことを意味し、下記表２において、空欄は実験を行なっていないことを意味する。 In Table 1 below, a blank means that no addition was made, and in Table 2 below, a blank means that no experiment was performed.
表１と表２から次のように考察できる。Ｎｏ．１〜１０は、本発明で規定する要件を満足する例であり、全ての特性について優れている。特に、表２から明らかなように、本発明で規定する要件を満足する供試材（Ｎｏ．１〜５，Ｎｏ．７〜１０）を用いた場合は、尾尖端を切断した後、何もせず、そのまま放置した場合（止血までにかかる時間は、６分±１．５分）と比べて止血作用が良好に発揮されていることが分かる。また、本発明の供試材の止血作用は、止血剤として知られているボスミン液と同程度であることが分かる。 From Table 1 and Table 2, it can be considered as follows. No. Examples 1 to 10 are examples that satisfy the requirements defined in the present invention, and are excellent in all characteristics. In particular, as is apparent from Table 2, when using test materials (No. 1 to 5, No. 7 to 10) that satisfy the requirements defined in the present invention, do nothing after cutting the tail tip. However, it can be seen that the hemostasis effect is better than when it is left as it is (the time required for hemostasis is 6 minutes ± 1.5 minutes). Moreover, it turns out that the hemostatic action of the test material of this invention is comparable as the bosmin liquid known as a hemostatic agent.
また、供試材Ｎｏ．７に浸漬した下顎骨の歯は、７日経過しても形態に変化が全く認められなかった。 In addition, specimen No. No change in the morphology of the mandibular teeth immersed in 7 was observed after 7 days.
一方、Ｎｏ．１１〜１５は、本発明で規定するいずれかの要件を満たさない例であり、いずれかの特性が劣っている。 On the other hand, no. 11 to 15 are examples that do not satisfy any of the requirements defined in the present invention, and any of the characteristics is inferior.
Ｎｏ．１１は、供試材に占める植物性多糖類の配合量が本発明で規定する範囲より少ないため、形状保持性が悪い。Ｎｏ．１２は、供試材の有効塩素濃度が本発明で規定する範囲より低いため、殺菌作用が悪い。Ｎｏ．１３は、動物性多糖類を用いた例であり、供試材に含まれる有効塩素がタンパク質によって分解されたため、殺菌作用が発揮されていない。Ｎｏ．１４とＮｏ．１５は、強酸性電解水を用いた例であり、殺菌作用が持続されないか、形状保持性が悪くなっている。 No. No. 11 has poor shape retention because the blending amount of the plant polysaccharide in the test material is less than the range defined in the present invention. No. No. 12 has a poor bactericidal action because the effective chlorine concentration of the test material is lower than the range defined in the present invention. No. 13 is an example using animal polysaccharides, and the effective chlorine contained in the test material was decomposed by the protein, so that the bactericidal action was not exhibited. No. 14 and no. No. 15 is an example using strongly acidic electrolyzed water, and the bactericidal action is not maintained or the shape retention is deteriorated.
また、特に表２から明らかなように、Ｎｏ．１１〜１４は、本発明の供試材よりも止血までに時間がかかり、止血作用が劣っていることが分かる。 In addition, as clearly shown in Table 2, no. It can be seen that Nos. 11 to 14 require more time for hemostasis than the test material of the present invention, and the hemostasis action is inferior.
供試材Ｎｏ．１４に浸漬した下顎骨の歯は、２日経過後には歯の表層のエナメル質に脱灰が認められ、７日経過後には、歯の形態をとどめない程に侵蝕が観察された。 Specimen No. The teeth of the mandible soaked in 14 were decalcified in the enamel of the surface of the teeth after 2 days, and after 7 days, the erosion was observed to the extent that the shape of the teeth was not retained.
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