JP2008238097A - Minute flow passage assembly apparatus for producing droplet - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a minute flow passage assembly apparatus for producing droplets which uses a flow passage structure for producing fine particle, the structure being manufactured inexpensively and in large quantities, and can produce a product in large quantities by arranging minute flow passages three-dimensionally at an increased degree of integration and sending a fluid uniformly to all of the minute flow passages. <P>SOLUTION: The minute flow passage structure has a fluid introduction port for introducing the fluid, minute flow passages for producing fine particles from the fluid and a fluid discharge port for discharging the fluid containing the produced fine particles and the minute flow passage structure is composed of a fluid supply structure for supplying the fluid to minute flow passages, a minute flow passage substrate having minute flow passages and a plate interposed between the fluid supply structure and the minute flow passage substrate. A base material of the minute flow passage substrate is a resin which has ≥70 hardness in the type D when measured by the durometer hardness test method according to JIS K 6253 and ≤3% mold shrinkage rate when measured according to JIS K 7152. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a manufacturing apparatus and a manufacturing method for gels and microcapsules having a uniform particle diameter.

  In recent years, a microchannel structure having a microchannel having a length of about several cm and a width and depth of several μm to several hundreds of μm on a glass substrate of several cm square is used to send a fluid to the microchannel. Research to produce chemical synthetic substances by chemical treatment by liquefaction (see, for example, Patent Document 1 and Non-Patent Document 1) and research to produce fine particles (see, for example, Patent Document 2, Patent Document 3, and Non-Patent Document 2) ) Is attracting attention. Here, the microchannel generally refers to a channel having a channel width of 1 μm to 500 μm, a channel depth of 0.1 μm to 200 μm, and a channel length of 1 μm to 50 cm. In addition, the flow path described here means a micro flow path, a supply flow path communicating with the micro flow path, a distribution flow path, a discharge flow path, and the like.

  As described above, the width and depth of the microchannel are about several μm to several hundred μm. For this reason, the amount of product produced in one microchannel is about several tens of μl per minute. However, when chemically synthesizing using a microchannel or when industrially producing fine particles, the microchannel It is generally said that it can be realized by increasing the degree of integration of microchannels formed on a substrate or by three-dimensionally stacking microchannel substrates having integrated microchannels. Is sometimes referred to as numbering up of microchannels.

  However, this microchannel integration technique has only been reported in the past as an example in which several microchannel substrates having one microchannel are stacked in a model manner (for example, Patent Documents). 4, see Non-Patent Document 3), practically arranging several tens to several hundreds of microchannels in a plane, and uniformly feeding fluid to all the microchannels; It has not been studied yet to produce a uniform droplet by arranging three to several tens of microchannel substrates having microchannels integrated from ten to several hundreds. Industrial mass production by has been very difficult in the past.

  From the background as described above, it has been eagerly desired to produce uniform droplets with an industrial apparatus accompanying the improvement of the planar integration degree and the three-dimensional integration degree of the microchannels.

JP 2003-225900 A International Publication WO02 / 068104 Pamphlet Japanese Patent No. 3511238 JP 2002-292275 A H. Hisamoto et. al. (H. Hisamoto et al.), "Fast and high conversion phase- transfer synthesis synthesis the liquid-liquid interface formed in a microchannel chip." Commun. , 2662-2663, 2001 Takashi Nishisako et al., “Liquid microdroplet generation in microchannels”, Proceedings of the 4th Chemistry and Microsystem Study Group, 59 pages, 2001 Kikutani et al., “High-yield microchannel synthesis using pile-up microreactors”, Proceedings of the 3rd Chemistry and Microsystem Research Meeting, 9 pages, 2001

  The microchannel structure used for the production of gels and microcapsules with uniform particle diameters is formed on a substrate of glass, silicon, metal, resin, etc. by photolithography and etching using a photomask. A flow path is formed by joining a groove substrate formed with a groove by a cutting process and a cover body with holes, but this method has low productivity and is difficult to mass-produce. In addition, there is a problem that the liquid tends to elute from the flow path formed by covering, and the lid needs to be joined to a plate formed with a groove by heat welding or adhesive. Further, when this was used as a manufacturing apparatus for generating droplets, the productivity was remarkably low.

  The present invention has been made in view of the above problems, and a microchannel structure manufactured in a large quantity at a low cost is obtained by improving the degree of integration of microchannels in a three-dimensional manner so that fluids are uniformly distributed in all microchannels. It is an object of the present invention to provide a production apparatus for producing droplets capable of feeding a liquid and producing a large amount of products.

  In order to solve the above-mentioned object, the present invention manufactures a mold (stamper) of a micro flow channel structure, and molds / reproduces a large number of resin substrates having a specific hardness using the mold. This is a micro-channel assembly device for generating droplets that is used by sandwiching the micro-channel structure substrate between a jig harder than the channel-structure substrate and a plate softer than the micro-channel structure substrate. MR block "). The MR block includes an introduction port for introducing a dispersed phase and an introduction channel communicating with the microchannel substrate, an introduction port for introducing a continuous phase and an introduction channel communicating with the microchannel substrate, A discharge channel and a discharge port for discharging particles generated through the phase and the continuous phase are provided.

  That is, the present invention is a microchannel structure having a fluid inlet for introducing a fluid, a microchannel for generating microparticles by the fluid, and a fluid outlet for discharging a fluid containing the generated microparticles, The microchannel structure includes a fluid supply structure that supplies the fluid to the microchannel, a microchannel substrate having the microchannel, and a plate interposed therebetween, Formation of droplets in which the base material of the flow path substrate is a resin having a durometer hardness test method compliant with JIS K 6253 and having a hardness of 70 or higher in Type D and a molding shrinkage rate compliant with JIS K 7152-4 of 3% or less This is a microchannel assembly device for use.

  Further, the present invention provides a microchannel structure having an inlet for introducing a dispersed phase and a dispersed phase introducing microchannel that communicates with the inlet, an inlet for introducing a continuous phase, and a continuous phase introduction microchannel that communicates with the inlet, A liquid containing a droplet generating microchannel formed of a dispersed phase by intersecting the dispersed phase introducing microchannel and the continuous phase introducing microchannel, and a fine particle generated through communication with the droplet generating microchannel And a fluid flow outlet for discharging the liquid droplets.

  In the present invention, plates having a hardness of 70 or less according to JIS K 6253 according to JIS K 6253 are disposed on both sides of the microchannel substrate, and the plates of the microchannel substrate are harder on both sides thereof. It is the above-described micro-channel assembly device for droplet generation having a laminated body in which a jig is arranged.

  Further, according to the present invention, the fluid supply structure has one or more through holes as fluid inlets for introducing the fluid, and stores the introduced fluid in communication with the fluid inlets temporarily. One or more for supplying fluid from the storage space to each fluid inlet of each of the one or more microchannels formed in the microchannel substrate from the storage space. It has a supply channel formed radially and / or curvilinearly, and at least one of the fluid supply structures is the above-described droplet generating microposition located below the upper and lower jigs to be crimped It is a flow path assembly device.

  In the present invention, the microchannel substrate and the cover body that forms a channel by covering the channel formed on the microchannel substrate are brought into close contact with each other by crimping a flat jig. The above-described micro-channel assembly device for generating droplets.

  The present invention is also the above-described micro-channel assembly device for generating droplets, wherein the cover body is harder than the channel substrate.

Further, in the present invention, the flow path formed in the micro flow path substrate has a line or point symmetric configuration in the micro flow path substrate, and there is a space at the center of symmetry, and the crimping jig is provided. The present invention also relates to the above-described microchannel assembly for generating a droplet having a positioning mechanism for three-dimensionally stacking microchannel substrates or installing a cover body. is there.

  The present invention also provides the above-described micro-channel assembly device for generating droplets, wherein a part of the micro-channel substrate has a recess for inserting a jig for separating the micro-channel substrates from the stacked state.

  In the present invention, the holes communicating with the plate interposed between the fluid supply structure and the microchannel substrate are arranged on the plane of the crimping jig, and the fluid supply structure, the microchannel substrate, It is the above-mentioned micro-channel assembly device for generating droplets having a structure in which holes provided in a plate interposed between them are aligned at a time.

  The present invention is also the above-described micro-channel assembly device for generating droplets in which a plate interposed between the fluid supply structure and the micro-channel substrate is smaller than the micro-channel substrate.

  In addition, the present invention is the above-described micro-channel assembly device for generating droplets, in which a channel surface formed on a micro-channel substrate is subjected to a hydrophilic treatment.

  In the present invention, when the microchannel substrates are stacked, the channel formed on the microchannel substrate is covered with the back surface of the channel forming surface of the microchannel substrate different from the microchannel substrate. The droplet generating micro-channel assembly device is formed by laminating as a cover body to be used.

  In addition, the present invention provides the above-described liquid in which the depth of the introduction channel of the introduction microchannel from the vertical hole channel (through hole) communicating with the fluid supply structure is deeper than the depth of the droplet generation microchannel. This is a droplet generating microchannel assembly device.

  The present invention is also the above-described micro-channel assembly device for generating droplets, wherein a part of the channel is deep in the discharge channel formed on the micro-channel substrate.

  In the present invention, when two microchannel substrates having different channel structures are opposed to each other on the surfaces where the channels are formed, the channels on the microchannel substrates having different channels are The above-described micro-channel assembly device for generating droplets has a structure in which droplets are formed at intersecting portions.

  Further, the present invention is the above-described micro-channel assembly device for generating droplets, wherein the channel structure on one micro-channel substrate is annular and does not have a start point and an end point.

  The present invention also provides the above-described micro-channel assembly device for generating droplets, wherein the grooves of one channel substrate are concentric circles.

  Further, the present invention provides the above-described droplet generation in which the flow path to be introduced into the dispersed flow path or continuous phase micro flow path substrate is positioned below the flow path substrate, and the liquid feeding direction discharged through each substrate is upward. This is a microchannel assembly device for use.

  Hereinafter, the present invention will be described in detail.

  As described above, the present invention includes a fluid introduction port for introducing a fluid, a micro flow channel that generates fine particles by the introduced fluid, and a fluid discharge port that discharges a fluid containing the fine particles generated in the micro flow channel. It is the microchannel structure which has. This microchannel structure is composed of a fluid supply structure for supplying fluid to the microchannel and a microchannel substrate having a microchannel. Furthermore, as a base material of the microchannel substrate, a resin having a hardness of 70 or higher in Type D by a durometer hardness test method in accordance with JIS K 6253 and a molding shrinkage rate in accordance with JIS K 7152-4 of 3% or less. This is a microchannel assembly device for droplet generation.

  For this reason, the present invention produces a mold (stamper) of the above-described microchannel structure. The stamper may be a micro-channel substrate (groove substrate) formed by forming a convex portion on a plate that can be finely processed using a mechanical technique, and transferring it by a chemical or mechanical method. A metal stamper obtained by copying the groove mold by electroforming or the like may be transferred to the resin on the plate on which the groove is formed. A known technique such as injection molding may be used for the transfer.

  Thermosetting resin such as POM (polyacetal) nylon, polyetherimide, polyethylene, polyphenylene sulfide, polypropylene, polyethylene, melamine, phenol, epoxy, etc. The resin may be appropriately selected in consideration of ease of moldability, liquid resistance of a liquid that becomes a continuous phase or a dispersed phase, and ease of surface modification. Specifically, in order to achieve the object of the present invention as a micro-flow channel assembly device after molding, such as injection molding, a durometer hardness test method compliant with JIS K 6253 is used. A resin having a hardness of 70 or more in Type D and a molding shrinkage rate of 3% or less in accordance with JIS K 7152-4 can be used.

  The fluid supply structure for supplying fluid to the microchannels used in the droplet generating microchannel assembly apparatus of the present invention can also be manufactured using a known method in the same manner as the microchannel substrate. . The fluid supply structure has one or more through holes as fluid inlets for introducing fluid, has a storage space for temporarily storing fluid introduced through communication with the fluid inlets, and One or more radially linear and / or curved lines for supplying fluid from the storage space to each fluid inlet of each of the one or more microchannels formed in the microchannel substrate. And at least one of the fluid supply structures is positioned below the space between the upper and lower jigs to be crimped, so that the microchannel assembly for generating droplets according to the present invention is provided. It becomes the structure of the body device.

  The substrate thus replicated (microchannel substrate) and the cover body that forms a channel by covering the channels formed on the microchannel substrate are made of a jig that is harder than this substrate and a plate that is softer than the substrate (hereinafter referred to as the substrate). "Packing"), and more specifically, by using a durometer hardness test method sandwiched between plates having a hardness of 70 or less in Type D, for example, Teflon (registered trademark), the cover body and the microchannel substrate (Groove substrate) forms the flow path, but in order to crimp the entire groove flow path, the packing needs to be located in the entire flow path range, particularly in the part located directly above and below the flow path, that is, on both sides. is there.

  Further, a laminated body in which a jig harder than the base material of the microchannel substrate, for example, a flat plate-shaped stainless steel jig, is arranged on both sides, and the cover body and the microchannel substrate (groove substrate) are pressed and adhered. Can be used for the droplet generating microchannel assembly device of the present invention. Moreover, it is preferable that the cover body be a base material harder than the microchannel substrate.

  The MR block includes an inlet for introducing a dispersed phase and a dispersed phase introducing microchannel communicating with the inlet, an inlet for introducing a continuous phase and a continuous phase introducing microchannel communicating with the inlet, and a dispersed phase introducing microchannel. And a continuous-phase-introducing microchannel intersecting the droplet-generating microchannel consisting of a dispersed phase, and a fluid discharge port for discharging a fluid containing fine particles generated in communication with the droplet-generating microchannel Provided with an introduction port for introducing a dispersed phase or a continuous phase and at least one distribution channel for evenly feeding to an introduction channel communicating three-dimensionally to a microchannel substrate, a dispersed phase and a continuous phase By using a structure in which there are no flow paths for collecting the discharge flow paths for discharging the particles generated via the crimping jig, the micro flow path substrate can be evenly crimped. .

In addition, the surface of the flow path formed on the micro flow path substrate to be pressure-bonded is preferably subjected to a hydrophilic treatment, and the hydrophilization treatment can more easily generate droplets using the aqueous phase as a continuous phase. For surface treatment, gold sputtering, nickel sputtering, thin film method such as silicon or SiO ₂ sputtering, ashing treatment, plasma treatment, surface modification of hydrophilic groups such as ozone treatment and sulfonation, reverse sputtering, acid / alkali treatment What is necessary is just to select arbitrarily from the hydrophilization method etc. by the surface area increase by these.

  When laminating the microchannel substrate, the channel formed on the microchannel substrate is made hydrophilic on the back surface, preferably the surface of the microchannel substrate that is different from the microchannel substrate. It is also possible to stack without separately providing a cover body by stacking the treated back surface as a cover body.

  In addition, if the jig is crimped using bolts or vise parts only at the periphery of the substrate in order to bring the flow path into close contact with the cover body, the adhesion of the inner peripheral flow path will deteriorate and leak from the flow path. This makes it difficult to produce uniform droplets, so that the inner periphery can be prevented from leaking by tightening with bolts etc. through the hole in the center of the substrate, etc. The convexity between the force points, for example, in the disc channel, the region where the channel and the cover body are in close contact with each other has a conical shape, thereby improving the adhesion of the channel.

  In this way, the resin substrate on which the groove is formed and the lid are brought into close contact with each other by pressure bonding, but the duplicated substrate has a thickness unevenness of about several μ to several tens of microns. Therefore, it is necessary to press-bond with a force below the range of plastic deformation of the substrate to be used. That is, the higher the planarity of the substrate, the closer the groove substrate and the cover body can be brought into contact with each other with a low pressure. In addition, if the pressure is still pressed, the groove substrate will cause creep deformation even with a force within the elastic deformation range, so it is desirable that the groove and the cover be in close contact with each other and the pressure should be as low as possible without causing fluid to leak from the groove. . Also, the portion that contacts the groove of the cover body is convex on the groove side due to the crimping force, narrows the flow path, makes the feeding of the introduced liquid non-uniform, and may cause breakup of the generated droplets. The lid substrate is preferably the same as the flow path substrate or harder than the groove substrate.

  In addition, since crimping causes creep deformation of the substrate over a long period of time, it is desirable that the crimping force be appropriately reduced and stored when not in use.

  Further, in consideration of flow path deformation due to pressure bonding, the depth of the flow path of the introduction micro flow path from the vertical hole flow path (through hole) communicating with the fluid supply structure is determined by the micro flow path for droplet generation. By making it deeper than the depth, it is possible to supply a continuous phase and a dispersed phase fluid uniformly to each of the accumulated droplet generation channels and to make the droplets that change due to the shearing force of the fluid uniform over a long period of time. Become.

  In addition, when the discharge channel is narrow, there is a phenomenon that the generated droplets break up when the droplets are crushed. In the discharge channel formed on the microchannel substrate, a part of the channel is deepened from the middle. This makes it possible to reduce this splitting phenomenon due to flow path deformation over a long period of time.

  The apparatus of the present invention also has a positioning mechanism for three-dimensionally stacking the microchannel substrates or installing the cover body. That is, when laminating a micro-channel substrate (groove substrate) or matching with a substrate functioning as a cover body, it is particularly necessary when using a non-transparent substrate, but it is necessary to have a positioning mechanism. is there. Here, the positioning mechanism refers to a structure having one or more orientation flat portions or irregularities at the time of molding, a center hole formed by a cut punch after injection filling with resin at the time of injection molding, and a flow path formation at the time of molding. A hole or the like formed in accordance with a reference position such as a registration mark. Using this positioning mechanism, the cover body and the groove substrate can be easily positioned and stacked three-dimensionally.

  Further, by providing a structure in which a notch is provided in a part of the peripheral portion of the substrate, when disassembling and cleaning the MR block, a jig is inserted into this gap to facilitate disassembly of the flow path substrate and the cover body. .

  In addition, the flow path formed in the micro flow path substrate has a line or point symmetric configuration in the micro flow path substrate, has a space at the center of symmetry, and has a crimping jig. By using a structure in which holes communicating with the packing of the crimping jig are arranged on a flat surface, the flow path substrate is crimped and a plurality of holes provided on a single flat packing can be used at a plurality of locations on the flow path substrate. The supply port and the discharge port can be aligned at a time. At the same time, the groove substrate and the cover body can be pressure-bonded. In other words, the holes communicating with the plate interposed between the fluid supply structure and the microchannel substrate are arranged on the plane of the crimping jig, and between the fluid supply structure and the microchannel substrate. It becomes a structure which aligns each hole at once with the hole provided in the board interposed. At this time, there may be a structure in which a convex partition lower than the thickness of the packing is provided in a portion of the crimping jig in contact with the packing to prevent liquid leakage and mixing in this portion.

  Furthermore, by making a hole in a portion of the packing provided between the crimping jig and the micro-channel substrate that is substantially unnecessary for channel crimping, the channel range (area) to be crimped is narrowed and the total press pressure is reduced. The flow path can be arranged avoiding the portion where the thickness unevenness of the substrate becomes large, or the space for the crimping mechanism and the space for the positioning mechanism.

  Moreover, it is preferable that the plate interposed between the fluid supply structure and the microchannel substrate is smaller than the microchannel substrate. By adopting such a configuration, for example, when a disk-shaped circular substrate is used, it is possible to remove the inner and outer peripheries where unevenness in thickness is likely to occur, and even if the flatness of the inner and outer peripheral portions is in contact with the packing Therefore, it is not necessary to apply an extra crimping force, and only the portions that need to be in close contact can be in close contact.

In addition, when two microchannel substrates having different channel structures face each other on the surfaces where the channels are formed, portions where the channels on the microchannel substrates having different channels intersect By selecting a microchannel substrate that has two channels depth, crossing angle, number of disperse phase channels communicating with one continuous phase, etc. Thus, the structure of the intersection can be easily controlled, and the droplet generation amount and particle size can be controlled.
For example, in order to reduce the pressure loss of each flow path, a continuous phase or a disperse phase introduction micro flow path and a discharge flow path having a large cross-sectional area are formed on one side, and the other micro flow path serving as a cover body When 10 disperse flow channels having a width and depth smaller than the continuous phase are formed on the substrate and face each other, the intersection has a structure in which a large number of fine disperse phase channels communicate with the thick continuous phase. . Further, by selecting and facing a cover body having 20 fine dispersed-phase flow paths, it is possible to easily select and combine such a structure that doubles the amount of the dispersed phase fed to the continuous phase.

  In addition, the channel structure on one microchannel substrate is annular or specially concentric and does not have a start point and an end point, thereby facilitating hole position formation or crimp positioning. I can do it. For example, in the case of a ring shape like the cover body 44 in FIG. 22A, it is necessary to form a through hole in the substrate in order to stack the flow paths, but the other micro flow path substrate 45 facing each other By forming the hole positions at fixed radial positions at equal intervals, the hole positions provided on the substrate of the cover body 44 may be formed at the same radial positions as the substrate 45 at equal intervals. The structure does not need to be precisely controlled, and hole formation is possible easily and inexpensively. The through hole of the flow path substrate may be formed integrally with the mold holder, or may be mechanically processed after the substrate is formed. In addition, the flow path to be introduced into the dispersed phase or continuous phase micro flow path substrate is positioned below the flow path substrate, and the liquid feeding direction of the fluid discharged through each substrate is upward, so that the liquid inside the MR block It is possible to easily replace the air in the liquid feeding section with the liquid feeding fluid. Further, the positions of the introduction and discharge ports may be adjusted according to the specific gravity of the liquid feeding liquid.

  The apparatus of the present invention has a recess for inserting a jig to separate the microchannel substrates from the stacked state in a part of the microchannel substrates when a plurality of microchannel substrates are stacked. The microchannel substrates stacked in this manner can be efficiently separated.

  Examples of uses in the production method using the MR block of the present invention include a high-performance liquid chromatography column filler, pressure measurement film, carbonless (pressure-sensitive copying) paper, toner, thermal expansion agent, heat medium, and control. Light glass, gap agent (spacer), thermochromic (thermosensitive liquid crystal, thermosensitive dye), magnetophoresis capsule, pesticide, artificial feed, artificial seed, fragrance, massage cream, lipstick, vitamins capsule, activated carbon, enzyme-containing capsule And DDS (drug delivery system).

  Hereinafter, the MR block of the present invention will be described more specifically with reference to the drawings.

  A typical conceptual diagram of the MR block in the present invention is shown in FIG. The MR block is formed by a hydrophilic treatment on the surface of the groove substrate (15) formed by copying and replicating a large number of resin-made circular substrates, then positioned and laminated together with the cover body (16), and is softer than the groove substrate and the cover body. The substrate is positioned and sandwiched between the packings (6) with the positioning pins (5) or the like without welding or bonding, and the outer peripheral bolt (3) and the position opened in the center of the substrate are tightened with the bolt (4). A substrate in which one or more layers of the groove substrate and the cover body are laminated is crimped by the upper and lower crimping jigs (1, 2), and the substrates are brought into close contact with each other. In addition, there are supply channels (9, 10) connected to the through holes (7, 8) for distributing and supplying the fluid to each substrate and each channel above and below the crimping jig. 3 is a radial fluid supply channel as shown in FIG. 3, and has a structure in which a continuous phase and a dispersed phase can be introduced from the inlets (11, 12) to each channel substrate. Further, it has a flow path (13) for collecting and discharging the generated liquid droplets from the respective flow path substrates and flow paths.

  FIG. 4 shows a laminated example of the MR block substrate in the present invention. FIG. 4 shows that five layers are stacked on the upper side through positioning pins previously opened with the groove substrate (15) facing upward on the lower packing (6), the cover body (16) is placed, and the upper packing (6) is placed thereon. This is an example in which is located. The packing is not provided individually such as an O-ring, and a through hole is formed at a necessary position in one flat plate and a groove substrate is crimped. However, a substantially unnecessary central portion is a space, and the groove substrate It is a crimping mechanism through the crimping bolt along with the shaft positioning. Further, the cover body and the packing are easily positioned and stacked by the groove substrate and positioning pins. In addition, the continuous phase and the dispersion tank are introduced from below, but by doing so, it is possible to separate only the crimping jig and the groove substrate part from the liquid supply / discharge part and to remove the cartridge type. .

  5, FIG. 6, and FIG. 7 show the cross sections of the substrate and packing of FIG. FIG. 4 (a) shows a channel formed with the grooves facing upwards of the substrate (15) and the groove covers facing the back surfaces of the groove substrates located above, respectively. (16) is used as a flow path, and a cross-section of each micro flow path and each supply flow path of each substrate when supplying a continuous phase and a dispersed phase from below and discharging droplets upward is shown.

  FIG. 4B is an enlarged view of a part of the stacked substrate in the right part of FIG. In FIG. 4B, AA ′, BB ′, and CC ′ are respectively a cross-sectional view of a groove portion that forms a flow path, and a cross-sectional view of continuous phase and dispersed phase supply flow paths (7, 9). FIG. 10 is a cross-sectional view of the discharge channel (19). Here, the supply channel may pass through the packing and cover body to reach the jig, but it is shown in the figure in consideration of removing bubbles in the channel before use and cleaning after use. As described above, it is preferable that the dead end is not provided and the supply channel is not formed.

  FIG. 8 (a) shows a groove substrate (continuous phase on the bottom surface and a disperse phase groove on the top surface on the groove substrate (21) formed on one side of the substrate with the continuous phase introduction and discharge channels and the substrate ( 22) and a cover body (23) in which a groove of a dispersed phase is formed on the lower surface are positioned and faced to form a structure (24) intersecting at one point of each layer and each microchannel, and packing (6) vertically The structure where is located is shown. The MR block shown in FIG. 8B may be obtained by sandwiching this with a crimping jig similar to that shown in FIG. Further, if the substrate is provided with a notch (26) and the notch shown in FIG. 8C is arranged, the laminated substrate can be easily disassembled for the purpose of cleaning or the like.

FIG. 9 shows a continuous phase introduction channel, a discharge channel, and a part of the organic phase introduction channel arranged in the radial direction on the groove substrate (29) formed on one side of the substrate and the dispersed phase on the lower side. A ring-shaped groove that is a part of the groove, a groove substrate (28) in which a flow path similar to (29) is formed on the upper surface, and a cover body (28) in which a ring-shaped groove similar to the lower surface is formed on the lower surface ( 27) shows a structure (31) in which only the center position is positioned and faced to form a structure (30) intersecting at several points of each layer and each microchannel, and packing (6) is positioned above and below. This is sandwiched by a crimping jig similar to that in FIG. 1 and crimped so that the flow path is brought into close contact (shown in FIG. 9B), but the through holes that form the supply / discharge flow path formed in the cover body have the same radial position. In this case, it may be formed at any position as long as the groove substrate and the supply flow path can be connected, and can be laminated without the need for precise alignment between the surfaces. In this way, it is possible to obtain an MR block with a simple three-dimensional integration of substrates. At this time, the dispersed phase flowing in a ring shape preferably has a narrower groove than the continuous phase groove, and considering the liquid feeding resistance (pressure loss), the substrate can be effectively used by setting the liquid feeding direction from the inner circumference side to the outer circumference. You can use the surface. FIG. 10A shows a flow path substrate formed by stacking, and FIG. The continuous phase and the dispersed phase are introduced from the supply flow path (33, 32), intersect the number of rings formed by 34, form droplets, and are discharged to the 35 discharge flow path. By doing so, since the layers can be stacked without positioning in the circumferential direction, a simple structure can be obtained in the case of three-dimensional integration.
In contrast, FIG. 7 requires precise positioning and is not convenient when stacking a large number.

  In the MR block of the present invention, a mold (stamper) of a micro flow channel structure is manufactured, and a number of resin substrates are molded and duplicated using the mold, a jig harder than the micro flow channel structure substrate, A micro-channel assembly device for generating droplets having a structure in which the micro-channel structure substrate is sandwiched between plates softer than the channel structure substrate, wherein the channel is bonded without being welded or bonded. Thus, one or more layers of the groove substrate whose surface is subjected to hydrophilic treatment and the cover body are laminated and brought into close contact with each other, and uniform droplets can be used over a long period of time to produce fine particles.

  Hereinafter, examples of the present invention will be described, and the embodiments of the invention will be described in more detail. It is needless to say that the present invention is not limited to the following examples and can be arbitrarily changed without departing from the gist of the present invention.

Example 1
The conceptual diagram of the Example in this invention was shown in FIG. A chromium and gold film is formed on a glass master, and a nickel plating is formed thereon to a thickness of 300 μm and peeled off from the master to form a stamper. By injection molding, Duracon (registered trademark) M90-44 grade resin is used. A substrate was manufactured by injection molding and transferring a groove flow path having a depth of 130 μm and a width of 310 μm on one side of a φ135 mm substrate at the intersection. The inlet and outlet are formed with a φ1.5 mm drill, and injection is performed on three polyacetal resin groove substrates (15) formed by sputtering 20 nm of chromium and 100 nm of gold on both surfaces of the substrate. Formed in the same manner as the polyacetal resin cover body (16) obtained by molding, positioned and aligned, and sandwiched with Teflon (registered trademark) packing up and down, which is the upper crimping jig as shown in the figure The flow path was brought into close contact by pressing the 12 outer peripheral bolts of the outer periphery with 20 N · m, sandwiched by a stainless steel crimping jig having a convex center (outer periphery-inner peripheral height difference 130 μm). In addition, an MR block is formed by disposing a continuous flow channel portion and a disperse flow channel portion of a radial groove having a width of 1.0 mm and a depth of 1.0 mm on the crimping jig, and on the discharge flow path collecting portion and the jig, respectively. It was.

  FIG. 12 (a) shows the microchannel substrate (36) used and FIG. 12 (b) in which a part of the microchannel is enlarged. As shown in FIG. 12, one microchannel has an inlet (32) for introducing the first fluid, an inlet (33) for introducing the second fluid, a junction (34), and an outlet (35). The unit is configured as one unit. As shown in FIG. 12, 100 microchannels of one unit are formed on a microchannel substrate. A flare-fit adapter for introducing fluid (11, 12) to be introduced was connected to the MR block, and connected to a fluid feeding pump (17, 18) via a Teflon (registered trademark) tube. The microdroplets generated in the microchannels were discharged and collected through the flare fit adapter to the fluid outlet (14) from the upper discharge channel assembly. Here, as the Teflon (registered trademark) tube, it was confirmed that the discharged droplets were not broken, and a Teflon (registered trademark) tube having an inner diameter of 2 mm was used.

In this embodiment, a 3% polyvinyl alcohol aqueous solution is fed to the microchannel structure at a feed rate of about 6.0 ml / min by the first fluid feed pump (17), and the second fluid feed pump is supplied. A liquid pump (18) feeds a mixed solution of divinylbenzene and butyl acetate to the microchannel structure at a rate of about 2.0 ml / min, and each inlet port (11 , 12) from the storage space (39, 42) and the radial supply channel (40, 43) to the supply channel (7, 8), which is a vertical hole communicating with each channel.
Each channel is introduced into each of the Y-shaped microchannels formed on the microchannel substrate (15) through the supply channels (7, 8) stacked on the three-dimensional droplets, and the Y-shaped A microdroplet is generated by shearing a mixed solution of divinylbenzene and butyl acetate, which is a dispersed phase, with a polyvinyl alcohol aqueous solution, which is a continuous phase, at a confluence portion of the microchannel. The fluid containing microdroplets collected from the flare fit adapter connected through the discharge flow path from the discharge port (35) and connected to the fluid discharge port (14) was sampled, and 100 microdroplets were measured. As a result, the average particle size was about 257 μm and the particle size dispersion was 3.8%. FIG. 13 shows a photograph of the collected droplets. From this result, by using the MR block of the present invention, the substrates can be stacked without welding or bonding. A uniform droplet can be obtained by pressure bonding. The particle size dispersion is a value obtained by dividing the standard deviation of the sampled fine droplets by the average particle size, and is a numerical value indicating the spread of the particle size distribution.

(Comparative Example 1)
A conceptual diagram of the comparative example is shown in FIG. In the same manner as in Example 1, a polyacetal resin cover body (16) obtained by injection molding in the same manner on the three polyacetal resin groove substrates (15) and sputtering chromium and gold on the surface was positioned. In addition, this was sandwiched between flat crimping jigs as shown in the figure, and 12 outer peripheral bolts were crimped at 24 N · m.

  A 3% polyvinyl alcohol aqueous solution is fed to the MR block by a first fluid feed pump (17) at a feed rate of about 6.0 ml / min. The mixed solution of divinylbenzene and butyl acetate is fed to the microchannel structure at a rate of about 2.0 ml / min by the pump (18), and the fluid containing the ejected microdroplets is sampled to obtain 100 microdroplets. As a result, the average particle size was about 160 μm and the particle size dispersion degree was 50%. FIG. 15 shows a photograph of the collected droplets. In order to confirm the degree of adhesion of the substrates, pressure sensitive paper was inserted between the flow path substrates, and bolts were similarly tightened to confirm the result as shown in FIG. FIG. 16 shows a portion where the black portion is in close contact.

(Example 2)
On the same three polyacetal resin groove substrates (15) as in Example 1, a polyacetal resin cover body (16) obtained by injection molding in the same manner and sputtering chrome and gold on the surface was positioned. The two outer peripheral bolts on the outer periphery were clamped with a torque of 24 N · m and one central bolt with a torque of 40 N · m.

  A 3% polyvinyl alcohol aqueous solution is fed to the MR block by a first fluid feed pump (17) at a feed rate of about 6.0 ml / min. The mixed solution of divinylbenzene and butyl acetate is fed to the microchannel structure at a rate of about 2.0 ml / min by the pump (18), and the fluid containing the ejected microdroplets is sampled to obtain 100 microdroplets. As a result, the average particle size was about 243 μm and the particle size dispersion degree was 5.2%. From this result, by using the MR block of the present invention, the substrates can be stacked without welding or bonding. A uniform droplet can be obtained by pressure bonding. Further, when pressure sensitive paper was inserted between similar flow path substrates and bolts were similarly tightened, the results were uniform as shown in FIG. 17, and the in-plane pressure was 1.8 to 2.5 MPa.

(Example 3)
Using MR blocks similar to those of Example 2, the depth of the groove at the intersection is 45 Φ120 mm Delrin (registered trademark) DS500M grade resin groove substrate having a groove with a depth of 50 μm and a width of 120 μm ( 15) and a cover body, and a groove substrate obtained by plasma-treating the entire surface as a flow path in an argon gas atmosphere has 12 outer peripheral bolts of 19 N · m and one central bolt of 34 N · m torque. Crimped with. The first fluid feed pump (17) feeds a 4% polyvinyl alcohol aqueous solution to the microchannel structure at a feed rate of about 20 ml / min, and the second fluid feed pump (18). As a result of feeding a mixed solution of divinylbenzene and butyl acetate to the microchannel structure at about 10 ml / min, sampling the fluid containing the ejected microdroplets and measuring 100 microdroplets, the average particle size Was about 95 μm and the particle size dispersion degree was 8.0%. From this result, by using the MR block of the present invention, uniform droplets can be obtained by highly laminating and pressing the substrates without welding or bonding.

Example 4
A photograph of the channel of the groove substrate used in Example 4 is shown in FIG. 18 (a), and an enlarged view of the channel is shown in FIG. 18 (b). Using Duracon (registered trademark) M270S grade resin, the groove groove depth of 50 μm and width of 120 μm at the intersection is transferred to the Φ135 mm substrate and the groove flow path is deepened by 200 μm from the middle. The groove substrate formed integrally with the surface of the cover body and the surface of the cover body were plasma-treated in an argon gas atmosphere, and pressure-bonded in the same manner as in Example 2 to obtain an MR block. A 4% polyvinyl alcohol aqueous solution is fed to the block at a liquid feed rate of about 1.2 ml / min through the first fluid feed pump (17) to the second fluid feed pump. A pump (18) is used to send a mixed solution of divinylbenzene and butyl acetate to the microchannel structure at a rate of about 0.4 ml / min, and the fluid containing the discharged microdroplets is sampled to obtain 100 microdroplets. As a result of measurement, the average particle size was about 95 μm and the particle size dispersion degree was 6.7%. Further, after the MR block was continuously operated for 500 hours, the collected droplets had an average particle size of about 91 μm and a particle size dispersion of 12%. This is due to a slight increase in fine particles. Also, the MR block is disassembled, and a new lid substrate is again positioned between the groove substrate using each groove flow path, and the grooves and the lid substrate are alternately laminated and crimped as shown in FIG. As a result of assembling and feeding the block, the liquid droplets were good with an average particle size of 90 μm and a particle size dispersion of 6.0%.

(Example 5)
Using a groove substrate equivalent to that in Example 4, a stainless steel lid substrate having a thickness of 100 μm is positioned between each groove substrate, and the grooves and the lid substrate are alternately laminated and pressure-bonded as shown in FIG. As a result of assembling the block and feeding the liquid, it was a good droplet with an average particle size of 94 μm and a particle size dispersion of 5.8%.

(Example 6)
A schematic view of the flow path of the groove substrate used in Example 6 is shown in FIG. 20 (a), and the substrate in which the groove substrate (39) and the cover body (40) face each other is shown in FIGS. 20 (b) and 20 (b). An enlarged view of the flow path in the substrate is shown in FIG. A groove substrate (38) formed using a Delrin (registered trademark) DS500M grade resin groove substrate with a water phase and a part of an organic phase having a flow path depth of 45 μm, and a part of the organic phase intersecting the water phase flow path On the surface of the cover substrate (39) having a flow path formed at a depth of 7 μm, 20 nm of chromium and 100 nm of gold are sputtered, and each surface is precisely positioned and pressure-bonded so as to face each other. did. The substrate (40) has a structure in which 50 flow paths intersecting 40 continuous phases are integrated with respect to one continuous phase. A 4% polyvinyl alcohol aqueous solution is fed to this block by a first fluid feed pump (17) at a rate of about 0.5 ml / min. The pump (18) is used to send a mixed solution of divinylbenzene and butyl acetate to the microchannel structure at a rate of about 0.25 ml / min. The fluid containing the ejected microdroplets is sampled to obtain 100 microdroplets. As a result of measurement, the average particle size was 29.8 μm, and the particle size dispersion degree was 11.9%. As described above, even fine droplets having a high degree of dispersion were obtained.

(Example 7)
A schematic view of the channel of the groove substrate used in Example 7 is shown in FIG. Using a Delrin (registered trademark) DS500M grade resin grooved substrate, a water phase and a part of the organic phase have a flow path depth of 45 μm, and a depth of 7 μm at the portion where the organic phase of the water phase merges with two stages of flow. The surface of the substrate (41) on which the road substrate is formed is subjected to plasma treatment in an argon gas atmosphere, and is similarly positioned and faced with a cover body having only a through-hole that does not have a surface-treated flow path to form an MR block. . A 4% polyvinyl alcohol aqueous solution is fed to the block at a liquid feed rate of about 0.2 ml / min through the first fluid feed pump (17) to the second fluid feed pump. A pump (18) is used to send a mixed solution of divinylbenzene and butyl acetate to the microchannel structure at a rate of about 0.1 ml / min, and the fluid containing the ejected microdroplets is sampled to obtain 100 microdroplets. As a result of measurement, the average particle size was 28.2 μm, and the particle size dispersion degree was 12.9%. As described above, even fine droplets having a high degree of dispersion were obtained.

(Example 8)
A schematic view of the flow path of the groove substrate used in Example 8 is shown in FIG. 22 (a), and the substrate in which the groove substrate (45) and the cover body (44) face each other is shown in FIGS. 22 (b) and 22 (b). An enlarged view of the flow path in the substrate is shown in FIG. Using a Delrin (registered trademark) DS500M grade resin groove substrate, an aqueous phase and a part of an organic phase are formed on one substrate (45) with a flow path depth of 45 μm, and a ring shape with a depth of 7 μm is formed on the other substrate. A hole is machined at a predetermined radial position without determining the circumferential position of the substrate on which a part (44) of the organic phase flow path is formed, and the surface of the substrate is plasma-treated in an argon gas atmosphere. The MR surfaces were made by positioning the groove surfaces with the center hole. A 4% polyvinyl alcohol aqueous solution is fed to the block at a rate of about 1.0 ml / min through the first fluid feed pump (17) to the microchannel structure, and the second fluid feed pump is used. A pump (18) is used to send a mixed solution of divinylbenzene and butyl acetate to the microchannel structure at a rate of about 0.5 ml / min, and the fluid containing the ejected microdroplets is sampled to obtain 100 microdroplets. As a result of measurement, the average particle size was 30.1 μm, and the particle size dispersion degree was 11.1%. Thus, the hole processing and lamination could be simplified.

Example 9
An enlarged view of the channel of the groove substrate used in Example 9 (before through hole processing) is shown in FIG. 23 (a), and an enlarged view of the continuous phase introduction part in FIG. 23 (a) is shown in FIG. 23 (b). FIG. 23 (c) shows an enlarged view of the discharge channel in FIG. 23 (a). Using a Delrin (registered trademark) DS500M grade resin, the groove channel depth is 110 μm, the width is 240 μm, and the channel on the discharge side is deeper by 50 μm than the intersection so that the channel on the discharge side is 160 μm from the middle. The through hole was post-processed while being transferred to a Φ120 mm substrate. The three groove substrates and the surface of the cover body were subjected to plasma treatment in an argon gas atmosphere and pressure-bonded in the same manner as in Example 2 to obtain an MR block. A 3% polyvinyl alcohol aqueous solution is fed to this block by a first fluid feed pump (17) at a feed rate of about 3.6 ml / min. A pump (18) is used to feed a mixed solution of divinylbenzene and butyl acetate to the microchannel structure at a rate of about 1.8 ml / min, and the fluid containing the ejected microdroplets is sampled to obtain 100 microdroplets. As a result of measurement, the average particle size was about 197 μm, and the particle size dispersion degree was 5.2%. In addition, the liquid feeding of the MR block was stopped while the droplet was being generated, and the MR block was stored for 1000 hours with the droplet enclosed. After storing under such unfavorable conditions, it was washed with water with a plunger pump, and liquid was sent under the same conditions as the initial stage to prepare droplets. The collected droplets were good droplets with an average particle size of about 193 μm and a particle size dispersion of 6.1%.

It is the schematic of MR block in this invention. It is a conceptual diagram of an example of the flow-path board | substrate which distributes and sends the continuous phase in this invention. It is a conceptual diagram of an example of the flow-path board | substrate which distributes and distributes the dispersed phase in this invention. It is the conceptual diagram which showed the laminated structure of packing, the microchannel substrate, and the cover body among the basic components of MR block in this invention. It is the conceptual diagram which showed the A-A 'cross section as a groove part cross section in FIG. 4 which showed three-dimensional figures, such as a flow-path board | substrate at the time of lamination | stacking. FIG. 5 is a conceptual diagram showing a B-B ′ section as a supply channel section in FIG. 4 showing a three-dimensional view of a channel substrate and the like at the time of stacking. It is the conceptual diagram which showed the C-C 'cross section as a discharge flow path cross section in FIG. 4 which showed three-dimensional figures, such as a flow-path board | substrate at the time of lamination | stacking. It is the conceptual diagram which showed the laminated structure of packing, a flow path board | substrate, and a cover body at the time of using the groove | channel board | substrate which formed the groove | channel on the upper and lower surfaces in the flow path board | substrate among the basic components of MR block in this invention. It is the conceptual diagram of the structure which formed the ring-shaped groove | channel in one surface among the basic components of MR block in this invention, and simplified positioning. It is a conceptual diagram of the flow of the fluid which flows into the one part flow path of the flow-path board | substrate of FIG. It is the schematic of the MR block using the crimping | crimping jig | tool which makes convex at least one place between the force points to crimp. 2 is an enlarged view of a microchannel substrate and microchannels used in Example 1. FIG. 2 is a photograph of droplets collected in Example 1. FIG. 6 is a schematic diagram of an MR block in Comparative Example 1. FIG. 4 is a droplet photograph collected in Comparative Example 1. 6 is a diagram illustrating a close contact state between flow path substrates in Comparative Example 1. FIG. FIG. 6 is a diagram showing a close contact state between flow path substrates in Example 2. It is an enlarged photograph of the microchannel substrate and microchannel used in Example 4. FIG. 10 is a schematic diagram of an MR block in the fifth embodiment. 6 is a schematic view of a channel of a groove substrate used in Example 6. FIG. It is the schematic of the flow path of the groove substrate used for Example 7, and the enlarged view of a micro flow path. It is the schematic of the flow path of the groove substrate used for Example 8, and the enlarged view of a micro flow path. It is an enlarged view photograph (before a through-hole process) of the flow path of the groove substrate used for Example 9.

Explanation of symbols

1: Upper crimping jig 2: Lower crimping jig 3: Outer peripheral bolt 4: Center bolt 5: Positioning pin 6: Packing 7: Continuous phase supply channel 8: Dispersed phase supply channel 9: Dispersed phase distribution channel 10: Continuous phase distribution channel 11: Dispersed phase inlet 12: Continuous phase inlet 13: Discharge channel assembly 14: Discharge port 15: Channel substrate 16: Cover body 17: Continuous phase liquid feed pump 18: Dispersed phase Liquid feed pump 19: Discharge flow channel 20: Teflon (registered trademark) tube 21: Groove substrate 22 with grooves on one side: Groove substrate 23 with different grooves on both sides 23: Cover body 24 with grooves on one side 24: Substrate Conceptual diagram of the state in which the layers are laminated 25: Conceptual diagram of the state in which the laminated flow path substrates are sandwiched between upper and lower packings 26: Notched for disassembly / cleaning, etc. 27: Cover body with ring-shaped grooves formed on one side 28: Interchange by facing the cover body Groove substrate 29 in which grooves for forming the flow path are formed: Groove substrate 30 in which a part of the flow path is formed on both surfaces: Conceptual diagram of a state in which the substrates are stacked 31: The stacked flow path substrates are sandwiched between upper and lower packings Conceptual diagram 32 of the state: Dispersed phase inlet 33 of the micro cross channel formed by combining the flow channels on both sides: Continuous phase inlet 34 of the micro cross channel formed by combining the flow channels on both sides: Combined the channels on both sides Crossing portion 35 of the micro crossing channel that can be formed: discharge port 36 of the micro crossing channel that can be formed by combining the channels on both sides: microchannel substrate 37 used in Example 1: microchannel substrate used in Example 4 38: Groove substrate used in Example 6 (groove substrate formed with a water phase and a part of the organic phase formed to have a flow path depth of 45 μm)
39: Cover body in which the groove used in Example 6 was formed (a cover body in which a partial flow path of the organic phase intersecting the water phase flow path was formed to a depth of 7 μm)
40: Conceptual diagram of a state in which a cross flow path is formed by facing the groove substrate (38) and the cover body (39) used in Example 6 41: The groove substrate and cover body used in Example 6 are Enlarged view of the flow path formed in a stacked state (intersection)
42: Groove substrate 43 used in Example 7: Enlarged flow path of substrate used in Example 7 FIG. 44: Cover body 45 with ring groove used in Example 8: Groove substrate 46 used in Example 8 : A state in which the groove substrate and the cover body used in Example 8 are laminated 47: A flow path enlarged view (part) formed in a state in which the groove substrate and the cover body used in Example 8 are laminated
48: Continuous phase storage space 49: Radial continuous phase supply flow path 50: Radial flow path tip portion communicating with 50: 7 Dispersed phase storage space 52: Radial dispersed phase supply flow channel 53: Radial upper flow path communicating with 8 Tip

Claims (19)

A microchannel structure having a fluid inlet for introducing a fluid, a microchannel for generating microparticles with the fluid, and a fluid outlet for discharging a fluid containing the generated microparticles, the microchannel structure Is composed of a fluid supply structure for supplying the fluid to the microchannel, a microchannel substrate having the microchannel, and a plate interposed between them. Droplet generation, characterized in that the material is a resin having a hardness of 70 or more in Type D according to JIS K 6253 and a molding shrinkage rate of 3% or less in accordance with JIS K 7152-4 Micro-channel assembly device. The microchannel structure includes an inlet for introducing a dispersed phase and a dispersed phase introducing microchannel that communicates with the inlet, an inlet for introducing a continuous phase and a continuous phase introducing microchannel that communicates with the inlet, and the dispersed phase introducing microchannel A droplet generating micro-channel consisting of a dispersed phase by intersecting the channel and the continuous-phase introducing micro-channel, and a fluid exhaust for discharging a fluid containing fine particles generated in communication with the droplet generating micro-channel The microchannel assembly device for generating droplets according to claim 1, further comprising an outlet. Flat jigs that are harder than the base material of the microchannel substrate are arranged above and below, and two or more upper and lower plates having a hardness of 70 or less in Type D are measured by a durometer hardness test method in accordance with JIS K 6253. 3. The droplet generating microchannel assembly device according to claim 1, further comprising a laminated body configured to sandwich the microchannel substrate therebetween. The fluid supply structure has one or more through holes as fluid inlets for introducing the fluid, and has a storage space that communicates with the fluid inlet and temporarily stores the introduced fluid. And one or more radial and linear lines for communicating fluid from the storage space to each fluid inlet of each of the one or more microchannels formed in the microchannel substrate and supplying the fluid to the microchannels 4. A supply channel formed in a curved line is provided, and at least one of the fluid supply structures is located below between the upper and lower jigs to be crimped. 2. A micro-channel assembly device for generating droplets according to 1. The microchannel substrate and a cover body that forms a channel by covering the channel formed on the microchannel substrate are brought into close contact with each other by crimping the flat jig. The micro-channel assembly device for generating droplets according to claim 3. The droplet generating micro-channel assembly device according to claim 5, wherein the cover body is harder than the micro-channel substrate. The flow path formed in the micro flow path substrate has a line or point symmetric configuration in the micro flow path substrate, and there is a space in the center of symmetry, so that the micro flow path substrate is crimped. The micro-channel assembly device for droplet generation according to claim 5 or 6, further comprising a jig. 8. The micro-channel assembly device for droplet generation according to claim 5, further comprising a positioning mechanism for three-dimensionally stacking the micro-channel substrates or installing a cover body. The droplet generation according to any one of claims 5 to 8, wherein a part of the microchannel substrate has a recess for inserting a jig for separating the microchannel substrates from the stacked state. Micro-channel assembly device. Holes communicating with the plate interposed between the fluid supply structure and the microchannel substrate are arranged on the plane of the crimping jig, and the fluid supply structure and the microchannel substrate 10. The micro-channel assembly device for generating droplets according to claim 1, wherein the holes are provided in a plate interposed therebetween so that the holes are aligned at a time. 11. The microchannel for generating droplets according to claim 1, wherein a plate interposed between the fluid supply structure and the microchannel substrate is smaller than the microchannel substrate. Aggregate device. The microchannel assembly device for generating droplets according to any one of claims 1 to 11, wherein the surface of the channel formed on the microchannel substrate is subjected to a hydrophilic treatment. A cover that covers the flow path formed on the micro flow path substrate on the back surface of the flow path forming surface of the micro flow path substrate different from the micro flow path substrate when the micro flow path substrate is stacked. The microchannel assembly device for generating droplets according to any one of claims 5 to 12, wherein the microchannel assembly device is formed as a body. The depth of the flow path from the vertical hole flow path (through hole) communicating with the fluid supply structure to the introduction portion of the introduction micro flow path is deeper than the depth of the micro flow path for droplet generation. The microchannel assembly device for droplet generation according to any one of 1 to 7. 15. The droplet generating micro-channel assembly device according to claim 1, wherein a part of the channel is deep in the discharge channel formed on the micro-channel substrate. . When two microchannel substrates having different channel structures face each other on the surfaces where the channels are formed, the liquid at the portion where the channels on the microchannel substrates with different channels intersect The microchannel assembly device for generating droplets according to any one of claims 1 to 15, which has a structure for forming droplets. 17. The micro-channel assembly device for generating droplets according to claim 16, wherein the channel structure on one micro-channel substrate is annular and has no start point and no end point. 18. The micro-channel assembly device for generating droplets according to claim 17, wherein the grooves of one of the channel substrates are concentric circles. The flow path for introducing the dispersed phase or continuous phase into the micro flow path substrate is positioned below the flow path substrate, and the liquid feeding direction of the fluid discharged through each substrate is upward. 18. A micro-channel assembly device for generating droplets according to any one of 18 above.

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