JP2008222643A - Liposome for cosmetic use - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a liposome for cosmetics better than conventional ones. <P>SOLUTION: The liposome for cosmetic use is characterized in that a cyclic phosphatidic acid represented by formula [I] [wherein, R is a 10-22C acyl; and M is H, an alkali metal atom, alkaline earth metal atom or a (substituted) ammonium group] is compounded as a constituent of the liposome. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a cosmetic liposome in which cyclic phosphatidic acid having a specific structure is blended as a component of the liposome, and a cosmetic containing the cosmetic liposome.

If the skin ages due to ultraviolet rays, stress, or aging, the skin will lose its firmness, dry and lose its smoothness, and the skin may become rough and wrinkled. , Spots and the like will occur. In addition, the opening of pores becomes conspicuous with aging and the fineness of the skin is lost.
Hyaluronic acid, which has the function of maintaining moisture and flexibility between cells, contributes to the firmness and freshness of the skin. Therefore, the hyaluronic acid in the epidermis and dermis is responsible for the above-mentioned skin aging phenomenon. The decrease in acid is said to be affected. For this reason, attempts have been made to replenish hyaluronic acid from outside the body and supplement substances to improve hyaluronic acid production as measures against skin aging.
Among them, Patent Document 1 describes that cyclic phosphatidic acid has an effect of promoting hyaluronic acid production in aged skin and improving skin firmness. However, since cyclic phosphatidic acid has low solubility in water, an aurora is produced even in a small amount in an aqueous system, and it has been difficult to incorporate a large amount in a system with a large amount of water.

Patent Document 2 and Patent Document 3 which is a divisional application thereof disclose a technique for externally applying 1-O-acyl lysophosphatidic acid or cyclic phosphatidic acid ester to the skin in anticipation of an improvement effect such as anti-wrinkle on the skin. Has been. However, in these documents, since 1-O-acyl lysophosphatidic acid and cyclic phosphatidic acid ester are not made into liposomes, a smooth feel is not obtained.
JP 2002-363081 A US Pat. No. 5,238,965 US Pat. No. 5,512,223

  Cyclic phosphatidic acid has low solubility in water, and it has been difficult to add a large amount to cosmetics composed of a water-rich system. Moreover, the cosmetics using the conventional cyclic phosphatidic acid do not make use of the smooth touch that is a characteristic of the cyclic phosphatidic acid, and there is room for improvement in terms of the touch when used.

  As a result of earnest research to obtain a cosmetic material containing cyclic phosphatidic acid that is excellent and stable in use as a cosmetic, the present inventors have formulated a liposome by blending a cyclic phosphatidic acid having a specific structure as a component of the liposome. When produced and blended with cosmetics, it was found that a cosmetic having excellent usability and stability was obtained, and the present invention was completed.

That is, the gist of the present invention is as follows.
(1) A liposome for cosmetics, wherein a cyclic phosphatidic acid represented by the following formula [I] is blended as a constituent of the liposome:

(Where
R represents an acyl group having 10 to 22 carbon atoms;
M represents a hydrogen atom, an alkali metal atom, an alkaline earth metal atom, or a substituted or unsubstituted ammonium group. ).
(2) The liposome for cosmetics according to (1) above, further comprising phospholipids as a component of the liposome.
(3) The liposome for cosmetics according to (1) or (2) above, wherein sterols are further blended as a component of the liposome.
(4) A cosmetic comprising the cosmetic liposome according to any one of (1) to (3) above.

  The liposome for cosmetics according to the present invention is characterized by the fact that cyclic phosphatidic acid having a specific structure is blended as a constituent component of the liposome, so that when used as a cosmetic, it improves skin roughness and has a plump and moisturized smoothness. In addition to being able to lead to smooth skin, it is possible to obtain a cosmetic having a smooth use feeling that is more stable with time (no aurora) than conventional products and has no stickiness. Moreover, the cosmetic containing the liposome for cosmetics of the present invention has an effect of improving skin firmness and an effect of tightening pores, has a high moisturizing property, and has a good feeling of use.

  The liposome for cosmetics of the present invention is characterized in that cyclic phosphatidic acid represented by the following formula [I] is blended as a component of the liposome.

(Where
R represents an acyl group having 10 to 22 carbon atoms;
M represents a hydrogen atom, an alkali metal atom, an alkaline earth metal atom, or a substituted or unsubstituted ammonium group. )

  In the cosmetic liposome of the present invention, the cyclic phosphatidic acid of the formula [I] is preferably blended in an amount of 0.000001 to 5% of the total raw material at the time of producing the liposome, and is blended in a range of 0.00001 to 3%. More preferably. When the blending ratio of the cyclic phosphatidic acid of the formula [I] exceeds 5% of the total, the cyclic phosphatidic acid of the formula [I] has a property as a linear surfactant, so that the structure of the liposome is broken. there is a possibility. Further, if the blending ratio is less than 0.000001% of the total, the smooth feel that is a characteristic of the cyclic phosphatidic acid of the formula [I] may be weakened due to the influence of other components.

Examples of the acyl group in the formula [I] include a saturated acyl group having 10 to 22 carbon atoms and an acyl group having 10 to 22 carbon atoms and 1 to 6 unsaturated bonds. A saturated acyl group is preferable, and a saturated acyl group having 14 to 18 carbon atoms is more preferable. Saturated acyl groups are preferred because deterioration such as oxidation is unlikely to occur and changes over time such as color and odor are unlikely to occur.
Examples of the saturated acyl group having 10 to 22 carbon atoms include capric acid (C10: 0), lauric acid (C12: 0), myristic acid (C14: 0), pentadecanoic acid (C15: 0), and palmitic acid (C16: 0). ), Margaric acid (C17: 0), stearic acid (C18: 0), nonadecanoic acid (C19: 0), arachidic acid (C20: 0), heneicosanoic acid (C21: 0), behenic acid (C22: 0), etc. The residue derived from is mentioned. Among these, myristic acid (C14: 0), pentadecanoic acid (C15: 0), palmitic acid (C16: 0), and stearic acid (C18: 0) are considered in terms of ease of handling and strength of activity. A saturated acyl group having 14 to 18 carbon atoms such as a residue derived therefrom is preferred.
Examples of the acyl group having 10 to 22 carbon atoms and 1 to 6 unsaturated bonds include myristoleic acid (C14: 1), palmitoleic acid (C16: 1), oleic acid (C18: 1), and elaidic acid (C18 1), vaccenic acid (C18: 1), linoleic acid (C18: 2), linolenic acid (C18: 3), arachidonic acid (C20: 4), eicosapentaenoic acid (C20: 5), erucic acid (C22: 1), residues derived from docosahexaenoic acid (C22: 6) and the like. Among them, myristoleic acid (C14: 1), palmitoleic acid (C16: 1), oleic acid (C18: 1), elaidic acid (C18: 1), linoleic acid (C18: 2), linolenic acid (C18: 3) ) Is preferred.

Examples of the alkali metal atom in the formula [I] include sodium and potassium.
Examples of the alkaline earth metal atom in the formula [I] include magnesium and calcium.
The ammonium group in the formula [I] is an atomic group represented by NH ₄ and may have a substituent or may be unsubstituted.
Examples of the substituent of the ammonium group include triethanolamine salt.
M in the formula [I] is most preferably sodium or potassium from the viewpoint of easy availability as a phosphoric ester derivative of the cyclic phosphatidic acid represented by the formula [I].

  The cyclic phosphatidic acid of the formula [I] used in the present invention can be synthesized by a known production method. For example, it can be obtained by total synthesis in which —O-acylglycerol is reacted with a phosphorylating agent and hydrolyzed (see JP-A-6-2281691). Moreover, what was obtained by the enzyme reaction as described in patent 3195833 can also be used.

  Specifically, for example, by reacting lysophosphatidylcholine derived from hydrogenated soybean phospholipid and enzyme phospholipase D in an acetic acid buffer (pH of about 5 to 6) for about 1 to 20 hours, a formula derived from hydrogenated soybean [ I] cyclic phosphatidic acid can be obtained. In addition, the produced cyclic phosphatidic acid of the formula [I] can be purified by extraction, column chromatography, thin layer chromatography (TLC) and the like according to a conventional method.

  When the liposome for cosmetics in the present invention contains phospholipid, naturally-derived phospholipids such as soybean phospholipid, egg yolk phospholipid, hydrogenated soybean phospholipid, hydrogenated egg yolk phospholipid, phosphatidylcholine, phosphatidylethanolamine, Phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, sphingomyelin and the like can be used. In the case of naturally derived phospholipids, hydrogenated soybean phospholipids having a phosphatidylcholine content of 60% by weight or more are preferable, and hydrogenated soybean phospholipids having a phosphatidylcholine content of 80% by weight or more are more preferable.

  The hydrogenated soybean phospholipid can be obtained, for example, by extracting and purifying soybean phospholipid by two-phase partitioning between water and hexane, alcohol, and the like, and then hydrogenating it. Further, the hydrogenated soybean phospholipid may be obtained by crystallization purification using a solvent such as acetone or alcohol, or may be obtained by separation and purification by liquid chromatography or the like.

  The liposome for cosmetics of the present invention preferably contains a phospholipid as a component of the liposome. As a compounding quantity, it is preferable to mix | blend 0.001-20 weight% of the whole raw material at the time of liposome manufacture, It is more preferable to mix | blend 0.005-10 weight%, It is compounding 0.01-5 weight%. Further preferred. By adding 0.001% by weight or more of phospholipid, it is possible to obtain a touch and moisturizing effect peculiar to liposomes, and when it is contained by 20% by weight, a sufficient moisturizing effect can be retained. All blends are costly.

  Since the liposome for cosmetics of this invention can suppress the fluidity | liquidity of a film | membrane and can improve stability with time, it is preferable to mix | blend sterols as a structural component of a liposome. Examples of sterols include cholesterol, phytosterol, dihydrocholesterol, cholesteryl stearate, cholesteryl oleate, cholesteryl nonanoate, cholesteryl hydroxystearate, dihydrocholesteryl oleate, among others, cholesterol, phytosterol, cholesteryl oleate Cholesteryl stearate is preferred. The cosmetic liposome of the present invention more preferably contains sterols in an amount of 1 to 50% by weight based on the phospholipid.

  When the cosmetic liposome of the present invention is composed of cyclic phosphatidic acid of formula [I], phospholipid, and sterols, the ratio of phospholipid to sterols is 5/5 to 9.9 / by weight. 0.1 is preferable, 6/4 to 9/1 is more preferable, and 7/3 to 8/2 is still more preferable.

In the liposome for cosmetics of the present invention, in addition to the components specially mentioned so far, components that are approved for incorporation into cosmetics, for example, aqueous components (as long as the effects of the present invention are not hindered) For example, water, etc.), alcohol (eg, ethanol, glycerin, etc.), oil agent, surfactant, water-soluble polymer, natural extracts derived from animals and plants, various nutrients, moisturizer, cell activator, UV absorber, antioxidant You may mix | blend an agent, antiseptic | preservative, a fragrance | flavor, an inorganic salt, various chemicals according to the objective, etc. The production method is not particularly limited as long as a method usually used for producing cosmetics may be used.
The specially mentioned constituents and these additional components may be present in the inner aqueous phase or the outer aqueous phase of the liposome, on both sides thereof, or may be part of the liposome membrane.

  The cosmetic liposome of the present invention is prepared by, for example, mixing a cyclic phosphatidic acid of the formula [I] with a lipid (for example, phospholipid) and other compounding ingredients by a known technique for preparing a liposome, and subjecting it to ultrasonic treatment. Method, French press method, reverse phase evaporation method, surfactant method, extrusion method, calcium-EDTA chelate method, freeze-thaw method, vacuum emulsifier, high-pressure homomixer, microfluidizer, manton gorin, etc. It can manufacture by processing with an emulsifier. In addition, by performing sizing operations such as filtering and gel filtration on these liposomes, it is possible to selectively obtain liposomes having a uniform particle diameter, thereby further improving the stability of the liposomes.

  Since the liposome particle size also affects the stability and feel, the cosmetic liposome of the present invention desirably has the same particle size. As a method for displaying the particle diameter of liposomes, a weight average particle diameter and a number average particle diameter are generally used. From the viewpoint of the retention capacity of liposomes, it is desirable to represent the weight average particle diameter. The weight average particle diameter of the liposome for cosmetics of the present invention is generally 20 nm to 800 nm, preferably 50 to 500 nm, more preferably 100 nm to 400 nm. In order to obtain the desired particle size, an extrusion filtration method or the like that filters liposomes having a large particle size through a filter having a predetermined pore size may be used.

  The liposome for cosmetics of the present invention can be widely used as a raw material for cosmetics from low viscosity such as lotion to high viscosity such as cream. Since the liposome for cosmetics of the present invention has good stability, there is no problem even if it is blended in a low viscosity lotion.

The liposome for cosmetics of the present invention cannot be defined unconditionally because it varies depending on the form of the cosmetic, but it is preferable to blend 0.001 to 20% by weight of the entire cosmetic, 0.005 to 10% by weight More preferably, it is more preferably 0.01 to 5% by weight. When the amount is less than 0.001% by weight, the effect of improving the smoothness of the skin and the moisturizing property of the liposome for cosmetics of the present invention is hardly reflected in the cosmetics. Further, if 20% by weight is incorporated, these effects can be sufficiently exerted, and therefore the blending exceeding 20% by weight is disadvantageous from the viewpoint of cost effectiveness.
The liposome for cosmetics of the present invention can be contained in various types of cosmetics, and the form of the cosmetics is not particularly limited.

  The cosmetic containing the cosmetic liposome of the present invention has a smooth use feeling, can improve that skin, and can lead to a smooth, moist and smooth skin.

  EXAMPLES Hereinafter, although an Example is described and this invention is demonstrated concretely, this invention is not limited to these. In addition, unless otherwise indicated, the compounding quantity of each component in each Example and a comparative example is weight%.

Synthesis example
Synthesis of lysophosphatidylcholine Phospholipids derived from hydrogenated soybeans (phosphatidylcholine content 96%, fatty acid composition: palmitic acid 11.5%, margaric acid 0.2%, stearic acid 87.8%, linoleic acid 0.3%, other minor components 0.2%; also used in Comparative Example 4) 300 g of chloroform was added to 20 g and sufficiently dissolved. 120 g of 1M Tris-HCl buffer at pH 8.5, 10 mL of 2M calcium chloride and 2 g of phospholipase A2 (lecitase, manufactured by Novozymes) were added and heated at 50 ° C. After 24 hours, 600 g of methanol was added and mixed vigorously. After mixing, the mixture was allowed to stand to recover the lower layer, and the solvent was distilled off to obtain 15 g of a solid. 15 g of this solid was added to 200 g of ethyl acetate and stirred at 45 ° C. for 1 hour, followed by filtration to obtain 14 g of a solid. Further, this operation was repeated to obtain 11 g of solid (hydrogenated soybean-derived lysophosphatidylcholine).
Fatty acid composition of the obtained hydrogenated soybean-derived lysophosphatidylcholine: palmitic acid 19.3%, stearic acid 80%, margaric acid 0.2%, linoleic acid 0.3%, others 0.2%

Synthesis of cyclic sodium phosphatidic acid 250 g of water was sufficiently dissolved in 10 g of hydrogenated soybean-derived lysophosphatidylcholine (LPC) obtained above. 100 g of 1M sodium acetate buffer solution at pH 5.5, 10 mL of 2M sodium chloride, and 100 mg of phospholipase D (PL-D, Meisei Sangyo) were added and heated at 55 ° C. After 18 hours, the solution was neutralized with 10M aqueous sodium hydroxide solution to pH 6.5, and then 650 g of chloroform and 700 g of methanol were added and mixed vigorously. Further, 650 g of chloroform was added and mixed, and then allowed to stand. The lower layer was recovered and the solvent was distilled off to obtain 8 g of cyclic sodium phosphatidate. This cyclic sodium phosphatidate was used in the following examples and comparative examples.
Fatty acid composition of the obtained cyclic sodium phosphatidate: 19.3% palmitic acid, 80% stearic acid, 0.2% margaric acid, 0.3% linoleic acid, other 0.2%

Example 1
After mixing the components 1-7 of the quantity shown in Table 1 and heat-melting at 60 degreeC, the component 8 (purified water) was added and it stirred at 60 degreeC for 30 minutes. The obtained dispersion was subjected to extrusion using a polycarbonate filter having a pore size of 0.4 μm to obtain the liposome solution of Example 1.

Examples 2-4
The liposome solutions of Examples 2 to 4 shown in Table 1 were produced in the same manner as in Example 1. However, regarding the extrusion, the pore diameter was 0.3 μm in Examples 2 and 3, and 0.2 μm in Example 4. Polycarbonate filters were used.

Examples 5 and 6
After mixing the components 1-7 of the quantity shown in Table 1, and heat-melting at 60 degreeC, the component 8 (purified water) is added, homogenization is performed for 30 minutes at 60 degreeC, and the liposome liquid of Examples 5 and 6 Got.

Comparative Example 1
After mixing the components 1 to 10 in the amounts shown in Table 2 and heating and melting at 60 ° C., component 11 (purified water) is added, homogenized at 60 ° C. for 10 minutes, and the cyclic phosphatidic acid aqueous solution of Comparative Example 1 Got.

Comparative Examples 2 and 3
After mixing the components 1 to 10 in the amounts shown in Table 2 and heating and melting at 60 ° C., component 11 (purified water) is added, emulsified with a high-pressure emulsifier, and the cyclic phosphatidic acid emulsions of Comparative Examples 2 and 3 Got.
Comparative Example 4
After mixing the components 1-10 of the quantity shown in Table 2 and heat-melting at 60 degreeC, the component 11 (purified water) was added and it stirred at 60 degreeC for 30 minutes. The obtained dispersion was subjected to extrusion using a polycarbonate filter having a pore size of 0.4 μm to obtain a liposome solution of Comparative Example 4.

  The following evaluations were performed on the sample solutions obtained in the examples and comparative examples, and the results are also shown in Tables 1 and 2. In addition, the components 1-3 of Table 1 are substances which become a component which comprises a liposome. In addition, components 5 and 6 in Table 2 are main components of the liposome, and form a lipid bilayer. In Table 2, components 1 to 6 are components that can be constituents of liposomes, but if components 5 and 6 are not present, components 1 to 4 do not form a lipid bilayer. Not taken.

〔Stability〕
The sample solutions of Examples 1 to 6 or Comparative Examples 1 to 4 were placed in a transparent glass vial, sealed, and left in a constant temperature bath at 40 ° C. with light shielding. The presence or absence of aurora in the solution during storage at 40 ° C. was examined. Each evaluation was performed according to the following criteria.
<Criteria A>
○: Aurora is not recognized for more than 1 month.
Δ: No change was observed until 2 weeks, but aurora was observed thereafter.
X: Aurora was recognized in 2 weeks.

[Evaluation of feeling of use]
About the feeling of use (no stickiness, moist feeling) when the sample liquids of Examples 1 to 6 or Comparative Examples 1 to 4 were applied to the skin, the average score was evaluated by 10 women. Was determined according to criteria B. ◎ or ○ was determined to be effective.
<Evaluation>
5 points: very good 4 points: good 3 points: normal 2 points: defective 1 point: very bad <reference B>
◎: Average point 4.5 points or more ○: Average point 3.5 points or more and less than 4.5 points △: Average point 2.5 points or more and less than 3.5 points ×: Average point less than 2.5 points

[Effect]
Example 1 and Comparative Example 1, Example 2 and Comparative Example 1 on the left and right cheeks twice a day (morning and evening) for five women (25 to 55 years old) who care about rough skin and dryness Example 3 and Comparative Example 2, Example 4 and Comparative Example 2, Example 5 and Comparative Example 3, Example 6 and Comparative Example 4 were each applied in one cheek, and the improvement effect on the left and right skin was evaluated. . The improvement effect was evaluated according to the following evaluation criteria for “smoothness of skin” and “skinness” of the skin state one month after application, and the average score was determined according to criteria C. ◎ or ○ was determined to be effective.
<Evaluation>
3 points: 2 points where the effect was felt 2 points: 1 point where the effect was felt: 0 points which cannot be said to be 0: No effect was felt <Criteria C>
◎: Average point 2.5 points or more ○: Average point 1.5 points or more and less than 2.5 points △: Average point 0.5 points or more and less than 1.5 points ×: Average point less than 0.5 points

The liposome solutions of Examples 1 to 6 satisfied all the performances of stability, feeling of use and effect.
When the aqueous solution of Comparative Example 1 was allowed to cool after preparation, aurora was produced. In addition, the emulsions of Comparative Examples 2 and 3 and the liposome solution of Comparative Example 4 were inferior in usability and / or effect compared to the liposome solution of Examples.

[Comparison of skin firmness and pore tightening effect]
For five women who care about the opening of pores, the sample solution of Example or Comparative Example was applied to the entire face twice a day (morning and evening) for 2 months.
The skin condition after 2 months of 5 subjects was evaluated by observing a photograph of the skin and a microscope image with the naked eye by 3 evaluators. As for skin firmness, a photograph of the skin was used to evaluate the improvement effect when the position of the cheek cone increased, and the average score was obtained and shown in Table 3. Regarding tightening of pores, using a microscope image, paying attention to the shape of the pores, unevenness, and smoothness of the surface, compared with before continuous use, the improvement effect was evaluated according to the following evaluation criteria, and the average score was calculated. The results are shown in Table 3.
<Evaluation>
5 points: 3 points where the effect was felt very much: 1 point where the effect was felt: 0 points that cannot be said to be 0: No effect was felt

  It was found that the liposome solutions of the examples had an excellent improvement effect in terms of skin firmness and pore tightening.

Examples 7-10, Comparative Examples 5-8
Using the sample liquids of the above Examples or Comparative Examples, the lotions shown in Table 4 (Examples 7 and 8, Comparative Examples 5 and 6) and the creams shown in Table 5 (Examples 9 and 10, Comparative Examples 7 and 8) ) Was produced.
[Manufacturing method of lotion]
1) Components 1, 2 and 6 were dissolved in component 8 (purified water) shown in Table 4 at room temperature.
2) Components 3 to 5 and 7 were mixed and solubilized in the mixture of 1).

[Production method of cream]
1) Components 1 to 8 shown in Table 5 were heated to 80 ° C. and kneaded well.
2) Components 9 to 19 were also heated to 80 ° C. and kneaded well.
3) 1) and 2) were mixed and dispersed, and cooled to room temperature to obtain a cream.

  About the said skin lotion and cream, the feeling of use and the improvement effect of the skin state were evaluated, respectively. The results are shown in Tables 6 and 7, respectively.

[Evaluation of feeling of use]
The feeling of use (no stickiness / smoothness of skin) when the cosmetics of Examples 7 to 10 and Comparative Examples 5 to 8 were applied to the skin was evaluated by the 10 panelists in the following 5 levels. Average points were determined according to criteria D. ◎ or ○ was determined to be effective.
<Evaluation>
5 points: very good 4 points: good 3 points: normal 2 points: bad 1 point: very bad

[Improvement effect on skin condition]
Example 7 and Comparative Example 4, Example 8 and Comparative Example 5 on the left and right cheeks twice a day (morning and evening) for five women (25 to 55 years old) who care about rough skin and dryness Each combination of Example 9 and Comparative Example 6 was applied on one cheek, and the left and right skin improvement effects were evaluated. The improvement effect in terms of “smoothness of skin” and “skin elasticity” one month after application was evaluated according to the following evaluation criteria, and the average score was determined according to criteria D. ◎ or ○ was determined to be effective.
<Evaluation>
3 points: 2 points where the effect was felt 2 points: 1 point where the effect was felt: 0 points that cannot be said to be 0: No effect was felt <Criteria D>
◎: Average point 2.5 points or more ○: Average point 1.5 points or more and less than 2.5 points △: Average point 0.5 points or more and less than 1.5 points ×: Average point less than 0.5 points

The skin lotion produced using the sample liquid of the example satisfied all the feelings of use and effects.
On the other hand, the lotion produced using the sample liquid of the comparative example was inferior in the feeling of use and / or the effect on the skin.

The cream produced using the sample solution of the example satisfied all the feelings of use and effects.
The cream produced using the sample solution of the comparative example felt strongly sticky and had a poor effect on the skin.

Claims (4)

A liposome for cosmetics characterized in that a cyclic phosphatidic acid represented by the following formula [I] is blended as a component of the liposome:

(Where
R represents an acyl group having 10 to 22 carbon atoms;
M represents a hydrogen atom, an alkali metal atom, an alkaline earth metal atom, or a substituted or unsubstituted ammonium group. ).   Furthermore, the lipolipid is mix | blended as a structural component of a liposome, The liposome for cosmetics of Claim 1 characterized by the above-mentioned.   Furthermore, sterols are mix | blended as a structural component of a liposome, The liposome for cosmetics of Claim 1 or 2 characterized by the above-mentioned.   Cosmetics containing the liposome for cosmetics of any one of Claims 1-3.