JP2008161070A - Soymilk fermented product, and homogeneous composition containing the same - Google Patents

Soymilk fermented product, and homogeneous composition containing the same Download PDF

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JP2008161070A
JP2008161070A JP2006351102A JP2006351102A JP2008161070A JP 2008161070 A JP2008161070 A JP 2008161070A JP 2006351102 A JP2006351102 A JP 2006351102A JP 2006351102 A JP2006351102 A JP 2006351102A JP 2008161070 A JP2008161070 A JP 2008161070A
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soymilk
fermented product
particle size
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homogeneous composition
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Toshinori Igarashi
俊教 五十嵐
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Kikkoman Corp
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Kikkoman Corp
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a soymilk fermented product excellent in homogeneity stability even if it is preserved for a long period, without adding special water-soluble hemicellulose and hydrolyzing soymilk with protease, and to provide a homogeneous composition containing the same. <P>SOLUTION: This soymilk fermented product is obtained by inoculating lactic acid bacteria to soymilk followed by culturing the product, and has ≤10% of particles with a particle diameter of ≥80 μm in the particle size distribution. The soymilk fermented product-containing homogeneous composition is obtained by mixing aqueous seasoning liquid which contains common salt and vinegar with the soymilk fermented product. The soymilk fermented product-containing homogeneous composition which is excellent in homogeneity stability even under a low pH condition and has excellent palate feeling can be obtained by mixing the soymilk fermented product preferably at 5-75% (w/w), based on the whole of the soymilk fermented product-containing homogeneous composition. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

本発明は、低pHにおいても均質安定性に優れた豆乳発酵物及びそれを含有する均質組成物に関する。   The present invention relates to a fermented soymilk excellent in homogeneous stability even at low pH and a homogeneous composition containing the same.

豆乳は、低コレステロール、低カロリーで高蛋白の健康食品として注目されており、豆乳特有のにおいや味の問題を回避又は軽減するために、豆乳を乳酸発酵させたり、酸性果汁や有機酸を加えたりする等の試みが従来なされてきた。
そして、豆乳蛋白質の酸性下での安定性が充分でないことにより発酵豆乳や酸性条件下の豆乳が有していた、凝集やざらつき、分離を生じやすいという欠点を解消するため、特殊な水溶性ヘミセルロースを添加する方法(例えば、特許文献1参照)、あるいは豆乳を予めプロテアーゼで酵素分解し、次いで乳酸菌による乳酸発酵を行う方法(例えば、特許文献2参照)が開示されてきた。しかし、前記のような付加的な処理工程を行うことなく得られる、低pH条件下で長期保存しても均質安定性に優れた豆乳発酵物及びそれを含有する均質組成物は知られていない。
特開2001−29038号公報 特開昭61−63260号公報
Soy milk is attracting attention as a low-cholesterol, low-calorie, high-protein health food, and soy milk can be lactic acid fermented, acid juice or organic acids added to avoid or reduce the odor and taste problems unique to soy milk. Attempts have been made in the past.
In order to eliminate the disadvantages of fermented soy milk and soy milk under acidic conditions due to insufficient acid stability of soy milk protein, it is easy to cause aggregation, roughness, and separation. Has been disclosed (for example, see Patent Document 1), or a method in which soymilk is previously enzymatically decomposed with a protease and then lactic acid fermentation with lactic acid bacteria is performed (for example, see Patent Document 2). However, a soymilk fermented product and a homogeneous composition containing the same, which are obtained without performing the above-described additional processing steps and are excellent in homogeneous stability even when stored for a long time under low pH conditions, are not known. .
JP 2001-29038 A JP 61-63260 A

本発明は、特殊な水溶性ヘミセルロースの添加や、プロテアーゼによる豆乳の加水分解を行うことなく、低pH条件下で長期保存しても均質安定性に優れた豆乳発酵物及びそれを含有する均質組成物を得ることを課題とする。   The present invention is a soymilk fermented product excellent in homogeneous stability even when stored for a long time under low pH conditions without adding special water-soluble hemicellulose or hydrolyzing soymilk with protease, and a homogeneous composition containing the same The task is to get things.

本発明者らは、前記課題解決のために鋭意研究を重ねた結果、豆乳に乳酸菌を接種培養して得られる豆乳発酵物であって、その粒度分布において80μm以上の粒子径を有する粒子が10%以下である豆乳発酵物及びそれを含有する均質組成物が、低pH条件下で長期保存しても凝集やざらつき、分離を生じない優れた均質安定性を有することを知り、この知見に基づき本発明を完成した。   As a result of intensive studies to solve the above problems, the present inventors have obtained a soymilk fermented product obtained by inoculating and culturing soymilk with lactic acid bacteria, and 10 particles having a particle size of 80 μm or more in the particle size distribution are obtained. % Soymilk fermented product and a homogeneous composition containing the same have excellent homogeneous stability that does not cause aggregation, roughness, and separation even when stored for a long time under low pH conditions. The present invention has been completed.

すなわち本発明は、以下に関する。
(1)豆乳に乳酸菌を接種培養して得られる豆乳発酵物であって、その粒度分布において80μm以上の粒子径を有する粒子が10%以下であることを特徴とする豆乳発酵物。
(2)乳酸菌がLeuconostoc属に属する微生物であるである前記(1)に記載の豆乳発酵物。
(3)豆乳に乳酸菌を接種培養して得られる豆乳発酵物であって、その粒度分布において80μm以上の粒子径を有する粒子が10%以下である豆乳発酵物と水性調味液とを含有し、pHが3.0〜4.7以下である豆乳発酵物含有均質組成物。
(4)豆乳に乳酸菌を接種培養して得られる豆乳発酵物であって、その粒度分布において80μm以上の粒子径を有する粒子が10%以下である豆乳発酵物を、豆乳発酵物含有均質組成物全量に対して5〜75%(w/w)含有することを特徴とする前記(3)に記載の豆乳発酵物含有均質組成物。
That is, the present invention relates to the following.
(1) A soymilk fermented product obtained by inoculating and cultivating lactic acid bacteria in soymilk, wherein the particles having a particle size of 80 μm or more in the particle size distribution is 10% or less.
(2) The fermented soymilk according to (1), wherein the lactic acid bacteria are microorganisms belonging to the genus Leuconostoc.
(3) a soymilk fermented product obtained by inoculating and cultivating lactic acid bacteria in soymilk, comprising a soymilk fermented product having an particle size distribution of 80 μm or more and a particle size distribution of 10% or less and an aqueous seasoning liquid, A soymilk fermented product-containing homogeneous composition having a pH of 3.0 to 4.7 or less.
(4) A soymilk fermented product obtained by inoculating and cultivating lactic acid bacteria in soymilk, wherein the soymilk fermented product having a particle size distribution of 80 μm or more and 10% or less of the particle diameter is a soymilk fermented product-containing homogeneous composition The soymilk fermented product-containing homogeneous composition as described in (3) above, comprising 5 to 75% (w / w) based on the total amount.

本発明によれば、特殊な水溶性ヘミセルロースの添加や、プロテアーゼによる豆乳の加水分解を行うことなく、低pH条件下で長期保存しても均質安定性に優れた豆乳発酵物及びそれを含有する均質組成物を容易に得ることができる。   According to the present invention, a soymilk fermented product having excellent homogeneity and stability even if stored for a long time under low pH conditions without adding special water-soluble hemicellulose or hydrolyzing soymilk with a protease, and the same A homogeneous composition can be easily obtained.

以下、本発明の実施の形態について詳細に説明する。   Hereinafter, embodiments of the present invention will be described in detail.

(豆乳)
本発明の豆乳発酵物に使用する豆乳としては、任意の豆乳が使用でき、例えば、3%(w/w)以上の大豆固形分を含有する豆乳を使用することができる。大豆固形分とは、水分を除いた大豆の成分の量で、炭水化物、脂質、たんぱく質を主成分とするものであり、JAS(日本農林規格)によれば豆乳飲料(その他)の大豆固形分は4%以上と定められている。本発明では、市販の各種豆乳やその原料として一般的な大豆固形分濃度、例えば4〜16%(w/w)の豆乳を好適に使用することができる。また、必要に応じ、より大豆固形分含量の高い豆乳を使用してもよい。
豆乳には、例えば単糖類、ニ糖類、オリゴ糖等の1種以上の糖を添加することができる。これらの糖は、発酵工程において乳酸菌に資化させて増殖を促すために添加する。糖の例としてはシュークロース、マルトース、スタキオース又はラフィノース等があり、乳酸菌に応じて適宜選択すればよい。糖の添加量は特に限定されないが、例えばLeuconostoc pseudomesenteroides(NISL7223)を用いる場合、豆乳に対し3%(w/w)以上、例えば5〜15%(w/w)程度のシュークロースを添加することによって、本発明の豆乳発酵物を容易に得ることができる。好適な添加量範囲の下限を下回る添加量では乳酸菌の生育が効率よく行われず、逆に好適な添加量範囲の上限を上回る添加量では、添加した糖を資化しきれず豆乳発酵物中に残留する傾向を有するので、使用する乳酸菌によって好適な添加量範囲を決定することが好ましい。
(Soy milk)
As the soy milk used in the fermented soy milk of the present invention, any soy milk can be used. For example, soy milk containing 3% (w / w) or more of soy solids can be used. Soybean solid content is the amount of soy components excluding moisture, which is mainly composed of carbohydrates, lipids, and proteins. According to JAS (Japan Agricultural Standards), soymilk beverage (others) It is set at 4% or more. In the present invention, various types of commercially available soy milk and soy milk having a general soybean solid concentration, for example, 4 to 16% (w / w), can be suitably used. Moreover, you may use soy milk with higher soybean solid content as needed.
One or more sugars such as monosaccharides, disaccharides and oligosaccharides can be added to soy milk. These sugars are added in order to promote the growth by assimilating to lactic acid bacteria in the fermentation process. Examples of sugar include sucrose, maltose, stachyose, raffinose, etc., and may be appropriately selected according to lactic acid bacteria. The amount of sugar added is not particularly limited. For example, when Leuconostoc pseudomesenteroides (NISL7223) is used, 3% (w / w) or more, for example, about 5 to 15% (w / w) of sucrose should be added to soy milk. Thus, the soymilk fermented product of the present invention can be easily obtained. The addition amount below the lower limit of the preferred range of addition does not allow lactic acid bacteria to grow efficiently. Conversely, if the addition amount exceeds the upper limit of the preferred range of addition, the added sugar cannot be assimilated and remains in the fermented soymilk. Therefore, it is preferable to determine a suitable addition amount range depending on the lactic acid bacteria to be used.

(乳酸菌)
粒度分布において80μm以上の粒子径を有する粒子が10%以下である本発明の豆乳発酵物は、豆乳に乳酸菌を添加して乳酸発酵を行わせることにより得ることができる。使用する乳酸菌としては、豆乳に接種培養した場合に、その粒度分布において80μm以上の粒子径を有する粒子が10%以下である豆乳発酵物を生産可能な乳酸菌であれば任意の乳酸菌が利用可能である。例えば、Leuconostoc属に属する微生物が挙げられる。具体的な種としては、例えば、Leuconostoc mesenteroides、Leuconostoc pseudomesenteroides、Leuconostoc citreum、Leuconostoc dextranicum等が挙げられ、より具体的には、例えば、Leuconostoc mesenteroides(NISL 7219、NISL 7200、NRIC 1539)、Leuconostoc pseudomesenteroides(NISL7223)、Leuconostoc citreum(NRIC 1579)、Leuconostoc dextranicum(NRIC 1541)等が挙げられる。なお、上記菌種名に関し、「NRIC」とは東京農業大学応用生物科学部菌株保存室が保存する菌株を意味し、「NISL」とは、財団法人野田産業科学研究所が保存する菌株を意味する。
これらの乳酸菌を使用すると、発酵により得られる豆乳発酵物中の粒度分布において粒径の大きいものがより少なく、粒径の小さいものが占める割合がより高いという特徴を有し、滑らかなクリーム状の物性を有する豆乳発酵物を安定的に製造することができ好ましい。また、粒径の大きいものの含量が一定値以下である豆乳発酵物及びそれを含有する均質組成物同士の比較においては、小さな粒径のものが占める割合がより多いもの、又は、平均粒径がより小さいものが一層滑らかな物性と優れた均質安定性を有するため、より好ましい。このような豆乳発酵物を生産可能な菌種としては、例えば、Leuconostoc pseudomesenteroides、Leuconostoc mesenteroides、Leuconostoc paramesenteroides等が挙げられ、より具体的には、Leuconostoc pseudomesenteroides(NISL7223)、Leuconostoc mesenteroides(NISL 7200)等が挙げられる。
(Lactic acid bacteria)
The soymilk fermented product of the present invention in which particles having a particle size of 80 μm or more in the particle size distribution are 10% or less can be obtained by adding lactic acid bacteria to soymilk and causing lactic acid fermentation to be performed. As the lactic acid bacterium used, any lactic acid bacterium can be used as long as it is capable of producing a fermented soymilk product having a particle size distribution of 80 μm or more and a particle size of 10% or less when inoculated and cultured in soy milk. is there. Examples include microorganisms belonging to the genus Leuconostoc. Specific examples of the species include Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides, Leuconostoc citreum, Leuconostoc dextranicum, and more specifically, for example, Leuconostoc mesenteroides (NISL 7219, NISL 7200, NRIC 1539), Leuconostoc NISL3 ), Leuconostoc citreum (NRIC 1579), Leuconostoc dextranicum (NRIC 1541), and the like. In addition, regarding the above bacterial species names, “NRIC” means a strain preserved by the Tokyo University of Agriculture, Department of Applied Biological Sciences, and the “NISL” means a strain preserved by the Noda Institute of Industrial Science. To do.
When these lactic acid bacteria are used, the particle size distribution in the fermented soymilk obtained by fermentation is characterized by the fact that there are fewer particles with a larger particle size and a higher proportion of particles with a smaller particle size. Fermented soymilk having physical properties can be stably produced, which is preferable. In addition, in the comparison between fermented soymilk having a large particle size content of a certain value or less and a homogeneous composition containing the same, those having a larger proportion of small particle size, or the average particle size is Smaller ones are more preferable because they have smoother physical properties and excellent homogeneous stability. Examples of bacterial species capable of producing such fermented soymilk include Leuconostoc pseudomesenteroides, Leuconostoc mesenteroides, Leuconostoc paramesenteroides, and more specifically, Leuconostoc pseudomesenteroides (NISL7223), Leuconostoc mesenteroides (NISL 7200) and the like. Can be mentioned.

(乳酸発酵)
豆乳の発酵には公知の方法を用いればよく、例えば、豆乳に糖類を添加し、そこに前述の乳酸菌を加えて発酵させ、豆乳中の粒子の粒径を小さくするために好適な培養条件下、例えば、30〜35℃で4時間以上の発酵を行わせ、適宜発酵物の粒度分布を測定しながら発酵させる方法が挙げられる。このようにして、粒度分布において80μm以上の粒子径を有する粒子が10%以下である豆乳発酵物を得ることができる。
(Lactic acid fermentation)
A well-known method may be used for fermentation of soy milk. For example, saccharides are added to soy milk, and the above-mentioned lactic acid bacteria are added thereto to ferment, and culture conditions suitable for reducing the particle size of particles in soy milk For example, the method of making it ferment for 4 hours or more at 30-35 degreeC, and making it ferment while measuring the particle size distribution of fermented material suitably is mentioned. In this manner, a soymilk fermented product having 10% or less of particles having a particle size of 80 μm or more in the particle size distribution can be obtained.

(豆乳発酵物の粒度分布)
本発明において、豆乳発酵物の粒度分布における80μm以上の粒子径を有する粒子の割合が10%以下であることは重要である。すなわち、粒度分布において80μm以上の粒子径を有する粒子の割合が10%を超える豆乳発酵物はざらついた食感が感じられ、均質安定性も劣る傾向を有するので好ましくない。また、粒度分布において80μm以上の粒子径を有する粒子の割合が10%を超える豆乳発酵物を水性調味液等と混合し均質組成物を製造した場合、例えばpH4.7以下の低pH条件下において前記均質化組成物が凝集、分離を起こす、または起こし易くなるという問題点を有するので好ましくない。
これに対し、80μm以上の粒子径を有する粒子の割合が10%以下である豆乳発酵物及びそれを含有する均質組成物は、特殊な水溶性ヘミセルロースを添加したり、豆乳をプロテアーゼで加水分解したりすることなく、pH4.7以下の低pH条件下でも組成物が凝集、分離しないという優れた均質安定性を有し、好ましい。さらに、80μm以上の粒子径を有する粒子の割合が10%以下である豆乳発酵物の中でも、小さな粒径のものが占める割合がより多いもの、又は、平均粒径がより小さいものは一層滑らかな物性と優れた均質安定性を有する。より好ましくは、70μm以上の粒子径を有する粒子の割合が10%以下である豆乳発酵物、さらに好ましくは、50μm以上の粒子径を有する粒子の割合が10%以下である豆乳発酵物、さらに好ましくは、20μm以上の粒子径を有する粒子の割合が10%以下である豆乳発酵物は、一層滑らかなクリーム状の物性と優れた均質安定性を有する。
なお、粒度分布の測定は、粒度分布測定装置、例えば「島津レーザー回析式粒度分布測定装置、SALD−2200−WJA1:V1.02」(島津製作所社製)を用いて測定することができる。
(Particle size distribution of fermented soymilk)
In the present invention, it is important that the proportion of particles having a particle size of 80 μm or more in the particle size distribution of the fermented soymilk is 10% or less. That is, fermented soymilk in which the proportion of particles having a particle size of 80 μm or more in the particle size distribution exceeds 10% is not preferable because it has a rough texture and tends to be inferior in homogeneous stability. Moreover, when a soymilk fermented product in which the proportion of particles having a particle size of 80 μm or more in the particle size distribution exceeds 10% is mixed with an aqueous seasoning liquid or the like to produce a homogeneous composition, for example, under a low pH condition of pH 4.7 or less The homogenized composition is not preferable because it has a problem that it causes aggregation or separation, or is likely to occur.
In contrast, fermented soymilk in which the proportion of particles having a particle diameter of 80 μm or more is 10% or less and a homogeneous composition containing the same are obtained by adding special water-soluble hemicellulose or hydrolyzing soymilk with a protease. Therefore, the composition has an excellent homogeneous stability that the composition does not aggregate and separate even under a low pH condition of pH 4.7 or lower. Furthermore, among the soymilk fermented products in which the proportion of particles having a particle diameter of 80 μm or more is 10% or less, those having a larger proportion of small particle size or those having a smaller average particle size are smoother. Has physical properties and excellent homogeneous stability. More preferably, the fermented soymilk product has a ratio of particles having a particle diameter of 70 μm or more is 10% or less, more preferably a fermented soymilk product having a ratio of particles having a particle diameter of 50 μm or more is 10% or less, more preferably Is a fermented soymilk in which the proportion of particles having a particle diameter of 20 μm or more is 10% or less, has a smoother creamy physical property and excellent homogeneous stability.
The particle size distribution can be measured using a particle size distribution measuring device, for example, “Shimadzu Laser Diffraction Particle Size Distribution Measuring Device, SALD-2200-WJA1: V1.02” (manufactured by Shimadzu Corporation).

前記の豆乳発酵物は、マヨネーズやドレッシングのような乳化液状調味料やデザート等の素材として摂食するのに好ましいクリーミーな(滑らかな)食感及びさわやかな酸味と豆乳由来のコクのある旨味を有し、また、すぐれた均質安定性を有している。そしてこの特性が本発明の豆乳発酵物含有均質組成物の濃厚な味とクリーミーな(滑らかな)食感、及び長期保存に耐え得る高い均質安定性に寄与する。従って、本発明の豆乳発酵物を、例えば従来の豆乳や豆乳発酵物に代えて、あるいは牛乳に代えて使用した場合には、良好な食感及び均質安定性を有する各種均質組成物を得ることができる。   The fermented soymilk has a creamy (smooth) texture that is preferable for eating as an emulsified liquid seasoning such as mayonnaise and dressing, and a dessert, and a refreshing sourness and a rich taste derived from soymilk. And has excellent homogeneous stability. This characteristic contributes to the rich taste and creamy (smooth) texture of the soymilk fermented product-containing homogeneous composition of the present invention and high homogeneity stability that can withstand long-term storage. Therefore, when the soymilk fermented product of the present invention is used in place of, for example, conventional soymilk or soymilk fermented product or in place of milk, various homogeneous compositions having good texture and homogeneous stability are obtained. Can do.

(豆乳発酵物含有均質組成物における豆乳発酵物の配合)
粒度分布において80μm以上の粒子径を有する粒子が10%以下である本発明の豆乳発酵物を、例えば、水性調味料等の原料と任意の配合で使用して、ドレッシングやマヨネーズ様調味料のような豆乳発酵物含有均質組成物を製造することができる。その場合、例えば、本発明の豆乳発酵物を前記豆乳発酵物含有均質組成物全量に対して5〜75%(w/w)の範囲で使用することが好ましい。豆乳発酵物の使用量が非常に少ない場合、例えば、5%(w/w)未満の場合は、本発明の豆乳発酵物が寄与する均質安定性を確保することが困難となる傾向がある。また、反対に豆乳発酵物の使用量が75%(w/w)を超える場合には、水性調味液の配合量に対する豆乳の風味が濃厚となり、酸味が不足する、豆乳の風味が強すぎるなど、調味料としての嗜好性にやや劣るものとなる傾向がある。これに対し、豆乳発酵物の使用量が5〜75%(w/w)の範囲にあるときには、調味料として良好な風味を有しつつ、良好な均質安定性を確保することが可能である。より好ましくは、豆乳発酵物の使用量は20〜70%(w/w)であり、その場合には、低pH条件下での均質安定性はより良好なものとなり、凝集、分離が起こりにくく、しかもその他の原材料との配合バランスがとれ良好な風味を有する調味料を得ることが可能となる。その範囲において、例えば、豆乳発酵物の使用量を20〜40%(w/w)とする配合は、凝集、分離が起こりにくく良好な風味を有するドレッシングタイプ調味料を製造するのに好適である。また、豆乳発酵物の使用量を35〜70%(w/w)とする配合は、例えば、澱粉等のいわゆる糊料の添加を低減あるいは省略してもマヨネーズ同様の粘度を有すると共に凝集、分離が起こりにくく良好な風味を有するマヨネーズ風調味料を製造するのに好適である。
(Composition of soymilk fermented product in a soymilk fermented product-containing homogeneous composition)
The fermented soymilk of the present invention in which the particle size distribution has a particle size of 80 μm or more and 10% or less of the present invention is used, for example, as a dressing or mayonnaise-like seasoning using a raw material such as an aqueous seasoning in any combination. Homogeneous soymilk-containing homogenous composition can be produced. In that case, for example, it is preferable to use the soymilk fermented product of the present invention in a range of 5 to 75% (w / w) with respect to the total amount of the soymilk fermented product-containing homogeneous composition. When the amount of fermented soymilk used is very small, for example, less than 5% (w / w), it tends to be difficult to ensure the homogeneous stability to which the fermented soymilk of the present invention contributes. On the other hand, if the amount of fermented soymilk used exceeds 75% (w / w), the soymilk's flavor with respect to the blending amount of the aqueous seasoning liquid becomes thick, sourness is insufficient, the soymilk's flavor is too strong, etc. , Tend to be slightly inferior in palatability as a seasoning. On the other hand, when the amount of fermented soymilk is in the range of 5 to 75% (w / w), it is possible to ensure good homogeneous stability while having a good flavor as a seasoning. . More preferably, the amount of fermented soymilk used is 20 to 70% (w / w), in which case the homogeneous stability under low pH conditions becomes better, and aggregation and separation hardly occur. In addition, it becomes possible to obtain a seasoning having a good blending balance with other raw materials and having a good flavor. In that range, for example, a blending amount of 20 to 40% (w / w) of the fermented soymilk is suitable for producing a dressing-type seasoning that has a good flavor that hardly causes aggregation and separation. . In addition, blending with a used amount of fermented soymilk of 35 to 70% (w / w), for example, has the same viscosity as mayonnaise and agglomerates and separates even if the addition of so-called paste such as starch is reduced or omitted. It is suitable for producing a mayonnaise-like seasoning having a good flavor.

(水性調味液)
調味料として好適な本発明の豆乳発酵物含有均質組成物を製造するために使用する水性調味液とは、任意の水性の調味液を意味し、例えば、米酢、穀物酢、果実酢、ワインビネガー等の醸造酢;濃口醤油、淡口醤油、白醤油等の醤油類;味醂、みりん風調味料等の甘味調味料;ワインや日本酒等の酒類;酢酸、乳酸、クエン酸、リンゴ酸、酒石酸等の有機酸溶液;食塩;グルタミン酸ナトリウム、イノシン酸ナトリウム、コハク酸ナトリウム等の化学調味料溶液;砂糖、水飴、蜂蜜等の甘味料溶液;清澄なだし汁あるいは動物又は植物蛋白質加水分解物等のアミノ酸調味料;発酵調味料、酵母エキス、魚介類エキス、野菜抽出物、海草エキス等のエキス類等の1種又は2種以上を含有する水性調味液が挙げられる。
(Aqueous seasoning liquid)
The aqueous seasoning liquid used to produce the soymilk fermented product-containing homogeneous composition of the present invention suitable as a seasoning means any aqueous seasoning liquid, for example, rice vinegar, grain vinegar, fruit vinegar, wine Brewing vinegar such as vinegar; soy sauces such as soy sauce, light soy sauce and white soy sauce; sweet seasonings such as miso and mirin-like seasonings; liquors such as wine and sake; acetic acid, lactic acid, citric acid, malic acid, tartaric acid, etc. Organic acid solution; salt; chemical seasoning solution such as sodium glutamate, sodium inosinate and sodium succinate; sweetener solution such as sugar, starch syrup and honey; amino acid seasoning such as clear soup stock or animal or plant protein hydrolysate An aqueous seasoning liquid containing one or more of fermented seasonings, yeast extracts, seafood extracts, vegetable extracts, seaweed extracts and other extracts.

(その他の原材料)
その他の原材料としては、一般的な乳化調味料、例えば、マヨネーズや各種乳化液状ドレッシング類に使用されている、食塩、糖類、味噌、辛子、胡椒、胡麻ペーストやにんにくペースト、ハーブ粉末、ナツメグ、セージ、タイム、シナモン、これらの抽出物等の香辛料、核酸系調味料、果汁、野菜や果実、種実類等、トマト、にんじん、タマネギ、にんにく、ゴマ、リンゴ、ゆず、オレンジ、モモ、イチゴ、パイナップル、ミカン、ブドウ等の加工処理物(ピューレ、ペースト、練状物、細断粒状物、磨砕物、粉末等)が挙げられる。なお必要により、加工澱粉や寒天、キサンタンガム、コーンスターチ、ペクチン等の各種安定剤や増粘剤を適量添加することができる。
(Other raw materials)
Other raw materials include general emulsified seasonings such as salt, sugar, miso, pepper, pepper, sesame paste, garlic paste, herb powder, nutmeg, sage used in mayonnaise and various emulsified liquid dressings. , Thyme, cinnamon, spices such as these extracts, nucleic acid seasonings, fruit juice, vegetables and fruits, seeds, etc., tomato, carrot, onion, garlic, sesame, apple, yuzu, orange, peach, strawberry, pineapple, Processed products such as oranges and grapes (purees, pastes, pastes, shredded granules, ground products, powders, etc.). If necessary, an appropriate amount of various stabilizers and thickeners such as processed starch, agar, xanthan gum, corn starch, and pectin can be added.

また、このような調味料の原料として、サラダオイル、コーンオイル、サフラワーオイル等の各種精製油脂の1種又は2種以上を、例えば、1〜70%使用してもよい。さらに、本発明の豆乳発酵物は、そのような精製油脂を添加しない場合でも、豆乳発酵物及び/又はその他の原料に由来するわずかな油脂分のみを利用して、良好な食感及び風味を有する均質安定性に優れた調味料の製造を可能にするため、精製油脂の添加による高カロリー化、高コレステロール化を抑制できる精製油脂無添加調味料の提供を可能にする。なお、本発明の豆乳発酵物は乳化力にも優れるため、均質乳化機等の特殊な装置を用いることなく、通常の混合装置中で攪拌することにより豆乳発酵物含有均質組成物を容易に得ることができる。また、原材料に精製油脂を添加する場合は、油脂以外の原材料を混合後、油脂を徐々に添加しながらコロイドミルなどで乳化を行うことにより、均質な豆乳発酵物含有調味料を容易に得ることができる。   Moreover, you may use 1 to 70% of 1 type, or 2 or more types of various refined fats and oils, such as salad oil, corn oil, safflower oil, as a raw material of such a seasoning. Further, the fermented soymilk of the present invention has a good texture and flavor using only a small amount of fat and oil derived from the fermented soymilk and / or other raw materials even when such refined fats and oils are not added. In order to make it possible to produce a seasoning excellent in homogeneous stability, it is possible to provide a seasoning with no added refined fats and oils that can suppress the increase in calories and cholesterol due to the addition of refined fats and oils. In addition, since the fermented soymilk of the present invention is also excellent in emulsifying power, a soymilk fermented material-containing homogeneous composition can be easily obtained by stirring in a normal mixing device without using a special device such as a homogenous emulsifier. be able to. In addition, when adding refined fats and oils to raw materials, after mixing raw materials other than fats and oils, emulsifying with a colloid mill etc. while gradually adding fats and oils, it is easy to obtain a homogeneous soymilk-containing fermented seasoning Can do.

(本発明の豆乳発酵物含有均質組成物のpH)
前記豆乳発酵物、前記水性調味液、及び必要に応じその他の原材料を混合して均質化し、有機酸又は食酢を用いてpHを3.0〜4.7に調整して本発明の豆乳発酵物含有均質組成物を調製することができる。本発明の豆乳発酵物含有均質組成物においてpHを4.7以下となるように調整することは重要である。pHが4.7を超える場合、得られる豆乳発酵物含有均質組成物には酸味が不足し、調味料としての嗜好性に劣るものとなる。pHが3.0を下回る場合には、得られる豆乳発酵物含有均質組成物は酸味が強すぎることによって調味料としての嗜好性に劣るものとなる。
(PH of the soymilk fermented product-containing homogeneous composition of the present invention)
The soymilk fermented product, the aqueous seasoning liquid, and other ingredients as necessary are mixed and homogenized, and the pH is adjusted to 3.0 to 4.7 using an organic acid or vinegar, and the soymilk fermented product of the present invention is used. Containing homogeneous compositions can be prepared. In the soymilk fermented product-containing homogeneous composition of the present invention, it is important to adjust the pH to be 4.7 or less. When the pH exceeds 4.7, the soymilk fermented product-containing homogeneous composition obtained lacks sourness and is inferior in palatability as a seasoning. When pH is less than 3.0, the soymilk fermented material containing homogeneous composition obtained becomes inferior in palatability as a seasoning because the sourness is too strong.

以下、実施例により、本発明をさらに具体的に説明する。ただし、本発明の技術的範囲は、それらの例により何ら限定されるものではない。   Hereinafter, the present invention will be described more specifically with reference to examples. However, the technical scope of the present invention is not limited by these examples.

1.粒度分布において80μm以上の粒子径を有する粒子が10%以下である豆乳発酵物(本発明)の調製
1)豆乳の調製
大豆固形分14%(w/v)の無調整豆乳に対して、無菌フィルターろ過済みの20%シュークロース溶液を等量加えることで、終濃度において10%(w/v)シュークロースを含有する大豆固形分7%(w/v)の豆乳(以下、「発酵前の豆乳」と称する)を調製した。調製した上記豆乳を滅菌済みのカップ等の容器に充填した。
2)乳酸菌の調製
表1記載のLeuconostoc属に属する乳酸菌を、乳酸菌用調製培地(1.0%大豆ペプトン、0.5%酵母エキス、1.0%グルコース、0.5%酢酸ナトリウム)にて30℃、20時間培養した。培養液10mlを遠心分離(3,000g×5min)して菌体を回収した。
3)発酵
上記2)で回収した各種菌体を、上記1)に記載の調製した豆乳に全量添加し、30℃、24時間恒温状態にて発酵を行い、豆乳発酵物を得た。
1. Preparation of soymilk fermented product (invention) having particles having a particle size of 80 μm or more in the particle size distribution of 10% or less 1) Preparation of soymilk Aseptic to unadjusted soymilk having a soy solid content of 14% (w / v) By adding an equal amount of 20% sucrose solution that has been filtered, soymilk (hereinafter referred to as “before fermentation”) containing 7% (w / v) soybean solids containing 10% (w / v) sucrose in the final concentration. (Referred to as “soy milk”). The prepared soymilk was filled in a container such as a sterilized cup.
2) Preparation of lactic acid bacteria Lactic acid bacteria belonging to the genus Leuconostoc listed in Table 1 were prepared in a lactic acid bacteria preparation medium (1.0% soybean peptone, 0.5% yeast extract, 1.0% glucose, 0.5% sodium acetate). The cells were cultured at 30 ° C. for 20 hours. 10 ml of the culture solution was centrifuged (3,000 g × 5 min) to recover the cells.
3) Fermentation All the bacterial cells collected in 2) above were added to the soymilk prepared in 1) above, and fermentation was performed at 30 ° C. for 24 hours to obtain a fermented soymilk.

2.粒度分布において80μm以上の粒子径を有する粒子が10%以上である豆乳発酵物(比較例)の調製
ヨーグルト生産用の乳酸菌として知られている表1記載のLactobacillus属に属する乳酸菌を用いて、上記3)に記載の方法と同様に発酵を行い、比較例の豆乳発酵物を得た。
2. Preparation of Fermented Soymilk (Comparative Example) with 10% or more of Particles having a Particle Size of 80 μm or More in Particle Size Distribution Using the lactic acid bacteria belonging to the genus Lactobacillus in Table 1 known as lactic acid bacteria for yogurt production, Fermentation was performed in the same manner as described in 3) to obtain a soymilk fermented product of a comparative example.

3.本発明の豆乳発酵物及び比較例の豆乳発酵物の評価
上記により得られた各豆乳発酵物の粒度分布を「島津レーザー回析式粒度分布測定装置、SALD−2200−WJA1:V1.02」を用いて測定した。得られたデータのうち代表的な数例の菌株に関する粒度分布のプロファイルを図1に示す。
図1に示される通り、比較例であるLactobacillus属の微生物を用いて発酵を行った豆乳発酵物においては、粒径が5μm以下の粒子がほとんどなく、粒径200μmを超える大きさの粒子も多く含まれ、80μmを超える粒子が占める割合は10%を大きく上回る。これに対し、本発明のLeuconostoc属の微生物を用いて発酵を行った豆乳発酵物には粒径200μmを超える大きさの粒子はみられず、粒径80μmを超える大きさの粒子が占める割合はいずれも10%以下であった。Leuconostoc mesenteroides(NRIC 1539)を用いた豆乳発酵物では、約30μmをピークとする粒径約5μmから約200μm以下の粒子を含み、粒径80μmを超える大きさの粒子が占める割合は10%であった。また、Leuconostoc mesenteroides(NISL 7200)の場合は、約20μmをピークとする粒径約4μmから約100μmの粒子を含み、粒径80μmを超える大きさの粒子はほとんど存在しなかった。さらに、Leuconostoc pseudomesenteroides(NISL7223)の場合は、含まれる粒子の粒径はさらに小さく、約15μmを超える粒径の粒子はみられなかった。
表1に、各豆乳発酵物の粒度分布プロファイルに基づく90%Dの値を示す。90%Dの値とは、粒度分布プロファイルにおいて粒子径の小さい方から90%に含まれる粒子の最大径を意味する。例えば、90%Dの値が80である場合、この豆乳発酵物における粒子の小さい方から90%に含まれる粒子の最大径は80μmであり、すなわちこの豆乳発酵物には粒子径が80μm以上である粒子を全体の10%含むことを意味する。
表1において、Lactobacillus属に属する比較例の各種乳酸菌を用いた豆乳発酵物では、いずれも90%Dの値が100を上回り、粒子径が100μmを超える大きな粒子が全体の10%を占め、図1からも明らかであるように、粒子径が80μm以上である粒子の割合としては10%を大きく超えることがわかる。これに対し、Leuconostoc属に属する本発明の各種乳酸菌を用いた場合には、90%Dの値がいずれも80以下であり、粒度分布において80μm以上の粒子径を有する粒子の割合が10%以下であることがわかる。特に、Leuconostoc mesenteroides(NISL 7200)の場合は、90%Dの値は40であり、40μm以上の大きさの粒子が10%以下となる。さらに、Leuconostoc pseudomesenteroides(NISL7223)の場合には、90%Dの値が9であり、10μm以上の大きさの粒子は10%以下にすぎず、全体の粒子径が非常に小さいことがわかる。図1及び表1の結果から、使用する乳酸菌の菌株の相違により、粒度分布において80μm以上の粒子径を有する粒子の割合が大きく異なることがわかる。
3. Evaluation of Soymilk Fermented Product of the Present Invention and Comparative Example Soymilk Fermented Product The particle size distribution of each soymilk fermented product obtained as described above is “Shimadzu Laser Diffraction Particle Size Distribution Measuring Device, SALD-2200-WJA1: V1.02.” And measured. FIG. 1 shows the particle size distribution profile for several representative strains of the obtained data.
As shown in FIG. 1, the fermented soymilk fermented using a microorganism of the Lactobacillus genus as a comparative example has few particles having a particle size of 5 μm or less, and many particles having a particle size exceeding 200 μm. The proportion of particles that are included and exceed 80 μm greatly exceeds 10%. On the other hand, the fermented soymilk fermented using the microorganism of the genus Leuconostoc of the present invention does not show particles having a particle size exceeding 200 μm, and the proportion of particles having a particle size exceeding 80 μm is All were 10% or less. In fermented soymilk using Leuconostoc mesenteroides (NRIC 1539), particles containing particles with a particle size of about 5 μm to about 200 μm or less with a peak at about 30 μm are occupied by 10%. It was. In the case of Leuconostoc mesenteroides (NISL 7200), particles having a particle size of about 4 μm to about 100 μm with a peak at about 20 μm were included, and there were almost no particles having a particle size exceeding 80 μm. Furthermore, in the case of Leuconostoc pseudomesenteroides (NISL7223), the particle size of the contained particles was even smaller, and no particles with a particle size exceeding about 15 μm were observed.
Table 1 shows a value of 90% D based on the particle size distribution profile of each soymilk fermented product. The value of 90% D means the maximum diameter of particles contained in 90% from the smaller particle diameter in the particle size distribution profile. For example, when the value of 90% D is 80, the maximum diameter of particles contained in 90% from the smallest particle in this soymilk fermented product is 80 μm, that is, the soymilk fermented product has a particle size of 80 μm or more. It means to contain 10% of all particles.
In Table 1, in the fermented soymilk using various lactic acid bacteria of Comparative Examples belonging to the genus Lactobacillus, the 90% D value exceeds 100, and large particles with a particle diameter exceeding 100 μm account for 10% of the total. As is clear from FIG. 1, the ratio of particles having a particle diameter of 80 μm or more greatly exceeds 10%. On the other hand, when various lactic acid bacteria of the present invention belonging to the genus Leuconostoc are used, the value of 90% D is 80 or less, and the ratio of particles having a particle size of 80 μm or more in the particle size distribution is 10% or less. It can be seen that it is. In particular, in the case of Leuconostoc mesenteroides (NISL 7200), the value of 90% D is 40, and particles having a size of 40 μm or more are 10% or less. Further, in the case of Leuconostoc pseudomesenteroides (NISL7223), the value of 90% D is 9, and the particle size of 10 μm or more is only 10% or less, and the entire particle diameter is very small. From the results in FIG. 1 and Table 1, it can be seen that the proportion of particles having a particle size of 80 μm or more in the particle size distribution varies greatly depending on the strain of lactic acid bacteria used.

Figure 2008161070
Figure 2008161070

4.豆乳発酵物含有均質組成物及び豆乳含有均質組成物の調製
上記3.で得られた各種豆乳発酵物、食酢(5%(w/w)酢酸濃度のリンゴ酢)、及び水を70g、20g、及び110gの配合割合で混合し、豆乳発酵物含有均質組成物(本発明及び比較例)を得た。また、対照として上記1.1)に記載の「発酵前の豆乳」を用いる以外は全く同様の方法を用いて豆乳含有均質組成物(対照)を得た。
4). 2. Preparation of fermented soymilk-containing homogeneous composition and soymilk-containing homogeneous composition The soymilk fermented product obtained in 1), vinegar (apple vinegar with 5% (w / w) acetic acid concentration), and water were mixed in a mixing ratio of 70 g, 20 g, and 110 g, and the soymilk fermented product-containing homogeneous composition (this Invention and Comparative Example) were obtained. Further, a soymilk-containing homogeneous composition (control) was obtained using the same method except that the “pre-fermented soymilk” described in 1.1) above was used as a control.

5.本発明豆乳発酵物含有均質組成物及び比較例の豆乳発酵物含有均質組成物の均質安定性評価
上記の本発明、比較例及び対照の豆乳発酵物含有均質組成物を各10ml分取し、15ml容遠心分離用試験管に入れ、5℃、20分間、1,000rpmにて遠心後、分離状態を目視観察すると共に、分離された透明な上層の液量を測定した。
また、上層以外の不透明部分(沈殿、ふわふわとした中間層、及び遠心分離前の乳化状態と同様の液状部分を含む)を下層として、その量を測定した。
豆乳発酵物含有均質組成物全体に対する上層の割合(x%)、及び下層の割合((100−x)%)を算出し、分離状態(均質安定性)の尺度とした。そして上層の割合(x%)が0〜10%のものを「◎(分離せず)」、10〜20%のものを「○(やや分離)」、及び20%以上のものを「×(分離)」として、均質安定性評価結果を表1に示す。なお、調製された豆乳発酵物含有均質組成物又は豆乳含有均質組成物のpHはそれぞれ、3.8(本発明)、3.8(比較例)及び4.2(対照)であった。
5. Homogeneous stability evaluation of the soymilk fermented product-containing homogeneous composition of the present invention and the soymilk fermented product-containing homogeneous composition of the comparative example 10 ml each of the above-mentioned present invention, comparative example and control soymilk fermented product-containing homogeneous composition was taken, and 15 ml The sample was placed in a test tube for centrifugal separation, centrifuged at 1,000 rpm at 5 ° C. for 20 minutes, and the separated state was visually observed, and the liquid amount of the separated transparent upper layer was measured.
Further, the amount of the opaque portion other than the upper layer (including a precipitate, a fluffy intermediate layer, and a liquid portion similar to the emulsified state before centrifugation) was measured as the lower layer, and the amount thereof was measured.
The ratio of the upper layer (x%) and the ratio of the lower layer ((100-x)%) to the whole soymilk fermented product-containing homogeneous composition were calculated and used as a measure of the separation state (homogeneous stability). And the ratio (x%) of the upper layer is 0 to 10% “◎ (not separated)”, 10 to 20% “O (slightly separated)”, and 20% or more “× ( Table 1 shows the results of homogeneous stability evaluation. In addition, pH of the prepared soymilk fermented material containing homogeneous composition or the soymilk containing homogeneous composition was 3.8 (this invention), 3.8 (comparative example), and 4.2 (control), respectively.

表1に記載の通り、「粒度分布において80μm以上の粒子径を有する粒子が10%以上である豆乳発酵物」を用いた豆乳発酵物含有均質組成物(比較例)においては固液分離が認められる欠点を有していた。なお、乳酸発酵処理しない「発酵前の豆乳」を用いた豆乳発酵物含有均質組成物(対照)では、さらに固液分離が激しく、均質安定性が非常に劣っていた。
これに対し、「粒度分布において80μm以上の粒子径を有する粒子が10%以下である豆乳発酵物」を用いた豆乳発酵物含有均質組成物は、固液分離は全く起こらないかほとんど起こらず、均質安定性が高いことがわかる。
以上の結果から、粒度分布において80μm以上の粒子径を有する粒子が10%以上である豆乳発酵物を水性調味液と混合して豆乳発酵物含有均質組成物を調製するときには、pH4.7以下の低pH条件下となる場合でも豆乳発酵物含有均質組成物の凝集、分離が起こらず均質安定性に優れていることがわかる。
As shown in Table 1, solid-liquid separation was observed in the soymilk fermented product-containing homogeneous composition (comparative example) using “fermented soymilk having 10% or more particles having a particle size of 80 μm or more in the particle size distribution”. Had the disadvantages. In addition, in the soymilk fermented product-containing homogeneous composition (control) using “pre-fermented soymilk” that was not subjected to lactic acid fermentation treatment, solid-liquid separation was further severe and the homogeneous stability was very poor.
On the other hand, the soymilk fermented product-containing homogeneous composition using “fermented soymilk having 10% or less of particles having a particle size of 80 μm or more in the particle size distribution” causes little or no solid-liquid separation, It can be seen that the homogeneous stability is high.
From the above results, when preparing a soymilk fermented product-containing homogeneous composition by mixing a soymilk fermented product having a particle size distribution of particles having a particle size of 80 μm or more of 10% or more with an aqueous seasoning liquid, the pH is 4.7 or less. It can be seen that even under low pH conditions, the homogeneous composition containing soymilk fermented product does not aggregate and separate, and is excellent in homogeneous stability.

実施例1に記載の各種Leuconostoc属の微生物を使用して得られた豆乳発酵物を使用して、以下の配合割合にて、pH4.3の豆乳発酵物含有均質組成物(マヨネーズ風調味料)を製造した。
配合割合
・粒度分布において80μm以上の
粒子径を有する粒子が10%以下
である豆乳発酵物 :300g
・水飴 :230g
・食塩 :34g
・リンゴ酢(酢酸5%(w/w)) :80ml
・加工澱粉 :28g
・小麦発酵調味料(粉末) :10g
・香辛料(マスタード) :2g
・黄色素(着色料) :0.5g
・水 :合計容量が1000mlとなるように添加
合計値 :1000ml

得られた豆乳発酵物含有均質組成物は良好な均質安定性を示し、食感は滑らかで風味も良好であった。また、この豆乳発酵物含有均質組成物を200ml容ガラス製密封容器に入れ、室温にて4ヶ月間静置した時も分離は観察されず、均質性が維持されていた。
Using the soymilk fermented product obtained using various microorganisms of the genus Leuconostoc described in Example 1, a soymilk fermented product-containing homogeneous composition (mayonnaise-like seasoning) having a pH of 4.3 at the following blending ratio: Manufactured.
Mixing ratio-Fermented soymilk with 300% or less particles having a particle size of 80 μm or more in the particle size distribution: 300 g
・ Water tank: 230g
・ Salt: 34g
・ Apple vinegar (acetic acid 5% (w / w)): 80ml
・ Processed starch: 28g
・ Wheat fermented seasoning (powder): 10g
・ Spice (Mustard): 2g
・ Yellow dye (coloring agent): 0.5g
-Water: added so that the total volume is 1000 ml. Total value: 1000 ml

The obtained homogenous composition containing fermented soymilk showed good homogeneity stability, and the texture was smooth and the flavor was good. Moreover, even when this soymilk fermented product-containing homogeneous composition was placed in a 200 ml glass sealed container and allowed to stand at room temperature for 4 months, no separation was observed and the homogeneity was maintained.

実施例1で得られた調整豆乳及び豆乳発酵物の粒度分布である。2 is a particle size distribution of the prepared soymilk and fermented soymilk obtained in Example 1. FIG.

Claims (4)

豆乳に乳酸菌を接種培養して得られる豆乳発酵物であって、その粒度分布において80μm以上の粒子径を有する粒子が10%以下であることを特徴とする豆乳発酵物。   A soymilk fermented product obtained by inoculating and cultivating lactic acid bacteria in soymilk, wherein the particle size distribution has a particle size of 80 μm or more and a particle size of 10% or less. 乳酸菌がLeuconostoc属に属する微生物である請求項1に記載の豆乳発酵物。   The fermented soymilk according to claim 1, wherein the lactic acid bacteria are microorganisms belonging to the genus Leuconostoc. 豆乳に乳酸菌を接種培養して得られる豆乳発酵物であって、その粒度分布において80μm以上の粒子径を有する粒子が10%以下である豆乳発酵物と水性調味液とを含有し、pHが3.0〜4.7以下である豆乳発酵物含有均質組成物。   A soymilk fermented product obtained by inoculating and cultivating lactic acid bacteria in soymilk, comprising a soymilk fermented product having a particle size distribution of 80 μm or more and a particle size distribution of 10% or less and an aqueous seasoning solution, and having a pH of 3 A homogenous composition containing a fermented soymilk that is 0.0 to 4.7 or less. 豆乳に乳酸菌を接種培養して得られる豆乳発酵物であって、その粒度分布において80μm以上の粒子径を有する粒子が10%以下である豆乳発酵物を、豆乳発酵物含有均質組成物全量に対して5〜75%(w/w)含有することを特徴とする請求項3に記載の豆乳発酵物含有均質組成物。   A soymilk fermented product obtained by inoculating and cultivating lactic acid bacteria in soymilk, wherein the soymilk fermented product having a particle size distribution of 80 μm or more in the particle size distribution is 10% or less with respect to the total amount of the soymilk fermented product-containing homogeneous composition The soymilk fermented product-containing homogeneous composition according to claim 3, comprising 5 to 75% (w / w).
JP2006351102A 2006-12-27 2006-12-27 Soymilk fermented product, and homogeneous composition containing the same Pending JP2008161070A (en)

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JPH03201944A (en) * 1989-03-07 1991-09-03 Unilever Nv Fermented soybean milk and production thereof
JPH10229841A (en) * 1997-02-21 1998-09-02 Yakult Honsha Co Ltd Lipid metabolism improving agent, and food containing the same
JP2000093083A (en) * 1998-09-21 2000-04-04 Fuji Oil Co Ltd Production of lactic fermented soya milk
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7282285B1 (en) * 2023-01-27 2023-05-26 キユーピー株式会社 liquid seasoning

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