JP5079654B2 - Vegetable beverage composition - Google Patents

Vegetable beverage composition Download PDF

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JP5079654B2
JP5079654B2 JP2008251980A JP2008251980A JP5079654B2 JP 5079654 B2 JP5079654 B2 JP 5079654B2 JP 2008251980 A JP2008251980 A JP 2008251980A JP 2008251980 A JP2008251980 A JP 2008251980A JP 5079654 B2 JP5079654 B2 JP 5079654B2
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carrot
fermentation
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beverage composition
juice
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JP2010081818A (en
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雄彦 佐藤
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Sunstar Inc
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本技術は、ニンジン搾汁が有する特有の風味を改善し、かつ加熱処理された際の特異臭の発生を抑制させた野菜飲料組成物に関する。   The present technology relates to a vegetable beverage composition that improves the peculiar flavor of carrot juice and suppresses the generation of a specific odor when heat-treated.

近年、日常の食生活において野菜を摂取することにより、多くの健康上のメリットが得られることが明らかになってきている。特に、野菜に多く含まれるビタミン、ミネラル、食物繊維などの栄養素の摂取や、生活習慣を起因とする疾病の発症予防の観点では重要な要因の一つとして挙げられており、特に、ニンジンには優れた機能が見出されていることから、ニンジン搾汁を含有した野菜飲料組成物が多く発売されている。   In recent years, it has become clear that many health benefits can be obtained by taking vegetables in daily eating habits. In particular, it is listed as one of the important factors in terms of intake of nutrients such as vitamins, minerals, and dietary fiber that are abundant in vegetables, and prevention of the onset of diseases caused by lifestyle habits. Since an excellent function has been found, many vegetable beverage compositions containing carrot juice have been put on the market.

しかし、ニンジン搾汁にはニンジン特有の風味が強いだけでなく、搾汁時や飲料生産時などにおける加熱処理により特異な加熱臭が付加されるため、ニンジン搾汁を配合した野菜飲料組成物は消費者の嗜好性が悪いという欠点が存在していた。そのため、ニンジン搾汁のニンジン特有の風味や加熱処理による特異な加熱臭の解消する方法の開発が望まれていた。   However, carrot juice not only has a carrot-specific flavor, but also has a unique heating odor due to heat treatment at the time of juice extraction or beverage production, so the vegetable beverage composition containing carrot juice is There was a drawback that consumer preference was bad. Therefore, development of the method of eliminating the peculiar carrot flavor of carrot juice and the unique heating odor due to heat treatment has been desired.

そこで、ニンジンに乳酸菌醗酵を行うことにより、ニンジン特有の風味を解消するという提案がなされている。例えば、人参を破砕後に搾汁した人参汁をラクトバチルス属菌やストレプトコッカス属菌を用いた乳酸菌醗酵を利用して製造する野菜飲料(特許文献1)、ラクトバチルス・プランタラムを用いた乳酸菌醗酵を行なうことでニンジンの不快臭味を改善する方法(特許文献2)、エンテロコッカス属菌を用いた乳酸菌醗酵を行なうことで緑黄色野菜の風味を改善する方法(特許文献3)などが提案されている。しかし、何れの方法もニンジン搾汁のニンジン特有の風味の改善効果は十分でなく、また加熱処理による特異な加熱臭の抑制についての提案はまったくなされていないため、未だ問題解決には至っていない。
特開平10−201455号公報 特開平05−084065号公報 特開平07−170933号公報
Then, the proposal of canceling the flavor peculiar to a carrot by making lactic acid bacteria fermentation to a carrot has been made. For example, vegetable drinks produced by using lactic acid bacteria fermentation using Lactobacillus or Streptococcus genus juice obtained by crushing ginseng after crushing ginseng (Patent Document 1), lactic acid bacteria fermentation using Lactobacillus plantarum There have been proposed a method for improving the unpleasant odor of carrots (Patent Document 2), a method for improving the flavor of green-yellow vegetables by performing lactic acid bacteria fermentation using Enterococcus spp. However, none of these methods has an adequate effect of improving the flavor unique to carrots of carrot juice, and no proposal has been made for the suppression of specific heating odors by heat treatment, so the problem has not yet been solved.
JP-A-10-2014455 Japanese Patent Laid-Open No. 05-084065 JP 07-170933 A

ニンジン特有の風味を改善し、かつ加熱処理を行った場合でも加熱臭などの好ましくない風味の発生を抑制した嗜好性の高いニンジン搾汁を含有する野菜飲料組成物を提供することを課題とする。   An object of the present invention is to provide a vegetable beverage composition containing carrot juice having a high palatability that improves the flavor unique to carrots and suppresses the occurrence of undesirable flavors such as heated odors even when heat treatment is performed. .

ビフィズス菌を用いて醗酵処理したニンジン破砕物とニンジン搾汁を含有させることにより、ニンジン特有の風味が改善され、かつ加熱処理による特異臭の発生を抑制し、嗜好性の高い野菜飲料組成物ができることを見出した。すなわち
項1 ニンジン破砕物のビフィズス菌醗酵物およびニンジン搾汁を配合することを特徴とする野菜飲料組成物。
項2 さらに、ニンジン破砕物を配合することを特徴とする項1に記載の野菜飲料組成物。
項3 ビフィズス菌が、ビフィドバクテリウム・ロンガムであることを特徴とする項1または項2の何れかに記載の野菜飲料組成物。
項4 さらに、糖酸比が17〜27になるように柑橘類の果汁を配合させることを特徴とする項1〜3の何れかに記載の野菜飲料組成物。
を提供するものである。
By including carrot crushed fermented with bifidobacteria and carrot juice, the flavor unique to carrots is improved, and the generation of a specific odor due to heat treatment is suppressed, and a highly palatable vegetable beverage composition is produced. I found out that I can do it. That is, the vegetable drink composition characterized by mix | blending the bifidobacteria fermented product of carrot crushed material, and carrot juice.
Item 2. The vegetable beverage composition according to Item 1, further comprising a carrot crushed product.
Item 3. The vegetable beverage composition according to Item 1 or 2, wherein the Bifidobacterium is Bifidobacterium longum.
Item 4. The vegetable beverage composition according to any one of Items 1 to 3, further comprising citrus juice so that the sugar acid ratio is 17 to 27.
Is to provide.

本発明によって、ニンジン搾汁特有の風味を改善し、かつ加熱処理による特異臭の発生を抑制することで嗜好性の高い野菜飲料組成物を提供することが出来る。   By this invention, the vegetable drink composition with high palatability can be provided by improving the flavor peculiar to carrot juice and suppressing generation | occurrence | production of the specific smell by heat processing.

本発明で用いるニンジンの使用部位は、野菜全体、葉部、根部などが挙げられ、特に限定されるものではないが、根部を主に使用することが好ましい。また、破砕処理や搾汁処理を行う際の前処理として、へたの切断、剥皮等の除去、有用部分の選択などを行ってから使用することもできる。   The use site of the carrot used in the present invention includes whole vegetables, leaves, roots and the like, and is not particularly limited, but it is preferable to mainly use the roots. Moreover, as pre-processing at the time of performing a crushing process or a squeezing process, it can also be used after performing a cutting | disconnection of a spatula, removal of peeling, etc., selection of a useful part, etc.

本発明で用いる破砕物とは、ニンジンを破砕処理によりピューレ状やペースト状にしたものをいう。具体的には、必要に応じて破砕処理する前にニンジン由来の酵素を失活させるための加熱処理(ブランチングと呼ばれる)を行った後に、ハンマークラッシャー、電動ミル、コミトロール、マスコロイダー、フードプロセッサー、パルパーフィニッシャー等の破砕機や裏ごし機を用いて粉砕し、粉砕物を得ることができる。好ましいニンジン破砕物は固形分が50重量%以上のものである。ここで言う固形分とは、ニンジン粉砕物のパルプ量を表す指標である。固形分の測定は、果汁飲料の不溶性固形分分析法を使用して行う。すなわち試料10mlを遠沈管に採取し、ローター半径14.5cmの遠心分離機を用いて、毎分3000回転、10分間遠心分離を行う。ここでは、分析精度を高めるため、上清部と沈殿部を分けて重量測定し、沈殿部分の重量パーセントを測定する。ニンジン破砕物は、流動性が悪いので、精製水により正確に2倍希釈して均一分散した試料を遠心分離し、沈殿物の重量%を2倍して固形分とする。   The crushed material used in the present invention refers to a carrot made into a puree or paste by crushing treatment. Specifically, after performing heat treatment (called blanching) to inactivate the carrot-derived enzyme before crushing as necessary, hammer crusher, electric mill, comitorol, mascolloider, hood A pulverized product can be obtained by pulverization using a crusher such as a processor or a pulper finisher or a back slicer. A preferable carrot crushed material has a solid content of 50% by weight or more. The solid content mentioned here is an index representing the pulp amount of the carrot pulverized product. The solid content is measured using an insoluble solid content analysis method for fruit juice beverages. That is, 10 ml of a sample is collected in a centrifuge tube and centrifuged at 3000 rpm for 10 minutes using a centrifuge with a rotor radius of 14.5 cm. Here, in order to improve the analysis accuracy, the supernatant part and the precipitation part are separately weighed, and the weight percentage of the precipitation part is measured. Since the carrot pulverized product has poor fluidity, the sample that has been diluted twice with purified water and homogeneously dispersed is centrifuged, and the weight percentage of the precipitate is doubled to obtain a solid content.

本発明で用いるビフィズス菌は、グラム陽性無芽胞桿菌のビフィドバクテリウム属をいう。具体的には、ビフィドバクテリウム・ロンガム(Bifidobacterium longum)、ビフィドバクテリウム・ブレーベ(Bifidobacterium breve)、ビフィドバクテリウム・ビフィダム(Bifidobacterium bifidum)、ビフィドバクテリウム・インファンティス(Bifidobacterium infantis)、ビフィドバクテリウム・カテヌラタム(Bifidobakuterium catenulatum)、ビフィドバクテリウム・アドレセンティス(Bifidobacterium adolescentis)、ビフィドバクテリウム・シュードロンガム(Bifidobacterium pseudolongum)、ビフィドバクテリウム・アニマリス(Bifidobacterium animalis)などが挙げられる。この中でもビフィドバクテリウム・ロンガム(Bifidobacterium longum)が最も好ましい。これらのビフィドバクテリウム属の桿菌は、単独もしくは複数の菌を組み合わせて用いることができる。   The bifidobacteria used in the present invention refers to the genus Bifidobacterium which is a gram-positive sporeless gonococcus. Specifically, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium infantis , Bifidobakuterium catenulatum, Bifidobacterium adolescentis, Bifidobacterium pseudolongum, Bifidobacterium animalis, etc. It is done. Among these, Bifidobacterium longum is most preferable. These Bifidobacterium spp. Can be used alone or in combination of a plurality of bacteria.

本発明に用いるビフィズス菌醗酵したニンジン破砕物は、以下に例示する醗酵方法などを行うことにより得ることができる。   The carrot crushed fermented bifidobacteria used in the present invention can be obtained by performing the fermentation method exemplified below.

ニンジン破砕物は、他の成分を混合することなくビフィズス菌醗酵を行うことが好ましい。しかし、粘度が高いなどの理由で醗酵処理に支障を生じる場合には、予め野菜搾汁、果汁、水などの液体成分を添加しても良い。これら液体成分を添加する場合は、添加後の破砕物の不溶性固形分量が50重量%以上となる範囲内で添加量を設定することが好ましい。   The carrot crushed product is preferably subjected to bifidobacteria fermentation without mixing other components. However, when the fermentation process is hindered due to high viscosity, liquid components such as vegetable juice, fruit juice, and water may be added in advance. When adding these liquid components, it is preferable to set the addition amount within a range in which the insoluble solid content of the crushed material after the addition is 50% by weight or more.

ビフィズス菌醗酵は、ニンジン破砕物にビフィズス菌のスターターを接種することで開始する。スターターは通常使用される方法で得られるものを使用できる。接種時のニンジン破砕物の温度は30〜43℃の範囲であれば特に制限されるものではないが、35〜42℃が好ましく、36〜40℃がもっとも好ましい。醗酵時のニンジン破砕物の温度は、18〜42℃とする。中でも18〜33℃の温度領域で醗酵させることが好ましく、18〜28℃の温度領域がもっとも好ましい。その理由は、ビフィズス菌の至適醗酵温度である36〜42℃(ビフィズス菌の科学、p164、初版第一刷、(株)食品化学新聞社)より低い18〜33℃で醗酵を行なうことにより、通常の条件で醗酵を行った場合とは異なった風味が付加され、この付加される醗酵風味により、ニンジン特有の風味を改善したり、加熱処理に伴う特異臭の発生を抑制したりする効果がさらに高くなるからである。ビフィズス菌醗酵の処理時間は醗酵時の温度条件などにより適宜調整されるが、通常6〜30時間であり、好ましくは8〜26時間である。   The bifidobacteria fermentation starts by inoculating a starter of bifidobacteria on the carrot crushed material. A starter obtained by a commonly used method can be used. Although it will not restrict | limit especially if the temperature of the carrot crushed material at the time of inoculation is the range of 30-43 degreeC, 35-42 degreeC is preferable and 36-40 degreeC is the most preferable. The temperature of the carrot crushed product during fermentation is 18 to 42 ° C. Among them, it is preferable to ferment in a temperature range of 18 to 33 ° C, and a temperature range of 18 to 28 ° C is most preferable. The reason for this is that the fermentation is carried out at 18 to 33 ° C., which is lower than the optimum fermentation temperature of Bifidobacterium, 36 to 42 ° C. (Science of Bifidobacterium, p164, first edition, first edition, Food Chemical Newspaper Co., Ltd.). The addition of a different flavor than when fermented under normal conditions, the effect of improving the flavor unique to carrots and suppressing the generation of a specific odor associated with heat treatment, by this added fermentation flavor This is because it becomes even higher. The treatment time for bifidobacteria fermentation is appropriately adjusted depending on the temperature conditions at the time of fermentation, but is usually 6 to 30 hours, and preferably 8 to 26 hours.

ビフィズス菌醗酵の終点は、破砕物の乳酸酸度で判断する。すなわち破砕物の乳酸酸度が0.35〜0.85重量%、好ましくは乳酸酸度0.35〜0.65重量%の範囲にあるときに醗酵を終了する。また、醗酵の進行を完全に停止させるために、醗酵終了後にできる限り速やかに加熱処理若しくは凍結処理を行うことが好ましい。このうち、特に冷凍処理を行うことが好ましい。   The end point of bifidobacteria fermentation is judged by the lactic acid acidity of the crushed material. That is, fermentation is terminated when the lactic acid acidity of the crushed material is in the range of 0.35 to 0.85% by weight, preferably 0.35 to 0.65% by weight. Moreover, in order to completely stop the progress of fermentation, it is preferable to perform heat treatment or freezing treatment as soon as possible after completion of fermentation. Of these, it is particularly preferable to perform a freezing treatment.

このほか、ビフィズス菌以外の細菌の増殖を抑制する目的で、適宜少量の緑茶カテキンに代表される植物抽出物等を添加することができる。これらの添加量は、たとえば緑茶カテキンでは0.01〜0.2重量%である。また、ビフィズス菌の生育を助けるためにブドウ糖などの糖質を添加することもできる。糖質の添加量は、ニンジン破砕物に対し0.1〜10重量%である。さらにビフィズス菌醗酵時のpH制御を目的として、pH調整剤の添加も可能である。ビフィズス菌醗酵に適したpH調整剤としては、酢酸ナトリウム、リン酸三カリウム、リン酸水素二カリウム、リン酸二水素カリウムが挙げられるが、pH緩衝作用を有するものであればこれらに限定されるものではない。pH調整剤の添加量は、ニンジン破砕物に対し0.01〜1重量%である。   In addition, for the purpose of suppressing the growth of bacteria other than Bifidobacterium, a small amount of a plant extract typified by green tea catechin can be added. These addition amounts are, for example, 0.01 to 0.2% by weight for green tea catechins. In addition, a sugar such as glucose can be added to assist the growth of bifidobacteria. The amount of carbohydrate added is 0.1 to 10% by weight with respect to the carrot crushed material. Furthermore, a pH adjuster can be added for the purpose of pH control during fermentation of bifidobacteria. Examples of pH adjusters suitable for Bifidobacteria fermentation include sodium acetate, tripotassium phosphate, dipotassium hydrogen phosphate, and potassium dihydrogen phosphate, but are limited to these as long as they have a pH buffering action. It is not a thing. The addition amount of the pH adjuster is 0.01 to 1% by weight with respect to the carrot crushed material.

本発明で用いるニンジン搾汁とは、ニンジンを必要に応じて破砕などの処理を行った後に、圧搾機や連続遠心分離機などを用いて固形分の一部またはすべてを除去し液状にしたものをいう。加えて、減圧濃縮などの濃縮処理を行ったものも搾汁として用いることができる。   The carrot juice used in the present invention is a product obtained by removing a part or all of the solids using a press or a continuous centrifuge after pulverizing the carrot as necessary. Say. In addition, those that have been subjected to a concentration treatment such as vacuum concentration can also be used as juice.

本発明の野菜飲料組成物は、ビフィズス菌醗酵したニンジン破砕物(A)及びニンジン搾汁(B)を含有させることで得ることができる。(A)と(B)を配合する比率は、重量比で(A):(B)=1:1〜1:10が好ましく、1:2〜1:7がより好ましい。さらには、ニンジン破砕物(C)を含有する野菜飲料組成物が好ましい。(C)の配合する比率は、重量比で(A):(C)=1:3〜9:1がより好ましく、1:2〜5:1がもっとも好ましい。   The vegetable beverage composition of the present invention can be obtained by containing a carrot crushed fermented bifidobacteria (A) and carrot juice (B). The ratio of blending (A) and (B) is preferably (A) :( B) = 1: 1 to 1:10, more preferably 1: 2 to 1: 7 in terms of weight ratio. Furthermore, the vegetable drink composition containing a carrot crushed material (C) is preferable. The ratio of (C) to be blended is more preferably (A) :( C) = 1: 3 to 9: 1, and most preferably 1: 2 to 5: 1 by weight.

本発明の野菜飲料組成物は、さらに柑橘類の果汁を配合することが好ましい。柑橘類とは、ミカン科ミカン亜科ミカン属を指す。具体的には、ユズ、ダイダイ、ブンタン、ポンカン、カボス、スダチ、グレープフルーツ、レモン、ライム、ウンシュウミカン、キシュウミカン、ナツミカン、ハッサク、イヨカン、ヒューガナツ、デコポン、バレンシアオレンジ、ネーブルオレンジ、マンダリンオレンジ、シークァーサー、スウィーティーなどが挙げられる。このうち、ユズ、ダイダイ、グレープフルーツ、レモン、ライム、ウンシュウミカン、キシュウミカン、ナツミカン、ハッサク、バレンシアオレンジ、ネーブルオレンジ、マンダリンオレンジが好ましく、レモン、ライム、ウンシュウミカン、バレンシアオレンジ、グレープフルーツがもっとも好ましい。柑橘類の果汁の配合量は、配合後の野菜飲料組成物の糖酸比が17〜27となる範囲で設定することが好ましく、より好ましくは19〜25である。この糖酸比の範囲に調整するとニンジン特有の風味をさらに改善することができるだけでなく、野菜飲料組成物の全体の風味のバランスを改善することができる。ここにおいて、糖酸比とは屈折率よりショ糖濃度に換算された糖度(ブリックス度ともいう。)と、中和滴定法で測定された酸度の比率をいい、「糖度÷酸度」で表される。具体的には、糖度は、20℃の液体の屈折率を光学的に測定し、ショ糖の質量%に換算した目盛で読み取る専用の糖度計を使って測定し、酸度は、0.1N水酸化ナトリウム溶液による中和的定法(終点はpHガラス電極法を用いた測定でpH8.0)により測定できる。なお酸度は、柑橘類の主要構成有機酸であるクエン酸の重量%に換算した値を用いる。   The vegetable beverage composition of the present invention preferably further contains citrus fruit juice. Citrus refers to the genus Citrus subfamily Mikan. Specifically, Yuzu, Daidai, Bungtan, Ponkan, Kabosu, Sudachi, Grapefruit, Lemon, Lime, Unshu Mikan, Kishu Mikan, Natsumikan, Hassaku, Iyokan, Hugh Natsu, Dekopon, Valencia Orange, Navel Orange, Mandarin Orange, Seaquarser, Sweety etc. are mentioned. Of these, Yuzu, Daidai, Grapefruit, lemon, lime, Satsumikan, Katsumikan, Natsumi, Hassaku, Valencia orange, Navel orange, and Mandarin orange are preferred, and lemon, lime, Satsuma mandarin, Valencia orange, and grapefruit are most preferred. It is preferable to set the compounding quantity of citrus fruit juice in the range from which the sugar-acid ratio of the vegetable drink composition after a mixing | blending will be 17-27, More preferably, it is 19-25. Adjusting to this sugar acid ratio range not only can further improve the carrot-specific flavor, but can also improve the overall flavor balance of the vegetable beverage composition. Here, the sugar acid ratio is the ratio of the sugar content (also referred to as the Brix degree) converted to the sucrose concentration from the refractive index and the acidity measured by the neutralization titration method, and is expressed as “sugar content ÷ acidity”. The Specifically, the sugar content is measured by using a special sugar meter that optically measures the refractive index of a liquid at 20 ° C. and reads it on a scale converted to mass% of sucrose, and the acidity is 0.1 N water. It can be measured by a neutral neutralization method using a sodium oxide solution (the end point is pH 8.0 by measurement using a pH glass electrode method). In addition, the acidity uses the value converted into the weight% of citric acid which is the main constituent organic acid of citrus fruits.

本発明の野菜飲料組成物には、前記成分のほか本発明の効果を損なわない範囲内で通常の食品に一般に用いられる各種成分を必要に応じて適宜配合することができる。配合できる成分としては例えば、りんご果汁など柑橘類以外の果汁、果実ピューレ、果実さのう;ニンジン以外の野菜の搾汁、エキス、破砕物、ペースト;甘味料(ショ糖、ぶどう糖、果糖、乳糖、スクラロース、アスパルテーム、アセスルファムカリウム、ステビア抽出物など);糖アルコール類(還元麦芽糖水飴、マルチトール、キシリトール、ソルビトール、エリスリトールなど);オリゴ糖類(ラクチュロース、フラクトオリゴ糖、ガラクトオリゴ糖、イソマルトオリゴ糖、ラフィノース、パラチノースオリゴ糖、キシロオリゴ糖など);酸味料(クエン酸、リンゴ酸、グルコン酸、酒石酸など);強化剤(L−アスコルビン酸、チアミン塩酸塩、リボフラビン、ピリドキシン塩酸塩、カロテン、α−トコフェロール、コレカルシフェロール、乳酸カルシウム、グルコン酸カルシウム、炭酸カルシウム、水酸化カルシウム、酸化マグネシウム、水酸化マグネシウム、塩化マグネシウム、グルコン酸亜鉛、クエン酸第一鉄アンモニウム、ピロリン酸第二鉄など);増粘多糖類(ペクチン、カラギーナン、グアガム、アラビアガム、ローカストビーンガム、アルギン酸塩、キサンタンガム、大豆多糖類、ジェランガムなど);その他の成分(色素、酸化防止剤、水溶性食物繊維、不溶性食物繊維、動植物エキス、アミノ酸、たんぱく質加水分解ペプチド、トレハロース、シクロデキストリン、キトサン、ヒアルロン酸、フィチン酸、酵母エキス、香料)などが挙げられる。
野菜飲料組成物は、不溶性固形分を3%以上含有する組成物が好ましく、不溶性固形分を5%以上含有する組成物が最も好ましい。
In the vegetable beverage composition of the present invention, in addition to the above-described components, various components generally used in ordinary foods can be appropriately blended as necessary within the range not impairing the effects of the present invention. Ingredients that can be blended include, for example, fruit juices other than citrus fruits such as apple juice, fruit puree, fruit saury; juices, extracts, crushed products, pastes of vegetables other than carrots; sweeteners (sucrose, glucose, fructose, lactose, Sugar alcohols (reduced maltose starch syrup, maltitol, xylitol, sorbitol, erythritol, etc.); oligosaccharides (lacturose, fructooligosaccharides, galactooligosaccharides, isomaltoligosaccharides, raffinose, palatinose) Oligosaccharides, xylo-oligosaccharides, etc.); acidulants (citric acid, malic acid, gluconic acid, tartaric acid, etc.); fortifying agents (L-ascorbic acid, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, carotene, α-tocopherol, cholecalci Ferro , Calcium lactate, calcium gluconate, calcium carbonate, calcium hydroxide, magnesium oxide, magnesium hydroxide, magnesium chloride, zinc gluconate, ferrous ammonium citrate, ferric pyrophosphate, etc.); thickening polysaccharide (Pectin, carrageenan, guar gum, gum arabic, locust bean gum, alginate, xanthan gum, soy polysaccharide, gellan gum, etc.); other ingredients (pigments, antioxidants, water-soluble dietary fiber, insoluble dietary fiber, animal and plant extracts, amino acids , Protein hydrolyzed peptides, trehalose, cyclodextrin, chitosan, hyaluronic acid, phytic acid, yeast extract, fragrance) and the like.
The vegetable beverage composition is preferably a composition containing 3% or more of insoluble solids, and most preferably a composition containing 5% or more of insoluble solids.

以下、本発明の実施例を示すが、本発明はこれらに限定されるものではない。   Examples of the present invention will be described below, but the present invention is not limited thereto.

下記の方法に従って、ニンジン破砕物のビフィズス菌醗酵物を調製し、試験に用いた。   According to the following method, the fermented bifidobacteria of carrot crushed material was prepared and used for the test.

醗酵例1 ビフィズス菌醗酵したニンジン破砕物A(試験例では「醗酵ニンジン破砕物A」と記載する)
ニンジンの皮とヘタを取り除き、約5cm幅に輪切りにして、98℃以上の熱水中に20分間浸漬して加熱処理を行い、コミトロールを使ってピューレ状に破砕し、破砕物の温度を35℃〜40℃まで冷却した。接種するビフィズス菌は、ビフィドバクテリウム・ロンガムBB536株(森永乳業(株)社製)を用いた。スターターは、ビフィドバクテリウム・ロンガムBB536株の菌末を10%濃度になるように滅菌水に分散した菌液を用いた。ラミネート樹脂製のパウチに前記ニンジン破砕物1kgとスターター25gを移し均一にした後にできるだけヘッドスペースが残らないように熱溶着により密封した。密封後、パウチを37℃±1℃の恒温器内に移し2時間保持した後、25℃±1℃に制御した別の恒温器内に移して24時間醗酵処理し、その後冷凍庫に移して凍結させた。醗酵処理終了後のニンジン破砕物の不溶性固形分は76重量%、乳酸酸度は0.38重量%(0.1N水酸化ナトリウム滴定法を使用し乳酸の重量%に換算)、pHは4.3であった。(ガラス電極pHメーターを使用。有姿測定)
Fermentation Example 1 Carrot crushed A fermented with bifidobacteria (In the test example, it is described as “fermented carrot crushed A”)
Remove carrot skin and stickers, cut into 5 cm widths, immerse in hot water at 98 ° C or higher for 20 minutes, heat-treat, crush into puree using commitolol, and adjust the temperature of the crushed material. Cooled to 35-40 ° C. As the Bifidobacterium to be inoculated, Bifidobacterium longum BB536 strain (manufactured by Morinaga Milk Industry Co., Ltd.) was used. As the starter, a bacterial solution in which the bacterial powder of Bifidobacterium longum BB536 strain was dispersed in sterilized water to a concentration of 10% was used. After 1 kg of the carrot crushed material and 25 g of the starter were transferred to a laminate resin pouch and made uniform, they were sealed by heat welding so as to leave as little head space as possible. After sealing, the pouch is transferred to a 37 ° C ± 1 ° C incubator and held for 2 hours, then transferred to another incubator controlled at 25 ° C ± 1 ° C and fermented for 24 hours, then transferred to a freezer and frozen. I let you. The insoluble solid content of the carrot crushed material after the fermentation treatment is 76% by weight, the lactic acid acidity is 0.38% by weight (converted to 0.1% by weight of lactic acid using 0.1N sodium hydroxide titration method), and the pH is 4.3. Met. (Uses glass electrode pH meter. Solid measurement)

醗酵例2 ビフィズス菌醗酵したニンジン破砕物B(試験例では「醗酵ニンジン破砕物B」と記載する)
醗酵例1と同様にして調製したニンジン破砕物およびビフィズス菌スターターを、実施例1と同じ条件でラミネート樹脂製のパウチに充填し、密封した。密封後、パウチを37℃±1℃の恒温器内に移して7時間保持して醗酵処理し、その後冷凍庫に移して凍結させ醗酵を停止させた。醗酵処理終了後のニンジン破砕物の不溶性固形分は76重量%、乳酸酸度は0.37重量%(0.1N水酸化ナトリウム滴定法を使用し乳酸の重量%に換算)、pHは4.3であった。(ガラス電極pHメーターを使用。有姿測定)
Fermentation example 2 Carrot crushed B fermented with bifidobacteria (In the test example, it is described as "fermented carrot crushed B")
The carrot crushed material and bifidobacteria starter prepared in the same manner as in Fermentation Example 1 were filled in a pouch made of laminate resin under the same conditions as in Example 1 and sealed. After sealing, the pouch was transferred into a 37 ° C. ± 1 ° C. incubator and held for 7 hours for fermentation treatment, then transferred to a freezer and frozen to stop the fermentation. The insoluble solid content of the carrot crushed material after the fermentation treatment is 76% by weight, the lactic acid acidity is 0.37% by weight (converted to 0.1% by weight of lactic acid using 0.1N sodium hydroxide titration method), and the pH is 4.3. Met. (Uses glass electrode pH meter. Solid measurement)

試験例1
醗酵ニンジン破砕物A、醗酵ニンジン破砕物B、ニンジン搾汁(濃縮処理なし)、ニンジン破砕物を用いて、表1に示す組成の5種類のニンジン飲料(実施例1〜4、比較例1)を調製した。調製方法は、原材料を混合後、97℃以上で5分間保持することで殺菌した後、密閉できるガラス瓶へ充填し、流水中で冷却することにより行った。調製された野菜飲料組成物の糖酸比は23.0〜24.2であった。なお、表中の数字は重量%を示す。
調製した5つの野菜飲料組成物について、一般の消費者パネル10名に対する飲用評価を実施した。パネルには上記5つの野菜飲料組成物をランダムに飲用し、美味しいと感じた順番に5点から1点の点数をつけることで実施した。10人の点数の平均値を飲用評価スコアとした。評価結果を表1に示す。
Test example 1
Fermented carrot crushed A, fermented carrot crushed B, carrot juice (without concentration treatment), carrot crushed, five types of carrot beverages of the composition shown in Table 1 (Examples 1-4, Comparative Example 1) Was prepared. The preparation method was performed by mixing the raw materials, sterilizing them by holding them at 97 ° C. or higher for 5 minutes, filling them into a glass bottle that can be sealed, and cooling them in running water. The sugar acid ratio of the prepared vegetable beverage composition was 23.0 to 24.2. In addition, the number in a table | surface shows weight%.
About five prepared vegetable drink compositions, drinking evaluation with respect to 10 general consumer panels was implemented. The above five vegetable beverage compositions were taken at random on the panel and given a score of 5 to 1 in the order they felt delicious. The average score of 10 people was taken as a drinking evaluation score. The evaluation results are shown in Table 1.

(表1)

Figure 0005079654
(Table 1)
Figure 0005079654

表1に示したとおり、ビフィズス菌醗酵したニンジン破砕物とニンジン搾汁を含有する野菜飲料組成物は嗜好性が高いことがわかった。また、ビフィズス菌醗酵したニンジン破砕物とニンジン搾汁にニンジン破砕物を含有させた野菜飲料組成物は、含有しない野菜飲料組成物と比較してより高い嗜好性が得られた。さらに、ビフィズス菌醗酵時のニンジン破砕物の温度を低く設定して得られた醗酵ニンジン破砕物Aを含有する野菜飲料組成物は、醗酵ニンジン破砕物Bを含有する野菜飲料組成物より高い嗜好性が得られた。   As shown in Table 1, the vegetable beverage composition containing the carrot crushed fermented bifidobacteria and carrot juice was found to have high palatability. In addition, the vegetable beverage composition in which the carrot crushed fermented bifidobacteria and the carrot juice contained the carrot crushed product had higher palatability compared to the vegetable beverage composition not containing it. Furthermore, the vegetable drink composition containing the fermented carrot crushed A obtained by setting the temperature of the carrot crushed at the time of bifidobacteria fermentation is higher than the vegetable drink composition containing the fermented carrot crushed B. was gotten.

試験例2
醗酵ニンジン破砕物A、ニンジン搾汁(濃縮処理あり)、ニンジン破砕物を用いて表2に示す組成の実施例5〜8および比較例2の5種類の野菜飲料組成物を調製した。調製方法は、各成分を混合し均一にした後、97℃以上で5分間保持することで殺菌した。その後、密閉できるガラス瓶へ充填し、流水中で冷却する方法を用いた。調製された野菜飲料組成物の糖酸比は22.2〜22.8であった。なお表中の数字は重量%を示す。
調製した5つの野菜飲料組成物について、一般の消費者パネル10名に対する飲用評価を実施した。パネルには上記5つの野菜飲料組成物をランダムに飲用し、美味しいと感じた順番に5点から1点の点数をつけることで実施した。10人の点数の平均値を飲用評価スコアとした。評価結果を表1に示す。
Test example 2
Five types of vegetable beverage compositions of Examples 5 to 8 and Comparative Example 2 having the compositions shown in Table 2 were prepared using fermented carrot crushed A, carrot juice (concentrated), and carrot crushed. In the preparation method, each component was mixed and homogenized, and then sterilized by maintaining at 97 ° C. or higher for 5 minutes. Then, the method of filling in the glass bottle which can be sealed and cooling in running water was used. The sugar acid ratio of the prepared vegetable beverage composition was 22.2 to 22.8. In addition, the number in a table | surface shows weight%.
About five prepared vegetable drink compositions, drinking evaluation with respect to 10 general consumer panels was implemented. The above five vegetable beverage compositions were taken at random on the panel and given a score of 5 to 1 in the order they felt delicious. The average score of 10 people was taken as a drinking evaluation score. The evaluation results are shown in Table 1.

(表2)

Figure 0005079654
(Table 2)
Figure 0005079654

表2に示したとおり、ビフィズス菌醗酵をしたニンジン破砕物のみを含有する野菜飲料組成物よりも、ニンジン搾汁を含有する野菜飲料組成物のほうが嗜好性が高く、、ビフィズス菌醗酵をしたニンジン破砕物、ニンジン搾汁およびニンジン破砕物を含有する野菜飲料組成物を含有する野菜飲料組成物のほうが嗜好性が高かった。   As shown in Table 2, the vegetable beverage composition containing carrot juice has higher palatability than the vegetable beverage composition containing only the carrot crushed fermented bifidobacteria, and the carrot fermented with bifidobacteria The vegetable beverage composition containing the vegetable beverage composition containing the crushed material, the carrot juice and the carrot crushed material was higher in palatability.

Claims (4)

ニンジン破砕物のビフィズス菌醗酵物およびニンジン搾汁を配合することを特徴とする野菜飲料組成物。   A vegetable beverage composition comprising a carrot crushed bifidobacteria fermentation product and carrot juice. さらに、ニンジン破砕物を配合することを特徴とする請求項1に記載の野菜飲料組成物。   Furthermore, the vegetable drink composition of Claim 1 which mix | blends a carrot crushed material. ビフィズス菌が、ビフィドバクテリウム・ロンガムであることを特徴とする請求項1または請求項2の何れかに記載の野菜飲料組成物。   The vegetable beverage composition according to claim 1 or 2, wherein the Bifidobacterium is Bifidobacterium longum. さらに、糖酸比が17〜27となるように柑橘類の果汁を配合させることを特徴とする請求項1〜3の何れかに記載の野菜飲料組成物。   Furthermore, citrus fruit juice is mix | blended so that sugar-acid ratio may be 17-27, The vegetable drink composition in any one of Claims 1-3 characterized by the above-mentioned.
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