JP2008131896A - 微生物検出培地 - Google Patents
微生物検出培地 Download PDFInfo
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- JP2008131896A JP2008131896A JP2006320597A JP2006320597A JP2008131896A JP 2008131896 A JP2008131896 A JP 2008131896A JP 2006320597 A JP2006320597 A JP 2006320597A JP 2006320597 A JP2006320597 A JP 2006320597A JP 2008131896 A JP2008131896 A JP 2008131896A
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- 244000005700 microbiome Species 0.000 title claims abstract description 23
- 239000001963 growth medium Substances 0.000 title claims abstract description 13
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 21
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 21
- 108010031396 Catechol oxidase Proteins 0.000 claims abstract description 16
- 102000030523 Catechol oxidase Human genes 0.000 claims abstract description 16
- 239000002609 medium Substances 0.000 claims description 23
- 238000001514 detection method Methods 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 4
- 235000005487 catechin Nutrition 0.000 description 12
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- 229950001002 cianidanol Drugs 0.000 description 11
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- 235000009569 green tea Nutrition 0.000 description 8
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- 238000002360 preparation method Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
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- 238000002835 absorbance Methods 0.000 description 2
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- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 description 1
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
- 241000589596 Thermus Species 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- PLKYGPRDCKGEJH-UHFFFAOYSA-N azane;2-hydroxypropane-1,2,3-tricarboxylic acid;iron Chemical compound N.[Fe].OC(=O)CC(O)(C(O)=O)CC(O)=O PLKYGPRDCKGEJH-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 150000001765 catechin Chemical class 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 229960003964 deoxycholic acid Drugs 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 150000007946 flavonol Chemical class 0.000 description 1
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
- 235000011957 flavonols Nutrition 0.000 description 1
- -1 for example Proteins 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
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- 150000007524 organic acids Chemical class 0.000 description 1
- 239000007793 ph indicator Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- NRHMKIHPTBHXPF-TUJRSCDTSA-M sodium cholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 NRHMKIHPTBHXPF-TUJRSCDTSA-M 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- FHHPUSMSKHSNKW-SMOYURAASA-M sodium deoxycholate Chemical compound [Na+].C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 FHHPUSMSKHSNKW-SMOYURAASA-M 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
【解決手段】ポリフェノールオキシダーゼを含有することを特徴とする、ポリフェノール類含有試料用微生物検出培地。
【選択図】なし
Description
近年、カテキン等のポリフェノール類が体脂肪の増加抑制効果や血中コレステロール低下効果等の生理活性を有することが明らかになり、緑茶飲料やカテキン含有食品等が注目され、広く使用されている。
Kansenshogaku Zasshi. 1994 Dec;68(12):1518-22. Nippon Saikingaku Zasshi. 1991 Sep;46(5):839-45. Japanese
従って、本発明の目的は、ポリフェノール類を含有する試料であっても、微生物を精度良く検出できる手段を提供することにある。
また本発明は、ポリフェノールオキシダーゼを含有する培地に、ポリフェノール類含有試料を接種して培養することを特徴とするポリフェノール類含有試料からの微生物の検出方法を提供するものである。
(1)培地の作製
標準寒天培地(SPC)(ペプトン5g、酵母エキス2.5g、ブドウ糖1g、寒天15g:日水製薬社製)1リットル使用量を1リットルの精製水に加え、121℃、15分間、高圧蒸気滅菌する。これを2本作製した。滅菌後培地を50℃に保温し、一方のSPCにはろ過滅菌したラッカーゼ(ラッカーゼダイワY120)を108,000POU/Lになるように加え酵素加標準寒天培地(ESPC)とした。
但し、1POUは4−アミノアンチピリンとフェノールにpH4.5、30℃で作用するとき、ラッカーゼが触媒する酸化縮合反応により生成するキノンイミン色素の505nmにおける吸光度を反応初期1分間に0.1増加させるのに必要な酵素量とする。
カテキン含有緑茶飲料を無菌的に9mLずつ、滅菌試験管に分注した。対照として、9mLの滅菌生理食塩水を準備した。
供試菌株はトリプトソイブイヨンで24時間前培養したものを用い、これを滅菌生理食塩水及びカテキン含有緑茶飲料で10-1〜10-8まで希釈した。希釈したそれぞれの菌液を滅菌空シャーレに1mLずつ接種し、予め滅菌保温したSPC及びESPCそれぞれにて混釈培養を行った。35℃、48時間培養後、発育したコロニー数を計測した。
(1)培地の作製
標準寒天培地(SPC)(ペプトン5g、酵母エキス2.5g、ブドウ糖1g、寒天15g:日水製薬社製)1リットル使用量を1リットルの精製水に加え、121℃、15分間、高圧蒸気滅菌する。滅菌後培地を50℃に保温し、これにろ過滅菌したラッカーゼを108,000POU/L(1g/L)、10,800POU/L(0.1g/L)、1,080POU/L(0.01g/L)、0 POU/L(0g/L)になるようにそれぞれ加え使用した。
(2)希釈液の作製
カテキン含有緑茶飲料を無菌的に9mLずつ、滅菌試験管に分注した。対照として、9mLの滅菌生理食塩水を準備した。
(3)菌株の供試
供試菌株はトリプトソイブイヨンで24時間前培養したものを用い、これを滅菌生理食塩水及びカテキン含有緑茶飲料で10-1〜10-8まで希釈した。希釈したそれぞれの菌液を滅菌空シャーレに1mLずつ接種し、予め滅菌保温したそれぞれの培地にて混釈培養を行った。35℃、48時間培養後、発育したコロニー数を計測した。
結果を表2に示す。
Claims (4)
- ポリフェノールオキシダーゼを含有することを特徴とする、ポリフェノール類含有試料用微生物検出培地。
- ポリフェノールオキシダーゼ含有量が使用時の濃度として50,000POU/L以上である請求項1記載の培地。
- ポリフェノールオキシダーゼを含有する培地に、ポリフェノール類含有試料を接種して培養することを特徴とするポリフェノール類含有試料からの微生物の検出方法。
- 培地中のポリフェノールオキシダーゼ含有量が使用時の濃度として50,000POU/L以上である請求項3記載の検出方法。
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013516174A (ja) * | 2009-12-30 | 2013-05-13 | スリーエム イノベイティブ プロパティズ カンパニー | グルコースオキシダーゼを産生するカビの迅速検出 |
WO2017154933A1 (ja) * | 2016-03-08 | 2017-09-14 | 大日本印刷株式会社 | 内容物充填システムにおける初発菌確認方法、内容物充填システムの検証方法および培地 |
JP2017159928A (ja) * | 2016-03-08 | 2017-09-14 | 大日本印刷株式会社 | 内容物充填システムの検証方法および培地 |
JP2017186089A (ja) * | 2017-06-29 | 2017-10-12 | 大日本印刷株式会社 | 内容物充填システムにおける初発菌確認方法 |
JP2017186070A (ja) * | 2016-04-08 | 2017-10-12 | 大日本印刷株式会社 | 内容物充填システムにおける初発菌確認方法 |
JP2017186090A (ja) * | 2017-06-29 | 2017-10-12 | 大日本印刷株式会社 | 内容物充填システムにおける初発菌確認方法 |
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JPH0549401A (ja) * | 1991-08-21 | 1993-03-02 | Shokuhin Sangyo Chokoatsu Riyou Gijutsu Kenkyu Kumiai | 茶飲料の製造方法 |
JPH09132532A (ja) * | 1995-09-06 | 1997-05-20 | Mitsui Norin Kk | 抗生物質の抗菌力増強方法 |
JP2006158252A (ja) * | 2004-12-06 | 2006-06-22 | National Institute Of Advanced Industrial & Technology | 耐熱性ラッカーゼおよびその製造法 |
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2006
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH0549401A (ja) * | 1991-08-21 | 1993-03-02 | Shokuhin Sangyo Chokoatsu Riyou Gijutsu Kenkyu Kumiai | 茶飲料の製造方法 |
JPH09132532A (ja) * | 1995-09-06 | 1997-05-20 | Mitsui Norin Kk | 抗生物質の抗菌力増強方法 |
JP2006158252A (ja) * | 2004-12-06 | 2006-06-22 | National Institute Of Advanced Industrial & Technology | 耐熱性ラッカーゼおよびその製造法 |
Cited By (7)
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WO2017154933A1 (ja) * | 2016-03-08 | 2017-09-14 | 大日本印刷株式会社 | 内容物充填システムにおける初発菌確認方法、内容物充填システムの検証方法および培地 |
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US10875756B2 (en) | 2016-03-08 | 2020-12-29 | Dai Nippon Printing Co., Ltd. | Initial bacteria confirmation method in content filling system, method for verifying content filling system, and culture medium |
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