JP2008127304A - Agent for enhancing dental quality, oral composition containing the same, food and drink and method for producing them - Google Patents
Agent for enhancing dental quality, oral composition containing the same, food and drink and method for producing them Download PDFInfo
- Publication number
- JP2008127304A JP2008127304A JP2006312310A JP2006312310A JP2008127304A JP 2008127304 A JP2008127304 A JP 2008127304A JP 2006312310 A JP2006312310 A JP 2006312310A JP 2006312310 A JP2006312310 A JP 2006312310A JP 2008127304 A JP2008127304 A JP 2008127304A
- Authority
- JP
- Japan
- Prior art keywords
- acid
- seaweed
- tooth
- fossilized
- xylitol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- GYQBBRRVRKFJRG-UHFFFAOYSA-L disodium pyrophosphate Chemical compound [Na+].[Na+].OP([O-])(=O)OP(O)([O-])=O GYQBBRRVRKFJRG-UHFFFAOYSA-L 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 229940048879 dl tartaric acid Drugs 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 229940125532 enzyme inhibitor Drugs 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013861 fat-free Nutrition 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 235000013332 fish product Nutrition 0.000 description 1
- 229940091249 fluoride supplement Drugs 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 229950006191 gluconic acid Drugs 0.000 description 1
- 235000012209 glucono delta-lactone Nutrition 0.000 description 1
- 239000000182 glucono-delta-lactone Substances 0.000 description 1
- 229960003681 gluconolactone Drugs 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000007937 lozenge Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 description 1
- 239000001095 magnesium carbonate Substances 0.000 description 1
- 229910000021 magnesium carbonate Inorganic materials 0.000 description 1
- 125000005341 metaphosphate group Chemical group 0.000 description 1
- LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 235000011962 puddings Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 208000008655 root caries Diseases 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 235000012046 side dish Nutrition 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 229940100890 silver compound Drugs 0.000 description 1
- 150000003379 silver compounds Chemical class 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 235000011888 snacks Nutrition 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000015424 sodium Nutrition 0.000 description 1
- HELHAJAZNSDZJO-OLXYHTOASA-L sodium L-tartrate Chemical compound [Na+].[Na+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O HELHAJAZNSDZJO-OLXYHTOASA-L 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 239000001394 sodium malate Substances 0.000 description 1
- 229960004711 sodium monofluorophosphate Drugs 0.000 description 1
- 239000001433 sodium tartrate Substances 0.000 description 1
- 229960002167 sodium tartrate Drugs 0.000 description 1
- KZQSXALQTHVPDQ-UHFFFAOYSA-M sodium;butanedioate;hydron Chemical compound [Na+].OC(=O)CCC([O-])=O KZQSXALQTHVPDQ-UHFFFAOYSA-M 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 229910001631 strontium chloride Inorganic materials 0.000 description 1
- AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical compound [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- VXYADVIJALMOEQ-UHFFFAOYSA-K tris(lactato)aluminium Chemical compound CC(O)C(=O)O[Al](OC(=O)C(C)O)OC(=O)C(C)O VXYADVIJALMOEQ-UHFFFAOYSA-K 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 235000019263 trisodium citrate Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 235000008939 whole milk Nutrition 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
- 235000005074 zinc chloride Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9717—Rhodophycota or Rhodophyta [red algae], e.g. Porphyra
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/04—Rhodophycota or rhodophyta (red algae), e.g. Porphyra
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0216—Solid or semisolid forms
- A61K8/022—Powders; Compacted Powders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
Abstract
Description
本発明は、歯質強化剤及びこれを含有する口腔用組成物並びに飲食物とそれらの製造方法に関する。 The present invention relates to a tooth strengthening agent, an oral composition containing the same, food and drink, and methods for producing them.
一般に、齲蝕は、ストレプトコッカス・ミュータンス（Ｓｔｒｅｐｔｏｃｏｃｃｕｓ ｍｕｔａｎｓ）やストレプトコッカス・ソブリナス（Ｓｔｒｅｐｔｏｃｏｃｃｕｓ ｓｏｂｒｉｎｕｓ）等の口腔内レンサ球菌（虫歯菌）が歯牙表面に付着し、これらの細菌が持っているグルコシルトランスフェラーゼ酵素の働きでグルカンを産生し、プラーク（歯垢）を形成することから始まる。そのプラーク中で、上記細菌が食物残渣中の砂糖やデンプン等を代謝することにより生成される酸が、歯牙エナメル質を脱灰し、いわゆる初期の齲蝕状態とする。 In general, caries is caused by the action of glucosyltransferase enzyme in the oral streptococcus (caries fungus) such as Streptococcus mutans and Streptococcus sobrinus, which adhere to the tooth surface. It begins with producing glucan and forming plaques. In the plaque, the acid produced by the bacteria metabolizing sugar, starch, etc. in the food residue decalcifies the tooth enamel, so that it becomes a so-called initial caries state.
唾液にはカルシウムとりん酸塩が存在し、これらが、上記脱灰部分を修復すなわち再石灰化することによって、歯を元の状態に戻す作用をしている。つまり、歯牙表面では、脱灰と再石灰化という相反する現象が常に生起し、通常は所要のバランスを保っている。しかし、そのバランスはプラークが増大すると脱灰の方に傾き、齲蝕が進行する。 Calcium and phosphate are present in saliva, and these act to restore the teeth by restoring or demineralizing the demineralized portion. In other words, contradictory phenomena of decalcification and remineralization always occur on the tooth surface, and the required balance is usually maintained. However, the balance tends to decalcify as plaque increases and caries progresses.
歯牙表面のエナメル質を構成する結晶は、六方晶系のハイドロキシアパタイトＣａ１０（ＰＯ４）６ＯＨ２で、りん酸カルシウムから構成されている。初期の齲蝕で認められる脱灰は歯牙エナメル質無機成分の溶解であり、再石灰化は溶け残った既存のりん酸カルシウム結晶の修復と再成長および本来のエナメル質には存在していない結晶の出現であるということができる。齲蝕予防とは、虫歯菌に対する付着阻害、抗菌、あるいはまた虫歯菌のグルカン合成に関わるグルコシルトランスフェラーゼに対する阻害、歯の再石灰化促進、歯の耐酸性向上等をいい、その中でも特に歯の再石灰化促進、耐酸性向上等の効果を付与することを歯質強化という。 The crystal constituting the enamel of the tooth surface is hexagonal hydroxyapatite Ca 10 (PO 4 ) 6 OH 2 and is composed of calcium phosphate. The demineralization observed in the initial caries is dissolution of the tooth enamel inorganic component, and remineralization is the repair and regrowth of the existing calcium phosphate crystals that remain undissolved and the crystals that are not present in the original enamel. It can be said that it is an appearance. Caries prevention refers to inhibition of caries fungus adhesion, antibacterial activity, or inhibition of glucosyltransferase involved in carcinogenic glucan synthesis, promotion of tooth remineralization, improvement of tooth acid resistance, etc. Giving the effect of promoting oxidization and improving acid resistance is called enhancement of tooth structure.
また、象牙質の知覚過敏症は酸等が露出した象牙質から石灰分を溶出せしめることで象牙細管が開口し、そこに入り込んだ液体が熱や物理的な力などを受け、歯随の神経を刺激し痛みを感じるものである。従って、治療及び予防のためには象牙細管を閉塞もしくは象牙質の耐酸性を向上させる必要がある。根面う蝕の予防および治療においても、セメント質ならびに象牙質の耐酸性を向上させる必要がある。 Dentine hypersensitivity is caused by leaching of lime from acid-exposed dentin to open dentinal tubules, and the liquid that enters them receives heat, physical force, etc. It stimulates and feels pain. Therefore, for treatment and prevention, it is necessary to block the dentinal tubule or improve the acid resistance of the dentin. In the prevention and treatment of root caries, it is necessary to improve the acid resistance of cementum and dentin.
従来、齲蝕予防のために、虫歯菌に対する歯牙付着阻害剤、抗菌剤、あるいはまた、虫歯菌のグルカン形成を抑制するグルコシルトランスフェラーゼ酵素阻害剤等が開発されている。しかし、たとえば抗菌剤は虫歯菌のみに抗菌作用を示す特異的な素材ではなく、安全性に問題があり、グルコシルトランスフェラーゼ酵素阻害剤は唾液による影響を受けやすいという問題がある。 Conventionally, for preventing dental caries, tooth adhesion inhibitors, antibacterial agents for caries fungi, and glucosyltransferase enzyme inhibitors for suppressing glucan formation of caries fungi have been developed. However, for example, an antibacterial agent is not a specific material that exhibits antibacterial action only against caries bacteria, but has a problem in safety, and a glucosyltransferase enzyme inhibitor has a problem that it is easily affected by saliva.
また、歯牙の無機成分と類似の結晶構造を有するハイドロキシアパタイトとフッ化物を配合し、歯脱灰表層部を再石灰化する虫歯予防組成物が知られているが（例えば、特許文献１参照）、フッ化ナトリウム、モノフルオロリン酸ナトリウムまたはフッ化第一スズ等のフッ化物を口腔用組成物や飲食物に配合することは、安全性の点から問題がある。 Further, a dental caries prevention composition is known in which hydroxyapatite having a crystal structure similar to that of the tooth and a fluoride are blended to remineralize the tooth demineralized surface layer (see, for example, Patent Document 1). Incorporating fluorides such as sodium fluoride, sodium monofluorophosphate or stannous fluoride into oral compositions and foods and drinks is problematic from the viewpoint of safety.
さらに、ハイドロキシアパタイトの微粒子とキシリトールを組み合せ使用することによって、脱灰した歯牙エナメル質を再石灰化することが知られているが（例えば、特許文献２参照）、工業的に製造されたハイドロキシアパタイトは、化学的に安定な化合物で反応性に乏しく、厳密には生体における歯牙を構成するハイドロキシアパタイトとはその結晶構造が異なるため、再石灰化の効果が十分でない。 Furthermore, it is known to remineralize decalcified tooth enamel by using a combination of hydroxyapatite fine particles and xylitol (see, for example, Patent Document 2), but industrially produced hydroxyapatite. Is a chemically stable compound and poor in reactivity, and strictly speaking, its effect on remineralization is not sufficient because it has a crystal structure different from that of hydroxyapatite that constitutes teeth in living bodies.
なお、食品等への使用を考慮すると天然物由来の組成物を齲蝕予防組成物として用いることが適当であると考えられ、この条件に合うものを探してみると例えば茶抽出物がある。しかし、単なる茶抽出物では、従来、抗菌やグルコシルトランスフェラーゼ阻害等の齲蝕予防効果は知られているが、再石灰化促進や耐酸性向上等といった歯質強化効果は全く知られていない。また、茶抽出物からポリフェノール類を除去することにより、歯質強化にとってプラスに働くミネラルの比率を相対的に高めた歯質強化剤が知られているが（例えば、特許文献３参照）、この歯質強化剤は、天然物由来ではあるが、安全性、効果の面で十分とは言えないものである。 In consideration of use in foods and the like, it is considered appropriate to use a composition derived from a natural product as a composition for preventing dental caries. However, mere tea extract is conventionally known to have caries prevention effects such as antibacterial activity and glucosyltransferase inhibition, but has no known dental enhancement effect such as promoting remineralization or improving acid resistance. In addition, a tooth enhancer is known in which a polyphenols is removed from a tea extract to relatively increase the ratio of minerals that work positively for tooth enhancement (see, for example, Patent Document 3). Tooth enhancers are derived from natural products, but are not sufficient in terms of safety and effectiveness.
また、象牙質の知覚過敏症の治療に用いられてきた薬剤としては、フッ化ジアミン銀、硝酸銀、塩化ストロンチウム、フッ化第一スズ、フッ化ナトリウム、フッ化アルミニウム、乳酸アルミニウム、塩化亜鉛、水酸化カルシウム、パラホルムアルデヒド、ホルムアルデヒドなどの化合物がある。これらのうち、銀化合物は、象牙細管の閉塞性には優れるものの、著しい着色性を有し、一般的な使用には適していないという欠点を有している。また、それ以外の化合物についても、未だ充分な効果が得られているとはいえない。 Drugs that have been used to treat dentine hypersensitivity include silver diamine diamine, silver nitrate, strontium chloride, stannous fluoride, sodium fluoride, aluminum fluoride, aluminum lactate, zinc chloride, water There are compounds such as calcium oxide, paraformaldehyde, formaldehyde. Among these, silver compounds have a drawback that they have excellent colorability and are not suitable for general use, although they are excellent in occlusion of dentinal tubules. Moreover, it cannot be said that sufficient effects have been obtained for other compounds.
一方、石化海藻については、免疫調節の不全に起因する体の異常を治療する効果や、固形又は半固形食品において官能特性や物理特性を向上させる効果、整腸効果等が知られている（例えば、特許文献４、５、６参照）。また、石化海藻には、高濃度にフッ素化合物が含まれていることが知られているが（例えば、特許文献７参照）、物理的方法による処理では、フッ素がほとんど作用せず、実際に歯質強化剤として使用することが出来ない。 On the other hand, as for petrified seaweeds, the effects of treating body abnormalities caused by immunoregulation failure, the effects of improving sensory characteristics and physical characteristics in solid or semi-solid foods, the effects of intestinal regulation, etc. are known (for example, Patent Documents 4, 5, and 6). In addition, it is known that petrochemical seaweed contains a high concentration of a fluorine compound (see, for example, Patent Document 7). However, in the treatment by a physical method, the fluorine hardly acts and the tooth is actually used. It cannot be used as a quality enhancer.
上記に鑑み、本発明は、口腔用組成物や飲食物に使用しても安全性において問題がなく、しかも、脱灰した歯牙エナメル質の再石灰化を効果的に促進するとともに、歯の耐酸性を向上することができる歯質強化剤及びこれを含有した口腔用組成物並びに飲食物とそれらの製造方法の提供を目的とするものである。 In view of the above, the present invention has no problem in safety even when used in oral compositions and foods and drinks, and effectively promotes remineralization of demineralized tooth enamel and is resistant to dental acid. It aims at provision of the tooth strengthening agent which can improve a property, the composition for oral cavity containing this, food-drinks, and those manufacturing methods.
本発明者は、鋭意研究を重ねた結果、フッ素化合物を高濃度に含む石化海藻と酸を有効成分とする歯質強化剤が上記目的を達成するとの知見を得て本発明を完成した。 As a result of intensive studies, the present inventor obtained the knowledge that a fossilized seaweed containing a high concentration of a fluorine compound and a tooth strengthening agent containing an acid as an active ingredient have achieved the above object and completed the present invention.
すなわち、本発明の歯質強化剤は、石化海藻と酸を有効成分とするもの、あるいはまた、石化海藻と酸とキシリトールを有効成分とするものである。本発明の歯質強化剤は、石化海藻と酸が入っていれば良く、あらかじめ石化海藻と酸を混合処理して配合しても良い。 That is, the dentin strengthening agent of the present invention contains a petrified seaweed and an acid as active ingredients, or a petrified seaweed, an acid and xylitol as active ingredients. The dentin strengthening agent of the present invention only needs to contain fossilized seaweed and acid, and may be blended by mixing the fossilized seaweed and acid in advance.
また、本発明の歯質強化用の口腔用組成物は、石化海藻と酸を配合するもの、あるいはまた、石化海藻と酸を混合処理したものを配合するもの、あるいはさらにキシリトールを配合するものである。 In addition, the oral cavity composition for dental enhancement according to the present invention is a composition containing a fossilized seaweed and an acid, a composition obtained by mixing a fossilized seaweed and an acid, or a composition containing xylitol. is there.
さらに、本発明の歯質強化用の飲食物は、石化海藻と酸を配合するもの、あるいはまた、石化海藻と酸を混合処理したものを配合するもの、あるいはさらにキシリトールを配合するものである。 Furthermore, the food and drink for strengthening the tooth structure of the present invention is one that contains a petrified seaweed and an acid, one that contains a mixture of a petrified seaweed and an acid, or one that further contains xylitol.
また、本発明の歯質強化剤の製造方法は、石化海藻と酸を含有させる、もしくはあらかじめ石化海藻と酸を混合した処理物を混合するもの、あるいはさらにキシリトールを添加する方法である。 In addition, the method for producing a tooth strengthening agent of the present invention is a method of adding a fossilized seaweed and an acid, or mixing a processed product obtained by mixing a fossilized seaweed and an acid in advance, or further adding xylitol.
さらに、本発明の口腔用組成物の製造方法は、歯質強化剤として石化海藻と酸を各別に配合させる、あるいはまた、歯質強化剤として石化海藻と酸を混合処理したものを配合する、あるいはそれらの歯質強化剤にさらにキシリトールを配合する方法である。 Furthermore, the method for producing an oral composition of the present invention comprises mixing a petrified seaweed and an acid separately as a tooth strengthening agent, or blending a mixture of a petrified seaweed and an acid as a tooth strengthening agent, Or it is the method of mix | blending xylitol further in those tooth strengthening agents.
本発明の石化海藻と酸を有効成分とする歯質強化剤およびその製造方法であれば、従来のフッ素を含有するカルシウム剤に比して歯質の耐酸性促進及び再石化促進という二つの優れた効果を有する歯質強化剤を提供することができ、安全性においても問題がない。 If it is a tooth strengthening agent which uses the petrochemical seaweed and acid of this invention as an active ingredient, and its manufacturing method, compared with the conventional calcium agent containing a fluorine, it is two excellent of the acid resistance acceleration | stimulation of a tooth substance, and a recalcification promotion. Can be provided, and there is no problem in safety.
また、石化海藻と酸、さらにキシリトールを有効成分とする歯質強化剤およびその製造方法であれば、特に優れた歯質強化剤を提供することができる。 Moreover, if it is a tooth strengthening agent which uses a petrified seaweed, an acid, and also xylitol as an active ingredient, and its manufacturing method, a particularly excellent tooth strengthening agent can be provided.
さらに、本発明の歯質強化剤およびその製造方法は、歯牙エナメル質の耐酸性を向上させることのみならず、象牙質の耐酸性をも向上させることができるので、それによって齲蝕を積極的に抑制し、象牙質知覚過敏症をも予防、治療することができる。 Furthermore, the dentin strengthening agent and the method for producing the same according to the present invention can improve not only the acid resistance of the tooth enamel, but also the acid resistance of the dentin, thereby actively preventing caries. It can suppress and prevent and treat dentine hypersensitivity.
本発明で使用する石化海藻とは、海藻が海中のカルシウムやマグネシウム等のミネラル成分を吸着して得られるものであり、好ましくは紅藻類、より好ましくはサンゴモ科海藻の石灰質残渣である。 The fossilized seaweed used in the present invention is obtained by adsorbing seawater minerals such as calcium and magnesium, and is preferably a calcareous residue of red algae, more preferably coralaceae seaweed.
例えば、Ｌｉｔｈｏｔｈａｍｎｉｕｍ ｃｏｒａｌｌｉｏｉｄｅｓ、Ｐｈｙｍａｔｏｌｉｔｈｏｎ ｃａｌｃａｒｅｕｍ及びＬｉｔｈｏｔｈａｍｎｉｕｍ ｇｌａｃｉａｌｅ種等の、サンゴモ科の仲間を含む紅藻類の石灰質残渣が挙げられる。 Examples include calcareous residues of red algae including coral family, such as Lithothamnium coralloides, Phymatolithon calcareum, and Lithothamnium gliale species.
サンゴモの一種であるＬｉｔｈｏｔｈａｍｎｉｕｍ ｃｏｒａｌｌｉｏｉｄｅｓ（Ｌｉｔｈｏｔｈａｍｎｉｕｍ ｃａｌｃａｌｅｕｍ、Ｐｈｙｍａｔｏｌｉｔｈｏｎ ｃａｌｃａｒｅｕｍともいう）は冷たく穏やかな海に非常に豊富にある海藻であり、このサンゴモが枯れた後に残る石灰質残渣は、９０重量％以上が無機質であり、主に炭酸カルシウム及び炭酸マグネシウムからなる。 Lithothamnium coralloides (also called Lithothamium calcareum, also known as Phymatolithon calcareum), which is a kind of coral, is a seaweed that is very abundant in the cold and calm sea, and the calcareous residue remaining after the coral is over 90% by weight , Consisting mainly of calcium carbonate and magnesium carbonate.
上記した石化海藻は、海底より浚渫された状態でも使用できるが、砂、貝殻等が含まれているので、これらの一部又は全部が、海水及び／又は真水での洗浄、篩別、手作業による選別等の方法によって除去されていることが好ましく、更に、過酸化水素水処理や加熱処理等で殺菌し、乾燥したものは、飲食品用途として、細菌等の食品衛生規制に適合するため、より好ましい。なお、アクアミネラル（日本バイオコン株式会社製）、ＡＱＵＡＭＩＮ（ＭＡＲＩＧＯＴ ＬＴＤ．製）等、市販の飲食品用石化海藻粉末も使用できる。 The above petrochemical seaweed can be used even when dredged from the seabed, but because it contains sand, shells, etc., some or all of them are washed with seawater and / or fresh water, sieved, and manual work. It is preferable to be removed by a method such as sorting by, and further, sterilized by drying with hydrogen peroxide solution or heat treatment, etc., and dried, to meet food hygiene regulations such as bacteria for food and drink use, More preferred. Commercially available fossilized seaweed powder for food and drink such as Aqua Mineral (manufactured by Nippon Biocon Co., Ltd.) and AQUAMIN (manufactured by MARIGOT LTD.) Can also be used.
本発明で用いられる酸は、無機の強酸である塩酸や硫酸等、食品等への適用が認可されている酸味料等、酸味を呈するものであればよい。具体的には、アスコルビン酸、ソルビン酸、安息香酸、アジピン酸、クエン酸（結晶）（無水）、クエン酸三ナトリウム、グルコノデルタラクトン、グルコン酸、コハク酸、コハク酸一ナトリウム、コハク酸二ナトリウム、酢酸ナトリウム（結晶）（無水）、ＤＬ−酒石酸、Ｌ−酒石酸、ＤＬ−酒石酸ナトリウム、Ｌ−酒石酸ナトリウム、乳酸、乳酸ナトリウム、二酸化炭素、酢酸、フマル酸、フマル酸一ナトリウム、ＤＬ−リンゴ酸、ＤＬ−リンゴ酸ナトリウム、リン酸、イタコン酸、α−ケトグルタル酸、フィチン酸、ピロリン酸二水素二ナトリウム、酸性メタリン酸ナトリウムの他、食酢や果汁等が挙げられる。これらのものを１種類もしくは２種類以上用いて使用しても良い。 The acid used in the present invention may be any acid that exhibits acidity, such as hydrochloric acid or sulfuric acid, which are strong inorganic acids, and acidulants that are approved for use in foods. Specifically, ascorbic acid, sorbic acid, benzoic acid, adipic acid, citric acid (crystal) (anhydrous), trisodium citrate, glucono delta lactone, gluconic acid, succinic acid, monosodium succinate, succinic acid disodium Sodium, sodium acetate (crystal) (anhydrous), DL-tartaric acid, L-tartaric acid, DL-sodium tartrate, sodium L-tartrate, lactic acid, sodium lactate, carbon dioxide, acetic acid, fumaric acid, monosodium fumarate, DL-apple Examples include acid, DL-sodium malate, phosphoric acid, itaconic acid, α-ketoglutaric acid, phytic acid, disodium dihydrogen pyrophosphate, sodium acid metaphosphate, vinegar, and fruit juice. These may be used alone or in combination of two or more.
本発明の歯質強化剤における石化海藻と酸との割合は、本発明の効果が得られれば適宜選択しそれらの割合を決定することができるが、好ましくは石化海藻１重量部に対して０．１〜１０重量部、更に好ましくは、０．３〜３重量部である。この範囲であれば、石化海藻中のフッ素の溶出性の向上が認められる。 The ratio of the fossilized seaweed and the acid in the dentifrice enhancer of the present invention can be appropriately selected and determined as long as the effect of the present invention is obtained, but is preferably 0 with respect to 1 part by weight of the fossilized seaweed. 0.1 to 10 parts by weight, more preferably 0.3 to 3 parts by weight. If it is this range, the improvement of the elution property of the fluorine in petrified seaweed will be recognized.
上述した本発明の歯質強化剤は、上記した石化海藻と酸を、もしくはあらかじめ石化海藻と酸を混合した溶液、石化海藻と酸を混合した溶液を乾燥させた処理物を適当な液体担体に溶解するか若しくは分散させ、あるいは、適当な粉末担体と混合するか若しくはこれに吸着させ、場合によっては、乳化剤，分散剤，懸濁剤，展着剤，浸透剤，湿潤剤又は安定剤等を添加し、乳剤，水和剤，粉剤又は錠剤等に製剤化して使用に供することも可能である。 The above-described dentifrice enhancer of the present invention uses the above-mentioned fossilized seaweed and acid, or a solution obtained by previously mixing a fossilized seaweed and an acid, and a processed product obtained by drying a solution obtained by mixing a fossilized seaweed and an acid, in a suitable liquid carrier. Dissolve or disperse, or mix with or adsorb to a suitable powder carrier, and optionally add emulsifiers, dispersants, suspending agents, spreading agents, penetrating agents, wetting agents, stabilizers, etc. It is also possible to add it and make it into an emulsion, a wettable powder, a powder or a tablet for use.
さらに、本発明の歯質強化剤は、石化海藻と酸を、もしくはあらかじめ石化海藻と酸を混合した処理物を使用しても、歯の耐酸性と再石灰化を促進することで歯質を十分に強化することができるが、さらにキシリトールを配合することにより、耐酸性と再石灰化を著しく促進させることができる。キシリトールの使用量としては、本発明の効果が得られれば適宜選択しその割合を決定することができるが、好ましくは石化海藻１重量部に対して1〜１００重量部、更に好ましくは、１０〜５０重量部である。 Furthermore, the tooth strengthening agent of the present invention promotes the acid resistance and remineralization of teeth by using a processed product in which fossilized seaweed and acid or a premixed fossilized seaweed and acid is used. Although it can fully strengthen, acid resistance and remineralization can be remarkably promoted by adding xylitol. The amount of xylitol to be used can be appropriately selected and determined as long as the effects of the present invention are obtained, but preferably 1 to 100 parts by weight, more preferably 10 to 10 parts by weight per petrochemical seaweed. 50 parts by weight.
本発明においてあらかじめ石化海藻と酸を混合処理する方法としては、例えば、石化海藻と酸の粉末同士を混合しても、石化海藻と酸の粉末を水等の溶媒に溶解させて混合しても良い。もしくは、例えば、石化海藻を液状の酸や酸水溶液に溶解させて混合しても良い。また、石化海藻と酸を混合した石化海藻溶液を乾燥させた処理物を調製しても良く、石化海藻と酸との混合処理は、いずれの方法で行っても良い。 In the present invention, as a method of mixing the petrified seaweed and the acid in advance, for example, the petrified seaweed and the acid powder may be mixed, or the petrified seaweed and the acid powder may be dissolved in a solvent such as water and mixed. good. Alternatively, for example, petrified seaweed may be dissolved and mixed in a liquid acid or aqueous acid solution. Moreover, the processed material which dried the petrified seaweed solution which mixed the petrified seaweed and an acid may be prepared, and the mixing process of a petrified seaweed and an acid may be performed by any method.
石化海藻と酸を混合することにより、石化海藻中のフッ素の溶出性を向上させ、石化海藻中のミネラル成分も相乗的に作用し、歯の耐酸性と再石灰化を促進することで歯質を強化することができる。 By mixing the fossilized seaweed and acid, the elution of fluorine in the fossilized seaweed is improved, and the mineral components in the fossilized seaweed also act synergistically to promote acid resistance and remineralization of the teeth. Can be strengthened.
さらに、石化海藻と酸を有効成分とする歯質強化剤にキシリトールを併用することにより、歯の脱灰層深層からの再石灰化を促し、耐酸性と再石灰化を著しく促進させることができる。 Furthermore, by using xylitol together with a dentifrice-enhancing agent that contains petrified seaweed and acid as active ingredients, remineralization from the depth of the demineralized layer of the tooth can be promoted, and acid resistance and remineralization can be significantly promoted. .
歯質強化剤として石化海藻と酸、または石化海藻と酸を混合処理したもの、あるいはさらにそれらにキシリトールを含有する歯質強化用の口腔用組成物としては、練り歯磨，粉歯磨又は液状歯磨き等の歯磨類、洗口剤、歯肉マッサージクリーム、うがい用錠剤又はトローチ等があげられる。 As a tooth strengthening agent, a mixture of fossilized seaweed and acid, or fossilized seaweed and acid, or a composition for oral enhancement containing xylitol therein, toothpaste, powder toothpaste or liquid toothpaste, etc. Toothpastes, mouthwashes, gum massage creams, gargle tablets or lozenges.
また、歯質強化剤として石化海藻と酸、または石化海藻と酸を混合処理したもの、あるいはさらにそれらにキシリトールを含有する歯質強化用飲食物としては、チューインガム，キャンディ，錠菓，グミゼリー，チョコレート，ビスケット又はスナック等の菓子、アイスクリーム，シャーベット又は氷菓等の冷菓、飲料、パン、ホットケーキ、乳製品、ハム，ソーセージ等の畜肉製品類、カマボコ，チクワ等の魚肉製品、惣菜類、プリン、スープ又はジャム等が挙げられるが、口腔内に長く滞留することから本発明の効果が強く認められるチューインガム、キャンディ、錠菓が好ましい。 In addition, chewing gum, candy, tablet confectionery, gummy jelly, and chocolate as tooth-enhancing foods and drinks that contain a mixture of fossilized seaweed and acid, or fossilized seaweed and acid, or further contain xylitol. , Confectionery such as biscuits or snacks, frozen confectionery such as ice cream, sorbet or ice confectionery, beverages, bread, hot cakes, livestock products such as dairy products, ham and sausages, fish products such as seaweeds and chikuwa, side dishes, pudding, Examples include soup or jam, but chewing gum, candy, and tablet confectionery, which strongly retain the effect of the present invention because they stay in the oral cavity for a long time, are preferable.
口腔用組成物への石化海藻の添加量としては、好ましくは０．１〜２０．０重量％、さらに好ましくは０．５〜１５重量％が好適である。酸の添加量としては、石化海藻1重量部に対し、好ましくは０．１〜１０重量部、さらに好ましくは０．３〜３重量部が好適である。また、石化海藻と併用するキシリトールの添加量は、利用する口腔用組成物種類や形態等により一概に決めることは困難であるが、好ましくは１〜９５重量％、さらに好ましくは１０〜７０重量％である。 The amount of petrochemical seaweed added to the oral composition is preferably 0.1 to 20.0% by weight, more preferably 0.5 to 15% by weight. The addition amount of the acid is preferably 0.1 to 10 parts by weight, more preferably 0.3 to 3 parts by weight with respect to 1 part by weight of the petrified seaweed. The amount of xylitol used in combination with fossilized seaweed is difficult to determine in general depending on the type and form of oral composition to be used, but is preferably 1 to 95% by weight, more preferably 10 to 70% by weight. It is.
飲食物への石化海藻の添加量としては、好ましくは０．１〜６．０重量％、さらに好ましくは０．５〜３重量％が好適である。酸の添加量としては、石化海藻１重量部に対し、好ましくは０．１〜１０重量部、さらに好ましくは０．３〜３重量部が好適である。また、石化海藻と併用するキシリトールの添加量は、利用する飲食物の種類や形態等により一概に決めることは困難であるが、好ましくは１〜９５重量％、さらに好ましくは１０〜７０重量％である。 The amount of petrochemical seaweed added to food and drink is preferably 0.1 to 6.0% by weight, more preferably 0.5 to 3% by weight. The amount of acid added is preferably 0.1 to 10 parts by weight, more preferably 0.3 to 3 parts by weight, per 1 part by weight of petrochemical seaweed. Further, the amount of xylitol used in combination with fossilized seaweed is difficult to determine in general depending on the type and form of food and drink to be used, but is preferably 1 to 95% by weight, more preferably 10 to 70% by weight. is there.
上述した本発明の口腔用組成物及び飲食物において、歯質強化剤を添加せしめる方法としては、石化海藻と酸を各別に、または石化海藻と酸をあらかじめ混合処理したものを口腔用組成物あるいは飲食物に添加してもよく、さらには製造過程のいかなる時に添加し、また、残余の原料と混合してもよい。なお、石化海藻と酸とキシリトールを併用する場合には、これらを予め混合して、当該口腔用組成物あるいは飲食物に添加してもよいし、各別に添加してもよい。 In the composition for oral cavity and food and drink of the present invention described above, as a method for adding a tooth enhancer, a composition obtained by mixing a petrochemical seaweed and an acid separately, or a premixed process of a petrochemical seaweed and an acid, or an oral composition or It may be added to food and drink, and may be added at any time during the production process, and may be mixed with the remaining raw materials. In addition, when using together petrified seaweed, an acid, and a xylitol, these may be mixed previously and may be added to the said composition for oral cavity, food-drinks, or may be added separately.
以下に本発明実施例について説明するが、本発明の範囲がこれらによって限定されるものではない。 Examples of the present invention will be described below, but the scope of the present invention is not limited thereto.
〔実施例１〕 酸添加による石化海藻からのフッ素溶出性
石化海藻中のフッ素量をアリザリンコンプレキソン吸光光度法によって調べたところ、１０１４ｐｐｍであった。石化海藻０．１％水溶液を調製し、クエン酸を添加後、フッ素イオン電極（オリオン社）を用い、フッ素イオン濃度を測定した。（溶液フッ素濃度）／（石化海藻中のフッ素量ｘ０．1／１００）ｘ１００を可溶化率（％）とした。
[Example 1] Fluorine elution from acidified seaweed by addition of acid The amount of fluorine in the fossilized seaweed was examined by alizarin complexone spectrophotometry and found to be 1014 ppm. A 0.1% aqueous petrochemical seaweed was prepared, citric acid was added, and the fluoride ion concentration was measured using a fluoride ion electrode (Orion). (Solution fluorine concentration) / (fluorine amount in petrochemical seaweed x 0.1 / 100) x 100 was defined as the solubilization rate (%).
その結果、石化海藻は酸の添加により、溶液中へのフッ素の溶出性が約８倍向上した。 As a result, the petrified seaweed improved the elution of fluorine into the solution by about 8 times by the addition of acid.
〔実施例２〕 石化海藻と酸との混合処理物のフッ素溶出性
石化海藻１０ｇを１リットルの水に懸濁した溶液に、オートマチックタイトレーター（ＫＹＯＴＯ ＥＬＥＣＴＲＯＮＩＣＳ社製）を用い、ｐＨを７．０に設定し、１規定の塩酸を約１７０ミリリットル加え石化海藻を溶解した。石化海藻溶解液を濾過し、ろ液を凍結乾燥し、あらかじめ石化海藻と酸を混合した処理物（酸可溶化石化海藻）を得た。
[Example 2] Fluorine elution of a mixed processed product of fossilized seaweed and acid A solution obtained by suspending 10 g of fossilized seaweed in 1 liter of water using an automatic titrator (manufactured by KYOTO ELECTRONICS Co., Ltd.) has a pH of 7.0. And about 170 ml of 1N hydrochloric acid was added to dissolve the fossilized seaweed. The solution of the petrochemical seaweed was filtered, and the filtrate was freeze-dried to obtain a processed product (acid-soluble fossilized seaweed) in which the petrochemical seaweed and acid were mixed in advance.
石化海藻および、酸可溶化石化海藻中のフッ素量をアリザリンコンプレキソン吸光光度法によって調べたところ、石化海藻中のフッ素量が１０１４ｐｐｍ、酸可溶化石化海藻中のフッ素量が９２０ｐｐｍであった。これら０．５％水溶液を調製し、フッ素イオン電極（オリオン社）を用い、フッ素イオン濃度を測定した。（溶液フッ素濃度）／（粉体フッ素含量ｘ０．５／１００）ｘ１００を可溶化率（％）とした。 When the amount of fluorine in fossilized seaweed and acid-soluble fossilized seaweed was examined by alizarin complexone spectrophotometry, the amount of fluorine in fossilized seaweed was 1014 ppm and the amount of fluorine in acid-soluble fossilized seaweed was 920 ppm. These 0.5% aqueous solutions were prepared, and the fluorine ion concentration was measured using a fluorine ion electrode (Orion). (Solution fluorine concentration) / (powder fluorine content x 0.5 / 100) x 100 was defined as the solubilization rate (%).
以上より、石化海藻の酸処理により、水へのフッ素溶出性が約８倍向上した。 From the above, the acid treatment of petrified seaweed improved the fluorine elution to water by about 8 times.
〔実施例３〕 再石灰化効果確認試験
実施例２で調製した酸可溶化石化海藻を使用した。
[Example 3] Remineralization effect confirmation test The acid-soluble fossilized seaweed prepared in Example 2 was used.
対照フッ素剤として、フッ化ナトリウム（和光純薬）、蛍石（フッ化カルシウム）（和光純薬）を使用した。 Sodium fluoride (Wako Pure Chemical) and fluorite (calcium fluoride) (Wako Pure Chemical) were used as control fluorine agents.
歯科学報Ｖｏｌ．８９，Ｎｏ．９，１４４１〜１４５５（１９８９）．に記載されているヒト抜去歯を用いる再石灰化促進効果確認試験による方法を参考に次のとおり行った。ヒト抜去歯エナメル質ブロックの表面を、３×４ｍｍの窓を残して、全体をスティッキーワックスで被覆し、これを、５０℃に加温した０．０１Ｍ酢酸・酢酸ナトリウム緩衝液（ｐＨ４．０）に２日間浸漬し、脱灰層を形成させた。その後、窓の半分をワックスで被覆し試験用歯牙エナメル質ブロックを調製した。 Dentistry Bulletin Vol. 89, no. 9, 1441-1455 (1989). The remineralization promoting effect confirmation test using human extracted teeth described in 1 was performed as follows with reference to the method. The surface of the human extracted tooth enamel block was covered with sticky wax, leaving a 3 × 4 mm window, and this was heated to 50 ° C. in 0.01 M acetic acid / sodium acetate buffer (pH 4.0). For 2 days to form a demineralized layer. Thereafter, half of the window was covered with wax to prepare a test tooth enamel block.
再石灰化処理は、１ｍＭ ＣａＣｌ２、０．６ｍＭ ＫＨ２ＰＯ４、１００ｍＭ ＮａＣｌを含み、５０ｍＭ ＫＯＨ溶液で、ｐＨ７．３に調製した再石灰化溶液（１）とその再石灰化溶液を用いて次の８種類の再石灰化溶液（２〜９）を用意し、それを３７℃とし、かつ、その各々に試験用歯牙エナメル質ブロック各１個を２週間浸漬した。ただし、各再石灰化溶液は２日置きに新しい溶液に交換した。 The remineralization treatment uses a remineralization solution (1) containing 1 mM CaCl 2 , 0.6 mM KH 2 PO 4 , 100 mM NaCl and adjusted to pH 7.3 with a 50 mM KOH solution and the remineralization solution. The following eight kinds of remineralization solutions (2 to 9) were prepared, and the temperature was set to 37 ° C., and each of the test tooth enamel blocks was immersed in each of them for 2 weeks. However, each remineralization solution was replaced with a new solution every two days.
１：再石灰化溶液
２：石化海藻を０．１重量％含んだ再石灰化溶液
３：キシリトールを５重量％含んだ再石灰化溶液
４：フッ化ナトリウムをフッ素として１.０ｐｐｍ含んだ再石灰化溶液
５：フッ化カルシウムに塩酸を加え溶解し、溶解液をフッ素として１．０ｐｐｍとなるように添加した再石灰化溶液
６：本発明の歯質強化剤として、石化海藻を０．１重量％とクエン酸を０．１重量％含んだ再石灰化溶液
７：本発明の歯質強化剤として、石化海藻を０．１重量％とクエン酸を０．１重量％、キシリトールを５重量％含んだ再石灰化液
８：本発明の歯質強化剤として、実施例２の酸可溶化石化海藻を０．１重量％含んだ再石灰化溶液
９：本発明の歯質強化剤として、実施例２の酸可溶化石化海藻を０．１重量％、キシリトールを５重量％含んだ再石灰化溶液
1: Remineralization solution 2: Remineralization solution containing 0.1% by weight of fossilized seaweed 3: Remineralization solution containing 5% by weight of xylitol 4: Recalcification containing 1.0ppm of sodium fluoride as fluorine 5: Recalcification solution in which hydrochloric acid was added to calcium fluoride and dissolved, and the solution was added to a concentration of 1.0 ppm as fluorine. 6: 0.1 wt. 7: 0.1% by weight and 0.1% by weight of citric acid 7: As a dentifrice enhancer of the present invention, 0.1% by weight of petrochemical seaweed, 0.1% by weight of citric acid and 5% by weight of xylitol Included remineralization solution 8: Recalcification solution containing 0.1% by weight of the acid-solubilized fossilized seaweed of Example 2 as a tooth strengthening agent of the present invention 9: Implemented as a tooth strengthening agent of the present invention 0.1% by weight of acid-soluble fossilized seaweed of Example 2 and 5% by weight of xylitol Remineralization solution containing
尚、本発明の歯質強化剤を使用した再石灰化溶液６，７，８，９のフッ素濃度は、実施例１及び２の結果より、ほぼ１ｐｐｍとなる。 From the results of Examples 1 and 2, the fluorine concentration of the remineralization solutions 6, 7, 8, and 9 using the tooth strengthening agent of the present invention is approximately 1 ppm.
再石灰化処理後、各試験用歯牙エナメル質ブロックのワックスを除去、ポリエステル樹脂（Ｒｅｇｏｌａｃ樹脂）で包埋し、厚さ１００μｍの研磨切片を作製し、コンタクトマイクロラジオグラムを撮影した。撮影条件は１０ｋＶ、３ｍＡ、照射時間３０分間とし、基準としてアルミ箔ステップウェッジと同時に撮影した。現像は通法に準じ行った。 After the remineralization treatment, the wax of each test tooth enamel block was removed, embedded in a polyester resin (Regolac resin), a 100 μm thick polished section was prepared, and a contact microradiogram was photographed. The shooting conditions were 10 kV, 3 mA, and the irradiation time was 30 minutes, and shooting was performed simultaneously with the aluminum foil step wedge as a reference. Development was performed according to the customary law.
また、マイクロラジオグラフィー（ＭＲ）による結果の再石灰化度を、視覚的に、次の５段階で評価することとした。 In addition, the recalcification degree as a result of microradiography (MR) was visually evaluated in the following five stages.
・再石灰化度０：エナメル質脱灰層に石灰化が認められない。
・再石灰化度１：エナメル質脱灰表層で、かすかに再石灰化が認められる。
・再石灰化度２：エナメル質脱灰表層で、比較的強い再石灰化が認められる。又はエナメル質脱灰表層及び深層で再石灰化が認められる。
・再石灰化度３：エナメル質脱灰表層から深層にかけて全体的に再石灰化が認められる。又は、エナメル質脱灰表層で強い再石灰化が認められる。
・再石灰化度４：エナメル質脱灰表層で、強い再石灰化が認められる。なおかつ、表層から深層にかけて全体的に再石灰化が認められる。
・再石灰化度５：エナメル質脱灰表層から深層にかけて全体的に強い再石灰化が認められる。
-Remineralization degree 0: Calcification is not recognized in the enamel demineralized layer.
-Remineralization degree 1: Remineralization is slightly observed in the enamel demineralized surface layer.
-Remineralization degree 2: Remineralization is relatively strong in the enamel demineralized surface layer. Or remineralization is recognized in the enamel demineralized surface layer and the deep layer.
-Remineralization degree 3: Remineralization is recognized as a whole from the enamel demineralized surface layer to the deep layer. Alternatively, strong remineralization is observed on the enamel demineralized surface layer.
-Remineralization degree 4: Strong remineralization is recognized in the enamel demineralized surface layer. In addition, remineralization is observed as a whole from the surface layer to the deep layer.
-Remineralization degree 5: Strong remineralization is recognized from the enamel demineralized surface layer to the deep layer as a whole.
以下、上記実験の結果について述べる。 The results of the above experiment will be described below.
ＭＲの結果を表３に示す。 Table 3 shows the MR results.
脱灰直後のＭＲでは、再石灰化が認められなかった（再石灰化度０）。試験用歯エナメル質ブロックに脱灰層を形成させた後、再石灰化溶液１で処理した場合には、脱灰面全体に、ゆるやかな再石灰化が認められた（再石灰化度１）。同じく試験用歯エナメル質ブロックに脱灰層を形成させた後、再石灰化溶液２で処理した場合には、脱灰面全体に、ゆるやかな再石灰化が認められた（再石灰化度１）。再石灰化溶液３で処理した場合には、エナメル質脱灰表層だけでなく、深層にも再石灰化が認められた（再石灰化度２）。再石灰化溶液４および再石灰化溶液５で処理した場合には、エナメル質脱灰表層で強い再石灰化が認められた（再石灰化度３）。同じく試験用歯エナメル質ブロックに脱灰層を形成させた後、再石灰化溶液６および８で処理した場合には、エナメル質脱灰表層に強い再石灰化が、なおかつ表層から深層にかけて全体的に再石灰化が認められた（再石灰化度４）。同じく試験用歯エナメル質ブロックに脱灰層を形成させた後、再石灰化溶液７及び９で処理した場合には、エナメル質脱灰表層及び深層の全体に強い再石灰化が認められた（再石灰化度５）。 Remineralization was not observed in the MR immediately after decalcification (degree of remineralization 0). When a demineralized layer was formed on the test tooth enamel block and then treated with the remineralization solution 1, mild remineralization was observed on the entire demineralized surface (remineralization degree 1). . Similarly, when a demineralized layer was formed on the test tooth enamel block and then treated with the remineralization solution 2, gentle remineralization was observed on the entire demineralized surface (remineralization degree 1). ). When treated with the remineralization solution 3, remineralization was observed not only in the enamel demineralized surface layer but also in the deep layer (degree of remineralization 2). When treated with the remineralization solution 4 and the remineralization solution 5, strong remineralization was observed in the enamel demineralized surface layer (degree of remineralization 3). Similarly, when a demineralized layer was formed on the test tooth enamel block and then treated with the remineralization solutions 6 and 8, the enamel demineralized surface layer had strong remineralization, and the entire surface layer to deep layer. Remineralization was observed (remineralization degree 4). Similarly, when a demineralized layer was formed on the test tooth enamel block and then treated with the remineralization solutions 7 and 9, strong remineralization was observed throughout the enamel demineralized surface layer and the deep layer ( Remineralization degree 5).
以上によれば、本発明の石化海藻と酸の組み合わせおよび実施例２の酸可溶化石化海藻（混合処理物）に再石灰化促進効果が認められた。さらに本発明の石化海藻と酸の組み合わせおよび実施例２の酸可溶化石化海藻は、再石灰化溶液に溶かしたときに同じフッ素濃度になるフッ化ナトリウム、蛍石（フッ化カルシウム）溶液と比較し、より強い再石灰化促進効果があることが確認され、しかも、石化海藻と酸の組み合わせおよび実施例２の酸可溶化石化海藻にキシリトールを併用することにより、その再石灰化促進効果が顕著に高まることが認められた。 According to the above, the remineralization promoting effect was recognized in the combination of the fossilized seaweed and acid of the present invention and the acid-solubilized fossilized seaweed (mixed processed product) of Example 2. Furthermore, the combination of the fossilized seaweed and acid of the present invention and the acid-solubilized fossilized seaweed of Example 2 are compared with sodium fluoride and fluorite (calcium fluoride) solutions that have the same fluorine concentration when dissolved in the remineralized solution. In addition, it has been confirmed that there is a stronger remineralization promoting effect, and the remineralization promoting effect is remarkable by using xylitol together with the combination of fossilized seaweed and acid and the acid-solubilized fossilized seaweed of Example 2. It was recognized that it would increase.
〔実施例４〕 歯牙エナメル質の耐酸性向上効果確認試験１
Ｃａｒｉｅｓ Ｒｅｓｅａｒｃｈ ３８：５５１〜５５６，２００４に記載されているヒト抜去歯を用いる耐酸性向上効果確認試験による方法を参考に次のとおり行った。ヒト抜去歯エナメル質ブロックの表面を、３×４ｍｍの窓を残して、全体をスティッキーワックスで被覆し、これを、３７℃に加温した、５００ｍｇ／ｌのハイドロキシアパタイト、２０ｇ／ｌのカルボポール、０．１Ｍ乳酸を含む脱灰溶液（ｐＨ４．８）に２日間浸漬し、脱灰層を形成させた。その後、窓の１／３をワックスで被覆し試験用歯牙エナメル質ブロックを調製した。
[Example 4] Acid resistance improvement effect confirmation test 1 of tooth enamel
Carrying out the acid resistance improvement effect confirmation test using human extracted teeth described in Caries Research 38: 551-556, 2004 was performed as follows. The surface of the human extracted tooth enamel block was covered with sticky wax, leaving a 3 × 4 mm window, and this was heated to 37 ° C., 500 mg / l hydroxyapatite, 20 g / l carbopol And immersed in a decalcification solution (pH 4.8) containing 0.1 M lactic acid for 2 days to form a demineralization layer. Thereafter, 1/3 of the window was covered with wax to prepare a test tooth enamel block.
再石灰化処理は、１ｍＭ ＣａＣｌ２、０．６ｍＭ ＫＨ２ＰＯ４、１００ｍＭ ＮａＣｌを含み、５０ｍＭ ＫＯＨ溶液で、ｐＨ７．３に調製した再石灰化溶液を用いて、実施例３の９種類の再石灰化溶液を用意し、それを３７℃とし、かつ、その各々に試験用歯牙エナメル質ブロック各１個を２週間浸漬した。ただし、各再石灰化溶液は２日おきに新しい溶液に交換した。 The remineralization treatment was carried out using 9 types of remineralization of Example 3 using a remineralization solution containing 1 mM CaCl 2 , 0.6 mM KH 2 PO 4 , 100 mM NaCl, and adjusted to pH 7.3 with a 50 mM KOH solution. A calcification solution was prepared, which was set to 37 ° C., and each test tooth enamel block was immersed in each for 2 weeks. However, each remineralization solution was replaced with a new solution every two days.
再石灰化処理後、窓の半分をワックスで被覆し、耐酸性試験用歯牙エナメル質ブロックを調製した。耐酸性処理は、上記３７℃に加温した脱灰溶液に２日間浸漬した。 After the remineralization treatment, half of the window was covered with wax to prepare a tooth enamel block for acid resistance test. The acid resistance treatment was immersed in the decalcification solution heated to 37 ° C. for 2 days.
耐酸性処理後、各試験用歯牙エナメル質ブロックのワックスを除去、ポリエステル樹脂（Ｒｅｇｏｌａｃ樹脂）で包埋し、１００μｍの研磨切片を作製し、コンタクトマイクロラジオグラムを撮影した。撮影条件は１０ｋＶ、３ｍＡ、照射時間３０分間とし、基準としてアルミ箔ステップウェッジと同時に撮影した。現像は通法に準じ行った。 After the acid resistance treatment, the wax of each test tooth enamel block was removed, embedded in a polyester resin (Regolac resin), a 100 μm polished section was prepared, and a contact microradiogram was photographed. The shooting conditions were 10 kV, 3 mA, and the irradiation time was 30 minutes, and shooting was performed simultaneously with the aluminum foil step wedge as a reference. Development was performed according to the customary law.
また、マイクロラジオグラフィー（ＭＲ）による結果の石灰化度を、視覚的に、次の５段階で評価することとした。 In addition, the degree of calcification as a result of microradiography (MR) was visually evaluated in the following five stages.
・石灰化度１：エナメル質表層から深層にかけて全体的に強い脱灰が認められる。
・石灰化度２：エナメル質表層から深層にかけて全体的に脱灰が認められる。
・石灰化度３：エナメル質表層または深層で比較的弱い脱灰が認められる。
・石灰化度４：エナメル質層にかすかに脱灰が認められる。
・石灰化度５： エナメル質層にほとんど脱灰が認められない。
-Calcification degree 1: Strong demineralization is recognized as a whole from the enamel surface layer to the deep layer.
Degree of calcification 2: Demineralization is observed from the surface layer of enamel to the deep layer.
-Calcification degree 3: Relatively weak demineralization is observed in the enamel surface layer or deep layer.
Degree of calcification 4: Demineralization is slightly observed in the enamel layer.
・ Degree of calcification 5: Almost no decalcification is observed in the enamel layer.
試験用歯エナメル質ブロックを脱灰溶液に浸漬した直後のマイクロラジオグラフィーの結果、再石灰化溶液に浸漬した直後のマイクロラジオグラフィーの結果、再度脱灰液浸漬後のマイクロラジオグラフィーの結果を表４に示す。 The results of microradiography immediately after immersing the test tooth enamel block in the decalcification solution, the results of microradiography immediately after immersing in the remineralization solution, and the results of microradiography after immersion in the decalcification solution are shown. 4 shows.
以上によれば、本発明の石化海藻と酸の組み合わせおよび実施例２の酸可溶化石化海藻（混合処理物）は、再石灰化溶液１や、再石灰化溶液に溶かしたときに同じフッ素濃度になるフッ化ナトリウム、蛍石（フッ化カルシウム）と比較し、より強い歯牙エナメル質の耐酸性を向上する効果があることが確認され、しかも、石化海藻と酸の組み合わせおよび実施例２の酸可溶化石化海藻にキシリトールを併用することにより、その耐酸性向上効果が顕著に高まることが認められた。 According to the above, the combination of the fossilized seaweed and acid of the present invention and the acid-solubilized fossilized seaweed (mixed product) of Example 2 have the same fluorine concentration when dissolved in the remineralized solution 1 or the remineralized solution. Compared with sodium fluoride and fluorite (calcium fluoride), it is confirmed that there is an effect of improving the acid resistance of stronger tooth enamel, and the combination of fossilized seaweed and acid and the acid of Example 2 It was recognized that the acid resistance improvement effect was remarkably enhanced by using xylitol together with the soluble fossilized seaweed.
〔実施例５〕 歯牙エナメル質の耐酸性向上効果確認試験２
ヒト抜去歯エナメル質ブロックの表面を、３×４ｍｍの窓を残して、全体をスティッキーワックスで被覆した。
[Example 5] Acid resistance improvement effect confirmation test 2 of tooth enamel
The surface of the human extracted tooth enamel block was covered entirely with sticky wax, leaving a 3 × 4 mm window.
試験溶液処理は、１ｍＭ ＣａＣｌ２、０．６ｍＭ ＫＨ２ＰＯ４、１００ｍＭ ＮａＣｌを含み、５０ｍＭ ＫＯＨ溶液で、ｐＨ７．３に調製した試験溶液を用いて、９種類の試験溶液（実施例３の９種類の再石灰化溶液と同じ）を用意し、それを３７℃とし、かつ、その各々に試験用歯牙エナメル質ブロック各１個を２週間浸漬した。ただし、各試験溶液は２日置きに新しい溶液に交換した。 The test solution treatment was performed using nine test solutions (9 in Example 3) using test solutions containing 1 mM CaCl 2 , 0.6 mM KH 2 PO 4 , 100 mM NaCl, and adjusted to pH 7.3 with a 50 mM KOH solution. The same kind of remineralization solution) was prepared, and the temperature was set to 37 ° C., and each of the test tooth enamel blocks was immersed in each for 2 weeks. However, each test solution was replaced with a new solution every two days.
尚、本試験においては、上記した９種類の試験溶液には前もって浸漬しない未処理の歯牙エナメル質ブロックについても、以下の試験を行った。 In this test, the following test was also performed on an untreated tooth enamel block that was not previously immersed in the above-described nine test solutions.
試験溶液浸漬後、３７℃に加温した０．１Ｍ乳酸を含む脱灰溶液（ｐＨ４．５）に８時間浸漬した。 After immersion in the test solution, it was immersed in a decalcification solution (pH 4.5) containing 0.1 M lactic acid heated to 37 ° C. for 8 hours.
脱灰処理後、各試験用歯牙エナメル質ブロックのワックスを除去、ポリエステル樹脂（Ｒｅｇｏｌａｃ樹脂）で包埋し、厚さ１００μｍの研磨切片を作製し、コンタクトマイクロラジオグラムを撮影した。撮影条件は１０ｋＶ、３ｍＡ、照射時間３０分間とし、基準としてアルミ箔ステップウェッジと同時に撮影した。現像は通法に準じ行った。試験溶液を使用した試料の比較対照として、未処理のエナメル質ブロックを用いた。 After the decalcification treatment, the wax of each test tooth enamel block was removed, embedded in a polyester resin (Regolac resin), a 100 μm thick polished section was prepared, and a contact microradiogram was photographed. The shooting conditions were 10 kV, 3 mA, and the irradiation time was 30 minutes, and shooting was performed simultaneously with the aluminum foil step wedge as a reference. Development was performed according to the customary law. An untreated enamel block was used as a control for the sample using the test solution.
また、マイクロラジオグラフィー（ＭＲ）による結果の脱灰度を、視覚的に、次の５段階で評価することとした。 Further, the degree of decalcification as a result of microradiography (MR) was visually evaluated in the following five stages.
・脱灰度０：エナメル質層にほとんど脱灰が認められない。
・脱灰度１：エナメル質層にかすかに脱灰が認められる。
・脱灰度２：エナメル質層表層または深層で脱灰が認められる。
・脱灰度３：エナメル質表層から深層にかけて全体的に脱灰が認められる。
・脱灰度４：エナメル質表層から深層にかけて全体的に強い脱灰が認められる。
Demineralization degree 0: Demineralization is hardly observed in the enamel layer.
Demineralization degree 1: Demineralization is slightly observed in the enamel layer.
Demineralization degree 2: Demineralization is observed in the surface layer or deep layer of the enamel layer.
Demineralization degree 3: Demineralization is observed as a whole from the surface layer of the enamel to the deep layer.
Demineralization degree 4: Strong demineralization is observed as a whole from the enamel surface layer to the deep layer.
試験用歯エナメル質ブロックを試験溶液に浸漬した直後のマイクロラジオグラフィーの結果、脱灰液浸漬後のマイクロラジオグラフィーの結果を表２に示す。 Table 2 shows the results of microradiography immediately after the test tooth enamel block was immersed in the test solution, and the results of microradiography after immersion in the decalcification solution.
以上によれば、本発明の石化海藻と酸の組み合わせおよび実施例２の酸可溶化石化海藻（混合処理物）は、再石灰化溶液１や、再石灰化溶液に溶かしたときに同じフッ素濃度になるフッ化ナトリウム、蛍石（フッ化カルシウム）と比較し、より強い歯牙エナメル質の耐酸性を向上する効果があることが確認され、しかも、石化海藻と酸の組み合わせおよび実施例２の酸可溶化石化海藻にキシリトールを併用することにより、その耐酸性向上効果が顕著に高まることが認められた。 According to the above, the combination of the fossilized seaweed and acid of the present invention and the acid-solubilized fossilized seaweed (mixed product) of Example 2 have the same fluorine concentration when dissolved in the remineralized solution 1 or the remineralized solution. Compared with sodium fluoride and fluorite (calcium fluoride), it is confirmed that there is an effect of improving the acid resistance of stronger tooth enamel, and the combination of fossilized seaweed and acid and the acid of Example 2 It was recognized that the acid resistance improvement effect was remarkably enhanced by using xylitol together with the soluble fossilized seaweed.
〔実施例６〕 歯牙象牙質の耐酸性向上効果確認試験
健全な人歯牙の象牙質を切削により露出させたものを試料とした。ヒト抜去歯象牙質ブロックの表面を、３×４ｍｍの窓を残して、全体をスティッキーワックスで被覆した。
[Example 6] Test for confirming effect of improving acid resistance of dental dentin A sample of a healthy human dental dentin exposed by cutting was used as a sample. The entire surface of the human extracted dentin block was covered with sticky wax, leaving a 3 × 4 mm window.
試験溶液処理は、１ｍＭ ＣａＣｌ２、０．６ｍＭ ＫＨ２ＰＯ４、１００ｍＭ ＮａＣｌを含み、５０ｍＭ ＫＯＨ溶液で、ｐＨ７．３に調製した試験溶液を用いて、実施例５の９種類の試験溶液を用意し、それを３７℃とし、かつ、その各々に試験用歯牙象牙質ブロック各１個を２週間浸漬した。ただし、各試験溶液は２日置きに新しい溶液に交換した。 For the test solution treatment, nine test solutions of Example 5 were prepared using a test solution containing 1 mM CaCl 2 , 0.6 mM KH 2 PO 4 , 100 mM NaCl, and adjusted to pH 7.3 with a 50 mM KOH solution. Then, the temperature was set to 37 ° C., and each test dentin block was immersed in each for 2 weeks. However, each test solution was replaced with a new solution every two days.
尚、本試験においては、上記した９種類の溶液には前もって浸漬しない未処理の歯牙象牙質ブロックについても、以下の試験を行った。 In this test, the following test was also performed on an untreated dental dentin block that was not previously immersed in the nine types of solutions described above.
試験溶液浸漬後、３７℃に加温した０．１Ｍ乳酸を含む脱灰溶液（ｐＨ４．５）に８時間浸漬した。 After immersion in the test solution, it was immersed in a decalcification solution (pH 4.5) containing 0.1 M lactic acid heated to 37 ° C. for 8 hours.
脱灰処理後、各試験用歯牙象牙質ブロックのワックスを除去、ポリエステル樹脂（Ｒｅｇｏｌａｃ樹脂）で包埋し、厚さ１００μｍの研磨切片を作製し、コンタクトマイクロラジオグラムを撮影した。撮影条件は１０ｋＶ、３ｍＡ、照射時間３０分間とし、基準としてアルミ箔ステップウェッジと同時に撮影した。現像は通法に準じ行った。試験溶液を使用した試料の比較対照として、未処理の象牙質ブロックを用いた。 After the decalcification treatment, the wax of each test dentin block was removed, embedded in a polyester resin (Regolac resin), a 100 μm-thick polished section was prepared, and a contact microradiogram was photographed. The shooting conditions were 10 kV, 3 mA, and the irradiation time was 30 minutes, and shooting was performed simultaneously with the aluminum foil step wedge as a reference. Development was performed according to the customary law. An untreated dentin block was used as a control for the sample using the test solution.
また、マイクロラジオグラフィー（ＭＲ）による結果の脱灰度を、視覚的に、次の５段階で評価することとした。 Further, the degree of decalcification as a result of microradiography (MR) was visually evaluated in the following five stages.
・脱灰度０：象牙質層に脱灰がほとんど認められない。
・脱灰度１：象牙質層にかすかに脱灰が認められる。
・脱灰度２：象牙質層表層または深層で脱灰が認められる。
・脱灰度３：象牙質表層から深層にかけて全体的に脱灰が認められる。
・脱灰度４：象牙質表層から深層にかけて全体的に強い脱灰が認められる。
Demineralization degree 0: Demineralization is hardly observed in the dentin layer.
Demineralization degree 1: Demineralization is slightly observed in the dentin layer.
Demineralization degree 2: Demineralization is observed at the surface layer or deep layer of the dentin layer.
Demineralization degree 3: Demineralization is observed as a whole from the dentine surface layer to the deep layer.
Demineralization degree 4: Strong demineralization is observed as a whole from the dentine surface layer to the deep layer.
試験用歯象牙質ブロックを試験溶液に浸漬した直後のマイクロラジオグラフィーの結果、脱灰液浸漬後のマイクロラジオグラフィーの結果を表６に示す。 Table 6 shows the results of microradiography immediately after the test dentin block was immersed in the test solution, and the results of microradiography after immersion of the demineralized solution.
以上によれば、本発明の石化海藻と酸の組み合わせおよび実施例２の酸可溶化石化海藻（混合処理物）は、再石灰化溶液１や、再石灰化溶液に溶かしたときに同じフッ素濃度になるフッ化ナトリウム、蛍石（フッ化カルシウム）と比較し、より強い歯牙象牙質の耐酸性を向上する効果があることが確認され、しかも、石化海藻と酸の組み合わせおよび実施例２の酸可溶化石化海藻にキシリトールを併用することにより、その耐酸性向上効果が顕著に高まることが認められた。石化海藻と酸の組み合わせおよび実施例２の酸可溶化石化海藻、さらにキシリトールを併用することにより、象牙質の耐酸性をも向上させることができるので、それによって歯根面および象牙質の齲蝕を積極的に抑制し、象牙質知覚過敏症をも予防、治療することができる。 According to the above, the combination of the fossilized seaweed and acid of the present invention and the acid-solubilized fossilized seaweed (mixed product) of Example 2 have the same fluorine concentration when dissolved in the remineralized solution 1 or the remineralized solution. Compared with sodium fluoride and fluorite (calcium fluoride), it is confirmed that there is an effect of improving the acid resistance of stronger dentin, and the combination of fossilized seaweed and acid and the acid of Example 2 It was recognized that the acid resistance improvement effect was remarkably enhanced by using xylitol together with the soluble fossilized seaweed. By combining the fossilized seaweed and acid, the acid-solubilized fossilized seaweed of Example 2, and further xylitol, it is possible to improve the acid resistance of the dentin, thereby actively promoting caries on the root surface and dentin. It is possible to prevent and treat dentin hypersensitivity.
〔実施例７〕（チューインガム）
酸可溶化石化海藻とキシリトールを使用し、表７の配合による実施例７及び比較例１のチューインガムを作製した。
[Example 7] (chewing gum)
Using the acid-soluble fossilized seaweed and xylitol, the chewing gums of Example 7 and Comparative Example 1 having the composition shown in Table 7 were prepared.
チューインガムの有効成分の抽出操作は、「食品および代用糖の齲蝕誘発性を総合的に評価するための基礎的研究（課題番号０４３０４０４５）平成５年度科学研究費補助金研究成果報告書（研究代表者 山田 正）；ｐ８６〜８９」を参考にして行った。 The extraction operation of the active ingredients of chewing gum is as follows: “Basic research for comprehensive evaluation of caries-inducing properties of foods and sugar substitutes (issue number 04304045)” Tadashi Yamada); p86-89 ".
上記実施例７及び比較例１のチューインガムについて、それを細片化して１０ｇを秤量した。その各々に、実施例３の場合と同じ組成の再石灰化溶液（６０℃）５０ｍｌを加え、ガラス棒でよく擂り潰して含有成分を溶出し、それに、さらに上記再石灰化溶液（６０℃）５０ｍｌを加えて再び溶出操作を行った後、遠心分離により細かいガムベースを取り除くことにより、実施例７及び比較例１のチューインガムに対応する２種類のチューインガム抽出液を得た。 About the chewing gum of the said Example 7 and the comparative example 1, it was cut into pieces and 10 g was weighed. To each of them, 50 ml of a remineralization solution (60 ° C.) having the same composition as in Example 3 was added, and the mixture was thoroughly crushed with a glass rod to elute the contained components. Further, the above remineralization solution (60 ° C.) After 50 ml was added and the elution operation was performed again, the fine gum base was removed by centrifugation to obtain two types of chewing gum extracts corresponding to the chewing gums of Example 7 and Comparative Example 1.
〔実施例８〕（チューインガム）
石化海藻とキシリトールを使用し、表８の配合による実施例８及び比較例２、３のチューインガムを作製した。
[Example 8] (chewing gum)
Chewing gums of Example 8 and Comparative Examples 2 and 3 having the composition shown in Table 8 were prepared using fossilized seaweed and xylitol.
チューインガムの有効成分の抽出操作は、「食品および代用糖の齲蝕誘発性を総合的に評価するための基礎的研究（課題番号０４３０４０４５）平成５年度科学研究費補助金研究成果報告書（研究代表者 山田 正）；ｐ８６〜８９」を参考に、実施例７、比較例１と同様の方法で行った。実施例８及び比較例２、３のチューインガムに対応する３種類のチューインガム抽出液を得た。 The extraction operation of the active ingredients of chewing gum is as follows: “Basic research for comprehensive evaluation of caries-inducing properties of foods and sugar substitutes (issue number 04304045)” The process was performed in the same manner as in Example 7 and Comparative Example 1, with reference to “Yamada, Tadashi ;; p86-89”. Three types of chewing gum extracts corresponding to the chewing gums of Example 8 and Comparative Examples 2 and 3 were obtained.
〔実施例９〕（錠菓）
酸可溶化石化海藻とキシリトールを使用し、表９の配合による実施例９及び比較例４の錠菓を作製した。
[Example 9] (Tablet confectionery)
Using the acid-soluble fossilized seaweed and xylitol, tablet confections of Example 9 and Comparative Example 4 having the composition shown in Table 9 were prepared.
錠菓の有効成分の抽出操作は、「食品および代用糖の齲蝕誘発性を総合的に評価するための基礎的研究（課題番号０４３０４０４５）平成５年度科学研究費補助金研究成果報告書（研究代表者 山田 正）；ｐ８６〜８９」を参考に、実施例７、比較例１と同様の方法で行った。実施例９及び比較例４の錠菓に対応する２種類の錠菓抽出液を得た。 The extraction operation of the active ingredients in tablet confections is as follows: “Basic research for comprehensive evaluation of caries-inducing properties of foods and sugar substitutes (issue number 04304045)” The process was carried out in the same manner as in Example 7 and Comparative Example 1 with reference to "P. 86-89". Two types of tablet confectionery extracts corresponding to the tablet confectionery of Example 9 and Comparative Example 4 were obtained.
〔実施例１０〕（錠菓）
石化海藻とキシリトールを使用し、表１０の配合による実施例１０及び比較例５、６の錠菓を作製した。
[Example 10] (Tablet confectionery)
Using fossilized seaweed and xylitol, tablet confections of Example 10 and Comparative Examples 5 and 6 having the composition shown in Table 10 were prepared.
錠菓の有効成分の抽出操作は、「食品および代用糖の齲蝕誘発性を総合的に評価するための基礎的研究（課題番号０４３０４０４５）平成５年度科学研究費補助金研究成果報告書（研究代表者 山田 正）；ｐ８６〜８９」を参考に、実施例７、比較例１と同様の方法で行った。実施例１０及び比較例５、６の錠菓に対応する３種類の錠菓抽出液を得た。 The extraction operation of the active ingredients in tablet confections is as follows: “Basic research for comprehensive evaluation of caries-inducing properties of foods and sugar substitutes (issue number 04304045)” The process was carried out in the same manner as in Example 7 and Comparative Example 1 with reference to "P. 86-89". Three types of tablet confectionary extracts corresponding to the tablet confectionery of Example 10 and Comparative Examples 5 and 6 were obtained.
〔実施例１１〕（キャンディ）
酸可溶化石化海藻とキシリトールを使用し、表１１の配合による実施例１１及び比較例７のキャンディを作製した。
[Example 11] (Candy)
Using the acid-soluble fossilized seaweed and xylitol, the candy of Example 11 and Comparative Example 7 having the composition shown in Table 11 was prepared.
キャンディの有効成分の抽出操作は、「食品および代用糖の齲蝕誘発性を総合的に評価するための基礎的研究（課題番号０４３０４０４５）平成５年度科学研究費補助金研究成果報告書（研究代表者 山田 正）；ｐ８６〜８９」を参考に、実施例７、比較例１と同様の方法で行った。実施例１１及び比較例７のキャンディに対応する２種類のキャンディ抽出液を得た。 The extraction operation of the active ingredient of candy is as follows: “Basic research for comprehensive evaluation of caries-inducing properties of foods and sugar substitutes (Project number 04304045)” The process was performed in the same manner as in Example 7 and Comparative Example 1, with reference to “Yamada, Tadashi ;; p86-89”. Two types of candy extracts corresponding to the candy of Example 11 and Comparative Example 7 were obtained.
〔実施例１２〕（キャンディ）
石化海藻とキシリトールを使用し、表１２の配合による実施例１２及び比較例８、９のキャンディを作製した。
[Example 12] (Candy)
The candy of Example 12 and the comparative examples 8 and 9 by the mixing | blending of Table 12 was produced using fossilized seaweed and xylitol.
キャンディの有効成分の抽出操作は、「食品および代用糖の齲蝕誘発性を総合的に評価するための基礎的研究（課題番号０４３０４０４５）平成５年度科学研究費補助金研究成果報告書（研究代表者 山田 正）；ｐ８６〜８９」を参考に、実施例７、比較例１と同様の方法で行った。実施例１２及び比較例８、９のキャンディに対応する３種類のキャンディ抽出液を得た。 The extraction operation of the active ingredient of candy is as follows: “Basic research for comprehensive evaluation of caries-inducing properties of foods and sugar substitutes (Project number 04304045)” The process was performed in the same manner as in Example 7 and Comparative Example 1, with reference to “Yamada, Tadashi ;; p86-89”. Three types of candy extracts corresponding to the candy of Example 12 and Comparative Examples 8 and 9 were obtained.
実施例７、８、９、１０、１１、１２、比較例１、２、３、４、５、６、７、８、９で得られた抽出液について、実施例３の方法で再石灰化促進効果を、実施例４、５、６の方法で耐酸性向上効果を評価した。各試験のマイクロラジオグラフィーの評価結果を表１３−１及び表１３−２に示す。 The extract obtained in Examples 7, 8, 9, 10, 11, 12 and Comparative Examples 1, 2, 3, 4, 5, 6, 7, 8, 9 was recalcified by the method of Example 3. The effect of improving acid resistance was evaluated by the methods of Examples 4, 5, and 6 for the promoting effect. Tables 13-1 and 13-2 show the microradiography evaluation results of each test.
以上によれば、石化海藻と酸、もしくは、酸可溶化石化海藻を配合したガム、錠菓およびキャンディが歯牙エナメル質の再石灰化促進効果、歯牙エナメル質と象牙質の耐酸性向上効果を示した。 According to the above, gum, tablet confectionery and candy containing fossilized seaweed and acid or acid-soluble fossilized seaweed show the effect of promoting remineralization of tooth enamel and the effect of improving acid resistance of tooth enamel and dentin. It was.
以下に実施例１３〜２８の配合割合（数字は重量％）を示す。 The blending ratios of Examples 13 to 28 (numbers are% by weight) are shown below.
〔実施例１３〕（チューインガム）
ガムベース ２０．０
ソルビトール ５５．０
マルチトール ２３．８
軟化剤 １．０
酸可溶化石化海藻 ０．２
[Example 13] (chewing gum)
Gum base 20.0
Sorbitol 55.0
Maltitol 23.8
Softener 1.0
Acid-soluble fossilized seaweed 0.2
〔実施例１４〕（チューインガム）
ガムベース ２０．０
キシリトール ５５．０
マルチトール ２２．５
軟化剤 １．０
クエン酸 １．０
石化海藻 ０．５
[Example 14] (chewing gum)
Gum base 20.0
Xylitol 55.0
Maltitol 22.5
Softener 1.0
Citric acid 1.0
Petrochemical seaweed 0.5
〔実施例１５〕（キャンディ）
キシリトール ４８．０
還元麦芽糖水飴 ３６．５
酸可溶化石化海藻 １．０
香料 ０．４
精製水 １４．１
[Example 15] (Candy)
Xylitol 48.0
Reduced maltose starch syrup 36.5
Acid-soluble fossilized seaweed 1.0
Fragrance 0.4
Purified water 14.1
〔実施例１６〕（キャンディ）
パラチニット ４８．０
還元麦芽糖水飴 ３６．０
酸可溶化石化海藻 ０．５
香料 ０．４
精製水 １５．１
[Example 16] (Candy)
Palatinit 48.0
Reduced maltose starch syrup 36.0
Acid-soluble fossilized seaweed 0.5
Fragrance 0.4
Purified water 15.1
〔実施例１７〕（錠菓）
キシリトール ７５．０
乳糖 ２０．９
グリセリン脂肪酸エステル ０．１
クエン酸 ０．１
石化海藻 ０．１
精製水 ３．８
[Example 17] (Tablet confectionery)
Xylitol 75.0
Lactose 20.9
Glycerin fatty acid ester 0.1
Citric acid 0.1
Petrochemical seaweed 0.1
Purified water 3.8
〔実施例１８〕（錠菓）
キシリトール ７５．０
乳糖 １２．１
グリセリン脂肪酸エステル ０．１
クエン酸 ３．０
石化海藻 ６．０
精製水 ３．８
[Example 18] (Tablet confectionery)
Xylitol 75.0
Lactose 12.1
Glycerin fatty acid ester 0.1
Citric acid 3.0
Petrified seaweed 6.0
Purified water 3.8
〔実施例１９〕（錠菓）
キシリトール ７５．０
パラチニット ２０．０
グリセリン脂肪酸エステル ０．２
酸可溶化石化海藻 ０．５
精製水 ４．３
[Example 19] (Tablet confectionery)
Xylitol 75.0
Palatinit 20.0
Glycerin fatty acid ester 0.2
Acid-soluble fossilized seaweed 0.5
Purified water 4.3
〔実施例２０〕（チョコレート）
カカオマス １５．０
全脂粉乳 ２５．０
キシリトール ４０．９
酸可溶化石化海藻 ０．５
ココアバター １８．０
乳化剤 ０．３
香料 ０．３
[Example 20] (Chocolate)
Cacaomas 15.0
Whole milk powder 25.0
Xylitol 40.9
Acid-soluble fossilized seaweed 0.5
Cocoa Butter 18.0
Emulsifier 0.3
Fragrance 0.3
〔実施例２１〕（アイスクリーム）
クリーム（脂肪率４５％） ２５．０
牛乳（脂肪率３．７％） ３５．０
脱脂粉乳（無糖） ２４．３
キシリトール １０．４
第２リン酸カルシウム ０．１
コーンシロップ ４．４
安定剤 ０．７５
酸可溶化石化海藻 ０．１５
[Example 21] (Ice cream)
Cream (fat percentage 45%) 25.0
Milk (fat percentage 3.7%) 35.0
Nonfat dry milk (sugar-free) 24.3
Xylitol 10.4
Dicalcium phosphate 0.1
Corn syrup 4.4
Stabilizer 0.75
Acid-soluble fossilized seaweed 0.15
〔実施例２２〕（飲料）
加糖ブドウ糖液糖 ０．３
キシリトール ８．６
酸味料 １．０
香料 ０．４
クエン酸 ０．３
石化海藻 ０．３
精製水 ８９．１
[Example 22] (Beverage)
Sugared glucose liquid sugar 0.3
Xylitol 8.6
Acidulant 1.0
Fragrance 0.4
Citric acid 0.3
Petrified seaweed 0.3
Purified water 89.1
〔実施例２３〕（歯磨）
水酸化アルミニウム ３５．０
無水ケイ酸 １５．０
キシリトール １０．０
ラウリル硫酸ナトリウム １．０
香料 ０．５
酸可溶化石化海藻 ０．５
精製水 ３８．０
[Example 23] (Dental brushing)
Aluminum hydroxide 35.0
Silicic anhydride 15.0
Xylitol 10.0
Sodium lauryl sulfate 1.0
Fragrance 0.5
Acid-soluble fossilized seaweed 0.5
Purified water 38.0
〔実施例２４〕（歯磨）
水酸化アルミニウム １５．０
無水ケイ酸 １５．０
キシリトール １０．０
ラウリル硫酸ナトリウム １．０
香料 ０．５
酸可溶化石化海藻 ２０．０
精製水 ３８．５
[Example 24] (Dental brushing)
Aluminum hydroxide 15.0
Silicic anhydride 15.0
Xylitol 10.0
Sodium lauryl sulfate 1.0
Fragrance 0.5
Acid-soluble fossilized seaweed 20.0
Purified water 38.5
〔実施例２５〕（洗口剤）
キシリトール ２０．０
グリセリン １０．０
ラウリル硫酸ナトリウム １．０
香料 ０．２
酸可溶化石化海藻 １．０
精製水 ６７．８
[Example 25] (Mouthwash)
Xylitol 20.0
Glycerin 10.0
Sodium lauryl sulfate 1.0
Fragrance 0.2
Acid-soluble fossilized seaweed 1.0
Purified water 67.8
〔実施例２６〕（洗口剤）
キシリトール ７．４
酸可溶化石化海藻 ０．５
グリセリン １０．０
ラウリル硫酸ナトリウム １．５
香料 ０．４
精製水 ８０．２
[Example 26] (Mouthwash)
Xylitol 7.4
Acid-soluble fossilized seaweed 0.5
Glycerin 10.0
Sodium lauryl sulfate 1.5
Fragrance 0.4
Purified water 80.2
〔実施例２７〕（洗口剤）
ソルビトール ７．４
グリセリン １０．０
ラウリル硫酸ナトリウム １．５
香料 ０．６
酸可溶化石化海藻 ０．５
精製水 ８０．０
[Example 27] (Mouthwash)
Sorbitol 7.4
Glycerin 10.0
Sodium lauryl sulfate 1.5
Fragrance 0.6
Acid-soluble fossilized seaweed 0.5
Purified water 80.0
〔実施例２８〕（洗口剤）
キシリトール １０．０
グリセリン １０．０
ラウリル硫酸ナトリウム １．０
香料 ０．２
酸可溶化石化海藻 ２０．０
精製水 ５８．８
[Example 28] (Mouthwash)
Xylitol 10.0
Glycerin 10.0
Sodium lauryl sulfate 1.0
Fragrance 0.2
Acid-soluble fossilized seaweed 20.0
Purified water 58.8
Claims (17)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006312310A JP5053624B2 (en) | 2006-11-18 | 2006-11-18 | Tooth enhancer and method for producing the same |
| KR1020070118160A KR100963619B1 (en) | 2006-11-18 | 2007-11-19 | Teeth strengthening agent, composition and food products for oral cavity containing that, and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006312310A JP5053624B2 (en) | 2006-11-18 | 2006-11-18 | Tooth enhancer and method for producing the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008127304A true JP2008127304A (en) | 2008-06-05 |
| JP5053624B2 JP5053624B2 (en) | 2012-10-17 |
Family
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| Application Number | Title | Priority Date | Filing Date |
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| JP2006312310A Expired - Fee Related JP5053624B2 (en) | 2006-11-18 | 2006-11-18 | Tooth enhancer and method for producing the same |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP5053624B2 (en) |
| KR (1) | KR100963619B1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016043524A1 (en) * | 2014-09-16 | 2016-03-24 | 주식회사 메디바이오랩 | Composition for preventing or alleviating periodontal diseases, containing, as active ingredient, mangosteen extract or α- or γ-mangosteen |
| JP2020059689A (en) * | 2018-10-09 | 2020-04-16 | 炭プラスラボ株式会社 | Oral composition |
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| JPS63500778A (en) * | 1985-12-03 | 1988-03-24 | エ−ウェルス，ロルフ | porous hydroxyl apatite material |
| JPH0833463A (en) * | 1994-07-25 | 1996-02-06 | Micro Arujie Corp Kk | Health food containing calsium as main ingredient |
| JPH09272868A (en) * | 1996-04-03 | 1997-10-21 | Hiroshi Shimada | New soil modifier for calcium-rich crop |
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| JP3689803B2 (en) * | 1998-10-20 | 2005-08-31 | 株式会社ロッテ | Tooth enamel remineralization accelerator, oral composition and food and drink |
| KR100379641B1 (en) * | 2000-11-27 | 2003-04-10 | 롯데제과주식회사 | Composition and Foodstuffs for Dental Hygiene |
| KR100445994B1 (en) * | 2002-01-21 | 2004-08-25 | 롯데제과주식회사 | A promoting composition for re-calcification of teeth |
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2006
- 2006-11-18 JP JP2006312310A patent/JP5053624B2/en not_active Expired - Fee Related
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- 2007-11-19 KR KR1020070118160A patent/KR100963619B1/en active IP Right Grant
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63500778A (en) * | 1985-12-03 | 1988-03-24 | エ−ウェルス，ロルフ | porous hydroxyl apatite material |
| JPH0833463A (en) * | 1994-07-25 | 1996-02-06 | Micro Arujie Corp Kk | Health food containing calsium as main ingredient |
| JPH09272868A (en) * | 1996-04-03 | 1997-10-21 | Hiroshi Shimada | New soil modifier for calcium-rich crop |
| JP2000053549A (en) * | 1998-08-06 | 2000-02-22 | Lotte Co Ltd | Agent for accelerating recalcification of dental enamel, composition for oral cavity, and food and drink |
| JP2002167318A (en) * | 2000-11-30 | 2002-06-11 | Lion Corp | Solid dental composition |
| JP2003146836A (en) * | 2001-11-09 | 2003-05-21 | Katakura Chikkarin Co Ltd | Cosmetic |
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| JP2006016354A (en) * | 2004-07-02 | 2006-01-19 | Ina Food Ind Co Ltd | Oral care product |
| JP2006241122A (en) * | 2005-03-07 | 2006-09-14 | Sunstar Inc | Recalcification-promoting composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016043524A1 (en) * | 2014-09-16 | 2016-03-24 | 주식회사 메디바이오랩 | Composition for preventing or alleviating periodontal diseases, containing, as active ingredient, mangosteen extract or α- or γ-mangosteen |
| JP2017529400A (en) * | 2014-09-16 | 2017-10-05 | メディ バイオ ラブ コーポレイション リミテッドＭｅｄｉ Ｂｉｏ Ｌａｂ． Ｃｏ．， Ｌｔｄ． | Mangosteen extract or composition for preventing or ameliorating periodontal disease comprising alpha and gamma mangosteen as active ingredients |
| US10022412B2 (en) | 2014-09-16 | 2018-07-17 | Medi Bio Lab. Co., Ltd. | Composition for preventing or alleviating periodontal diseases, containing, as active ingredient, mangosteen extract or α- or γ-mangosteen |
| JP2020059689A (en) * | 2018-10-09 | 2020-04-16 | 炭プラスラボ株式会社 | Oral composition |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20080045081A (en) | 2008-05-22 |
| KR100963619B1 (en) | 2010-06-15 |
| JP5053624B2 (en) | 2012-10-17 |
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