JP2008122372A5 - - Google Patents

Claims (17)

In simple membrane assay method comprising detecting or quantifying the presence of the detected object in the test body on the assay device which includes a membrane capture reagent is bound to capture the object to be detected, specimen collection brush-like swab A simple membrane assay method comprising a step of collecting a specimen using as an instrument and a step of subjecting a sample prepared from the collected specimen to pressure filtration using a filtration filter including a rigid filter . It is characterized by pressure-filtering the sample prepared from the specimen by applying pressure to the inside of the filtration tube that is made of a flexible material and capable of filtering the specimen prepared from the specimen. The simple membrane assay method according to claim 1. The simple membrane assay method according to claim 1 or 2, wherein a sample collected with a brush-like swab is suspended in a sample suspension to prepare a sample, and the prepared sample is filtered under pressure. The simple membrane assay method according to any one of claims 1 to 3, wherein the strong rigidity filter is a sintered filter. The simple membrane assay method according to claim 4 , wherein a material of the sintered filter is selected from the group consisting of polypropylene, polyethylene, polystyrene, and polymethylmethacrylate. Simple membrane assay method according to any one of claims 1-5 specimen is a biological sample. Pharyngeal swab specimens are human or other animal, nasal swab, nasal aspirate, claim 6 nasal wash, lavage, a specimen selected from the group consisting of stool suspension and rectal swabs Simple membrane assay method. The simple membrane assay method according to claim 7 , wherein the detected substance in the sample is a microorganism causing respiratory infection, a substance derived from the microorganism, or an antibody against them. 9. The simple substance according to claim 8 , wherein the detected substance in the sample is a pathogenic microorganism selected from the group consisting of influenza virus, RS virus, adenovirus, group A streptococcus, and pneumonia mycoplasma, a substance derived from the microorganism, or an antibody thereto. Membrane assay method. The simple membrane assay method according to claim 7 , wherein the detected substance in the specimen is a microorganism causing diarrhea, a substance derived from the microorganism, or an antibody against them. The simple membrane assay method according to claim 10 , wherein the detected substance in the sample is a pathogenic microorganism selected from the group consisting of norovirus, rotavirus, sapovirus and diarrhea adenovirus, a substance derived from the microorganism, or an antibody thereto. . The simple membrane assay method according to any one of claims 1 to 11 , which is a flow-through or lateral flow membrane assay method. The filtration filter is configured by stacking a plurality of filters having different pore diameters, and the pore diameter of the filter having the minimum pore diameter is equal to or smaller than the pore diameter of the membrane of the assay device including the membrane, and the pore diameter of the filter having the maximum pore diameter is the membrane. The simple membrane assay method according to any one of claims 1 to 12 , which is not less than 200 µm and not more than 200 µm. Specimen initially passed through a filter having a maximum pore diameter, then a simple membrane assay method of claim 13, wherein passing a filter with a smaller pore size. Simple membrane assay kit for testing the presence of, including: the object to be detected in the test body;
(1) A specimen sample filtration tube having a filtration filter constituted by a filter including a strong rigidity filter;
(2) Assay capture substance for capturing the object to be detected in the test body is provided with a bound membrane device, and
(3) Brush-like swab. The simple membrane assay kit according to claim 15, further comprising at least one of the following (4) to (7):
(4) Specimen suspension,
(5) Cleaning liquid composition,
(6) Labeling substance, and (7) Control solution. The filtration filter is configured by stacking a plurality of filters having different pore diameters, and the pore diameter of the filter having the minimum pore diameter is equal to or smaller than the pore diameter of the membrane of the assay device including the membrane, and the pore diameter of the filter having the maximum pore diameter is the membrane. The simple membrane assay kit according to claim 15 or 16 , wherein the simple membrane assay kit is not less than 200 μm and not more than 200 μm.