JP5515636B2 - Collection instrument, detection device, and collection method - Google Patents

Description

  The present invention relates to a collection instrument, a detection device, and a collection method.

  2. Description of the Related Art Conventionally, an apparatus for quickly detecting an analysis target such as bacteria in a test liquid such as saliva has been known.

JP-A-8-322594

  However, it has been difficult for the conventional apparatus to detect with sufficiently high sensitivity when the concentration of the analyte in the test solution is low.

  The present invention has been made in view of the above problems, and a collection instrument, a collection method, a detection apparatus using the same, and the like that enable rapid detection of an analysis object even when the concentration of the analysis object is low The purpose is to provide.

  The collection instrument according to the present invention includes a support plate, a porous member supported on the support plate, and a test liquid supply unit that supplies a test liquid to the porous member. The support plate has an opening at a position facing the porous member.

  According to the present invention, the test liquid is supplied from the test liquid supply section to the porous member, and the porous member is impregnated with the test liquid, and then the test liquid in the porous member is sucked through the opening. The analyte in the test solution can be concentrated on the portion of the porous member facing the opening. As a result, even if the concentration of the analyte in the test solution is low, the analyte can be concentrated on the above-mentioned part. Sensitive detection is possible quickly.

  Here, it is preferable that the portion facing the opening of the porous member has a smaller hole diameter than the portion not facing the opening of the porous member.

  Due to the difference in the pore diameter, the analysis object can be retained at a portion facing the opening of the porous member, and the analysis object can be more concentrated.

  In addition, it is also preferable that a molecular recognition element that specifically binds to the analyte in the test solution is fixed to a portion facing the opening of the porous member.

  By combining the analysis object with the molecular recognition element, the analysis object can be retained at a portion facing the opening of the porous member, and the analysis object can be more concentrated.

  Moreover, it is also preferable that the part facing the opening of the porous member is made more hydrophilic than the part not facing the opening of the porous member.

  As a result, when the test solution is aqueous, the test solution can be more easily collected in a portion facing the opening of the porous member, and the analysis target can be easily concentrated.

  Moreover, it is preferable that a test liquid supply part contains the test liquid absorber removable from a collection instrument.

  According to this, the test liquid absorber is removed from the collection instrument, the test liquid is absorbed, and then the test liquid absorber is returned to the collection instrument, so that the test liquid can be easily put into the porous member. Can supply. In addition, it is easy to collect the test liquid.

  Moreover, it is also preferable that the test liquid absorber has an annular shape surrounding the side surface of the porous member. It is also preferable that the test liquid absorber has a dish shape that surrounds the side surface of the porous member and covers the main surface of the porous member.

  According to this, it is easy to process a large amount of test liquid uniformly and quickly without unevenness.

  Moreover, it is preferable that a prefilter for collecting impurities in the test liquid is further provided between the porous member and the test liquid supply unit.

  Thereby, impurities in the test solution can be collected by the prefilter, and clogging and the like in the porous member can be suppressed.

  In addition, a labeled molecular recognition element that can be specifically bound to the analyte in the test liquid and is retained so as to be eluted with respect to the test liquid supplied to the porous member from the test liquid supply unit. It is also preferable to provide further.

  Thereby, since the analysis object is labeled, the analysis object collected in the porous member can be easily quantified.

  In addition, the collection device according to the present invention further includes a chamber chamber communicating with the opening below the support plate, the chamber chamber is provided with a suction port, and the rear-stage absorber is disposed at a position facing the opening in the chamber chamber. Is also preferably provided.

  Thereby, even if the liquid leaks through the opening, the liquid hardly leaks to the outside.

  A detection device according to the present invention comprises: suction means connected to the opening of the collection device described above; and detection means for detecting an analysis object for a portion of the collection device facing the opening of the porous member. Prepare.

  The method for collecting an analysis object according to the present invention is a method for collecting an analysis object using the above-described collection device, wherein the test solution supplied from the test solution supply unit to the porous member is opened. A suction step of sucking through.

  ADVANTAGE OF THE INVENTION According to this invention, even when the density | concentration of an analysis target object is thin, the collection instrument which enables rapid detection of an analysis target object, the collection method, a detection apparatus using this, etc. are provided.

FIG. 1 is a partially broken perspective view of a collecting device according to a first embodiment. 2 is a view taken in the direction of arrows II-II in FIG. FIG. 3 is a partially broken enlarged view of the vicinity of the porous member of FIG. FIG. 4 is a schematic perspective view of the detection apparatus according to the first embodiment. FIG. 5 is a partially broken perspective view according to a modification of the collection device according to the first embodiment. 6 is a view taken in the direction of arrows VI-VI in FIG. FIG. 7 is a cross-sectional view of the collecting device according to the second embodiment. FIG. 8 is a partially broken perspective view of the collecting device according to the third embodiment. 9 is a view taken along arrow VII-VII in FIG. FIG. 10 is the same cross-sectional view as FIG. 9 according to a modification of the collection device according to the third embodiment.

  DESCRIPTION OF EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the drawings. In the description of the drawings, the same or equivalent elements will be denoted by the same reference numerals, and redundant description will be omitted.

(Collecting instrument according to the first embodiment)
FIG. 1 is a partially broken perspective view of a collecting device according to a first embodiment. FIG. 2 is a II-II cross-sectional view of the collection device of FIG. FIG. 3 is a partially broken enlarged view in the vicinity of the opening 12a of the porous member 23 of FIG.

  The collection device 100 mainly includes a housing 10 including a support plate 12, a porous member 23, a label holder 22, a prefilter 21, and a test liquid absorber (test liquid supply unit) 20.

(Casing)
The housing 10 is made of a transparent resin and has a box shape. The housing 10 is divided into two rooms up and down by a support plate 12 arranged horizontally. Here, the upper room is the filter chamber 10a, and the lower room is the chamber chamber 10b.

(Filter room)
In the filter chamber 10a, a porous member 23, a label holding body 22, a prefilter 21, and a test liquid absorber 20 are arranged in this order on a support plate 12. An opening 12 a that communicates the filter chamber 10 a and the chamber chamber 10 b is formed in a portion of the support plate 12 that faces the porous member 23. Although the diameter of the opening 12a is not specifically limited, For example, it is about 1-15 mm, More preferably, it is 3-10 mm. The shape is not particularly limited, and examples thereof include a circle, an ellipse, a rectangle, and a square.

  An opening 10c is formed at one upper end of the filter chamber 10a. In the filter chamber 10a of the housing 10, the prefilter 21, the label holding body 22, and the porous member 23 are covered with the housing 10. . Thereby, when the gas in the filter chamber 10a is sucked from the opening 10c, the gas is sucked into the filter chamber 10a from the opening 10c, and the test liquid is passed through the prefilter 21, the label holding body 22, and the porous member 23. It is easy to extrude in order.

(Test Liquid Absorber (Test Liquid Supply Unit) 20)
The test liquid absorber 20 is an absorber that can absorb a test liquid such as saliva and can supply the absorbed test liquid to the porous member 23. The test liquid absorber 20 is not particularly limited, and examples thereof include a porous material that easily absorbs and releases a liquid sample and has a sheet form, such as sponge, filter paper, nonwoven fabric, and mesh. As the material, cotton, hemp, cellulose, nitrocellulose, cellulose acetate, rock wool, glass fiber, silica fiber, carbon fiber, boron fiber, polyamide, aramid, polyvinyl alcohol, polyvinyl acetate, rayon and other regenerated cellulose, polyester, Examples include polyurethane, nylon, polyacrylic acid, polyacrylic ester, polyethersulfone, polysulfone, polytetrafluoroethylene, polyvinylidene difluoride, and polyolefin. The pore diameter of the test liquid absorber 20 is preferably 10 to 1000 μm, more preferably 20 to 500 μm. Although the shape of these test liquid absorbers is not specifically limited, In this embodiment, it is a rectangular parallelepiped shape. Further, only one type of test solution absorber 20 can be used, but several types can be used in combination, and further, the test solution absorber 20 can be used by adhering to a non-absorber.

  In the filter chamber 10a of the housing 10, the portion above the test solution absorber 20 is the opening 10c as described above, and the test solution absorber 20 can be taken out and reinserted from the filter chamber 10a. Yes. Thereby, after removing the test liquid absorber 20 from the filter chamber 10a, the test liquid absorber 20 can be placed in the mouth and the like to absorb the test liquid such as saliva and collected and then rearranged in the filter chamber 10a. .

  The test fluid may be any fluid that contains the analyte, such as various body fluids of animals (for example, saliva, gastric fluid, bile, pancreatic juice, intestinal fluid, sweat, tears, runny nose, urine, semen, vaginal fluid, (Amniotic fluid, milk, blood, lymph, tissue fluid, body cavity fluid, cerebrospinal fluid, joint fluid, aqueous humor, intercellular fluid), plant phloem fluid or duct fluid, animal and plant cell extracts, and animal and plant cell culture Purification, food extract, fermented microorganism product, tissue extract, cell extract, microorganism culture solution, seawater, lake water, river water, rainwater, tap water, well water, agricultural water, industrial water, domestic wastewater, industrial wastewater, etc. Any solution can be used as a test solution.

  Analytes are not particularly limited, but natural compounds, synthetic compounds, saccharides, proteins, antibodies, antigens, hormones, peptides, glycoproteins, nucleic acids, saccharides, vitamins, natural compounds, synthetic compounds, cells, cell tissues, viruses, Examples thereof include a dye, a fluorescent molecule, a metal, and a metal ion.

  Specifically, for example, when saliva is used as a test solution, the analysis target is Streptococcus mutans bacteria (Sm bacteria), Streptococcus sobrinus bacteria (Ss bacteria), and Lactobacillus acidophilus that are causative agents for caries. It can be a bacterium selected from the group consisting of Rius bacterium (La bacterium), and the possibility of dental caries can be determined by measuring the amount of caries-causing bacteria in saliva.

  One end of a cover 11 formed of a flexible member is fixed to the edge of the opening 10c of the housing 10, and the opening 10c can be covered with the cover 11 as necessary. The cover 11 has a small hole (not shown) through which air can flow so that air can be supplied into the filter chamber 10a during suction. An adhesive that adheres to the housing 10 may be attached to the cover 11, and the opening 10c may be sealed.

(Prefilter 21)
The prefilter 21 is provided adjacent to the test liquid absorber 20 and collects impurities in the test liquid supplied from the test liquid absorber 20. The material for the prefilter is not particularly limited as long as it is a porous material such as a woven fabric, a nonwoven fabric, a mesh, or a membrane filter. The pore diameter is not particularly limited, but is preferably about 10 to 100 μm. Examples of the material include glass fiber, silica fiber, carbon fiber, boron fiber, cotton, hemp, cotton nitrocellulose, cellulose, cellulose mixed ester, cellulose acetate, and polycarbonate.

(Label holder 22)
The label holding body 22 is provided between the porous member 23 and the prefilter 21 and is in contact therewith.

  The label holding body 22 is an absorber that elutes the labeled molecule recognition element with respect to the test solution supplied from the test solution absorber 20 to the porous member 23. The labeled molecular recognition element is a labeled molecular recognition element that can specifically bind to an analyte and is labeled.

  Molecular recognition elements include antigens, antibodies (monoclonal antibodies, polyclonal antibodies, etc.), nucleic acid sequence fragments, effector molecules, receptor molecules, enzymes and their inhibitors, coenzymes, avidin, biotin, sugar chain compounds, lectins, aptamers (DNA aptamers) , RNA aptamers, peptide aptamers, etc.), molecular templates, and the like.

  Labels include colloidal gold, silver colloidal particles, platinum colloidal and other metal colloids, colored lipid vesicles (ribosomes) and vesicles, latex particles that may be colored, various fine particles such as magnetic fine particles, fluorescent substances, and coloring. Substances, luminescent substances, enzymes, biotin, radioisotopes, redox substances and the like can be mentioned.

  The method for holding the labeled molecule recognition element is not particularly limited, and examples thereof include an impregnation method, a coating method, and a spray method.

(Porous member 23)
The porous member 23 absorbs the test liquid supplied from the test liquid absorber 20. Examples of the porous member 23 include a woven fabric, a nonwoven fabric, a mesh, and a membrane filter. The material and pore diameter of the porous member 23 can be appropriately selected according to the analysis object. For example, when bacteria are used as the analysis target, the pore diameter is preferably 0.2 to 1 μm.

  The material is not particularly limited, and cotton, hemp, cellulose, cotton nitrocellulose, nitrocellulose, cellulose acetate, rock wool, glass fiber, silica fiber, carbon fiber, boron fiber, polyamide, aramid, polyvinyl alcohol, polyvinyl acetate, rayon, etc. Examples thereof include regenerated cellulose, cellulose mixed ester, polyester, polyurethane, nylon, polyacrylic acid, polyacrylic ester, polyethersulfone, polysulfone, polytetrafluoroethylene, polyvinylidene difluoride, polyolefin, and polycarbonate.

  As shown in FIG. 3, the porous member 23 has a portion 23a that faces the opening 12a and a portion 23b that does not face the opening 12a. At least the hole diameter of the portion 23b that does not face the opening 12a is a hole diameter through which the analyte can move.

  Here, it is preferable to make the hole diameter of the portion 23a facing the opening 12a of the porous member 23 smaller than the hole diameter of the portion 23b not facing the opening 12a of the porous member 23. By providing such a difference in the hole diameters, the analyte can be collected in the portion 23a facing the opening of the porous member, and the concentration can be very easily concentrated. In this case, when the analysis object is bacteria, the pore diameter of the portion 23a facing the opening of the porous member is preferably 0.2 to 10 μm, more preferably 0.2 to 1 μm. It is preferable that the hole diameter of the part 23b which does not oppose this opening shall be 10-200 micrometers, More preferably, it is 20-100 micrometers.

  In addition, it is also preferable to fix a molecular recognition element that specifically binds to the analysis target to the portion 23a facing the opening of the porous member. The molecular recognition element is not particularly limited, and those described above can be used. Also by using such a molecular recognition element, selective collection of the analyte is facilitated. In this case, the hole diameter of the portion 23a facing the opening of the porous member is not particularly limited. As a method for fixing the molecular recognition element, a known method can be used.

  Furthermore, it is also preferable that the material of the portion 23a facing the opening of the porous member is a material having higher hydrophilicity than the portion 23b not facing the opening of the porous member. Specifically, for example, a material having a relatively low hydrophilicity such as glass fiber, silica fiber, carbon fiber, or boron fiber is used as the material of the portion 23b that does not face the opening of the porous member, and the material faces the opening of the porous member. Examples of the material of the portion 23a include using a relatively hydrophilic material such as cotton, hemp, cotton nitrocellulose, cellulose, cellulose mixed ester, cellulose acetate, and polycarbonate.

  Further, the surface of the portion 23b not facing the opening of the porous member is treated with a hydrophobic surface treatment agent such as fluororesin or silicone resin, and the surface of the portion 23a facing the opening of the porous member is treated with a hydrophilic surface treatment such as It is also possible to treat with an agent and to perform both of these. Such a surface treatment can be performed by UV irradiation, corona discharge, plasma treatment or the like without using a surface treatment agent. Thus, when the test solution is aqueous, the test solution can be easily collected in the portion 23a facing the opening of the porous member, and the analysis target can be easily concentrated. In particular, when the portion 23b that does not oppose the opening of the porous member is subjected to water repellent treatment, it is possible to prevent the analysis target from being collected and adsorbed on an extra portion. In addition, the development of the test solution becomes rapid.

  The size of the porous member 23 is not particularly limited, but is preferably sufficiently larger than the opening 12a. The area is preferably 1.1 to 50.0 times, more preferably 2 to 25 times the area of the opening 12a.

(Chamber room)
A rear-stage absorber 31 is provided in the chamber chamber 10b at a position facing the opening 12a. The post-stage absorber 31 collects a liquid that may leak from the opening 12a, and the same material as the test liquid absorber 20 can be used as the material.

  A suction tube 8 is provided on a side surface of the chamber chamber 10b so that the chamber chamber 10b communicates with the outside.

(measuring device)
As shown in FIG. 4, the measurement apparatus 400 according to the present embodiment is a measurement apparatus that detects an analysis object using the above-described collection tool 100. This measuring device 400 is connected to the suction pipe 8 of the collection instrument 100 and is a pump 401 as a suction means for sucking gas from the inside of the housing 10, and a part of the collection instrument 100 facing the opening 12 a of the porous member 23. And a detection unit 403 that detects an analysis object with respect to 23a.

  The configuration of the detection unit 403 is not particularly limited. For example, when an analysis object is optically detected, the portion 23a facing the opening 12a of the porous member 23 is irradiated with predetermined light, and the reflected light or fluorescence of the analysis object or the label bound to the analysis object is irradiated. And the like, the presence / absence and concentration of the analysis object can be calculated.

  Further, when electrochemically detecting an analyte, a measurement electrode is brought into contact with the portion 23a facing the opening 12a of the porous member 23, and an electrical response derived from the analyte or a label bound to the analyte is measured. The presence / absence and concentration of the analysis object can be calculated.

  Next, the operation of this embodiment will be described.

  First, the test liquid absorber 20 of the collection device 100 of FIG. 1 is taken out from the filter chamber 10a through the opening 10c of the housing 10 and, for example, contained in the mouth, the test liquid such as saliva can be tested. The test solution absorber 20 is sufficiently absorbed. Subsequently, the test liquid absorber 20 in which the test liquid is absorbed is returned into the filter chamber 10 a through the opening 10 c and brought into contact with the prefilter 21. And the cover 11 is closed as needed.

  Subsequently, as shown in FIG. 4, the suction tube 8 of the collection instrument 100 is connected to the pump 401 of the measuring device 400 to start suction. As a result, the gas in the housing 10 is discharged from the suction pipe 8, and the air flowing in from the opening 10 c pushes out the test liquid in the test liquid absorber 20, so that the test liquid is retained in the prefilter 21 and the label holding. It passes through the body 22 and reaches the porous member 23. At this time, passing through the pre-filter 21 traps impurities such as food waste in the test liquid. Furthermore, by passing through the label holding body 22, the labeled molecule recognition element is eluted in the test solution, and this labeled molecule recognition element is used for the bacteria (Streptococcus mutans bacteria or Lactobacillus acidophila in the test solution pair. And the like, and the analyte is labeled.

  Then, as shown in FIG. 3, the test solution that has reached the porous member 23 flows toward the portion 23a facing the opening 12a by suction, and the portion 23a facing the opening of the porous member as compared with the case where the suction is not performed. The concentration of the analysis object 56 is higher than that of the portion 23b that does not face the opening of the porous member, and the concentration of the analysis object can be increased. In particular, by analyzing the pore diameter, fixing of the molecular recognition element, surface treatment, etc., the portion 23a of the porous member 23 that faces the opening 12a is easier to collect than the portion 23b of the porous member 23 that does not face the opening 12a. If it is set as a structure, an analysis object can be concentrated very efficiently in the part 23a which opposes the opening 12a of the porous member 23, and its vicinity.

  Even if the structure of the portion 23a of the porous member 23 that faces the opening 12a and the portion 23b of the porous member 23 that does not face the opening 12a are the same, some of the analyte flows out through the opening 12a. Although there is a possibility, the analysis object can be concentrated in a narrow region as compared with the case where the opening 12a is not provided. In this case, the suction may be stopped at an appropriate time before the entire analysis target is discharged.

  A part of the liquid passes through the porous member 23 and reaches the chamber chamber 10 b through the opening 12 a, but this liquid is trapped in the rear-stage absorber 31. The suction by the pump is appropriately stopped when the concentration of the analyte in the portion 23a facing the opening 12a of the porous member 23 is completed.

  Subsequently, the detection unit 403 performs a desired detection process on the portion 23a facing the opening 12a of the porous member 23, and measures the presence / absence of the analysis object and its concentration.

  According to this embodiment, the opening 12 a is formed in the support plate 12 at a position facing the porous member 23. For this reason, by aspirating the lower side of the opening 12a as a negative pressure, the analysis object 56 in the test liquid can be concentrated on a portion of the porous member 23 facing the opening 12a. Thereby, since the density | concentration of the analysis target object in the porous member 23 can be made high about a specific area | region, even if it is a case where a test liquid contains the analysis target object 56 in a low density | concentration, it is highly sensitive and culture | cultivates It becomes possible to detect quickly without trouble.

  Then, with reference to FIG.5 and FIG.6, the deformation | transformation aspect of the collection instrument 100 which concerns on 1st embodiment is demonstrated. In this aspect, there is no opening 10c on the upper surface of the housing 10, and the opening 10c is provided on the side surface (side surface on the Y direction side) of the housing 10, and the edge of the opening 10c is covered so as to cover the opening 10c. 11 is provided. In this embodiment, it is the same as that of the above-mentioned aspect except inserting the test liquid absorber 20 along the Y direction from the opening 10c. This aspect also has the same effect as described above. The opening 10c may be provided not on the side surface on the Y direction side but on the side surface on the X direction side.

(Collecting instrument 200 according to the second embodiment)
Then, the collection instrument 200 which concerns on 2nd embodiment with reference to FIG. 7 is demonstrated. The collecting instrument 200 according to the present embodiment is different from the collecting instrument 100 according to the first embodiment in that the support plate 12 also serves as the bottom surface of the housing 10, that is, there is no chamber chamber 10b, and the filter chamber. The suction pipe 8 is directly connected to the opening 12a. Also in this embodiment, there exists an effect similar to 1st Embodiment. In some cases, liquid may be sucked into the pump 401 of the detection device 400. In this case, a liquid trap may be provided on the pump side. Thereby, size reduction of the collection instrument 200 can be achieved.

(Catching instrument 300 according to the third embodiment)
Next, the collection device 300 according to the third embodiment will be described with reference to FIGS. 8 and 9. The collection device 300 includes a bottomed cylindrical casing 10 having an open top, and the porous member 23 has a disk shape. The label holder 22, the prefilter 21, and the test object The liquid absorber 20 has an annular shape that sequentially surrounds the porous member 23. Other than these, the second embodiment is the same as the first embodiment.

  Also according to the present embodiment, the same effects as those of the first embodiment can be obtained. In the third embodiment, since the upper part of the housing 10 is opened, the movement of the test liquid from the test liquid absorber 20 to the porous member 23 is mainly based on the capillary phenomenon, After the test solution has sufficiently penetrated into the member 23, the analyte in the test solution can be concentrated on the portion 23a of the porous member 23 facing the opening 12a by suction through the opening 12a. .

  Then, with reference to FIG. 10, the deformation | transformation aspect of the collection instrument 300 which concerns on 3rd embodiment is demonstrated. In this embodiment, the label holder 22, the prefilter 21, and the test liquid absorber 20 are dish-shaped so as to cover the upper surface of the porous member 23, respectively. In this embodiment, the same operation as described above is achieved. A larger amount of test liquid can be processed.

  The present invention is not limited to the above embodiment, and various modifications can be made.

  For example, in the above-described embodiment, the removable test liquid absorber 20 is used as the test liquid supply unit that supplies the test liquid to the porous member 23, but the non-removable test liquid absorber is not used. 20 may be provided. In this case, the test liquid may be dropped onto the test liquid absorber 20. In addition, a recess that accepts a liquid supplied from the outside without providing an absorber, such as a recess that communicates with the porous member 23 via the marker holder 22 and the prefilter 21 without providing the test solution absorber 20. It may be said. In this case as well, the test solution may be dropped into the recess.

  In the above embodiment, the test liquid supply section (for example, the test liquid absorber 20) and the porous member 23 are directly connected to each other by the marker holder 22 and the prefilter 21, so Although it is possible to supply the test solution, there is no direct contact between the test solution supply unit and the prefilter 21, between the prefilter 21 and the label holding body 22, and between the label holding body 22 and the porous member 23. Even if they are connected via a flow path, they can be implemented. The test solution can be moved by suction.

  Moreover, in the said embodiment, although the labeled molecule recognition element is hold | maintained so that elution is possible with respect to the label | marker holding body 22, it elutes with respect to the test liquid supplied to a porous member from the test liquid supply part 2. FIG. What is necessary is just to fix to the place which can be, and it may apply | coat etc. to a flow path, a support plate, a test liquid absorber. Of course, implementation is possible without a labeled molecule recognition element.

  Further, the present invention can be implemented without the label holder 22 and the prefilter 21. Also in that case, it is preferable that the test liquid supply part and the porous member 23 are directly connected.

  Moreover, in 1st embodiment, except opening 10c is sealed, an open type may be sufficient.

  Moreover, the housing | casing 10 should just make the part 23a which opposes the opening 12a in the porous member 23 light-transmitting, and the part 23a which opposes the opening 12a may be made into the opening.

  Furthermore, the shapes of the housing 10, the suction tube 8, and various absorbers are not limited to the above embodiment.

  A collecting device 100 shown in FIG. 1 was manufactured. The case 10 was made of PET resin and had a size of 16 cm × 5 cm × 5 cm. The diameter of the opening 12a was 8 mm.

  By using a nonwoven fabric made of glass fiber having a pore diameter of 1 μm as the portion 23 b not facing the opening 12 a of the porous member 23 and using nitrocellulose having a pore diameter of 0.45 μm for the portion 23 a facing the opening 12 a of the porous member 23, The portion 23a facing the opening of the member made it easier to collect bacteria than the portion 23b not facing the opening of the porous member.

  A glass fiber non-woven fabric was used as the label holding body 22, and this label holding body 22 was impregnated with Sm bacteria-binding aptamer labeled with gold colloid particles so as to be eluted.

  As the prefilter 21, a glass fiber filter having a pore diameter of 10 μm was used.

  Cellulose sponge was used as the test liquid absorber 20.

  A PET film was used for the cover.

  After holding the test liquid absorber 20 in the subject's mouth and absorbing saliva, it is returned to the filter chamber 10a, covered with a cover, the suction tube 8 is connected to the pump 401 of the test apparatus 400, and negative pressure is applied. I made it.

  As a result, saliva passes through the prefilter 21 to remove dust such as epidermal cells and food waste mixed in the saliva, and subsequently passes through the label holding body 22 so that the labeled aptamer is in saliva. Then, the Sm bacteria that dissolved into the Sm bacteria and bound to the Sm bacteria were trapped in the portion 23a facing the opening of the porous member. This portion was colored red by colloidal gold particles bound to trapped Sm bacteria. The green LED light provided in the detection means of the measuring device was irradiated on this portion, the reflected light intensity was read with a photodiode, and the Sm bacteria concentration could be calculated.

  DESCRIPTION OF SYMBOLS 10 ... Housing | casing, 10a ... Filter chamber, 10b ... Chamber chamber, 12 ... Support plate, 12a ... Opening, 20 ... Test liquid absorber (test liquid supply part), 21 ... Pre filter, 23 ... Porous member, 23a ... parts facing the opening of the porous member, 23b ... parts not facing the opening of the porous member, 31 ... second-stage absorber, 100, 200, 300 ... collecting device, 400 ... detecting device, 401 ... pump (suction means), 402: Detection means.

Claims (11)

A support plate;
A porous member supported on the support plate;
A test liquid absorber for supplying a test liquid to the porous member, and
The support plate is formed with a suction opening at a position facing the porous member,
The said test liquid absorber is a collection instrument which makes the annular | circular shape which surrounds the side surface of the said porous member.   The collection instrument according to claim 1, wherein a portion of the porous member that faces the opening has a smaller hole diameter than a portion of the porous member that does not face the opening.   The collection device according to claim 1 or 2, wherein a molecular recognition element that specifically binds to an analyte in the test solution is fixed to a portion of the porous member facing the opening.   The collection instrument according to any one of claims 1 to 3, wherein a portion of the porous member that faces the opening is made more hydrophilic than a portion of the porous member that does not face the opening.   The said test liquid absorber is detachable from the said collection instrument, The collection instrument of any one of Claims 1-4.   The said test liquid absorber is a collection instrument as described in any one of Claims 1-5 which makes | forms the dish shape which covers the main surface of the said porous member while surrounding the side surface of the said porous member.   The collection instrument according to any one of claims 1 to 6, further comprising a prefilter that collects contaminants in the test liquid between the porous member and the test liquid absorber.   A labeled molecule that can be specifically bound to the analyte in the test solution and is labeled so as to be eluted from the test solution supplied from the test solution absorber to the porous member. The collection instrument according to any one of claims 1 to 7, further comprising a recognition element. A chamber chamber communicating with the opening is further provided under the support plate,
The collection device according to claim 1, wherein a suction port is provided in the chamber chamber, and a rear-stage absorber is provided in the chamber chamber at a position facing the opening.   The suction means connected to the opening of the collection device according to any one of claims 1 to 9, and the detection of the analysis object for the portion of the collection device facing the opening of the porous member. Detecting means for performing.   A method for collecting an analysis object using the collection device according to claim 1, wherein the test liquid supplied from the test liquid absorber to the porous member is passed through the opening. The collection method provided with the suction process sucked through.

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