JP2008049311A - Microchip plate - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microchip plate which is formed by joining a first base stock to a second base stock by using an adhesive sheet and has a proper channel for preventing the adhesive sheet from intruding into channels to be formed in the base stocks. <P>SOLUTION: The microchip plate 1 is formed by joining the first base stock to the second base stock by using the adhesive sheet 6 interposed between the first base stock 2 and the second base stock 3. When the first base stock 2 and the second base stock 3 are pressed to the adhesive sheet 6 and joined to each other, an intrusion preventing means 7 for preventing the adhesive sheet 6 from intruding into the channels 4 to be formed on the surfaces of both base stocks 2, 3 to be opposed to each other is formed on both of the first base stock 2 and the second base stock 3. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a microchip plate, and more particularly to a microchip plate suitable for performing various chemical processes on a chip.

  With recent progress in microchemical technology, it is frequently used on the chip for chemical reactions including complex formation reaction, organic synthesis, solvent extraction, cell culture, chemical reaction control and the like. In particular, it is called a biochip, and a microchip plate is used for DNA identification and the like.

  Various manufacturing methods and the like have been proposed for this type of microchip plate (see Patent Document 1).

  FIG. 2 shows an example of a conventional microchip plate.

  This microchip plate 21 uses a thin adhesive sheet 26 for a second material 23 in which a channel 24 and a hole 25 are formed in a minute flow path with respect to a first flat plate material 22 made of glass. Are formed by bonding. As a material of the adhesive sheet 26, one kind of silicone resin such as PDMS (polydimethylsiloxane) is often used.

  When DNA is detected on the microchip plate 21, a liquid specimen containing DNA is put into the channel 24 through the hole 25, and the DNA is linked in the channel 24 with the detection DNA having a fluorescent substance. Whether or not it is detected by irradiating light through the first material 22 and the second material 23 is detected.

JP 2005-111567 A

  However, in the conventional microchip plate 21, as shown in FIG. 3, when the adhesive sheet 26 is sandwiched between the first material 22 and the second material 23 and both are adhered, In some cases, the adhesive sheet 26 bites into the channel 24 serving as a path. This is because the adhesive sheet 26 is pressed between the first material 22 and the second material 23 in a state where the adhesive sheet 26 is heated and elastically deformed. It is generated because it is deformed, bites into the channel 24 and hardens as it is.

  When the adhesive sheet 26 is deformed and bites into the channel 24 in this way, the cross-sectional area and volume of the channel 24 become small, and it becomes difficult for the liquid and the substance in the channel to flow. There was an inconvenience that this reaction was not performed properly.

  Further, today, there is a demand for further miniaturization of the cross-sectional area of the channel 24, and the smaller the cross-sectional area, the higher the need to prevent the adhesive sheet from biting into the channel 24. is there.

  The present invention has been made in view of these points. In a microchip plate formed by bonding a first material and a second material using an adhesive sheet, the adhesive sheet is formed on the material. It is an object of the present invention to provide a microchip plate having an appropriate channel that does not bite into the channel.

  In order to achieve the above object, a microchip plate according to the present invention is a microchip plate formed by bonding using an adhesive sheet having a first material and a second material interposed therebetween, Intrusion prevention that prevents the adhesive sheet from biting into a channel formed on one opposing surface of the two materials when the adhesive sheet is pressed and joined by the first material and the second material. The means is formed in the first material and the second material.

  According to the microchip plate of the present invention, when the first material and the second material are bonded to each other by interposing an adhesive sheet therebetween, the biting prevention means has the adhesive sheet of the both materials. It is possible to prevent the adhesive chip from biting into the channel formed on the opposite surface, and to obtain a microchip plate having an appropriate channel that does not bite into the channel.

  Further, in the microchip plate according to the present invention, the biting prevention means is an uneven portion formed in the vicinity of the channel on the opposing surface of the two materials, and is engaged with the adhesive sheet interposed therebetween. The slackness of the adhesive sheet is prevented.

  According to the microchip plate of the present invention, the concave and convex portions formed in the vicinity of the channel on the opposing surfaces of both materials are engaged with each other with the adhesive sheet interposed therebetween, and the adhesive sheet is prevented from sagging in the channel. In addition, the adhesive sheet is reliably prevented from biting into the channel.

  In the microchip plate according to the present invention, the uneven portion is formed at a position along the channel of the material on which the channel is formed.

  According to the microchip plate of the present invention, since the uneven portion is formed along the channel, the adhesive sheet is engaged over the entire length of the channel by engaging the adhesive sheet with the uneven portion on both sides of the channel. Can be reliably prevented from entering the channel.

  In the microchip plate according to the present invention, the adhesive sheet is formed of PDMS, polyolefin, or a mixture thereof.

  According to the microchip plate of the present invention, since the adhesive sheet is PDMS, polyolefin, or a mixture thereof, when the pressure is sandwiched between the first material and the second material and pressed, the channel is prevented by the biting prevention means. It is very well prevented from being pulled into the channel by being pulled in both directions in the width direction.

  Further, in the microchip plate according to the present invention, the material in which the channel is formed is characterized in that the channel and the biting prevention means are formed by integral molding of resin with polyolefin.

  According to the microchip plate of the present invention, the formation of the channel and the biting prevention means can be simplified, it is possible to cope with the miniaturization of the cross-sectional area of the channel, and the bonding with the adhesive sheet is also strong.

  As described above, according to the microchip plate of the present invention, in the microchip plate formed by bonding the first material and the second material using the adhesive sheet, the adhesive sheet is the material. The microchip plate having an appropriate channel that does not bite into the formed channel can be obtained.

  Hereinafter, an embodiment of a microchip plate according to the present invention will be described with reference to FIG.

  FIG. 1 shows one embodiment of the microchip plate of the present invention, where FIG. 1 (a) shows a state before joining, and FIG. 1 (b) shows a state after joining.

  In the microchip plate 1 of the present embodiment, a channel 4 or a hole (not shown) in the form of a micro flow channel is formed on the first flat plate-like material 2 made of glass or resin, as in the past. The second material 3 is bonded by using a thin adhesive sheet 6.

  In the present invention, when the adhesive sheet 6 is further pressed and joined to the structure by the first material 2 and the second material 3, the adhesive sheet 6 is placed on one opposing surface of the both materials 1 and 2. The first material 2 and the second material 4 are formed with a biting prevention means 7 for preventing biting into the formed channel 4. In this embodiment, the biting prevention means 7 is formed so as to prevent the adhesive sheet 6 from sagging in the channel 4. Specifically, as shown in FIG. 1, the biting prevention means 7 is a concavo-convex portion 8 formed in the vicinity of the channel 4 on the opposing surface of both materials 2 and 3, and sandwiches the adhesive sheet 6 from each other. By engaging and pulling the adhesive sheet 6 in the width direction of the channel 4, it is prevented from sagging. In the present embodiment, one recess 8 a that forms the concavo-convex portion 8 is formed in the thick second material 4 because the channel 4 is formed. The other convex portion 8 b that forms the concave-convex portion 8 is formed on the flat first material 3. The concave-convex portion 8 as the biting prevention means 7 is located along the channel 4 of the second material 3 where the channel 4 is formed, specifically along the longitudinal direction of the channel 4 at both positions in the width direction of the channel 4. Is formed.

  The depth and width of the channel 4 formed in the second material 3 are each about 50 μm, and the thickness of the second material 3 is about twice the depth of the recess 8a. When the raw material is a resin such as polyolefin, the second material 3 is formed by integral molding of the resin so as to provide the channel 4, the recess 8a, and a port (not shown). If low fluorescence is not necessary for the properties of the resin in the inspection, the second material 3 may be formed of an acrylic resin.

  The thickness of the first material 2 is about 20 μm, and the height and width of the convex portion 8b formed in the first material 2 are inserted into the concave portion 8a together with the adhesive sheet 6 and engaged. It is formed in an appropriate size. When the raw material is, for example, a resin such as polyolefin, the first material 2 is formed by integral molding of the resin so as to provide the convex portion 8b. When low fluorescence is not necessary for the property of the resin in the inspection, the first material 2 may be formed of an acrylic resin.

  The thickness of the adhesive sheet 6 before being pressed by the two materials 1 and 2 is about 20 μm. As the material, one kind of silicone resin such as PDMS (polydimethylsiloxane) is used. Specifically, PDMS alone, polyolefin resin alone, or a mixture of PDMS and polyolefin in a mixing ratio (molar ratio) of 1: 9 to 5: 5 is preferable. If both the raw materials 2 and 3 and the adhesive sheet 6 are made of a resin material, the mutual bonding is firmly performed, and the microchip plate 1 becomes higher quality.

  Next, a method for manufacturing the microchip plate 1 of the present embodiment will be described.

  As shown in FIG. 1A, the first material 2 in which one convex portion 8 b of the concave and convex portion 8 as the biting prevention means 7 is formed on the opposing surface side, and the other concave portion 8 a is provided on the opposing surface along the channel 4. The adhesive sheet 6 is interposed between the second material 3 formed on the side.

  Subsequently, both the raw materials 2, 3 and the adhesive sheet 6 are heated by a hot press (not shown) to heat the adhesive sheet 6 to a state where it can be elastically deformed and gradually compressed. As a result, each convex portion 8b is inserted together with the adhesive sheet 6 into each concave portion 8a, and both side portions in the width direction of the channel 4 of the adhesive sheet 6 facing each channel 4 are drawn into the concave portion 8a. Pull like so. The adhesive sheet 6 that has received this tensile force is elastically deformed so as to extend in both sides of the channel 4 in the width direction, and is reliably prevented from getting into the channel 4.

  Subsequently, by curing the adhesive sheet 6, both the raw materials 2 and 3 are joined by the adhesive sheet 6 as shown in FIG. 1B, and the microchip plate 1 is completed.

  According to the microchip plate 1 of the present embodiment, when the first material 2 and the second material 3 are bonded to each other with the adhesive sheet 6 interposed therebetween, the biting prevention means 7 is used as an adhesive. It is possible to reliably prevent the sheet 6 from biting into the channel 4 formed on one facing surface of the two materials 2 and 3.

  Further, by providing the concavo-convex portion 8 as the biting prevention means 7, the concavo-convex portion 8 formed in the vicinity of the channel 4 on the opposite surface of the two materials 2 and 3 is engaged with and bonded to each other with the adhesive sheet 6 interposed therebetween. It is possible to prevent the sheet 6 from sagging in the channel 4.

  Further, since the uneven portion 8 is formed along the channel 4, the adhesive sheet 6 is engaged with the uneven portion 8 on both sides of the channel 4 so that the adhesive sheet 6 extends over the entire length of the channel 4. Intrusion into the channel 4 can be reliably prevented.

  Further, since the adhesive sheet 6 is PDMS, polyolefin, or a mixture thereof, both sides of the channel 6 in the width direction by the biting prevention means 7 when being pressed between the first material 2 and the second material 3 are pressed. It is very well prevented that it is pulled in the direction and bites into the channel 4. Furthermore, the channel 4 and the biting prevention means 7 can be easily formed by integral molding of the resin, and even if the cross-sectional area of the channel 4 becomes fine, it can be easily formed to cope with the miniaturization. it can.

  In addition, this invention is not limited to the said embodiment, A various change is possible as needed.

  For example, the concave portion 8 a and the convex portion 8 b of the concavo-convex portion 8 may be installed reversely with the first material 2 and the second material 3. Furthermore, the concavo-convex portion 8 may be used as a positioning means for both the raw materials 2 and 3 or may be used as a caulking coupling means. In the case of caulking and coupling means, the uneven portion 8 is preferably a male-female engaging member having a retaining action.

  Further, as a biting prevention means, the opposing surfaces of the first material 2 and the second material 3 other than the channel 4 are not engaged with each other as in the above embodiment, but are stuck into the adhesive sheet 6 and exert grip force. And you may make it form many one or both of the micro convex part which prevents the adhesive agent sheet 6 from deform | transforming into the channel 4, and a recessed part.

It is sectional drawing which shows embodiment of the microchip plate which concerns on this invention, (a) is sectional drawing which shows the state before joining, (b) is sectional drawing which shows after joining A perspective view showing a conventional microchip plate FIG. 3 is an enlarged cross-sectional view taken along line 3-3.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Microchip plate 2 1st material 3 2nd material 4 Channel 6 Adhesive sheet 7 Biting-in prevention means 8 Concave portion 8a Concavity 8b Convex

Claims (5)

  A microchip plate formed by bonding using an adhesive sheet having a first material and a second material interposed therebetween, wherein the adhesive sheet is compressed by the first material and the second material The first material and the second material are formed with a biting prevention means for preventing the adhesive sheet from biting into a channel formed on one opposing surface of the two materials when being joined together. A featured microchip plate.   The biting prevention means is a concavo-convex portion formed in the vicinity of the channel on the opposing surfaces of the two materials, and is engaged with each other with the adhesive sheet interposed therebetween to prevent sagging of the adhesive sheet. The microchip plate according to claim 1.   The microchip plate according to claim 2, wherein the uneven portion is formed at a position along the channel of the material on which the channel is formed.   The microchip plate according to any one of claims 1 to 3, wherein the adhesive sheet is formed of PDMS, polyolefin, or a mixture thereof. The micro material according to any one of claims 1 to 4, wherein the material in which the channel is formed is formed by integrally molding the channel and the biting prevention means with polyolefin. Chip plate.

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