JP2008017782A - Growth accelerating agent for mushroom cultivation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new cultivation growth accelerating agent increasing yield in artificial cultivation of mushroom such as Pleurotus ostreatus, Flammulina velutipes and Pholiota nameko. <P>SOLUTION: This new cultivation growth accelerating agent has oligosaccharide as the active ingredient. The technology to promote growth of mushroom such as Pleurotus ostreatus, Flammulina velutipes and Pholiota nameko comprises adding the accelerating agent to a culture medium of mushroom, and especially, the content of nigerooligosaccharide and/or β-glucooligosaccharide is brought to 0.01-10 pts.wt. based on 100 pts.wt. of the additive so as to promote growth of mushroom. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a growth promoter for mushroom cultivation.

  Cultivation of edible mushrooms is broadly divided into raw wood cultivation and fungus bed (culture medium) cultivation, and in-house cultivation with artificial fungus beds that excel in cost and productivity is increasing year by year.

  In this fungus bed cultivation method, (1) Mushroom cultivation medium is prepared by adding fresh rice bran, bran, soybean hulls, okara, etc. to sawdust medium, and adding water to adjust the water content, (2) The process of sterilizing this medium and inoculating the target mushroom fungus, and (3) sufficiently culturing the mycelium and performing fungus oyster and germination operations to obtain fruit bodies (mushrooms) are adopted. Yes.

  The feature of this fungus bed cultivation is that an additive having an effect of increasing the yield is used in the medium, and the additive is basically a mixture of sawdust medium, rice bran, bran, soybean hulls, and okara.

  These additives have mushroom growth-promoting action due to the water retention and physical properties of the additives, and due to the interstitial nature of the additives, they also contain carbohydrates such as starch, proteins, fats, vitamins, etc. Contains and acts as a mushroom nutrient.

However, there is a need for better additives to improve productivity and reduce costs.
JP 63-276484 A JP-A-10-94326

  An object of this invention is to provide the new cultivation growth promoter which can increase a yield in artificial cultivation of a mushroom.

  As a result of various investigations focusing on oligosaccharides as substances for promoting the growth of mushrooms, the present inventors have found that this is effective for promoting the growth of mushrooms.

  The present invention has been made based on this finding, and is a cultivation growth promoter for mushrooms containing oligosaccharides, particularly nigerooligosaccharides and / or β-glucooligosaccharides as active ingredients.

  The present invention is a technique for promoting the growth of mushrooms by adding a growth promoter for mushroom cultivation containing an oligosaccharide as an active ingredient to a culture medium.

  The fungus bed (culture medium) holds nutrients and water-absorbing polymer substances and forms a cultivation bed. In other words, there is no particular limitation as long as it can be decomposed into nutrients, but base materials mainly composed of lignin and cellulose such as sawdust and corn cob and nutrient sources such as rice bran, cottonseed husk, bran, soybean hulls, okara etc. The containing base material etc. are illustrated and these can be used individually or in combination.

  The growth promoter for mushroom cultivation is preferably added to the culture medium so that it is dispersed throughout the culture medium. It may be added simultaneously with powders such as sawdust, rice bran, and bran, or may be added after adding water. Good. Moreover, it is possible to inject and add immediately after inoculation or in the middle of culturing as long as the hyphae spread is completed.

  The oligosaccharide of the present invention is a combination of 2 to 10 monosaccharides, and is preferably 0.01 to 10 parts by mass with respect to 100 parts by mass of the additive. Lactose, trehalose, maltose, xylooligosaccharide, galacto-oligo Examples include sugar, soybean oligosaccharide, fructooligosaccharide, dairy oligosaccharide, isomaltoligosaccharide, panose oligosaccharide, raffinose, palatinose, lactulose, β-glucooligosaccharide, and nigerooligosaccharide, and β-glucooligosaccharide and nigerooligosaccharide are particularly preferable.

  The β-glucooligosaccharide of the present invention is an oligosaccharide in which glucose is β-glucoside-bonded, and in the case of a disaccharide depending on the bonding position, sophorose, laminaribiose, cellobiose, and gentiobiose are exemplified, and the structure of these disaccharides is It is an oligosaccharide contained in one or more. Examples of the manufacturing method include the techniques of JP-A 09-299094 and JP-A 09-299095.

  The nigerooligosaccharide of the present invention means an oligosaccharide having a glucose polymerization degree of 2 or more containing at least one α-1,3 glucoside bond, such as nigerose, nigerosyl glucose, nigerosyl maltose, etc., preferably glucose Examples thereof include oligosaccharides having a degree of polymerization of 2 to 10, more preferably oligosaccharides having a degree of polymerization of 2 to 7. The manufacturing method is exemplified by the technique described in Japanese Patent No. 2721968.

  The oligosaccharide of the present invention is preferably 0.01 to 10 parts by mass with respect to 100 parts by mass of the additive, and may be in any form such as a liquid, powder or crystal. From the viewpoint of production cost, a liquid or powder is preferable to a crystal. These liquids and powders contain saccharides other than the oligosaccharides of the present invention, but saccharides other than the oligosaccharides of the present invention such as glucose may cause the growth of competing microorganisms other than mushroom mycelia, It is desirable to use as little as possible.

  EXAMPLES Hereinafter, the present invention will be specifically described with reference to examples, but the present invention is not limited to the examples.

  We purchased commercially available oyster mushroom beds and examined the effects of various carbohydrates on oyster mushroom culture.

  After immersing the oyster mushroom bed with Gentose # 80P (β-glucooligosaccharide-rich syrup: manufactured by Nippon Shokuhin Kako Co., Ltd.) in 0.01% by weight aqueous solution for 3 hours, culturing at 15 ° C. and RH 95% The situation was observed.

  The saccharide of Example 1 was powdered nigero-oligosaccharide (manufactured by Nippon Shokuhin Kako Co., Ltd.), and everything else was carried out in the same manner.

(Comparative Example 1)
The procedure was the same except that the saccharide of Example 1 was not added.

(Comparative Example 2)
The saccharide of Example 1 was glucose, and everything else was performed in the same manner.

It is a photograph which shows the state at the time of the test start in Examples 1 and 2 and Comparative Examples 1 and 2. It is a photograph which shows the state at the time of completion | finish of a test in Example 1 and 2 and Comparative Example 1 and 2. FIG.

Claims (3)

  Growth promoter for mushroom cultivation containing oligosaccharide as an active ingredient   2. The growth promoter for mushroom cultivation according to claim 1, wherein the oligosaccharide is nigerooligosaccharide and / or β-glucooligosaccharide.   The nigerooligosaccharide is one or more selected from nigerose, nigerosyl glucose, nigerosylose, and nigerosyl maltose, and the β-glucooligosaccharide is selected from gentiooligosaccharide, sophorooligosaccharide, laminarioligosaccharide, and cellooligosaccharide The growth promoter for mushroom cultivation according to claim 3, wherein the growth promoter is one or more.