JP2007523636A5 - - Google Patents
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- Publication number
- JP2007523636A5 JP2007523636A5 JP2006547528A JP2006547528A JP2007523636A5 JP 2007523636 A5 JP2007523636 A5 JP 2007523636A5 JP 2006547528 A JP2006547528 A JP 2006547528A JP 2006547528 A JP2006547528 A JP 2006547528A JP 2007523636 A5 JP2007523636 A5 JP 2007523636A5
- Authority
- JP
- Japan
- Prior art keywords
- rna
- extracted
- phenol
- tissue
- minutes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920000160 (ribonucleotides)n+m Polymers 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 210000001519 tissues Anatomy 0.000 description 2
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 108020005089 Plant RNA Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
Description
組織は、針葉樹針、木部、形成層、または師部から得られる。組織は、液体窒素で凍結して粉砕する。総RNAをコンサート植物RNA試薬(Invitrogen)を使用して抽出する。生じるRNA試料をフェノール:クロロホルム中に抽出して、DNaseで処理する。次いで、RNAを65℃で2分間インキュベートし、続いて4℃で30分間遠心分離する。遠心分離に続いて、混入物を除去するためにRNAを少なくとも10回フェノールに抽出する。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US53303603P | 2003-12-30 | 2003-12-30 | |
PCT/US2004/043804 WO2005065339A2 (en) | 2003-12-30 | 2004-12-30 | Cell cycle genes and related methods of using |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2010251599A Division JP2011097941A (ja) | 2003-12-30 | 2010-11-10 | 細胞周期遺伝子および関連した使用方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2007523636A JP2007523636A (ja) | 2007-08-23 |
JP2007523636A5 true JP2007523636A5 (ja) | 2011-01-06 |
Family
ID=34748844
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006547528A Pending JP2007523636A (ja) | 2003-12-30 | 2004-12-30 | 細胞周期遺伝子および関連した使用方法 |
JP2010251599A Pending JP2011097941A (ja) | 2003-12-30 | 2010-11-10 | 細胞周期遺伝子および関連した使用方法 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2010251599A Pending JP2011097941A (ja) | 2003-12-30 | 2010-11-10 | 細胞周期遺伝子および関連した使用方法 |
Country Status (10)
Country | Link |
---|---|
US (2) | US7598084B2 (ja) |
EP (1) | EP1711592A4 (ja) |
JP (2) | JP2007523636A (ja) |
CN (1) | CN1954071A (ja) |
AR (1) | AR047574A1 (ja) |
AU (1) | AU2004311384B2 (ja) |
BR (1) | BRPI0418229A (ja) |
NZ (1) | NZ548845A (ja) |
WO (1) | WO2005065339A2 (ja) |
ZA (1) | ZA200606198B (ja) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20110047644A1 (en) * | 1999-03-11 | 2011-02-24 | Marion Wood | Compositions and methods for the modification of gene transcription |
AR047574A1 (es) | 2003-12-30 | 2006-01-25 | Arborgen Llc 2 Genesis Res 1 | Genes del ciclo celular y metodos de uso relacionados |
US9758790B2 (en) | 2004-12-08 | 2017-09-12 | Ceres, Inc. | Modulating the level of components within plants |
US8088975B2 (en) * | 2006-10-27 | 2012-01-03 | Ceres, Inc. | Phenylpropanoid related regulatory protein-regulatory region associations |
EP2478760A1 (en) * | 2005-05-10 | 2012-07-25 | Monsanto Technology LLC | Genes and uses for plant improvement |
US8362322B2 (en) | 2006-10-27 | 2013-01-29 | Ceres, Inc. | Modulating lignin in plants |
EP2129783A1 (en) * | 2007-03-23 | 2009-12-09 | BASF Plant Science GmbH | Transgenic plant with increased stress tolerance and yield |
WO2008120659A1 (ja) * | 2007-03-30 | 2008-10-09 | Oji Paper Co., Ltd. | 遺伝子発現情報を用いた植物の形質を判定もしくは予測する方法 |
NZ601188A (en) | 2007-12-28 | 2014-03-28 | Swetree Technologies Ab | Woody plants having improved growth characteristics and method for making the same using transcription factors |
CN102888409B (zh) * | 2012-07-31 | 2015-04-22 | 普罗米绿色能源(深圳)有限公司 | 桉树pgef10基因及其植物表达载体、宿主细胞和应用 |
CN102888410B (zh) * | 2012-07-31 | 2015-04-22 | 普罗米绿色能源(深圳)有限公司 | 桉树pgef17基因及其植物表达载体、宿主细胞和应用 |
CN102888411B (zh) * | 2012-07-31 | 2014-07-02 | 普罗米绿色能源(深圳)有限公司 | 桉树pgef13基因及其植物表达载体、宿主细胞和应用 |
BR112018006573A2 (pt) * | 2015-10-02 | 2018-10-09 | método para a produção de plantas haploides e subsequentes haploides duplicadas | |
CN110317817B (zh) * | 2019-07-16 | 2021-03-19 | 北京林业大学 | Ylb9基因序列、应用及调控植物木质素合成的方法 |
CN110885813B (zh) * | 2019-12-17 | 2021-07-27 | 中国农业大学 | 水稻组蛋白去乙酰化酶基因hda710在延迟叶片衰老中的应用 |
CN112048010B (zh) * | 2020-08-20 | 2022-06-17 | 华南农业大学 | 水稻rip2蛋白在调控植物叶夹角中的应用 |
CN112458189A (zh) * | 2020-10-24 | 2021-03-09 | 宁波国际旅行卫生保健中心(宁波海关口岸门诊部) | 一种用于单增李斯特菌荧光raa检测的引物和探针序列及其应用 |
CN113881699B (zh) * | 2021-11-05 | 2024-01-09 | 河南大学 | Mac3a和mac3b在植物器官大小调控中的应用 |
CN116218899B (zh) * | 2023-02-14 | 2024-05-31 | 中国科学院遗传与发育生物学研究所 | 水稻特异调控粒宽基因slg2及其应用 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0154204B1 (en) | 1984-03-06 | 1994-01-12 | Mgi Pharma, Inc. | Herbicide resistance in plants |
GB8626879D0 (en) | 1986-11-11 | 1986-12-10 | Ici Plc | Dna |
ES2325393T3 (es) | 1993-10-28 | 2009-09-03 | Houston Advanced Research Center | Aparato poroso de flujo a traves microfabricado para la deteccion diferenciada de reacciones de union. |
US5547861A (en) | 1994-04-18 | 1996-08-20 | Becton, Dickinson And Company | Detection of nucleic acid amplification |
US6252139B1 (en) * | 1996-07-18 | 2001-06-26 | The Salk Institute For Biological Studies | Method of increasing growth and yield in plants |
WO1999032660A1 (en) | 1997-12-19 | 1999-07-01 | Affymetrix | Exploiting genomics in the search for new drugs |
CA2266295A1 (en) | 1999-02-26 | 2000-09-19 | Heberle-Bors, Erwin | Method of modifying plant metabolism and development |
EP1163341A2 (en) | 1999-03-19 | 2001-12-19 | CropDesign N.V. | Method for enhancing and/or improving plant growth and/or yield or modifying plant architecture |
AU4459100A (en) * | 1999-04-22 | 2000-11-10 | Pioneer Hi-Bred International, Inc. | Cell cycle genes and methods of use |
CA2379850A1 (en) * | 1999-07-19 | 2001-01-25 | Japan Science And Technology Corporation | Environmental stress tolerant gene |
AU2001249622B2 (en) | 2000-03-30 | 2007-06-07 | Massachusetts Institute Of Technology | RNA sequence-specific mediators of RNA interference |
US6525319B2 (en) | 2000-12-15 | 2003-02-25 | Midwest Research Institute | Use of a region of the visible and near infrared spectrum to predict mechanical properties of wet wood and standing trees |
US6606568B2 (en) | 2000-06-28 | 2003-08-12 | Midwest Research Institute | Method for predicting dry mechanical properties from wet wood and standing trees |
CA2705037A1 (en) | 2000-10-10 | 2002-04-18 | Arborgen, Llc | Method for pine cell tissue culture on support membrane |
AR047574A1 (es) | 2003-12-30 | 2006-01-25 | Arborgen Llc 2 Genesis Res 1 | Genes del ciclo celular y metodos de uso relacionados |
-
2004
- 2004-12-29 AR ARP040104950A patent/AR047574A1/es unknown
- 2004-12-30 NZ NZ548845A patent/NZ548845A/en not_active IP Right Cessation
- 2004-12-30 US US11/024,959 patent/US7598084B2/en not_active Expired - Fee Related
- 2004-12-30 AU AU2004311384A patent/AU2004311384B2/en not_active Ceased
- 2004-12-30 BR BRPI0418229-4A patent/BRPI0418229A/pt not_active Application Discontinuation
- 2004-12-30 JP JP2006547528A patent/JP2007523636A/ja active Pending
- 2004-12-30 WO PCT/US2004/043804 patent/WO2005065339A2/en active Application Filing
- 2004-12-30 CN CNA200480042006XA patent/CN1954071A/zh active Pending
- 2004-12-30 EP EP04815805A patent/EP1711592A4/en not_active Withdrawn
-
2006
- 2006-07-26 ZA ZA200606198A patent/ZA200606198B/xx unknown
-
2009
- 2009-09-09 US US12/555,853 patent/US20100122382A1/en not_active Abandoned
-
2010
- 2010-11-10 JP JP2010251599A patent/JP2011097941A/ja active Pending
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