JP2007332092A - External preparation for skin - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a preparation by which a consumer can realize the aging-preventing function. <P>SOLUTION: The external preparation for the skin is obtained by formulating an extract of Apios americana. Medikus, and exhibits the bleaching activities and antiaging activities possessed by the extract of the Apios americana. Medikus most satisfactorily. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

The present invention relates to an external preparation for skin characterized by containing an active ingredient extracted from the American legume (Apios Americana.Medikus), and more specifically, has both a whitening action and an anti-aging action. It relates to skin care preparations.

In recent years, health consciousness has been deeply rooted, and efforts have been made to maintain an internally healthy body and spirit while paying attention to eating habits. For example, active intake of health foods and functional foods has emerged. In addition, there are many variations of supplementary foods, so-called supplements are a new form of food that is completely established in general consumers.

Under these circumstances, ingredients rich in dietary fiber are ideal for health consciousness, and their application research as health foods and functional foods is thriving, but especially potatoes are very useful as food materials that can image health. It is familiar.

In the cosmetics field, attention has been paid to the functionality of yams, and their application development has been attempted.
JP-A-6-219934 Japanese Patent Laid-Open No. 10-45615

For example, Japanese Patent Application Laid-Open No. 6-219934 discloses a whitening cosmetic based on the extract of sanjak based on a melanin production inhibitory action. Japanese Patent Application Laid-Open No. 10-45615 discloses a fibroblast growth promoter which is similarly blended with an extract of a Sanyaku of Dioscoreaceae.

In addition, the yam includes steroid saponin (dioscin) and is known to have anti-inflammatory and antioxidant effects, but skin care in the field of anti-aging is not known.

In view of these circumstances, the present inventor has developed a material that has an effective function for preventing aging without damaging the anti-inflammatory action, which is an original functional feature of yam, and has filed an application earlier. Application No. 2005-31445).

Furthermore, the present inventor has promoted research on anti-aging materials, and as a result of searching for functionality, particularly in other potato species, the anti-aging effect and whitening effect are excellent in American leguminous (Apios Americana.Medikus). And the present invention has been completed.

This invention is based on such a present condition, and it aims at providing the external preparation which mix | blended the raw material which a consumer can actually realize the antiaging effect of skin as an active ingredient.

The present inventor has continually searched to solve the above-described conventional problems and the like, and as a result, has found a specific aptitude as a desired material in the leguminous genus Apios Americana. It came to be completed.

That is, the external preparation for skin of the present invention is characterized in that it contains an active ingredient extracted from the leguminous genus American potato (Apios Americana.Medikus).

ADVANTAGE OF THE INVENTION According to this invention, the formulation which maintains a normal beautiful skin state by the whitening effect | action and the antiaging effect is provided. Hereinafter, embodiments of the present invention will be described in detail.

The skin external preparation of the present invention is characterized in that it contains an active ingredient extracted from the leguminous genus Apios Americana.Medikus. American potato (Apios Americana.Medikus), also known as potato or potato, is a rhizome of vines native to North America. It is said that farmers in Tohoku region entered Japan when they imported apple seedlings about 100 years ago.

The extract of Apios Americana.Medikus used in the present invention means an extract obtained by extracting the rhizome of Apios Americana.Medikus with a hydrophilic solvent. Below, the suitable manufacturing method of the American potato (Apios Americana.Medikus) extract which is an active ingredient of this invention is described.

American rhododendron (Apios Americana.Medikus) rhizome is dried, a hydrophilic solvent is added at a ratio of 1: 1 to 1: 100, and it is extracted by immersion or stirring at room temperature and normal pressure for 1 hour to 96 hours. The extract of the present invention can be obtained through a purification step such as filtration of the extract.

The raw American potato (Apios Americana.Medikus) is usually pulverized to about 4 mm or less in advance by a pulverizer in consideration of extraction efficiency. Since the presence or absence of the drying step does not affect the strength of the action of the extract obtained, any method can be appropriately selected and employed.

In terms of extraction efficiency, it is preferable to adjust the pH to 4.0 to 10.0 during water extraction. However, in terms of stability, when extraction is performed at pH 7.0 or higher, The most preferable condition is 4.0 to 7.0 because the stability is remarkably lowered and the product value as a cosmetic raw material is lowered due to the remarkable coloring. Furthermore, in order to increase the extraction efficiency of the active ingredient, setting of conditions such as heating and heating extraction is arbitrary.

Any extraction solvent can be used as long as it is a hydrophilic solvent. However, in consideration of practicality, one or more selected from water and lower alcohols are preferable. In view of the fact that the anti-aging effect is exhibited effectively, one or more selected from water, ethanol and 1,3-butylene glycol are particularly preferred.

The extract thus obtained may be used as it is, but is usually used by blending it with a preparation. The form of the preparation can be provided for external use, but a base generally acceptable for external preparation for skin is selected and applied directly to the affected area. In this case, in addition to homogeneous preparations represented by lotions and essences, general emulsification systems such as O / W and W / O types represented by creams and emulsions, W / O / W, O / W / O-type special multi-layer emulsions, other types such as pastes, ointments and tinctures, sprays such as aerosols and sprays, patch types such as poultices and plasters, etc. The basic base is also used in combination with other components and is not particularly limited.

In these present inventions, the amount of American potato extract (Apios Americana.Medikus) extract is in the case of cosmetics such as creams, lotions, emulsions, packs, lotions, essences, sheet masks, poultices, plasters, etc. In any case of use as a dosage form, it is blended in the range of 0.001 to 20% by weight, preferably 0.1 to 10% by weight, based on the whole preparation. When the blending amount is less than 0.001% by weight, the whitening action and anti-aging action are insufficient. Even if it exceeds 20% by weight, there is no particular difference in effect from the case of less than that, and there is a problem that this is economically disadvantageous.

In the present invention, various known active ingredients that are commonly used, for example, various vitamins known as anti-aging agents, flavonoids, kojic acid known as a whitening agent, quercetin, glutathione, hydroquinone and derivatives thereof , Condensed tannins, phenolic compounds such as caffeic acid and ellagic acid, peripheral vasodilators such as vitamin E, vitamin E nicotinate, nicotinic acid, nicotinic acid amide, various vitamins such as benzyl nicotinate, Pepper tincture, anti-inflammatory agents such as adrenal cortex hormone, ε-aminocaproic acid, lysozyme chloride, glycyrrhizin, allantoin, and other animals, plants and microorganisms such as placenta extract, licorice extract, purple root extract, and lactic acid bacteria culture extract Effects of the present invention on various extracts derived from Within a range not to impair, it can be used by adding appropriate according to prevailing purpose.

Furthermore, in addition to these known active ingredients, the skin external preparation of the present invention, in addition to base ingredients such as fats and oils, known moisturizers, antiseptics, antioxidants, chelating agents, pH adjustments as necessary Various additives such as an agent, a fragrance, and a colorant can be used in combination as long as the effects of the present invention are not impaired.

EXAMPLES Next, the present invention will be described by way of examples, but these disclosures show preferred embodiments of the present invention and do not limit the present invention in any way.

<Production Example 1>
Apios (Apios Americana.Medikus) rhizomes were heated at 90 ° C. for 1 hour, then chopped and dried. 10 kg of water was added to 0.6 kg of the dried sample and immersed for 96 hours at room temperature, and this was filtered to obtain about 9 kg of extract.

<Production Example 2>
Apios (Apios Americana.Medikus) rhizomes were heated at 90 ° C. for 1 hour, then chopped and dried. 10 kg of 50% ethanol was added to 0.6 kg of the dried sample and immersed for 96 hours at room temperature, and this was filtered to obtain about 9 kg of an extract.

<Production Example 3>
Apios (Apios Americana.Medikus) rhizomes were heated at 90 ° C. for 1 hour, then chopped and dried. 10 kg of ethanol was added to 0.6 kg of the dried sample and immersed for 96 hours at room temperature, and this was filtered to obtain about 9 kg of an extract.

<Production Example 4>
Apios (Apios Americana.Medikus) rhizomes were heated at 121 ° C. for 20 minutes, then chopped and dried. 10 kg of 1,3-butylene glycol was added to 0.6 kg of the dried sample and immersed for 96 hours at room temperature, and 21 kg of water was added to the filtrate obtained by filtering this to obtain about 30 kg of extract.

<Test Example 1> Whitening action (whitening action of mouse melanoma B16 cells)
a) Test method
10 ml of Eagle's MEM medium containing 10 v / v% fetal bovine serum was placed in a culture dish, and 0.5 × 10 ⁵ B16 cells were seeded and cultured at 37 ° C. in a 5% CO ₂ gas phase. After 24 hours of culture, the sample of the present invention was added to a final concentration of 5.0 to 11.0%. Similarly, as a comparative example, kojic acid was added at 2.5 mmol / L and 3.5 mmol / L. Moreover, the sample-free addition was used as a control. The medium was changed once on the third day of culture, and after culturing for 5 days, the cells were detached from the culture dish and centrifuged (about 1000 rpm) to prepare a cell pellet. The degree of whiteness of the cell pellet was visually observed and judged according to the following criteria. Moreover, the cell number after culture | cultivation was measured and the cell viability was computed.
[Criteria for whitening degree]
-: Black (same as control)
±: Black to gray (lighter than control)
+: Gray 2+: Gray to white

b) Test results The results of the tests are shown in Table 1. As shown in Table 1, the melanin production inhibitory action was recognized by the extract of Apios (manufacture example 1 thru | or 3).

<Test Example 2> Anti-aging effect (radical scavenging effect)
a) Test method Radical scavenging action was measured using diphenylpicrylhydrazyl (DPPH), which is a stable free radical, as a model of unsaturated fatty acid radicals. 2 mL of 0.1 mol / L acetate buffer solution (pH 5.5) and 0.5 mmol / L DPPH ethanol are added to 2 mL of the sample stock solution of the present invention or a solution diluted with ethanol to a concentration of 75%, 50%, or 25%. 1 mL of the solution was added, and after standing for 30 minutes, the absorbance was measured at a wavelength of 517 nm, and the radical scavenging rate was calculated by the following formula.
Radical scavenging rate (%) =
100 × (control OD−sample OD) / control OD

b) Test results The results of the tests are shown in Table 2. As shown in Table 2, a radical scavenging action was observed in Apios extracts (Production Examples 1 to 4).

<Test Example 3> Anti-aging effect (antioxidant effect)
a) Test method 100 mmol / L linoleic acid ethanol solution 1.0 mL, 0.1 mol / L phosphate buffer solution (pH 7.0) 6.0 mL, ethanol 1.0 mL, water 1.0 mL, sample solution 1.0 mL were mixed. And used as a test solution. The test solution was incubated at 40 ° C., and after 1 day, 2 days, 3 days, and 6 days, the peroxide value was measured by the rodan iron method to evaluate the antioxidant effect. As the sample solution, the sample of the present invention was added so that the final concentration was 0.1 to 10.0%. Similarly, as a comparative example, α-tocopherol was added to 8 μmol / L. Moreover, what added water was made into control.
Rhodan iron method: To 0.1 mL of test solution, add 4.7 mL of 75% ethanol, 0.1 mL of 30% rhodanammonium, 0.1 mL of 3.5% hydrochloric acid solution of 0.02 mmol / L ferrous chloride, Later, the absorbance at 500 nm was measured and used as the peroxide value.

b) Test result As a result of the test, in the control, the peroxide value increased due to the decomposition of linoleic acid with the passage of days, but when the Apios extract (Production Examples 1 to 4) was added, the increase in the peroxide value was suppressed. It showed strong antioxidant effect.

Formulation Example 1 Lotion (wt%)
1. American potato extract (Production Example 1) 3.00
2. Allantoin 0.10
3. Ethanol 15.00
4). Citric acid 0.10
5). P-Hydroxybenzoate 0.30
6.1,3-Butylene glycol 4.00
7). Purified water

Formulation Example 2 Essence (wt%)
1. American potato extract (Production Example 2) 1.00
2. Quercetin 0.05
3. Hyaluronic acid 5.00
4.2% Aqueous Kefiran Aqueous Solution 1.50
5). Retinoic acid 0.01
6). Glycerin 2.00
7). P-Hydroxybenzoate 1.00
8.1% carboxyvinyl polymer aqueous solution 2.00
9. Edetate disodium 0.03
10. Purified water

Formulation Example 3 Cream (% by weight)
1. American potato extract (Production Example 3) 0.10
2. Arbutin 0.05
3. Polyoxyethylene cetyl ether 2.50
4). Glycerol monostearate 1.50
5). Behenyl alcohol 5.00
6). Liquid paraffin 7.00
7). Cetyl octanoate 5.00
8). Methyl polysiloxane 0.50
9. P-Hydroxybenzoate ester 0.70
10. Carboxyvinyl polymer 0.05
11. Xanthan gum 0.01
12 Edetate disodium 0.01
13. Purified water

Formulation Example 4 Cream Pack (wt%)
1. American potato extract (Production Example 2) 0.50
2. α-Glucosyl-L-ascorbic acid 3.00
3. Polyethylene glycol 1500 5.00
4). Stearic acid diethanolamide 5.00
5). Stearic acid 5.00
6). Myristic acid 5.00
7). Coconut oil 15.00
8). Natural vitamin E 0.04
9. P-Hydroxybenzoate 0.20
10. dl-Pyrrolidonecarboxylate solution 5.00
11. Vitamin A 0.01
12 Edetate disodium calcium 0.05
13. Purified water

Formulation Example 5 Emulsion (wt%)
1. American potato extract (Production Example 4) 0.50
2. Kojic acid 5.00
3. α-Glucosyl-L-ascorbic acid 2.00
4). Octyldodecanol 3.00
5). Polyoxyethylene oleyl ether (20E.O.) 1.00
6). Stearic acid 0.50
7). Shea Butter 1.00
8). Avocado oil 4.00
9. P-Hydroxybenzoate 0.20
10. Quince Seed Extract 5.00
11. Xanthan gum 0.15
12 Phytic acid 0.02
13. d-δ-tocopherol 0.01
14 Purified water

It was confirmed that all of these Formulation Examples 1 to 5 have the effect of achieving the object of the present invention.

Claims (3)

A topical skin preparation characterized by blending an active ingredient extracted from the American legume (Apios Americana.Medikus). 2. The skin external preparation according to claim 1, wherein the extraction solvent is one or more selected from water and lower alcohols. The skin external preparation according to claim 1, wherein the lower alcohol is ethanol or 1,3-butylene glycol.