JP2007254357A - Carrot-based health medicine - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a carrot-based health medicine with enhanced pharmacological effect, thus more effective for lifestyle-related diseases increasing as the aged society comes. <P>SOLUTION: The carrot-based health medicine is obtained by including at least one of Atractylodes rhizome, Bupleurum Root and Phellodendron Bark. This medicine gives markedly increased anti-inflammatory and anti-allergic effects. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a carrot main drug health medicine containing carrot as a main drug.

BACKGROUND ART A carrot main ingredient health medicine based on medicinal carrots has been conventionally known as a nourishing tonic of a crude drug main ingredient.

“Pharmaceutical Manufacturing and Sales Guidelines 2005” issued by Jiho Co., Ltd. on October 1, 2005

Due to the recent arrival of an aging society and the enhancement of eating habits, the development of products focusing on prevention is required for the prevention of lifestyle-related diseases.
Therefore, the present invention makes the action of carrot main drug health drug more effective, and lifestyle-related diseases that increase in an aging society such as prevention of diabetes, improvement of hypertension and arteriosclerosis, prevention of aging, improvement of immunity, etc. The purpose is to provide health medicines corresponding to

As a result of various studies on the combination of carrot main ingredient health medicine to achieve the above-mentioned problems, the present inventors have found that the combination of juniper, psycho, and agaric enhances the pharmacological effect of carrot main ingredient health medicine. It came to be completed.
Sandalwood is blended in gastrointestinal drugs, cold medicines, antitussives, etc. as a healthy stomach medicine. Psycho is blended in cold medicine for the purpose of antipyretic, and is often blended in traditional Chinese medicines such as saikokuedo and shosaikoto, and has liver amelioration and anti-inflammatory effects. Awaku is used for laxatives, bitter taste stomach medicines, digestives, etc., but there is no precedent for carrots.

  The present invention is a prescription in which at least one of juniper, psycho and agaric is added to the carrot main ingredient health medicine. It is not limited by other components, and can be widely used for general carrot main health medicine. The dosage form and dosage form are not limited, and can be provided in various states such as tablets, granules, pills, and liquids.

  With these pharmacological effects, the carrot main health medicine of the present invention prevents or controls diseases such as diabetes, aging, hypertension, arteriosclerosis, cold disease, inflammation, and allergies, and further immunizes with the combination of peanuts, psychos, and acorns. It becomes a useful carrot active medicine with potentiating action.

  The above object, other objects, features, and advantages of the present invention will become more apparent from the following description of the best mode for carrying out the invention.

Examples will be specifically described below, but the present invention is not limited to these examples. First, prescriptions for carrot main drug health medicines were prepared, and this prescription (prescription B: comparative example) and powdered pea roots (the roots of pokeweed or horned radish [powdered]) were prepared. , Two products of a prescription (formulation A: Example 1) to which Psycho (powdered root of Mishima Psycho) and Aubaku (powder excluding perch of yellowfin) were added were prepared, Comparison was made by pharmacological tests.
1. Formulation A (Example 1) (A mixture of carrot main ingredient health care agent, peanut powder, psycho and agate powder, and added appropriate additives)
Carrot extract powder is blended with ogi, licorice powder, cinnamon powder, goo, jiou, peony powder, shrimp powder, processed daisan, touki powder, bakuryo powder in the following blending ratio, and peanut powder powder, psycho and powdered powder. Then, after adding a suitable additive, it was compressed into a certain shape and granulated, and then a lubricant was added and compression molded. The weight of one test substance is about 63 mg.
Carrot extract powder 450mg (Drug substance equivalent amount JP Carrot powder 3,015mg)
400 mg of ogi
Daylily powder 150mg
Keihi powder 300mg
Gooh 10mg
Ziou 300mg
Peonies powder 300mg
150g of powdered end
Processing Daisan 60mg
Toki powder 300mg
Bukuryu powder 300mg
Sandalwood powder 300mg
Psycho 200mg
Oowaku powder 200mg
2. Formula B (Comparative Example) (Prescription A excluding peanut powder, psycho and powdered powder)
Carrot extract powder 450mg (Drug substance equivalent amount JP Carrot powder 3,015mg)
400 mg of ogi
Daylily powder 150mg
Keihi powder 300mg
Gooh 10mg
Ziou 300mg
Peonies powder 300mg
150g of powdered end
Processing Daisan 60mg
Toki powder 300mg
Bukuryu powder 300mg

Pharmacological action 1
We tried to evaluate the tonic effect of nourishment by the aging preventive effect. Evaluations on aging prevention were carried out by radical scavenging activity (diabetes, hypertension, cancer, aging, allergies, gastric ulcers) and advanced glycation products (AGE: involved in aging and diabetic complications) production inhibition test (in vitoro).
(A) DPPH radical scavenging activity test
A 0.1 M acetic acid-sodium acetate buffer solution (pH 5.5) 1.0 ml, an ethanol solution of 200 lM 1,1-Diphenyl-2-pycrylhydazyl (DPPH) radical 0.5 ml, and an ethanol solution 1.0 ml of each concentration of the test substance were mixed. After standing at room temperature for 30 minutes, the absorbance was measured at 517 nm, and the concentration (SC ₅₀ ) of the test substance required to eliminate DPPH radicals (final concentration 40 lM) to 50% was calculated from the change in absorbance. The test substance was dissolved in DMSO and then diluted with ethanol for use (DMSO final concentration 0.2%). Α-tocopherol and (+)-catechin were used as comparative control drugs.
(B) AGE production inhibition test
200 mg of D-glucose, bovine serum albumin (BSA, 20 mg) and 2.0 ml of a test substance 67 mM phosphate buffer (pH 7.2) were cultured at 60 ° C. for 48 hours. The amount of AGE produced is determined by adding 2.0 ml of water to 200 ll of the reaction solution and measuring the fluorescence intensity at an excitation wavelength of 370 nm and a measurement wavelength of 440 nm. The test substance was dissolved in DMSO and then diluted with a phosphate buffer (DMSO final concentration 2.5%). (+)-Catechin and aminoguanidine were used as comparative control drugs.
As a result, the 70% ethanol extract of prescription A showed a mild radical scavenging activity and a weak AGE production inhibitory activity, and thus an anti-aging effect was observed.

Pharmacological action 2
We tried to evaluate the tonic effect of nourishment by the prevention and treatment effect of diabetes. The aldose reductase inhibitory action test was performed.
Wistar male rats were homogenized in 135 mM phosphate buffer (pH 7.0) containing 10 mM 2-mercaptoethanol and centrifuged (100,000 rpm, 30 min, 4 ° C.). The supernatant was used as a crude enzyme. The crude enzyme was dissolved in a phosphate buffer (pH 7.0) and diluted to a concentration at which about 10 nmol of NADP was produced under the following reaction conditions. The reaction mixture was 180mM phosphate buffer in _{500ll (pH7.0), 100mMLi 2 SO} 4, 0.03mMNADPH, as substrate 1mMDL-glyceraldehyde, was prepared containing DMSO solution 25ll enzyme fraction 100ll and test samples. The reaction was initiated by the addition of 30 ° C. NADPH. After incubation for 30 minutes, 0.5 MHcl 150 ll was added to stop the reaction. After stopping the reaction, 0.5 ml of 6 mM NaOH containing 10 mM imidazole is added and heated at 60 ° C. for 20 minutes to convert NADP into a fluorescent substance and measure the fluorescence intensity at an excitation wavelength of 360 nm and a fluorescence wavelength of 460 nm. A 50% inhibitory concentration (IC ₅₀ ) is calculated from the obtained fluorescence intensity. Epalrestat was used as a comparative control drug.
As a result, the 70% ethanol extract of prescription A showed a mild aldose reductase inhibitory activity, and thus has a preventive / therapeutic effect on diabetes.

Pharmacological action 3
A norepinephrine contraction test was conducted for vasodilatory effect in order to evaluate nourishing tonic effect, blood pressure lowering action or blood circulation improving action.
The thoracic aorta of Wistar male rats (body weight 250-450 g) was removed, and a spiral strip having a width of about 2 mm and a length of about 10 mm was prepared. This was attached to a Magnus apparatus filled with 6 ml of Krebs-Henselite solution and stabilized for about 1 hour. In the presence of Nifedipine (1 lM), norepinephrine (NE: 1 lM) was added for contraction, followed by washing with nutrient solution. This operation was repeated again to confirm that the contraction was stable. Next, after pre-treatment with the test substance for 10 minutes, NE (1 lM) was added to cause contraction. The average shrinkage rate in the control group was 100, and the shrinkage inhibition rate (%) of the test substance was determined. A test substance dissolved in DMS was added (final concentration: 0.1%). Nitroprusside sodium was used as a comparative control substance.
As a result, it was shown that the 70% ethanol extract of prescription A slightly suppressed NE contraction, so that a blood pressure lowering action or a blood circulation improving action was observed.

Pharmacological test 4
We tried to evaluate the tonic effect of nourishment by anti-inflammatory and anti-allergic effects. PCA reaction inhibitory effect was examined using prescription A and prescription B carrot main drug health drugs using mice.
Anti-DNP IgE (20 lg / ml PBS) diluted in ddy male mice (25-30 g body weight) was injected intradermally into both ears 10 ll at a time, and 47 hours later, the test drug (suspended in gum arabic powder) was given orally (However, they were fasted for 16 to 20 hours before administration of the test drug). One hour later, 2% Evans-blue in which DNP-BSA was dissolved as an antigen (1 mg / ml) was intravenously administered at a dose of 0.25 ml / animal. After 30 minutes, the animals were euthanized, the auricles were cut out and dissolved with 1 M KOH solution. To this, 4.5 ml of acetone-0.2M-phosphoric acid mixture (13: 5 v / v) was added and mixed. After centrifugation, the absorbance of the supernatant was measured (absorbance wavelength: 620 nm). As a comparative control drug, tranilast or ketotifen fumarate was used.

  In Formula A, administration of 0.7 g / kg and 1.4 g / kg significantly suppressed the PCA reaction using the mouse pinna. Moreover, in the prescription B, the tendency for the suppression rate to weaken was seen. It has been clarified that peanuts, psychos and acorns contribute significantly to anti-inflammatory and anti-allergic effects.

  As described above, the combination of peanut, psycho, and apricot has a function of enhancing various pharmacological effects of the carrot main drug health drug, and at least one of them may be added as follows.

1. About the composition of peanuts The amount of peanuts is 2g (extract is 5g) as a gastrointestinal standard II column I, healthy stomach, and 200mg or more as an adjuvant. (1g-0.2g)
The maximum daily amount is 2g (extract is 5g) in the standard IV column for cold medicine, and 200mg or more as an active medicine. (1g-0.2g)
In the standard II column of the laxatives, the types of the active ingredient and the daily dosage are 1 g to 0.1 g.
From the above results, the blending amount of sandalwood powder as an adjuvant is in the range of 100 to 1000 mg, 0.22 to 2.2 parts by weight with respect to carrot extract powder, or 0.03 to 0.003 with respect to carrot powder. It is suitable to mix 33 parts by weight.

2. About Psycho Compounding Psycho compounding amount as a supplement ranges from 100 to 1000 mg, 0.22 to 2.2 parts by weight for carrot extract powder, or 0.03 to 0.33 weight for carrot powder. It is suitable to mix part.

3. About the formulation of Aoba powder The maximum daily dose is 3 g (extract 3 g) as a column II item I healthy stomach medicine in the gastrointestinal drug standard, and 300 mg or more as an active medicine, but in the case of this application prescription, Keihi・ There is no lower limit for blending ginger, carrots, sandals, etc. (0-1.5g)
It is 1.5g-0.15g as a kind and a compounding quantity of the adjective ingredient in the standard II column of a laxative.
From the above results, the blending amount of Aoba powder as an adjuvant is in the range of 100 to 1500 mg, 0.22 to 3.3 parts by weight with respect to carrot extract powder, or 0.03 to 0.3 with respect to carrot powder. It is suitable to mix 5 parts by weight.

  This invention is a prescription which mix | blends a peanut, a psycho, and a buckwheat in a carrot main ingredient health medicine. It is not restricted by other components (herbal medicines) and can be widely used for general carrot active medicines. The raw material may be any of herbal medicines, herbal powders, fluid extracts, tinctures and the like, and can be administered as oral preparations such as tablets, pills, granules, capsules and liquids.

Claims (5)

  A carrot main ingredient health medicine characterized by blending at least one of peanuts, psychos, and agate.   The carrot main ingredient health medicine of Claim 1 which mix | blended the sandalwood powder 0.22-2.2 weight part with respect to the carrot extract powder, or 0.03-0.33 weight part with respect to the carrot powder.   The carrot main ingredient health of Claim 1 or Claim 2 which mix | blended Psycho with 0.22-2.2 weight part with respect to the carrot extract powder, or 0.03-0.33 weight part with respect to the carrot powder. medicine.   4. The powdered buckwheat powder according to claim 1, wherein 0.22 to 3.3 parts by weight of carrot extract powder or 0.03 to 0.5 parts by weight of carrot powder is blended. Carrot main health medicine. Mixing peanut powder, 0.22-2.2 parts by weight with respect to carrot extract powder, or 0.03-0.33 parts by weight with respect to carrot powder,
Psycho is blended in an amount of 0.22 to 2.2 parts by weight with respect to carrot extract powder, or 0.03 to 0.33 parts by weight with respect to carrot powder.
The carrot main ingredient health medicine of Claim 1 which mix | blended 0.22-3.3 weight part with respect to the carrot extract powder, or 0.03-0.5 weight part with respect to the carrot powder.