JP2007131562A - Taste-improving agent for edible portion of plant - Google Patents

Taste-improving agent for edible portion of plant Download PDF

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JP2007131562A
JP2007131562A JP2005325035A JP2005325035A JP2007131562A JP 2007131562 A JP2007131562 A JP 2007131562A JP 2005325035 A JP2005325035 A JP 2005325035A JP 2005325035 A JP2005325035 A JP 2005325035A JP 2007131562 A JP2007131562 A JP 2007131562A
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yeast cell
cell wall
plant
taste
agent
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Takanori Kitagawa
隆徳 北川
Kenji Shirai
建史 白井
Tomoko Tsuchiya
智子 土屋
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Asahi Breweries Ltd
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Asahi Breweries Ltd
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a taste-improving agent for tasting components, enhancing sugar content, improving balance between sweetness and acidity and improving Umami components of such as amino acids and the like in edible portions of agricultural products. <P>SOLUTION: The taste of edible portion of a plant is improved by giving a yeast cell wall-decomposition product prepared by decomposing yeast cell walls with an enzyme or the like to nursery seedlings and/or seedlings after fix planting to the field. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

本発明は、植物の種子・根・茎・葉面もしくは果実に、溶液状態、もしくは固体状態で葉面散布、土壌散布、土壌潅水、土壌潅注等の方法で、又は水耕栽培等の培養液に添加する方法で投与する植物可食部の食味改良剤に関する。   The present invention relates to plant seeds, roots, stems, foliage or fruits, in a solution state or in a solid state, foliar spraying, soil spraying, soil irrigation, soil irrigation, etc., or a culture solution such as hydroponics It is related with the taste improving agent of the edible part of a plant administered by the method of adding to.

野菜や工芸作物、果樹などの農作物を含む植物は、最終的に農作物として一般消費者の飲食に供されるため、農業生産の現場においてはその食味(香り、糖度、酸味、色調、硬度など)の向上が求められている。   Plants including crops such as vegetables, craft crops, fruit trees, etc. are finally used for food and drink by general consumers as crops, so the taste (aroma, sugar content, sourness, color, hardness, etc.) in the field of agricultural production Improvement is demanded.

農業生産において、農作物の健全育成を図ることは重要課題であり、農作物を含む植物の品質を向上させるために、植物生長促進剤などが用いられている。しかし、化学合成によって製造された植物生長促進剤等の薬剤は、植物の生長に著しい効果がある反面、環境に対する負荷等の影響が出てしまう可能性がある。特に、無機化合物などを用いる場合、人体への影響や植物自体に与える影響、或いは果実に対する影響等も考慮して、その使用量などを厳密に制限することが必要であった。特に、近年では食材として用いられる野菜や果実、工芸作物などの安全性が問われており、化学物質を利用しない植物の栽培方法(有機農法)等が着目されている。
特に、禾穀類、いも類、野菜、根菜、葉菜、果菜、果樹等の農作物(あるいは工芸作物)は食味の向上が求められている。食味については甘味(糖度)や酸味またはうま味成分であるアミノ酸などの成分が可食部の成分として多く含まれるか、あるいはバランスよく調整されることにより、食材のおいしさを一般消費者に提供できるものである。
In agricultural production, it is an important issue to promote healthy growth of crops, and plant growth promoters and the like are used to improve the quality of plants including crops. However, chemicals such as plant growth promoters produced by chemical synthesis have a significant effect on plant growth, but may have an impact on the environment. In particular, when an inorganic compound or the like is used, it is necessary to strictly limit the amount of use in consideration of the effect on the human body, the effect on the plant itself, the effect on the fruit, and the like. In particular, in recent years, safety of vegetables, fruits and craft crops used as foods has been questioned, and attention has been focused on plant cultivation methods (organic farming methods) that do not use chemical substances.
In particular, crops (or craft crops) such as cereals, potatoes, vegetables, root vegetables, leaf vegetables, fruit vegetables, fruit trees, etc. are required to have improved taste. With regard to the taste, it is possible to provide general consumers with the deliciousness of the ingredients by containing many components such as amino acids, which are sweetness (sugar content), sourness or umami as an edible component, or adjusted in a balanced manner. Is.

これまで、植物の生長促進剤としては、リボ核酸もしくはリボ核酸分解物を10〜40%を含有する酵母エキスにプロリン又はウラシルをそれぞれ0.5〜20%添加したものを禾穀類の幼穂形成の前後より出穂までの期間に、あるいは果菜、根菜、花卉、果樹などへの肥料として施用することを特徴とする禾穀類、果菜、根菜、花卉、果樹等の増収方法が開示されている(特許文献1)。   Until now, as plant growth promoters, yeast extracts containing 10-40% ribonucleic acid or ribonucleic acid degradation products, with 0.5-20% proline or uracil added, are used for the formation of young ears of cereals. A method for increasing the yield of cereals, fruit vegetables, root vegetables, flower buds, fruit trees, etc., characterized by being applied as a fertilizer to fruit vegetables, root vegetables, flower buds, fruit trees, etc. before and after heading is disclosed (patent document) 1).

特開昭63-45211号公報JP 63-45211

本発明においては、農作物の可食部の糖度の上昇、甘味と酸味のバランス向上、アミノ酸などのうま味成分の向上など、呈味性成分の向上を含む食味改良剤が求められている。   In the present invention, there is a need for a taste improver that includes an improvement in taste components such as an increase in sugar content in the edible part of agricultural products, an improvement in the balance between sweetness and sourness, and an improvement in umami components such as amino acids.

本発明者等は、上記課題を解決するために鋭意検討した結果、酵母細胞壁を酵素等で分解して得られた酵母細胞壁分解物を幼苗、及び/又は畑へ定植後の苗に与えることにより、植物可食部の食味を改良できることを見出し、本発明を完成させるに至った。
すなわち、本発明は、酵母細胞壁分解物を含む植物可食部の食味改良剤を提供する。
また、本発明は、植物可食部の食味を改良するための酵母細胞壁分解物の使用を提供する。
さらに、本発明は、植物活性剤に酵母細胞壁分解物を添加することを特徴とする、該植物活性剤の植物可食部の食味改良効果を向上させる方法を提供する。
また、本発明は、前記植物可食部の食味改良剤を幼苗及び/又は定植後の苗に与えることを含む栽培方法を提供する。
なお、本明細書において、「植物」は、植物の語自体から認識され得るもの、例えば穀物、種子、球根、草花、野菜、根菜、葉菜、果菜、いも類、果実、果樹、香草(ハーブ)、光合成能を有する単細胞生物、分類学上の植物等を意味するものとする。
また、「可食部」とは、一般の食品流通過程において食用として用いられる部位のことを指し、主に果樹・果菜の場合は果実部、根菜の場合は根部、葉菜の場合は葉部、穀物の場合はイネ、ムギなどの種子部分、いも類の場合は塊茎、塊根部である。
As a result of intensive studies to solve the above-mentioned problems, the present inventors have provided a yeast cell wall degradation product obtained by degrading the yeast cell wall with an enzyme or the like to young seedlings and / or seedlings after planting in a field. The present inventors have found that the taste of the plant edible portion can be improved and have completed the present invention.
That is, this invention provides the taste improving agent of the plant edible part containing a yeast cell wall degradation product.
Moreover, this invention provides use of the yeast cell wall degradation product for improving the taste of a plant edible part.
Furthermore, this invention provides the method of improving the taste improvement effect of the plant edible part of this plant active agent characterized by adding a yeast cell wall degradation product to a plant active agent.
Moreover, this invention provides the cultivation method including giving the taste improving agent of the said plant edible part to the seedling and / or the seedling after planting.
In the present specification, the “plant” can be recognized from the word of the plant itself, for example, cereal, seed, bulb, flower, vegetable, root vegetable, leaf vegetable, fruit vegetable, potato, fruit, fruit tree, herb (herb) ), Unicellular organisms with photosynthetic ability, taxonomic plants and the like.
In addition, “edible part” refers to a part that is used for food in the general food distribution process, mainly fruit part for fruit trees and fruit vegetables, root part for root vegetables, leaf part for leaf vegetables. In the case of cereals, seed parts such as rice and wheat, and in the case of potatoes, they are tubers and tuberous roots.

本発明により、植物品質を改良し、特に植物可食部の食味の改善を可能とすることが期待される、植物可食部の食味改良剤を提供することができる。   ADVANTAGE OF THE INVENTION By this invention, the plant quality can be improved and the taste improving agent of a plant edible part expected to enable improvement of the taste of a plant edible part can be provided.

本発明の植物可食部の食味改良剤は酵母細胞壁分解物を含む。
酵母細胞壁分解物は、例えば酵母細胞壁を、酵素等で処理することによって得ることができる。酵母細胞壁として、酵母そのものを用いてもよく、又は自己消化法(酵母菌体内に本来あるタンパク質分解酵素等を利用して菌体を可溶化する方法)、酵素分解法(微生物や植物由来の酵素製剤を添加して可溶化する方法)、熱水抽出法(熱水中に一定時間浸漬して可溶化する方法)、酸あるいはアルカリ分解法(種々の酸あるいはアルカリを添加して可溶化する方法)、物理的破砕法(超音波処理や、高圧ホモジェナイズ法、グラスビーズ等の固形物と混合して混合・磨砕することにより破砕する方法)、凍結融解法(凍結・融解を1回以上行うことにより破砕する方法)等により得られた細胞壁、あるいは酵母から酵母エキスを抽出した後の残渣を用いてもよい。
本発明で使用する酵母としては、分類学上あるいは工業利用上酵母と称されるものであれば特に制限はなく、ビール酵母、パン酵母、清酒酵母、ウイスキー酵母、焼酎酵母、その他アルコール発酵用酵母等が挙げられる。
酵母細胞壁を分解する酵素としては、グルカナーゼ、α−アミラーゼ、β−アミラーゼ、グルコアミラーゼ、プルラナーゼ、トランスグルコシダーゼ、デキストラナーゼ、グルコースイソメラーゼ、セルラーゼ、ナリンギナーゼ、ヘスペリジナーゼ、キシラナーゼ、ヘミセルラーゼ、マンナナーゼ、ペクチナーゼ、インベルターゼ、ラクターゼ、キチナーゼ、リゾチーム、イヌリナーゼ、キトサナーゼ、α-ガラクトシダーゼ、プロテアーゼ、パパイン、ペプチダーゼ、アミノペプチダーゼ、リパーゼ、ホスホリパーゼ、フィターゼ、酸性フォスファターゼ、ホスホジエステラーゼ、カタラーゼ、グルコースオキシダーゼ、ペルオキシダーゼ、タンナーゼ、ポリフェノールオキシダーゼ、デアミナーゼ、ヌクレアーゼなどの工業的に利用できる酵素を用いることができる。例えば、グルカナーゼを含む任意の酵素を用いることができ、市販されているツニカーゼ(大和化成(株)製)、YL-NL及びYL-15(いずれも天野エンザイム(株)製)等を用いることができる。酵母細胞壁を分解する酵素の添加量は、酵母細胞壁乾物重量に対し、一般に0.00001〜10000重量%、好ましくは0.01〜10重量%、より好ましくは0.1〜2重量%である。前記酵素により酵母細胞壁を分解する際の条件は、使用する酵素の種類、酵素の添加量等に応じて、当業者によって適宜決定すればよい。
一方、酵母細胞壁の分解には、酵素分解法以外に、50MPaの高圧ホモジナイザーでの分解や、熱水抽出、酵母細胞壁分解菌(例えばPseudomonas paucimobilis、Arthrobacter luteusなど)を接種し酵母細胞壁分解物を得ることができる。
The edible part taste improver of the present invention contains a yeast cell wall degradation product.
The yeast cell wall degradation product can be obtained, for example, by treating the yeast cell wall with an enzyme or the like. As the yeast cell wall, yeast itself may be used, or a self-digestion method (a method of solubilizing cells using a proteolytic enzyme or the like inherent in yeast cells), an enzyme decomposition method (an enzyme derived from a microorganism or a plant) (Methods of solubilization by adding preparations), hot water extraction method (methods of solubilization by soaking in hot water for a certain period of time), acid or alkali decomposition methods (methods of solubilization by adding various acids or alkalis) ), Physical crushing method (sonication, high-pressure homogenization method, crushing method by mixing with solids such as glass beads, mixing and grinding), freeze-thawing method (freezing and thawing at least once) A cell wall obtained by a method such as crushing) or a residue after extracting a yeast extract from yeast may be used.
The yeast used in the present invention is not particularly limited as long as it is referred to as a yeast for taxonomic or industrial use. Beer yeast, baker's yeast, sake yeast, whiskey yeast, shochu yeast, other yeast for alcohol fermentation Etc.
Enzymes that break down the yeast cell wall include glucanase, α-amylase, β-amylase, glucoamylase, pullulanase, transglucosidase, dextranase, glucose isomerase, cellulase, naringinase, hesperidinase, xylanase, hemicellulase, mannanase, pectinase, invertase , Lactase, chitinase, lysozyme, inulinase, chitosanase, α-galactosidase, protease, papain, peptidase, aminopeptidase, lipase, phospholipase, phytase, acid phosphatase, phosphodiesterase, catalase, glucose oxidase, peroxidase, tannase, polyphenol oxidase, deaminase, nuclease Industrially available fermentation such as Elementary can be used. For example, any enzyme including glucanase can be used, and commercially available tunicase (manufactured by Daiwa Kasei Co., Ltd.), YL-NL and YL-15 (both manufactured by Amano Enzyme Co., Ltd.), etc. can be used. it can. The amount of the enzyme that degrades the yeast cell wall is generally 0.00001 to 10,000% by weight, preferably 0.01 to 10% by weight, more preferably 0.1 to 2% by weight, based on the dry weight of the yeast cell wall. Conditions for degrading the yeast cell wall with the enzyme may be appropriately determined by those skilled in the art according to the type of enzyme used, the amount of enzyme added, and the like.
On the other hand, in order to decompose yeast cell walls, in addition to enzymatic digestion, digestion with 50MPa high-pressure homogenizer, hot water extraction, yeast cell wall degrading bacteria (eg Pseudomonas paucimobilis, Arthrobacter luteus, etc.) is obtained to obtain yeast cell wall degradation products be able to.

本発明の植物可食部の食味改良剤は、単独で用いてもよく、また農薬、肥料、園芸用培養土等と組み合わせて用いてもよい。また、特定防除資材に添加して用いてもよい。近年では天然由来から得られた病害虫・雑草防除作用又は植物生理機能を増進する成分について特定防除資材として認可する政策もとられている。この特定防除資材とは特定農薬とも呼ばれ、農薬取締法に基づき指定された農業用資材である。特定防除資材(特定農薬)は原材料に照らし農作物等、人畜及び水産動植物に害を及ぼすおそれがないことが明らかであると確認された農薬でなければならず、(1)病害虫や雑草に対する防除効果又は農作物等の生理機能の増進若しくは抑制の効果が確認されること、(2)農作物等、人畜及び水産動植物への安全性が確認されることが満たされていなければならない。
また、本発明の植物可食部の食味改良剤の形態は、液状、粉状、顆粒状等のいずれの形態で製品化してもよい。また、散布に関しては、上記製品を直接散布しても、あるいは水等で適当な濃度になるように希釈して散布してもよい。さらに、散布方法も特に限定されず、例えば、植物の種子、葉、茎等に直接散布する方法、培養苗や順化苗の葉茎に直接散布(潅水、潅注)する方法、土壌中に散布する方法等のいずれであってもよい。なお、肥料中に配合する場合、肥料としては、窒素、燐酸、カリウムを含有する化学肥料、油カス、魚カス、骨粉、海藻粉末、アミノ酸、糖類、ビタミン類などの有機質肥料等、その種類は限定されない。
The taste improver of the plant edible portion of the present invention may be used alone or in combination with agricultural chemicals, fertilizers, cultivated soil for gardening, and the like. Moreover, you may add and use for a specific control material. In recent years, a policy has been adopted in which a pest / weed control action or a component that enhances plant physiology obtained from a natural source is approved as a specific control material. This specific control material is also called a specific pesticide, and is an agricultural material designated under the Agricultural Chemicals Control Law. Specified control materials (special pesticides) must be pesticides that have been confirmed to have no risk of harm to human livestock and aquatic animals and plants, such as crops, in the light of raw materials. (1) Control effects against pests and weeds Or it must be satisfied that the effects of promoting or suppressing physiological functions of crops, etc. are confirmed, and (2) the safety of crops, etc. for human livestock and aquatic animals and plants is confirmed.
Moreover, you may commercialize the form of the taste improving agent of the plant edible part of this invention in any forms, such as liquid form, powder form, and granular form. As for spraying, the product may be sprayed directly, or may be sprayed after diluting to an appropriate concentration with water or the like. Furthermore, the spraying method is not particularly limited, for example, a method of spraying directly on the seeds, leaves, stems, etc. of plants, a method of spraying directly on the stems of cultured seedlings and acclimated seedlings (irrigation, irrigation), spraying in soil Any method may be used. In addition, when blended in fertilizers, fertilizers include chemical fertilizers containing nitrogen, phosphoric acid, potassium, oil residues, fish residues, bone meal, seaweed powder, organic fertilizers such as amino acids, sugars, vitamins, etc. It is not limited.

本発明の植物可食部の食味改良剤には、酵母細胞壁分解物の植物可食部の食味向上に対する効果を妨げない範囲で、水溶性溶剤、界面活性剤等の成分を配合することができる。
水溶性溶剤としては、エチレングリコール、ジエチレングリコール、ポリエチレングリコール、プロピレングリコールなどの2価アルコールや、グリセリンのような3価アルコール等が挙げられる。
The taste improver of the plant edible part of the present invention can be blended with components such as a water-soluble solvent and a surfactant as long as the effect of improving the taste of the plant edible part of the yeast cell wall degradation product is not hindered. .
Examples of the water-soluble solvent include dihydric alcohols such as ethylene glycol, diethylene glycol, polyethylene glycol, and propylene glycol, and trihydric alcohols such as glycerin.

界面活性剤としては、非イオン界面活性剤、陽イオン界面活性剤、両性界面活性剤及び陰イオン界面活性剤等水に溶解するものが使用できる。
非イオン界面活性剤としては、ソルビタン脂肪酸エステル、ポリオキシアルキレンソルビタン脂肪酸エステル、ポリオキシアルキレン脂肪酸エステル、グリセリン脂肪酸エステル、ポリオキシアルキレングリセリン脂肪酸エステル、ポリグリセリン脂肪酸エステル、ポリオキシアルキレンポリグリセリン脂肪酸エステル、ショ糖脂肪酸エステル、樹脂酸エステル、ポリオキシアルキレン樹脂酸エステル、ポリオキシアルキレンアルキルエーテル、ポリオキシアルキレンアルキルフェニルエーテル、アルキル(ポリ)グリコシド、ポリオキシアルキレンアルキル(ポリ)グリコシド等が挙げられる。好ましくは、窒素原子を含まないエーテル基含有非イオン界面活性剤及びエステル基含有非イオン界面活性剤が挙げられる。特に好ましくは、ポリオキシアルキレンソルビタン脂肪酸エステル、ポリオキシアルキレン脂肪酸エステル、ポリオキシアルキレングリセリン脂肪酸エステル、ポリオキシアルキレンポリグリセリン脂肪酸エステル等のオキシアルキレン基を含むエステル基含有非イオン界面活性剤や、アルキル(ポリ)グリコシド等の糖骨格を有する窒素原子を含まないエーテル基含有非イオン界面活性剤が挙げられる。
As the surfactant, nonionic surfactants, cationic surfactants, amphoteric surfactants, anionic surfactants and the like that can be dissolved in water can be used.
Nonionic surfactants include sorbitan fatty acid esters, polyoxyalkylene sorbitan fatty acid esters, polyoxyalkylene fatty acid esters, glycerin fatty acid esters, polyoxyalkylene glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyalkylene polyglycerin fatty acid esters, Examples thereof include sugar fatty acid esters, resin acid esters, polyoxyalkylene resin acid esters, polyoxyalkylene alkyl ethers, polyoxyalkylene alkyl phenyl ethers, alkyl (poly) glycosides, polyoxyalkylene alkyl (poly) glycosides, and the like. Preferably, an ether group-containing nonionic surfactant and an ester group-containing nonionic surfactant containing no nitrogen atom are used. Particularly preferably, an ester group-containing nonionic surfactant containing an oxyalkylene group such as polyoxyalkylene sorbitan fatty acid ester, polyoxyalkylene fatty acid ester, polyoxyalkylene glycerin fatty acid ester, polyoxyalkylene polyglycerin fatty acid ester, alkyl ( An ether group-containing nonionic surfactant that does not contain a nitrogen atom and has a sugar skeleton such as poly) glycoside.

陰イオン界面活性剤としては、カルボン酸系、スルホン酸系、硫酸エステル系及びリン酸エステル系界面活性剤が挙げられる。好ましくは、カルボン酸系及びリン酸エステル系界面活性剤である。カルボン酸系界面活性剤としては、例えば炭素数6〜30の脂肪酸又はその塩、多価カルボン酸塩、ポリオキシアルキレンアルキルエーテルカルボン酸塩、ポリオキシアルキレンアルキルアミドエーテルカルボン酸塩、ロジン酸塩、ダイマー酸塩、ポリマー酸塩、トール油脂肪酸塩等が挙げられる。スルホン酸系界面活性剤としては、例えばアルキルベンゼンスルホン酸塩、アルキルスルホン酸塩、アルキルナフタレンスルホン酸塩、ナフタレンスルホン酸塩、ジフェニルエーテルスルホン酸塩、アルキルナフタレンスルホン酸の縮合物塩、ナフタレンスルホン酸の縮合物塩等が挙げられる。硫酸エステル系界面活性剤としては、例えばアルキル硫酸エステル塩、ポリオキシアルキレンアルキル硫酸エステル塩、ポリオキシアルキレンアルキルフェニルエーテル硫酸エステル塩、トリスチレン化フェノール硫酸エステル塩、ポリオキシアルキレンジスチレン化フェノール硫酸エステル塩、アルキルポリグリコシド硫酸塩等が挙げられる。リン酸エステル系界面活性剤として、例えばアルキルリン酸エステル塩、アルキルフェニルリン酸エステル塩、ポリオキシアルキレンアルキルリン酸エステル塩、ポリオキシアルキレンアルキルフェニルリン酸エステル塩等が挙げられる。前記塩としては、例えば金属塩(Na、K、Ca、Mg、Zn等)、アンモニウム塩、アルカノールアミン塩、脂肪族アミン塩等が挙げられる。   Examples of the anionic surfactant include carboxylic acid-based surfactants, sulfonic acid-based surfactants, sulfate ester-based surfactants, and phosphate ester-based surfactants. Preferred are carboxylic acid and phosphate ester surfactants. Examples of the carboxylic acid-based surfactant include fatty acids having 6 to 30 carbon atoms or salts thereof, polyvalent carboxylates, polyoxyalkylene alkyl ether carboxylates, polyoxyalkylene alkylamide ether carboxylates, rosinates, Dimer acid salt, polymer acid salt, tall oil fatty acid salt and the like can be mentioned. Examples of sulfonic acid surfactants include alkylbenzene sulfonates, alkyl sulfonates, alkyl naphthalene sulfonates, naphthalene sulfonates, diphenyl ether sulfonates, alkyl naphthalene sulfonic acid condensates, and naphthalene sulfonic acid condensations. Examples include physical salts. Examples of sulfate surfactants include alkyl sulfate esters, polyoxyalkylene alkyl sulfate salts, polyoxyalkylene alkyl phenyl ether sulfate salts, tristyrenated phenol sulfate salts, polyoxyalkylene distyrenated phenol sulfate esters. Examples thereof include salts and alkyl polyglycoside sulfates. Examples of the phosphate ester surfactant include alkyl phosphate ester salts, alkylphenyl phosphate ester salts, polyoxyalkylene alkyl phosphate ester salts, and polyoxyalkylene alkylphenyl phosphate ester salts. Examples of the salt include metal salts (Na, K, Ca, Mg, Zn, etc.), ammonium salts, alkanolamine salts, aliphatic amine salts, and the like.

両性界面活性剤としては、アミノ酸系、ベタイン系、イミダゾリン系、アミンオキサイド系が挙げられる。アミノ酸系としては、例えばアシルアミノ酸塩、アシルサルコシン酸塩、アシロイルメチルアミノプロピオン酸塩、アルキルアミノプロピオン酸塩、アシルアミドエチルヒドロキシエチルメチルカルボン酸塩等が挙げられる。ベタイン系としては、アルキルジメチルベタイン、アルキルヒドロキシエチルベタイン、アシルアミドプロピルヒドロキシプロピルアンモニアスルホベタイン、アシルアミドプロピルヒドロキシプロピルアンモニアスルホベタイン、リシノレイン酸アミドプロピルジメチルカルボキシメチルアンモニアベタイン等が挙げられる。イミダゾリン系としては、アルキルカルボキシメチルヒドロキシエチルイミダゾリニウムベタイン、アルキルエトキシカルボキシメチルイミダゾリウムベタイン等が挙げられる。アミンオキサイド系としては、アルキルジメチルアミンオキサイド、アルキルジエタノールアミンオキサイド、アルキルアミドプロピルアミンオキサイド等が挙げられる。
上記界面活性剤は、単独で、又は二種以上混合して使用してもよい。
Examples of amphoteric surfactants include amino acids, betaines, imidazolines, and amine oxides. Examples of the amino acid system include acyl amino acid salts, acyl sarcosine salts, acyloylmethylaminopropionates, alkylaminopropionates, acylamidoethylhydroxyethylmethylcarboxylates, and the like. Examples of the betaine type include alkyl dimethyl betaine, alkyl hydroxyethyl betaine, acylamidopropyl hydroxypropyl ammonia sulfobetaine, acylamidopropyl hydroxypropyl ammonia sulfobetaine, ricinoleic acid amidopropyl dimethylcarboxymethyl ammonia betaine, and the like. Examples of the imidazoline series include alkyl carboxymethyl hydroxyethyl imidazolinium betaine, alkyl ethoxy carboxymethyl imidazolium betaine, and the like. Examples of amine oxides include alkyldimethylamine oxide, alkyldiethanolamine oxide, alkylamidopropylamine oxide, and the like.
You may use the said surfactant individually or in mixture of 2 or more types.

本発明の植物可食部の食味改良剤は、更に、ペプチド、多糖類、糖タンパク質及び脂質から選ばれるエリシター活性を有する物質の一種以上を含有するものを添加することもできる。エリシター活性とは、植物体内におけるファイトアレキシン等の抗菌性物質の合成を誘発する作用である。
エリシター活性を有する物質は、植物に固有の物質が種々知られており、対象とする植物に応じて適宜選定すればよいが、グルカンオリゴ糖、キチンオリゴ糖、キトサンオリゴ糖、ヘプタ−β−グルコシド、システミン、カゼインタンパクのキモトリプシン分解物などの外因性エリシター、オリゴガラクチュロン酸、ヘキソース、ウロン酸、ペントース、デオキシヘキソースなどの内因性エリシター、その他に、ショ糖エステル、カルボキシメチルセルロース(CMC)、カラギーナン、真菌類の菌糸分解物、海藻抽出物などが挙げられ、水溶性で安定供給可能なものが好ましい。
The plant edible part taste improver of the present invention may further contain a substance containing one or more substances having elicitor activity selected from peptides, polysaccharides, glycoproteins and lipids. The elicitor activity is an action that induces synthesis of an antibacterial substance such as phytoalexin in the plant body.
Various substances unique to plants are known as substances having elicitor activity, and may be appropriately selected according to the target plant. However, glucan oligosaccharide, chitin oligosaccharide, chitosan oligosaccharide, hepta-β-glucoside , Exogenous elicitors such as cystemin and chymotrypsin degradation products of casein protein, endogenous elicitors such as oligogalacturonic acid, hexose, uronic acid, pentose, deoxyhexose, sucrose ester, carboxymethylcellulose (CMC), carrageenan And mycelial decomposition products of fungi, seaweed extracts and the like, and those which are water-soluble and can be stably supplied are preferred.

本発明の植物可食部の食味改良剤は、更に、植物生長調節剤を添加することもできる。植物生長調節剤としては、オーキシン拮抗剤としては、マレイン酸ヒドラジド剤、ウニコナゾール剤等、オーキシン剤としては、インドール酪酸剤、1-ナフチルアセトアミド剤、4-CPA剤等、サイトカイニン剤としては、ホルクロルフェニュロン剤等、ジベレリン剤としてはジベレリン剤等、その他のわい化剤としては、ダミノジット剤等、蒸散抑制剤としては、パラフィン剤等、その他の植物生長調整剤としては、コリン剤等、生物由来の植物生長調整剤としては、クロレラ抽出物剤等、エチレン剤としては、エテホン剤等が挙げられる。   The plant edible part taste improver of the present invention may further contain a plant growth regulator. As plant growth regulators, auxin antagonists include maleic acid hydrazide, uniconazole, etc., auxins include indole butyric acid, 1-naphthylacetamide, 4-CPA, etc., and cytokinin as forchlor. Phenuron, etc., gibberellin as gibberellin, etc., other dwarfing agents, such as daminogit, transpiration as paraffin, etc. as other plant growth regulators, choline, etc. Examples of the plant growth regulator derived from the chlorella extract agent and the like, and the ethylene agent include an ethephon agent and the like.

本発明の植物可食部の食味改良剤は、酵母細胞壁分解物を乾物として0.00001〜30質量%、特に0.001〜0.1質量%含有することが好ましい。また、本発明の植物可食部の食味改良剤は、好ましくは酵母細胞壁分解物を乾物として10アール当たり10〜800gで与えられ、より好ましくは10アール当たり50〜250gで与えられる。上記範囲内で与えることで、より有効な植物可食部の食味改良効果を得ることができる。   The taste improver of the plant edible part of the present invention preferably contains 0.00001 to 30% by mass, particularly 0.001 to 0.1% by mass of the yeast cell wall degradation product as a dry matter. Moreover, the taste improving agent of the plant edible part of the present invention is preferably given at 10 to 800 g per 10 ares, more preferably 50 to 250 g per 10 ares, with the yeast cell wall degradation product as dry matter. By giving within the said range, the more effective taste improvement effect of a plant edible part can be acquired.

また、植物活性剤に、酵母細胞壁分解物を添加することで、植物活性剤の植物可食部の食味改良効果を向上させることができる。用いる植物活性剤は、公知の植物活性剤であればいずれであってもよい。具体的には、エチレン剤、オーキシン剤(インドール酪酸、エチクロゼート剤、クロキシホナック剤、ジクロルプロップ剤、1−ナフチルアセトアミド剤、4−CPA剤など)、オーキシン拮抗剤(マレイン酸ヒドラジド剤など)、サイトカイニン剤(ベンジルアミノプリン剤、ホルクロルフェニュロン剤など)、ジベレリン剤、ジベレリン生合成阻害剤(イナベンフィド剤、ウニコナゾールP剤、クロルメコート剤、パクロブトラゾール剤、フルルプリミドール剤、メピコートクロリド剤、プロヘキサジオンカツシウム塩剤、トリネキサパックエチル剤など)、矮化剤(ダミノジッド剤、イマザピル剤など)、イソプロチオラン剤、オキシン硫酸塩剤、過酸化カルシウム剤、シアナミド剤、塩化カルシウム・硫酸カルシウム剤、コリン剤、デシルアルコール剤、ピペロニルブトキシド剤(ピペルニブトキサイド剤)ペンディメタリン剤、MCPA剤、MCPB剤、NAC剤(カルバリル剤)、キノキサリン系・DEP剤、ピラフルフェンチル剤、プロヒドロジャスモン剤、アブシジン酸剤、クロレラ抽出物、シイタケ菌糸体抽出物などが挙げられる。植物活性剤への酵母細胞壁分解物の添加量は、酵母細胞壁分解物を乾物として0.00001〜30質量%の範囲が好ましく、特に0.001〜0.1質量%の範囲が好ましい。   Moreover, the taste improvement effect of the plant edible part of a plant active agent can be improved by adding a yeast cell wall degradation product to a plant active agent. The plant activator used may be any known plant activator. Specifically, ethylene agent, auxin agent (indole butyric acid, ethiclozate agent, cloxyphonac agent, dichloroprop agent, 1-naphthylacetamide agent, 4-CPA agent, etc.), auxin antagonist (maleic hydrazide agent, etc.) , Cytokinins (benzylaminopurine, forchlorphenuron, etc.), gibberellins, gibberellins biosynthesis inhibitors (inabenfide, uniconazole P, chlormecote, paclobutrazole, flurprimidol, mepi Coat chloride agent, prohexadione calcium salt agent, trinexapac ethyl agent, etc.), dwarfing agent (daminozide agent, imazapyr agent, etc.), isoprothiolane agent, oxine sulfate agent, calcium peroxide agent, cyanamide agent, calcium chloride・ Calcium sulfate, choline, Sil alcohol, piperonyl butoxide (pipernibutoxide) pendimethalin, MCPA, MCPB, NAC (carbaryl), quinoxaline / DEP, pyraflufentil, prohydrojasmon, absidine An acid agent, a chlorella extract, a shiitake mycelium extract, etc. are mentioned. The addition amount of the yeast cell wall degradation product to the plant active agent is preferably in the range of 0.00001 to 30% by mass, particularly preferably in the range of 0.001 to 0.1% by mass with the yeast cell wall degradation product as a dry matter.

本発明の植物可食部の食味改良剤を幼苗、及び/又は畑へ定植後の苗に与えることにより、植物品質の改良を行い、特に植物可食部の呈味性を向上させることができる。   By giving the plant edible part taste improver of the present invention to young seedlings and / or seedlings after planting in the field, plant quality can be improved, and in particular, the taste of the plant edible part can be improved. .

(酵母細胞壁液1の調製)
ビール醸造後の酵母を原料とし、自己消化法により得られた、乾物濃度15質量%の酵母液1.5Lから遠心分離により上清を除去し、酵母細胞壁スラリー1000gを得た。水500gを加え、pHを5.5に調整後、乾物質量に対し0.7%のYL-15(天野エンザイム)を添加し、55℃で18時間反応させ、80℃で10分間処理した後に1500gの酵母細胞壁液1を得た。
(Preparation of yeast cell wall fluid 1)
Using the yeast after brewing as a raw material, the supernatant was removed by centrifugation from 1.5 L of a yeast solution having a dry matter concentration of 15% by mass obtained by the self-digestion method to obtain 1000 g of yeast cell wall slurry. After adding 500 g of water and adjusting the pH to 5.5, add 0.7% of YL-15 (Amano Enzyme) to the dry matter, react at 55 ° C for 18 hours, treat at 80 ° C for 10 minutes, and then 1500 g of yeast cell wall Liquid 1 was obtained.

(実施例1)
イチゴ(品種:ペチカ)苗を定植し、EC0.6に調整した養液土耕1号(大塚化学)を施用し、1週間後に、養液土耕1号(大塚化学)に乾物濃度10ppmとなるように酵母細胞壁液1を混入した液肥を施肥した。定植後、70日目に果実の収穫を行い、果実の糖度、酸度、糖酸比の測定を行った。
Example 1
Strawberry (variety: Petica) seedlings were planted, and nourishment soil culture No. 1 (Otsuka Chemical) adjusted to EC 0.6 was applied, and one week later, nourishment soil culture No. 1 (Otsuka Chemical) had a dry matter concentration of 10 ppm. The liquid fertilizer mixed with the yeast cell wall fluid 1 was applied. Fruits were harvested on the 70th day after planting, and the sugar content, acidity, and sugar acid ratio of the fruits were measured.

(実施例2)
イチゴ(品種:ペチカ)苗を定植し、EC0.6に調整した養液土耕1号(大塚化学)を施用し、1週間後に、養液土耕1号(大塚化学)に乾物濃度100ppmとなるように酵母細胞壁液1を混入した液肥を施肥した。定植後、70日目に果実の収穫を行い、果実の糖度、酸度、糖酸比の測定を行った。
(Example 2)
Strawberry (variety: Petica) seedlings were planted, and nourishment soil culture No. 1 (Otsuka Chemical) adjusted to EC 0.6 was applied. One week later, nourishment soil culture No. 1 (Otsuka Chemical) had a dry matter concentration of 100 ppm. The liquid fertilizer mixed with the yeast cell wall fluid 1 was applied. Fruits were harvested on the 70th day after planting, and the sugar content, acidity, and sugar acid ratio of the fruits were measured.

(比較例1)
イチゴ(品種:ペチカ)苗を定植し、EC0.6に調整した養液土耕1号(大塚化学)を施用し、1週間後に、養液土耕1号(大塚化学)を施肥した。定植後、70日目に果実の収穫を行い、果実の糖度、酸度、糖酸比の測定を行った。実施例1、2及び比較例1の測定結果を表1に示す。
(Comparative Example 1)
Strawberry (variety: Petica) seedlings were planted, and nourishment soil culture No. 1 (Otsuka Chemical) adjusted to EC 0.6 was applied, and one week later, nourishment soil culture No. 1 (Otsuka Chemical) was fertilized. Fruits were harvested on the 70th day after planting, and the sugar content, acidity, and sugar acid ratio of the fruits were measured. Table 1 shows the measurement results of Examples 1 and 2 and Comparative Example 1.

表1 糖度・酸度・糖酸比(70日目)

Figure 2007131562
Table 1 Sugar content, acidity, sugar acid ratio (70th day)
Figure 2007131562

(結果)
酵母細胞壁液を施用しなかった比較例1と比較し、酵母細胞壁液を施用した実施例1〜2では糖酸比が上昇することが明らかとなった。このことから、酵母細胞壁液を施用することにより、糖酸比が上昇し、食味を向上させることが明らかとなった。
(result)
In comparison with Comparative Example 1 in which the yeast cell wall fluid was not applied, in Examples 1 and 2 in which the yeast cell wall fluid was applied, it was revealed that the sugar acid ratio was increased. From this, it was clarified that by applying the yeast cell wall fluid, the sugar acid ratio was increased and the taste was improved.

(実施例3)
イチゴ(品種:きみのひとみ)苗を定植し、定植直後に、EC0.6に調整した養液土耕1号(大塚化学)に乾物濃度250ppmとなるように酵母細胞壁液1を混入した液肥を施肥した。その後、30日毎にEC0.6に調整した養液土耕1号(大塚化学)に酵母細胞壁固形分を250ppm混入した液肥を施肥した。定植後100日目に果実の収穫を行い、熟練したパネル20名が食し、好きな方を選択する2点比較法にて行った。
(Example 3)
Strawberry (variety: Kimi no Hitomi) seedlings are planted, and immediately after planting, liquid fertilizer mixed with yeast cell wall fluid 1 to a dry matter concentration of 250ppm in nutrient solution soil culture No. 1 (Otsuka Chemical) adjusted to EC 0.6 Fertilized. Then, liquid fertilizer mixed with 250 ppm of yeast cell wall solids was applied to hydroponic soil No. 1 (Otsuka Chemical) adjusted to EC 0.6 every 30 days. Fruits were harvested on the 100th day after planting, and 20 skilled panelists ate and performed the two-point comparison method to select the favorite one.

(比較例2)
イチゴ(品種:きみのひとみ)苗を定植し、定植直後に、EC0.6に調整した養液土耕1号(大塚化学)を施肥した。その後、30日毎にEC0.6に調整した養液土耕1号(大塚化学)を施肥した。定植後100日目に果実の収穫を行い、熟練したパネル20名が食し、好きな方を選択する2点比較法にて行った。結果を表2に示す。
(Comparative Example 2)
Strawberry (variety: Kimi no Hitomi) seedlings were planted, and immediately after planting, nutrient solution soil cultivation No. 1 (Otsuka Chemical) adjusted to EC 0.6 was fertilized. After that, fertilizer Nourishment No. 1 (Otsuka Chemical) adjusted to EC 0.6 every 30 days was fertilized. Fruits were harvested on the 100th day after planting, and 20 skilled panelists ate and performed the two-point comparison method to select the favorite one. The results are shown in Table 2.

表2 好きと答えた人数

Figure 2007131562
Table 2 Number of people who answered that they liked
Figure 2007131562

(結果)
酵母細胞壁液を施用しなかった比較例2のイチゴの方がおいしいと答えたパネルが6名であったのに対し、酵母細胞壁液を施用した実施例3のイチゴの方がおいしいと答えたパネルは14名であった。クレーマーの迅速有意差検定の結果、5%水準で有意差が認められた。このことから、酵母細胞壁液を施用することにより、食味を向上させる効果があることが明らかとなった。
(result)
The panel which answered that the strawberry of the comparative example 2 which did not apply the yeast cell wall liquid was more delicious, compared with 6 persons, whereas the panel which answered that the strawberry of the example 3 which applied the yeast cell wall liquid was delicious. There were 14 people. As a result of Kramer's rapid significance test, a significant difference was found at the 5% level. From this, it was revealed that there is an effect of improving the taste by applying the yeast cell wall fluid.

(酵母細胞壁液2の調製)
ビール醸造後の酵母を原料とし、酵素分解法により得られた、乾物濃度15質量%の酵母液1.5Lから遠心分離により上清を除去し、酵母細胞壁スラリー1000gを得た。水500gを加え、pHを5.5に調整後、乾物質量に対し0.5%のYL-15(天野エンザイム)を添加し、55℃で18時間反応させ、80℃で10分間処理した後に1500gの酵母細胞壁液2を得た。
(Preparation of yeast cell wall fluid 2)
Using yeast after brewing beer as a raw material, the supernatant was removed by centrifugation from 1.5 L of a yeast solution having a dry matter concentration of 15% by mass obtained by an enzymatic decomposition method to obtain 1000 g of yeast cell wall slurry. After adding 500 g of water and adjusting the pH to 5.5, add 0.5% of YL-15 (Amano Enzyme) to the dry matter, react at 55 ° C for 18 hours, treat at 80 ° C for 10 minutes, and then 1500 g of yeast cell wall Liquid 2 was obtained.

(実施例4:育苗期1回散布)
育苗ポットにチンゲンサイ(夏栄)種子を播種し発芽させ、播種から11日目の幼苗に乾物濃度250ppmに調整した酵母細胞壁液2に浸潤した。酵母細胞壁液浸潤1週間後に定植し、3週間チンゲンサイの苗を育苗した。定植から1ヶ月経過したチンゲンサイ苗6株を無作為に抽出し、グルコース含量を測定した。
(Example 4: one seedling spraying)
Chingensai (Natsuei) seeds were sown and germinated in a seedling pot, and seedlings on the 11th day after sowing were infiltrated into yeast cell wall fluid 2 adjusted to a dry matter concentration of 250 ppm. One week after infiltration of the yeast cell wall fluid, planting was carried out for 3 weeks. Six seedling rhinoceros seedlings one month after the planting were randomly extracted and the glucose content was measured.

(実施例5:育苗期1回散布、生育期1回散布)
育苗ポットにチンゲンサイ(夏栄)種子を播種し発芽させ、播種から11日目の幼苗に乾物濃度250ppmに調整した酵母細胞壁液2に浸潤した。酵母細胞壁液浸潤から1週間後に定植し、定植後2週間目に酵母細胞壁液2の葉面散布を行い、その後2週間チンゲンサイ苗を生育させた。定植から1ヶ月経過したチンゲンサイ苗6株を無作為に抽出し、グルコース含量を測定した。
(Example 5: spraying once in the seedling season, spraying once in the growing season)
Chingensai (Natsuei) seeds were sown and germinated in a seedling pot, and seedlings on the 11th day after sowing were infiltrated into yeast cell wall fluid 2 adjusted to a dry matter concentration of 250 ppm. One week after the infiltration of the yeast cell wall fluid, the plants were planted one week later, and the leaves of the yeast cell wall fluid 2 were sprayed on the second week after the planting. Six seedling rhinoceros seedlings one month after the planting were randomly extracted and the glucose content was measured.

(実施例6:育苗期1回散布、生育期2回散布)
育苗ポットにチンゲンサイ(夏栄)種子を播種し発芽させ、播種から11日目の幼苗に乾物濃度250ppmに調整した酵母細胞壁液2に浸潤した。酵母細胞壁液浸潤から1週間後に定植した。定植後2週間目に酵母細胞壁液2の葉面散布を行い、その1週間後に2回目の酵母細胞壁液2の葉面散布を行った。定植から1ヶ月経過したチンゲンサイ苗6株を無作為に抽出し、グルコース含量を測定した。
(Example 6: spraying once in the seedling season, spraying twice in the growing season)
Chingensai (Natsuei) seeds were sown and germinated in a seedling pot, and seedlings on the 11th day after sowing were infiltrated into yeast cell wall fluid 2 adjusted to a dry matter concentration of 250 ppm. One week after infiltration of the yeast cell wall fluid, the plants were planted. Two weeks after the planting, foliar spraying of the yeast cell wall fluid 2 was performed, and one week after that, the second foliar spraying of the yeast cell wall fluid 2 was performed. Six seedling rhinoceros seedlings one month after the planting were randomly extracted and the glucose content was measured.

(比較例3)
育苗ポットにチンゲンサイ(夏栄)種子を播種し発芽させ、播種から18日目の幼苗を定植した。定植から1ヶ月経過したチンゲンサイ苗6株を無作為に抽出し、グルコース含量を測定した(表3)。
(Comparative Example 3)
Chingensai (Natsuei) seeds were sown and germinated in a seedling pot, and seedlings were planted on the 18th day after sowing. Six seedling rhinoceros seedlings 1 month after the planting were randomly extracted and the glucose content was measured (Table 3).

表3

Figure 2007131562
Table 3
Figure 2007131562

(結果)
酵母細胞壁液を施用しなかった比較例3と比較し、酵母細胞壁液を施用した実施例3〜5ではグルコース含量が増加することが明らかとなった。このことから、酵母細胞壁液を施用することにより、グルコース含量が増加し、食味を向上させることが明らかとなった。
(result)
As compared with Comparative Example 3 in which the yeast cell wall fluid was not applied, it was revealed that the glucose content increased in Examples 3 to 5 in which the yeast cell wall fluid was applied. From this, it became clear that applying the yeast cell wall fluid increases the glucose content and improves the taste.

(実施例7)
育苗用ポッドにエダマメ種子(品種:白鳥)を播種し、播種後10日目に乾物濃度250ppmに調整した酵母細胞壁液2を10分間、根に浸漬させた。播種後21日目に畑へ定植し、定植後5日目に乾物濃度250ppmに調整した酵母細胞壁液2を1株あたり10mL、根に潅注させた。定植後から2ヵ月後、無作為に選抜した5株について、糖度(Brix%)と食味を調査した。
(Example 7)
Green seeds (variety: swan) were sown in a seedling pod, and the yeast cell wall fluid 2 adjusted to a dry matter concentration of 250 ppm was immersed in the root for 10 minutes on the 10th day after sowing. On the 21st day after sowing, the plants were planted in the field, and on the 5th day after sowing, the yeast cell wall fluid 2 adjusted to a dry matter concentration of 250 ppm was irrigated to the roots at 10 mL per strain. Two months after planting, the sugar content (Brix%) and taste were investigated for 5 randomly selected strains.

(比較例4)
育苗用ポッドにエダマメ種子(品種:白鳥)を播種し、播種後10日目に水道水を10分間、根に浸漬させた。播種後21日目に畑へ定植し、定植後5日目に水道水を1株あたり10mL、を根に潅注させた。定植後から2ヵ月後、無作為に選抜した5株について、糖度(Brix%)と食味を調査した。
(Comparative Example 4)
Green soybean seeds (variety: swan) were sown in the seedling pod, and tap water was immersed in the root for 10 minutes on the 10th day after sowing. On the 21st day after sowing, the plants were planted in the field, and on the 5th day after planting, 10 mL of tap water per strain was irrigated to the roots. Two months after planting, the sugar content (Brix%) and taste were investigated for 5 randomly selected strains.

(食味評価方法)
実施例7あるいは比較例4で収穫したエダマメの食味を比較評価した。
評価は、湿重量60gのエダマメを、40gの食塩を加えた沸騰水1Lで5分間ゆでたものを熟練したパネル30名が食し、好きな方を選択する2点比較法にて行った。結果を表4及び5に示す。
(Taste evaluation method)
The taste of the green beans harvested in Example 7 or Comparative Example 4 was comparatively evaluated.
Evaluation was carried out by a two-point comparison method in which 30 skilled panel members ate boiled soybeans with a wet weight of 60 g in 1 L of boiling water added with 40 g of salt for 5 minutes, and selected the favorite one. The results are shown in Tables 4 and 5.

表4 糖度の結果

Figure 2007131562




Table 4 Sugar content results
Figure 2007131562




表5 食味評価の結果

Figure 2007131562
Table 5 Results of taste evaluation
Figure 2007131562

(結果)
正規分布表(両側確立)により検定した結果、有意(危険率5%)に実施例7で栽培し収穫したエダマメを好んでいた。
(result)
As a result of the test using the normal distribution table (both sides established), the soybeans cultivated and harvested in Example 7 were significantly (risk rate 5%).

(実施例8)
育苗用ポッドにトマト種子(品種:ルネッサンス)を播種し、乾物濃度100ppmに調整した酵母細胞壁液1を1株あたり4mL潅注した。播種から1ヵ月後、養液栽培へ定植し、EC4から6で栽培し、週に1回、乾物濃度250ppmに調整した酵母細胞壁液1を、1株あたり約1Lずつ葉面散布した。播種から3ヶ月後及び4ヶ月後に収穫した全果実について、非破壊糖度計により糖度を計測した。
(Example 8)
A seedling pod was seeded with tomato seeds (variety: Renaissance), and 4 mL of yeast cell wall fluid 1 adjusted to a dry matter concentration of 100 ppm was irrigated. One month after sowing, the plants were planted in hydroponics, cultivated at EC4 to 6, and the yeast cell wall fluid 1 adjusted to a dry matter concentration of 250 ppm was sprayed on the surface of the leaves once a week. The sugar content was measured with a non-destructive sugar content meter for all fruits harvested 3 and 4 months after sowing.

(実施例9)
育苗用ポッドにトマト種子(品種:ルネッサンス)を播種した。播種から1ヶ月後に、養液栽培へ定植し、EC4から6で栽培した。定植後、週に1回、乾物濃度250ppmに調整した酵母細胞壁液1を、1株あたり約1Lずつ葉面散布した。播種から3ヶ月後及び4ヶ月後に収穫した全果実について、非破壊糖度計により糖度を計測した。
Example 9
Tomato seeds (variety: Renaissance) were sown in a seedling pod. One month after sowing, the plants were planted in hydroponics and cultivated at EC4 to 6. After planting, the yeast cell wall fluid 1 adjusted to a dry matter concentration of 250 ppm was sprayed on the foliage about 1 L per strain once a week. The sugar content was measured with a non-destructive sugar content meter for all fruits harvested 3 and 4 months after sowing.

(実施例10)
育苗用ポッドにトマト種子(品種:ルネッサンス)を播種し、乾物濃度100ppmに調整した酵母細胞壁液1を1株あたり4mL潅注した。播種から1ヵ月後、養液栽培へ定植し、EC4から6で栽培した。播種から3ヶ月後及び4ヶ月後に収穫した全果実について、非破壊糖度計により糖度を計測した。
(Example 10)
A seedling pod was seeded with tomato seeds (variety: Renaissance), and 4 mL of yeast cell wall fluid 1 adjusted to a dry matter concentration of 100 ppm was irrigated. One month after sowing, the plants were planted in hydroponics and cultivated with EC4 to 6. The sugar content was measured with a non-destructive sugar content meter for all fruits harvested 3 and 4 months after sowing.

(比較例5)
育苗用ポッドにトマト種子(品種:ルネッサンス)を播種した。播種から1ヶ月後に、養液栽培へ定植し、EC4から6で栽培した。播種から3ヶ月後及び4ヶ月後に収穫した全果実について、非破壊糖度計により糖度を計測した。
(Comparative Example 5)
Tomato seeds (variety: Renaissance) were sown in a seedling pod. One month after sowing, the plants were planted in hydroponics and cultivated at EC4 to 6. The sugar content was measured with a non-destructive sugar content meter for all fruits harvested 3 and 4 months after sowing.

(結果)
実施例8〜10及び比較例5の可食部の糖度について、播種から3ヶ月目の前半・後半、4ヶ月目の前半・後半でそれぞれ測定した結果を図1に示す。
播種から3ヶ月目前半・後半、4ヶ月目前半・後半のいずれの時期においても、糖度は比較例5<実施例10<実施例9<実施例8の順に高くなり、酵母細胞壁液の施用回数または施用量の多かった順に糖度が高くなっていた。
(result)
About the sugar content of the edible part of Examples 8-10 and the comparative example 5, the result measured in the first half and the latter half of the 3rd month from sowing, and the first half and the latter half of the 4th month is shown in FIG.
The sugar content increases in the order of Comparative Example 5 <Example 10 <Example 9 <Example 8 and the number of times of application of the yeast cell wall fluid in both the first half and the second half of the third month from the sowing. Or the sugar content became high in the order of increasing application rate.

実施例8〜10及び比較例5のトマトの糖度の測定結果である。It is a measurement result of the sugar content of the tomatoes of Examples 8 to 10 and Comparative Example 5.

Claims (6)

酵母細胞壁分解物を含む植物可食部の食味改良剤。   Taste improving agent for plant edible parts containing yeast cell wall degradation products. 酵母細胞壁分解物を乾物として0.00001〜30質量%含む請求項1記載の植物可食部の食味改良剤。   The taste improver for plant edible parts according to claim 1, comprising 0.00001 to 30% by mass of a yeast cell wall degradation product as a dry matter. 酵母細胞壁分解物を有効成分として含む請求項1記載の植物可食部の食味改良剤。   The taste improver for plant edible parts according to claim 1, comprising a yeast cell wall degradation product as an active ingredient. 植物可食部の食味を改良するための酵母細胞壁分解物の使用。   Use of yeast cell wall degradation products to improve the taste of plant edible parts. 植物活性剤に酵母細胞壁分解物を添加することを特徴とする、該植物活性剤の植物可食部の食味改良効果を向上させる方法。   A method for improving the taste improving effect of a plant edible portion of the plant active agent, comprising adding a yeast cell wall degradation product to the plant active agent. 請求項1〜3のいずれか1項記載の食味改良剤を幼苗及び/又は定植後の苗に与えることを含む栽培方法。   The cultivation method including giving the taste improving agent of any one of Claims 1-3 to a seedling and / or seedling after planting.
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