JP2007116972A - Bone fracture preventive agent, and slipped tendon preventive agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a bone fracture preventive agent and a slipped tendon preventive agent each for pets or livestock, inexpensive and highly stable in the living body. <P>SOLUTION: The bone fracture preventive agent comprises pet food or feed containing lactobion acid and/or its calcium salt or sodium salt. Specifically, the bone fracture preventive agent is obtained by admixing 0.3-3.0 mass% of the lactobion acid to commercially available feed mixture for a first stage in a broiler-chicken fatting process to obtain feed, giving poussin of zero day old the feed for 10 days so as to enhance calcium absorption of the poussin to increase the weight of its thigh bone and the amount of the calcium of the thigh bone. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a feed or pet food containing lactobionic acid and / or a salt thereof, and further to a fracture prevention agent and a leg weakness prevention agent containing lactobionic acid and / or a salt thereof as an active ingredient. .

  Due to problems such as aging of pets such as dogs and cats due to recent advances in veterinary medicine such as the vaccination of vaccines, lack of exercise, lack of sunlight irradiation, etc., bone diseases such as osteoporosis and osteomalacia have increased in pets There is a problem with pet fractures caused by it. The livestock industry is also trying to increase the growth rate of livestock by providing feed with high nutrition efficiency to livestock such as pigs and chickens for the purpose of improving productivity. The problem of weak legs, malformations, and broken bones is occurring because the growth has not caught up with gain. In particular, leg weakness in meat chickens, when severe, leads to complete paralysis of the legs or the whole body, and is a major cause of meat chicken disposal.

  These are considered to be caused by poor availability of minerals, particularly calcium, in pet food and feed.

  In general, it is essential that the mineral is absorbed in a soluble state in the intestinal tract. Casein phosphopeptide (CPP) and poly-γ-glutamic acid are known as substances that promote the intestinal absorption of minerals by solubilizing minerals, especially calcium and iron, and feeds containing these are also known to the world. (See Patent Document 1 and Patent Document 2). However, CPP has a problem in that the ability to solubilize minerals in the intestine decreases due to dephosphorylation by alkaline phosphatase present in the small intestinal mucosa in the intestine. In addition, poly-γ-glutamic acid is not currently used in feed due to problems such as price.

On the other hand, it has been reported that lactobionic acid has a mineral absorption promoting action (Patent Document 3), but administration of lactobionic acid and / or salt thereof to pets and livestock prevented these fractures and leg weakness. There is no report.
Japanese Patent Publication No. 3-71101 JP-A-9-28309 Japanese Patent No. 3501237

  An object of the present invention is to provide a preventive agent for fractures and leg weakness in pets and livestock that can be produced stably and inexpensively in vivo.

  As a result of intensive studies to solve the above problems, the present inventors have found that the general formula is lactobionic acid represented by O-β-D-galactopyranosyl- (1-4) -D-gluconic acid and The inventors have found that the salt promotes bone mineralization by promoting calcium absorption from the small intestine, and has reached the present invention. Lactobionic acid is not enzymatically decomposed in the intestine, and can be produced at low cost by fermentation or chemical synthesis using lactose as a raw material.

  That is, the gist is a feed or pet food containing lactobionic acid and / or a salt thereof. The gist of the present invention is a fracture prevention agent containing lactobionic acid and / or a salt thereof as an active ingredient. Furthermore, the gist of the present invention is a leg weakness preventing agent containing lactobionic acid and / or a salt thereof as an active ingredient.

  ADVANTAGE OF THE INVENTION According to this invention, the fracture prevention agent and leg weakness prevention agent for pets and livestock which are highly stable and cheap in the living body are provided.

  Lactobionic acid is an aldonic acid obtained by oxidizing the aldehyde group at the reducing end of lactose, and the water solubility of calcium and iron salts is known to be much higher than that of other calcium and iron salts. In the present invention, lactobionic acid is considered to promote calcium absorption in the lower intestinal tract by forming a highly water-soluble salt with calcium in pet food and feed. In addition, lactobionic acid is an indigestible oligosaccharide that is assimilated by intestinal bacteria to generate organic acids, lowering the pH of the lower intestinal tract, and forming insoluble calcium phosphate normally formed in the lower intestinal tract. Hinder. These combined actions promote calcium absorption in pet food and feed, and as a result, promote ossification and calcification of limb bones, but the details of the mechanism are unknown.

  The lactobionic acid used in the present invention may be in the form of a salt that can be added to pet food and feed such as calcium salt and sodium salt as well as free acid.

  Lactobionic acid and / or a salt thereof used in the present invention may be synthesized by oxidizing lactose with bromine or the like. Lactose or whey may contain Acinetobacter or Burkholderia (JP 2001-245657). And the like obtained by the action of microorganisms such as Gluconobacter genus. The purity of lactobionic acid and / or its salt does not necessarily have to be high, and there is no problem even if other substances such as whey protein (peptide) and saccharide are contained.

  The content of lactobionic acid and / or salt thereof in pet food or feed is about 0.01 to 3% by mass, and preferably about 0.01 to 1% by mass.

  Pet food and feed can be used for livestock such as cattle, pigs, and horses, for poultry such as chickens, turkeys, and quails, for fish such as eels, sea breams, and breams, and for pets such as dogs, cats, and tropical fish. However, the present invention can be used for any of these applications.

  The composition of other pet food and feed ingredients other than lactobionic acid and / or its salt may be the same as the composition of the normal pet food and feed, and contains protein, carbohydrate, fat, mineral, vitamin, Calcium is 0.01 to 30% by mass, preferably 0.1 to 5% by mass. In the feed, iron may be 0.001 to 1% by mass, preferably about 0.005 to 0.05% by mass.

  Examples of the feed include cereals, algae, vegetable oil meal, animal feed, other feeds, refined products, and mixtures thereof. Examples of cereals include milo, wheat, barley, oats, rye, brown rice, buckwheat, sweet potato, millet, mackerel, corn, and soybeans. Examples of the algae include rice bran, defatted rice bran, bran, powder, wheat, germ, wheat bran, corn bran, corn germ and the like. Examples of vegetable oil cakes include soybean oil cake, kinako powder, linseed oil cake, cottonseed oil cake, peanut oil cake, safflower oil cake, palm oil cake, palm oil cake, sesame oil cake, sunflower oil cake, rapeseed oil cake, Examples include Kapok oil residue and coconut oil residue. Examples of animal feed include fish meal such as North Sea meal, imported meal, whole meal, and coastal meal, fish solubil, meat meal, meat and bone meal, blood meal, decomposed hair, bone meal, processing byproducts for livestock, feather meal, salmon, defatting Examples include powdered milk, casein, and dried whey. Other feeds include plant stems and leaves such as alfalfa, hay cube, alfalfa leaf meal, false acacia powder, processed soybean products such as soy protein, corn processing industry by-products such as corn gluten meal, corn gluten feed, corn stapler, starch Fermented industrial products such as yeast, beer ground, malt root, alcohol grounds, soy sauce ground, etc., agricultural production by-products such as citrus grounds, tofu grounds, coffee grounds, cocoa grounds, cassava, broad beans, guamile, seaweed , Krill, spirulina, chlorella and minerals. Examples of purified products include proteins such as casein and albumin, amino acids, starch, cellulose, sugars such as sucrose, glucose, minerals, vitamins, and the like.

  The production method of the pet food and feed of the present invention may be the same as the normal production method, and in the mixing process, lactobionic acid and / or salt thereof may be added simultaneously with or separately from other feed components and mixed uniformly. . Specifically, feed or pet food to which lactobionic acid and / or a salt thereof is added is fluidized bed granulation, stirring granulation, extrusion granulation, rolling granulation, airflow granulation, compression molding granulation, Granulation methods such as crushing granulation, spray granulation, spray granulation, coating methods such as pan coating, fluidized bed coating, dry coating, puff drying, excess steam method, foam mat method, microwave heating method, etc. It can be produced using an extrusion method such as an extrusion granulator or an extruder.

  The feeding method of the pet food or feed of the present invention to the pet or livestock may be a conventional method of pet food or feed. Moreover, since lactobionic acid and its salt are easily soluble in water, it can also be added to drinking water.

  In the present invention, the effect of lactobionic acid and / or its salt on preventing leg weakness and fracture of domestic animals and pets can be examined by measuring femoral weight and calcium content (%) in the femur according to conventional methods. .

Example 1
Lactobionic acid (manufactured by Wako Pure Chemical Industries, Ltd.) 0.3% by mass, 1.0% by mass, or 3.0% by mass on a commercial broiler fattening mixed feed (“Blofit A”, trade name, Kyodo feed product) Were added and mixed. In addition, as a comparative example, three types of feed were prepared by mixing the same feed with the same amount of casein phosphopeptide (CPP-I, manufactured by Meiji Seika Co., Ltd.) instead of lactobionic acid.

  Seven test plots were organized with 0 day-old meat chickens (15 each) as one test plot. Each test group was ingested with any of the above 6 types of feed and additive-free feed for 10 days, and the femur weight and femoral calcium amount (%) were measured. The feeding results are shown in Table 1.

As shown in Table 1, by adding lactobionic acid to the feed, the calcium absorption of the chicken for meat is promoted, the increase in femoral weight and the ossification of the femur are promoted, and the effect is higher than that of CPP-I. It was shown to be expensive.

Example 2
0.1% by mass of calcium lactobionate (Sigma Aldrich) was added to a commercial feed for raising piglets (“Wake Piglet 79”, trade name, cooperative feed product). Two test groups were set with weaned piglets (25 days old, 5 animals) as one test group. Each test section is fed with either calcium lactobionate-containing diet or additive-free diet from weaning to 70 days of age (30 kg), and bone density of the humerus at 70, 140, and 185 days of age Was measured by DEXA (double X-ray absorption measurement method). The results are shown in Table 2.

Claims (3)

Feed or pet food containing lactobionic acid and / or a salt thereof. A fracture prevention agent containing lactobionic acid and / or a salt thereof as an active ingredient. An agent for preventing leg weakness comprising lactobionic acid and / or a salt thereof as an active ingredient.