JP2007001924A - Skin care preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an anti-aging agent, a cell activator, a collagen synthesis promoter, a hyaluronic acid synthesis promoter and a skin care preparation, each capable of preventing and ameliorating wrinkles, fleckles, somberness, skin texture vanishment, skin resilience decline, etc., associated with skin aging by activating fibroblast constituting the dermis and thereby promoting the synthesis of collagen and hyaluronic acid. <P>SOLUTION: The anti-aging agent, the cell activator, the collagen synthesis promoter, the hyaluronic acid synthesis promoter and the skin care preparation, each containing, as active ingredient, Commelinaceae communis var. hortensis extract, are provided, respectively. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to an anti-aging agent, a cell activator, a collagen synthesis promoter, a hyaluronic acid synthesis promoter, and a skin containing an extract of C. communis var. Hortensis, which is a plant of the Commelinaceae family It relates to an external preparation.

  The dermis that constitutes the skin is mainly composed of fibroblasts and matrix components. Fibroblasts produce proteins such as collagen and glycosaminoglycans such as hyaluronic acid to form connective tissue and play an important role in the skin. Therefore, when collagen, hyaluronic acid, etc. decrease, the skin ages and wrinkles, spots, dullness, disappearance of texture, loss of elasticity, etc. occur.

  Focusing on the functions of such collagen and hyaluronic acid, hyaluronic acid contained in chicken tosaka and the like has been conventionally blended in cosmetics. However, since hyaluronic acid is a polymer, there is a problem that it is difficult to absorb even if a cosmetic containing the hyaluronic acid is directly applied to the skin. Thus, an external preparation for skin that can activate synthesis of collagen and hyaluronic acid by activating fibroblasts has been sought.

  By the way, it has been known that many plant extracts have various physiological effects. For example, in the case of the long-bellied beetle, it is known that the extract has a blood glucose rise inhibitory action (see Patent Document 1). However, it has not been known that fibroblasts are activated by applying an extract of the burrow extract to the skin and the synthesis of collagen and hyaluronic acid by the cells is promoted.

JP 2002-316935 A

  The present invention activates fibroblasts constituting the dermis and promotes the synthesis of collagen and hyaluronic acid by the cells, thereby causing wrinkles, spots, dullness, disappearance of texture, loss of elasticity, etc. accompanying aging An object of the present invention is to provide an anti-aging agent, a cell activator, a collagen synthesis promoter, a hyaluronic acid synthesis promoter, and a skin external preparation that can prevent and improve the above.

  As a result of intensive studies to solve the above-mentioned problems, it is possible to activate fibroblasts by applying an extract of sorghum on the skin, and as a result, it is possible to promote the synthesis of collagen and hyaluronic acid by the cells. As a result, the present invention has been completed.

  That is, the present invention relates to an anti-aging agent, a cell activator, a collagen synthesis promoter, a hyaluronic acid synthesis promoter, and an external preparation for skin, which contain a warbler extract as an active ingredient.

  According to the present invention, by activating the fibroblasts constituting the dermis and promoting the synthesis of collagen and hyaluronic acid by the cells, wrinkles, spots, dullness, disappearance of texture, elasticity of aging Can prevent and improve the decline.

  The present invention relates to an anti-aging agent, a cell activator, a collagen synthesis promoter, a hyaluronic acid synthesis promoter, and an external preparation for skin, which contains an extract of Syringa as an active ingredient.

  The long-horned beetle (Commelinaceae communis var. Hortensis) is a highly safe camellia plant that has been used for dyeing and food. In the present invention, an extract of burdock can be obtained from the whole plant (various parts such as stems, leaves, roots, flowers, etc.) or a part thereof, or a dried product thereof.

  Although it does not specifically limit as a solvent used for extraction, For example, water; Alcohols, such as methanol, ethanol, propanol, butanol, isopropanol, isobutanol, n-hexanol, methyl amyl alcohol, 2-ethyl butanol, n-octyl alcohol Polyhydric alcohols such as glycerin, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1,3-butylene glycol, hexylene glycol Esters such as ethyl acetate, butyl acetate, methyl propionate and methyl formate; keto such as acetone and methyl ethyl ketone S; ethyl ether, ethers such as isopropyl ether, tetrahydrofuran; hexane, toluene, chloroform, hydrocarbon solvents such as methylene chloride. These can be used alone or in combination of two or more. Among these, water and alcohols are preferable from the viewpoint of safety and convenience as a skin external preparation, and water and ethanol are more preferable.

  For example, when water and ethanol are used in combination as a solvent, an extract of S. aureus can be obtained satisfactorily even if the ratio of the two is arbitrary. Especially, when used as a cell activator, the extraction solvent has an ethanol concentration. It is preferably 0 to 100% by volume, and more preferably 0 to 50% by volume. When used as a collagen synthesis promoter, the extraction solvent preferably has an ethanol concentration of 0 to 100% by volume, more preferably 0 to 50% by volume. Furthermore, when used as a hyaluronic acid synthesis promoter, the extraction solvent preferably has an ethanol concentration of 20 to 100% by volume, more preferably 50 to 100% by volume.

  Although it does not specifically limit as an extraction method, Methods, such as immersion or heating reflux, are mention | raise | lifted. The amount of the solvent is preferably 100 to 100,000 parts by weight, and more preferably 500 to 10,000 parts by weight with respect to 100 parts by weight of the pulverized warbler. If the solvent is less than 100 parts by weight, the extraction efficiency tends to deteriorate, and even if added over 100,000 parts by weight, further improvement in the extraction efficiency cannot be expected, and the concentration and purification process takes time. It tends to be inefficient. Moreover, about temperature, when making it immerse, although temperature can also be performed at room temperature, 0-95 degreeC is preferable and it is preferable to immerse for 1 to 7 days. These extraction operations can be repeated once or two to several times.

  The obtained extract may be used as it is, but may be purified by removal of impurities, deodorization, decolorization, concentration, etc. by filtration, gel filtration, centrifugation, chromatography, distillation, distribution method, etc. . Further, the solvent can be removed by a spray drying method or a freeze drying method to obtain a dry solid form, or a form solubilized in a solvent such as purified water or an emulsion form.

  The anti-aging agent, cell activator, collagen synthesis promoter, hyaluronic acid synthesis promoter, and external preparation for skin of the present invention are produced by blending the above-mentioned extract of scallop as an active ingredient. As for the extract of burdock, it itself has cell activation, collagen synthesis promotion, hyaluronic acid synthesis promotion, etc., so the extract can be used alone, but for example, fats and oils that are usually used for bases for external use Waxes, hydrocarbons, fatty acids, lower alcohols, higher alcohols, polyhydric alcohols, esters, water-soluble polymers, surfactants, moisturizers, anti-inflammatory agents, UV absorbers, preservatives, An antifungal agent, a fragrance, a pigment, an excipient, an antioxidant, a cosmetic ingredient, a cosmetic stabilizer, and the like may be contained. Further, in addition to the above-mentioned extract of the scabbard, other cell activators, anti-aging agents, collagen synthesis promoters, hyaluronic acid synthesis promoters, and the like can be used in combination.

  Examples of the external preparation for skin of the present invention include an external preparation for improving wrinkle prevention, a cosmetic for skin aging prevention, a sunscreen cosmetic, a cosmetic for skin protection, a whitening cosmetic, a foundation and other quasi drugs. can give.

  In the present invention, the blending amount of the above-mentioned extract is not particularly limited, but is preferably 0.00001 to 10% by weight, more preferably 0.001 to 1% by weight. If the blending amount is less than 0.00001% by weight, the anti-aging effect, the cell activation effect, the collagen synthesis promoting effect, and the hyaluronic acid synthesis promoting effect tend not to be sufficiently expressed, and more than 10% by weight. However, there is a tendency that an effect corresponding to that cannot be expected.

  The anti-aging agent, cell activator, collagen synthesis promoter, hyaluronic acid synthesis promoter and skin external preparation of the present invention are in various forms such as lotion, emulsion, gel, sol, cream, ointment, powder, spray and the like. It can be. In addition, when used as a skin external preparation, especially in the form of cosmetics, skin cosmetics such as lotions, emulsions, creams, cosmetics, packs, face wash, makeup base lotions, makeup base creams, foundations Makeup cosmetics such as liquid foundation and lipstick, body cosmetics such as hand cream, leg cream, body lotion, body soap and soap, and cosmetics for hair such as shampoo, rinse and hair nourishing agent.

  Next, the present invention will be described in more detail based on examples, but the present invention is not limited thereto.

Production Example 1
The dried whole plant of C. communis var. Hortensis was cut to an appropriate size. To 1 g (dry weight) of the whole cut grass, 50 ml of purified water was added as an extraction solvent, and the mixture was left to stand in a refrigerator (4 ° C.) for 5 days for extraction. The extract was concentrated to 1/10 and lyophilized. As a dry powder, 147.5 mg of Scutella napus extract was obtained.

Production Example 2
As the extraction solvent, an extract of Oboushibana was obtained in the same manner as in Production Example 1, except that 5% ethanol aqueous solution was used instead of purified water. As a dry powder, 145.5 mg of Scutella napus extract was obtained.

Production Example 3
As the extraction solvent, an extract of Oboushibana was obtained in the same manner as in Production Example 1, except that 10% ethanol aqueous solution was used instead of purified water. As a dry powder, 145.0 mg of Scutella napus extract was obtained.

Production Example 4
As the extraction solvent, an extract of Obobushi bana was obtained in the same manner as in Production Example 1 except that 20% ethanol aqueous solution was used instead of purified water. As a dry powder, 142.5 mg of Scutella napus extract was obtained.

Production Example 5
As the extraction solvent, an extract of burdock was obtained in the same manner as in Production Example 1, except that 50% ethanol aqueous solution was used instead of purified water. As a dry powder, 119.5 mg of Scutella napus extract was obtained.

Production Example 6
As the extraction solvent, an extract of Oboushibana was obtained in the same manner as in Production Example 1, except that 80% ethanol aqueous solution was used instead of purified water. As a dry powder, 65.0 mg of Scutella napus extract was obtained.

Production Example 7
As a manufacturing solvent, an extract was obtained in the same manner as in Production Example 1 except that ethanol (100%) was used instead of purified water as an extraction solvent. As a dry powder, 23.0 mg of Scutella napus extract was obtained.

Test Example 1 (Cell activation test)
Normal human fibroblasts (NHDF: Kurashiki Boseki Co., Ltd.) were pre-cultured in Dulbecco's modified Eagle medium containing 10% FBS (cell culture conditions: CO ₂ incubator 5% CO ₂ , 95% air (hereinafter, cells) All culture conditions are the same)). After culturing, the cells were collected, the number of cells was adjusted to 2.5 × 10 ⁵ cells / ml with Dulbecco's modified Eagle medium containing 1% FBS, and 2 ml each was seeded on a 35 mm dish. After 24 hours, the culture medium was replaced with Dulbecco's modified medium (2 ml) containing 1% FBS, which contained the sorghum extract obtained in Production Examples 1 to 7 having the concentrations shown in Table 1, and cultured for 48 hours. After completion of the culture, Dulbecco's modified medium (2 ml) containing 1% FBS containing 2- (4,5-dimethyl-2-thiazolyl) -3,5-diphenyltetrazolium bromide (MTT) at a concentration of 0.2 mg / ml. And cultured for 2 hours. After 2 hours, the formazan resulting from the opening of the tetrazolium ring was extracted with 2-propanol (2 ml). The absorbance (550-650 nm) of the extract was measured and used as the amount of cell activity. The value obtained by using the A. niger extract obtained in Production Examples 1 to 7 was expressed as an index using the cell activity amount of the extract when cultured in a medium not containing the A. niger extract as a reference (100). The results are shown in Table 1. From the results shown in Table 1, it was confirmed that the extract of the scabbard of Production Examples 1 to 7 has a cell activation effect.

Test Example 2 (Collagen synthesis promoting action test)
Normal human fibroblasts (NHDF: Kurashiki Boseki Co., Ltd.) were pre-cultured in Dulbecco's modified Eagle medium containing 10% FBS. After culturing, the cells were collected, the number of cells was adjusted to 2.5 × 10 ⁵ cells / ml with Dulbecco's modified Eagle medium containing 1% FBS, and 2 ml each was seeded on a 35 mm dish. After 24 hours, the culture medium was replaced with Dulbecco's modified Eagle's medium (2 ml) containing 1% FBS, which was obtained in Preparation Examples 1 to 7 having the concentrations shown in Table 2, and cultured for 72 hours. After completion of the culture, the amount of type I procollagen in the culture supernatant was measured by ELISA using Procollagen type I C-peptide EIA Kit (manufactured by Takara Bio Inc.). Since type I procollagen is a precursor of type I collagen, the amount of type I procollagen was measured and used as a biochemical indicator of the amount of collagen synthesis. Based on the amount of type I procollagen in the culture supernatant when cultivated in a medium not containing the extract of Aspergillus niger, the value obtained using the Aspergillus extract obtained in Production Examples 1 to 7 is used as an index. displayed. The results are shown in Table 2. From the results shown in Table 2, it was confirmed that the extract of the scabbard of Production Examples 1 to 7 has a collagen synthesis promoting action. In particular, it could be confirmed that the warbler extract of Production Examples 1 to 5 has a high collagen synthesis promoting action.

Test Example 3 (hyaluronic acid synthesis promoting action test)
Normal human fibroblasts (NHDF: Kurashiki Boseki Co., Ltd.) were pre-cultured in Dulbecco's modified Eagle medium containing 10% FBS. After culturing, the cells were collected, the number of cells was adjusted to 2.5 × 10 ⁵ cells / ml with Dulbecco's modified Eagle medium containing 1% FBS, and 2 ml each was seeded on a 35 mm dish. After 24 hours, the culture medium was replaced with Dulbecco's modified Eagle's medium (2 ml) containing 1% FBS, which was obtained in Production Examples 1 to 7 having the concentrations shown in Table 3, and cultured for 72 hours. After completion of the culture, the amount of hyaluronic acid in the culture supernatant was measured by ELISA using a hyaluronic acid measurement kit (Seikagaku Corporation). Based on the amount of hyaluronic acid in the culture supernatant when cultivated in a medium not containing the extract of Syringa as a standard (100), the values obtained using the Syringa extract of Production Examples 1 to 7 were displayed as indices. . The results are shown in Table 3. From the results shown in Table 3, it was confirmed that the scab extract of Production Examples 5 to 7 has a high hyaluronic acid synthesis promoting action.

Example 1 (cream)
Components (A) and (B) shown in Table 4 were dissolved by heating at 85 ° C., and components (A) and (B) were mixed and dispersed. The component (C) was added thereto, and gradually cooled to 30 ° C. with stirring to obtain a uniform cream.

Example 2 (Liquid Foundation)
Components (A) and (B) shown in Table 5 were dissolved by heating at 85 ° C., and components (A) and (B) were mixed and dispersed. The component (C) was added thereto, and the mixture was gradually cooled to 30 ° C. with stirring to obtain a uniform liquid foundation.

Comparative Example 1 (cream)
A cream was obtained in the same manner as in Example 1 except that purified water was used in place of the sorghum extract obtained in Production Example 5.

Comparative Example 2 (Liquid Foundation)
A liquid foundation was obtained in the same manner as in Example 1 except that purified water was used in place of the sorghum extract obtained in Production Example 5.

Test Example 3 (Skin Cosmetics Monitor Test)
Twenty healthy adults in their 20s, 30s and 40s (60 people in total) were randomly selected as subjects, and the skin cosmetics of Examples 1 and 2 and Comparative Examples 1 and 2 were treated as usual. The method was used for 2 months every day.

  After 2 months, the skin condition was evaluated for the improvement effect on wrinkles, and the improvement effect on tension and sagging according to the following criteria. The results are shown in Table 6.

  In this monitoring test, no subject had an abnormality on the skin. Moreover, each skin cosmetic was stable without change in the state over two months.

[Improvement effect on wrinkles]
The condition of the eyes was visually observed and evaluated based on the following evaluation criteria.
A: It was greatly improved.
B: Improved.
C: Slightly improved.
D: There was no improvement effect.
E: Deteriorated.

[Improvement effect on beam and sagging]
The state of the skin of the entire face was visually observed and evaluated based on the following evaluation criteria.
A: It was greatly improved.
B: Improved.
C: Slightly improved.
D: There was no improvement effect.
E: Deteriorated.

  From the results shown in Table 6, when the skin external preparations of Examples 1 and 2 were used, the wrinkles and swelling of the eyes and the sagging were improved as compared with the case of using the skin external preparations of Comparative Examples 1 and 2. You can see that In other words, according to the present invention, it can be seen that the topical skin preparation containing the extract of Syringa prevents and improves wrinkles, burrs and sagging, and generally exhibits an anti-aging effect.

  Hereinafter, the formulation example of the external preparation for skin of this invention is shown. The content is% by weight.

<Prescription Example 1: Cream>
Paramecium extract 0.05
Sodium hyaluronate 0.01
Glyceryl monostearate 3.0
Polyoxyethylene (20) sorbitan monostearate 3.0
Cetanol 2.0
Squalane 3.0
Glyceryl 2-ethylhexanoate 10.0
Glycerin 7.0
Ethylparaben 0.1
Purified water balance 100

<Prescription Example 2: Cream>
Oboushibana extract 0.1
Stearic acid 10.0
Cetanol 2.0
Lanolin 1.0
Isopropyl myristate 3.0
Polyethylene glycol monostearate 1.5
Triethanolamine 0.8
Sorbitol (70%) 4.0
Methylparaben 0.1
Fragrance 0.01
Purified water balance 100

<Prescription Example 3: Liquid Foundation>
Oboushibana extract 0.01
Hyaluronic acid 0.01
Glyceryl monostearate 2.0
Polyoxyethylene (4) sodium lauryl ether phosphate 0.5
Stearic acid 5.0
Behenyl alcohol 1.0
Lanolin 2.0
Squalane 5.0
Glyceryl 2-ethylhexanoate 4.0
Pigment 10.0
Propylene glycol 7.0
Triethanolamine 1.0
Ethylparaben 0.1
Purified water balance 100

<Prescription Example 4: Liquid Foundation>
Paramecium extract 0.05
Lanolin 2.0
Liquid paraffin 5.0
Stearic acid 2.0
Cetanol 1.0
Glycerin 2.0
Squalane 5.0
Glyceryl 2-ethylhexanoate 4.0
Pigment 10.0
Propylene glycol 7.0
Triethanolamine 1.0
Ethylparaben 0.1
Fragrance 0.01
Purified water balance 100

<Prescription Example 5: Latex>
Paramecium extract 0.02
Hyaluronic acid 0.01
Stearic acid 2.0
Ethanol 0.5
Liquid paraffin 10.0
Lanolin Fatty Acid Isopropyl 3.0
Lanolin 4.0
Squalane 5.0
Sorbitan sesquiisostearate 1.0
Propylene glycol 5.0
Triethanolamine 0.6
Ethylparaben 0.1
Fragrance 0.01
Purified water balance 100

<Prescription Example 6: Latex>
Oboushibana extract 0.1
Stearic acid 3.5
Ethanol 0.5
Liquid paraffin 3.0
Lanolin 0.5
Squalane 2.0
Propylene glycol 3.0
Triethanolamine 0.8
Ethylparaben 0.1
Carboxyvinyl polymer 1% liquid (alkali neutralization) 8.0
Fragrance 0.01
Purified water balance 100

<Formulation example 7: lotion>
Paramecium extract 0.02
Sodium hyaluronate 0.01
Polyoxyethylene (20) sorbitan monolaurate 1.0
1,3-butylene glycol 3.0
Sorbitol (70%) 2.0
Sodium pyrrolidonecarboxylate solution 3.0
Ethanol 15.0
Ascorbic acid 0.1
Methylparaben 0.1
Fragrance 0.01
Purified water balance 100

<Prescription Example 8: Lotion>
Paramecium extract 0.05
Polyoxyethylene (20) sorbitan monolaurate 1.0
1,3-butylene glycol 5.0
Sorbitol (70%) 2.0
Sodium pyrrolidonecarboxylate solution 3.0
Ethanol 15.0
Ascorbic acid 0.1
Methylparaben 0.1
Dye 0.01
Fragrance 0.01
Purified water balance 100

Claims (5)

An anti-aging agent containing an extract of Aspergillus oryzae as an active ingredient. A cell activator comprising an extract of Aspergillus oryzae as an active ingredient. Collagen synthesis promoter containing an Oboushibana extract as an active ingredient. A hyaluronic acid synthesis promoter containing an extract of Aspergillus oryzae as an active ingredient. An external preparation for skin containing an extract of sorghum as an active ingredient.