JP2006520598A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2006520598A5 JP2006520598A5 JP2006507065A JP2006507065A JP2006520598A5 JP 2006520598 A5 JP2006520598 A5 JP 2006520598A5 JP 2006507065 A JP2006507065 A JP 2006507065A JP 2006507065 A JP2006507065 A JP 2006507065A JP 2006520598 A5 JP2006520598 A5 JP 2006520598A5
- Authority
- JP
- Japan
- Prior art keywords
- collagen
- biocoat
- caco
- culture
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000008186 Collagen Human genes 0.000 description 5
- 108010035532 Collagen Proteins 0.000 description 5
- 229920001436 collagen Polymers 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 102100033350 ATP-dependent translocase ABCB1 Human genes 0.000 description 2
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 102000013373 fibrillar collagen Human genes 0.000 description 2
- 108060002894 fibrillar collagen Proteins 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 238000013537 high throughput screening Methods 0.000 description 2
- 108010047230 Member 1 Subfamily B ATP Binding Cassette Transporter Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 210000001842 enterocyte Anatomy 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000004017 serum-free culture medium Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US45523603P | 2003-03-17 | 2003-03-17 | |
| PCT/US2004/007390 WO2004083384A2 (en) | 2003-03-17 | 2004-03-11 | Accelerated culture system for intestinal epithelial cell monolayers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006520598A JP2006520598A (ja) | 2006-09-14 |
| JP2006520598A5 true JP2006520598A5 (enExample) | 2007-01-25 |
Family
ID=33029974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006507065A Pending JP2006520598A (ja) | 2003-03-17 | 2004-03-11 | 腸上皮細胞単層のための促進された培養システム |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7300794B2 (enExample) |
| EP (1) | EP1606395A4 (enExample) |
| JP (1) | JP2006520598A (enExample) |
| CA (1) | CA2518496A1 (enExample) |
| WO (1) | WO2004083384A2 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100047907A1 (en) * | 2008-08-19 | 2010-02-25 | Absorption System Group LLC | Methods of transporting epithelial cell monolayers |
| JP5180978B2 (ja) * | 2010-02-01 | 2013-04-10 | 花王株式会社 | 抗肥満物質のスクリーニング方法 |
| CN102676369B (zh) * | 2011-12-19 | 2013-12-25 | 河南科技大学 | 一种膳食锌生物有效性的评价模型及其建立方法 |
| CN109971699A (zh) * | 2017-12-28 | 2019-07-05 | 首都医科大学附属北京天坛医院 | 一种原代小鼠小肠上皮细胞培养液及其制备方法和应用 |
| CN111500543B (zh) * | 2020-04-16 | 2023-05-30 | 武汉普诺赛生命科技有限公司 | 一种Caco-2细胞单层膜形成培养方法 |
| CN112111445A (zh) * | 2020-09-16 | 2020-12-22 | 浙江工商大学 | 一种利用Transwell构建小鼠肠道上皮体外单层培养及表征方法 |
| WO2025178087A1 (ja) * | 2024-02-21 | 2025-08-28 | 公立大学法人名古屋市立大学 | 腸管がん由来細胞から、小腸様の高い腸管上皮細胞様機能と形態を有する細胞を作製する方法、及びその利用 |
| CN118064371A (zh) * | 2024-04-19 | 2024-05-24 | 深圳奥礼生物科技有限公司 | 一种快速制备单层caco-2细胞渗透模型的方法 |
| CN118853540B (zh) * | 2024-09-27 | 2024-12-10 | 济南磐升生物技术有限公司 | 一种人阴道上皮模型的体外构建方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL94611A (en) * | 1989-06-05 | 1994-12-29 | Organogenesis Inc | Medium for cell cultures containing insulin or growth factor similar to insulin, transferrin or iron ion, triiodothyronine or thyroxine and method of use |
-
2004
- 2004-03-11 CA CA002518496A patent/CA2518496A1/en not_active Abandoned
- 2004-03-11 WO PCT/US2004/007390 patent/WO2004083384A2/en not_active Ceased
- 2004-03-11 US US10/797,952 patent/US7300794B2/en not_active Expired - Fee Related
- 2004-03-11 JP JP2006507065A patent/JP2006520598A/ja active Pending
- 2004-03-11 EP EP04719713A patent/EP1606395A4/en not_active Withdrawn
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Koh et al. | Molding of hydrogel microstructures to create multiphenotype cell microarrays | |
| Kim et al. | Dynamic display of biomolecular patterns through an elastic creasing instability of stimuli-responsive hydrogels | |
| Cruz et al. | Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease | |
| Li et al. | Culture of primary rat hepatocytes within porous chitosan scaffolds | |
| Kreutzer et al. | Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes | |
| Kang et al. | Liver sinusoid on a chip: Long‐term layered co‐culture of primary rat hepatocytes and endothelial cells in microfluidic platforms | |
| Lu et al. | Morphological and functional characterization and assessment of iPSC-derived hepatocytes for in vitro toxicity testing | |
| JP2006520598A5 (enExample) | ||
| CN102197129A (zh) | 细胞培养试剂盒、筛选方法和细胞培养试剂盒的制造方法 | |
| Sunami et al. | Effect of honeycomb film on protein adsorption, cell adhesion and proliferation | |
| Selden et al. | Three‐dimensional in vitro cell culture leads to a marked upregulation of cell function in human hepatocyte cell lines‐an important tool for the development of a bioartificial liver machine | |
| LaPointe et al. | Nanoscale topography and chemistry affect embryonic stem cell self‐renewal and early differentiation | |
| Frampton et al. | Rapid Self‐Assembly of Macroscale Tissue Constructs at Biphasic Aqueous Interfaces | |
| JP2013510570A5 (enExample) | ||
| Zahmatkesh et al. | In vitro modeling of liver fibrosis in 3D microtissues using scalable micropatterning system | |
| Verma et al. | Formation and characterization of three dimensional human hepatocyte cell line spheroids on chitosan matrix for in vitro tissue engineering applications | |
| Sasaki et al. | Intestinal permeability of drugs in caco-2 cells cultured in microfluidic Devices | |
| Westensee et al. | Artificial cells eavesdropping on HepG2 cells | |
| Christensen et al. | Effect of oxygen tension on the amino acid utilisation of human embryonic stem cells | |
| Wang et al. | Engineering modular 3D liver culture microenvironments in vitro to parse the interplay between biophysical and biochemical microenvironment cues on hepatic phenotypes | |
| Zhang et al. | Generation of self-renewing hepatoblasts from human embryonic stem cells by chemical approaches | |
| BéruBé et al. | Filter-well technology for advanced three-dimensional cell culture: perspectives for respiratory research | |
| JP2013226112A (ja) | 肝細胞の培養方法 | |
| Shrirao et al. | Vacuum-assisted fluid flow in microchannels to pattern substrates and cells | |
| Zhong et al. | Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation |