CA2518496A1 - Accelerated culture system for intestinal epithelial cell monolayers - Google Patents
Accelerated culture system for intestinal epithelial cell monolayers Download PDFInfo
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- CA2518496A1 CA2518496A1 CA002518496A CA2518496A CA2518496A1 CA 2518496 A1 CA2518496 A1 CA 2518496A1 CA 002518496 A CA002518496 A CA 002518496A CA 2518496 A CA2518496 A CA 2518496A CA 2518496 A1 CA2518496 A1 CA 2518496A1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0679—Cells of the gastro-intestinal tract
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
- C12N2501/392—Sexual steroids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/02—Drug screening
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US45523603P | 2003-03-17 | 2003-03-17 | |
| US60/455,236 | 2003-03-17 | ||
| PCT/US2004/007390 WO2004083384A2 (en) | 2003-03-17 | 2004-03-11 | Accelerated culture system for intestinal epithelial cell monolayers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2518496A1 true CA2518496A1 (en) | 2004-09-30 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002518496A Abandoned CA2518496A1 (en) | 2003-03-17 | 2004-03-11 | Accelerated culture system for intestinal epithelial cell monolayers |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7300794B2 (enExample) |
| EP (1) | EP1606395A4 (enExample) |
| JP (1) | JP2006520598A (enExample) |
| CA (1) | CA2518496A1 (enExample) |
| WO (1) | WO2004083384A2 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010022151A1 (en) * | 2008-08-19 | 2010-02-25 | Absorption Systems Group Llc | Methods of transporting epithelial cell monolayers |
| JP5180978B2 (ja) * | 2010-02-01 | 2013-04-10 | 花王株式会社 | 抗肥満物質のスクリーニング方法 |
| CN102676369B (zh) * | 2011-12-19 | 2013-12-25 | 河南科技大学 | 一种膳食锌生物有效性的评价模型及其建立方法 |
| CN109971699A (zh) * | 2017-12-28 | 2019-07-05 | 首都医科大学附属北京天坛医院 | 一种原代小鼠小肠上皮细胞培养液及其制备方法和应用 |
| CN111500543B (zh) * | 2020-04-16 | 2023-05-30 | 武汉普诺赛生命科技有限公司 | 一种Caco-2细胞单层膜形成培养方法 |
| CN112111445A (zh) * | 2020-09-16 | 2020-12-22 | 浙江工商大学 | 一种利用Transwell构建小鼠肠道上皮体外单层培养及表征方法 |
| WO2025178087A1 (ja) * | 2024-02-21 | 2025-08-28 | 公立大学法人名古屋市立大学 | 腸管がん由来細胞から、小腸様の高い腸管上皮細胞様機能と形態を有する細胞を作製する方法、及びその利用 |
| CN118064371A (zh) * | 2024-04-19 | 2024-05-24 | 深圳奥礼生物科技有限公司 | 一种快速制备单层caco-2细胞渗透模型的方法 |
| CN118853540B (zh) * | 2024-09-27 | 2024-12-10 | 济南磐升生物技术有限公司 | 一种人阴道上皮模型的体外构建方法 |
Family Cites Families (1)
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| CA2018228C (en) * | 1989-06-05 | 1996-02-27 | Nancy L. Parenteau | Cell culture systems and media |
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2004
- 2004-03-11 JP JP2006507065A patent/JP2006520598A/ja active Pending
- 2004-03-11 US US10/797,952 patent/US7300794B2/en not_active Expired - Fee Related
- 2004-03-11 CA CA002518496A patent/CA2518496A1/en not_active Abandoned
- 2004-03-11 EP EP04719713A patent/EP1606395A4/en not_active Withdrawn
- 2004-03-11 WO PCT/US2004/007390 patent/WO2004083384A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP1606395A2 (en) | 2005-12-21 |
| US7300794B2 (en) | 2007-11-27 |
| US20040185560A1 (en) | 2004-09-23 |
| WO2004083384A2 (en) | 2004-09-30 |
| JP2006520598A (ja) | 2006-09-14 |
| EP1606395A4 (en) | 2006-06-14 |
| WO2004083384A3 (en) | 2005-10-20 |
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| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |