JP2006518185A - 改良された灌流インキュベーター - Google Patents
改良された灌流インキュベーター Download PDFInfo
- Publication number
- JP2006518185A JP2006518185A JP2004537807A JP2004537807A JP2006518185A JP 2006518185 A JP2006518185 A JP 2006518185A JP 2004537807 A JP2004537807 A JP 2004537807A JP 2004537807 A JP2004537807 A JP 2004537807A JP 2006518185 A JP2006518185 A JP 2006518185A
- Authority
- JP
- Japan
- Prior art keywords
- well
- medium
- perfusion
- incubator
- perfusion incubator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000010412 perfusion Effects 0.000 title claims abstract description 42
- 239000000463 material Substances 0.000 claims abstract description 22
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 17
- 239000002609 medium Substances 0.000 claims description 124
- 239000001963 growth medium Substances 0.000 claims description 16
- 239000012530 fluid Substances 0.000 claims description 7
- 229920002379 silicone rubber Polymers 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 4
- 239000012780 transparent material Substances 0.000 claims description 4
- 230000000712 assembly Effects 0.000 claims description 2
- 238000000429 assembly Methods 0.000 claims description 2
- 239000012531 culture fluid Substances 0.000 claims description 2
- 230000035699 permeability Effects 0.000 claims 1
- 239000007789 gas Substances 0.000 abstract description 53
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 20
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 10
- 239000001569 carbon dioxide Substances 0.000 abstract description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 9
- 239000001301 oxygen Substances 0.000 abstract description 9
- 229910052760 oxygen Inorganic materials 0.000 abstract description 9
- 210000002257 embryonic structure Anatomy 0.000 abstract description 4
- 238000011534 incubation Methods 0.000 abstract 2
- 210000001161 mammalian embryo Anatomy 0.000 description 28
- 210000004027 cell Anatomy 0.000 description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 238000010438 heat treatment Methods 0.000 description 8
- 239000003102 growth factor Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 229920001296 polysiloxane Polymers 0.000 description 5
- 239000002699 waste material Substances 0.000 description 5
- 230000012010 growth Effects 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 3
- 230000003750 conditioning effect Effects 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 239000004417 polycarbonate Substances 0.000 description 3
- 229920000515 polycarbonate Polymers 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- -1 for example Polymers 0.000 description 2
- 230000007773 growth pattern Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000010979 pH adjustment Methods 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 208000009701 Embryo Loss Diseases 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000002459 blastocyst Anatomy 0.000 description 1
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 230000008011 embryonic death Effects 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000007320 rich medium Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2002951423A AU2002951423A0 (en) | 2002-09-17 | 2002-09-17 | Improved perfusion incubator |
| US41242302A | 2002-09-21 | 2002-09-21 | |
| PCT/US2003/028930 WO2004027016A1 (en) | 2002-09-17 | 2003-09-15 | Improved perfusion incubator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006518185A true JP2006518185A (ja) | 2006-08-10 |
| JP2006518185A5 JP2006518185A5 (https=) | 2006-10-12 |
Family
ID=36968214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004537807A Pending JP2006518185A (ja) | 2002-09-17 | 2003-09-15 | 改良された灌流インキュベーター |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2006518185A (https=) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006011130A (ja) * | 2004-06-28 | 2006-01-12 | Asahi Kasei Aimii Kk | コンタクトレンズ |
| JP2008092811A (ja) * | 2006-10-06 | 2008-04-24 | Hitachi Medical Corp | 細胞培養装置 |
-
2003
- 2003-09-15 JP JP2004537807A patent/JP2006518185A/ja active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006011130A (ja) * | 2004-06-28 | 2006-01-12 | Asahi Kasei Aimii Kk | コンタクトレンズ |
| JP2008092811A (ja) * | 2006-10-06 | 2008-04-24 | Hitachi Medical Corp | 細胞培養装置 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20060822 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20060822 |
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| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20091013 |
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| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20100309 |