JP2006304777A - Method for culturing highly functional plant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for obtaining crops such as plants and fruits containing vitamin C and polyphenol and carotenoid in large amounts and having antioxidation function without causing lowering of yield by enhancing functions which various plants which consumers eat have originally. <P>SOLUTION: When culturing a plant 3 by using a compound aqueous solution and/or a compound solid material containing, in a prescribed amount, a mixed trace element containing boron and manganese and iron and copper and/or at least one trace element selected from zinc, molybdenum and selenium, the compound aqueous solution is used directly or in a state diluted with water and the aqueous solution is applied to soil 1 or sprayed onto leaves 4 and the compound solid material is applied to soil 1 and the trace element is applied to the plant 3 to attain the object. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a method for cultivating highly functional plants, and more specifically, cultivating plants having an antioxidant function by containing a large amount of vitamin C, polyphenols and carotenoids by applying specific trace elements. It is about how to do.

  The present plant is inferior in its functional component content compared to the past (see Non-Patent Document 1). Several methods have been proposed to improve these to obtain plants with old functional ingredient content.

For example, it has been studied to perform gene recombination of plants to produce new varieties of highly functional plants such as crops rich in nutritional components and rich in anticancer effects and vaccine effects (see Non-Patent Document 2). .
Also, reduce the nitrogen content of plant-cultivated soil to eliminate ground strength, cut off water and nitrogen fertilizer after germination or planting, and if the plant begins to wither in such dredged areas, a small amount of about 1/10 to 1/100 of the usual A method of cultivating a high vitamin C plant by reducing water and fertilizer, such as giving water and nitrogen fertilizer has been proposed (see Patent Document 1).
Furthermore, a method for increasing the yield of plant polyphenols in which the polyphenol component in the plant body is increased by growing the polyphenol-producing plant under conditions loaded with stress such as light stress or water stress has been proposed (Patent Literature). 2).
Vitamins and minerals in vegetables, pages 10-11, Table 1-1 Comparison of “4th Japanese Food Standard Ingredients” and “5th Japanese Food Standard Ingredients” University Press http://www.biotech-house.jp/ (Agriculture, Forestry and Fisheries Advanced Technology Industry Promotion Center) JP 59-120027 A JP 2003-009665 A

  However, the genetic recombination method has safety problems and cost problems, and there are problems that require time to solve them. The method of cultivating high vitamin C plants by reducing fertilizer, light stress or water The method of increasing the yield of plant polyphenols grown under stressed conditions is laborious and has a problem of reduced yield of plants.

  The purpose of the present invention is to apply genetic recombination methods, fertilizer reduction methods, plant cultivation methods, light stress or water stress in order to contribute to human health through various plants that consumers eat. By using the method of applying trace elements without using the method of growing under the conditions, the function that the plant should have originally is enhanced as in the past, so that it contains vitamin C, polyphenols and carotenoids in a large amount and is antioxidant It is to provide a method for cultivating a plant having a function and capable of taking or supplementing vitamin C, polyphenol and carotenoid as supplements.

  The method for cultivating a highly functional plant according to claim 1 of the present invention for solving the above-mentioned problem is a mixed trace element containing boron and manganese and iron and copper and / or at least one selected from zinc, molybdenum and selenium. When cultivating a plant using a compound aqueous solution containing a predetermined amount of a trace element and / or the compound solid, the compound aqueous solution is irrigated or foliarly applied to the soil as it is or diluted with water. The compound solids are applied to the soil and the trace element is applied to the plant so that it contains a higher amount of vitamin C and polyphenols and carotenoids than when the trace element is not applied. And obtaining a plant having an antioxidant function.

  The method for cultivating a highly functional plant according to claim 2 of the present invention is the method for cultivating a highly functional plant according to claim 1, wherein the compound solid is applied to the soil before planting the plant, and the plant is applied after application. The plant is planted or applied to the soil where the plant is growing.

  The method for cultivating a highly functional plant according to claim 3 of the present invention is the method for cultivating a highly functional plant according to claim 1 or 2, wherein the compound aqueous liquid and / or the compound solid is mixed with a fertilizer component. It is characterized by using.

  The method for cultivating a highly functional plant according to claim 4 of the present invention is the method for cultivating a highly functional plant according to any one of claims 1 to 3, wherein the trace element in the soil where the plant is cultivated is used. Analyzing the results, comparing the analysis result with a predetermined optimum value of the trace element, and using a predetermined amount of the compound solid prepared by mixing the trace element that is deficient to the optimum value. .

  According to the method for cultivating highly functional plants according to claim 1 of the present invention, the functions that should be inherently possessed by various plants eaten by consumers are enhanced, and contain a large amount of vitamin C, polyphenol and carotenoid, and Plants having an antioxidant function can be obtained, and the harvested products such as plants and fruits have a remarkable effect that vitamin C, polyphenols and carotenoids can be ingested or supplemented as supplements.

  According to the method for cultivating a highly functional plant according to claim 2 of the present invention, a plant plant or a growing plant gradually and reliably absorbs trace elements in the compound solid matter applied to the soil, and vitamin C Furthermore, there is a further remarkable effect that a harvested product such as a plant or fruit containing a larger amount of polyphenol and carotenoid can be surely harvested.

  According to the method for cultivating a highly functional plant according to claim 3 of the present invention, the plant absorbs a fertilizer component, absorbs a trace element while growing, and a plant or fruit containing a larger amount of vitamin C, polyphenol, and carotenoid There is a further remarkable effect that the harvest can be harvested more reliably.

  According to the method for cultivating a highly functional plant according to claim 4 of the present invention, it is possible to reliably harvest a crop such as a plant or fruit containing a larger amount of vitamin C and polyphenols and carotenoids according to the soil in which the plant is cultivated. There is a further remarkable effect.

Next, the contents of the present invention will be described in detail.
1A to 1E are diagrams schematically illustrating an example of a method for cultivating a highly functional plant using the trace element of the present invention.
As shown in FIG. 1A, first, the trace element in the soil 1 where the plant is grown is analyzed. Then, the analysis result is compared with a predetermined optimum value of the trace element, and the compound aqueous liquid and the compound solid material prepared so that the insufficient trace element is set to the optimum value are prepared.
Then, as shown in (b), a predetermined amount of the prepared compound solid is applied to the soil before planting the plant to obtain soil 2 in which the trace element is the optimum value. Under the present circumstances, the said compound solid substance can also be used with a fertilizer component.
Thereafter, as shown in (c), the plant 3 is sown or planted in the soil 2 to which the compound solid is applied.
As shown in (d), the compound aqueous liquid is sprayed on the leaf surface 4 of the plant 3 by a predetermined amount, and the soil 2 is irrigated as necessary. Under the present circumstances, the said compound aqueous liquid and the water to be used can also be used with a fertilizer component.
And, as shown in (e), by cultivating the plant 3 in this way, the function inherent to the plant 3 is enhanced, the plant contains a large amount of vitamin C, polyphenol and carotenoid, and has an antioxidant function. 3 can be harvested.

2 (a) to 2 (d) are diagrams for schematically explaining examples of other cultivation methods for highly functional plants using the trace elements of the present invention.
As shown in FIG. 2 (a), the trace elements in the soil 1A in the field where fruit trees such as peaches and plants 3A such as tea are planted are analyzed for many years. Then, the analysis result is compared with a predetermined optimum value of the trace element, and the compound aqueous liquid and the compound solid material prepared so that the insufficient trace element is set to the optimum value are prepared.
Then, as shown in (b), a predetermined amount of the compound solid prepared at the plant stock of the plant 3A, between the trees, etc. is applied to obtain soil 2A in which the trace element is the optimum value. Under the present circumstances, the said compound solid substance can also be used with a fertilizer component.
Thereafter, as shown in (c), the compound aqueous liquid is sprayed on the leaf surface 4A of the plant 3A by a predetermined amount as necessary, and the soil 2 is irrigated as necessary. Under the present circumstances, the said compound aqueous liquid and the water to be used can also be used with a fertilizer component.
Then, as shown in (d), by cultivating the plant 3A in this way, the functions inherent to the plant 3A are enhanced, and a peach having an antioxidant function, containing a large amount of vitamin C, polyphenol and carotenoid. Harvested products 5 such as fruits and tea can be harvested.

In the present invention, the mechanism of action by which trace elements improve the function of plants has not been elucidated, but it is clear that trace elements in soil in recent years have decreased from the past, and this lack is apparent. By replenishing the trace elements, it is possible to restore the function as in the past, and to grow a plant containing an increased amount of vitamin C, polyphenol and carotenoid and having an antioxidant function.
Carotenoids are yellow-orange-red components such as carrots, tomatoes, strawberries, mangoes and other vegetables and fruits. Carotenoids are also present in the chloroplast and protect chlorophyll from light destruction.
It is known that carotenoids have different types and compositions depending on plants.
Major carotenoids include α-carotene (mainly included in carrots, pumpkins, oranges, etc.), β-carotene (mainly included in carrots, sweet potatoes, pumpkins, greenish yellow vegetables, etc.), γ-carotene (mainly apricots) ), Lycopene (mainly included in tomatoes, watermelons, etc.), lutin (mainly included in oranges, pumpkins, kale, spinach, etc.), cryptoxanthin (mainly peppers red, strawberries, corn, oranges, etc.) ) And capsanthin (mainly contained in Karako, etc.).
Among carotenoids, α-carotene, β-carotene, γ-carotene, cryptoxanthine and the like are converted into retinol (vitamin A) via retinal in the human intestinal wall.
Carotenoids themselves are known to have antioxidant properties (reactive oxygen scavenging action), immunostimulatory action, anticancer action, and the like.

The trace element used in the present invention is one of a mixed trace element containing boron and manganese and iron and copper and at least one trace element selected from zinc, molybdenum and selenium, and the other is zinc, molybdenum and selenium. At least one trace element selected from
Chlorine can also be used as a trace element.

  The compound containing these trace elements used in the present invention may be an inorganic compound or an organic compound, or a mixture thereof, or a water-soluble compound, a poorly water-soluble compound, or a water-soluble compound. Even if it is a compound without nature, or even if it is a mixture of two or more of these, it can be used.

  The compound aqueous solution containing these trace elements used in the present invention may be an aqueous solution in which the compound is dissolved in water, or the compound is dispersed, suspended or emulsified in water. It may be present, or a mixture of two or more of these, any of which can be used.

  As the compound solid containing these trace elements used in the present invention, the compound is in a solid dry state and may or may not contain water, and the water content is particularly limited. It is not a thing. The shape is not particularly limited. Granules such as granules, tablets, packs, powders and capsules can be preferably used because they are easy to apply and easy to handle. A solid material of a water-soluble compound, a hardly water-soluble compound, a water-insoluble compound, or a mixture of two or more thereof can be used.

  When water is applied to the soil or sprayed on the foliage, it is preferable to use an aqueous solution in which the compound is dissolved in water from the viewpoint of easy intake by plants and easy handling.

  When applying by mixing with soil, it is preferable to use a compound solid containing a trace element. The compound to be used is preferably a water-soluble compound because it can be easily ingested by plants, but it may be a poorly water-soluble compound or a non-water-soluble compound, or a solid of two or more of these compounds. Can be used.

  For example, a poorly water-soluble compound or a compound that is not water-soluble can be easily taken in by the action of microorganisms in the soil, dissolved by the root acid produced by the plant and easily used by plants, or dissolved by fertilizer components. And can be used in the present invention because it is incorporated into plants together with fertilizer components. However, the reason why these compounds can be used in the present invention is not limited thereto.

In the present invention, a plant is cultivated using a compound aqueous liquid containing a predetermined amount of these trace elements, using a compound solid, or using both in combination.
Analyzing the content of trace elements in the soil where plants are cultivated, and comparing the analysis results with the optimum values of the trace elements determined in advance, the compound aqueous liquid prepared so that the missing trace elements become the optimum values It is preferable to use a predetermined amount of the compound solid. By cultivating the plant in this manner, it is possible to efficiently and reliably harvest a plant that contains a larger amount of vitamin C, polyphenol, and carotenoid and has an antioxidant function. Prevents or suppresses damage such as burning roots or falling white, and there are too many trace elements left in the soil, and the remaining trace elements run out by rainwater, etc., polluting the environment. Can be prevented / suppressed.

The optimum value of the trace element can be determined by combining various test values and experience values. An example of the optimum value of the trace element will be described next.
Optimum value (mass ppm in soil):
Boron 0.4-3.5
Manganese 7-35
Iron 10-150
Copper 1-5
Zinc 10-40
Molybdenum 0.09-6
Selenium 0.1-1

  In this invention, it is preferable to apply the said compound solid substance to the soil before the planting of a plant, and sow | plant or plant a plant after application. Plants that have been sown or planted can gradually and reliably absorb trace elements in the compound solids applied to the soil to reliably harvest plants that contain higher amounts of vitamin C and polyphenols and carotenoids.

In the present invention, the compound aqueous liquid and / or the compound solid may be mixed with a nitrogen source such as nitrate, a phosphate source, potassium salt, bitter earth, lime and the like fertilizer components that are effective for plant nutrition. It can be performed as necessary.
Also in the case of fertilizer components to be mixed, it is preferable to determine the application rate based on the result of soil analysis.

The fertilizer component used together with the trace element in the present invention is a fertilizer including at least one of the second three elements of nitrogen, phosphoric acid, at least one of the three elements of lime, lime, bitter earth, and silicic acid. It is a fertilizer component containing components and other sulfur, and a multi-fertilizer component containing nitrogen, phosphoric acid, and potassium can be preferably used. Specific examples of the fertilizer component include the following.
For example, compost, compost, livestock manure, human manure, grass ash, wood ash, rice straw, straw, straw, rice bran, wheat straw, soybean pod, nitrogenous fertilizer, phosphate fertilizer, sorghum fertilizer, compound fertilizer, calcareous Fertilizer, siliceous fertilizer, clay soil fertilizer, manganese fertilizer, boron fertilizer, trace element compound fertilizer, organic fertilizer, fish meal, livestock and poultry manure, livestock and poultry manure, livestock and poultry Feces burning ash, sludge fertilizer, sugar by-product lime, converter slag, shell stone powder, various agricultural products, food industry residue, fermentation industry waste, fermentation waste, textile industry waste, fishery industry Waste, sewage sludge, municipal waste compost, bone ash, etc. These fertilizer components may be used alone or in combination of two or more at any ratio.
In addition to these fertilizer components, it is also possible to mix soil improvers such as zeolite, bentonite, vermiculite, peat, pearlite, corrosive acid materials, charcoal, polyethyleneimine materials, and polyvinyl alcohol materials.

  In the present invention, surfactants such as anionic surfactants, nonionic surfactants, and cationic and amphoteric surfactants can be used in combination.

Hereinafter, although an example and a comparative example explain the present invention, the present invention is not limited at all by these examples.
In addition,% and ppm described in the following examples and comparative examples represent mass% and mass ppm, respectively.

Example 1
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, a compound solid adjusted to 0.5% boron, 3.5% manganese, 2.7% iron, 0.75% copper, 3.35% zinc, and 0.025% molybdenum was used.
The above-mentioned compound solid 40Kg / 10a was mixed with the soil before planting of Komatsuna (variety Hamami No. 2) and applied so that the trace element in the soil would be the optimum value.
Komatsuna is cultivated in a glass house, collected in the edible part of the food, and collected vitamin C content (mg / 100ml) by the following vitamin C measurement method, polyphenol measurement method, and antioxidant activity measurement method. ), Polyphenol content (μmol / ml) and antioxidant activity (μmol / ml) were measured, and the content (μg / 100 g) of β-carotene which is a typical carotenoid of Komatsuna was measured by the carotenoid measurement method. Shown in

(Vitamin C measurement method, polyphenol measurement method, antioxidant activity measurement method)
The plants were collected from the edible part, and the three strains were combined into one treatment group, and vitamin C content, polyphenol content and antioxidant activity were measured in 5 repetitions.
An extract extracted with 5% metaphosphoric acid was prepared, and the vitamin C content was measured using the extract as it was. Vitamin C was colorimetrically determined by the hydrazine method [new food analysis method, edited by Japan Food Science and Technology Society, new food analysis method editorial committee, publisher: Kozo Co., Ltd., issue date: November 30, 1996, page 444, 4-8 vitamin C quantification method, pages 444-447, [Example 3] According to the quantification of AsA by the hydrazine method].

  A part of the extract for vitamin C measurement was subjected to pH adjustment and EtOH concentration adjustment using 1N NaOH, distilled water and 100% EtOH, and then the total polyphenol content and DPPH radical scavenging activity were measured.

  Polyphenol content was determined colorimetrically using gallic acid as a standard substance by the Folin-Dennis method. [Food Functional Research Method, Editor: Kazuaki Shinohara, etc., Publisher: Korin Co., Ltd., Date of issue: 2000] May 10, pp. 318-322,4. Separation and structure determination of functional food components, 4-1 According to polyphenol analysis method (colorimetric method, HPLC analysis method)].

  DPPH radical scavenging activity was determined by measuring the degree of fading of DPPH by the extract using a microplate reader and converting it to the equivalent of Trolox [Food Functional Research Method, Editor: Kazuaki Shinohara, etc., Publisher: (Stocks) ) Korin, Issue Date: May 10, 2000, pp. 218-220, 3-3-9 Antioxidant Function, (1) Evaluation of Spectroscopic Antioxidant Function, According to measurement of DPPH radical scavenging ability with a spectrophotometer].

(Carotenoid measurement method)
The plants were collected from the edible part, and typical carotenoids contained in the plants were measured.
Prepare an extract using ethanol: tert-butyl methyl ether (1: 1) added with BHA (100 mg / l), separate the extract using high performance liquid chromatography, UV detection Measured with a vessel. [New Food Analysis Method, edited by Japan Society for Food Science and Technology, New Food Analysis Method Editorial Committee, Publisher: Kozo Co., Ltd., Date of issue: November 30, 1996, page 316, 3 -4 β-carotene, pp. 322 to 325, according to high performance liquid chromatography method].

(Comparative Example 1)
Komatsuna (variety Hamami No. 2) was cultivated in the same manner as in Example 1 except that no trace elements were applied, and vitamin C content, polyphenol content, antioxidant activity, carotenoid (β-carotene) content were measured, and Table 1 Shown in

  As shown in Table 1, when trace elements were applied to soil (Example 1), the vitamin C content, polyphenol content, and carotenoid (β-carotene) content of Komatsuna improved compared to the non-application of Comparative Example 1. In addition, it can be seen that the antioxidant activity is improved.

(Example 2)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 3 ppm boron, 20 ppm manganese, 20 ppm iron, 3 ppm copper, 24 ppm zinc, and 1 ppm molybdenum was used.
The trace amount of aqueous liquid prepared in this way was sprayed on Komatsuna (variety Hamami No. 2) three times at intervals of 7 days.
Komatsuna is cultivated in a glass house, the edible portion is collected 9 days after the last foliar application, and the vitamin C content, polyphenol content, antioxidant activity, and carotenoid (β-carotene) content are measured in the same manner as in Example 1. The results are shown in Table 1.

  As shown in Table 1, when trace elements were sprayed on the foliage (Example 2), the vitamin C content, polyphenol content, and carotenoid (β-carotene) content of Komatsuna improved compared to the non-application of Comparative Example 1. It can also be seen that the antioxidant activity was improved.

(Example 3)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 7.5 ppm boron, 50 ppm manganese, 50 ppm iron, 7.5 ppm copper, 60 ppm zinc, and 2.5 ppm molybdenum was used.
The trace amount of aqueous liquid prepared in this manner was applied to Komatsuna (variety Hamami 2) three times at intervals of 7 days.
Komatsuna is cultivated in a glass house, the edible portion is collected 9 days after the last irrigation application, and the vitamin C content, polyphenol content, antioxidant activity, carotenoid (β-carotene) content are measured in the same manner as in Example 1. The results are shown in Table 1.

  As shown in Table 1, when the trace element was applied with water (Example 3), the vitamin C content, polyphenol content, and carotenoid (β-carotene) content of Komatsuna improved compared to the non-application of Comparative Example 1. It can also be seen that the antioxidant activity was improved.

Example 4
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, a compound solid adjusted to 0.5% boron, 3.5% manganese, 2.7% iron, 0.75% copper, 3.35% zinc, and 0.025% molybdenum was used.
40 kg / 10a of the above compound solid was mixed with the soil before planting spinach (variety active) and applied so that the trace elements in the soil reached the optimum values.
Komatsuna is cultivated in a glass house, collected the edible part of the edible size, and in the same manner as in Example 1, the vitamin C content, polyphenol content, antioxidant activity, carotenoid (β-carotene) content. The results are shown in Table 1.

(Comparative Example 2)
Spinach (variety active) was cultivated in the same manner as in Example 4 except that no trace elements were applied, and vitamin C content, polyphenol content, antioxidant activity, and carotenoid (β-carotene) content were measured in the same manner as in Example 1. The results are shown in Table 1.

  As shown in Table 1, when trace elements were applied to the soil (Example 4), the spinach vitamin C content, polyphenol content, and carotenoid (β-carotene) content were improved compared to the non-application of Comparative Example 2. It can also be seen that the antioxidant activity was improved.

(Example 5)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 7.5 ppm boron, 50 ppm manganese, 50 ppm iron, 7.5 ppm copper, 60 ppm zinc, and 2.5 ppm molybdenum was used.
The microelement aqueous solution thus prepared was irrigated three times at intervals of 7 days to spinach (variety active).
Spinach is cultivated in a glass house, the edible portion is collected 18 days after the last irrigation application, and the vitamin C content, polyphenol content, antioxidant activity, carotenoid (β-carotene) content are measured in the same manner as in Example 1. The results are shown in Table 1.

  As shown in Table 1, when the trace element was applied with water (Example 5), the vitamin C content of spinach, the polyphenol content, and the carotenoid (β-carotene) content were improved compared to the non-application of Comparative Example 2. It can also be seen that the antioxidant activity was improved.

(Example 6)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, a compound solid adjusted to 0.6% boron, 3% manganese, 4.1% iron, 0.5% copper, 2.7% zinc, and 0.01% molybdenum was used.
90 kg / 10a of the above-mentioned compound solid was mixed with the soil before planting of turnip (variety Colorful Live) and applied so that the trace elements in the soil reached the optimum values.
The turnip was cultivated in a glass house, and the edible portion of the edible portion was collected and the vitamin C content and polyphenol content were measured in the same manner as in Example 1. The fresh weight of the edible part was also measured. The results are shown in Table 2.
The carotenoid content was not measured.

(Comparative Example 3)
A turnip (variety colorful live) was cultivated in the same manner as in Example 6 except that no trace element was applied, and the vitamin C content and polyphenol content were measured in the same manner as in Example 1. The fresh weight of the edible portion was also measured. It was measured. The results are shown in Table 2.
The carotenoid content was not measured.

  As shown in Table 2, when trace elements were applied to soil (Example 6), the vitamin C content and polyphenol content of the turnip were improved without reducing the yield, compared to the non-application of Comparative Example 3. I understand.

(Example 7)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 3 ppm boron, 20 ppm manganese, 20 ppm iron, 3 ppm copper, 24 ppm zinc, and 1 ppm molybdenum was used.
The trace amount of the aqueous liquid prepared in this manner was sprayed onto the turnip (variety colorful live) three times at intervals of 7 days.
Turnips were cultivated in a glass house, and edible parts were collected 7 days after the last foliar application, and vitamin C content and polyphenol content were measured in the same manner as in Example 1. In addition, the fresh weight of the edible portion was also measured, and the results are shown in Table 2.
The carotenoid content was not measured.

  As shown in Table 2, when the trace elements were sprayed on the foliage (Example 7), the vitamin C content and polyphenol content of the turnip could be improved without reducing the yield compared to the non-application of Comparative Example 3. I understand.

(Example 8)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 7.5 ppm boron, 50 ppm manganese, 50 ppm iron, 7.5 ppm copper, 60 ppm zinc, and 2.5 ppm molybdenum was used.
The microelement aqueous solution thus prepared was irrigated 3 times at intervals of 7 days to turnips (variety Colorful Live).
Turnips were cultivated in a glass house, and edible parts were collected 7 days after the last irrigation application, and the vitamin C content and polyphenol content were measured in the same manner as in Example 1. In addition, the fresh weight of the edible portion was also measured, and the results are shown in Table 2.
The carotenoid content was not measured.

  As shown in Table 2, when the trace element was irrigated (Example 8), the vitamin C content and polyphenol content of the turnip could be improved without lowering the yield compared to the non-application of Comparative Example 3. I understand.

Example 9
As the soil for cultivation, a soil containing almost no trace elements was used.
A water-soluble compound aqueous solution diluted with a water-soluble compound (sodium selenate) so that sodium selenate was 1 ppm was used.
The trace amount of the aqueous liquid prepared in this way was sprayed on the tomato (variety Toko K) once.
Tomato was cultivated in a glass house, and the ground part was collected 6 days after foliar application, and the vitamin C content and polyphenol content were measured in the same manner as in Example 1. The results are shown in Table 3.
The carotenoid content was not measured.

(Comparative Example 4)
A tomato (variety Toko K) was cultivated in the same manner as in Example 9 except that no trace element was applied, and the vitamin C content and polyphenol content were measured in the same manner as in Example 1. The results are shown in Table 3.
The carotenoid content was not measured.

  As shown in Table 3, it can be seen that when the trace element was sprayed on the leaves (Example 9), the vitamin C content and polyphenol content of tomato were improved as compared with the non-application of Comparative Example 4.

(Example 10)
As the soil for cultivation, a soil containing almost no trace elements was used.
An aqueous solution of a water-soluble compound diluted with a water-soluble compound (sodium selenate) so that sodium selenate was 0.1 ppm was used.
The water solution of the trace element thus prepared was irrigated once to tomato (variety Toko K).
Tomatoes were cultivated in a glass house, the ground part was collected 6 days after the irrigation treatment, 3 strains were combined into one treatment zone, and vitamin C content and polyphenol content were measured in the same manner as in Example 1. The results are shown in Table 3.
The carotenoid content was not measured.

(Comparative Example 5)
A tomato (variety Toko K) was cultivated in the same manner as in Example 10 except that no trace element was applied, and the vitamin C content and polyphenol content were measured in the same manner as in Example 1. The results are shown in Table 3.
The carotenoid content was not measured.

  As shown in Table 3, it can be seen that when the trace element was irrigated (Example 10), the vitamin C content and polyphenol content of tomato were improved as compared with the non-application of Comparative Example 5.

(Example 11)
As the soil for cultivation, a soil containing almost no trace elements was used.
The compound aqueous liquid adjusted so that a zinc compound might be 24 ppm as a trace element was used.
The microelement aqueous solution thus prepared was irrigated once in Komatsuna (variety Hamami No. 2).
Komatsuna is cultivated in a glass house, 8 days after irrigation treatment, the edible portion is collected, 3 strains are combined into one treatment zone, and vitamin C content and polyphenol content are measured in the same manner as in Example 1 in 3 iterations. did. The results are shown in Table 4.
The carotenoid content was not measured.

(Comparative Example 6)
Komatsuna (variety Hamami No. 2) was cultivated in the same manner as in Example 11 except that the trace element was not applied, and the vitamin C content and polyphenol content were measured in the same manner as in Example 1. The results are shown in Table 4.
The carotenoid content was not measured.

  As shown in Table 4, it can be seen that when the trace element was irrigated (Example 11), the vitamin C content and polyphenol content of Komatsuna were improved as compared with the non-application of Comparative Example 6.

(Example 12)
As the soil for cultivation, an aqueous solution containing almost no trace element was used, and an aqueous compound liquid prepared so that the molybdenum compound was 1 ppm as the trace element was used.
The microelement aqueous solution thus prepared was irrigated once in Komatsuna (variety Hamami No. 2).
Komatsuna is cultivated in a glass house, 8 days after irrigation treatment, the edible portion is collected, 3 strains are combined into one treatment zone, and vitamin C content and polyphenol content are measured in the same manner as in Example 1 in 3 iterations. did. The results are shown in Table 4.
The carotenoid content was not measured.

  As shown in Table 4, it can be seen that when the trace element was irrigated (Example 12), the vitamin C content and polyphenol content of Komatsuna were improved as compared with the non-application of Comparative Example 6.

(Example 13)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, a compound solid adjusted to 0.6% boron, 3% manganese, 4.1% iron, 0.5% copper, 2.7% zinc, and 0.01% molybdenum was used.
The above-mentioned compound solid 90Kg / 10a was mixed with the soil before planting Komatsuna (variety irradiance) and applied so that the trace element in the soil would be the optimum value.
Komatsuna is cultivated in a glass house, collected the edible part of the edible, collected by the above carotenoid measurement method, the content of β-carotene, which is a typical carotenoid of Komatsuna (μg / 100g) The fresh weight (g) of the edible part was also measured, and the results are shown in Table 5.
In addition, the vitamin C content, polyphenol content, and antioxidant activity were not measured.

(Comparative Example 7)
Komatsuna (variety iris) was cultivated in the same manner as in Example 13 except that no trace elements were applied, the carotenoid (β-carotene) content was measured, and the fresh weight (g) of the edible portion was also measured. Is shown in Table 5.
In addition, the vitamin C content, polyphenol content, and antioxidant activity were not measured.

  As shown in Table 5, when the trace element was applied to the soil (Example 13), the carotenoid (β-carotene) content was improved without reducing the yield as compared to the non-application of Comparative Example 7. I understand.

(Example 14)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 3 ppm boron, 20 ppm manganese, 20 ppm iron, 3 ppm copper, 24 ppm zinc, and 1 ppm molybdenum was used.
The trace amount of the aqueous liquid prepared in this way was sprayed once on Komatsuna (variety irradiance).
Komatsuna is cultivated in a glass house, 12 days after the last foliar application, the edible portion is collected and the carotenoid (β-carotene) content is measured in the same manner as in Example 13, and the fresh weight (g) of the edible portion is also measured. The results are shown in Table 5.
In addition, the vitamin C content, polyphenol content, and antioxidant activity were not measured.

  As shown in Table 5, the amount of carotenoid (β-carotene) was improved without reducing the yield of Komatsuna, compared to the non-application of Comparative Example 7 when the trace elements were sprayed on the foliage (Example 14). I understand.

(Example 15)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 7.5 ppm boron, 50 ppm manganese, 50 ppm iron, 7.5 ppm copper, 60 ppm zinc, and 2.5 ppm molybdenum was used.
The microelement aqueous solution prepared in this manner was irrigated once to Komatsuna (variety erisai).
Komatsuna is cultivated in a glass house, the edible part is collected 12 days after the last irrigation application, the carotenoid (β-carotene) content is measured as in Example 13, and the fresh weight (g) of the edible part is also measured. The results are shown in Table 5.
In addition, the vitamin C content, polyphenol content, and antioxidant activity were not measured.

  As shown in Table 5, it can be seen that when the trace element was irrigated (Example 15), the carotenoid (β-carotene) content was improved without lowering the yield as compared to the non-application of Comparative Example 7.

(Example 16)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, a compound solid adjusted to 0.6% boron, 3% manganese, 4.1% iron, 0.5% copper, 2.7% zinc, and 0.01% molybdenum was used.
The above-mentioned compound solid 90Kg / 10a was mixed with the soil before planting carrots (variety baby piccolo) and applied so that the trace elements in the soil reached the optimum values.
Carrots are cultivated in a glass house, collected in the edible portion of food, and collected in the edible part. Vitamin C content (mg / 100ml) by the vitamin C measurement method, polyphenol measurement method, and carotenoid measurement method described above , Polyphenol content (μmol / ml), α-carotene content (μg / 100 g) and β-carotene (μg / 100 g) for α-carotene and β-carotene, which are typical carotenoids of carrots, Table 6 shows.

(Comparative Example 8)
Carrot (variety baby piccolo) was cultivated in the same manner as in Example 16 except that no trace element was applied, and vitamin C content, polyphenol content, and carotenoid (α-carotene, β-carotene) content were measured. It is shown in FIG.

  As shown in Table 6, when a trace element was applied to soil (Example 16), the vitamin C content, polyphenol content, carotenoid (α-carotene, β-carotene) content compared to no application of Comparative Example 8 It turns out that improved.

(Example 17)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 3 ppm boron, 20 ppm manganese, 20 ppm iron, 3 ppm copper, 24 ppm zinc, and 1 ppm molybdenum was used.
The trace amount of aqueous liquid prepared in this manner was sprayed on carrots (variety baby piccolo) five times at intervals of 7 days.
Carrots are cultivated in a glass house, edible parts are collected 7 days after the last foliar application, and vitamin C content, polyphenol content and carotenoid (α-carotene, β-carotene) content are measured in the same manner as in Example 16. The results are shown in Table 6.

  As shown in Table 6, when the trace elements were sprayed on the foliage (Example 17), the vitamin C content, the polyphenol content, and the carotenoid (α-carotene, β-carotene) content compared to the non-application of Comparative Example 8 It turns out that it improved.

(Example 18)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 7.5 ppm boron, 50 ppm manganese, 50 ppm iron, 7.5 ppm copper, 60 ppm zinc, and 2.5 ppm molybdenum was used.
The microelement aqueous solution prepared in this manner was applied to carrots (variety baby piccolo) by irrigation 5 times at intervals of 7 days.
Carrots are cultivated in a glass house, 12 days after the last irrigation application, the edible portion is collected and the vitamin C content, polyphenol content, carotenoid (α-carotene, β-carotene) content is measured in the same manner as in Example 16. The results are shown in Table 6.

  As shown in Table 6, when the trace element was applied with irrigation (Example 18), the vitamin C content, polyphenol content, and carotenoid (α-carotene, β-carotene) content improved compared to the non-application of Comparative Example 8. You can see that

(Example 19)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, a compound solid adjusted to 0.6% boron, 3% manganese, 4.1% iron, 0.5% copper, 2.7% zinc, and 0.01% molybdenum was used.
The above-mentioned compound solid 90Kg / 10a was mixed with soil before planting cherry tomatoes (variety coco) and applied so that trace elements in the soil reached the optimum values.
Mini tomatoes are cultivated in a glass house, collected from the fruit, and collected for food. Vitamin C content (mg / 100ml) by the above-mentioned vitamin C measurement method and carotenoid measurement method, typical carotenoid of tomato fruit Β-carotene and lycopene, β-carotene content (μg / 100 g) and lycopene content (μg / 100 g) were measured and are shown in Table 7. Polyphenol was not measured.

(Comparative Example 9)
Except for not applying the trace element, cherry tomatoes (variety coco) were cultivated in the same manner as in Example 19, and vitamin C content and carotenoid (β-carotene, lycopene) content were measured.
Polyphenol was not measured.

  As shown in Table 7, when the trace element was applied to soil (Example 19), the vitamin C content and carotenoid (β-carotene, lycopene) content were improved as compared to the non-application of Comparative Example 9. I understand.

(Example 20)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 3 ppm boron, 20 ppm manganese, 20 ppm iron, 3 ppm copper, 24 ppm zinc, and 1 ppm molybdenum was used.
The microelement aqueous solution thus prepared was sprayed onto cherry tomatoes (variety Coco) 6 times at intervals of 7 days.
The cherry tomatoes were cultivated in a glass house, the fruits were collected 10 days after the last foliar application, and the vitamin C content and carotenoid (β-carotene, lycopene) content were measured in the same manner as in Example 19. Table 7 shows the results. Shown in Polyphenol was not measured.

  As shown in Table 7, when the trace elements were sprayed on the foliage (Example 20), it was found that the vitamin C content and carotenoid (β-carotene, lycopene) content were improved as compared with the non-application of Comparative Example 9. .

(Example 21)
As the soil for cultivation, a soil containing almost no trace elements was used.
As a trace element, an aqueous compound liquid adjusted to 7.5 ppm boron, 50 ppm manganese, 50 ppm iron, 7.5 ppm copper, 60 ppm zinc, and 2.5 ppm molybdenum was used.
The aqueous solution of the trace element thus prepared was irrigated nine times at intervals of 7 days to cherry tomatoes (variety Coco).
Cherry tomatoes were grown in a glass house, and fruits were collected 14 days after the last irrigation application. The vitamin C content and carotenoid (β-carotene, lycopene) content were measured in the same manner as in Example 19, and the results are shown in Table 7. Show. Polyphenol was not measured.

(Comparative Example 10)
A cherry tomato (variety coco) was cultivated in the same manner as in Example 21 except that the trace element was not applied, and the vitamin C content and carotenoid (β-carotene, lycopene) content were measured.
Polyphenol was not measured.

  As shown in Table 7, it can be seen that when the trace element was irrigated (Example 21), the vitamin C content and carotenoid (β-carotene, lycopene) content were improved as compared with the non-application of Comparative Example 10.

  Without using a genetic recombination method with cost and safety problems, and using a method of cultivating a plant by reducing fertilizer, which is troublesome and causes a decrease in yield, or a method of stressing the plant In addition, according to the cultivation method of the present invention, functions that should be inherently possessed by various plants eaten by consumers are increased, and vitamin C and polyphenols and carotenoids are increased in a large amount without causing a decrease in yield. It is possible to obtain harvests such as plants and fruits that contain and have an antioxidant function, and the harvests such as plants and fruits that can be obtained and supplemented with vitamin C, polyphenols and carotenoids as supplements Since it has a remarkable effect, it has high industrial utility value.

(A)-(e) is a figure which illustrates typically an example of the cultivation method of the highly functional plant using the trace element of this invention. (A)-(d) is a figure which illustrates typically the example of the other cultivation method of the highly functional plant using the trace element of this invention.

Explanation of symbols

1, 1A Soil 2, 2A Soil 3, 3A Plant 4, 4A Leaf surface 5

Claims (4)

  When cultivating a plant using a mixed aqueous solution containing boron and manganese and iron and copper and / or a compound aqueous liquid containing a predetermined amount of at least one trace element selected from zinc, molybdenum and selenium and / or the compound solid In addition, the compound aqueous solution is used as it is or diluted with water, and then applied to the soil by irrigation or foliar application, and the compound solid is applied to the soil and the trace element is applied to the plant. By the above, a method for cultivating a highly functional plant, characterized in that a plant having an antioxidant function and containing a larger amount of vitamin C, polyphenol and carotenoid as compared with the case where the trace element is not applied is obtained.   The high-functional plant according to claim 1, wherein the compound solid is applied to the soil before planting the plant, and after the planting, the plant is planted or applied to the soil where the plant is growing. Cultivation method.   The method for cultivating a highly functional plant according to claim 1 or 2, wherein the compound aqueous liquid and / or the compound solid is used together with a fertilizer component.   Analyzing the trace elements in the soil for cultivating the plant, comparing the analysis result with a predetermined optimum value of the trace elements, and formulating the missing trace elements to the optimum value The method for cultivating a highly functional plant according to any one of claims 1 to 3, wherein a predetermined amount of solid is used.

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