JP2006298835A - Hairdressing agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a hairdressing agent excellent in hairdressing performance, containing practically neither ingredient blocking hair growth and hair fostering nor ingredient causing scalp stimulation, fallen hair, allergy and the like, and to provide a method for producing the agent. <P>SOLUTION: The hairdressing agent is obtained by containing extracts of microorganisms belonging to the genus Bacillus and/or a culture solution of the microorganisms as the active component. The method for producing the hairdressing agent comprises a step for cultivating the microorganism in a liquid nutrient medium, a step for pulverizing the microbe as it is and removing the microbe or the pulverized microbe, a step for collecting supernatant and, if required, a step for diluting with a cosmetically permissible medium. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a novel hair styling agent, and more particularly to a hair styling agent comprising a culture solution of a microorganism belonging to the genus Bacillus and / or an extract of the microorganism as an active ingredient, and a method for producing the same.

  Regardless of age and sex, hair styling is one of the appearances, and since ancient times, a wide variety of hair styling agents for hair styling have been widely used. Today, the demand for personal and commercial hairdressing agents is increasing. In conventional hairdressing agents, ingredients formulated for hairstyling prevent hair growth, hair growth, hair loss, allergies, etc. There were disadvantages that led to undesirable results. Examples of undesirable components in the prior art include mineral oils that hinder hair growth and hair growth, monohydric alcohols, dihydric alcohols, three alcohols that cause scalp irritation, hair loss, dryness, irritation, inflammation, and allergies. Examples include alcohols such as monohydric alcohols, polyhydric alcohols and fatty alcohols (excluding sugar alcohols), emulsifiers, gelling agents, foaming agents and the like. In view of this point, Patent Document 1 proposes a highly safe hair styling agent containing seawater as a natural ingredient and edible ingredients as active ingredients.

  Under such circumstances, the hair styling action is safe and substantially free of ingredients that hinder hair growth and hair growth, and ingredients that cause scalp irritation, hair loss, allergies, etc., which were disadvantages of conventional hair styling agents. The excellent hair styling agent has been crane-necked.

JP 2001-72550 A

  The present invention is a hair styling agent that is safe and excellent in hair styling, substantially free from ingredients that hinder hair growth and hair growth, and ingredients that cause scalp irritation, hair loss, drying, irritation, inflammation, allergies, etc. And its manufacturing method.

  As a result of earnest research focusing on natural ingredients having a hair-styling action for the purpose of solving the above-mentioned problems, the present inventors have achieved excellent hair-styling in a culture solution of microorganisms belonging to the genus Bacillus and / or an extract of the microorganisms. It was found that there is an effect. Furthermore, the culture medium and the extract effectively suppress the drying of the hair, and also find that the invention does not substantially cause scalp irritation, hair loss and allergy without hindering hair growth and hair growth. It came to be completed.

  That is, this invention solves the said subject by providing the hair-styling agent which contains the culture solution of the microorganisms which belong to the genus Bacillus, and / or the extract of the said microorganisms as an active ingredient, and its manufacturing method.

  The hair styling agent comprising a culture solution of microorganisms belonging to the genus Bacillus and / or an extract of the microorganisms as an active ingredient according to the present invention is well-familiar with hair and imparts gloss to the hair when applied to the hair. Excellent in feeling of use, long-lasting hair styling, and safe from virtually no ingredients that cause scalp irritation, hair loss and allergies without interfering with hair growth and hair growth It is a highly functional hair conditioner. Further, the hair styling agent of the present invention has an advantage that when applied to fine hair, soft hair, or thin hair, it imparts moderate hardness to the hair and gives the hair a sense of volume.

  The hair styling agent referred to in the present invention means all hair styling agents and styling agents used for hair styling purposes (hereinafter collectively referred to as “hair styling agents”). Examples of the microorganism belonging to the genus Bacillus according to the present invention include Bacillus subtilis and Bacillus natto, which are non-toxic microorganisms widely distributed in nature. Natto is a microorganism that is safe for human consumption. Microorganisms belonging to the genus Bacillus are also known as microorganisms that produce various useful enzymes in the field of starch industry. More specifically, the microorganism belonging to the genus Bacillus referred to in the present invention means all microorganisms belonging to the genus Bacillus, as long as the object of the present invention is not impaired, such as Bacillus globisporus, Bacillus macerans, Bacillus megaterium, Examples include microorganisms belonging to the genus Bacillus such as Bacillus circulans, Hacillus stearothermophilus, Bacillus coagulans, Bacillus natto, Bacillus subtilis. More specifically, for example, Bacillus species V230 (FERM BP-5054) Bacillus globisporus C9 (FERM BP-7143), Bacillus globisporus C9 (FERM BP-7143), Bacillus globisporus C11 (FERM BP-7144) Bacillus globisporus N75 (FERM BP-7591), Bacillus circulans P7 (FERM BP-10091), Bacillus circulans AM7 (FERM BP-10111), Bacillus stearothermophilus Tc-60 (FERM P- 2222), Bacillus stearothermophilus Tc-91 (FERM P-2225), and the microorganisms chosen from those variants can be illustrated.

  The medium used for culturing Bacillus microorganisms used in the present invention may be any nutrient medium capable of growing the microorganisms, and may be either a synthetic or natural medium. Any carbon source may be used as long as the microorganism can be used for growth. For example, plant-derived starch and hydrolyzate thereof, phytoglycogen, glycogen derived from animals and microorganisms, pullulan, erucinan, glucan, and other natural polysaccharides. Furthermore, partial decomposition products thereof, saccharides such as glucose, fructose, maltose, lactose, sucrose, mannitol, sorbitol, molasses, and organic acids such as citric acid and succinic acid can also be used. As the nitrogen source, for example, inorganic nitrogen compounds such as ammonium salts and nitrates, and one or more organic nitrogen-containing materials such as urea, corn steep liquor, casein, peptone, yeast extract and meat extract are used. It is done. Examples of the inorganic component include one or more of calcium salt, magnesium salt, potassium salt, sodium salt, phosphate, manganese salt, zinc salt, iron salt, copper salt, molybdenum salt, cobalt salt and the like. Used as appropriate. Furthermore, other components such as amino acids and vitamins are also used as necessary. The concentrations of the carbon source, nitrogen source, inorganic component, and other components in the medium are appropriately selected depending on their types. The medium used in the present invention is preferably a medium containing only the minimum necessary components when the microorganism grows.

  Cultivation of microorganisms belonging to the genus Bacillus used in the present invention is usually carried out aerobically under conditions selected from a temperature of 4 to 50 ° C., preferably 20 to 40 ° C., pH 4 to 10, preferably pH 5 to 9. Is called. The culture time may be longer than the time when the microorganism starts to grow, and is usually 6 hours or longer, preferably 12 to 150 hours. Further, the dissolved oxygen concentration of the culture solution is not particularly limited, but usually 0.5 to 20 ppm is preferable. The dissolved oxygen concentration is adjusted by means such as stirring, controlling the amount of ventilation, supplementing oxygen with ventilation, and increasing the pressure in the fermenter. The culture method may be either batch culture or continuous culture.

  The microorganism culture solution thus obtained is used as it is or after disrupting the cells by known appropriate means such as ultrasonic disruption or osmotic pressure destruction, and then subjected to membrane filtration such as centrifugation, precoat filter, flat membrane, hollow fiber membrane, etc. The insoluble matter such as the microbial cells or the crushed microbial cells is removed by a known appropriate means such as the separation means by, and the resulting supernatant is collected. The supernatant is concentrated as it is or concentrated by an appropriate concentration method such as vacuum concentration, flat membrane, hollow membrane or the like to obtain a concentrated solution. Since this concentrated liquid usually has a coloring and / or unpleasant odor, it is colorless when treated with a decoloring agent / deodorizing agent such as clay or activated carbon, or when deodorizing by steam distillation. It is possible to make an odorless high-quality concentrate. These concentrated solutions are usually sterilized or sterilized by known appropriate means such as heat sterilization, autoclaving, radiation sterilization, membrane filtration, and the like, as they are, and the culture solution of microorganisms belonging to the genus Bacillus of the present invention and 1 or a cosmetically acceptable gelling agent, thickener, softener, colorant, fragrance, stabilizer, preservative, foaming agent, etc. After blending appropriate amounts of seeds or two or more components, they are dried under normal pressure or reduced pressure by a known method to obtain liquid, gel, paste, mousse, block, granule, powder, etc. Used as a solid. At this time, before the final product is obtained, it is optional to perform the above-described sterilization or sterilization treatment.

  Furthermore, after the microorganisms in the culture solution obtained by the batch culture or continuous culture are intact or crushed, makeup tolerance such as water, alcohol such as methanol, ethanol, propanol, isopropyl alcohol or an aqueous alcohol solution of appropriate concentration is used. When alcohol is contained in the extract obtained by extraction with a medium, it is removed by an appropriate means such as a known dialysis method, a vacuum distillation method, a membrane separation method, and further, if necessary, the appropriate concentration method. To obtain an extract of a microorganism belonging to the genus Bacillus as an active ingredient in the hairdressing agent of the present invention. The hair styling agent of the present invention is characterized in that it does not substantially contain components such as alcohol, emulsifier, gelling agent and foaming agent that cause dryness, irritation, inflammation and allergy of the scalp. However, when the amounts of these components are so small that they do not depart from the object of the present invention, these hairdressing agents are also included in the present invention. In the present invention, “substantially free” means less than 1% (v / v), preferably less than 0.5% (v / v), more preferably 0.1% (v / v). ), More preferably less than 0.01%.

  The hair styling agent of the present invention is usually prepared by using a culture solution of microorganisms belonging to the genus Bacillus and / or an extract of the microorganisms as it is or with water, usually in a mass ratio ([culture solution of microorganisms belonging to the genus Bacillus and / or Microbial extract]: [water]), diluted in a ratio ranging from 50: 1 to 1:50, preferably 10: 1 to 1:10, more preferably 1: 3 to 1:10. To prepare. The water used in the present invention means makeup-acceptable water in general, specifically, purified water such as distilled water, deionized water and ultrapure water, and hair such as natural water (including mineral water). Means all water applicable to the scalp and skin. In addition, it is optional to add a proper amount of seawater (water solution derived from seawater containing deep seawater, seawater-derived bitter juice, minerals, etc.) to these waters. When these waters are used, the microorganisms belonging to the genus Bacillus are usually sterilized by applying known sterilization methods such as membrane filtration, heat sterilization, high pressure sterilization, ultraviolet sterilization, and radiation sterilization. The culture solution and / or the microorganism extract is diluted at a predetermined blending ratio, and the pH is neutral or weakly acidic, preferably pH 7 to 5, more preferably pH 6.9 to 5.1, More preferably, the hair styling agent of the present invention is adjusted to pH 6.0 to 5.2, more preferably pH 5.8 to 5.3. In addition, when deviating from the said pH, there exists a possibility that the expected effect which the hairdressing agent of this invention show | plays may reduce or the irritation | stimulation to a scalp may increase. In addition, when diluting a culture solution of microorganisms belonging to the genus Bacillus and / or an extract of the microorganisms with water, the hair styling agent of the present invention may be obtained by sterilization or sterilization by a known sterilization method after dilution. desirable.

  Moreover, the hairdressing agent of this invention can also be provided with the form of the portion type which divided | segmented the quantity suitable for one-time use into the container.

  In use, when the hair styling agent of the present invention is in a liquid, gel, paste or mousse form, an appropriate amount thereof is directly applied to the hair. Further, when the hair styling agent of the present invention is a solid product such as a block, granule, powder, etc., a cosmetic allowance mainly composed of an appropriate amount of water or water so that the blending ratio is obtained in use. Dilute with sex medium.

  Furthermore, in the hairdressing agent of the present invention, the active ingredient of the hairdressing agent of the present invention is stabilized as long as it does not impair the hairdressing action exerted by the culture solution of microorganisms belonging to the genus Bacillus and / or the extract of the microorganisms. In addition, for the purpose of imparting hair-restoration, hair-growth, dandruff and itching prevention, herbs extract, rosemary extract, plum extract, persimmon extract, pine extract and other extracts, glucose, maltose, mulch Sugars such as tall, lactosucrose, α, α-trehalose, α, β-trehalose, β, β-trehalose, α-glycosyl α, α-trehalose, cyclic oligosaccharides and cyclic oligosaccharide derivatives, rutin, α- Glycosyl rutin, hesperidin, α-glycosyl hesperidin, naringin, α-glycosyl naringin, quercetin, α-glycosyl ketone Contains one or more other ingredients selected from vitamins such as lucetin, L-ascorbic acid, α-glycosyl-L-ascorbic acid, perfume oils such as jojoba oil, minerals, takanal and procton olamine It is also optional to do. Among the above components, α, α-trehalose, α-glycosyl α, α-trehalose, and α-glycosyl-L-ascorbic acid are a culture solution of a microorganism belonging to the genus Bacillus used in the present invention and / or extraction of the microorganism. It is preferably used for the purpose of enhancing the storage stability of the product. Rutin, α-glycosyl rutin, hesperidin, α-glycosyl hesperidin, naringin, and α-glycosyl naringin have a function as a colorant, and can be suitably used in the present invention. The other component is usually in the range of 0.001 to 99% by mass, preferably 0.001 to 80% by mass, more preferably 0.001 to 50% by mass, based on the hairdressing agent of the present invention. Can be blended.

  Furthermore, in the hairdressing agent of the present invention, as long as the object of the present invention is not impaired, the fragrance, preservative, preservative, stabilizer, excipient, diluent, gelling agent blended in the conventional hairdressing agent. It does not prevent the blending of one or two or more components such as a hair color agent to the minimum necessary. Further, the makeup-acceptable medium used in the hair styling agent of the present invention means all media that are allowed to be blended in cosmetics. Examples of the hair conditioner of the present invention include liquids, gels, pastes, mousses, or solids such as blocks, granules, and powders. When the hairdressing agent of the present invention is liquid or mousse, it can be used in the form of a spray spray.

  Hereinafter, the present invention will be described in more detail based on experiments and examples.

<Experiment>
The culture solution of the microorganism belonging to the genus Bacillus obtained in Example 1 described later is diluted with 3 times, 5 times, and 10 times aseptic purified water, respectively, to obtain test solutions (“test solution 1”, “ Test solution 2 ”and“ Test solution 3 ”) were prepared, and a panel test was conducted using 20 healthy adults (12 men and 8 women) aged 20 to 60 years. As a control, a commercially available alcohol-containing hair styling agent (containing 3% or more of alcohol) (hereinafter referred to as “control solution”) was used. This panel test was conducted as follows. That is, 5 subjects (3 men, 2 women) were divided into 4 groups, and the hair of each group of subjects was washed by a barber using a commercially available shampoo, and the hair was air-dried with a dryer. Hair styling was performed using either No. 3 or the control solution, and “easy to do hair styling” was evaluated, and “lasting hair styling” was evaluated 6 hours after styling. The “scaling to the scalp” was determined based on the result of a barber observing the scalp of each subject and conducting a questionnaire survey on each subject. Regarding the “ease of hairdressing” and “persistence of hairdressing”, the barber is “good” (1 point), “not changed” (0 point), and “inferior” (−1) compared to the control (−1). Regarding “scalp irritation”, the number of respondents who answered that there was irritation to the scalp was shown in 5 subjects in each group. The results are shown in Table 1.

  As is clear from the results in Table 1, the culture solution of microorganisms belonging to the genus Bacillus used as a test solution is an excellent hair styling agent in terms of ease of hair styling and long-lasting hair styling, and irritation to the scalp. It turned out to be a safe hair styling agent. In addition, the ease of hair styling and the sustainability of hair styling performed by the culture solution of the microorganisms were concentration-dependent. Moreover, although specific data are not shown, it was pointed out that the subject who applied the culture solution of the microorganism effectively suppressed hair drying and had a moisturizing effect. Furthermore, although data is not specifically shown, when a patch test was conducted on 20 subjects under the observation of a doctor using the culture medium of the microorganism used in this experiment as it was, allergic reaction to the skin was I was not able to admit. These results indicate that the culture solution of microorganisms belonging to the genus Bacillus is useful and safe as an active ingredient of hairdressing agents.

<Hairdressing agent>
A liquid medium consisting of dextrin 3.0 w / v%, peptone 0.7 w / v%, dipotassium phosphate 0.2 w / v%, magnesium sulfate heptahydrate 0.05 w / v% and water, 100 ml triangular 30 ml in a flask, sterilized in an autoclave at 121 ° C. for 20 minutes, cooled, inoculated with Bacillus globisporus N75 (FERM BP-7591) as a microorganism belonging to the genus Bacillus, and cultured with shaking at 27 ° C. and 100 rpm for 48 hours. Thus, a seed culture solution was obtained. Separately, about 200 ml of medium having the same composition as in the case of seed culture was placed in three 500 ml Erlenmeyer flasks, heat sterilized, cooled to a temperature of 27 ° C., and then seed culture solution 1% (v / v ) And aerated and stirred for 72 hours while maintaining the temperature at 27 ° C. and pH 6.0 to 8.0. After culturing, the culture solutions were combined, centrifuged (20,000 rpm × 60 min), the cells and solids were removed, and the supernatant was collected. The supernatant was dialyzed against water at 4 ° C. for 10 hours, filtered through a membrane, adjusted to pH 5.5, sterilized, and filled into a 100 ml glass container.

  This product can be diluted as it is or with sterilized purified water or commercially available natural water, usually 3 to 10 times, and an appropriate amount can be applied to the hair to make hair styling. When this product is used for hairdressing, excellent hairstyling action is maintained for a relatively long time. In addition, this product can effectively suppress the drying of the hair and can keep the hair fresh and moisturized for a relatively long time.

<Hairdressing agent>
Except for using Bacillus globisporus C9 (FERM BP-7143) as a microorganism belonging to the genus Bacillus, Takanal and 200 ml of a microorganism culture solution (supernatant) obtained after centrifugation were obtained in the same manner as in Example 1. After adding and dissolving piroctone olamine to 0.02% (v / v) and 0.01% (v / v), respectively, an equivalent amount (volume ratio) of commercially available natural water was added. Mixed, membrane filtered, pH adjusted to 5.7, sterilized and filled into a 5 ml plastic container for a single use (4 ml).

  This product is a potion-type product, which can be diluted 3 to 10 times as it is or with sterilized purified water or commercially available natural water, and an appropriate amount thereof can be applied to the hair for hair styling. When this product is used for hairdressing, excellent hairstyling action is maintained for a relatively long time. In addition, this product can effectively suppress the drying of the hair and can keep the hair fresh and moisturized for a relatively long time. Furthermore, this product is a high-value-added hair styling agent that exhibits hair-growth and hair-restoring effects with Takanal, and also has anti-dandruff and itching-preventing effects with piroctone olamine.

<Hairdressing agent>
To 500 ml of the culture solution (supernatant) of the microorganism obtained in Example 1 after centrifugation, trehalose is 0.02% (v / v) and α-glycosylrutin is 0.01% (v / v). Aseptically added to and dissolved. To 20 parts by mass of the resulting solution, 3 parts by mass of beeswax, 18 parts by mass of liquid paraffin, 2 parts by mass of deep sea water and 4 parts by mass of microcrystalline cellulose are added and mixed, sterilized by heating, and aseptically adjusted to pH 5. .3 and filled into a 200 ml plastic container.

  When this product is used for hairdressing, excellent hairstyling action is maintained for a relatively long time. In addition, this product, combined with the moisturizing action of trehalose, can effectively suppress the drying of the hair and can keep the hair fresh and moisturized for a relatively long time. This product is a high value-added hair styling agent that also promotes blood circulation of the scalp by α-glycosyl rutin.

<Hairdressing agent>
A liquid medium consisting of maltose 3.0 w / v%, peptone 0.7 w / v%, dipotassium phosphate 0.2 w / v%, magnesium sulfate heptahydrate 0.05 w / v% and water, 100 ml triangular 30 ml was put into a flask, sterilized in an autoclave at 121 ° C. for 20 minutes, cooled, inoculated with Bacillus globisporus C11 (FERM BP-7144) as a microorganism belonging to the genus Bacillus, and rotated and cultured at 27 ° C. and 100 rpm for 48 hours. Thus, a seed culture solution was obtained. Separately, about 200 ml of medium having the same composition as in the case of seed culture was placed in three 500 ml Erlenmeyer flasks, heat sterilized, cooled to a temperature of 27 ° C., and then seed culture solution 1% (v / v ) And aerated and stirred for 72 hours while maintaining the temperature at 27 ° C. and pH 6.0 to 8.0. After culturing, the culture fluids are combined, collected by centrifugation (3,000 rpm × 30 min), the cells are sonicated, and centrifuged again (20,000 rpm × 60 min). -The crushed cells and solids were removed, and the supernatant was collected. The supernatant was dialyzed against water at 4 ° C. for 10 hours, and α-glycosyl hesperidin and α-glucosyl α, α-trehalose were added to a concentration of 10% (v / v), and the pH was 5.8. , Filtered, sterilized, and filled into a 100 ml glass container.

  This product can be diluted as it is or with sterilized purified water or commercially available natural water, usually 3 to 10 times, and an appropriate amount can be applied to the hair to make hair styling. When this product is used for hairdressing, excellent hairstyling action is maintained for a relatively long time. In addition, this product can effectively suppress the drying of the hair and can keep the hair fresh and moisturized for a relatively long time.

<Hairdressing agent>
A liquid medium consisting of dextrin 3.0 w / v%, peptone 0.7 w / v%, dipotassium phosphate 0.2 w / v%, magnesium sulfate heptahydrate 0.05 w / v% and water, 100 ml triangular 30 ml in a flask, sterilized in an autoclave at 121 ° C. for 20 minutes, cooled, inoculated with Bacillus natto as a microorganism belonging to the genus Bacillus, and cultured with shaking at 27 ° C. and 100 rpm for 48 hours. Obtained. Separately, about 200 ml of medium having the same composition as in the case of seed culture was placed in three 500 ml Erlenmeyer flasks, heat sterilized, cooled to a temperature of 27 ° C., and then seed culture solution 1% (v / v ) And aerated and stirred for 72 hours while maintaining the temperature at 27 ° C. and pH 6.0 to 8.0. After culturing, the culture fluids are combined, collected by centrifugation (3,000 rpm × 30 min), the cells are sonicated, and centrifuged again (20,000 rpm × 60 min). -The crushed cells and solids were removed, and the supernatant was collected. The supernatant was dialyzed against water at 4 ° C. for 10 hours, trehalose was added to a concentration of 5% (v / v), the pH was adjusted to 5.6, membrane filtered, sterilized, A 100 ml glass container was filled.

  This product can be diluted as it is or with sterilized purified water or commercially available natural water, usually 3 to 10 times, and an appropriate amount can be applied to the hair to make hair styling. When this product is used for styling, excellent hair styling action lasts for one year. In addition, this product can effectively suppress the drying of the hair and can keep the hair fresh and moisturized for a relatively long time.

  As described above, the hair styling agent of the present invention has an excellent function as a hair styling agent, effectively suppresses drying of the hair, and is a safe hair styling agent that does not substantially cause scalp irritation, hair loss, or allergy. Is to provide. The present invention also provides a method for producing such a hairdressing agent.

  The present invention is an invention that exhibits such remarkable effects, and it is a very significant invention that contributes to this field.

Claims (9)

  A hair styling comprising a culture solution of a microorganism belonging to the genus Bacillus and / or an extract of the microorganism as an active ingredient.   A culture solution of a microorganism belonging to the genus Bacillus is cultured in a liquid nutrient medium, and after the cells are intact or crushed, insolubles are removed, and the resulting supernatant is collected and sterilized or sterilized. The hair styling agent according to claim 1, wherein the hair styling agent is obtained as a solution.   The hair styling agent according to claim 1, wherein the extract of a microorganism belonging to the genus Bacillus is extracted with a makeup-acceptable medium selected from water, alcohol, and an aqueous alcohol solution after the microorganism is intact or crushed.   4. The hair styling agent according to claim 1, wherein the alcohol content is less than 1%.   Takanal, piroctone olamine, jojoba oil, herbal extract, maltose, maltitol, lactosucrose, α, α-trehalose, α-glycosyl α, α-trehalose, cyclic oligosaccharide, rutin, α-glycosyl rutin, hesperidin, α The glycosyl hesperidin, naringin, α-glycosyl naringin, quercetin, α-glycosyl quercetin, α-glycosyl-L-ascorbic acid, and one or more components selected from minerals are further included. The hair styling agent in any one.   The hair styling agent according to any one of claims 1 to 5, which has a pH of 6.0 to 5.2.   The hair styling agent according to any one of claims 1 to 6, wherein the hair styling agent is in the form of a liquid, a paste, a gel, a mousse, a block, a granule or a powder.   A step of culturing a microorganism belonging to the genus Bacillus in a liquid nutrient medium, a step of removing the bacterial cells or crushed cells after the cells are crushed as they are, a step of collecting the supernatant thereof, and further if necessary A method for producing a hair styling product comprising a step of diluting with a makeup-acceptable medium.   The method for producing a hairdressing agent according to claim 8, further comprising a step of decoloring and / or deodorizing the supernatant after the step of collecting the supernatant.