JP2006298814A - Skin lotion for acne - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To utilize the extract of mango (Mangifera indica L.) effectively, of which effectiveness only as a health food is found until now. <P>SOLUTION: This mango extract has a lipase inhibitory effect and it is found out to improve acne, etc. Further by blending 1 kind or ≥2 kinds of any of specific medicines such as a humectant, a cell-activator, an anti-inflammatory agent, an antioxidant, a beautifully whitening agent, a hyaluronidase inhibitor, an anti-plasmin agent, an active oxygen inhibitor, a collagenase activity inhibitor, an antihistamine agent and an elastase inhibitor with the extract, a more effective skin lotion is obtained. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

The product of the present invention has an extract extracted from a plant of a specific genus that preferentially inhibits the production of lipases from skin resident bacteria, thereby suppressing acne formation, and a moisturizer, anti-inflammatory agent, antioxidant agent Further, the present invention relates to an external preparation for skin which can further improve and prevent rough skin by combining and combining the specified agents of antiplasmin agent, active oxygen inhibitor and antihistamine.

Mango (Mangifera indica L.) is a plant of the family Uranaceae (Anacardiaceae), Mango genus (Mangifera), Mango (Mangifera indica L.). Is said to be the most delicious fruit produced in Japan. The mango extract is known for its use in cosmetics, bath preparations, and detergent compositions (see Patent Documents 1 and 2). In addition, the mango seed extract includes a composition for lipstick (see Patent Document 3), a hair / scalp cosmetic having an effect of repairing damaged hair (see Patent Document 4), and an antioxidant composition (see Patent Document 5). The use for bacteriostatic and antibacterial agents (see Patent Document 6) is known, but other uses have not been known so far.

Acne is an inflammatory disease of the follicular gland system that occurs in areas with many sebaceous glands such as the face and back. P. acnes, which is deeply involved in acne pathology, is a resident skin resident in healthy human skin and produces bacterial lipase. The produced lipase breaks down triglycerides in sebum to produce free fatty acids, which not only march and form comed but also cause acne inflammation. Inflammation caused by acne can irritate the skin and even leave it as acne scars, preventing it from maintaining healthy skin.
Japanese Patent Laid-Open No. 2001-31552 JP 2001-39823 A Japanese Patent Laid-Open No. 7-223924 JP-A-9-208435 Japanese Patent Laid-Open No. 9-216836 Japanese Patent Laid-Open No. 10-324610

The object of the present invention has been variously studied for effective use with respect to an extract of highly effective mango (Mangifera indica L.).

It was found that an extract of mango (Mangifera indica L.) inhibits lipase activity and was effective as a skin external preparation for acne.
Furthermore, a more effective skin external preparation was found by adding specific drugs (humectants, anti-inflammatory agents, antioxidants, antiplasmin agents, active oxygen inhibitors, antihistamines, etc.) to mango.

The form of the mango extract is not limited in any way. Preferably, the mango fruit is shade-dried, ground and then immersed in an extraction solvent (for example, alcohol such as ethanol, water or a mixture thereof) for 3 days or 100 hours for 1 hour. Use liquid that has been heated, cooled, and filtered.

Test: Lipase Inhibition Test Extract of mango (Mangifera indica L.) by lipase inhibition test in which the substrate Pentanoic acid 4-nitrophenyl eater is hydrolyzed by enzyme lipase and the absorption of 4-nitrophenyl ester produced is measured. Lipase activity inhibitory action was examined. Add 500 μl of the same buffer 3 ml, 2 mg / ml lipase [SIGMA] enzyme-50 mM Tris buffer (pH 7.7) to 500 μl of the test product prepared with 0.04% olefin sulfonic acid-50 mM Tris buffer (pH 7.7). After stirring, the mixture was incubated at 40 ° C. for 5 minutes. Further, 1 ml of 1 mM PNP-valerate was added as a substrate, stirred and incubated at 40 ° C. for 15 minutes, and then the absorbance (A) was measured at Abs. 425 nm. Similarly, Absorbance (B) when buffer is used as an alternative to enzyme solution, Absorbance (C) when buffer is used as alternative to test product, Buffer solution is used instead of enzyme solution, and Test product is used instead The absorbance (D) when a buffer solution was used was measured, and the lipase activity inhibition rate (%) was calculated from the following formula.
<Formula> Lipase activity inhibition rate (%) = [1- (AB) / (CD)] × 100
○ Test items are shown below.
Mango E: Mango (dried fruit) (20 g) in ethanol (100 ml) extract (freeze dried)
Mango EW: Mango (fruit dried product) (20 g) 50% ethanol-water (100 ml) extract (lyophilized product)

  As a result of the lipase activity inhibition rate in the above test, mango E was 71.2% and mango EW was 50.3%.

Mango extract (Mangifera indica L.) showed high lipase inhibitory effect. Mango extract (Mangifera indica L.) extract is desirable to add at this concentration because it can sufficiently exert an inhibitory effect and the like when added at 0.0001 to 10% by weight.

In addition, when a specific drug (humectant, cell activator, anti-inflammatory agent, antioxidant, whitening agent, antiplasmin agent, active oxygen inhibitor, antihistamine, etc.) is added to the mango (Mangifera indica L.) extract It was found to improve or prevent various skin problems such as acne, inflammation and rough skin.

Furthermore, it has also been found that the effect can be further obtained by blending one or more of polyhydric alcohols containing two or more hydroxyl groups, lower alcohols, surfactants, and low molecular betaines.

  The polyhydric alcohol that can be used is not particularly limited, and examples thereof include glycerin, 1,3 butylene glycol, propylene glycol, isoprene glycol, sorbitol, trehalose, maltose and the like. These are blended in an amount of 0.1 to 80% by weight, preferably 1 to 30% by weight. Needless to say, the blending amount varies depending on the type of polyhydric alcohol, and a plurality of polyhydric alcohols can be used.

Absorbability improves by mix | blending a lower alcohol. Although there are various kinds of lower alcohols, ethanol is usually used. The blending amount is not particularly limited but is preferably 2 to 70% by weight.
It is also effective to combine a surfactant with a polyhydric alcohol combination containing an extract of mango (Indica L.), and it is also effective to combine the lower alcohol.
There is no particular limitation on the surfactant, and the type and amount may be selected depending on the application and dosage form.
Surfactants are used for emulsification and solubilization of oils, etc., and are anionic (alkyl carboxylates, alkyl sulfonates, alkyl sulfate esters, alkyl phosphate esters), nonionic (ether type) Nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant) and amphoteric (carboxylic acid type amphoteric surfactant) Agents (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant) and other surfactants (natural surfactants, protein hydrolysates) Derivatives, polymer surfactants, surfactants containing titanium and silicon, and fluorocarbon surfactants) can be used as appropriate.

Low molecular weight betaine is also one of the raw materials to be selected, and by blending this, the effect is further improved and the feeling of application is improved, and the effectiveness as an external preparation for skin is increased.
Low molecular weight betaines that can be utilized may include those that form zwitterions with internal salts such as quaternary ammonium bases, sulfonium bases, phosphonium bases and the like having a molecular weight of 200 or less. Examples include trimethylglycine, N, N, N-trimethylalanine, N, N, N-trimethylglutamic acid, triethylglycine, and the like, with trimethylglycine being preferred.
Those having a molecular weight of 200 or more have a surface-active ability, which is different from the effect of low molecular betaine. As described above, the use of a surfactant enhances the effect of the present invention, but the effect of low molecular betaine is Is different.

  The external preparation for skin of the present invention can be prepared by blending with various forms of base materials known as normal external preparations for skin according to conventional methods.

It does not specifically limit as a form of an external preparation, For example, arbitrary dosage forms, such as a milky lotion, cream, aqueous solution, a pack, can be selected.

In the external preparation for skin of the present invention, in addition to the above-mentioned essential components, components that are blended in normal external preparations, such as oils, powders, purified water, polymer compounds, gelling agents, UV absorbers, UV scattering Agents, antioxidants, pigments, preservatives, fragrances, and cosmetic ingredients can be appropriately selected and used within a range not impairing the effects of the present invention.

EXAMPLES Next, although an Example and a comparative example are given and this invention is demonstrated still in detail, this invention is not restrict | limited at all to these. Moreover, the extraction method about the extract of the used chemical | medical agent is not limited at all.
Tables 1 and 2 show the formulations of Examples and Comparative Examples. The preparation method was performed by a conventional method. Table 1 shows the lotion and Table 2 shows the formulation of the cosmetic liquid, and the amount is shown in parts by weight.

Details of the raw materials used in the above comparative examples and examples are described below.
(Note 1) As the mango extract, 200 ml of ethanol was added to 10 g of mango (fruit dried product) and stirred for 5 days, and then the filtered extract was used.
(Note 2) As the mango extract, 100 ml of ethanol and 100 ml of purified water were added to 10 g of mango (fruit dried product) and stirred for 5 days, and then the filtered extract was used.
(Note 3) The hydrolyzed conchiolin solution used was one having a molecular weight of 1000 or less, by hydrolyzing conchiolin.
(Note 4) [Tochimoto Tenkaido Co., Ltd.] was used as the Rhizono extract.
(Note 5) As the honoki extract, Falco Rex honoki E [manufactured by Ichimaru Falcos Co., Ltd.] was used.
(Note 6) As the guava extract, 300 ml of ethanol was added to 10 g of guava leaves and allowed to stand for 5 days, and then the filtered extract was used.
(Note 7) Nishi-Hayanagi extract [Nippon Seika Co., Ltd.] was used as the extract.
(Note 8) Marine purge [manufactured by Ichimaru Falcos Co., Ltd.] was used as the seaweed extract.

The acne improvement test was implemented about Examples 1-8 of Tables 1-2, and Comparative Examples 1-2. The test method consisted of 20 females aged 25 to 55 years old, and a suitable amount of a topical preparation was applied to the face after washing the face twice a day in the morning and night for 12 weeks. The results are shown in Table 3.

Claims (3)

A skin external preparation for acne, which is characterized by containing an extract of mango (Mangifera indica L.). Lipase inhibitor characterized by containing an extract of mango (Mangifera indica L.) An external preparation for skin containing one or more of mango extract (Mangifera indica L.) and moisturizer, anti-inflammatory agent, antioxidant agent, antiplasmin agent, active oxygen inhibitor, antihistamine agent