JP2006204310A - Method for cultivating plant worm - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To shorten a cultivation period by irradiation with a proper light in each step of cultivation, springing and growth to improve the productivity by increasing the yield. <P>SOLUTION: A medium is continuously or intermittently irradiated with yellow or red light having a peak in 540-720 nm wave length range to propagate and mature hypha when inoculating the hypha of the plant worm in the medium for artificially culturing the plant worm and culturing the hypha. After the culture of the hypha, the medium is continuously or intermittently irradiated with blue or green light by a light source having a peak in a wave length range of 300-550 nm at the springing step and the growth step. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  TECHNICAL FIELD The present invention relates to a method for cultivating cordyceps that improves productivity by using light wavelengths that are effective along the course of cultivation.

  In general, it is already known that light is important as an element for determining the quality of mushrooms in the cultivation of mushrooms. In conventional techniques, light is used in each step of culturing, generating (sprouting) and growing. Was used.

  In addition, it is known that light that is effective for mushrooms is light in the blue range, but in actual cultivation, considering the cost in terms of workability and productivity, it is necessary to use an inexpensive white fluorescent lamp. Since it was the most realistic, white fluorescent lamps were generally used for mushroom cultivation.

  On the other hand, in the cultivation of Cordyceps sinensis, the effect of white fluorescent lamps is known, but there is no previous test report on how other colors of light affect the cultivation. For the Cordyceps sinensis, which has become possible to produce, it has been expected to develop a cultivation method in a more optimal environment.

  However, in the culture of Cordyceps sinensis, white fluorescent lamps were used as the lighting in the culture room. Cordyceps emerged before the hyphae spread, resulting in irregularities in germination and growth, resulting in problems such as reduced yield.

  Furthermore, in the generation and growth processes, white fluorescent lamps with a wide wavelength range contain both the wavelength of the promotion effect and the wavelength of the suppression effect that are specific to Cordyceps, so that the generation efficiency is higher than that of monochromatic light. There was a problem of causing troubles that deteriorated productivity.

  In view of such a conventional problem, the present invention is intended to improve productivity by shortening the culture period and increasing the yield by performing appropriate light irradiation in each step of culture, generation, and growth. It was made as a purpose.

  The feature of the method for cultivating Cordyceps medicinal plant according to claim 1 for solving the conventional problems as described above and achieving the intended purpose is to inoculate Cordyceps medicinal plant fungus into a culture medium for Cordyceps medicinal plant artificial cultivation and cultivate mycelia. In this case, the medium is irradiated with light from a light source composed of a colored fluorescent lamp having a peak in the wavelength range of 540 to 720 nm, and after the mycelial culture, a peak is observed in the wavelength range of 300 to 550 nm during the generation process. Light is emitted from a light source composed of a light emitting diode, and light is irradiated from a light source composed of a light emitting diode having a peak in a wavelength range of 300 to 550 nm in the growth step after the generation step.

  In this way, in the culturing process, by continuously or intermittently irradiating yellow or red light with a light source having a peak in the wavelength range of 540 to 720 nm, germination during the culturing or ripening process is suppressed and subsequent cultivation is performed. The process can be cultivated stably and in high yield.

  In addition, after the mycelial culture, during the generation process, the generation is accelerated by irradiating blue or green light continuously or intermittently with a light source having a peak in the wavelength range of 300 to 550 nm, which occurs compared to the conventional method. Efficiency is improved and productivity is improved.

In addition, in the growth process after the generation process, the quality of cordyceps in the growth is improved by continuously or intermittently irradiating blue or green light with a light source having a peak in the wavelength range of 300 to 550 nm, and the yield is increased. Can be made.
In addition, it is preferable to continuously or intermittently irradiate blue or green light with a light source having a peak in the wavelength range of 300 to 550 nm after the mycelial culture, during the generation process. The generation efficiency is improved compared to the above, and productivity is improved.

  Further, the light source used in the present invention can achieve a certain purpose even with a colored fluorescent tube, but it is more preferable to use a light emitting diode as a light source, and thereby even better results can be obtained.

  Furthermore, it is preferable to use a cultivation shelf in which light-emitting diodes are arranged so that the illuminance of light irradiated in the growing process is within 100 lux difference in each part at the maximum output, thereby moreover the entire shelf. Uniform mycelial culture, fruiting body development and growth will be achieved.

  Furthermore, the light-emitting diodes installed on the cultivation shelf are arranged by arranging a plurality of light-emitting diodes of monochromatic light such as blue, green, red, yellow, orange, etc., as appropriate, and the light-emitting diodes are arranged in any combination of single color or multiple colors. It is preferable to emit light at the same time. If light is emitted appropriately for each single color, fine adjustment of the quality finish becomes possible, and the quality can be further improved.

  According to the method for cultivating cordyceps according to the present invention, by appropriately irradiating light of a wavelength that works effectively in each step in the cultivation process of mushrooms or cordyceps, the productivity of cordyceps can be improved, In the germination process, the amount of generation is increased in the germination process, and the yield is increased in the growth process.

  Furthermore, if a light-emitting diode is used as a light source, it can be installed in a smaller size than a fluorescent lamp so that it can be uniformly irradiated to a cultivation bottle. Cultivation is possible, the light source is almost short wavelength, so it is possible to irradiate light with the required wavelength efficiently. Reduces energy loss and reduces energy loss compared to fluorescent lamps, which requires less than 10% of the electricity used, reduces running costs, and can be used semi-permanently, so there is no need to replace fluorescent tubes. The effect of not occurring is obtained.

  Next, the method for cultivating Cordyceps medicinal plants according to the present invention will be described in detail by taking an example of a bamboo shoot. In addition, this invention is not limited to this, It is effective similarly to other Isalia genus, Cordyceps genus, etc. In addition, it is possible to arbitrarily change the details of the method and the like without departing from the spirit of the present invention.

Cordyceps culture test (comparison of hyphal elongation)
During the mycelial culture of Cordyceps sinensis, the effect of light irradiation of each color on mycelial elongation was tested.

The potato dextrose was inoculated with Cordyceps fungus (Hanasanagitake fungus), and the elongation after 1 week was measured. The results are shown in Table 1. The light source used was a light emitting diode and a white fluorescent lamp.

  The control and Examples 1 to 4 were cultivated as follows. Table 2 summarizes the light irradiation and harvesting situation in each example.

Control Example A white fluorescent lamp was used during the culturing process and was lit only when the operator entered the room. During the generation process, a white fluorescent lamp was used as a light source, and it was always lit at 50 lx (unit looks). During the growth process, a white fluorescent lamp was used as a light source, and light was radiated intermittently at an illuminance of 500 to 2000 lx for 15 minutes and 45 minutes.

  The yield was 6.3 g / medium 50 g, and there was an occurrence before the hyphae spread.

Example 1
During the culture process, a yellow colored fluorescent lamp (having a peak at a wavelength of 570 nm) and a red colored fluorescent lamp (having a peak at a wavelength of 660 nm) were used, and the lamp was turned on only when an operator entered the room. During the generation process, a white fluorescent lamp was used as the light source, and it was always lit at 50 lx. During the growth process, a white fluorescent lamp was used as a light source, and light was radiated intermittently at an illuminance of 500 to 2000 lx for 15 minutes and 45 minutes.

  The yield was 7.2 g / medium 50 g in the yellow fluorescent lamp culture group and 7.5 / medium 50 g in the red fluorescent lamp culture group, and there was no occurrence before the hyphae spread.

Example 2
A yellow colored fluorescent lamp (having a peak at a wavelength of 570 nm) and a red colored fluorescent lamp (having a peak at a wavelength of 660 nm) and a red colored fluorescent lamp (having a peak at 660 nm) were used during the culturing process, and the lamp was always lit at an illuminance of 50 lx or less. During the generation process, a white fluorescent lamp was used as the light source, and it was always lit at 50 lx. During the growth process, a white fluorescent lamp was used as a light source, and light was radiated intermittently at an illuminance of 500 to 2000 lx for 15 minutes and 45 minutes.

  The yield was 7.8 g / medium 50 g in the yellow fluorescent light culture group and 7.7 / medium 50 g in the red fluorescent light culture group. There was no occurrence before the hypha spread, and the culture medium was shorter for 2 days than the control example. .

Example 3
During the culture process, a yellow colored fluorescent lamp (having a peak at a wavelength of 570 nm) was used, and it was always lit at an illuminance of 50 lx or less. During the generation process, a light emitting diode was used as a light source, and blue (having a peak at a wavelength of 460 nm) and green (having a peak at a wavelength of 520 nm) were always lit with an illuminance of 10 lx or less. During the growth process, a white fluorescent lamp was used as a light source, and light was radiated intermittently at an illuminance of 500 to 2000 lx for 15 minutes and 45 minutes.

  The yield was 8.2 g / medium 50 g in the blue light-emitting diode generation area and 8.5 / medium 50 g in the green light-emitting diode generation area, and there was no occurrence before the hyphae spread.

Example 4
During the culture process, a yellow colored fluorescent lamp (having a peak at a wavelength of 570 nm) was used, and it was always lit at an illuminance of 50 lx or less. During the generation process, a light-emitting diode was used as a light source, and green light (having a peak at a wavelength of 520 nm) was always lit with an illuminance of 10 lx or less. During the growth process, a light emitting diode was used as a light source, and blue (having a peak at a wavelength of 460 nm) and green (having a peak at a wavelength of 520 nm) were constantly lit at an illuminance of 10 lx or less.

The yield was 8.4 g / medium 50 g in the blue light-emitting diode generation area and 8.5 / medium 50 g in the green light-emitting diode generation area, and there was no occurrence before the hyphae spread.

Use apparatus Next, the cultivation shelf used in each Example mentioned above is demonstrated about drawing.

  In the figure, reference numeral 1 denotes a cultivation shelf in which pipes are assembled as pillars and cross members. In this, cultivation bottle placement shelves 2, 2... Are provided in a multi-stage arrangement, and a plurality of containers (containers) 3 having a predetermined number of flat box-like culture media can be placed side by side for each special stage.

  A light source support board 5 is fixed downward on each shelf 2, and light emitting diodes 6, 6... That are light emitters are fixed to each board 5. The light emitters are arranged and fixed so that the difference is 100 lx.

  Light emitting diodes that use light emitting diodes can be installed in combination with a plurality of blue, green, red, yellow, orange single color light emitting diodes 6, 6 ... The amount of light can be adjusted, and irradiation light composed of a single color or a combination of a plurality of colors can be appropriately selected and used.

  This makes it possible to give an appropriate amount of irradiation to mushrooms during cultivation with light of an appropriate wavelength at an appropriate time.

It is a side view of an example of the cultivation shelf used for the present invention. It is the sectional view on the AA line in FIG. It is a bottom view of the light source support board which supported many light emitting diodes as a light source.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Cultivation shelf 2 Cultivation bottle mounting shelf 3 Medium containing container 5 Light source support board 6 Light emitting diode

Claims (2)

  When inoculating Cordyceps fungus on a medium for artificial cultivation of Cordyceps sinensis and culturing mycelium, the medium is irradiated with light from a colored fluorescent lamp having a peak in the wavelength range of 540 to 720 nm, Thereafter, light is emitted from a light source composed of a light emitting diode having a peak in the wavelength region of 300 to 550 nm during the generation step, and the light emitting diode having a peak in the wavelength region of 300 to 550 nm in the growth step after the generation step. A method for cultivating Cordyceps sinensis characterized by irradiating light with a light source comprising:   Light-emitting diodes use a cultivation shelf in which a large number of light-emitting diodes of monochromatic light such as blue, green, red, yellow, and orange are appropriately combined and arranged, and the light-emitting diodes emit light in any combination of single color or multiple colors The method for cultivating Cordyceps sinensis according to claim 1, wherein light irradiation in each step is performed.

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