JP2006199626A - Composition for ameliorating dermal function containing ceramide of acetic acid bacterium - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition for ameliorating the dermal function, having beautification effects such as humidity retention, prevention and amelioration of rough skin, and prevention and amelioration of wrinkles by being orally administered. <P>SOLUTION: The composition for ameliorating the dermal function contains a ceramide of acetic acid bacteria, the ceramide of the acetic acid bacteria, extracted from the acetic acid bacteria by using a polar solvent, or a cell-pulverized product of the acetic acid bacteria containing the ceramide of the acetic acid bacteria as an active ingredient. Water, glycerol, an alcohol, a ketone, glacial acetic acid or a mixed solvent thereof is used as the solvent. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a composition for improving skin function, which has cosmetic effects such as moisturizing, improvement of rough skin prevention, improvement of prevention of wrinkles, etc. by oral ingestion.

  Plant-derived ceramide is a component of human skin organs and is known to have cosmetic effects such as moisturizing, preventing rough skin, and preventing wrinkles by oral ingestion (for example, Non-Patent Document 1 and Non-Patent Document 1). Reference 2).

  Ceramide refers to lipids excluding fat, glycerolipid, and sterol lipid. Ceramide is extracted from plants such as wheat, rice and soybean as described above, and extracted from animals such as cow brain. And eukaryotic microorganisms such as yeast and those extracted from prokaryotic microorganisms such as Sphingomonas. However, ceramides derived from animals such as bovine brain tend to be avoided due to BSE problems, and microorganisms such as Sphingomonas are avoided from oral intake due to their lack of dietary experience.

  Therefore, ceramides extracted from plants such as wheat, rice, and soybean have been used when expecting beauty effects such as moisturizing, preventing rough skin, and preventing wrinkles by oral ingestion.

  However, when ceramide extracted from plants such as wheat, rice, and soybean is orally ingested, the effect is not sufficient, and a more effective one has been demanded.

The present invention relates to a skin function-improving composition characterized by comprising ceramide derived from acetic acid bacteria as an active ingredient, and in acetic acid ceramide, it has a very remarkable cosmetic effect over plant ceramide, It has not been known so far that the effect is achieved by oral ingestion and is novel.
"Fragrance Journal", Volume 23, p. 81-89, 1995 “Bioindustry”, Volume 19, p. 16-26, 2002

  The object of the present invention is to provide a composition for improving skin function, which is more effective than plant ceramide and has effects such as moisturizing, improvement of rough skin prevention, improvement of prevention of wrinkles, etc. when taken orally.

  The present inventors have focused on acetic acid bacteria that have been used since ancient times as brewing vinegar-producing bacteria and are recognized to be highly safe.

  And the present inventors have found that by ingesting acetic acid bacteria ceramide extracted from acetic acid bacteria used for the production of brewed vinegar, there are cosmetic effects such as moisturizing, improvement of rough skin prevention, improvement of wrinkle prevention, etc. The present invention has been completed.

That is, the present invention according to claim 1 relates to a skin function improving composition comprising acetic acid ceramide as an active ingredient.
Moreover, this invention of Claim 2 is related with the composition for skin function improvement characterized by containing the cell crushed material of the acetic acid bacteria containing an acetic acid ceramide as an active ingredient.
Furthermore, the present invention described in claim 3 relates to the composition for improving skin function according to claim 1, wherein acetic acid ceramide is extracted from acetic acid bacteria using a polar solvent.
Moreover, this invention of Claim 4 is food-drinks, It is related with the composition for skin function improvement of Claims 1-3 characterized by the above-mentioned.
And this invention of Claim 5 reviews the composition for skin function improvement of Claim 4 characterized by the food-drinks being vinegar.

  The skin function improving composition containing acetic acid ceramide of the present invention has cosmetic effects such as moisturizing, preventing rough skin, and preventing wrinkle by directly ingesting acetic acid ceramide. The same skin function improving effect can be obtained even if a cell destruction product of acetic acid bacteria containing acetic acid bacteria ceramide or a polar solvent extract of acetic acid bacteria is contained as an active ingredient.

  Hereinafter, the present invention will be described in detail.

  The acetic acid bacteria ceramide used in the present invention has a structure in which a sphingine precursor, a sphingosine precursor of sphingoid base moiety of human ceramide, and a fatty acid are amide-bonded, and can be obtained, for example, as follows.

  For example, acetic acid bacteria may be used as a raw material and extracted using a polar solvent, and this may be used as acetic acid bacteria ceramide, or the obtained extract may be further recrystallized with an organic solvent.

  As the organic solvent, all organic solvents such as alcohol, aliphatic carboxylic acid ester and ketone can be used alone or in combination of two or more. Examples of the alcohol include various lower alcohols such as methanol, ethanol, propanol, isopropanol, and butanol. Examples of the aliphatic carboxylic acid ester include branched or straight chain such as formic acid, acetic acid, propionic acid, butyric acid, valeric acid, and caproic acid. In addition to the saturated fatty acids, alcohol esters of various unsaturated fatty acids, for example, esters of the above alcohols are exemplified.

  Examples of the ketone include acetone, ethyl methyl ketone, methyl propyl ketone, isopropyl methyl ketone, butyl methyl ketone, and isobutyl ketone.

  As the polar solvent used for the extraction of acetic acid ceramide, all polar solvents such as water, glycerin, alcohol, ketone, glacial acetic acid can be used alone or in combination of two or more. As alcohol and ketone, those exemplified above can be used as appropriate.

  In order to extract acetic acid bacteria ceramide from acetic acid bacteria using a polar solvent, for example, acetic acid bacteria or the acetic acid bacteria are appropriately crushed with ultrasonic waves, and then acetic acid ceramide is extracted with a polar solvent. The extracted liquid obtained after extraction can be used as it is as acetic acid ceramide.

  The extraction may be performed by sufficiently bringing wet or dry cells into contact with a solvent, and conventional methods are appropriately applied. For example, in the case of dry cells, 0.5 to 30 liters of organic solvent (if necessary, 0.1 to 10 times, usually adding the same amount of water) to 1 kg of cells, usually Add about 5-15 liters, mix well, then perform separation treatment such as centrifugation or filtration to recover the upper layer, or use a Soxhlet extractor to produce 10 kg of dry cells with the same amount as above. After adding an organic solvent such as alcohol and heating to reflux, the resulting extract is dried under reduced pressure, and then dissolved by adding 0.5 to 15 liters, usually 2 to 8 liters of an organic solvent such as ketones. The filtrate (supernatant) after separating the precipitate may be recovered as an extract.

  In addition, after extraction using a solvent (for example, a mixture of chloroform, alcohol, and water), the lower layer is recovered as a crude lipid fraction, and caustic soda, caustic potash, and other alkaline solutions are added to weakly decompose the alkali to stabilize the alkali. Lipid is obtained, which may be acetic acid ceramide. Further, if desired, this may be treated with silica gel column chromatography or the like. For example, after washing with a mixed solvent of chloroform and acetic acid, the fraction eluted with the mixed solvent of chloroform and alcohol may be used as ceramide of acetic acid bacteria. Good.

  The extract thus obtained can be used as it is as acetic acid bacteria ceramide, but further, a processed product of the extract can also be used. Examples of the processed product include a concentrate of the extract, Pasted products, dried products and diluted products can be used, and purified products such as acetic acid ceramides can be separated and purified by liquid chromatography, countercurrent distribution, etc., and collected and used as acetic acid ceramides. it can. In the present invention, acetic acid ceramide includes not only purified products but also crude products and contained products, and these treated products are also included.

  In addition, since acetic acid bacteria ceramide is present in acetic acid bacteria, particularly in the cell membrane thereof, the acetic acid bacteria may be used as it is as acetic acid bacteria ceramide or may be contained without passing through the extraction step. Furthermore, taking into account absorption in the body, high-pressure crushers such as high-pressure homogenizers and French presses, and cells that have been disrupted using an ultrasonic crusher can be used as acetic acid ceramides as they are. May be. In addition to the above, the crushing treatment of acetic acid bacteria can be appropriately performed by a conventional method.

  In the case of acetic acid cell disruption product, it is the same as the extract, and the disruption obtained by crushing acetic acid bacteria (usually crushing after adding 0.5 to 2 times the amount of water to the wet cell) The product can be used as an acetic acid ceramide as it is, and various processed products obtained by treating in the same manner as described above can also be used as an acetic acid ceramide.

  The acetic acid bacterium used in the present invention is not particularly limited. Acetobacteria belonging to) are exemplified.

  More specifically, examples of acetic acid bacteria of the genus Gluconacetobacter include Gluconacetobacter hansenii, Gluconacetobacter diazotrophicus, Gluconacetobacter diterotropicus ), Gluconacetobacter sacchari and the like.

  Examples of the genus Gluconobacter include Gluconobacter freatorii and Gluconobacter cerinus.

  Furthermore, the acetic acid bacteria of the genus Acetobacter (Acetobacter tropicalis), Acetobacter indonesiensis, Acetobacter siggi cis (B) ), Acetobacter orientalis, Acetobacter pasteurianus, Acetobacter orenenensis, Acetobacter orenensis Acetobacter lovaniensis), Acetobacter aceti (Acetobacter aceti), such as Acetobacter Pomoramu (Acetobacter pomorum) are exemplified.

  Furthermore, as acetic acid bacteria of the genus Asia (Asia), Asaia bogorensis (Asaia bogorensis), Asaia siemensis (Asiaia siemensis), etc. are illustrated.

  Examples of the acetic acid bacteria belonging to the genus Acidomonas include Acidomonas methanolica.

  As the acetic acid bacteria, in addition to the above, deposited bacteria, commercially available bacteria, acetic acid bacteria contained in vinegar and seed vinegar, and the like can be used as appropriate. Non-limiting examples of deposited bacteria are as follows.

  Acetobacter tropicalis NBRC 16470, A. indonesiensis NBRC 16471, A. sygigi NBRC 16604, S.B. Cis. NBRC 16606, A. pasteurianus DSM 3509, A. orleenensis NBRC 13752, A. lovaniensis NBRC 13753, A. aceti NBRC, A. pomorum) DSM 1 1825 etc.

Gluconacetobacter hansenii NBRC 14820, G. diazotrophicus DSM 5601, G. intermedius DSM 11804, G. sacchar 27
Gluconobacter freatorii NBRC 3264, G. cerinus NBRC 3267, etc.
Asaia Bogorensis NBRC 16594, A. siamensis NBRC 16457, etc.
Acidomonas methanolica DSM 5432 and the like.

  These acetic acid bacteria are used for acetic acid fermentation and grow in large quantities. In addition, acetic acid bacteria have a food experience in Nata de coco, Caspian sea yogurt, black tea mushrooms, etc. Moreover, in vinegar fermentation, it is discarded after fermentation and can be obtained at low cost.

  Examples of the form of the skin function improving composition containing acetic acid ceramide according to the present invention include those formulated into dosage forms such as tablets, capsules, powders, liquids, granules, powders, etc. It can be used as oral cosmetics, supplements and the like. Furthermore, as a form of food and drink, specifically, beverages such as sweets such as candy, gum and jelly, main foods such as bread and cooked rice, beverages such as alcoholic beverages, milk and vinegar beverages, soy sauce, miso And seasonings such as vinegar.

  In the present invention, the proportion of acetic acid ceramide in the food is 0.0001 to 5% by weight, preferably 0.001 to 1% by weight. If the amount of acetic acid ceramide added is less than 0.0001% by weight, cosmetic effects such as moisturizing, rough skin, and prevention of wrinkles are difficult to develop, and if it exceeds 5% by weight, the viscosity during production of the composition increases. Coloring and flavor deterioration occur and are not suitable for oral intake.

  The dosage of acetic acid ceramide as the skin function improving composition of the present invention is 0.01 mg to 1000 mg, preferably 0.1 mg to 100 mg per day for an adult. At doses of less than 0.1 mg per day for adults, there are few cosmetic effects such as moisturizing, improvement of rough skin prevention and improvement of wrinkle prevention, and if it exceeds 100 mg, digestion and absorption may not be possible.

  The composition for improving skin function of the present invention contains acetic acid ceramide as an active ingredient, and after mixing and homogenizing with other various raw materials, a surfactant is mixed as necessary to stabilize the composition. You can also plan. As these surfactants, sorbitan fatty acid ester, glycerin fatty acid ester, propylene glycol fatty acid ester, sucrose fatty acid ester, lecithin and the like can be used.

  In addition, according to the present invention, vinegar and other acetic acid bacteria-containing foods and beverages are crushed, or after adding acetic acid bacteria to food and drink, this is crushed, or acetic acid bacteria crushed products (including the treated products, The same applies hereinafter) to food and drink, or by adding a solvent extract of acetic acid bacterial cells or a crushed processed product thereof (including the processed product, the same shall apply hereinafter) to the food or drink. It can also be manufactured. Therefore, the present invention provides a skin function improving composition with an indication that these crushed processed foods and drinks are orally ingested for skin function improvement. It is clearly distinguished from foods and beverages such as vinegar used for ordinary uses such as mere drinking.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further, this invention is not limited only to an Example.

Example 1
<Anti-wrinkle activity of acetic acid ceramide using human skin organ culture>
Anti-wrinkle activity of acetic acid ceramide was carried out as follows.

  First, acetic acid bacteria ceramide was prepared by the following method. That is, 100 g dry cells of acetic acid bacteria (Acetobacter acetic NBRC 14818) were extracted with 5 liters of a chloroform-methanol solvent (groloform: methanol: water = 1: 1: 0.8) and then separated into two layers. The lower layer was recovered as a crude lipid fraction. Next, the crude lipid fraction was weakly alkali-degraded with 0.4N NaOH to obtain an alkali-stable lipid.

  The obtained alkali-stable lipid is applied to a silica gel column equilibrated with chloroform, washed with a solvent of chloroform: acetic acid = 100: 1, and eluted with a solvent of chloroform: methanol = 97: 3, and the fraction obtained is dried. Solidified to obtain about 0.5 g of acetic acid ceramide.

  As controls, human ceramide (Ceramide: manufactured by Matreya), human sugar ceramide (Ceramide β-D-glucoside from brain: manufactured by Matreya), and plant sugar ceramide (Ceramide β-D-glucoside fluoride) : Made by Matreya).

  Using these samples, the anti-wrinkle activity of acetic acid ceramide was evaluated using the modified Bronoff diffusion chamber according to the anti-wrinkle activity evaluation system shown in (1) to (8) below, and the human-type sugar ceramide, human-type ceramide, Comparative evaluation was made with plant-type sugar ceramide. The modified Bronov diffusion chamber is outlined in FIG. In the figure, PBS represents physiological saline, PG-biocompatible polymer represents polyethylene glycol-biocompatible polymer, Human skin slice represents human skin flakes, and Culture medium represents culture fluid.

(1) A human excised skin piece (with informed consent) was incorporated into a modified Bronov diffusion chamber (schematically shown in FIG. 1). The acetic acid ceramide of the present invention and a control substance were added to the culture medium on the subcutaneous side.
(2) Two hours after adding the acetic acid ceramide and the control substance, the skin pieces were irradiated with ultraviolet A waves (UV-A) at an intensity of ³ J / cm 2 twice per day. Silicate glass was sandwiched between the lamp and the specimen.
(3) Organized skin pieces. As the culture medium, DMEMab medium (+) 10% FBS (fetal bovine serum) was used.
(4) The same treatment was performed every day, and organ culture was performed for 5 to 10 days.
(5) An organ culture trace, a skin piece was isolated from the chamber, and the skin surface was photographed with a stereomicroscope / CCD video camera to obtain a skin surface photograph.
(6) Thereafter, a replica was made with silicon rubber on the skin surface, and a photo of the replica was taken to obtain a replica photo.
(7) A line histogram was created by scanning the surface irregularities of the replica produced in (6) above.

(8) The anti-wrinkle activity is divided into 4 stages of POOR, MEDIUM, GOOD, and EXCELLENT based on the skin surface photograph, replica photograph, and line histogram that projects the uneven shape in the procedure of (1) to (7) above. And evaluated. In addition, when the linear skin groove was short and regular, it was recognized as a texture and was not regarded as wrinkles. In addition, if the skin was wide and thick and long and the shadow was dark, it was recognized as a wrinkle. In the evaluation, POOR is inferior, MEDIUM is normal, GOOD is good, and EXCELLENT is excellent.

  This anti-wrinkle activity evaluation system is excellent in seeing the effects of substances absorbed in the body on the skin, and active ingredients such as ceramide are delivered to the skin through capillaries distributed in the dermis, resulting in stratum corneum ceramide. / Squarene is suitable for investigating the effects of enhancing the moisturizing power of the cell stroma, promoting extracellular matrix construction in the dermis, and promoting fibroblast proliferation.

  In addition, it has been demonstrated that this skin piece survives because it maintains 95% or more of collagen synthesis even when cultured for 4 weeks.

  The human-type ceramide used in the test is a substance in which a saccharide is bound to human-type ceramide, and both have been confirmed to exist in the human body.

In addition, plant sugar ceramide has a structure that is common to plant-derived ceramides, and is characterized by saccharides bound to the ceramide moiety, and has not been shown to exist in human organisms so far. It is considered that a part of each ceramide taken orally is absorbed and acted as it is.
The results are shown in Table 1.

Table 1
――――――――――――――――――――――――――――――――――――――――
Name Evaluation Basis for evaluation ――――――――――――――――――――――――――――――――――――――――
Human sugar ceramide EXCELLENT There is a texture and there are no wide or long wrinkles.
Acetic acid ceramide GOOD There is almost no wide depression. Thin and short wrinkles are scattered, total
Smooth.
Human-type ceramide MEDIUM The skin surface is wrinkled to the extent that there are a few cuticles.
There is almost no, but the surface replica is wide and shaded
The thick skin groove is conspicuous.
Plant-type sugar ceramide MEDIUM Wide and long wrinkles can be seen.
Non-administration zone POOR Wide depressions are observed everywhere.
――――――――――――――――――――――――――――――――――――――――

    As shown in Table 1, acetic acid ceramide has superior anti-wrinkle activity than plant-type sugar ceramide, and from this, acetic acid ceramide has a cosmetic effect such as moisturizing, preventing rough skin, and preventing wrinkle by ingestion. It was confirmed that there is.

(Example 2)
<Anti-wrinkle activity of absorptive ceramide in human skin organ culture>
Part of ceramide taken orally is considered to be decomposed and absorbed.

  Sphingosine (D-erythro-sphingoside: manufactured by Avanti) used for the test is a substance of the sphingoid base part in which the fatty acid and sugar part of the human type ceramide and human type sugar ceramide are decomposed and released.

  Moreover, sphinganine (D-erythro-sphingosine: manufactured by Avanti) is a substance of a sphingoid base part in which the fatty acid part of acetic acid bacteria ceramide is decomposed and released, both of which have been confirmed in vivo.

  The main substance of the sphingoid base moiety in which the fatty acid and sugar moiety of the plant sugar ceramide is decomposed and released is sphingadiene. In the absorption efficiency test in Caco-2, which is a model culture cell of small intestinal epithelial cells, Diene has been shown to be significantly less efficient in absorption than sphingosine (see, eg, “Journal of Nutrition”, 133, p. 2777-2782, 2003).

  Based on the above, the anti-wrinkle activity of human-type sugar ceramide, sphingosine, which is a degradation product of human-type ceramide, and sphinganine, which is a degradation product of acetic acid ceramide, was comparatively evaluated.

The evaluation system used was the same anti-wrinkle activity evaluation system used in Example 1 under the same conditions.
The results are shown in Table 2.

(Table 2)
――――――――――――――――――――――――――――――――――――――――
Name Evaluation Basis for evaluation ――――――――――――――――――――――――――――――――――――――――
Sphinganine EXCELLENT There are almost no wrinkles on the skin surface or surface replica.
Sphingosine GOOD The skin surface has several skin hills, but the surface replica does not have a wide skin groove.
Non-administration zone POOR Wide depressions are observed everywhere.
――――――――――――――――――――――――――――――――――――――――

  From the results of Table 2, it can be expected that acetic acid ceramide exhibits high anti-wrinkle activity even when it is partially decomposed by oral administration, since sphinganine was confirmed to have superior anti-wrinkle activity than sphingosine. From this fact, acetic acid ceramide can be expected to have cosmetic effects such as moisturizing, preventing rough skin, and preventing wrinkles by ingestion.

(Example 3)
<Skin function improvement effect of acetic acid ceramide using atopic dermatitis model mouse>
As a model mouse for atopic dermatitis, 4-week-old NC / Nga Tnd Crj male mice were used. NC / Nga Tnd Crj male mice do not exhibit abnormalities as macroscopic findings in the SPF breeding environment, but strong dermatitis such as rough skin and reduced moisturizing ability in the normal breeding environment in which airborne microorganisms are not controlled It is a mouse that spontaneously develops symptoms.

  2-Hydroxy-C16-Dihydroceramide (manufactured by MATREA) was used as the acetic acid ceramide.

  Two groups, a non-administration control group and an acetic acid ceramide ceramide administration group, were set, and 10 animals each were one group. In the non-administration control group, AIN93G (manufactured by Oriental Yeast Co., Ltd.) was freely ingested. The acetic acid bacteria ceramide administration group was allowed to freely ingest a feed in which 0.15% of acetic acid bacteria ceramide was added to AIN93G.

  22 days after the start of the test, a mouse abdomen shaved and footpad 5 w / v% PiCl solution was applied at 150 μl / animal and sensitized.

  From the 4th day after sensitization, the hair was shaved and the left and right ears (both sides) were applied with a 0.8 w / v% PiCl solution at 150 μl / animal to induce. Administration was carried out for 67 days, and the amount of free drinking was measured once per week, and the amount of water intake per day was calculated. The results are shown in FIG.

  From the results of FIG. 2, the control group and the acetic acid ceramide administration group tend to increase the water intake as the breeding age increases. However, the control group has a greater increase in water intake than the acetic acid ceramide administration group, and the skin It is considered that a large amount of water intake is necessary to replenish the transpiration of water. From this, it can be said that acetic acid ceramide has cosmetic effects such as moisturizing, preventing rough skin, and preventing wrinkle by oral ingestion.

Example 4
<Preparation of tablets>
Acetobacter acetic NBRC 14818 was used as an acetic acid bacterium, and 10 kiloliters of the acetic acid fermentation broth was collected with a high-speed centrifuge (8000 rpm, 20 minutes) to obtain 10 kg of wet cells. 10 kg of the obtained wet microbial cells were washed with distilled water and then freeze-dried under reduced pressure using a large lyophilizer to obtain 1.8 kg of dried microbial cells.

  1 kg of the obtained dried cells were charged into a Soxhlet extractor together with 10 liters of ethanol and heated to reflux for 20 hours. The obtained extract was dried under reduced pressure and dissolved in 5 liters of acetone. The precipitate was removed by filtration, and the filtrate was evaporated to dryness with a rotary evaporator to obtain 1.5 g of light yellowish brown acetic acid ceramide.

  Acetic acid bacteria ceramide 1 g (0.7 wt%), crystalline cellulose 35 g (26.9 wt%), dried corn starch 67 g (51.5 wt%), lactose 22 g (16.9 wt%), calcium stearate 2 g (1.5% by weight) and 3 g (2.3% by weight) of polyvinylpyrrolidone as a binder were added, mixed powdered, and then filled into hard gelatin capsules.

  The tablet thus prepared is sufficiently expected to be usefully orally ingested as a skin function improving composition.

(Example 5)
<Manufacture of jelly>
Acetobacter acetic NBRC 14818 was used as an acetic acid bacterium, and 10 kiloliters of the acetic acid fermentation broth was collected with a high-speed centrifuge (8000 rpm, 20 minutes) to obtain 10 kg of wet cells. The obtained wet bacterial cells (10 kg) were dispersed in an equal amount of distilled water. The dispersed 20 kg of acetic acid bacteria dispersion was passed through a high-pressure homogenizer (20000 psi) three times and subjected to cell destruction treatment, and then freeze-dried under reduced pressure using a large freeze dryer to obtain 1.5 kg of dried cell powder.

  8 g (0.8 wt%) of the obtained dry cell powder, 250 g (25 wt%) sugar, 1.6 g (0.16 wt%) carrageenan, 0.8 g (0.08 wt%) locust bean gum, Xanthan gum 0.8 g (0.08 wt%) was mixed and homogenized to obtain a powder mixture. Weigh 300 g of water in a pan, dissolve 30 g (3.0% by weight) of brown sugar, mix 150 g (15% by weight) of reduced water candy, and stir the powder mixture obtained previously so that it does not become lumpy. Further mixing was performed. They were stirred uniformly and the remaining 258.8 g of water was added and warmed. The solution was heated and dissolved at 85 ° C. for 10 minutes, and then cooled with running water to obtain acetic acid bacterium-containing jelly food.

  The jelly thus prepared can be expected to be effectively orally ingested as a skin function improving composition.

(Example 6)
<Manufacture of beverages>
Acetobacter acetic NBRC 14818 was used as an acetic acid bacterium, and 10 kiloliters of the acetic acid fermentation broth was collected with a high-speed centrifuge (8000 rpm, 20 minutes) to obtain 10 kg of wet cells. 10 kg of the obtained wet bacterial cells were dispersed in 100 liters of vinegar. The dispersed acetic acid bacteria dispersion was passed through a high-pressure homogenizer (20000 psi) three times for cell destruction treatment. The solution was dispensed into 500 ml bottles and sterilized by heating to 75 ° C. to obtain vinegar containing acetic acid bacteria.

  It can be expected that the beverage thus prepared can be effectively taken orally as a composition for improving skin function.

It is a figure which shows the schematic diagram of a modified Bronoff diffusion chamber. It is a figure which shows the skin function improvement effect of acetic acid bacteria ceramide using the atopic dermatitis model mouse.

Claims (9)

  A composition for improving skin function, comprising acetic acid ceramide as an active ingredient.   A composition for improving skin function, comprising a cell lysate of acetic acid bacteria containing acetic acid bacteria ceramide as an active ingredient.   The composition for improving skin function according to claim 1, wherein acetic acid ceramide is extracted from acetic acid bacteria using a polar solvent.   The composition according to claim 1, wherein the composition is formulated into a food or drink, or at least one dosage form for oral consumption of tablets, capsules, powders, granules, powders, and liquids. The composition for improving skin function according to any one of the above.   The composition for improving skin function according to claim 4, wherein the food or drink is vinegar.   The manufacturing method of the composition for skin function improvement characterized by crushing the acetic acid bacteria containing food-drinks.   A method for producing a composition for improving skin function, comprising adding acetic acid bacteria to food and drink and then crushing.   The manufacturing method of the composition for skin function improvement characterized by adding the crushing processed material of an acetic acid microbial cell to food-drinks.   The manufacturing method of the composition for skin function improvement characterized by adding the polar solvent extract of acetic acid microbial cell crushed material to food-drinks.

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