JP2006141252A - Method for producing plant body - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To improve a rooting rate by promoting rooting from a shoot of a plant, and thereby to improve the productivity of clone seedlings. <P>SOLUTION: The method for producing a plant body includes culturing a branch, a stem, an apical bud, an axillary bud, an adventitious bud, a leaf, a seed leaf, a hypocotyl, an adventive embryo, a shoot primordium or the like of the plant by using a medium for culturing a plant tissue to which a silver ion and an antioxidant are added. For example, silver thiosulfate and silver nitrate are used as the silver ion source, and ascorbic acid and a sulfite salt are used as the antioxidant. At the time, the culture is preferably carried out under the irradiation with a light including a component having 650-670 nm wavelength and a component including 450-470 nm wavelength in a ratio of (9:1) to (7:3). <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a method for producing a plant by rooting a plant shoot.

  The step of mass production of homogeneous plants (seedlings) with traits suitable for the purpose is always required when the plant is used industrially for agricultural production, afforestation, breeding and other purposes. . At this time, the traditional cutting method and the tissue culture method born by the development of biotechnology in recent years are useful as a means for mass production of plants. According to these methods, not only can mass production of seedlings be performed, but also plants having the same genetic properties, that is, clone seedlings can be produced in large quantities and rapidly.

  In the cutting method, branches and stems, and in some cases, apical buds, axillary buds, leaves, cotyledons or hypocotyls, etc. are cut out from the individuals of the plant to be proliferated to form cuttings, which are then inserted into a bed or culture medium. Roots to produce plant bodies. On the other hand, when mass-growing a plant by the tissue culture method, an appropriate tissue is cut out from the individual plant to be proliferated, cultured, and expanded from adventitious buds, adventitious embryos, shoot primordia or these tissues. Collect stems and leaves to root. That is, regardless of which method is used to produce cloned seedlings, a process of rooting will be performed.

  Therefore, the rooting ability of plant tissues has a great influence on the productivity of cloned seedlings. In particular, when these clone seedlings are to be used industrially, this is a serious problem for plant species with low rooting ability.

  On the other hand, the involvement of ethylene released by the plant tissue itself is considered as a main factor inhibiting rooting from the plant tissue. Then, in order to suppress generation | occurrence | production of ethylene, when culture | cultivating structures | tissues, such as said adventitious bud, adding silver ion in a culture medium is tried (nonpatent literature 1). However, in this method, although the death rate of the plant tissue is reduced to some extent, the rooting itself is suppressed or delayed, so the effect of improving the rooting rate from the plant tissue is small. Or rather, it was an inhibitory one.

Edwin F. Edwin F. George, “PLANT PROPAGATION by TISSUE CULTURE” UK, 1993, p200

  In view of the above-mentioned problems of the prior art, the present invention promotes rooting from plant tissues and improves the rooting rate thereof, so that clone seedlings, particularly clones belonging to plant species with low rooting ability The aim was to improve the productivity of seedlings, enable mass and rapid production of such cloned seedlings, and open the way to their industrial use.

  As a result of diligent research, the present inventors have found that when a plant shoot is cultured using a medium containing silver ions and an antioxidant, rooting from the shoot is promoted, and the rooting rate is improved. The present invention has been completed.

  That is, the present invention relates to a method for producing a plant body, characterized in that plant shoots are cultured and rooted using a medium to which silver ions and an antioxidant are added.

  According to the present invention, rooting from plant shoots is promoted and the rooting rate is improved, so that the productivity of cloned seedlings by cutting methods and tissue culture methods is improved. Moreover, such an effect is particularly remarkable in plant species with low rooting ability.

  Therefore, the present invention makes it possible to produce a large amount of clonal seedlings quickly even for plant species with low rooting ability, which opens the way for industrial use.

  The present invention can be applied to any plant. However, the present invention can be applied to woody plants having inferior rooting ability compared to herbaceous plants, such as eucalyptus, pine, cherry, acacia, bayberry, cucumber, grapes, apples, roses, camellia, ume, eusloume, jacaranta and the like. More effective when applied. In particular, if the present invention is applied to eucalyptus, pine, cherry, etc., which are known to be difficult to root, great effects can be obtained.

  In the present invention, shoots obtained from these plants are rooted by culturing them in a predetermined medium to produce plants. Here, the shoot means all tissues having rooting ability such as branches, stems, apical buds, buds, adventitious buds, leaves, cotyledons, hypocotyls, adventitious embryos or shoot primordia. Moreover, a plant body means what is obtained by rooting of these structures | tissues, and is used by the meaning including the seedling and the individual | organism | solid which this grew. Adventitious buds can be obtained more efficiently than multi-buds. Multi-buds can be derived by cutting the top buds and buds from the plant to which the present invention is to be applied to produce them, and culturing them using known conditions and known methods. Adventitious shoots can be obtained as individual foliage that elongates from the polyblasts thus induced.

  For example, in order to obtain a shoot used in the present invention by forming a multi-bud from the above-mentioned woody plant, it is generally performed as follows. First, tissues such as apical buds and axillary buds are collected from the plant as a material, and the collected tissues are sodium hypochlorite aqueous solution having an effective chlorine content of 0.5 to 4% or peroxidation having an effective chlorine content of 5 to 15% Surface sterilization is performed by immersing in an aqueous hydrogen solution for 10 to 20 minutes. Next, this is washed with sterilized water, inserted into a solid medium, the buds are opened, and the elongated foliage is subcultured in a medium having the same composition to form a multi-bud. When Eucalyptus or Acacia sprouts are used, the solid medium is 1 to 5% by weight of sucrose, benzyladenine (hereinafter abbreviated as BA) as a plant hormone, 0.02 to 1 mg / l, gellan gum 0.2 A Murashige scoog (hereinafter abbreviated as MS) medium containing ~ 0.3 wt% or agar 0.5-1 wt% or a modified MS medium in which the ammonium nitrate component and the potassium nitrate component of this MS medium are halved is used. preferable. Adventitious buds are actively differentiated from the multi-buds formed in this way and extend. The multiblasts themselves can be maintained and proliferated by appropriately dividing them and culturing them in a medium having the same composition as the medium used for the formation of the multibuds.

  As a medium for culturing and rooting the shoots (hereinafter also simply referred to as a rooting medium), a medium to which silver ions and an antioxidant are added is used. Silver ions may be added to the medium as a silver compound such as silver thiosulfate (hereinafter abbreviated as STS) or silver nitrate. Among them, STS is preferable as a silver ion source used in the present invention, because when this is added to a medium and shoots are cultured, healthy root elongation is promoted. This is presumably because the silver ions derived from STS take the form of silver thiosulfate ions in the medium and are negatively charged. The concentration of silver ions added to the rooting medium is preferably 0.5 μM to 10 μM. Particularly preferably, it is 0.5 μM to 2 μM. When the silver ion concentration in the rooting medium is less than 0.5 μM, the effect can hardly be obtained. When the silver ion concentration exceeds 10 μM, the influence of the silver ion rooting inhibitory effect becomes large, so This is because the root is delayed and the root form is also poor.

  On the other hand, as the antioxidant, for example, known ones such as ascorbic acid and sulfite can be used. Among them, ascorbic acid is preferable as an antioxidant used in the present invention because of its low persistence in the medium. The concentration of the antioxidant added to the rooting medium is preferably 5 to 200 mg / l. Most preferably, it is 5-20 mg / l. When the concentration of the antioxidant in the rooting medium is less than 5 mg / l, almost no effect can be obtained, and when it exceeds 200 mg / l, a tendency to suppress rooting is observed. This is also because there is a risk that the chute will wither.

  In addition to the above components, the rooting medium used in the present invention is generally a known component as a component of a plant tissue culture medium, that is, an inorganic component as an essential component, and a carbon source, vitamins, amino acids as other components. Add plant hormones.

  In this case, as the inorganic component, inorganic salts containing elements such as nitrogen, phosphorus, potassium, sulfur, calcium, magnesium, iron, manganese, zinc, boron, molybdenum, chlorine, iodine, cobalt, etc., for example, potassium nitrate, ammonium nitrate, Ammonium chloride, sodium nitrate, potassium monohydrogen phosphate, sodium dihydrogen phosphate, potassium chloride, magnesium sulfate, ferrous sulfate, ferric sulfate, manganese sulfate, zinc sulfate, copper sulfate, sodium sulfate, calcium chloride, chloride Magnesium, boric acid, molybdenum trioxide, sodium molybdate, potassium iodide, cobalt chloride, and the like can be used. These inorganic salts are preferably added so that the concentration in the rooting medium is about 0.1 μM to about 100 mM, respectively.

  As the carbon source, carbohydrates such as sucrose and derivatives thereof, organic acids such as fatty acids, and / or primary alcohols such as ethanol can be used. These are preferably added to the rooting medium so as to be about 1 to 100 g / l. However, carbon dioxide can also be used as a carbon source. In this case, it is not necessary to add an organic compound such as sucrose to the medium, and it is only necessary to supply carbon dioxide gas to the culture environment so as to be about 300 ppm or more, preferably 1000 ppm or more. Organic compounds such as sucrose can also be used as a carbon source for microorganisms. Therefore, when using a medium supplemented with these, it is necessary to carry out the cultivation in a sterile environment. By using gas as a carbon source, culture in a non-sterile environment becomes possible.

  As vitamins, for example, biotin, thiamine (vitamin B1), pyridoxine (vitamin B4), pyridoxal, pyridoxamine, calcium pantothenate, inositol, nicotinic acid, nicotinic acid amide and / or riboflavin (vitamin B2) are used. Can do. Each of these is preferably added to the rooting medium at about 0.1 to 150 mg / l. As the amino acids, for example, glycine, alanine, glutamic acid, cysteine, phenylalanine and / or lysine can be used, and these can be added to the rooting medium so as to be about 0 to 1000 mg / l, respectively. preferable.

  As plant hormones, for example, auxins and / or cytokinins can be used. Examples of auxins include naphthalene acetic acid (NAA), indole acetic acid (IAA), p-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid (2,4D), indolebutyric acid (IBA) and their derivatives. As benzyladenine (BA), kinetin, zeatin, and derivatives thereof are widely used. These plant hormones are preferably added to the rooting medium so as to be about 0.01 to 10 mg / l, respectively.

  In the present invention, silver ions, antioxidants, carbon sources, and plant hormones are appropriately added to a medium known as a plant tissue culture medium containing inorganic components, vitamins, and amino acids in a predetermined composition. Equally, it may be used as a rooting medium having the above composition. Examples of such a plant tissue culture medium include MS medium, Rinsmeier Skoog medium, white medium, Gamborg B-5 medium, and niche niche medium. Among them, MS medium and Bamboo medium of Gamborg are preferable as the rooting medium of the present invention.

  The rooting medium can be used as a liquid medium, or can be solidified with a solidifying agent such as agar or gellan gum and used as a solid medium. When used as a liquid medium, a porous support made of foamed phenol resin, rock wool, etc., is wetted with this rooting medium and cultivated by inserting the base of the chute and used as a solid medium For example, what is necessary is just to culture | cultivate by inserting the base of a chute | shoot into the thing which added 0.5 to 1weight% agar or 0.2 to 0.3weight% gellan gum to the said rooting medium, and was solidified. . In either case, rooting from the shoot is usually observed in about 2 to 5 weeks.

  Further, in culturing shoots using the rooting medium, irradiation with light containing a wavelength component of 650 to 670 nm and a wavelength component of 450 to 470 nm in a ratio of 9: 1 to 7: 3, preferably 8: 2. It is preferable to carry out below. This is because rooting from shoots is further promoted by culturing by irradiating light containing such wavelength components. The culture temperature is preferably about 23 to 28 ° C.

  After rooting seedlings from shoots are obtained as described above, cultivation is continued for a certain period of time, the roots are enriched, and then transplanted to a seedling container or a nursery, etc. It can be set as a seedling that can be used for a predetermined purpose. Conditions during this period, such as temperature and light intensity when growing seedlings, may be set as appropriate to suit the plant. In addition, when shoots derived from cultured tissues such as adventitious shoots and shoot primordia are rooted, it is usually necessary to undergo an acclimatization process before transplanting to a seedling container or the like.

[Action]
As described above, the silver ions added to the plant culture medium suppress the generation of ethylene and reduce the mortality rate of the cultured tissue. The improvement effect on the rooting rate of the cultured tissue could not be expected, and depending on the plant species and lineage, it could even act as an inhibitor.

  However, when the present inventors added this silver ion together with an antioxidant to a medium and cultured plant shoots using this medium, rooting from the shoots was remarkably promoted and the rooting rate increased. Even if the plant species / lines are expected to decrease in rooting rate due to the action of silver ions added to the medium, not only the silver ion-added medium, but also the silver ion-free medium It was found that the rooting rate from the shoot is improved as compared with.

  Antioxidants added to the medium are thought to promote rooting by acting on the base of the shoots inserted in the medium and suppressing oxidation of cuts and scratches attached to them. Then, the said effect which cancels the inhibitory effect by silver ion and surpasses this cannot be explained. Presumably, the effect of inhibiting the generation of ethylene by silver ions and the effect of inhibiting the oxidation of the shoot base by an antioxidant are combined, and this effect is considered to be achieved.

  Hereinafter, the present invention will be described in more detail with reference to examples.

[Example 1]
Eucalyptus globulus (hereinafter simply abbreviated as E. globulae) line 1 from adventitious buds elongated from 2 to 5 cm in length by using the method described in JP-A-8-228621. ₄ parts diluted by adding STS (AgS ₄ O ₆ ) 2 μM as a silver ion source (2 μM as a silver ion), ascorbic acid 20 mg / l as an antioxidant and IBA 2 mg / l as a plant hormone In a culture chamber adjusted to a carbon dioxide concentration of 1000 ppm, a temperature of 25 ° C., and a humidity of 60%, it was inserted into a porous support made of foamed phenol resin (“Oasis” manufactured by Smithers Oasis) wetted with MS medium. Photosynthesis effective photon flux density 51 including a wavelength component of 450 to 470 nm in a ratio of 8.2: 1.8 It was cultured in the red light under irradiation of 3μmol ^{/ m} 2 ^/ S. At this time, as the culture vessel, a cube having a maximum dimension of 11.5 cm in length, 11.5 cm in width, and 10.0 cm in height and having a slightly protruding body portion was used. The top surface of the culture vessel is provided with two circular openings (each opening has a diameter of 1 cm) to which a polytetrafluoroethylene film ("Milliseal" manufactured by Millipore) having a pore diameter of 0.45 μm is attached. Yes. 25 adventitious buds were inserted per one culture vessel.

  The results are shown in FIGS. 1 and 2 and Table 1. As is apparent from these charts, in this example, rooting from adventitious buds began to be observed on day 13 after the start of culture, and the rooting rate was 60% at the third week after the start of culture. showed that. On the other hand, the death rate was 0% after 3 weeks from the start of the culture.

[Comparative Example 1]
In the same manner as in Example 1, except that STS and ascorbic acid were not added to the medium, Gross adventitious buds were cultured.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds began to be observed from the 14th day after the start of the culture, but at the time of 3 weeks after the start of the culture, the rooting rate was Only 16%, while the death rate was 64% at 3 weeks after the start of the culture.

[Comparative Example 2]
In the same manner as in Example 1 except that no ascorbic acid was added to the medium, Gross adventitious buds were cultured.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds began to be observed on the 15th day after the start of culture, and the rooting rate at the 3rd week after the start of culture was also observed. Only 48%. On the other hand, the death rate was 0% at the time point 3 weeks after the start of culture, as in Example 1.

[Example 2]
Irradiation of adventitious buds with white light irradiation having a photosynthesis effective photon flux density of 68.9 μmol / m ² / S, containing a wavelength component of 650 to 670 nm and a wavelength component of 450 to 470 nm in a ratio of 2.9: 7.1 Except as described below, E.E. Gross adventitious buds were cultured.

  The results after 3 weeks of culture are shown in Table 1. As is clear from Table 1, in this example, the rooting rate of adventitious buds was 32% at the third week after the start of the culture. On the other hand, the death rate was 0% after 3 weeks from the start of the culture.

[Comparative Example 3]
In the same manner as in Example 2, except that STS and ascorbic acid were not added to the medium, E. coli was used. Gross adventitious buds were cultured.

  The results after 3 weeks of culture are shown in Table 1. As is apparent from Table 1, in this example, all adventitious buds died at the time point 3 weeks after the start of the culture, and the death rate was 100%.

[Comparative Example 4]
In the same manner as in Example 2, except that no ascorbic acid was added to the medium, E. coli was used. Gross adventitious buds were cultured.

  The results after 3 weeks of culture are shown in Table 1. As is clear from Table 1, in this example, the rooting rate of adventitious buds at the time of 3 weeks after the start of the culture remained at 24%. On the other hand, as in Example 2, the death rate was 0% even after 3 weeks from the start of the culture.

[Example 3]
E. Similar to Example 1 except that adventitious buds derived from the Globus line 2 were used, and silver nitrate (AgNO ₃ ) 5 μM (also 5 μM as silver ions) was added as a silver ion source instead of STS in the medium. E. Gross adventitious buds were cultured.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds began to be observed from the 12th day after the start of the culture, and at the time of 3 weeks after the start of the culture, the rooting rate was 100%. showed that. On the other hand, the death rate was 0% after 3 weeks from the start of the culture.

[Comparative Example 5]
In the same manner as in Example 3 except that silver nitrate and ascorbic acid were not added to the medium, E. coli was used. Gross adventitious buds were cultured.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds began to be observed from the 12th day after the start of culture, and the rooting rate was 80% at 3 weeks after the start of culture. At this time, the death rate was 12%.

[Comparative Example 6]
In the same manner as in Example 3 except that no ascorbic acid was added to the medium, E. coli was used. Gross adventitious buds were cultured.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds began to be observed on the 15th day after the start of culture, and the rooting rate at the 3rd week after the start of culture was also observed. Only 64%. This is a lower rooting rate than in the case of Comparative Example 5. On the other hand, the death rate was 0% even after 3 weeks from the start of the culture, as in Example 3.

[Example 4]
E. In the same manner as in Example 1, except that adventitious buds derived from Globus line 3 were used, Gross adventitious buds were cultured.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds began to be observed from the 12th day after the start of culture, and the rooting rate was 60% at 3 weeks after the start of culture. showed that. On the other hand, the death rate was 12% after 3 weeks from the start of the culture.

[Comparative Example 7]
In the same manner as in Example 4 except that STS and ascorbic acid were not added to the medium, E. coli was used. Gross adventitious buds were cultured.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds began to be observed from the 12th day after the start of the culture, but at the time of 3 weeks after the start of the culture, the rooting rate was Only 12%, while the death rate was 88% after 3 weeks from the start of the culture.

[Comparative Example 8]
In the same manner as in Example 4 except that ascorbic acid was not added to the medium, Gross adventitious buds were cultured.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds was observed from the 12th day after the start of the culture, but the rooting rate at the 3rd week after the start of the culture was 20%. In addition, the death rate also showed 48% at the time point 3 weeks after the start of the culture.

[Example 5]
Adventitious buds derived from Eucalyptus citriodora (hereinafter simply abbreviated as E. citriodora ) are used, and in the medium, as a silver ion source, 5 μM silver nitrate instead of STS (5 μM as silver ions) Adventitious buds were cultured in the same manner as in Example 1 except that was added.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds began to be observed from the 12th day after the start of the culture, and at 3 weeks after the start of the culture, the rooting rate was 88%. showed that. On the other hand, the death rate was 0% after 3 weeks from the start of the culture.

[Comparative Example 9]
In the same manner as in Example 5, except that silver nitrate and ascorbic acid were not added to the medium, E. coli was used. Citriodora adventitious buds were cultured.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds began to be observed from the 12th day after the start of the culture, and the rooting rate was 64% at the 3rd week after the start of the culture. At this time, the death rate was 12%.

[Comparative Example 10]
In the same manner as in Example 5 except that no ascorbic acid was added to the medium, E. coli was used. Citriodora adventitious buds were cultured.

  The results are shown in FIGS. As is clear from these figures, in this example, rooting from adventitious buds began to be observed from the 12th day after the start of the culture, but the rooting rate at the 3rd week after the start of the culture was 48. % Remained. This is a lower rooting rate than in the case of Comparative Example 9. On the other hand, the death rate was 0% even after 3 weeks from the start of the culture, as in Example 5.

[Example 6]
After collecting the current branches of Yoshino cherry, apple, Yusurame, and Jakaranta belonging to the genus Rosaceae belonging to the family Rosaceae and preparing each 2 to 5 cm in length, the base is STS 2 μM as a silver ion source (2 μM also as silver ions) ), A porous support made of foamed phenolic resin ("Oasis" manufactured by Smithers Oasis) wetted with a 4-fold diluted MS medium supplemented with 10 mg / l ascorbic acid as an antioxidant and 2 mg / l IBA as a plant hormone Insertion and photosynthesis in a culture chamber adjusted to a carbon dioxide concentration of 1000 ppm, a temperature of 25 ° C., and a humidity of 60%, containing a wavelength component of 650-670 nm and a wavelength component of 450-470 nm in a ratio of 8.2: 1.8 Culturing was performed under irradiation with red light having an effective photon flux density of 51.3 μmol / m ² / S. At this time, the same culture vessel as that used in Example 1 was used as the culture vessel. In addition, 25 adventitious buds were inserted per one culture vessel.

  The results after 3 weeks of culture are shown in Table 2. As is clear from Table 2, in this example, the rooting rate was 92% for Yoshino cherry, 80% for apple, 60% for Yusraume, and 76% for Jakaranta at the third week after the start of culture. On the other hand, the death rate was 0% for Yoshino cherry, 4% for apples, 8% for Yusraume, and 4% for Jakaranta.

[Comparative Example 11]
In the same manner as in Example 6 except that STS and ascorbic acid were not added to the medium, E. coli was used. Gross adventitious buds were cultured.

  The results after 3 weeks of culture are shown in Table 2. As is clear from Table 2, in this example, the rooting rate was 60% for Yoshino cherry, 48% for apple, 32% for Yusraume, and 52% for Jakaranta at the third week after the start of culture. On the other hand, the death rate was 20% for Yoshino cherry, 16% for apples, 40% for Yusraume, and 28% for Jakaranta.

[Comparative Example 12]
In the same manner as in Example 6, except that ascorbic acid was not added to the medium, E. coli was used. Gross adventitious buds were cultured.

  The results after 3 weeks of culture are shown in Table 2. As is apparent from Table 2, in this example, the rooting rate was 80% for Yoshino cherry, 60% for apple, 52% for Yusraume, and 64% for Jakaranta at the third week after the start of culture. On the other hand, the death rate was 8% for Yoshino cherry, 4% for apples, 16% for Yusraume, and 4% for Jakaranta.

E. It is a graph which shows a time-dependent change of a rooting rate about the adventitious bud of the Globus line | system | group 1. FIG. E. It is a graph which shows the rooting rate after 3 weeks culture | cultivation about the adventitious bud of the Globus line | system | group 1. FIG. E. It is a graph which shows a time-dependent change of a rooting rate about the adventitious bud of the Globus line | system | group 2. FIG. E. It is a graph which shows the rooting rate after 3 weeks culture | cultivation about the adventitious bud of the Globus line | system | group 2. FIG. E. It is a graph which shows a time-dependent change of a rooting rate about the adventitious bud of the Globus line | system | group 3. FIG. E. It is a graph which shows the rooting rate 3 weeks after culture | cultivation about the adventitious bud of the globula system | strain 3. E. It is a graph which shows a time-dependent change of a rooting rate about the adventitious bud of Citriodora. E. It is a graph which shows the rooting rate after culture | cultivation 3 weeks about adventitious buds of a citriodora.

Claims (3)

A method for producing a plant comprising culturing a plant shoot using a medium to which silver ions and an antioxidant are added and causing the plant to root. The plant shoot is cultivated under light irradiation containing a wavelength component of 650 to 670 nm and a wavelength component of 450 to 470 nm in a ratio of 9: 1 to 7: 3, and rooted. 2. The method for producing a plant according to 1. The method for producing a plant according to claim 1 or 2, wherein a shoot of a woody plant is used as the shoot of the plant.

Images