JP2006136348A - Endotracheal sample administration aid for small animal - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an endotracheal sample administration aid for a small animal, capable of being safely inserted into the oral cavity of the small animal and directly administering a prescribed quantity of a sample to the trachea. <P>SOLUTION: This endotracheal sample administration aid for the small animal is the endotracheal sample administration aid used when the sample is administered from inside the oral cavity of the small animal to the entrance of the trachea. The endotracheal sample administration aid is characterized by a mouth opener in the shape of a truncated cone with both ends opened for keeping the mouth of the small animal in the opened state by inserting a small-diameter end into the mouth of the small animal, a saliva removing means for removing the saliva from inside the oral cavity of the small animal, and a light source for illuminating the inside of the oral cavity of the small animal. The endotracheal sample administration aid for the small animal may also have an enlarged observation means permitting enlarged observation of the entrance of the trachea in the oral cavity of the small animal. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a device capable of easily and accurately administering a sample from the oral cavity for a small animal into the trachea.

  Conventionally, when investigating the effects of inhalation on the trachea and investigating allergic substances targeted by the trachea, small experimental animals weighing less than 100 g, such as mice and hamsters, use chemical substances and proteins in the trachea. A method of administering a sample such as is used. Examples of means for administering a sample into the trachea include means for inhaling a gaseous or mist-like sample from the nose or pharynx. However, depending on the above means, the sample may be absorbed from the skin other than the trachea, ocular mucosa, digestive mucosa, etc., so it is not suitable as a means for accurately evaluating the effects on the trachea. Since it is unclear how much of the sample has reached the trachea, the accuracy is not sufficient for quantitative evaluation.

  In addition, pediatric laryngoscopes and stylets are used to administer samples into the trachea of relatively large laboratory animals such as rats and guinea pigs. It is difficult to use because the instrument is too large for the size of On the other hand, Japanese Patent Application Laid-Open No. 2001-276231 proposes an endotracheal intubation device for small animals as a device used for anesthesia such as a mouse. However, since this device is used in such a manner that the portion of the mouse that contacts the oral cavity is made of metal and pushes the oral cavity in two directions, the mouse is likely to be damaged at the portion in contact with the device. In addition, for the purpose of accurately administering anesthesia, a method of observing the inside of the oral cavity with a small CCD camera has been disclosed, but such a device is expensive and the entire instrument has a complicated structure. was there.

JP 2001-276231 A

An object of the present invention is to provide an endotracheal sample administration aid for small animals that can be safely inserted into the oral cavity of a small animal and can directly administer a predetermined amount of sample to the trachea.
Another object of the present invention is to provide an endotracheal sample administration aid for small animals, which can accurately administer a sample with an inexpensive device in addition to the above characteristics.

  As a result of intensive studies to achieve the above object, the present inventors have developed an auxiliary device having a structure capable of illuminating the bronchial entrance with a light source while keeping the mouth of a small animal open and dehumidifying the mouth. According to the present invention, it was found that the sample can be directly and accurately and quantitatively administered into the trachea of a small animal, and the present invention has been completed.

  That is, the present invention is an endotracheal sample administration assist device used when administering a sample from the oral cavity of a small animal to the bronchial entrance, having a truncated cone shape with both ends open, and a small diameter side end portion of the small animal. An endotracheal sample administration aid for a small animal comprising an opening device that is inserted into the mouth and maintains an open state, saliva removing means for removing saliva from the oral cavity of the small animal, and a light source that illuminates the oral cavity of the small animal Provide equipment. The opening device is preferably formed of an elastic material. The small animal endotracheal sample administration assist device of the present invention may further include a magnifying observation means that enables magnifying observation of the bronchial entrance in the oral cavity of the small animal.

  According to the present invention, a predetermined amount of sample can be directly administered to the bronchial entrance from the small oral cavity of a small laboratory animal, and it is difficult to damage the vicinity of the oral cavity where the device contacts, thereby reducing the burden on the experimental animal. be able to. In addition, since the saliva in the oral cavity is removed by the saliva removing means, the sample can be easily administered. Further, since the narrow oral cavity is illuminated by the light source, the target position such as the bronchial entrance can be accurately positioned. Can be administered.

  Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a front view showing an example of a small animal endotracheal sample administration assist device of the present invention. The small animal endotracheal sample administration assisting device shown in FIG. 1 includes an opening device 1 having a truncated cone shape with both ends open, a body portion 5 and a magnification observation means 4, and a tube pipe 2a and a pump 2b. A saliva removing means 2 configured, and a light source 3 coupled to a battery housing part / grip 6 are provided. As shown in FIG. 2, the magnification observation means 4 fixes the joint portion 4 a included in the magnification observation means 4 to a joint portion 5 a provided at one end of the trunk portion 5, so that the magnification observation means 4 It is installed so as to be able to maintain a partially opened state without completely covering one end of the body part 5. The saliva removing means 2 is inserted from the open part of the trunk 5 on the side where the magnification observation means 4 is provided, and is fixed along the light source 3 disposed near the center in the axial direction of the trunk 5.

  The opening device 1 has a truncated cone shape whose both ends are open. The frustoconical shape with both ends open includes a truncated cone and a truncated cone-like shape, that is, a shape narrowed from the large-diameter side end toward the small-diameter side end. Specific examples of the truncated cone-like shape include a shape in which the small-diameter end portion is tubular, such as a funnel-like shape and a funnel-like shape.

  The opening device 1 is used for the purpose of inserting the small-diameter side end into the mouth of a small animal and maintaining the open state. The small-diameter side end of the opening device 1 is used by being inserted deeply from the mouth of a small animal, for example, to reach the vicinity of the bronchial entrance. For this reason, the maximum diameter (1a in FIG. 3) of the portion (insertion portion: 1b in FIG. 3) of about 1.5 cm from the small-diameter side end of the opening device 1 toward the other end is, for example, 0.3 to 3 cm The thickness is preferably about 0.5 to 2 cm. On the other hand, the maximum diameter (1c in FIG. 3) of the large diameter side end portion of the opening device 1 is, for example, about 1 cm to 5 cm, preferably about 1.5 to 3 cm, depending on the opening diameter of the body portion 5 to be joined.

  It does not specifically limit as a raw material which forms the opening device 1, For example, any of rubber | gum, an elastomer, resin, such as a thermoplastic resin and a thermosetting resin, a metal, etc. can be used. In particular, for the purpose of preventing damage to the oral cavity of a small animal, it is preferable that the part in contact with the oral cavity is formed of a material having elasticity such as rubber or elastomer.

  As the magnifying observation means 4, a conventional device capable of magnifying and observing the oral cavity, particularly the bronchial entrance, can be used, for example, a lens or the like can be used. A plurality of magnification observation means 4 may be installed. For example, a plurality of lenses can be used.

  The trunk | drum 5 is comprised by the cylindrical body with which both ends were open | released, and is used in the form which joined the opening device 1 to the one end, and the expansion observation means 4 to the other end. A concave joint 5a for joining the magnification observation means 4 is provided at the end on the side where the magnification observation means 4 is joined, and the convex joint 4a of the magnification observation means 4 is fitted. . The material which forms the trunk | drum 5 is not specifically limited, The thing of illustration can be used as a raw material of the opening device 1. FIG. The body 5 may be integrated with the opening device 1.

  The saliva removing means 2 has a configuration in which a pump 2b is connected to a tube pipe 2a. The tube pipe 2a is inserted from the side of the instrument body where the magnifying observation means 4 is installed, and the tip is the body of the instrument body. The saliva can be removed by blowing air from the pump 2b through the tube pipe 2a into the oral cavity. The tube pipe 2a is preferably fixed along the light source 3, as shown in FIG. The form of the saliva removing means 2 is not limited to the above example, and is not particularly limited as long as at least a part of the saliva removing means 2 has a form that can be installed inside the trunk 5, and is configured by one or a plurality of members. May be.

  The saliva removing means 2 is not limited to the air blowing means composed of the pump 2b and the tube pipe 2a shown in FIG. 1, and may be air blowing means or suction means having other configurations, and can remove saliva. A conventional method can be used as a simple means. Especially, since a trachea can be open | released simultaneously with the removal of saliva, a ventilation means is preferable at the point which can perform sample administration more easily.

  As the light source 3, a conventional light source can be used. By using the light source 3, it is possible to illuminate the periphery of the bronchus in the back of a narrow oral cavity and reliably administer the sample to the bronchi entrance. The light source 3 is not particularly limited as long as it can be accommodated inside the instrument main body and does not interfere with the sample administration means and the like, and a conventional light source 3 can be used. The light source 3 is coupled to the battery housing portion / grip 7 and penetrates and is fixed to one side of the body 5 near the center.

  The endotracheal sample administration aid for small animals of the present invention is not limited to the configuration shown in FIG. 1, provided that it includes an opening device having a conical shape with both ends open, saliva removing means 2, and a light source. Good. For example, it is also possible for an experimenter or the like to magnify and observe details by attaching a spectacle-type magnifying glass without providing the magnifying observation means 4 in a part of the configuration of the instrument body.

  FIG. 4 is a schematic diagram showing an example of a usage example of the small animal endotracheal sample administration assist device of the present invention. In FIG. 4, the small animal 8 is grasped and fixed by the hand 10 through the holder 9, and the opening device 1 of the small animal endotracheal sample administration aid shown in FIG. 1 is inserted into the mouth of the small animal 8 to maintain the open state. Then, it is shown that the sample is inserted by inserting the instrument into the mouth of the small animal 7 through the sample input means 7. As shown in FIG. 5, the sample insertion means 7 is inserted into the body portion 5 from the side where the enlarged observation means 4 of the instrument body is joined, like the saliva removing means 2. In addition, you may fix a small animal with the fixing tool generally used.

  As the sample administration means 7, for example, a syringe, a dropper or the like can be used.

  The small animal 8 to which the intratracheal sample administration aid is applied means a small animal having a body weight of 100 g or less, and a small animal having a weight of about 15 to 70 g is preferable. Small laboratory animals can be used.

  The sample to be administered may be a liquid or a solid such as a powder. For example, a toxicity test sample, an allergenic evaluation sample such as ovalbumin, an allergy type evaluation sample, a copy toner or dust. Various tests such as a sample for pulmonary inhalation test of foreign substances such as, samples for evaluation; therapeutic agents such as asthma; anesthetics and the like.

  According to the endotracheal sample administration aid for small animals of the present invention, when testing for allergenicity or toxicity, the sample can be directly administered to the bronchus without contacting the mucous membrane or the skin. Can be inspected more accurately. In addition, since the sample does not adhere to the mucous membrane or the like at the time of administration or scatters as a mist and a predetermined amount of sample can be reliably administered to the bronchi, a quantitative test can be performed.

It is a front view which shows an example of the endotracheal sample administration assistance instrument for small animals of this invention. It is the elements on larger scale of the endotracheal sample administration assistance apparatus for small animals of this invention of FIG. It is a front view which shows an example of the opening device which comprises a part of intratracheal sample administration assistance apparatus for small animals of this invention. It is a schematic diagram which shows an example of the usage example of the endotracheal sample administration assistance apparatus for small animals of this invention. It is the elements on larger scale of the usage example of the endotracheal sample administration auxiliary device for small animals of this invention of FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Opening device 1a Maximum diameter of insertion part 1b Insertion part 1c Maximum diameter of large diameter side end part of opening device 2 Saliva removal means 2a Tube pipe 2b Pump 3 Light source 4 Magnification observation means 4a Joining part 5 Trunk part 5a Joining part 6 Battery compartment / gripper 7 Sample administration means 8 Small animal 9 Holder 10 Hand

Claims (3)

An endotracheal sample administration aid used when administering a sample from the oral cavity of a small animal to the bronchial entrance, having a truncated cone shape with both ends open, and opening by opening the small diameter side end into the small animal mouth An intratracheal sample administration assist device for a small animal, comprising: an opening device for maintaining a state; a saliva removing means for removing saliva from the oral cavity of a small animal; and a light source that illuminates the oral cavity of the small animal. The instrument for assisting in administering an intratracheal sample for a small animal according to claim 1, further comprising a magnifying observation means that enables observing the bronchial entrance in the oral cavity of the small animal. The intra-tracheal sample administration assist device for small animals according to claim 1 or 2, wherein the insertion portion is formed of a material having elasticity.

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