JP2006130462A - Functionalized water and production method therefor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide function imparted water which focuses attention on rough similarity among sea water, deep ocean water and a mineral ingredient in the human body, and also focuses attention to the promotion of absorption efficiency of the mineral ingredient to the human body due to hardly digestive sugar, and a production method therefor. <P>SOLUTION: The functionalized water is used together with the hardly digestive sugar 2 and mineral-containing water 1 in which the mineral-containing water is one or not less than one kind selected from the group consisting of natural water 1A, demineralized water 1B separated from sea water B and a demineralized deep layer 1C separated from the deep ocean water C, the hardly digestive sugar is a water soluble starch 2A bonding monosaccharides of three or more, and the water soluble starch is an oligosaccharide 2a; and is any one of a direct use type mixed with the oligosaccharide in a range of 0.1 to 2% and a concentrated type mixing therewith in a range of 2 to 70%. The method for producing the functionalized water comprises primarily mixing the hardly digestive sugar and a part of the mineral-containing water, secondarily mixing the primary mixture 3 and remaining mineral-containing water, and thereafter closely packing the secondary mixture 13 into a vessel 5 through primary filtration, sterilization and secondary filtration. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  TECHNICAL FIELD The present invention relates to functional added water used in daily life, and specifically, drinking water and cooking water composed of an indigestible carbohydrate (oligosaccharide of water-soluble starch) and mineral-containing water having a hardness of 10 or more. It is used as cooking rice water.

When drinking tap water directly or cooking or cooking with it, the sterilization may be caused by the disinfectant remaining in the tap water. Therefore, natural water (also called mineral water) from all over Japan has been sold in plastic bottles for sale, and these can be used for beverages, rice cooking, cooking, hot pots, tea, coffee, etc. It is aimed.
Natural water has a moderate amount of mineral components, so it feels good and is suitable for cooking, tea, etc., but the content of mineral components is small compared to the mineral components that make up the human body, and the content is also small. Therefore, we can hardly expect the mineral supplement effect.

In recent years, it has been clarified that Bifidobacteria is particularly closely related to health among intestinal bacteria, and the use of water-soluble starches of indigestible carbohydrates, especially oligosaccharides, as selective growth saccharides of Bifidobacteria has increased. ing.
Among oligosaccharides, dairy oligosaccharides (aka lactosucrose) are chemically

または

Or

と示される。乳果オリゴ糖は唾液、膵液のα―アミラーゼでは全く分解されず、胃液で１．５％、小腸粘膜酵素で１．６％の僅かな水解が認められる難消化性で、大部分が大腸に到達し、腸内のビフィズス菌を選択的に増加させる。
依って、オリゴ糖の摂取は腸内環境を改善し、糞便中の有機酸の増加、アンモニア、インドール等の腐敗産物の減少をもたらし、排便回数などの便通改善作用が報告されている。そのため、オリゴ糖は「お腹の調子を整える」機能性食品の素材として卓上甘味料、炭酸飲料、キャンディー、ヨーグルト、ゼリー等に配合され、広く用いられている。
特開２００２−２７２４３０ 特開２００２−２１８９５５ 特開２００２−１７３１７

It is shown. Lactose oligosaccharides are not digested at all by α-amylase in saliva and pancreatic juice, and are resistant to digestion with slight hydrolysis of 1.5% in gastric juice and 1.6% in small intestinal mucosal enzymes, and most of them are in the large intestine. Reach and selectively increase the intestinal bifidobacteria.
Therefore, ingestion of oligosaccharides has improved the intestinal environment, leading to an increase in organic acids in feces, and a decrease in spoilage products such as ammonia and indole, and a bowel movement improving effect such as the number of defecations has been reported. For this reason, oligosaccharides are widely used as ingredients for functional foods that “tune the stomach” in tabletop sweeteners, carbonated beverages, candy, yogurt, jelly, and the like.
JP2002-272430 JP 2002-218955 A JP 2002-17317 A

Natural water with a hardness of 10 or more is delicious because it contains a moderate amount of mineral components, but the type of minerals contained is small compared to the mineral components that make up the human body, and we cannot expect much absorption efficiency from the intestinal tract. It is thought that the mineral supplementation effect is scarce.
Therefore, the present invention has been made in view of such problems of the prior art, and the object of the present invention is that about 80% of the human body is water, and the mineral components of seawater and deep sea water are Focusing on the fact that it is almost the same as the mineral components that make up the human body, the water-soluble starch of indigestible saccharides promotes the absorption efficiency of mineral components to the human body, etc., and new functionalities using these It came to develop additional water and its manufacturing method.

In order to achieve the above-mentioned object, the functional additional water of the present invention is characterized in that it comprises, as claimed in claim 1, an indigestible saccharide and mineral-containing water having a hardness of 10 or more.
Claim 2 is the functional additional water of claim 1, wherein mineral-containing water is separated from natural water pumped from the ground, demineralized water obtained by separation from seawater, and deep sea water 200 meters or less below sea level. It is one kind selected from the desalted deep water obtained in the above.
Claim 3 is the functional additional water according to claim 1, wherein mineral-containing water is separated from natural water pumped from the ground, demineralized water obtained by separation from seawater, and deep sea water 200 meters or less below sea level. It is characterized by being 2 or more types selected from the obtained desalted deep water.

Here, the functional additional water means all of the water containing many kinds of mineral components beneficial to the human body, and is used not only for drinking water but also for cooking water used for cooking food and for cooking rice. Cooked rice water is also included.
Here, natural water refers to water that has a large amount of mineral components, such as spring water that springs on land, groundwater that is pumped from the ground on land, and mineral water that is currently on the market, and desalted water is reverse osmosis from seawater. Desalted using a membrane, desalted using an ion exchange membrane, desalted by electrolysis, desalted by the multistage electrodialysis method previously invented by the applicant, etc. In other words, desalted deep water is one that has been desalted using reverse osmosis membranes from deep sea water, one that has been desalted using an ion exchange membrane, one that has been desalted by electrolysis, and the applicant first invented. This refers to those desalted by the multistage electrodialysis method.

Claim 4 is the functional additional water of claims 2 and 3, wherein the desalted water and the desalted deep water are separated by fresh water and mineral concentrated water separated by a multistage electrodialysis method, and separated by a multistage electrodialysis method. It is characterized in that it is at least one selected from bittern obtained from the biconcentrated brine further through dehydration separation means.
According to a fifth aspect of the present invention, the functionally added water according to any one of the first, second, third and fourth aspects is characterized in that the hardly digestible saccharide is water-soluble starch in which three or more monosaccharides are bound.
Claim 6 is the functional addition water according to claim 5, wherein the water-soluble starch is an oligosaccharide, and the oligosaccharide is mixed in the range of 0.1 to 10%. And
A seventh aspect of the invention is characterized in that in the functional addition water according to the fifth aspect, the water-soluble starch is an oligosaccharide and the oligosaccharide is mixed in a range of 2 to 70%.

Here, the multistage electrodialysis method means that seawater or deep ocean water is separated into fresh water and first concentrated brine by the first treatment device, and the first concentrated salt water is separated from the mineral concentrated water by the second treatment device. This refers to separation into concentrated salt water, and mineral concentrated water contains a balance of mineral components substantially the same as those of the human body in a well-balanced manner. The dehydration / separation means is means for heating, filtering, dehydrating, drying, etc. the second concentrated salt water to obtain bittern and salt. Since bitumen contains many kinds of mineral components, particularly trace minerals, this is determined at a predetermined magnification. It can also be diluted with

Here, the indigestible carbohydrate has no adverse effects on the human body, and has an effect of improving the absorption efficiency of minerals to the human body or improving constipation, such as water-soluble starch and low molecular weight dextrin. Among them, water-soluble starch refers to oligosaccharides, and oligosaccharides reach the large intestine without being absorbed in the digestive tract, undergo fermentation by intestinal bacteria, acetic acid, butyric acid, propionic acid, etc. Refers to indigestible oligosaccharides that are absorbed as short-chain fatty acids and become energy, xylooligosaccharides, lactulose (milk oligosaccharides), fructooligosaccharides, soybean oligosaccharides, isomaloulgigosaccharides, dairy oligosaccharides, fructooligosaccharides, raffinose Etc.
The range that can be adopted as the blending amount of oligosaccharide (mainly dairy oligosaccharide) is 0.5 to 50 g per 500 ml, and if the blending amount is less than 0.5 g, the effect tends to be dilute. If it exceeds, the taste tends to be impaired.

The functional additional water according to claim 8 is characterized in that the mineral-containing water and the hardly digestible carbohydrate are separated and stored, and the mineral-containing water and the hardly digestible carbohydrate are mixed and used at the time of use.
For example, the first container contains mineral-containing water, the second container contains non-digestible carbohydrates, and the container body contains mineral-containing water. Say to contain carbohydrates, open the first container at the time of use, put the indigestible sugar of the second container into the first container, or break the inner plug body to prevent the indigestible sugar It refers to the use of a mixture of mineral-containing water and non-digestible saccharide in a container body.
If the first container and the second container are detachably fitted, it is possible to prevent the first container and the second container from being dispersed. A lid-mounted type that breaks down when pressed and drops indigestible carbohydrates, and is placed on the mouth of the main body, suspended in the mouth of the container, and ruptured by the screwing of the lid with a needle. There is a mouth locking type that drops a sex carbohydrate.

In the method for producing functional added water according to the present invention, as claimed in claim 9, the hardly digestible saccharide and a part of the mineral-containing water are primarily mixed, and the primary mixture and the remaining mineral-containing water are secondarily mixed. Then, the secondary mixture is subjected to primary filtration, sterilization, and secondary filtration, and the container is hermetically filled.
Here, primary mixing refers to mixing a part of the total mixing amount in advance, and secondary mixing refers to mixing the remainder into the primary mixture, and mixing in two portions to mix the lump. Eliminates generation and enables even mixing.
Here, sterilization refers to sterilization at a high temperature of 100 ° C. or higher for 2 to 10 seconds.
After the functional additional water of the present invention is hermetically filled in a container, the product is shipped after cooling, label shrinkage, appearance inspection, shelf life printing, box making, exterior printing, calibration, and exterior inspection.

Since the functional addition water of this invention is as above-mentioned, there exists an effect described below.
Since the functional additional water of claim 1 has mineral-containing water, it can be used for cooking, tea, coffee, etc. as normal water, and it can be expected to have the functionality of supplementing trace minerals that tend to be insufficient. Moreover, because it uses indigestible carbohydrates, it helps to eliminate constipation.
In addition to the characteristics of claim 1, the functional additional water of claim 2 can soften the mouth feel and improve the taste when natural water is used as the mineral-containing water. In addition, when desalted water is used, seawater contains many types of mineral components different from natural water, particularly trace minerals that are difficult to replenish from other sources, which is beneficial for supplementing trace minerals. Further, when desalted deep water is used, the desalted deep water substantially contains mineral components constituting the human body, and since it is highly clean at low temperatures throughout the year, it is useful as a stable raw material.
In addition to the features of claim 1, the functional additional water of claim 3 is natural water and desalted water, natural water and desalted deep water, desalted water and desalted deep water, natural water and desalted water as mineral-containing water. By selecting the water and desalted deep water, the mineral components can be blended in a well-balanced manner or the hardness can be adjusted. Moreover, functional drinks suitable for the purpose, for example, drinks suitable for sports, drinks suitable for coffee, drinks suitable for cooking rice, drinks suitable for infants, and the like are possible by combining these.
In addition to the features of Claims 2 and 3, the functionally added water of Claim 4 uses mineral concentrated water separated by a multistage electrodialysis method. Therefore, the effect of replenishing the shortage of trace minerals is expected. When bittern is used, trace minerals can be added in a small amount.

In addition to the features of Claims 1, 2, 3, and 4, the functional added water of Claim 5 uses water-soluble starch combined with 3 or more monosaccharides as indigestible carbohydrates. Contributes to the improvement of flora (intestinal flora). That is, it helps to increase so-called good bacteria such as bifidobacteria and decrease so-called bad bacteria such as Clostridium perfringens.
In addition to the features of claim 5, the functionally added water of claim 6 is characterized in that the water-soluble starch is an oligosaccharide, and this oligosaccharide (mainly dairy oligosaccharide) is mixed in the range of 0.1 to 10%. Because it is a direct use type, it can be used as it is for beverages or for cooking such as cooked rice or boiled food.
In addition to the characteristics of claim 5, the functional added water of claim 7 is a concentrated type in which the water-soluble starch is an oligosaccharide and the oligosaccharide is mixed in an amount of 2 to 70%. Can be used.

  The functional added water according to claim 8 separates and contains mineral-containing water and indigestible saccharides. Therefore, the indigestible saccharide is added to the mineral-containing water during use, that is, mixed before use. Can do.

Since the manufacturing method of the functional addition water by this invention is as the claim 9, there exists an effect described below.
Since the indigestible saccharide and the mineral-containing water are mixed twice without mixing at one time, the lump of the indigestible saccharide can be prevented and uniform mixing can be achieved.
In addition, since the secondary mixture is filled by primary filtration, sterilization, and secondary filtration, it is hygienic with no contamination.

  If the best form of the functional addition water by this invention is demonstrated in detail based on FIG. 1, it will consist of at least the non-digestible saccharide 2 and the mineral containing water 1 of hardness 10 or more, 1A of natural water as the mineral containing 1, Demineralized deep water 1C obtained by separating from deep sea water C pumped from 200m deep below sea level, specifically mineral water 1a pumped from underground as natural water 1A As a water-soluble starch 2A in which 3 or more monosaccharides are bound, oligosaccharide (milk oligosaccharide) 2a is used, and its mineral water 1a, desalted deep water 1C and oligosaccharide 2a are converted into mineral water 1a> desalted. It is a mixture of deep water 1C> oligosaccharide 2a.

As desalted deep water 1C, fresh water 4T and mineral concentrated water 4V obtained by separation by the multistage electrodialysis method 10 previously invented by the applicant, and second concentrated salt water 4W separated by the multistage electrodialysis method 10 are used. Furthermore, at least one kind of bittern 4X obtained through the dehydration separation means 20 is blended.
As shown in FIG. 2-1, the multistage electrodialysis method 10 separates the deep ocean water C into the first first condensed salt water 4U and the fresh water 4T by the first treatment device 11 using the ion exchange membrane M1. In the second treatment device 12 using the ion exchange membrane M2 having excellent selectivity, the first concentrated salt water 4U separated by the first treatment device 11 is concentrated with monovalent chlorine ions, sodium ions, and the like. Separated into concentrated salt water 4W and mineral concentrated water 4V composed mainly of useful trace minerals, polyvalent ion minerals obtained by removing monovalent ions.
As shown in FIG. 2-2, the dehydration separation means 20 includes a first heating step, a filtration step, a second heating step, a centrifugal dehydration step, and a drying step. To get.

If the 1st embodiment of the functional addition water by this invention is described about the point which is different from the best form, the functional addition water of 1st embodiment will use the oligosaccharide 2a as the water-soluble starch 2A, and the mineral containing 1 Only mineral water 1a of natural water 1A is used, and they are mixed in a ratio of mineral water 1a> oligosaccharide 2a.
The range that can be adopted as the blending amount of oligosaccharide 2a is 0.5 to 25 g per 500 ml, the preferred blending amount is 1 to 10 g per 500 ml, the optimum blending amount is 2 to 4 g per 500 ml, and the blending amount is per 500 ml. When the amount is less than 1 g, the effect is hardly exhibited, and when the blending amount exceeds 10 g per 500 ml, symptoms such as diarrhea and the like tend to be impaired.

If the 2nd embodiment of the functional addition water by this invention is described about a different point from the first embodiment, the functional addition water of the second embodiment will be demineralized water separated from the seawater B as mineral-containing 1. Only 1B is used, and the desalted water 1B and oligosaccharide 2a are mixed in a ratio of desalted water 1B> oligosaccharide 2a.
As demineralized water 1B, fresh water 3T and mineral concentrated water 3V separated by multistage electrodialysis method 10 and second concentrated salt water 3W separated by multistage electrodialysis method 10 were further obtained through dehydration separation means 20 Can be divided into a direct use type and a concentrated type by a combination of fresh water 3T, mineral concentrated water 3V, and bittern 3X.
The concentration of oligosaccharide 2a can be adjusted to 2 to 70%, a preferable concentration of 5 to 50%, and an optimal concentration of 10 to 30%.

If the third embodiment of the functional additional water according to the present invention is described with respect to the points different from the first and second embodiments, the functional additional water of the third embodiment is mineral-containing 1, deep ocean water C Only the desalted deep water 1C obtained by further separation is used, and the desalted deep water 1C and the oligosaccharide 2a are mixed at a ratio of the desalted deep water 1C> the oligosaccharide 2a.
As the desalted deep water 1C, fresh water 4T and mineral concentrated water 4V separated by the multistage electrodialysis method 10 and second concentrated salt water 4W separated by the multistage electrodialysis method 10 were further obtained via the dehydration separation means 20. At least one kind of bittern 4X is blended.

If 4th embodiment of the functional addition water by this invention is described about a different point from 1st thru | or 3rd embodiment, the functional addition water of 4th embodiment is the mineral of natural water 1A as mineral containing 1 Water 1a and demineralized water 1B separated from seawater B are used, that is, containing mineral water 1a of terrestrial mineral and demineralized water 1B of marine mineral, and the mineral water 1a and demineralized water 1B. And oligosaccharide 2a are mixed in a ratio of mineral water 1a> demineralized water 1B> oligosaccharide 2a.
When mineral concentrated water 3V separated from seawater B is used as demineralized water 1B, there are more types of mineral components than natural water 1A, and accordingly, it is suitable for replenishment of minerals. Furthermore, when using bittern 3X, trace minerals are concentrated, so only a small amount can be added.

If the fifth embodiment of the functional additional water according to the present invention is described in terms of differences from the first to fourth embodiments, the functional additional water of the fifth embodiment is separated from the seawater B as mineral-containing 1. Demineralized water 1B and demineralized deep water 1C obtained by separation from deep ocean water C are used. Demineralized water 1B, demineralized deep water 1C and oligosaccharide 2a are converted into demineralized water 1B + demineralized water. It is a mixture of deep water 1C> oligosaccharide 2a.
When mineral concentrated water 4V separated from ocean deep water C is used as desalted deep water 1C, there are more types of mineral components than natural water 1A. Furthermore, when using bittern 4X, trace minerals are concentrated, so only a small amount can be added.

If the sixth embodiment of the functional added water according to the present invention is described in terms of differences from the first to fifth embodiments, the functional added water of the sixth embodiment is a mineral containing 1A of natural water as a mineral-containing one. Water 1a, demineralized water 1B separated from seawater B, and demineralized deep water 1C obtained by separating from deep seawater C, that is, containing land minerals and marine minerals The mineral water 1a, the desalted water 1B, the desalted deep water 1C, and the oligosaccharide 2a are mixed in a ratio of mineral water 1a> desalted water 1B + desalted deep water 1C> oligosaccharide 2a.
The desalted water 1B and the desalted deep water 1C are substantially the same in the types of mineral components and their contents, but the desalted deep water 1C is separated from the ocean deep water C, so it is highly clean. The ingredients are also stable.

Referring to FIG. 4A, the first classified accommodation form of the functional additional water according to the present invention will be described. The mineral-containing water 1 is contained in the first container 15 and the indigestible carbohydrate 2 is contained and sealed in the second container 25. At the time of use, the first container 15 is opened, the indigestible carbohydrate 2 in the second container 25 is put into the first container 15, and the mineral-containing water 1 and the indigestible carbohydrate 2 are mixed. Use.
As a specific example of the first packaging form, in the first container 15 as shown in FIG. 4B, the fitting part 19 that opens outwardly to a part of the container body trunk part 16a is provided. The part 26a is provided with a fitted part 29 that engages with the fitting part 19, and the second container 25 is removably engaged with the first container 15, or the first part as shown in FIG. The connecting part 14 is provided in the lid 17 of the container 15, the connected part 24 is provided in the lid 27 of the second container 25, and the connected part 24 is connected to the connecting part 14.

When the 2nd classification accommodation form of the functional addition water by this invention is demonstrated, the container main body 6 which accommodates the mineral containing water 1, the lid body 7 which closes the main body mouth part 6b, and the indigestible carbohydrate 2 are accommodated. It consists of a stopper 8, and the indigestible saccharide 2 of the middle stopper 8 is put into the container body 6 at the time of use, and the mineral-containing water 1 and the indigestible saccharide 2 are mixed and used.
In the second packaging form, as shown in FIGS. 5-1 to 5-3, the lid body 7 has a pressing portion 7c that can be pressed and deformed to the top plate 7a, and the inner plug body 8 has a breaking portion 8c at least below the storage portion 8a. Is used, and the pressing portion 7c of the lid top plate 7a is pressed and deformed during use, pressure is applied to the inner plug 8, the breaking portion 8c of the storage portion 8a is broken, and the indigestible carbohydrate 2 is dropped. 5-2, the lid body 7 is provided with an auxiliary wall 37 that can be cut off on the lower side of the peripheral wall 7b, and a needle portion 47 that hangs down on the top plate 7a, and the inner plug body 8 is inserted into the main body mouth portion 6b. A storage portion 8a and a flange portion 8b placed on the lip of the main body are provided. When the auxiliary wall 37 of the lid body 7 is removed during use, and the lid body 7 is screwed, the needle portion 47 becomes the inner plug body 8. The ingestible carbohydrate 2 is dropped through the storage portion 8a.
In any case, there is an advantage that the mineral-containing water 1 and the hardly digestible carbohydrate 2 can be mixed without opening the container 5.

The method for producing functional added water according to the present invention will be described with reference to FIG. 6. First, the indigestible carbohydrate 2 and a part of the mineral-containing water 1 are mixed, and the primary mixture 3 and the remaining mineral-containing water 1 are mixed. After the secondary mixing, the secondary mixture 13 is subjected to primary filtration, sterilization, and secondary filtration to fill the container body 6, and the container opening 6 b is sealed with the lid 7.
Next, put it in a bottle cooler, shrink the label on the outside of the container 5, visually inspect it, print the expiration date and unique symbol etc. on the thing that passed the inspection, house this in a packaging box, box it, packaging box The product is printed on the outer surface and shipped after calibration and inspection.

（２）ミネラル濃縮水Ｖ；海洋深層水Ｃを多段式電気透析法１０により分離して得たものであり、ミネラル成分は図３の通りである。
（３）オリゴ糖２ａ；株式会社横浜国際バイオ研究所の乳果オリゴ糖ＬＳ−９０ Experimental Example 1 Sample Material (1) Mineral Water 1a; “Alps Semen” manufactured and sold by the applicant
The mineral components contained in the Alps semen are as follows.

(2) Mineral concentrated water V; obtained by separating the deep sea water C by the multistage electrodialysis method 10, and the mineral components are as shown in FIG.
(3) Oligosaccharide 2a; dairy oligosaccharide LS-90 from Yokohama International Bio Institute

2. Test sample Components of sample material when oligosaccharide (milk oligosaccharide) 2a is blended at a ratio of about 0.7% and the hardness is adjusted to 250.

As an effect of oligosaccharide 2a,
A, Indigestible, low-energy property It is not decomposed and absorbed in the stomach and small intestine, reaches the large intestine, undergoes fermentation by some intestinal bacteria, and becomes short-chain fatty acids such as acetic acid, butyric acid, propionic acid, etc. from the colonic mucosa It is absorbed and becomes an energy source .
B, Intestinal regulating action After reaching the large intestine without being absorbed in the stomach and small intestine, it becomes a growth factor such as bifidobacteria to improve the intestinal flora and prepare the intestinal environment.
C, Promotion of mineral absorption Short-chain fatty acids lower the intestinal pH and promote absorption of calcium, iron, magnesium and the like.

Specifically, we developed a 500ml PET bottle type water supplemented with LS of the present invention, and the effect on the bowel movement of healthy female students of 3.0g per day as dairy oligosaccharide 2a when ingested for 14 days.・ Studies were made based on the amount, stool color, shape, hardness, sensation after defecation, odor, abdominal symptoms, and effects on gut microbiota.
Furthermore, the influence of the safety of ingesting twice and three times the normal dose of LS-blended additional water on mud / watery fecal excretion frequency and abdominal symptoms was investigated.

Test method 1. Subjects Questionnaires regarding fecal bowel were conducted in advance for healthy female students attending medical colleges in Aichi Prefecture. Among these, 92 subjects who understood the main points of this study and obtained their consent were used as subjects.
In the test, 92 female students were randomly divided into a group of 60, 12 and 20 subjects, and the following three tests were conducted.
In the case of test 1, normal dose intake test n = 60 with LS-containing added water, mean age 20.7 ± 0.6 years,
In the case of Test 2, the fecal flora search test is n = 12, the average age is 20.6 ± 0.5 years, and both tests 1 and 2 are divided into 2 groups, 1st group and 2nd group, single blind crossover A test was conducted.
In case of Test 3, increase test of LS-blended additional water n = 20, average age 19.8 ± 0.5 years, giving 2 and 3 times the normal dose of LS-blended additional water for 7 days, The effect on the frequency of excretion of watery feces and abdominal symptoms was observed.
In addition, this test will comply with the spirit of the “Helsinki Declaration”, the test content will be fully explained to the subject beforehand, and written consent will be obtained from the university ethics committee to which the student belongs. And under the supervision of a doctor.

2. Test addition water Two types of LS-mixed addition water and placebo addition water were used in the main test. 75% dairy oligosaccharide liquid LS-90L in LS blended water: 90% or more of dairy oligosaccharide 2a in solid components, 4.45g in 500 ml each made by Shimizu Minato Sugar Co., Ltd. did. Since there was almost no sweetness in this compounding quantity, no substance was mix | blended with the placebo addition water.
Additive water is a trade name in which mineral water 1a is added to purified mineral concentrate V after removing monovalent salt NaCl and KCl from deep seawater C collected from depth 321m in Toyama Bay, Japan Sea. "Sea mineral water" refers to the applicant's product.
Table 1 shows the composition of the LS-blended additional water and the placebo-added water.

3. Test Schedule The test schedule is shown in FIGS. 7-1 and 7-2.
Test 1 = Regular dose intake test for test additional water The test was conducted by the single blind crossover method. After the 1-week pre-observation period, the first intake period of 2 weeks for LS-containing supplementary water or placebo-added water, the rest period of 2 weeks, and the second intake period of LS-added supplementary water or placebo-added water for two weeks Carried out.

Test 2 = Fecal microbiota search test Each group of 6 subjects was tested in the same manner as in Test 1, and during the 7-week test period, the middle of the previous observation period and the first intake period Fecal samples were provided for a total of 4 times in the second half and the rest period and the second half of the second intake period.
In Tests 1 and 2, the subjects in Group 1 and Group 2 crossed over LS-containing water and placebo-added water during the intake period.
In addition, only the daily intake was prescribed at the time of intake, and the intake time and the amount taken at one time were not specified.

Test 3 = Increased intake test of LS-blended additional water 6 subjects were given LS-blended additional water 3.0 g as 500 ml whey oligosaccharide 2a per day, Phase A, 2 times 1000 ml whey oligosaccharide 2a 0.0 g, C period, 3 times the amount of 1,500 ml whey oligosaccharide 2a was gradually increased to 9.0 g, E period, and ingested for 7 days each.
In order to remove the influence of intake of dairy oligosaccharide 2a, after the end of the A period and the C period, the F period was provided as the observation period in the week following the 7-day rest period B, D and E periods.
In addition, during the test period, we instructed to avoid as much as possible the intake of foods reinforced with oligosaccharides, dietary fiber, sugar alcohol, etc., food natto containing live bacteria, lactic acid bacteria preparations, etc., and constipation drugs and antibiotics.

4). Survey Items The defecation status, stool properties, and abdominal symptoms in tests 1, 2, and 3 were surveyed using a questionnaire system.
The survey items were the day when each test period was color-coded for the presence of stool and the time of stool, the amount of stool, the color of the stool, the shape of the stool, the smell of the stool, the hardness of the stool, the sensation after the stool and the occurrence of abdominal symptoms A one-page survey diary was prepared and entered by each subject.
The amount of defecation was recorded as a unit number of L-sized eggs having a diameter of 3.5 cm, a length of 5 cm, and a weight of about 50 g.
There are three stool colors: yellowish brown and brown “yellow”, brown and dark brown “brown”, and blackish brown “black”.
There are three types of stool shapes: “Tick / Corokoro”, “Banana / Semi-kneaded”, and “Mud / Water”. "Indicator card"
There are three levels of stool hardness: “soft”, “normal” and “hard”.
There are three levels of stool smell: “I do n’t care”, “Normal” and “Kusai”.
The sensation after defecation was evaluated based on three levels: “refreshing”, “normal”, and “feeling residual stool”.
Abdominal symptoms were “good”, “abdominal pain”, “screaming belly”, “fullness of stomach”, “farting” We selected from "I felt painful feeling belly", "I was nauseous", and "Other", and in the case of "Other", I was asked to enter specific symptoms.
Furthermore, in the remarks column, items such as the remaining amount of test food intake, overeating, medication use and poor physical condition were entered briefly.

5. Analysis of faecal flora Feces were collected immediately after the stool so that the sample was as homogeneous as possible. To the laboratory. One of them was aseptically added with an anaerobic diluent and then homogenized with a Stomacker 80T Organo Co., Ltd. at 230 rpm for 30 seconds.
Intestinal bacteria were separated by partially changing the method of Mitsuoka et al., And the target of measurement was total anaerobic bacteria, Bifidobacterium Bifidobacterium, lecithinase-positive Clostridium sp, and Escherichia coli Escherichia coli. Bifidobacteria detection medium: 50% V / V equine defibrillated BL agar medium, lecithinase positive detection medium: NN medium Nissui Pharmaceutical Co., Ltd., E. coli group detection medium: 70 mocult ES medium Merck stock Made by company.
In addition, the anaerobic culture method used both the steel wool method and the anero-pack kenki system Mitsubishi Gas Chemical Co., Ltd. oxygen absorption and a carbon dioxide generator. Bifidobacteria were isolated from the colonies that appeared on the BL medium used as the medium for detecting Bifidobacterium during each test period, purely cultured, and differentiated in accordance with Mitsuoka's method. Biological identification was performed by a simple identification kit “Api 50CHL” manufactured by Bio Merieux.

6). Measurement of Fecal Distilled Water Dilution pH and Ammonia A stool sample from one stool collection tube was accurately weighed in a range of 0.1 to 0.5 g into a 15-ml test tube with a scruff cap. After adding 15 times the amount of distilled water to the fecal weight and stirring and dissolving, a pH electrode was inserted into the supernatant, and the pH was measured with a pH meter 704, manufactured by Metrohm Shibata Corporation.
The fecal solution was centrifuged at 3,000 rpm for 10 minutes, and the supernatant was used as an ammonia measurement sample. The amount of ammonia in the stool was measured using an ammonia measurement kit with a deproteinizing reagent “Ammonia Test Wako” manufactured by Wako Pure Chemical Industries, Ltd., and colorimetric determination was performed using a HITACHI U-1,000 Spectro pH otometer.

7). Measurement of stool water content The weight of the stool collection tube containing the remaining stool was accurately measured with a direct chemical balance, frozen at -80 ° C, and then dried for 48 hours or more using a freeze dryer RFS2000B, USA. Turned into. Thereafter, the weight of each stool collection tube was measured to determine the weight of the stool powder, and the moisture weight and moisture content (%, 25 ° C., 60 to 70% humidity) were calculated.

8). Statistical processing Regarding the number of defecation days, number of defecations, and amount of defecation, first test the significant difference between each test period for all subjects using the Wilcockson signed rank sum test, then the number of defecations in the previous observation period However, a stratified analysis was performed by dividing addictive constipation 3 times or less per week, 4-7 times constipation tendency person and 8 or more non-constipation people.
The occupancy of bifidobacteria relative to the total number of anaerobic bacteria was tested using the Wilcockson signed rank sum test. Regarding the defecation status, fecal characteristics, and abdominal symptoms, the frequency of appearance in each test period was determined, and χ ² test was performed for the independence of the distribution.
The bifidobacteria count, pH, ammonia amount, and moisture content% were tested by the Wilcoxon signed rank sum test. All statistical processes set the significance level to 5%, and stat cells were used as statistical software.

Results Effects on Defecation Days, Number of Defecations, and Defecation Volumes The effects of LS-containing supplementary water on the defecation of all 60 subjects for one week in the pre-observation period, one week before and during the intake period of placebo supplemented water, two weeks each The period before and after the rest period, the same period, and the period before and after the intake period of the LS-containing added water were compared with each other.
Next, the average number of defecations of addictive constipation of 3 or less per week in the previous observation period was 2.42 times, average of 4.97 times of 19 people and 4 to 7 times of constipation, 30 times and 8 times or more The non-constipated average was 9.00 times, and the data was divided into three groups of 11 people.
These results are shown in Table 2.

In comparison of the average defecation days in one week for all subjects, the pre-observation period was 4.22 days, the placebo-supplemented water intake period was 4.22 days, 4.23 days, and the rest period was 4 days. 15 days and 4.10 days, on the other hand, the number of days before and after the intake period of LS-containing added water was 5.02 days and 4.68 days, indicating an increase in any test period, both p < Significant differences were observed at a risk rate of 0.05 to p <0.001.
On the other hand, in the habitual constipation, the pre-observation period, before and after the intake period of the placebo-added water, and before and after the rest period, the intake period before and after the intake period of LS-added water was 3.74 days and 3.47 days. A significant difference (p <0.05 to p <0.005) was recognized over the entire test period.
In the constipation-prone person, the 5.20 day of the first intake of LS-added water showed a significant difference (p <0.05 to p <0.005) in any test period with respect to all test periods. The late stage 4.77 days of ingestion of LS-added water showed a significant difference (p <005) only in the late rest period. On the other hand, in non-constipated persons, no significant difference was observed between each test period.
Next, in the comparison of the average number of defecations in one week for all subjects, the pre-observation period was 5.02 times, 5.18 times, 5.02 times, 5.12 times in order, compared with 4.90 times, and LS-containing water was added. The ingestion period was 6.17 times and 5.75 times, indicating an increase in the number of defecations in any test period, both of which showed a significant difference (p <0.05 to p <0.001). . In addition, in the habitual constipation, the intake period of the LS-added water increased to 4.16 times and 4.00 times, and there was a significant difference (p <0.05 to p <0. 005).
Moreover, among the constipation tendency person, the significant difference (p <0.05-p <0.005) was recognized in 6.00 times of the ingestion first stage of LS combination addition water in any test period. On the other hand, a significant difference (p <005) was observed only in the late period of intake of LS-containing added water compared to the pre- and post-rest periods. On the other hand, in non-constipated persons, no significant difference was observed between any test periods.
In comparison of the average defecation amount in one week for all subjects, when the size of one L-sized egg is taken as one unit, the previous observation period is 8.74 in the order of 7.85 and 9. 00, 8.54, and 9.16, and the initial period of intake of LS-containing added water was 10.73, indicating an increase in the amount of defecation in any test period, p <0.05 to p < A significant difference was observed with a risk factor of 0.001, but the 9.86 late-stage intakes of LS-added water were not significantly different from those before and after the rest period of placebo-added water.
In addition, in addictive constipation, the number of pre- and post-intake periods of LS-added water increased to 7.82 and 7.54, and was significantly significant for any test period other than the late stage of placebo-added water intake. A difference (p <0.05 to p <0.005) was observed. For those with a tendency to constipation, a significant difference (p <0.05 to p <0.005) was observed in the test period other than the first period of ingestion of placebo-added water, with 10.92 in the first period of ingestion of LS-added water. A significant difference (p <005) was observed only in the latter period of intake of added water compared to the previous observation period. On the other hand, in non-constipated persons, no significant difference was observed for each test period.

Effect on Fecal Properties The frequency of appearance of fecal properties in the total number of bowel movements during the entire test period was determined, and χ ² test was performed for the independence of the distribution. The results are shown in FIG.
Regarding the color of stool, the appearance rate of “yellowish brown” stool, which is considered to be good during the intake period of LS-added water, shows an increasing tendency compared to the intake period and rest period of placebo-added water, and “black” However, there was no statistically significant difference. As for the shape of the stool, the appearance rate of “Korokoro / Tick” in the previous observation period decreased from 32% to 19%, while “Banana / Semi-kneaded” increased by 10%, but there was a significant difference. There wasn't. In addition, there was no significant change in the appearance rate of “mud / water” stool between the test periods.
No significant difference was observed in the stool hardness, but “hard” tended to increase in the intake period of LS-containing added water than in the previous observation period. Regarding “stool odor”, “normal” in the previous observation period increased significantly (p <0.05) in the intake period and the pre-rest period of the LS-containing supplementary water, On the other hand, the occurrence rate of “ordinary” increased significantly (p <0.01), but “odor” remained unchanged.
Regarding the sensation after defecation, although there was a trend toward an increase in the appearance rate of “clean” in the intake period of LS-added water compared to other test periods, no statistically significant difference was observed. In addition, there was no significant change in the appearance rate of “normal” and “having a feeling of residual stool”.

Occurrence of Abdominal Symptoms As shown in FIG. 9, “shiburi abdomen” and “nausea” decreased in the intake period of LS-containing water compared to the previous observation period.
Although "wheezing", "abdominal pain" and "feeling fullness" were observed, there was no significant difference in the frequency of occurrence between the test periods.

Fecal bacterial flora The average number of bifidobacteria isolated from stool samples for each test period is shown in Table 3.

ビフィズス菌の菌数は前観察期で９．３３±０．５８（指数、ｃｆｕ／ｇ窺）、プラセボ付加水の摂取期が、９．２９±０．５６、休止期が９．２５±０．５８、ＬＳ配合付加水の摂取期が１０．０１±０．２７であった。この結果の有意差検定を行ったところ、ＬＳ配合付加水の摂取期の菌数は前観察期、休止期、プラセボ付加水の摂取期に対して何れにも有意（ｐ＜０．０５）な増加が認められた。

The number of bifidobacteria was 9.33 ± 0.58 (index, cfu / g 窺) in the previous observation period, 9.29 ± 0.56 during the placebo-added water intake period, and 9.25 ± 0 during the rest period .58, the intake period of the LS-added water was 10.01 ± 0.27. As a result of a significant difference test of the results, the number of bacteria in the intake period of LS-containing added water was significant (p <0.05) in any of the pre-observation period, rest period, and placebo-added water intake period. An increase was observed.

  On the other hand, Table 4 summarizes the occupancy and detection rate of bifidobacteria, lecithinase positive, coliforms relative to the total number of anaerobic bacteria in feces.

前観察期が１３．２０％、プラセボ付加水の摂取期が１０．１６％、休止期が１１．１８％に対して、ＬＳ配合付加水の摂取期が３８．０６％で有意（ｐ＜０．００５）な増加がみられた。
一方レシチナーゼ陽性、及び大腸菌群は、極少ない菌占有率を示し、有意差は認められなかった。また、図１０に各試験期間の全被験者のビフィズス菌の占有率とその平均を示した。
ＬＳ配合付加水の摂取期は他の試験期に比べ１２名全員の占有率は有意（ｐ＜０．００５）に上昇し、ＬＳ配合付加水を摂取することで糞便中のビフィズス菌の菌数及び占有率に改善効果がみられた。

The pre-observation period was 13.20%, the placebo-added water intake period was 10.16%, and the rest period was 11.18%, while the intake period of LS-added water was 38.06% and significant (p <0) (0.005) increase.
On the other hand, the lecithinase positive and the coliform group showed an extremely small occupancy rate, and no significant difference was observed. FIG. 10 shows the occupancy rate and average of bifidobacteria of all subjects during each test period.
The occupancy rate of all 12 individuals during the intake period of LS-containing supplementary water increased significantly (p <0.005) compared to other test periods, and the number of bifidobacteria in feces by ingesting LS-added supplementary water And the improvement effect was seen in the occupation rate.

Fecal pH, ammonia content and water content%
Table 5 shows the measurement results of pH, amount of ammonia, and water content% in feces.

被験者１２名の各試験期の各糞便サンプルのｐＨの比較において、プラセボ付加水の摂取期が前観察期に対しｐＨ上昇の有意差（ｐ＜０．００５）が認められた。
糞便中のアンモニア量は湿重量１ｇ当たり実験開始前が１．８３ｍｇ、プラセボ付加水の摂取期が１．８０ｍｇ、休止期が１．３９ｍｇとなり、これに対し、ＬＳ配合付加水の摂取期の１．３０ｍｇは減少傾向があるものの、有意差は認められなかった。
糞便中の水分量含有％は前観察期とプラセボ付加水の摂取期が各々７１．３７％及び７２．２８％、ＬＳ配合付加水の摂取期が７２．９８％と変化がなかった。

In the comparison of the pH of each stool sample of each of 12 subjects in each test period, a significant difference (p <0.005) in an increase in pH was observed in the placebo-added water intake period compared to the previous observation period.
The amount of ammonia in stool was 1.83 mg per gram of wet weight before the start of the experiment, 1.80 mg in the placebo-added water intake period, and 1.39 mg in the rest period. Although .30 mg tended to decrease, no significant difference was observed.
The water content% in the stool was unchanged in the previous observation period and the placebo-added water intake period of 71.37% and 72.28%, respectively, and the intake period of the LS-containing additional water was 72.98%.

Effect of increased intake of LS-containing supplementary water on defecation status and frequency of occurrence of abdominal symptoms For 20 subjects, “mud-like” for the total number of defecations during each test period when LS-added supplementary water increased intake test was conducted The excretion frequency of “stool” and “watery” stool is shown in Table 6.

ＬＳ配合付加水の常用量摂取Ａ期、２倍量摂取Ｃ期及び３倍量摂取Ｅ期の「泥状」便排泄頻度は各々１１．８％、6.6％及び７．５％と減少傾向があるものの、これらの摂取期間相互には有意差は見られず、用量依存性も認められなかった。また休止Ｂ期、休止Ｄ期においても１１．７％が８．１％に減少傾向にあった。

“Muddy” stool excretion frequency during the A-stage, 2-fold intake C-stage, and 3-fold intake E-period of LS-containing supplementary water is 11.8%, 6.6%, and 7.5%, respectively. Although there was no significant difference between these intake periods, there was no dose dependency. In addition, in the rest B phase and the rest D phase, 11.7% tended to decrease to 8.1%.

On the other hand, a significant p <0.05 increase was observed between the resting B phase and the resting D phase in the frequency of “watery” fecal excretion, but it was transient and did not affect the next week's study.
Table 7 shows the frequency of the number of people who complained of abdominal symptoms relative to the total number of people in each test period.

“Abdominal pain” decreased to 31.6% in the normal dose intake period A, 25.0% in the double intake period C, and 16.9% in the triple intake period E.
“Salmon” was scattered at an average of 12.5 to 17.5%, and “fullness” was changed within 29.6 to 43.1%, but no significant difference was observed between the tests.
“Rare” was found at 12.0-20.0%, and “bitter belly” varied within 3.2-7.7%, but no significant difference was observed between the tests. As for “nausea”, 1.3% was observed in the C phase ingestion of the double dose, but no other was observed.
Based on the above findings, 3.0 g / day as 1-fold dairy oligosaccharide 2a of LS-containing added water is 6.0 g / day as 2-fold dairy oligosaccharide 2a and 9-fold as 3-fold dairy oligosaccharide 2a. Even if the dose is increased to 0.0 g / day and ingested for 7 days each, no side effects due to ingestion of LS-added water were observed in the abdominal symptoms and mud / watery fecal excretion frequency during the triple intake period. It was.

Discussion It has been clarified that ingestion of dairy oligosaccharide 2a alone improves human bowel movements and stool properties, and intake of 1.0 to 2.0 g of dairy oligosaccharide 2a per day as a food material It is reported that bifidobacteria increase, and in addition, intake of 2.0 to 6.0 g of dairy oligosaccharide 2a per day significantly improves stool, decreases fecal spoilage substances and increases short-chain fatty acids. Yes. Even when processed into food form, defecation by ingesting cookies containing 2.0-5.0 g of dairy oligosaccharide 2a, lactic acid bacteria added water, powdered refreshed water, croissants, instant miso, confectionery, etc. It is proved that the situation is significantly improved and is used as food for specified health use.
Based on such findings, it is considered that an intake of 3.0 g of adult dairy oligosaccharide 2a per day is a reasonable normal dose, and 3 ml of dairy oligosaccharide 2a is obtained by ingesting 500 ml of drinking water per day. 3.0 g / 500 ml / book was produced as a PET bottle type LS-containing added water dairy oligosaccharide 2a so that 0.0 g could be ingested.
Furthermore, placebo-added water from which dairy oligosaccharide 2a was removed was prepared as a control. Since this product is in the same form as the plastic bottle containers for added water on the market, it seems that the daily requirement of 500 ml / book can be clearly appealed. In addition to being able to be taken regardless of meals, it is not necessary to cook and is convenient to carry, so it can be assumed that it is likely to fall into excessive intake in summer.

In this study, we observed the effects of ingestion of LS-containing water with 500 ml dairy oligosaccharide 2a per day on 3.0 g for 2 weeks on the days of stool, number of stools, amount of stool, stool properties and abdominal symptoms. In addition, for female students of a different group, the daily dose of 500 ml is doubled to 1,000 ml, the dairy oligosaccharide 2a is 6.0 g, and the daily triple amount is 1,500 ml. The influence on the frequency of mud / watery fecal excretion and the incidence of abdominal symptoms when the amount was increased to 9.0 g as oligosaccharide 2a and ingested for 7 days each was observed.
The evaluation of the influence on the defecation status by taking the normal dose of LS-added water was obtained from the significant difference test of the number of defecation days, the number of defecations, and the amount of defecation obtained in each test period.
As a result, the effects of significantly increasing the number of days of stool, the number of stool and the amount of stool in the other test periods were observed in the intake period before and after the intake period of the LS-added water.

According to reports by Iino et al., Iwai et al. And Kitao et al., It was reported that the effect of dairy oligosaccharide 2a on the defecation status was more pronounced in subjects with a strong tendency to constipation. As a result of stratified analysis divided into constipation, constipation prone and non-constipation, the intake of LS compounded water is the strongest in addictive constipation with less than 3 defecation per week. Admitted. In addition, constipation-prone stool frequency was improved 4 to 7 times a week in the first half of the intake of LS-added water and continued until the second half of the following week.
Therefore, a significant improvement effect was observed for both constipation and constipation prone. However, no significant difference was observed when the number of defecations of non-constipated persons was 8 or more per week.

Next, the intake of LS-added water showed significant improvement in stool properties in some of the observation items of "stool color", "stool shape", and "stool hardness", but "stool odor" As for, in each test period compared to the previous observation period, “not interested” decreased and “normal” significantly increased to p <0.05. On the other hand, since no change was observed for “Kusai”, it seemed unlikely that abnormal intestinal fermentation was caused by ingestion of LS-added water.
The average number of bifidobacteria increased significantly to p <0.005 with respect to the whole test period by ingesting LS-added water for 2 weeks. In addition, the occupancy rate of bifidobacteria during the intake period of LS-containing supplementary water was also significantly increased to p <0.005 among all 12 subjects over the entire test period, and total anaerobic bacteria due to the intake of LS-added supplementary water for two weeks. The occupancy improvement effect of bifidobacteria was observed.
In this test, the fecal pH value was higher in each test period than in the entire observation period. It was considered that this was influenced by the added water because the pH of both the test drink and the placebo drink was 7.2, which was higher than the acid side. However, since the intake period of LS-added water has a lower pH than the intake period of placebo-added water, it was predicted that intestinal production of organic acids and the like was performed. On the other hand, in the case of the stool water content%, it was considered that it would be difficult to make a difference between each test by continuously taking 500 ml of water every day.

By the way, the test additional water of this time can be ingested irrespective of the food as compared with other food forms, and there is also a drawback that it is easy to ingest the necessary amount and at the same time easily fall into excessive intake. The maximum amount of ineffectiveness against diarrhea when dairy oligosaccharide 2a as a raw material is ingested as a simple substance is 0.6 g per 1 kg body weight. It has been reported that ingestion does not induce diarrhea. According to Ohkusa et al., The result of a human test in which a daily amount of 6.0 g of dairy oligosaccharide 2a was ingested for 8 weeks as a raw material and the safety of long-term ingestion of dairy oligosaccharide 2a was evaluated was 8 Flatulence and diarrhea were observed in one of the names, but none of these side effects were reported to be transient and persistent.
In the overdose test of this test, 20 subjects were increased from one daily dose, and a test was conducted in which a doubled dose and a tripled dose that was one third of the maximum no-effect level were taken. The intake of LS-containing supplementary water is limited to the daily intake, but the intake time and the amount to be taken at one time are not specified. If they set their own intake schedule and couldn't drink it, it was used for coffee and cooking, and it was said that they made efforts to complete the prescribed intake.

The frequency of mucous fecal excretion after ingesting 3 times normal dose of LS-added water was 7.5% (10 out of 135 stools), which was less than in the normal dose ingestion period. In addition, there was no example in which mud stool was continuously observed, and all were transient on the following day. In addition, the frequency of “watery” fecal excretion was significantly (p <0.05) only during rest periods B and D, but no significant difference was observed compared to other test periods.
In the frequency of occurrence of abdominal symptoms, no significant difference was observed between any symptoms in the analysis results by χ ² test. Regarding complaints of “abdominal pain”, which is a relatively severe abdominal symptom, it decreased to 31.6% in the normal dose intake period, 25.0% in the double dose intake period, and 16.9% in the triple dose intake. A trend was observed. However, 90% of the “abdominal pain” persistence during the triple intake period returned to normal within 1 day, and only 2 cases persisted for more than 3 days. Since it was not observed, the frequency of occurrence of “abdominal pain” in the triple dose intake period was transient, and it was considered that it was not a continuous symptom.

When using concentrated type functional additional water, dilute with tap water or natural water 1A. In that case, if natural water 1A is increased, it will become tastefully soft.
One of the features of the functional added water of the present invention is that the mineral components contained in the desalted water 1B and the desalted deep water 1C are substantially the same as the mineral components constituting the human body, and The balance is also close.

As the deep ocean water C, cold water specific to the Sea of Japan is suitable. The reason for this is that the cold water of the Sea of Japan occupies about 65% of the volume of Toyama Bay. As described in JP 2000-290168, JP 2000-290161, etc., it is slightly different from the deep ocean water in Kochi Prefecture. Unlike the surface water, there is almost no change in water temperature at a low temperature of 2 ° C. or less throughout the year, the salt content is 34.0-34.1 psu, and the nutrients are significantly more abundant than the surface water. And very few bacteria.
The deep ocean water C has many characteristics such as low generation of microorganisms at low temperature and high cleanliness.

  The sorting accommodation form is not limited to the embodiment, and for example, a part of the first container body 16 is provided with a concave step part and an engaging part 19, and the second container 25 is formed in a shape corresponding to the concave step part. If the engaged portion 29 that engages with the engaging portion 19 is provided, the second container 25 is engaged with the recessed step portion, and the engaged portion 29 is engaged with the engaging portion 19, the first is used until it is used. The separation loss between the container 15 and the second container 25 can be prevented.

It is a block diagram which shows the example of a combination of the component in the functional addition water of this invention. It is a block diagram which shows a multistage electrodialysis method. It is a block diagram which shows a dehydration separation means. It is a mineral component diagram of deep ocean water and fresh water, mineral concentrated water, and concentrated salt water separated therefrom. It is a front view which shows 1st embodiment of the separation accommodation state by a 1st container and a 2nd container. It is the 1st container top view (I) and the 2nd container sectional view (B) showing the second embodiment of a separation accommodation state. It is a front view of the lid which shows a 3rd embodiment of a separation accommodation state. It is sectional drawing (b) before a fracture | rupture which shows 1st embodiment of the isolation | separation accommodation state using an inside plug body, sectional drawing (b) after fracture | rupture, and top view (c) before fracture | rupture. It is sectional drawing (a) before a fracture | rupture which shows 2nd embodiment of the isolation | separation accommodation state using an inside plug body, and sectional drawing (b) after a fracture | rupture. It is process drawing which shows the manufacturing method of functional addition water by this invention. It is a test schedule wool figure. It is a schedule figure in tests 1, 2, and 3. It is a graph which shows the frequency of occurrence of fecal properties. It is a graph which shows the frequency of occurrence of abdominal symptoms. It is a graph which shows the change of a bifidobacterium occupation rate.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Mineral-containing water 1A Natural water, 1a Mineral water B Sea water, 1B Desalted water C Deep sea water, 1C Desalted deep water 2 Indigestible saccharide 2A Water-soluble starch, 2a Oligosaccharide (milk oligosaccharide)
2B Low molecular weight dextrin 3 Primary mixture, 13 Secondary mixture 5, 15, 25 Container 6, 16, 26 Container body, 6a, 16a, 26a Body trunk, 6b Body mouth 7, 17, 27 Lid, 7a Lid Plate, 7b Circumferential wall, 7c Pressing part 8 Middle plug body, 8a Housing part, 8b Ridge part, 8c Breaking part 37 Auxiliary wall, 47 Needle 14 Connecting part, 24 Connected part 19 Fitting part, 29 Fitted part 10 Multistage Electrodialysis 11 First treatment device, M1 ion exchange membrane 12 Second treatment device, M2 ion exchange membrane 20 Dehydration separation means T, 3T, 4T Fresh water U, 3U, 4U First concentrated salt water V, 3V, 4V Mineral concentration Water W, 3W, 4W Second concentrated salt water X, 3X, 4X Negative Y, 3Y, 4Y Salt

Claims (9)

  Functional addition water characterized by comprising an indigestible saccharide (2) and mineral-containing water (1) having a hardness of 10 or more.   Mineral-containing water (1) is separated from natural water (1A) pumped from the ground, demineralized water (1B) obtained by separating from seawater (B), and deep ocean water (C) 200 meters or less below sea level. The functional additional water according to claim 1, wherein the functional additional water is one selected from the desalted deep water (1C) obtained in the above.   Mineral-containing water (1) is separated from natural water (1A) pumped from the ground, demineralized water (1B) obtained by separation from seawater (B), and deep ocean water (C) 200 meters or less below sea level. The functional additional water according to claim 1, wherein the functional additional water is at least two selected from the desalted deep water (1C) obtained in the above.   Desalinated water (1B) and desalted deep water (1C) were separated by fresh water (T) and mineral concentrate (V) separated by multistage electrodialysis (10) and by multistage electrodialysis (10). The functional added water according to claim 2 or 3, wherein the second concentrated salt water (W) is at least one selected from bittern (X) obtained through dehydration separation means (20). .   The functional added water according to claim 1, 2, 3 or 4, wherein the hardly digestible saccharide (2) is a water-soluble starch (2A) having 3 or more monosaccharides bound thereto.   The water-soluble starch (2A) is an oligosaccharide (2a), and this oligosaccharide (2a) is a direct use type in which it is mixed in a range of 0.1 to 10%. The functional additional water according to claim 5, wherein   The water-soluble starch (2A) is an oligosaccharide (2a) and is a concentrated type in which the oligosaccharide (6) is mixed in a range of 2 to 70%, and is used after being diluted. Functional addition water described.   The mineral-containing water (1) and the indigestible saccharide (2) are stored separately, and the mineral-containing water (1) and the indigestible saccharide (2) are mixed and used at the time of use. Functional added water.   A primary mixture of the non-digestible carbohydrate (2) and a part of the mineral-containing water (1), the secondary mixture of the primary mixture (3) and the remaining mineral-containing water (1), and then the secondary mixture A method for producing functional added water, wherein the container (5) is hermetically filled in (13) through primary filtration, sterilization, and secondary filtration.

Images