JP2006126152A - 新規化学発光試薬 - Google Patents
新規化学発光試薬 Download PDFInfo
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- JP2006126152A JP2006126152A JP2004338145A JP2004338145A JP2006126152A JP 2006126152 A JP2006126152 A JP 2006126152A JP 2004338145 A JP2004338145 A JP 2004338145A JP 2004338145 A JP2004338145 A JP 2004338145A JP 2006126152 A JP2006126152 A JP 2006126152A
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- imidazo
- pyrazin
- sulforhodamine
- rhodamine
- derivative
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- 0 *C1=NC2=CNC(c(cc3)ccc3O)=CN2C1O Chemical compound *C1=NC2=CNC(c(cc3)ccc3O)=CN2C1O 0.000 description 2
- JIYRMOMGONACEP-UHFFFAOYSA-N CC1=NC2=CNC(c(cc3)ccc3OCCCC(NC(Nc(cc3C(O)=O)ccc3C(C(C(OC3C4)=C5)=CCC5O)=C3C=CC4=O)=S)=O)=CN2C1=O Chemical compound CC1=NC2=CNC(c(cc3)ccc3OCCCC(NC(Nc(cc3C(O)=O)ccc3C(C(C(OC3C4)=C5)=CCC5O)=C3C=CC4=O)=S)=O)=CN2C1=O JIYRMOMGONACEP-UHFFFAOYSA-N 0.000 description 1
- JYQHFNUMLIRXCQ-UHFFFAOYSA-N CC1=NC2=CNC(c3ccccc3)=CN2C1=O Chemical compound CC1=NC2=CNC(c3ccccc3)=CN2C1=O JYQHFNUMLIRXCQ-UHFFFAOYSA-N 0.000 description 1
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Abstract
【解決手段】 スーパーオキシドアニオンと反応することにより発光するイミダゾ[1,2−a]ピラジン−3−オン類などと蛍光色素とをスペーサーを介して結合させた。そして,蛍光色素は,その励起スペクトルがイミダゾ[1,2−a]ピラジン−3−オン類の発光スペクトルと重なる色素としてローダミン類を選択し,スムーズな発光エネルギー共鳴移動を行なわせることで上記課題を解決した。
【選択図】 図1
Description
青紫色粉末
1H NMR(500MHz,23℃,DMSO−d6):1.7−2.1(16H,m),2,4−3.8(40H,m),6.28(2H,s),6.50(2H,s),6.87(1H,d,J=7.9Hz),7.36(1H,d,J=7.9Hz),7.78(1H,dd,J=1.8,7.9Hz),7.88(1H,d,J=1.8Hz),7.90(1H,dd,J=1.8,7.9Hz),8.40(1H,d,J=1.8Hz)ppm
IR(KBr):3424,2941,1598,1497,1800,1198,1101,1035cm−1
UV−vis(MeOH):268(ε24500),297(ε15300),369(ε5540),424(ε2100),584(ε83800)nm
紅青紫色粉末
1H NMR(500MHz,23℃,CD3OD−DMSO−d6=10:1)1.8−3.6(64H,m),2.14(3H,s),2.43(3H,s),4.00(4H,m),6.54(2H,s),6.58(2H,s),6.94(2H,d,J=8.6Hz),7.01(2H,d,J=8.6Hz),7.32(1H,d,J=7.9Hz),7.43(1H,d,J=7.9Hz)17.51(2H,d,J=8.6Hz),7.56(1H,br.s),7.58(2H,d,J=8.5Hz),7.61(1H,br.s),7.72(1H,br.s),7.90(1H,br.s),8.03(1H,dd,J=1.8,7.9Hz),8.18(1H,d,J=7.9Hz),8.53(1H,s),8.61(1H,dd,J=1.8,7.9Hz)ppm
IR(KBr):3423,2942,1596,1496,1298,1198,1101,1035cm−1
UV−vis(MeOH):268(ε47700),369(ε11800),428(ε10000),584(ε99100)nm
青紫色粉末
1H NMR(500MHz,23℃,1.0%TFA/D2O−DMSO−d6=10:1):1.7−2.0(m),2.4−3.0(m),3.3−3.8(m),6.44(2H,s),7.05(2H,d,J=7.9Hz),7.36(1H,d,J=7.9Hz),7.72(2H,d,J=7.9Hz),7.90(1H,s),8.01(1H,d,J=7.9Hz),8.26(1H,s),8.37(1H,s)ppm.
IR(KBr):3423 2939,1597,1497,1299,1198,1101,1035cm−1.
UV−vis(MeOH):268(ε39400),370(ε9320),433(ε8090),587(ε84800)nm.
Claims (4)
- Dがイミダゾ[1,2−a]ピラジン−3−オン誘導体,Aが1以上の遊離スルホン酸あるいはスルホン酸塩を有するローダミン123,ローダミンB,ローダミン6G,ローダミン19,ローダミン110,ローダミン575,あるいはスルホローダミン101から選択され,Sがアルキレン,アミド結合を有するアルキレン,アザアルキレン,アミド結合を有するアザアルキレン,ポリアザアルキレン,アミド結合を有するポリアザアルキレン,オキサアルキレン,アミド結合を有するオキサアルキレン,ポリオキサアルキレン,あるいはアミド結合を有するポリオキサアルキレンから選択される請求項1記載の新規化学発光試薬。
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JP2004338145A JP4453528B2 (ja) | 2004-10-26 | 2004-10-26 | 新規化学発光試薬 |
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JP2004338145A JP4453528B2 (ja) | 2004-10-26 | 2004-10-26 | 新規化学発光試薬 |
Publications (2)
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JP2006126152A true JP2006126152A (ja) | 2006-05-18 |
JP4453528B2 JP4453528B2 (ja) | 2010-04-21 |
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JP2004338145A Expired - Fee Related JP4453528B2 (ja) | 2004-10-26 | 2004-10-26 | 新規化学発光試薬 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013544242A (ja) * | 2010-11-02 | 2013-12-12 | プロメガ コーポレイション | セレンテラジン誘導体およびその使用方法 |
CN104165877A (zh) * | 2014-08-31 | 2014-11-26 | 桂林理工大学 | 一种检测超氧阴离子自由基含量的方法 |
CN112268895A (zh) * | 2020-09-04 | 2021-01-26 | 中国能源建设集团安徽省电力设计院有限公司 | 采用化学发光试剂检测水中微量超氧根离子自由基浓度的装置及方法 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4982735B2 (ja) * | 2005-10-06 | 2012-07-25 | 国立大学法人三重大学 | 化学発光物質 |
CN107884349B (zh) * | 2017-10-13 | 2020-12-15 | 昆明理工大学 | 一种微生物体内超氧阴离子自由基的测定方法 |
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2004
- 2004-10-26 JP JP2004338145A patent/JP4453528B2/ja not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013544242A (ja) * | 2010-11-02 | 2013-12-12 | プロメガ コーポレイション | セレンテラジン誘導体およびその使用方法 |
CN104165877A (zh) * | 2014-08-31 | 2014-11-26 | 桂林理工大学 | 一种检测超氧阴离子自由基含量的方法 |
CN112268895A (zh) * | 2020-09-04 | 2021-01-26 | 中国能源建设集团安徽省电力设计院有限公司 | 采用化学发光试剂检测水中微量超氧根离子自由基浓度的装置及方法 |
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