JP2006045115A - Compounded aqueous gel, method for producing the same, gelled preparation containing compounded gel, skin preparation for external use, and cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a compounded aqueous gel formed by interacting a specific ionic amphiphilic polymer with an amphoteric ionic polymer and having thixotropic property, a gelled preparation containing the compounded aqueous gel, a skin preparation for external use and cosmetic, and also the compounded aqueous gel, without a fear of getting the breakage of the gel structure caused by a cationic component, pH buffer, salt such as sodium chloride and powdery material such as titanium oxide, iron oxide, etc., safe for the skin, even on adjusting pH in the vicinity of 3-5.5, capable of maintaining a high viscosity and the thixotropic property, a gelled preparation containing the compounded aqueous gel, the skin preparation for external use and cosmetic. <P>SOLUTION: This method for obtaining the compounded aqueous gel is characterized by making the ionic amphiphilic polymer interact with amphoteric ionic polymer for compounding to obtain the compounded aqueous gel. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention contains a thixotropic composite aqueous gel formed by interacting a zwitterionic polymer with a specific ionic amphiphilic polymer, a method for producing the same, and the composite aqueous gel The present invention relates to a gelled preparation, an external preparation for skin, and a cosmetic.

Gels containing water as a continuous phase, that is, aqueous gels, have been used in cosmetics in recent years because they are preferred for use in summer and for a refreshing feeling. As such an aqueous gelling agent, a carboxyvinyl polymer that is mainly an anionic polymer has been conventionally used because of its high viscosity and excellent thixotropic properties. As cosmetics and skin external preparations to be included, patent applications such as Patent Document 1 and Patent Document 2 below have been filed.
JP 2004-91360 A JP 2000-239147 A

  However, since the carboxyvinyl polymer is negatively charged, for example, if a cationic active ingredient is included in cosmetics or external preparations for skin, a precipitate is formed by an ion complex, and the effect of the drug or active ingredient is drastically reduced. There is a problem. Further, since it is gelled by neutralization with an alkali such as sodium hydroxide, it is difficult to adjust the pH to around 3 to 5.5 which is safe and useful for the skin. In addition, chemical peeling agents such as glycolic acid and salicylic acid are known to exert their effects effectively on the acidic side of pH 3 to 4, but this also makes it difficult to adjust in the vicinity of pH 3 to 5.5. That is not preferable. Furthermore, there is a problem that gel structure breakage easily occurs due to a salt such as sodium chloride or a pH buffering agent, or a powder such as titanium oxide or iron oxide.

On the other hand, cellulose derivatives such as hydroxypropylcellulose are also used as gelling agents for nonionic polymers, and as cosmetics and skin external preparations containing such hydroxypropylcellulose, for example, Patent Document 2 and Patent Documents listed below can be used. Patent applications such as No. 3 have been filed.
JP 2000-204016 A

  However, such hydroxypropyl cellulose has a problem that the viscosity and thixotropy of the gel are low.

  The present invention has been made to solve such a conventional problem, and there is a risk that gel structure destruction may occur due to a cationic component, a pH buffer, a salt such as sodium chloride, or a powder such as titanium oxide or iron oxide. There is also a composite aqueous gel that is safe to the skin and can maintain high viscosity and thixotropic properties even when adjusted to around pH 3 to 5.5, and a gelled preparation containing the composite aqueous gel, and for external use on the skin It is an object to provide agents and cosmetics.

  The inventors of the present invention have intensively studied to solve such a problem. As a result, a high viscosity (viscosity: 10,000) is obtained by allowing a specific ionic amphiphilic polymer to interact with and complex with a specific ionic amphiphilic polymer. A gel having a thixotropy of ˜2,000,000 cps) was obtained, and it was found that the conventional problems were improved, and the present invention was completed.

  That is, the invention according to claim 1 relating to the composite aqueous gel is characterized by containing an ionic amphiphilic polymer and an amphoteric polymer. The invention according to claim 2 is the composite aqueous gel according to claim 1, wherein the ionic amphiphilic polymer is partially introduced with 0.1 to 50.0% of a hydrophobic group having 8 to 22 carbon atoms. It is characterized by that. Furthermore, the invention according to claim 3 is the composite aqueous gel according to claim 1 or 2, wherein the ionic amphiphilic polymer has an amino group.

  The invention according to claim 4 is the composite aqueous gel according to any one of claims 1 to 3, wherein the ionic amphiphilic polymer is chitin, chitosan, a chitin derivative, or a chitosan derivative. And Furthermore, the invention according to claim 5 is the composite gel according to claim 4, wherein the chitin, chitosan, chitin derivative, or chitosan derivative contains 0.1 to 50.0% of a hydrophobic group having 8 to 22 carbon atoms. It is what was introduced. Furthermore, the invention according to claim 6 is the composite aqueous gel according to any one of claims 1 to 5, wherein the weight ratio of the ionic amphiphilic polymer to the amphoteric polymer is from 1: 0.1 to 0.1. It is in the range of 1: 10.0.

  Furthermore, the invention according to claim 7 is characterized in that in the composite aqueous gel according to any one of claims 1 to 6, the zeta potential of the zwitterionic polymer is -20 mV to +20 mV. The invention according to claim 8 is the composite aqueous gel according to any one of claims 1 to 7, wherein the amphoteric polymer has a phospholipid structure. The invention according to claim 9 is the composite aqueous gel according to any one of claims 1 to 7, wherein the zwitterionic polymer has a phospholipid structure and a hydrophobic group. To do.

  Furthermore, the invention according to claim 10 is the composite aqueous gel according to any one of claims 1 to 9, wherein the pH of the ionic amphiphilic polymer and the amphoteric polymer in a composite state is 3. The zeta potential at 5 to 5.5 is 0 mV to +80 mV. Furthermore, the invention according to claim 11 is the composite aqueous gel according to any one of claims 1 to 10, wherein a polyhydric alcohol is contained in addition to the ionic amphiphilic polymer and the amphoteric polymer. It is characterized by being.

  Furthermore, the invention according to claim 12 relating to the method for producing a composite aqueous gel comprises mixing an ionic amphiphilic polymer and an amphoteric polymer, A complex aqueous gel is produced by complexing molecules. The invention according to claim 13 is the method for producing a composite aqueous gel according to claim 12, wherein the weight ratio of the ionic amphiphilic polymer to the amphoteric polymer is from 1: 0.1 to 1: It is characterized by being produced by combining an ionic amphiphilic polymer with an amphoteric polymer so as to be within the range of 10.0.

  Furthermore, the invention according to claim 14 is an invention relating to a gelled preparation comprising the composite aqueous gel according to any one of claims 1 to 11, and the invention according to claim 15 is any one of claims 1 to 11. It is invention which concerns on the skin external preparation characterized by including the composite water gel as described above. Furthermore, the invention described in claim 16 is characterized in that the external preparation for skin described in claim 15 contains a cationic component in addition to the composite aqueous gel.

  Furthermore, an invention according to claim 17 is an invention relating to a cosmetic comprising the composite aqueous gel according to any one of claims 1 to 11, and the invention according to claim 18 is an invention according to claim The cosmetic according to 17, wherein the cosmetic contains a cationic component in addition to the composite aqueous gel.

  By using the composite aqueous gel of the present invention, it is stable even in the presence of cationic active ingredients, chemical peeling agents such as glycolic acid, salts such as pH buffer, and powders and pigments, which were difficult in the past.・ Highly effective and safe and useful for skin, under the control of pH around 3 to 5.5, gel-like skin preparations and cosmetics with excellent viscosity and thixotropy, gel-like emulsification preparations and emulsification makeup There is an effect that it can be provided as a pharmaceutical preparation.

  The composite aqueous gel of the present invention contains an ionic amphiphilic polymer and an amphoteric polymer as described above, and as the ionic amphiphilic polymer, A polyamine having an amino group and 0.1 to 50.0% partially introduced of a hydrophobic group having 8 to 22 carbon atoms is used. Specific examples of polyamines introduced with hydrophobic groups include silk proteins such as gelatin, polylysine, collagen and sericin, vegetable proteins such as soybean protein, derivatives of these proteins, synthetic polymers such as polyvinylamine, polyethyleneimine and polyamidoamine. And chitosan and chitin polysaccharides and derivatives thereof. The average molecular weight is not particularly limited, but is preferably about 10,000 to 5000000.

  Chitosan or chitin, or chitin or chitosan derivatives include, for example, carboxymethyl chitin, carboxymethyl chitosan, succinylated chitosan, partially hydrolyzed chitin, sulfated chitin, sulfated chitosan, phosphorylated chitosan, phosphorylated chitin, dicarboxymethyl Chitosan, carboxybutyl chitosan, glycol chitosan, succinylated carboxymethyl chitosan, succinylated chitin, hydroxyalkyl chitosan such as hydroxypropyl chitosan, quaternary chloride chitosan, pyrrolidone carboxylate of chitin and chitosan, glycolate, glutamate, ascorbine Examples thereof include chitin such as acid salt and lactate, and salts of chitosan.

  The average molecular weight of chitosan or chitin, or chitin and chitosan derivatives is not particularly limited, but it is preferable to use one having an average molecular weight of about 10,000 to 5,000,000 from the viewpoint of complexing an amphoteric polymer. The average molecular weight in this case is, for example, GPC-HPLC coupled with a data module GPC cartridge [gel filtration chromatography column: TSK-gel-G3000WXL + TSK-gel-G2500PWXL manufactured by Tosoh Corporation, solvent: 0.4 M acetic acid-acetic acid Na buffer (pH = 4.8)] Measured by revealing molecular weight distribution by analysis. As the molecular weight standard, chitosan oligosaccharide (molecular weight: 413 to 1006), dextran sulfate (molecular weight: 5000, 8000) and pullulan molecular weight standard are used. The average molecular weight can also be obtained from a viscosity measurement method or the like.

  Moreover, as chitin or chitosan, or a derivative of chitin or chitosan, it is preferable to use one having an amino group content of 10 to 100%. Chitosan is a highly deacetylated product of chitin, which is a natural polysaccharide, and it is preferable to synthesize derivatives based on deacetylated chitin that exhibits a degree of deacetylation of 50 to 100%.

  Furthermore, from the viewpoint of forming a composite aqueous gel having excellent viscosity, it is preferable to introduce 0.1 to 50.0% of a hydrophobic group having 8 to 22 carbon atoms into the amino group or hydroxyl group of these derivatives. The type of hydrophobic group is not particularly limited, but a fatty acid group is desirable. Specifically, partially oleylated chitosan pyrrolidone carboxylate, partially myristoylated chitosan pyrrolidone carboxylate (product name: PM-chitosan: Pierce Co., Ltd.), partially myristoylated chitin lactate, partially myristoylated quaternized chitosan glyco -Lurate, partially lauroylated chitosan pyrrolidone carboxylate, partially myristoylated succinyl chitosan, partially myristoylated carboxymethyl chitosan, partially lauroylated carboxymethyl chitosan, partially acetyl myristoylated chitosan pyrrolidone carboxylate, etc. can be used .

  Here, “partial introduction” indicates how much fatty acid groups such as acyl groups are introduced per residue of the constituent monosaccharide. “Partial introduction of 0.1 to 50.0% of fatty acid groups” For example, in the case of chitosan, 1 to 500 fatty acid groups are introduced into 1000 residues of hexosamine, which is a constituent monosaccharide. Thus, chitin and chitosan derivatives into which fatty acid groups such as acyl groups having 8 to 20 carbon atoms are partially introduced exhibit amphipathic properties.

  Furthermore, in the present invention, the concept of “partial introduction” is also used for ionic amphiphilic polymers other than chitin, chitosan, or derivatives thereof. “Partial introduction” in this case indicates how much a hydrophobic group is introduced per residue of a monomer or the like, and “0.1 to 50.0% of a hydrophobic group was partially introduced”. For example, in the case of proteins such as gelatin, polylysine, collagen, sericin, soybean protein, etc., it means that 1 to 500 hydrophobic groups are introduced into 1000 amino acid residues constituting these proteins. . In the case of synthetic polymers such as polyvinylamine, polyethyleneimine, and polyamidoamine, it means that 1 to 500 hydrophobic groups are introduced into 1000 residues of monomers constituting these synthetic polymers.

  It is essential for the amphoteric polymer used in the present invention to contain both an anionic group and a cationic group in the molecule, and the type is not particularly limited. The molecular weight of the amphoteric polymer is not particularly limited, but an average molecular weight of about 50,000 to 2,000,000 is desirable.

  The zeta potential that serves as an indicator of the charge of the zwitterionic polymer is preferably −20 mV to +20 mV. The zeta potential indicates the surface potential that governs the electrostatic repulsion effect by the interfacial electric double layer. When the zeta potential of the zwitterionic polymer is -20 mV to +20 mV, it is combined with the ionic amphiphilic polymer. It is easy to cause. More specifically, when the ionic amphiphilic polymer is a chitosan derivative, the zeta potential is about +50 mV to +120 mV, but it is combined with a zwitterionic polymer having a zeta potential of −20 mV to +20 mV. As a result, a complex aqueous gel having a zeta potential of 0 mV to +80 mV at pH 3.5 to 5.5 is obtained, and the complexation is preferably caused. The amphoteric polymer preferably contains a hydrophobic site having 2 to 20 carbon atoms. Furthermore, it is desirable to have a phospholipid-like structure in the molecule from the viewpoints of biocompatibility and gel characteristics.

  Examples of the phospholipid-like structure include a component containing polymethacryloyloxyethyl phosphorylcholine and a hydrophobic group-containing monomer having 2 to 20 carbon atoms. Specific examples include polymethacryloyloxyethyl phosphorylcholine / butyl methacrylate copolymer (product name: lipid) containing monomers of polymethacryloyloxyethyl phosphorylcholine and butyl methacrylate.

  Synthesized from ionic amphiphilic polymer and zwitterionic polymer, the amino group part of ionic amphiphilic polymer and zwitterionic polymer are combined, and ionic amphiphilic polymer and zwitterion By imparting a hydrophobic bond with the hydrophobic polymer, a composite aqueous gel excellent in viscosity and thixotropy can be obtained. In the case of producing a composite aqueous gel, while stirring an aqueous solution of an ionic amphiphilic polymer uniformly, 0.1 to 10.0 times the amount of the amphoteric polymer is gradually added, and a homomixer is added. By carrying out uniform treatment with a stirrer such as (5000 rpm, 1 minute treatment), a gel excellent in viscosity and thixotropy can be obtained. The composite can be prepared even at room temperature, and has an advantage of excellent economic efficiency.

  The mechanism by which a zwitterionic polymer is combined with an ionic amphiphilic polymer having an amino group to form a gel is presumed as follows. That is, the anionic sites of the amphoteric polymer are selectively weakly bound to the amino groups of the ionic amphiphilic polymer formed from polyamines, while A hydrophobic bond with the hydrophobic group of the molecule is also given, and as a result, the polymer chains form a three-dimensional network structure / gel network structure uniformly. In the composite aqueous gel of the present invention, preferably, the anion sites in the zwitterion of the zwitterionic polymer are selectively weakly bonded to the amino group or charged amino group of the ionic amphiphilic polymer formed from polyamine. While acting, the polymer chains form a uniform three-dimensional network structure / gel network structure, and change into a gel excellent in viscosity and thixotropy.

  The composite gel of the present invention exhibits high viscosity and thixotropy even under the coexistence of a salt such as a cationic active ingredient and a pH buffer, and in the vicinity of pH 3.5 to 5.5, which is safe and useful for skin and hair. Therefore, it can be applied to skin external preparations and cosmetics with better effectiveness, safety, stability, and usability. Since there is no reduction in the efficacy of the cationic active ingredient and the safety is further increased, it can also be applied to cosmetics for sensitive skin and DDS (drug delivery system) formulations. As described above, the present invention is also intended for skin external preparations, cosmetics, and other gelled preparations containing the above complexed aqueous gel. In addition to the complexed aqueous gel, the cationic component as described above is used. Can coexist. Cationic components include cationic antibacterial agents such as benzalkonium chloride, cetylpyridinium chloride, and phthalhexidine gluconate, hydrochloride active ingredients and drugs such as berberine chloride and bunazodine hydrochloride, and alkylammonium such as stearyltrimethylammonium chloride. Salts, amidoamine compounds such as ethylaminoethyl stearate, aromatic ammonium salts such as benzethonium chloride, amino acid compounds such as N-α-long chain acyl basic amino acid alkyl esters and salts thereof, and the like can be used.

  As described above, the composite aqueous gel of the present invention has a high viscosity by being combined by the interaction of an ionic amphiphilic polymer and an amphoteric polymer. It is not limited. However, in order to obtain a gel having excellent properties such as thixotropy, the viscosity is preferably 10,000 to 2,000,000 cps.

  The composition ratio (weight ratio) of the ionic amphiphilic polymer [component (a)] and the amphoteric polymer [component (b)] constituting the composite aqueous gel of the present invention is not particularly limited. However, (a) :( b) = 1: 0.01 to 1: 100 is preferable, and (a) :( b) = 1: 0.1 to 1: 10.0 is more preferable. When the weight ratio of the component (b) to the component (a) is less than 0.1, gel formation is insufficient and the functionality cannot be sufficiently exhibited. On the other hand, when the weight ratio of the component (b) to the component (a) exceeds 10, the gel strength is reduced beyond the maximum point of gel formation, and the functionality of the composite gel becomes insufficient.

  Furthermore, it is desirable that the composite aqueous gel of the present invention coexists with a polyhydric alcohol in order to improve the aging stability of gel properties. Examples of the polyhydric alcohol include 1,3-butylene glycol, dipropylene glycol, glycerin, diglycerin, pentadiol, polyethylene glycol, sorbitol, mannitol and the like.

  Since the composite aqueous gel of the present invention has increased functionality derived from amino groups, the ion complex formation is low, and the amino groups are protected while improving the efficacy function and its sustainability. The composite aqueous gel of the present invention is highly stable and hardly shows temperature change or gel strength and physical properties over time.

  The present invention can be widely applied to pharmaceuticals to be applied to the skin, skin and hair, quasi-drugs to be applied to the oral cavity, cosmetics, and the like. It can be applied to a wide range of preparations such as a viscous sol system, essence system, liquid system, water-oil two-layer system, and powder-containing system. Furthermore, it can be provided in the form of preparations such as lotions, emulsions, packs, emulsified lotions and creams, regardless of the form of the external preparation for skin and cosmetics containing the composite aqueous gel of the present invention. For example, it can be provided as a preparation such as a creamy skin care agent, an emulsion makeup base, an emulsified mascara or a foundation.

  In addition, components generally used in external preparations for skin and cosmetics can be widely blended as long as the effects and functionality of the composite aqueous gel of the present invention are not impaired. For example, higher alcohols such as cetanol and behenyl alcohol, non-polar oils such as liquid paraffin and squalane, ester oils such as isopropyl palmitate and isopropyl myristate, vegetable oils such as wheat germ oil and olive oil, trimethylsiloxysilicic acid And silicon compounds such as methylphenylpolysiloxane and fluorine compounds such as perfluoropolyether.

  As surfactants, fatty acid monoglycerides, polyoxyethylene fatty acid esters, polyoxyethylene alkyl ethers, polyglycerin fatty acid esters and other nonionic surfactants, benzalkonium chloride and other cationic surfactants, amphoteric surfactants, There are anionic surfactants. In addition, moisturizing and softening agents, antioxidants, astringents, whitening agents, antibacterial agents, anti-inflammatory agents, bactericides, antiallergic agents, UV absorbers, UV scattering agents, stabilizers, vitamins, enzymes, etc. Examples include ingredients such as quasi-drug raw material standards, cosmetic ingredient-specific combination ingredient standards, cosmetic raw material standards, Japanese pharmacopoeia, and food additive official standards.

  Although the compounding quantity of the composite aqueous gel with respect to the skin external preparation or cosmetics in this invention is not specifically limited, 0.0005-5 mass% is preferable at the dry solid weight in the whole quantity, such as a skin external preparation. . If the amount is less than 0.0005% by mass, the effect of the present invention may not be sufficiently obtained. On the other hand, if the amount exceeds 5% by mass, an improvement in the effect commensurate with the increase is not recognized. From this viewpoint, 0.001 to 3 mass% is more preferable.

Examples of the present invention will be described below.
Example 1
1 g of partially myristoylated chitosan pyrrolidone carboxylate (product name: PM-chitosan, manufactured by Pierce Co., Ltd.), which is an ionic amphiphilic polymer, was dispersed in 100 ml of purified water. The zeta potential of this partially myristoylated chitosan pyrrolidone carboxylate dispersion showed +80 mV. On the other hand, 2.5 g of a phospholipid copolymer (manufactured by NOF Corporation, trade name: lipid PMB), which is a zwitterionic polymer having a hydrophobic monomer, was dispersed and dissolved in 50 ml of purified water. The zeta potential of this phospholipid copolymer dispersion was -2 mV.

  At room temperature, the dispersion of the phospholipid copolymer was gradually added to the dispersion of the partially myristoylated chitosan pyrrolidone carboxylate while stirring with a stirrer, and then 3 g of dipropylene glycol, a polyhydric alcohol, was added. After the addition, the mixture was further uniformly dispersed at room temperature using a homomixer (5000 rpm × 2 minutes).

  After UV sterilization, the mixture was allowed to stand at room temperature for 24 hours to obtain a colorless translucent complex aqueous gel having thixotropic properties. As in Test Example 1 described later, the composition ratio of the ionic amphiphilic polymer and the amphoteric polymer was changed and the viscosity was measured with a B-type viscometer. It exhibited a viscosity of ˜370,000 (0.6 rpm) and was excellent in thixotropy. Further, the zeta potential of the composite aqueous gel obtained by changing the composition ratio of the ionic amphiphilic polymer and the amphoteric polymer was in the range of +30 mV to +60 mV.

(Test Example 1)
The composition ratio (weight ratio) of the partially myristoylated chitosan pyrrolidone carboxylate that is the ionic amphiphilic polymer of Example 1 and the phospholipid copolymer that is the amphoteric polymer was changed, and the respective composition ratios were changed. The viscosity in was measured, and the relationship between the composition ratio and the viscosity was confirmed. The result is shown in FIG. As is apparent from FIG. 1, the viscosity increased as the amount of phospholipid copolymer relative to the partially myristoylated chitosan pyrrolidone carboxylate was increased. When the composition ratio of the partially myristoylated chitosan pyrrolidone carboxylate and the phospholipid copolymer was about 1: 1.5, the viscosity exceeded 350,000 and became the highest. However, when the phospholipid copolymer was further increased from the composition ratio of 1: 1.5 and the weight ratio of the phospholipid copolymer was increased, the viscosity gradually decreased.

  On the other hand, instead of the phospholipid copolymer (phospholipid copolymer) that is a zwitterionic polymer having the hydrophobic monomer, a phospholipid copolymer that is a zwitterionic polymer having no hydrophobic monomer (Japan) When tested in the same manner using a product made from fats and oils, trade name: Lipidure HM), formation of a composite gel was recognized, but its gel-forming ability was not always sufficient. Therefore, the formation of the composite aqueous gel can be achieved only by the binding of the amino group of the chitosan derivative (partial myristoylated chitosan pyrrolidone carboxylate) and the anion site of the phospholipid copolymer which is a zwitterionic polymer. In addition, it was suggested that the hydrophobic bond between the hydrophobic group of the chitosan derivative and the hydrophobic group of the phospholipid copolymer is also important.

(Test Example 2)
In the preparation method as in Example 1, the influence of the carbon number of the acyl group, which is the hydrophobic group of the partially acylated chitosan pyrrolidone carboxylate, which is an ionic amphiphilic polymer, on the ability to form a composite aqueous gel was observed. . Specifically, among the partially acylated chitosan pyrrolidone carboxylates, partially caprylated chitosan pyrrolidone carboxylates having different carbon numbers (acyl group having 8 carbon atoms), partially capricated chitosan pyrrolidone carboxylates (acyl group carbons) 10), partially laurylated chitosan pyrrolidone carboxylate (acyl group having 12 carbon atoms), partially myristoylated chitosan pyrrolidone carboxylate (acyl group having 14 carbon atoms), and partially oleylated chitosan pyrrolidone carboxylate (acyl group) (18) were combined with a phospholipid copolymer (Nippon Yushi Co., Ltd., trade name: lipid PMB), which is an amphoteric polymer having a hydrophobic monomer.

The results are shown in Table 1.

  As is apparent from Table 1, any partially acylated chitosan pyrrolidone carboxylate once formed a composite gel. However, in the case of laurylated, myristoylated or oleylated chitosan pyrrolidone carboxylate having 12 or more carbon atoms, formation of a complex aqueous gel was performed very well, whereas capricated chitosan pyrrolidone carboxylic acid having 10 carbon atoms was obtained. The formation ability was slightly reduced with the salt, and the formation ability was further reduced with the caprylated chitosan pyrrolidone carboxylate having 8 carbon atoms. From this, it was suggested that the number of carbon atoms of the hydrophobic group is preferably 8 or more, and more preferably 12 or more for forming the composite aqueous gel.

(Test Example 3)
The composite aqueous gel obtained by the method of Example 1 was freeze-dried to prepare a white sponge and subjected to FT-IR analysis (reflection type). As a result, as shown in FIG. 2, in the composite gel, the peak of the amino group (1575 cm ⁻¹ ) in the molecule was shifted about 9 cm ⁻¹ to the high wavenumber side. On the other hand, although the peak was shifted to the high wave number side, no change was observed in the absorption peak intensity. From this, it was confirmed that the charged amino group of the chitosan derivative, which is an ionic amphiphilic polymer, and the phospholipid copolymer, which is a zwitterionic polymer, interact with each other.

  On the other hand, in the polyion complex formed in the presence of hyaluronic acid, the peak intensity was remarkably lowered, suggesting a strong binding property. This is because the anion sites in the zwitterionic group molecules of the zwitterionic polymer are selectively bound to the amino groups of the ionic amphiphilic polymer formed from the polyamine, but the crystal structure seen in the polyion complex is shown. Not suggested.

(Test Example 4)
In this test example, antibacterial evaluation was examined. MRSA (methicillin-resistant Staphylococcus aureus) was inoculated into the composite aqueous gel obtained by the method of Example 1, and antibacterial properties were evaluated from changes over time in the number of viable bacteria. On the other hand, it was compared with the case where MRSA was inoculated to a non-complexed chitosan derivative and a phospholipid copolymer, respectively, and inoculated to a composite aqueous gel. As shown in FIG. 3, when MRSA was inoculated into a phospholipid copolymer, MRSA could not be killed after 60 minutes, whereas MRSA was contacted with the conjugated aqueous gel. In that case, it could be killed in 20 minutes. Moreover, when MRSA was contacted with the chitosan derivative, it took 60 minutes to kill it. This indicates that the phospholipid copolymer does not have antibacterial properties, whereas the chitosan derivative has antibacterial properties. However, by combining the chitosan derivative with the phospholipid copolymer, the antibacterial property is further improved. It was confirmed to improve.

(Test Example 5)
The composite aqueous gel obtained by the method of Example 1 was allowed to coexist with 0.1% cetylpyridium chloride as a cationic fungicide. Even when a cationic bactericidal agent was included, no change over time was observed in the gel physical properties. MRSA was inoculated, and bactericidal properties were evaluated from changes in the number of viable bacteria over time, and as a result, they were killed 2 minutes after contact. The composite aqueous gel of Example 1 did not reduce the efficacy of the cationic fungicide and was excellent in stability. On the other hand, in the gel formed with the 1.0% carboxyvinyl polymer aqueous solution and potassium hydroxide which is a comparative example, the remarkable effectiveness fall of the cationic disinfectant and the viscosity fall of the gel were recognized.

(Test Example 6)
The gelled preparation containing the complexed aqueous gel of Example 1 was tested for stability, feeling of use, drug efficacy, and the like. The following gelled preparation of Formulation Example 1 containing the complexed aqueous gel of Example 1 was prepared, and a 5% carboxyvinyl polymer solution and a 5% hydroxypropylcellulose solution were blended in place of the complexed aqueous gel. These were designated as Comparative Examples 1 and 2.

[Prescription Example 1]
Ingredient Amount (wt%)
(1) Composite aqueous gel of Example 1 (viscosity: 370000 cps) 35.0
(2) Dipropylene glycol 3.0
(3) Titanium oxide 3.0
(4) Benzalkonium chloride 0.1
(5) Berberine chloride 0.1
(6) Glycolic acid 0.5
(7) Sodium hydroxide 0.04
(8) Paraben 0.05
(9) Purified water remaining amount Add the combined aqueous gel of (1) to the heat-treated water phase composition of (2) to (9), uniformly disperse, and let it stand for 24 hours to gel. A gelled preparation (viscosity: 100,000 cps, pH: 4.0) was prepared.

[Comparative Example 1]
Ingredient Amount (wt%)
(1) 5% carboxyvinyl polymer solution 20.0
(2) Dipropylene glycol 3.0
(3) Titanium oxide 3.0
(4) Benzalkonium chloride 0.1
(5) Berberine chloride 0.1
(6) Glycolic acid 0.5
(7) Sodium hydroxide 0.2
(8) Paraben 0.05
(9) Purified water remaining amount The gel of (1) is added to the heat-treated water phase composition of (2) to (9), uniformly dispersed, and allowed to stand for 24 hours to be gelled. (Viscosity: 90000 cps, pH: 6.7) was prepared.

[Comparative Example 2]
Ingredient Amount (wt%)
(1) 5% hydroxypropylcellulose solution 20.0
(2) Dipropylene glycol 3.0
(3) Titanium oxide 3.0
(4) Benzalkonium chloride 0.1
(5) Berberine chloride 0.1
(6) Glycolic acid 0.5
(7) Sodium hydroxide 0.08
(8) Paraben 0.05
(9) Purified water remaining amount The gel of (1) is added to the heat-treated water phase composition of (2) to (9), uniformly dispersed, and allowed to stand for 24 hours to be gelled. (Viscosity: 30000 cps, pH: 5.3) was prepared.

With respect to the gelled preparations of the above Formulation Example 1, Comparative Example 1, and Comparative Example 2, the change with time in two freeze-thaw treatments for one month under the conditions of 40 ° C. and 45 ° C. was determined from changes in appearance and viscosity physical properties. In addition, the feeling of use of skin and hair by 20 female monitors was tested. Furthermore, the effect reduction of cationic active ingredients such as benzalkonium chloride and berberine chloride was also tested. The results are shown in Table 2.

In Table 2, each symbol indicates the following items.
(1) Stability ○: No change in physical properties / appearance △: Some changes in physical properties / appearance ×: Changes in physical properties / appearance (2) Usability on skin and hair ○: Female 20% of respondents who answered that they were good: 50% or more △: The percentage of respondents who answered that they were good among 20 female monitors: 30-50%
×: Response rate answered as good among 20 female monitors: Less than 30% (3) Medicinal efficacy ○: No decrease in effectiveness of cationic active ingredients (benzalkonium chloride, berberine chloride) △: Cationic Slight decrease in effectiveness of active ingredients (benzalkonium chloride, berberine chloride) ×: Decrease in effectiveness of cationic active ingredients (benzalkonium chloride, berberine chloride)

  As is clear from Table 2, the stability of the gelled preparation of Formulation Example 1 was higher than that of Comparative Examples 1 and 2, and almost no decrease in appearance and viscosity was observed. Moreover, as for the feeling of use by a female monitor, the gelled preparation of Formulation Example 1 gave better results than Comparative Examples 1 and 2. Furthermore, in Comparative Example 1, a decrease in the effect of the cationic active ingredient was observed, but in Formulation Example 1, it was not observed.

(Test Example 7)
Tests on skin viscoelasticity improvement and wrinkle improvement were conducted. A lotion was applied to the outer side of the forearm of 20 men and women (average age: 38.5 years old) for 6 weeks (twice / day). At the time of measurement, it was measured after rinsing for 20 minutes in an environment of 22 ° C. and 50% humidity. Skin viscoelasticity was determined using a cut meter (C / K) and stratum corneum water content was determined using SKIKON-200EX (manufactured by IBI S). Further, after collecting a replica of the evaluation site, a shadow generated by halogen lamp irradiation was taken into a CCD camera (manufactured by Keyence Corporation), and the average depth of wrinkles was determined by image analysis. The test results are shown in Table 3.

In Table 3, each symbol indicates the following items.
(1) Skin viscoelasticity ◎: The improvement rate shows 20% or more ○: The improvement rate shows 10-20% △: The improvement rate shows 5-10% ×: No improvement action is recognized (2) Angle Layer moisture content ◎: The improvement rate shows 40% or more ○: The improvement rate shows 20-40% △: The improvement rate shows 10-20% ×: No improvement effect is observed (3) Wrinkle improvement ◎: Average wrinkle depth is reduced by 30% or more ○: Average wrinkle depth is reduced by 20-30% △: Average wrinkle depth is reduced by 10-20% ×: No improvement effect is observed

  As is clear from Table 3, Formulation Example 1 of the present invention was superior in skin viscoelasticity, skin moisture content, and wrinkle improving action as compared with Comparative Examples 1 and 2. In addition, it was suggested that the gel preparation of Formulation Example 1 has a high skin care effect in the preparation itself, and safely enhances the anti-aging action of glycolic acid, which is a chemical peeling agent.

(Test Example 8)
The gelled preparation of Formulation Example 1 and the gelled preparation of Comparative Example 2 are applied to a human skin model (Toyobo Co., Ltd., TESTSKIN LSE003), reacted at 37 ° C. for 24 hours, and serve as an index of irritation released into the medium. The cytokine IL-1α was quantified by ELISA. It was estimated that the complexed gel of the above Formulation Example 1 relieves the irritation of the cationic drug and has a sustained release DDS action.

[Prescription Example 2]
This formulation example is a gel cream formulation example, and its composition is as follows.
Ingredient Amount (wt%)
(1) Cetanol 3.0
(2) Isopropyl myristate 3.0
(3) Mineral oil 8.0
(4) POE (25) sorbitan monolaurate 3.5
(5) Composite aqueous gel of Example 1 (viscosity: 370000 cps) 25.0
(6) Glycerin 9.0
(7) Dipropylene glycol 3.0
(8) Methylparaben 0.1
(9) Remaining amount of purified water Emulsified by homomixer treatment (7000 rpm, 1 minute) by adding the heat-dissolved aqueous phase composition of (5) to (9) to which the parts (1) to (4) were heated and dissolved Thus, a gel cream gel preparation (viscosity: 800,000 cps, pH: 4.3) was prepared.

[Prescription Example 3]
This formulation example is a formulation example of cloudiness essence lotion, and its composition is as follows.
Ingredient Amount (wt%)
(1) Liquid paraffin 1.0
(2) Isopropyl myristate 1.0
(3) POE (25) sorbitan monolaurate 3.0
(4) Composite gel of Example 1 (viscosity: 250,000 cps) 10.0
(5) Butylene glycol 5.0
(6) Licorice flavonoid 0.2
(7) Citrulline 2.0
(8) Ectoin 0.8
(9) Hydrogenated soybean phospholipid 0.5
(10) Remaining amount of purified water The water phase composition of (4) to (10) that has been heat-treated is heated and dissolved in parts (1) to (3), and emulsified by homomixer treatment to give a cloudy viscosity. An essence lotion was prepared.

[Prescription Example 4]
This prescription example is a prescription example of a gel-like hair treatment, and its composition is as follows.
Ingredient Amount (wt%)
(1) Ceramide 3 0.5
(2) Polymethylsiloxane 3.0
(3) Polyglycerol fatty acid ester 1.0
(4) Cetanol 1.0
(5) Composite aqueous gel of Example 1 (viscosity: 350,000 cps) 25.0
(6) Butylene glycol 5.0
(7) Ectoin 0.2
(8) Benzalkonium chloride 0.5
(9) Fine particle titanium oxide 1.0
(10) Remaining amount of purified water Emulsified by homomixer treatment (7000 rpm, 1 minute) after adding heat-dissolved parts (1) to (4) to the heat-treated water phase composition of (5) to (10) Thus, a gel cream gel preparation (viscosity: 800,000 cps, pH: 4.3) was prepared.

[Prescription Example 5]
This prescription example is a prescription example of an aqueous gel foundation, and its composition is as follows.
Ingredient Amount (wt%)
(1) Olive oil 0.5
(2) Polymethylsiloxane 3.0
(3) Titanium oxide 7.0
(4) Iron oxide 0.8
(5) Talc 2.5
(6) Bengala 0.7
(7) Composite aqueous gel of Example 1 (viscosity: 250,000 cps) 45.0
(8) Butylene glycol 5.0
(9) Ectoin 0.2
(10) Benzalkonium chloride 0.5
(11) Fine particle titanium oxide 1.0
(12) Remaining amount of purified water Emulsified by homomixer treatment (7000 rpm, 1 minute) by adding heat-dissolved parts (1) to (2) to the heat-treated water phase composition of (3) to (12) A gel-like foundation preparation (viscosity: 900,000 cps, pH: 4.2) was prepared.

  The composite aqueous gel of the present invention can be widely applied to external preparations for skin, cosmetics, and various other gelled preparations.

The graph which shows the relationship between the composition ratio of a chitosan derivative and a phospholipid copolymer, and composite gel viscosity. FT-IR chart of composite gel. The graph which shows the bactericidal action with respect to MRSA. The graph which shows stability of the composite gel containing a cationic chemical | medical agent.

Claims (18)

  A composite aqueous gel comprising an ionic amphiphilic polymer and an amphoteric polymer.   2. The composite aqueous gel according to claim 1, wherein the ionic amphiphilic polymer is obtained by partially introducing 0.1 to 50.0% of a hydrophobic group having 8 to 22 carbon atoms.   The composite aqueous gel according to claim 1 or 2, wherein the ionic amphiphilic polymer has an amino group.   The complex aqueous gel according to any one of claims 1 to 3, wherein the ionic amphiphilic polymer is chitin, chitosan, a chitin derivative, or a chitosan derivative.   The composite aqueous gel according to claim 4, wherein the chitin, chitosan, chitin derivative, or chitosan derivative is obtained by partially introducing 0.1 to 50.0% of a hydrophobic group having 8 to 22 carbon atoms.   The composite aqueous gel according to any one of claims 1 to 5, wherein the weight ratio of the ionic amphiphilic polymer to the amphoteric polymer is in the range of 1: 0.1 to 1: 10.0.   The composite aqueous gel according to any one of claims 1 to 6, wherein the zeta potential of the amphoteric polymer is -20 mV to +20 mV.   The complex aqueous gel according to any one of claims 1 to 7, wherein the amphoteric polymer has a phospholipid structure.   The complex aqueous gel according to any one of 1 to 7, wherein the amphoteric polymer has a phospholipid structure and a hydrophobic group.   10. A zeta potential at pH 3.5 to 5.5 in a state where an ionic amphiphilic polymer and an amphoteric polymer are complexed is 0 mV to +80 mV. Composite aqueous gel.   The composite aqueous gel according to any one of claims 1 to 10, wherein a polyhydric alcohol is contained in addition to the ionic amphiphilic polymer and the amphoteric polymer.   Production of a composite aqueous gel characterized by mixing an ionic amphiphilic polymer and an amphoteric polymer and then compounding the ionic amphiphilic polymer with the amphoteric polymer. Method.   The ionic amphiphilic polymer is mixed with the zwitterionic polymer so that the weight ratio of the ionic amphiphilic polymer to the zwitterionic polymer is in the range of 1: 0.1 to 1: 10.0. The method for producing a composite aqueous gel according to claim 12, wherein the composite is manufactured by compounding.   A gelled preparation comprising the composite aqueous gel according to any one of claims 1 to 11.   A skin external preparation comprising the composite aqueous gel according to any one of claims 1 to 11.   The skin external preparation according to claim 15, which contains a cationic component in addition to the complexed aqueous gel.   A cosmetic comprising the composite aqueous gel according to any one of claims 1 to 11.   The cosmetic according to claim 17, comprising a cationic component in addition to the composite aqueous gel.

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