JP2005274222A - Compost decay degree determination method and its device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a compost decay degree determination method and its device for simply, rapidly, and quantitatively determining the decay degree of compost. <P>SOLUTION: It is found out that a compost extract produced from compost emits faint light (biophotons) and that a correlation exists between the light amount of the faint light and the decay degree of the compost. According to this method, first, a compost extract is produced from compost. Subsequently, the number of photons emitted from the compost extract is measured by using a photomultiplier, etc. Then, the decay degree of the compost is determined based on a previously found correlation between the number of photons emitted from the compost extract and the decay degree of the compost. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a method and apparatus for determining the degree of maturity of compost.

  In recent years, recycling of waste has been promoted, so that there are increasing opportunities to compost and use raw materials that were not handled as compost raw materials. For this reason, it has become more important to suppress the destabilization of compost quality and the occurrence of unknown harmful effects. Furthermore, in order to ensure food safety, there is a need for a quick and accurate monitoring method for the quality of compost, which is a production material for agricultural products.

  One quality of compost is the maturity of compost. When immature compost, which has not progressed in maturity, is applied to soil, it causes indirect obstacles such as `` generation of high-concentration inorganic nitrogen '', `` abnormal reduction of soil '', `` nitrogen starvation '' due to rapid decomposition of organic matter in the soil. There is a risk that the growth of crops may be hindered by a direct hindrance effect due to the release of “growth inhibitory substances” and the like. For this reason, before applying compost to soil, it is preferable to grasp not only the compost components but also their maturity.

In addition, although the structure and objective of invention differ, there exists a disease resistance test method of the plant disclosed by patent document 1 as what measures the light-emission quantity from a plant, for example.
Japanese Patent No. 3231098

  Methods for determining the maturity of compost include “physical and chemical analysis methods” such as the weight measurement method of washing residue and the carbon ratio method, “on-site measurement methods” such as the color-specific method and the Nessler method, and There are several methods such as “judgment method”. However, these methods have the problems that the types of compost that can be determined are limited, the analysis work is complicated, and it takes a long time to obtain a result. At present, these results are comprehensively judged to determine the maturity level of compost. However, to accurately determine the maturity level of compost, the seedling test or germination test can be used. It is necessary to conduct bioassay tests using crops.

  However, bioassay tests such as the “plant seedling test” and “germination test” are test methods that use the process in which organisms are bred, so the test methods are complicated and it takes a long time to produce results. However, it is difficult to quantitatively evaluate the results. Therefore, there is a need for a method for determining the degree of maturity of compost capable of determining maturity simply and quickly and quantitatively.

  The present invention has been made in view of the above-described problems, and provides a compost maturity determination method and apparatus capable of determining the maturity of compost simply, quickly, and quantitatively. Objective.

  In order to solve the above-described problems, a method for determining the degree of maturity of compost according to the present invention is a method for determining the degree of maturity of compost, and includes an extract generation step for generating a compost extract from compost, and a compost extract. And a determination step for determining the degree of compost maturity based on a correlation between the amount of light emission from the sample and the maturity level of the compost.

  The present inventors have found that the compost extract emits faint light (biophoton), and that there is a correlation between the amount of the faint light and the maturity of the compost. According to the above method for determining the degree of maturity of compost, it is possible to determine the degree of maturity of compost using the above correlation by measuring the amount of luminescence from the sample containing the compost extract. In comparison, the maturity of compost can be determined much more easily and quickly. Further, according to the above-described method for determining the degree of maturity of compost, the degree of maturity of compost can be objectively determined on a quantitative scale such as the amount of light emitted from the sample.

  Moreover, the maturity determination method of compost may be characterized in that the sample is a compost extract. Thereby, as described above, the maturity of compost can be determined easily, quickly and quantitatively.

  Moreover, the maturity determination method of compost may be characterized in that the sample is a plant-derived sample that has come into contact with the compost extract. When the plant body sample is brought into contact with the compost extract, the plant body sample emits faint light (biophoton), and there is a correlation between the amount of the faint light and the maturity of the compost. Found it to exist. According to this method for determining the degree of maturity of compost, it is possible to determine the degree of maturity of compost using the above correlation by measuring the amount of luminescence from the plant-derived sample that has come into contact with the compost extract. And the maturity of compost can be determined quickly. Moreover, according to the above-described method for determining the degree of maturity of compost, the degree of maturity of compost can be objectively determined on a quantitative scale such as the amount of luminescence from the plant-derived sample.

  The compost maturity determination method is an extract that performs at least one of an acidification process, an alkalinization process, and an oxidation promotion process on the compost extract between the extract generation step and the measurement step. It may be characterized by further comprising a processing step. Thereby, the emitted light amount from a sample increases and the maturity of compost can be determined more correctly.

  Further, the compost maturity determination method may further include a constant temperature treatment step for performing a constant temperature treatment on the compost extract for a predetermined time between the extract generation step and the measurement step. Thereby, the emitted light amount from a sample increases and the maturity of compost can be determined more correctly.

  The compost maturity determination method may further include an ultraviolet irradiation step of irradiating the compost extract with ultraviolet rays between the extract generation step and the measurement step. Thereby, the emitted light amount from a sample increases and the maturity of compost can be determined more correctly.

  Further, the compost maturity determination apparatus according to the present invention is an apparatus for determining the maturity of compost, in the measurement means for measuring the amount of luminescence from the sample containing the compost extract produced from the compost, and the measurement means And determining means for determining the degree of maturity of compost based on the correlation between the measured amount of luminescence and the degree of maturity of compost. According to this compost maturity determination device, the measurement means measures the amount of light emitted from the sample containing the compost extract, and the determination means determines the maturity level of the compost using the above correlation. In addition, the maturity of compost can be determined quantitatively.

  Further, the compost maturity determination device may further include an ultraviolet irradiation means for irradiating the sample with ultraviolet rays. Thereby, the emitted light amount from a sample increases and the maturity of compost can be determined more correctly.

  According to the method and apparatus for determining the degree of maturity of compost according to the present invention, the degree of maturity of compost can be determined easily, quickly and quantitatively.

  DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments of a compost maturity determination method and apparatus according to the present invention will be described in detail with reference to the accompanying drawings. In the description of the drawings, the same elements are denoted by the same reference numerals, and redundant description is omitted.

(Embodiment)
First, terms used in the following description will be described. In the following description, “compost” is an agricultural production material used with the expectation of soil improvement effect and supply of fertilizer elements. In compost, organic materials such as livestock manure and agricultural by-products are aerobically fermented, easily degradable substances contained in the raw material are decomposed prior to soil application, and sufficient amounts of harmful substances contained in the water conditioning material are sufficient. It is preferably decomposed to reach a stable state.

  Also, “compost maturation” means that when the compost is applied to the soil, there is no obstacle to the growth of the crops, and the application of the compost gives energy to microorganisms in the soil and activates the activity. In other words, the organic matter in the compost is rotted in advance to such an extent that it is directly or indirectly linked to the maintenance of the geological force and does not cause adverse changes in the soil environment. And when it ripens to such a state, it is the end of ripening, that is, the ripening, and various degrees of ripening until reaching such a state are expressed by the term “ripening degree”.

  Next, an embodiment of a compost maturity determination apparatus according to the present invention will be described. FIG. 1 is a block diagram illustrating a configuration of a determination apparatus 1 that determines the degree of maturity of compost. Referring to FIG. 1, the determination device 1 includes a luminescence measurement device 2 and a determination means 10. The luminescence measuring apparatus 2 includes a sample tray 3, a photomultiplier tube 4, a photon counting circuit 5, a high voltage power supply 6, a voltage dividing circuit 7, a control circuit 9, and an ultraviolet light source 11.

  The sample tray 3 is a means for holding a sample containing a compost extract produced from compost, which is a target for determining the degree of maturity. The sample is preferably a compost extract itself or a plant-derived sample that has come into contact with the compost extract. Here, examples of the plant-derived sample include plant cells, plant tissues / sections, protoplasts, callus, and homogenates of plants and seeds. Moreover, it is preferable to use plant slices such as radish, sweet potato, and soybean. The sample tray 3 is placed in a dark room (not shown).

  The photomultiplier tube 4 and the photon counting circuit 5 constitute the measuring means in this embodiment. That is, the photomultiplier tube 4 is a means for detecting photons (photons) generated from the sample on the sample tray 3. The photomultiplier tube 4 is provided so that its photocathode faces the sample tray 3. The photomultiplier tube 4 converts photons from the sample into photoelectrons, current-amplifies the photoelectrons, and outputs the photons to the photon counting circuit 5. The photon counting circuit 5 is electrically connected to the anode of the photomultiplier tube 4 and receives an output current from the photomultiplier tube 4. The photon counting circuit 5 measures the amount of light emitted from the sample by integrating the number of photons incident on the photomultiplier tube 4.

  The high voltage power supply 6 generates a power supply voltage to be supplied to the photomultiplier tube 4. The voltage dividing circuit 7 generates a potential difference between the photocathode, each dynode, and the anode of the photomultiplier tube 4 by resistance-dividing the power supply voltage from the high-voltage power supply 6. The ultraviolet light source 11 is an ultraviolet irradiation means in this embodiment, and irradiates the sample on the sample tray 3 with ultraviolet rays. The sample is excited by being irradiated with ultraviolet rays from the ultraviolet light source 11.

  The control circuit 9 is means for controlling the high-voltage power supply 6 and the ultraviolet light source 11 described above. The control circuit 9 is connected to the determination means 10 via a communication line, and controls the operation / non-operation of the high-voltage power supply 6 and the ultraviolet light source 11 according to instructions from the determination means 10. The control circuit 9 is electrically connected to the photon counting circuit 5 and receives data relating to the amount of light emitted from the sample from the photon counting circuit 5. Then, the control circuit 9 sends data relating to the light emission amount to the determination means 10 through the communication line.

  The determination means 10 is a means for determining the degree of compost maturity based on the correlation between the amount of light emitted from the sample measured by the photomultiplier tube 4 and the photon counting circuit 5 and the degree of maturity of the compost. The determination unit 10 may be configured by, for example, a CPU executing a program stored in advance, or may be configured by an electronic circuit. The determination means 10 stores in advance a relational expression between the amount of luminescence from the sample and the degree of maturity of the compost, and determines the degree of compost maturity by applying data relating to the amount of luminescence from the control circuit 9 to the relational expression. To do. At this time, the determination means 10 calculates an estimated value such as a germination rate, a komatsuna root length, and a komatsuna germination delay in the Komatsuna germination test, which is an official method, as an index indicating the maturity of compost. Further, the determination means 10 calculates an estimated value such as moisture in the compost, nitrogen component in the compost, pH value of the compost, EC (electric conductivity of the compost extract) as an index indicating the maturity of the compost. May be. The correlation between the amount of luminescence from the sample and the maturity of the compost will be described in detail in a later example.

  Next, a method for determining the degree of maturity of compost according to this embodiment will be described, and the operation of the determination device 1 described above will be described. Here, FIG. 2A, FIG. 2B, FIG. 3A, FIG. 3B, and FIG. 4 are flowcharts showing first to fifth determination methods according to the present embodiment, respectively. .

First Determination Method The first determination method according to the present embodiment will be described with reference to FIG. First, a compost extract is generated. That is, the compost to be determined is dried and pulverized, placed in a container such as a flask, and boiling water is added and left for a predetermined time. And the compost extract is taken out by filtering the liquid in a flask (extracted liquid production | generation step, S11). Then, the compost extract produced | generated in step S11 is set to the sample tray 3 as a sample. Specifically, an appropriate amount of compost extract is dropped into a predetermined container, and the container is set in a recess provided at the center of the sample tray 3 (S12).

  Subsequently, the sample tray 3 is set in a dark room in the luminescence measuring device 2, and photons emitted from the compost extract are detected by the photomultiplier tube 4. Then, the number of photons incident on the photomultiplier tube 4 is integrated by the photon counting circuit 5, and the amount of light emitted from the compost extract is measured (measurement step, S13). Data on the measured light emission amount is sent to the determination means 10 via the control circuit 9.

  Subsequently, the maturity of the compost is determined by the determination means 10. That is, the determination means 10 applies data relating to the light emission amount received from the photon counting circuit 5 to a predetermined relational expression, and calculates estimated values such as a komatsuna germination rate, a komatsuna root length, and a komatsuna germination delay. Moreover, the determination means 10 calculates estimated values such as moisture in the compost, nitrogen component in the compost, pH value, and EC (determination step, S14).

Second Determination Method Next, the second determination method according to the present embodiment will be described with reference to FIG. In addition, since the extraction liquid production | generation step S21 in this method is the same as that of the extraction liquid production | generation step S11 of the 1st determination method mentioned above, description of this step is abbreviate | omitted.

  After the compost extract is generated in the extract generation step S21, the compost extract is dropped on the plant-derived sample. Specifically, for example, a plant slice having a cut surface formed on a plant body is prepared, and a compost extract is applied or dropped onto the cut surface (S22). In addition, as a plant used at this time, vegetables, such as a radish, a sweet potato, and a soybean, are suitable, for example.

  Subsequently, the plant-derived sample that has touched the compost extract in step S22 is set on the sample tray 3 (S23). Then, the sample tray 3 is set in a dark room in the light emission measuring device 2, and photons emitted from the plant-derived sample are detected in the photomultiplier tube 4. Then, the number of photons incident on the photomultiplier tube 4 is integrated by the photon counting circuit 5, and the light emission amount from the plant-derived sample is measured (measurement step, S24). Data on the measured light emission amount is sent to the determination means 10 via the control circuit 9. In the determination means 10, the maturity of the compost is determined in the same manner as in the first method described above (determination step, S25).

Third Determination Method Next, the third determination method according to the present embodiment will be described with reference to FIG. In addition, the extraction liquid production | generation step S31 in this method is the same as the extraction liquid production | generation step S11 of the 1st determination method mentioned above.

  After the compost extract is generated in the extract generation step S31, the compost extract is divided into a plurality of parts and left in a constant temperature environment at a substantially constant temperature for different times. As a specific example, the compost extract is divided into three, and one moves to the next step without taking time. The other one is left in a tank maintained at 35 ° C. for 24 hours and then moved to the next step. The other is left in a bath maintained at 35 ° C. for 48 hours and then moved to the next step. (Constant temperature treatment step, S32).

  Subsequently, the compost extract is set as a sample on the sample tray 3 (S33), and the amount of light emitted from the compost extract is measured (measurement step, S34). Data on the measured light emission amount is sent to the determination means 10. The determination means 10 determines the degree of maturity of the compost based on data relating to the amount of light emitted from each of the three compost extracts. For example, the determination means 10 determines the maturity level of the compost based on the difference or ratio of the two light emission amounts among the light emission amounts of the three divided compost extracts (determination step, S35).

  In this determination method, the compost extract is divided into a plurality of parts and left in a constant temperature environment for different times. However, the compost extract may be left in a constant temperature environment for a predetermined time without being divided.

Fourth Determination Method Next, the fourth determination method according to the present embodiment will be described with reference to FIG. In addition, the extraction liquid production | generation step S41 in this method is the same as the extraction liquid production | generation step S11 of the 1st determination method mentioned above.

After the compost extract is generated in the extract generation step S41, at least one of acidification, alkalinization, and oxidation promotion is performed on the compost extract. Specifically, when acidification is performed, an acidic solution such as HCl is added to the compost extract. Moreover, when performing an alkalinization process, alkaline solution, such as NaOH, is added to a compost extract. Further, when the oxidation-promoting treatment, the compost extract adding oxidizing promoting agent such as H ₂ O ₂ (extract process step, S42).

  Subsequently, the compost extract is set as a sample on the sample tray 3 (S43), and the amount of light emitted from the compost extract is measured (measurement step, S44). Data relating to the measured light emission amount is sent to the determination means 10, and the determination means 10 determines the maturity of the compost (determination step, S45).

Fifth Determination Method Next, the fifth determination method according to the present embodiment will be described with reference to FIG. In addition, the extraction liquid production | generation step S51 in this method is the same as that of the extraction liquid production | generation step S11 of the 1st determination method mentioned above.

  After the compost extract is generated in the extract generation step S51, the compost extract is irradiated with ultraviolet rays. That is, the compost extract is set as a sample in the sample tray 3 (S52), and the sample tray 3 is set in a dark room in the luminescence measuring device 2. Then, the compost extract is excited by irradiating the sample (compost extract) with ultraviolet rays from the ultraviolet light source 11 of the luminescence measuring device 2 for a predetermined time (ultraviolet irradiation step, S53). Subsequently, the amount of luminescence from the compost extract is measured (measuring step, S54). Data relating to the measured light emission amount is sent to the determination means 10, and the determination means 10 determines the maturity of the compost (determination step, S55).

  The first to fifth determination methods described above may be implemented in combination with each other. For example, the amount of light emitted from a sample containing a compost extract subjected to a constant temperature treatment step, the amount of light emitted from a sample containing a compost extract subjected to an extract treatment step, and a sample containing a compost extract subjected to an ultraviolet irradiation step The maturity of compost may be determined based on the amount of luminescence and the amount of luminescence from the plant-derived sample that has come into contact with the compost extract. Alternatively, the compost extract produced in the extract production step is subjected to an extract treatment step, a constant temperature treatment step, and an ultraviolet irradiation step, and based on the amount of light emitted from the sample containing the compost extract. You may determine the maturity of compost.

  The first determination method according to the present embodiment described above has the following effects. That is, as shown in the examples described later, the present inventors emitted faint light (biophoton) from a sample containing compost extract (in this determination method, compost extract itself). We found that there was a correlation between the amount of light and the maturity of compost. Therefore, according to the first determination method according to the present embodiment, a compost extract is generated from the compost to be determined in step S11, and the amount of luminescence from the sample containing the compost extract is measured in measurement step S13. By determining the maturity level of the compost using the correlation in step S14, the maturity level of the compost can be determined easily and quickly. In addition, according to the first determination method of the present embodiment, the maturity of compost can be objectively determined on a quantitative scale such as the amount of luminescence.

  Further, in the compost maturity determination method, the sample may be a plant-derived sample that has come into contact with the compost extract, as in the second determination method of the present embodiment. The present inventors have found that such a plant-derived sample emits faint light (biophoton), and there is a correlation between the amount of the faint light and the maturity of compost. Therefore, according to the 2nd determination method by this embodiment, the compost extract is produced | generated from the compost of determination object in step S21, and the light-emission quantity from the plant-derived sample which touched this compost extract is measured in step S24. By measuring and determining the maturity level of the compost using the above correlation in the determination step S25, the maturity level of the compost can be determined easily, quickly and quantitatively.

  Further, in the method for determining the degree of maturity of compost, as in the third determination method of the present embodiment, a constant temperature treatment is performed on the compost extract for a predetermined time between the extract generation step S31 and the measurement step S34. It is preferable to include a constant temperature processing step S32. Thereby, as shown in the Example mentioned later, the emitted light amount from a sample increases and it can determine the maturity of compost more correctly.

  In addition, the compost maturity determination method performs acidification treatment, alkalinization treatment, or oxidation promotion treatment between the extract generation step S41 and the measurement step S44 as in the fourth determination method of the present embodiment. It is preferable to provide an extract processing step S42 performed on the compost extract. Thereby, as shown in the Example mentioned later, the emitted light amount from a sample increases and it can determine the maturity of compost more correctly.

  Moreover, the maturity determination method of compost is the ultraviolet irradiation step which irradiates a compost extract with an ultraviolet-ray between extraction liquid production | generation step S51 and measurement step S54 like the 5th determination method of this embodiment. It is preferable to include S52. Thereby, as shown in the Example mentioned later, the emitted light amount from a sample increases and it can determine the maturity of compost more correctly.

  Moreover, the determination apparatus 1 according to the present embodiment has the following effects. That is, according to the determination apparatus 1 according to the present embodiment, the photomultiplier tube 4 and the photon counting circuit 5 measure the light emission amount from the sample containing the compost extract, and the determination means 10 correlates the light emission amount and the maturity degree. Since the maturity of compost is determined using the relationship, the maturity of compost can be determined easily, quickly and quantitatively.

  Moreover, it is preferable that the determination apparatus 1 is provided with the ultraviolet light source 11 which irradiates a sample with an ultraviolet-ray like this embodiment. Thereby, as shown in the Example mentioned later, the emitted light amount from a sample increases and it can determine the maturity of compost more correctly.

  In each Example shown below, the example of the correlation with the light-emission amount from the sample containing a compost extract and the maturity of compost is shown. Note that the correlation between the light emission amount and the maturity applied in the determination steps S14, S25, S35, S45, S55 and the determination means 10 of the above embodiment is not limited to the following examples.

  First, as Example 1, the correlation between the amount of luminescence from a plant section touching the compost extract and the maturity of the compost was examined. In this example, in order to investigate the correlation between the amount of luminescence from the plant section and the maturity of the compost, three types of compost having different maturity levels (i.e., immature compost that has been judged to be insufficiently fermented, almost no fermentation) Three kinds of mature compost judged to be finished and commercially available compost generally sold as matured) were prepared. In this example, compost made from manure from dairy was used.

Production of Compost Extract In this example, a compost extract was produced by the same method as the hot water extract used in the seedling assay method. That is, each of immature compost, final mature compost, and commercial compost is made into air-dried compost, 5 g of air-dried compost is placed in a 200 ml Erlenmeyer flask, 100 ml of boiling water (90 ° C. or higher) is added, and then the Erlenmeyer flask is made into an Erlenmeyer flask. Covered. And after leaving this for 1 hour, the double gauze was passed through the liquid in the Erlenmeyer flask and filtered. The liquid thus filtered was used as an immature compost extract, a final compost extract, and a commercial compost extract, respectively. In addition, when there is a time interval between the generation of these compost extracts and the luminescence measurement, the compost extract is preferably stored frozen.

As a preparation for investigating the correlation between the amount of luminescence from the compost extract measured after the germination test and the degree of maturity of the compost, the previously produced immature compost extract, final mature compost extract, and commercial compost extract were used. The germination test of the seedling test method, which is an index of the maturity of compost, was conducted. That is, immature compost extract, final compost extract, and commercially available compost extract were each dispensed 10 ml in a petri dish on which double filter paper had been previously laid. Then, 50 seeds of Komatsuna were sown from above, and the petri dish was capped and allowed to stand at 25 ° C. for 3 days, and then the germination rate of Komatsuna was investigated. In this example, the above operation was repeated three times, and the average value was taken as the germination rate of Komatsuna. As a result, the germination rate in the immature compost extract was 30.6%, the germination rate in the final compost extract and the germination rate in the commercial compost extract were 100%, respectively.

Measurement of luminescence amount First, a plant slice was prepared. That is, three radish cut into 1 cm square (thickness 5 mm), sweet potato cut into 1 cm square (thickness 5 mm), and soybean cut into half of one seed were prepared. In addition, preparation of these plant sections was performed 5 minutes before measuring the amount of luminescence. Thereafter, each plant section was placed in a 25 mm stainless steel dish, and 1 ml each of an immature compost extract, a final compost extract, and a commercially available compost extract was dropped on the surface (cut surface) of each of the three sections of each plant. At this time, the temperature of each compost extract was unified to 25 ° C., which is most suitable for the growth of vegetables in the soil. The petri dish thus prepared was placed on the sample tray 3 (see FIG. 1) and set in the luminescence measuring device 2, and the number of photons emitted from each plant section (that is, the amount of luminescence) was measured for 80 minutes.

  5 to 7 are graphs showing temporal changes in the number of photons emitted from the radish slice, sweet potato slice, and soybean slice, respectively. The graphs G1, G4, and G7 in these figures show the amount of light emitted from the slices dropped with the immature compost extract, and the graphs G2, G5, and G8 show the shots from the slice dropped with the final compost extract. The graphs G3, G6, and G9 show the amount of light emitted from the section where the commercial compost extract was dropped. Moreover, FIG. 8 is a table | surface which shows the number of photons for 80 minutes in each of a radish slice, a sweet potato slice, and a soybean slice. In FIG. 8, the numerical value in parentheses is a numerical value obtained by normalizing the number of photons, where the number of photons emitted from the section into which the commercial compost extract is dropped is 100.

  As shown in FIGS. 5 to 7, for the radish slice and the sweet potato slice, the number of photons (the amount of luminescence) emitted from the slice dropped with the immature compost extract increased with time. For soybean slices, the number of photons emitted decreased with time regardless of whether the immature compost extract, the final compost extract, or the commercial compost extract was dripped, but the immature compost extract was dripped. The number of photons emitted from the sections was higher than the number of photons emitted from the sections dropped with other compost extracts in all time zones. Moreover, as shown in FIG. 8, in any plant section, the number of photons emitted from the sections dropped with immature compost extract was higher than the number of photons emitted from the sections dropped with other compost extracts. The correlation values between the number of photons emitted from each of the radish slice, sweet potato slice, and soybean slice and the germination test result (komatsuna germination rate) were 0.85 for radish slice, 0.91 for sweet potato slice, and 0 for soybean slice. .90, a fairly high positive correlation was found.

  By the present Example described above, it was found that there is a correlation between the number of photons emitted from the plant sections touched with the compost extract (that is, the amount of luminescence) and the maturity of the compost.

  Next, as Example 2, the correlation between the amount of light emitted from the compost extract and the maturity of the compost was examined. In this example, immature compost, final matured compost, and commercially available compost in Example 1 were used in the same manner. Moreover, since the production | generation method of these compost extracts and the germination test method are the same as that of the said Example 1, detailed description is abbreviate | omitted.

Measurement of luminescence amount Prepare three stainless steel petri dishes each containing 1 ml of immature compost extract, final compost extract, and commercial compost extract, and place these petri dishes one by one on the sample tray 3 in the luminescence measuring device 2. The number of photons emitted from the compost extract was measured for each compost extract for 60 minutes.

  Fig.9 (a) is a graph which shows the time change of the photon number discharge | released from each compost extract. Graphs G10, G11, and G12 in the figure respectively indicate the number of photons emitted from the immature compost extract, the number of photons emitted from the mature compost extract, and the number of photons emitted from the commercial compost extract. FIG. 9B is a graph showing the number of photons emitted from each of the immature compost extract, the final compost extract, and the commercial compost extract for 60 minutes.

  As shown in FIG. 9 (a) and FIG. 9 (b), the number of photons emitted from the immature compost extract is larger than the number of photons emitted from other compost extracts in all time zones. The total number of photons emitted from the liquor was also higher than the total number of photons emitted from other compost extracts. The correlation value between the total number of photons emitted in this example and the germination test result (komatsuna germination rate) was −0.97, and a fairly high negative correlation was found.

  According to the present embodiment described above, it was found that there is a correlation between the number of photons emitted from the compost extract (that is, the amount of luminescence) and the maturity of the compost.

  Next, as Example 3, the correlation between the amount of luminescence from the compost extract produced from various compost raw materials and the maturity of the compost was examined. In Examples 1 and 2 above, the correlation between the amount of luminescence and maturity was investigated using compost made from manure from dairy farms, but the actual compost is not only dairy compost but also livestock excrement such as chicken manure. Compost derived from food by-products, pruning waste and household waste is also used. Therefore, in the present Example, the compost produced | generated from the above various compost raw materials was prepared. FIG. 10 is a table showing the sampling place, origin, producer evaluation of maturity, moisture, nitrogen component, pH value, and EC of the compost used in this example. As shown in FIG. 10, 40 types of compost having different origins and maturity levels were prepared in this example.

Production of compost extract Each of the 40 types of compost shown in FIG. 10 is air-dried and composted, 100 ml of distilled water is added to 5 g of air-dried compost, and the mixture is stirred at 60 ° C. for 3 hours, and then filtered through a mesh. An extract was produced. In addition, the compost extract was stored frozen at −10 ° C. until the compost extract was used for each test.

Germination test As a preparation for investigating the correlation between the amount of luminescence from the compost extract and the maturity of the compost, the germination test (germination rate, root length, Germination delay). In this example, each compost extract was divided into two before the germination test, and EC was adjusted to 4.0 by adding a phosphate buffer to one of the compost extracts, and the other compost extract was extracted. The EC of the liquid was not adjusted. FIG. 11 shows the germination test result without EC adjustment in this example, and FIG. 12 shows the germination test result after EC adjustment. 11 and 12 show the average value of the Komatsuna germination rate, the index and average value of the Komatsuna root length, and the delay rate and average value of the germination delay in each of the 40 types of compost extracts. In addition, the average value of germination delay has shown the number of individuals which did not germinate in 1 day-1.5 days among the seeds of 50 Komatsuna seeded.

Measurement of luminescence amount Each of the 40 types of compost extract was divided into two and divided into first and second groups. The first group was subjected to luminescence measurement immediately after thawing, and the second group was allowed to stand in an incubator at 35 ° C. for 24 hours (constant temperature treatment), and then subjected to luminescence measurement as follows. That is, 2 ml of the compost extract is put into an aluminum petri dish (outer diameter 38 mm, height 10 mm, thickness 1 mm), and compost is extracted under the conditions of a gate time of 1 second and a measurement time of 5 minutes in the luminescence measuring device 2 (see FIG. 1). The number of photons emitted from the liquid was measured.

  FIG. 13 is a table showing a correlation value between the germination rate, root length index, and germination delay index shown in FIGS. 11 and 12 and the number of photons emitted from the compost extract measured in the luminescence measurement. As shown in FIG. 13, there is a high correlation between the number of photons emitted from the EC non-adjusted compost extract subjected to isothermal treatment (35 ° C., 24 hours) and the germination rate, root length index, and germination delay index. The present inventors have found that there is. The present inventors have also found that there is a relatively high correlation between the number of photons emitted from the EC-adjusted compost extract subjected to the isothermal treatment and the germination rate. In FIG. 13, the relatively high correlation value is underlined. From this result, it can be seen that it is preferable to perform a constant temperature treatment step on the compost extract before performing the measurement step. In addition, this result is considered to be due to the fact that immature compost has a high ratio of easily decomposables, and therefore it is considered that decomposition was promoted under constant temperature (35 ° C) conditions, and from the difference in oxidation reaction accompanying decomposition at that time It is considered that the correlation as shown in FIG. 13 was obtained.

Further, based on a relatively high correlation value shown in FIG. 13, the correlation between the photon emission number X ₁ from the compost extract subjected to isothermal treatment and the germination rate Y ₁ in the Komatsuna germination test is as follows: It can be expressed as the following formula (1). Similarly, the correlation between the photon emission number X ₁ and the Komatsuna germination delay Y ₂ can be expressed as the following formula (2). Further, the correlation between the photon emission number X ₂ from the EC-adjusted compost extract subjected to the isothermal treatment and the Komatsuna germination rate Y ₃ after the EC adjustment can be expressed as the following formula (3). . Note that R in the formula is a multiple correlation.
[Equation 1]
Y ₁ = -59.7X ₁ +157.4 R = 0.68 (1)
[Equation 2]
Y ₂ = -86X ₁ +174.7 R = 0.66 (2)
[Equation 3]
Y ₃ = -33.3X ₂ +130.6 R = 0.66 (3)
In the embodiment described above, the determination step (determination means) estimates the germination rate and germination delay based on the correlation between the amount of luminescence from the compost extract formulated in this way and the degree of maturity of compost. The maturity of compost can be determined.

  Next, as Example 4, the correlation between the amount of luminescence from the compost extract irradiated with ultraviolet rays and the maturity of the compost was examined. In addition, the compost raw material in this embodiment was the same as that in Example 3 (see FIG. 10). Also in this example, as in Example 3 above, each compost extract was divided into two parts, and the EC was adjusted to 4.0 by adding a phosphate buffer to one of the compost extracts, The EC of the other compost extract was not adjusted.

Measurement of luminescence amount Each of 40 types of compost extracts without EC adjustment was divided into three and divided into first to third groups. The first group was immediately after thawing, the second group was left in an incubator at 35 ° C. for 24 hours, and the third group was left in an incubator at 35 ° C. for 48 hours. Luminescence measurement was performed as follows. That is, 2 ml of the compost extract was placed in an aluminum petri dish and set in the light emission measuring device 2, and then the compost extract was irradiated with ultraviolet light from the ultraviolet light source 11 for 5 seconds. Then, the number of photons emitted from the compost extract was measured under conditions such as a gate time of 1 second and a measurement time of 5 minutes. In addition, the number of photons emitted was also measured in the above procedure for each of the 40 types of compost extracts adjusted for EC.

FIG. 14 shows the following numerical values A _{1 to} A ₅ based on the root length index, root length average value, germination delay index, and germination rate shown in FIGS. 11 and 12, and the number of photons emitted from the compost extract. It is a table | surface which shows a correlation value. That is, assuming that the photon emission number immediately after thawing is a ₁ , the photon emission number after the 24-hour isothermal treatment is a ₂ , and the photon emission number after the 48-hour isothermal treatment is a ₃ , A ₁ = a ₂ −a ₁ , A ₂ = A ₃ -a ₁ , A ₃ = a ₂ / a ₁ , A ₄ = a ₃ / a ₁ , A ₅ = a ₃ / a ₂ .

As shown in FIG. 14, the amount of light emitted from the compost extract that has not been adjusted for EC and that has been subjected to constant temperature treatment (35 ° C., 48 hours) and ultraviolet irradiation treatment, and the germination rate without EC adjustment. A relatively high correlation was found (for example, numerical values A ₂ and A ₄ ). In addition, a relatively high correlation was found between the numerical value A ₅ and the root length index, root length average value, germination delay rate, and germination rate without EC adjustment. Further, a relatively high correlation was found between the numerical value A ₅ and the germination delay rate and germination rate after EC adjustment. In FIG. 14, the relatively high correlation value is underlined. This result shows that it is preferable to perform an ultraviolet irradiation step with respect to the compost extract before performing a measurement step.

FIG. 14 also shows correlation values between the compost analysis values (water, nitrogen component, pH value, and EC) and numerical values A _{1 to} A ₅ based on the number of photons emitted from the compost extract.

  Next, as Example 5, the correlation between the amount of luminescence from the compost extract subjected to acidification, alkalinization, or oxidation promotion and the maturity of the compost was examined. In addition, the compost raw material in this embodiment is 15 types (No. 2, 4, 10, 11, 13, 15, 18, 20, 20) selected based on the pH value in Example 3 (see FIG. 10). 22, 24, 25, 26, 30, 32, 34) and distilled water as a blank. Also in this example, as in Examples 3 and 4, each compost extract was divided into two, and the EC was adjusted to 4.0 by adding a phosphate buffer to one of the compost extracts. The EC of the other compost extract was not adjusted.

Measurement of luminescence amount 2 ml of compost extract immediately after thawing without EC adjustment is put in a styrene petri dish (outer diameter 40 mm, height 10 mm, thickness 1 mm), and luminescence measurement that can measure 15 types of compost extract continuously. After setting the petri dish on the apparatus, immediately before starting the light quantity measurement, each treatment agent (0.5 ml of 1N HCl for acidification treatment, 0.5 ml of NaOH for alkalinization treatment, 46 for oxidation promotion treatment) % H ₂ O ₂ 0.2 ml) was added, and the number of photons emitted from the compost extract was measured under the conditions of a gate time of 1 second and a measurement time of 80 minutes. In addition, the number of photons emitted from the EC-adjusted compost extract was also measured according to the above procedure.

FIGS. 15 to 17 show the case where the compost extract is subjected to an acidification treatment (FIG. 15), the compost extract is subjected to an alkalinization treatment (FIG. 16), and the compost extract is subjected to an oxidation promotion treatment. In each of (FIG. 17), the following numerical values B ₁ to B based on the root length index, root length average value, germination delay index, and germination rate shown in FIGS. 11 and 12 and the number of photons emitted from the compost extract it is a table showing a correlation value between B _7. 15 to 17, the significant correlation value is underlined with a double underline, and the relatively high correlation value is underlined.
B ₁ = (Total number of photons from compost extract for 80 minutes)
B ₂ = B ₁ − (total number of photons from blank for 80 minutes)
B ₃ = (total number of photons in 80 minutes from the compost extract) − (total number of photons in 80 minutes from the compost extract to which no treatment chemical solution is added)
B ₄ = B ₃ − (total number of photons from blank for 80 minutes)
B ₅ = logarithm of the absolute value of B ₄ B ₆ = (80-minute photon deviation from compost extract)
B ₇ = logarithm of the absolute value of B ₆

  In this example, a larger number of photon emission was obtained than in Example 3 in which the compost extract was subjected to constant temperature treatment by pH adjustment treatment or oxidation promotion treatment such as acidification treatment or alkalinization treatment. As shown in FIGS. 15 to 17, when pH adjustment treatment or oxidation promotion treatment is performed, not only bioassay values such as root length, germination delay and germination rate but also physicochemical analysis values such as moisture and nitrogen component amounts A relatively high correlation was also found. As described above, the compost extract produced from immature compost contains more readily degradable substances, so that the oxidation reaction, which is the main cause of photon emission, will be promoted under conditions where the chemical reaction is promoted. In this example, it is presumed that a part of the oxidation reaction was amplified by adjusting the pH value. Further, in this example, when the oxidation promotion treatment was performed, a larger photon emission number was obtained as compared with the case where the pH value was adjusted. Since the oxidation reaction accompanied by oxygen addition shows a continuous chain property, it is presumed that the oxidation reaction was amplified by the supply of a highly decomposable oxygen source.

Based on a relatively high correlation value among the correlation values shown in FIGS. 15 to 17, the correlation between the number of photons emitted from the compost extract (emission amount) and the maturity of the compost can be formulated. For example, the correlation between the photon emission number X ₃ from the EC-adjusted compost extract subjected to oxidation promotion treatment and the Komatsuna root length Y ₄ after the EC adjustment can be expressed as the following formula (4). it can. Further, the correlation between the photon emission number X ₄ from the EC non-adjusted compost extract subjected to the oxidation promotion treatment and the moisture amount Y _{5 of the} compost can be expressed as the following formula (5).
[Equation 4]
Y ₄ = -5.8X ₃ +39.1 R = 0.72 (4)
[Equation 5]
Y ₅ = 37X ₄ -122.9 R = 0.60 (5)
In the embodiment described above, the determination step (determination means) estimates the komatsuna root length and the amount of compost moisture based on the correlation between the number of photons emitted from the compost extract formulated in this way and the maturity of the compost. And the maturity of the compost can be determined.

  Next, as Example 6, the correlation between the number of photons emitted from the radish slices dropped with the compost extract and the maturity of the compost was examined. In addition, the compost raw material in this embodiment was made into 20 types (No. 1-5, 7-15, 18-21, 24) among the said Example 3 (refer FIG. 10). Also in this example, as in Examples 3-5 above, each compost extract was divided into two, and the EC was adjusted to 4.0 by adding a phosphate buffer to one of the compost extracts. The EC of the other compost extract was not adjusted.

Measurement of luminescence amount The middle part of the blue neck radish was sliced to a thickness of about 3 mm and then formed into a disk shape having a diameter of 36 mm to prepare a radish slice. After putting this in the styrene petri dish of Example 5, 0.15 ml of non-EC-adjusted compost extract was dropped on the cut surface of the radish section and left in an incubator at 20 ° C. Then, the number of photons emitted from the radish slices was measured under the conditions of 1 hour, 2 hours, 3 hours, 4 hours, and 24 hours after dropping the compost extract, with a gate time of 10 seconds and a measurement time of 3 minutes. . In addition, the number of photons emitted from the EC-adjusted compost extract was also measured according to the above procedure. In the measurement after 24 hours, the number of photons emitted from the front and back surfaces of the disc-shaped radish slice was measured.

  FIG. 18 shows the root length index, root length average value, germination delay index, and germination rate shown in FIGS. 11 and 12, and the number of photons emitted from the radish slice when the compost extract was dropped on the radish slice. Is a table showing the respective correlation values of 1 hour, 2 hours, 3 hours, 4 hours, 24 hours (section surface), and 24 hours (section back surface) after dropping of the compost extract. In FIG. 18, the relatively high correlation value is underlined.

  In this example, the number of photons obtained from the radish slices to which the compost extract was dropped was larger than the number of photons from the compost extract subjected to isothermal treatment, and a change in the number of photons appeared in a short time. A relatively high correlation was found between the photon number from the radish slice and the bioassay value 24 hours after the dropping, and a higher correlation was found 1 hour after the dropping. This is presumed to be due to the fact that the decomposition of undegraded substances in the compost was promoted by the enzymes contained in the tissue fluid of the plant body, the oxidation reaction was amplified, and the like. Moreover, it is guessed that the oxidation reaction was accelerated | stimulated by the stress reaction of the plant (radish slice) with respect to the harmful substance in a compost extract.

Based on a relatively high correlation value shown in FIG. 18, the correlation between the number of photons emitted from the radish slice and the degree of maturity of compost can be formulated. For example, the correlation between the photon emission number X ₅ from the radish slice dripped with the EC non-adjusted compost extract, the Komatsuna germination rate Y ₁ , the Komatsuna germination delay Y ₂ , and the Komatsuna root length Y ₆ is as follows: It can represent like Numerical formula (6)-(8). Further, the correlation between the photon emission number X ₅ from the radish section and the nitrogen component Y ₇ , pH value Y ₈ , and ECY ₉ can be expressed as the following mathematical formulas (9) to (11), respectively.
[Equation 6]
Y ₁ = 68.9X ₅ −42.6 R = 0.62 (6)
[Equation 7]
Y ₂ = 98.3X ₅ -111.8 R = 0.67 (7)
[Equation 8]
Y ₆ = 10.6X ₅ -15.4 R = 0.69 (8)
[Equation 9]
Y ₇ = 3.1X ₅ -5.9 R = 0.64 (9)
[Equation 10]
Y ₈ = −3.4X ₅ +17.4 R = 0.65 (10)
[Equation 11]
Y ₉ = −3.7X ₅ +25.3 R = 0.58 (11)
In the embodiment described above, the determination step (determination means) estimates the bioassay value and the physicochemical analysis value based on the correlation between the number of photons emitted from the plant section and the maturity of the compost formulated in this way. And the maturity of the compost can be determined.

  Next, as Example 7, the correlation between the number of photons emitted from the sweet potato slices dropped with the compost extract and the maturity of the compost was examined. In addition, the compost raw material in this embodiment is the same as that of the said Example 6. FIG. Also in this example, as in Examples 3 to 6, each compost extract was divided into two parts, and the EC was adjusted to 4.0 by adding a phosphate buffer to one of the compost extracts. The EC of the other compost extract was not adjusted.

Measurement of Luminescence The middle part of sweet potato (red golden) was sliced to a thickness of about 3 mm and then formed into a disk shape having a diameter of 36 mm to prepare a sweet potato slice. After putting this in a styrene petri dish, 0.30 ml of an unadjusted compost extract was dropped onto the cut surface of the sweet potato slices and left in an incubator at 20 ° C. Then, after 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, 12 hours, and 24 hours after dropping of the compost extract, the sweet potato slices were measured under the conditions of a gate time of 10 seconds and a measurement time of 3 minutes. The number of photons emitted from was measured. In addition, the number of photons emitted from the EC-adjusted compost extract was also measured according to the above procedure. In the measurement after 24 hours, the number of photons emitted from the front and back surfaces of the disk-shaped sweet potato slices was measured.

  FIG. 19 shows the root length index, root length average value, germination delay index, and germination rate shown in FIGS. 11 and 12, and the number of photons emitted from the sweet potato slice when the compost extract was dropped on the sweet potato slice. Correlation values of 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, 12 hours, 24 hours (section surface), and 24 hours (section back surface) after dropping the compost extract It is a table | surface shown about each of these. In FIG. 19, the significant correlation value is underlined and the relatively high correlation value is underlined.

  In this example, the number of photons obtained from the sweet potato slices to which the compost extract was dropped was larger than that in Example 6 (radish slice). The time until the change in the number of photons appeared was faster in Example 6. In addition, a significant correlation was found between the photon number from the sweet potato slice and the bioassay value 24 hours after the dropping.

Based on a significant correlation value or a relatively high correlation value shown in FIG. 19, the correlation between the number of photons emitted from the sweet potato slice and the maturity of the compost can be formulated. For example, the correlation between the photon emission number X ₆ from a sweet potato slice dripped with an EC non-adjusted compost extract and the Komatsuna germination rate Y ₁ , Komatsuna germination delay Y ₂ , Komatsuna root length Y ₆ , and pH value Y ₈ These can be expressed as the following mathematical formulas (12) to (15). Moreover, the correlation between the photon emission number X ₇ from the sweet potato slices dropped with the EC adjusted compost extract and the Komatsuna germination rate Y ₃ , Komatsuna root length Y ₄ , and Komatsuna germination delay Y ₁₀ after EC adjustment. Can be expressed as the following mathematical formulas (16) to (18), respectively.
[Equation 12]
Y ₁ = −0.004X ₆ +115.6 R = 0.69 (12)
[Equation 13]
Y ₂ = −0.004X ₆ +58.9 R = 0.65 (13)
[Formula 14]
Y ₆ = −0.0054X ₆ +112.5 R = 0.73 (14)
[Equation 15]
Y ₈ = −0.0008X ₆ +9.4 R = 0.61 (15)
[Equation 16]
Y ₃ = −0.002X ₇ +104.9 R = 0.60 (16)
[Equation 17]
Y ₄ = −0.008X ₇ +114.2 R = 0.76 (17)
[Equation 18]
Y ₁₀ = 0.0008X ₇ +0.95 R = 0.65 (18)
In the embodiment described above, the determination step (determination means) estimates the bioassay value and the physicochemical analysis value based on the correlation between the number of photons emitted from the plant section and the maturity of the compost formulated in this way. And the maturity of the compost can be determined.

It is a block diagram which shows the structure of the determination apparatus which determines the maturity of compost. (A) It is a flowchart which shows the 1st determination method. (B) It is a flowchart which shows the 2nd determination method. (A) It is a flowchart which shows the 3rd determination method. (B) It is a flowchart which shows the 4th determination method. It is a flowchart which shows the 5th determination method. In Example 1, it is a graph which shows the time change of the number of photons emitted from the radish section. In Example 1, it is a graph which shows the time change of the number of photons emitted from the sweet potato slice. In Example 1, it is a graph which shows the time change of the number of photons emitted from the soybean section. In Example 1, it is a table | surface which shows the total number of the photons emitted from the radish section, the sweet potato section, and the soybean section. In Example 2, (a) The graph which shows the time change of the number of photons emitted from each of an immature compost extract, a final mature compost extract, and a commercial compost extract, and (b) a graph which shows the total number. It is a table | surface which shows the sampling place of origin of the compost used for Example 3, origin, producer evaluation of a maturity degree, a water | moisture content, a nitrogen component, pH value, and EC. It is a germination test result in EC unadjusted in Example 3. It is a germination test result after EC adjustment in Example 3. In Example 3, it is a table | surface which shows the correlation value with the germination rate shown in FIG.11 and FIG.12, a root length index | exponent, a germination delay index | exponent, and the light-emission quantity from a compost extract. In Example 4, the root length index, root length average value, germination delay index, and germination rate shown in FIGS. 11 and 12 and the correlation values of numerical values A _{1 to} A ₅ based on the amount of luminescence from the compost extract are shown. It is a table | surface which shows. In Example 5, when the compost extract was acidified, the root length index, the root length average value, the germination delay index, and the germination rate shown in FIGS. 11 and 12 and the luminescence from the compost extract is a table showing the correlation between the numerical B ₁ .about.B ₇ based on the amount. In Example 5, when the compost extract was subjected to alkalinization treatment, the root length index, root length average value, germination delay index, and germination rate shown in FIGS. 11 and 12, and luminescence from the compost extract is a table showing the correlation between the numerical B ₁ .about.B ₇ based on the amount. In Example 5, when the compost extract was subjected to oxidation promotion treatment, the root length index, the root length average value, the germination delay index, and the germination rate shown in FIGS. 11 and 12, and luminescence from the compost extract is a table showing the correlation between the numerical B ₁ .about.B ₇ based on the amount. When the compost extract is dropped on the radish slice, the root length index, root length average value, germination delay index, and germination rate shown in FIGS. 11 and 12 are correlated with the number of photons emitted from the radish slice. It is a table | surface which shows. When the compost extract is dripped onto the sweet potato slices, the root length index, root length average value, germination delay index, and germination rate shown in FIGS. 11 and 12 are correlated with the number of photons emitted from the sweet potato slices. It is a table | surface which shows.

Explanation of symbols

  DESCRIPTION OF SYMBOLS 1 ... Determination apparatus, 2 ... Luminescence measuring apparatus, 3 ... Sample tray, 4 ... Photomultiplier tube, 5 ... Photon counting circuit, 6 ... High voltage power supply, 7 ... Voltage division circuit, 9 ... Control circuit, 10 ... Determination means, 11 ... UV light source.

Claims (8)

A method for determining the maturity of compost,
An extract generation step for generating a compost extract from the compost;
A measuring step for measuring the amount of luminescence from the sample containing the compost extract;
And a determination step of determining the degree of maturity of the compost based on the correlation between the amount of luminescence from the sample and the maturity of the compost.   The method according to claim 1, wherein the sample is the compost extract.   The method according to claim 1, wherein the sample is a plant-derived sample that has come into contact with the compost extract.   And further comprising an extract treatment step for performing at least one of acidification treatment, alkalinization treatment, and oxidation promotion treatment on the compost extract between the extract production step and the measurement step. The method for determining the degree of maturity of compost according to any one of claims 1 to 3.   The thermostat processing step which performs a thermostat process of the predetermined time with respect to the said compost extract is further provided between the said extract production | generation step and the said measurement step, It is any one of Claims 1-4 characterized by the above-mentioned. The method for determining the degree of maturity of compost as described in 1.   The method according to any one of claims 1 to 5, further comprising an ultraviolet irradiation step of irradiating the compost extract with ultraviolet rays between the extract generation step and the measurement step. A method for judging the maturity of compost. An apparatus for determining the maturity of compost,
Measuring means for measuring the amount of luminescence from the sample containing the compost extract produced from the compost,
A compost maturity determination device, comprising: a determination unit that determines the maturity level of the compost based on the correlation between the amount of luminescence measured by the measurement unit and the maturity level of the compost.   The compost maturity determination apparatus according to claim 7, further comprising ultraviolet irradiation means for irradiating the sample with ultraviolet rays.

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