JP2005261460A - Animal fixing base - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a fixing device for a small animal capable of performing ultrasonic irradiation stimulation under non-anesthesia. <P>SOLUTION: This animal fixing base is provided with a case body in a half bell shape, whose tip part is closed, provided with an opening part through which a mouse or a rat can intrude on the other end side, and provided with a fixing plate which can be installed in multiple stages for limiting the movement of the animal matched with the size on the case body on an opening part end side. The tip part of the case body in the half bell shape is light-shielded. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to an animal fixing table for fixing small animals. More specifically, the present invention relates to an animal fixing table used when administering a drug under non-anesthetized conditions to a small animal used for animal experiments such as rats and mice or conducting a treatment experiment using medical equipment such as ultrasonic irradiation. It is.

  2. Description of the Related Art Conventionally, when performing an animal experiment, an apparatus for fixing an animal when blood collection or surgery is performed has been developed. In the case of small and medium animals such as rabbits, there are those that are placed on a stand and only the head is fixed, and those that are placed in a case and only the neck are fixed. In addition, as a fixing device for preventing hip dislocation, a fixing device as described in JP 2000-197649 A has been proposed.

  On the other hand, for rodent animals such as rats and mice, in the case of subcutaneous or intraperitoneal administration, drugs are forcibly fixed by human hand, or for long-term administration experiments or surgery. Was performed under anesthesia. In addition, semi-bell shaped animal fixtures have been developed for tail vein administration for rats and mice.

  Even when performing animal experiments for physical therapy, for example, when performing ultrasonic irradiation experiments on small animals every day, conventionally, anesthesia of animals such as halothane anesthesia has been performed (Non-Patent Document 1, Non-Patent Documents). 2, Non-Patent Document 3).

JP 2000-197649 A Y Azuma et al: Low-intensity pulsed ultrasound accelerates rat femoral fracture healing by acting on the various cellular reactions in the fracture callus; Journal of Bone Mineral Research 268, p671-680,2000 Y Takakura et al: Low-intensity pulsed ultrasound enhances early healing of medial collateral ligament injuries in rats; Journal of Ultrasound in Medoline & Biology 21, p283-288,2002 S Takikawa et al: Low-intensity pulsed ultrasound initiates bone healing in rat nonunion fracture model; Nournal of Ultrasound in Medline & Biology 20, p197-205,2001

  However, when anesthesia is performed and an experiment such as ultrasonic irradiation is performed, the blood flow of the animal changes due to anesthesia, and the animal is subjected to a lot of stress. In many cases, animals are killed. In addition, when examining the effects of ultrasonic stimulation, it is difficult to obtain accurate information because various proteins and the like are changed only by applying stress to the animal. Unlike drug administration experiments, changes in biological parameters with respect to physicochemical stimuli such as ultrasonic irradiation are small and are easily affected by anesthesia and stress. Large animals such as rabbits are suitable for experiments because they are relatively gentle, but it is difficult to increase the number of experiments. To obtain statistical data, experiments with small animals such as rats and mice are required.

  The present invention is an animal fixing table that solves the above-mentioned problems. In small animal experiments, daily ultrasonic irradiation is possible without anesthesia, reducing stress on animals during anesthesia, This is an animal fixed table that can obtain accurate data for sonication stimulation.

  Therefore, the present inventors have conducted intensive studies on a method that enables ultrasonic irradiation stimulation to animals such as rats and mice without anesthesia, and as a result, have found the following animal fixation tables.

  That is, the present invention has a semi-bell-shaped housing with a closed end, an opening through which the mouse or rat can enter on the other end, and an animal on the housing on the opening end side. An animal fixing base having a fixing plate that can be installed in multiple stages that restricts its movement in accordance with the size of the animal, wherein the tip of the half bell-shaped housing is shielded from light. A fixed base is provided.

  Further, the present invention is characterized in that the light-shielding part is made of a light-shielding sponge material stretched on the inner wall of the housing, and the light-shielding part includes at least a rear part of the position of the eye of the animal The present invention provides an animal fixing table characterized by the above.

  The present invention also includes a fixing portion for an ultrasonic transducer for irradiating ultrasonic waves on the side surface portion of the semi-bell-shaped housing, that is, the housing portion including the left and right upper surfaces forming the bell shape of the housing. An animal fixing base characterized in that the part has a fixing plate that can move according to the size of the animal.

  When performing an ultrasonic irradiation experiment on a small animal such as a rat or a mouse, the animal fixing table of the present invention can irradiate the target site with ultrasonic waves without anesthesia. It has a structure that also takes into account the growth stage, so that ultrasonic irradiation stimulation can be reliably applied to the target site. According to the present invention, it is possible to perform an ultrasonic irradiation experiment on a small animal such as a rat or a mouse by fixing the animal without anesthesia or non-invasiveness, and the influence of more accurate ultrasonic irradiation stimulation. Data on is obtained.

Hereinafter, the present invention will be described in detail.
The animal fixing base of the present invention has a semi-bell-shaped housing with a closed end, an opening through which a mouse or a rat can enter on the other end, and a housing on the opening end side. And an animal fixing base having a fixing plate that can be installed in multiple stages to restrict its movement in accordance with the size of the animal, characterized in that the tip portion of the semi-bell-shaped housing is shielded from light. To do.

  As shown in FIG. 1, it is comprised with the resin material of the shape of a semi-bell, the front-end | tip part obstruct | occludes, and the other end part has the semicircle-shaped entrance part into which an animal invades. Usually, a commercially available fixture is made of a transparent material made of acrylic. At least the tip of the animal fixing base of the present invention is made of a light-shielding material, and the animal can be fixed without stress by utilizing the property that rats, mice, etc. prefer darkness. Therefore, it is desirable to secure a light-shielding portion on the side surface of the tip portion that at least hides the position of the animal's eyes.

  In order to fix the animal to the distal end side, the casing on the end side of the opening has a fixing plate that can be installed in multiple stages to limit its movement according to the size of the animal. Normally, several stages of cuts are made on the side of the housing, and a plate for fixing the tail of the animal is fitted according to the size of the animal to be used. An incision can be made in the center of the fixed plate to produce a tail.

  Since rodents such as rats and mice have good body flexibility, the body can be rotated 180 degrees only with some room in the housing of the fixation device. Therefore, it is preferable to provide a housing having a size suitable for the animal to be used. However, it is impossible to handle animals of all sizes. By adopting a method of sticking a sponge material having a light shielding property on the inner wall of the housing with a different thickness as a light shielding method, it is possible to assist in fixing the animal.

  Using the animal fixing table of the present invention, the animal is fixed without anesthesia, and ultrasonic irradiation stimulation is performed. Therefore, a hole is made in the side of the casing around the tissue site to be irradiated, and the size of the ultrasonic transducer is the same. A structure in which a plate with holes is attached is used. Such a fixed plate can be moved back and forth, and can be adapted to change of the irradiation position according to the growth of the animal. As a method of fixing the movable fixing plate, a slide-type fixing tool can be provided, or a fixing tool using a rubber band or the like can be used.

  An animal fixation platform was developed on the assumption that a small animal (rat) is used to stimulate the irradiation of ultrasound to the kidney. As shown in FIGS. 1 and 2, a part of a commonly used acrylic small animal fixation table (per contact located in the rat kidney) is cut into a rectangular shape, and further an ultrasonic transducer (this time 30 mm) A plate having a hole equivalent to the size of (about) was prepared, and both were fixed with a rubber band or the like (FIG. 3). The rat was placed in a fixed base and the tail was fixed, and then the gel was applied to the ultrasonic vibrator and placed (FIG. 4). In order to prevent movement of the cord of the vibrator, a cut was made in the plate that fixed the rat's tail, and the cord was sandwiched. With these fixed bases, ultrasonic irradiation stimulation for up to 120 minutes was possible without anesthesia.

It is drawing of the fixed stand for small animals. It is a general-view figure of a fixed stand. It is a general-view figure of the fixed base at the time of putting a rat. It is a general-view figure of a fixed stand at the time of setting an ultrasonic vibrator.

Claims (4)

  It has a semi-bell-shaped housing with a closed tip, an opening through which the mouse or rat can enter on the other end, and the housing on the end of the opening matches the size of the animal. An animal fixing table having a fixing plate that can be installed in multiple stages to restrict the movement of the lever, wherein the tip of the semi-bell-shaped housing is shielded from light.   2. The animal fixing base according to claim 1, wherein the light shielding portion is made of a light shielding sponge material stretched on the inner wall of the housing.   The animal fixing base according to claim 1 or 2, wherein the light-shielding part is a tip part including at least the rear of the position of the eye of the animal.   A side part of the semi-bell-shaped casing is provided with a fixing part of an ultrasonic transducer for irradiating ultrasonic waves, and the fixing part has a fixing plate that can move according to the size of the animal. Item 4. The animal fixing table according to any one of Items 1 to 3.

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