JP2005213388A - Fatty acid solubilizing agent and detergent composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a fatty acid solubilizing agent and a detergent composition containing the same exhibiting high detergency to sebum stains accumulated on textile fibers. <P>SOLUTION: The fatty acid solubilizing agent comprises as active ingredient a protein selected from fatty acid-binding protein, fatty acid synthase, triacyl glycerol synthase, fatty acid acyl CoA synthase, fatty acid transport protein, apolipoprotein, albumin, phospholipase and diacyl glycerol kinase. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a fatty acid solubilizing agent and a detergent composition having an excellent solubilizing action on a fixed fatty acid-containing composite soil.

  Textile fabric products are contaminated with various soils derived from food and living environment, including sebum and protein-derived soils. Various cleaning compositions have been developed for the purpose of removing these stains.

  Fatty acids contained in sebum combine with proteins and other lipids to form fatty acid complex stains. If such dirt accumulates on the fiber, it will be difficult to remove the dirt because it forms calcium and salt in tap water, and it will be difficult to remove, and it may cause odor and yellowing. It has been demanded.

Until now, as a method for cleaning sebum soil, a method for improving the cleaning power by adding an enzyme in the cleaning agent has been proposed. As such a proposal, a presoaked detergent composition in which a specific nonionic surfactant and a lipolytic enzyme are combined (see Patent Document 1: JP 2001-64696 A), a protease, and a cellulase enzyme are combined. A cleaning composition (Patent Document 2: JP 2002-265997 A) and the like have been proposed. However, even when such a composition is used, fatty acid composite stains remain, and the removal of the stains is not sufficient. For this reason, there has been a strong demand for removal of fatty acid composite stains remaining on the fibers.
JP 2001-64696 A JP 2002-265997 A JP 2002-51768 A

  This invention is made | formed in view of the said situation, and it aims at providing the fatty-acid solubilizer and detergent composition which show high detergency with respect to the sebum dirt accumulate | stored in a fiber.

  As a result of intensive studies to achieve the above object, the present inventor has found that a specific protein has an excellent fatty acid solubilizing action, and further, when used simultaneously with an enzyme, the residue of fatty acid complex soil on fibers can be reduced. It has been found and the present invention has been made.

  The present applicant has previously proposed a detergent composition containing a chimeric enzyme in which a protein having affinity for lipid and an enzyme is combined (see Patent Document 3: Japanese Patent Application Laid-Open No. 2002-51768). However, it is a new finding of the present applicant that the protein itself having affinity has an excellent fatty acid solubilizing ability.

Therefore, the present invention
[1]. A fatty acid solubilizer comprising a protein selected from fatty acid binding protein, fatty acid synthase, triacylglycerol synthase, fatty acylCoA synthase, fatty acid transport protein, apolipoprotein, albumin, phospholipase and diacylglycerol kinase as an active ingredient,
[2]. The fatty acid solubilizer according to [1], wherein the protein is a fatty acid binding protein;
[3]. A detergent composition comprising the fatty acid solubilizer according to [1] or [2],
[4]. Furthermore, the detergent composition according to [3], comprising an enzyme,
[5]. The detergent composition according to [3] or [4], wherein the enzyme is one or more selected from a proteolytic enzyme, a lipolytic enzyme, and a carbohydrase.

  According to the present invention, it is possible to obtain a fatty acid solubilizer and a detergent composition that exhibit high detergency against sebum soil accumulated in fibers.

Hereinafter, the present invention will be described in more detail.
The fatty acid solubilizing agent of the present invention is a protein selected from fatty acid binding protein, fatty acid synthase, triacylglycerol synthase, fatty acyl acyl CoA synthase, fatty acid transport protein, apolipoprotein, albumin, phospholipase and diacylglycerol kinase. One type can be used alone, or two or more types can be used in appropriate combination. Of these, fatty acid binding proteins are preferred.

  These proteins dissolve fatty acids attached to clothes and fibers and insolubilized, and have a fatty acid solubilizing ability.

  When the protein of the present invention is used in a detergent composition, the blending amount is preferably 0.001 to 50% by mass, more preferably 0.01 to 20% by mass, and particularly preferably 0.1 to 10% by mass. %. If the amount is too small, sufficient detergency may not be obtained. On the other hand, if the amount is too large, detergency corresponding to the amount may not be expected.

  When the fatty acid solubilizer of the present invention is used in a cleaning composition, other components can be blended depending on the type and dosage form. Examples of other components include enzymes and surfactants, and enzymes are preferably blended from the viewpoint of excellent detergency.

  Any enzyme can be used as long as it can be used as a detergent, and is not particularly limited. Enzymes include proteolytic enzymes, lipolytic enzymes such as lipases and esterases, saccharide-degrading enzymes such as cellulase, amylase, pectinase and pullulanase, oxidoreductases such as oxidases, laccases and peroxidases, etc. Proteins that have been modified or genetically engineered are also included. These can be used individually by 1 type or in combination of 2 or more types. As the enzyme, a proteolytic enzyme, a lipolytic enzyme, and a carbohydrase are preferable.

  As the source for obtaining the protein and enzyme of the present invention, those derived from animals, plants, and microorganisms such as molds and bacteria are suitable, and they can be used separately from these organisms. It is also possible to obtain DNA from microorganisms such as animals, plants, molds, and bacteria by a commonly used method, and use it for gene recombination production or cell-free production in vitro. is there. For example, by selectively amplifying a target DNA region by PCR or the like from a chromosome, RNA, plasmid, or cDNA library containing a fatty acid binding protein DNA sequence, or by cutting it out using an appropriate restriction enzyme It can be produced by obtaining a target gene and incorporating it into an appropriate expression plasmid to transform a host for protein production.

  0.001-30 mass% is preferable, as for the compounding quantity of the enzyme in a cleaning composition, More preferably, it is 0.01-20 mass%, Most preferably, it is 0.1-10 mass%. If the enzyme content is too small, the expected detergency may not be obtained. On the other hand, if the amount is too large, the detergency corresponding to the amount may not be expected.

  As a cleaning component such as a surfactant, one kind can be used alone, or two or more kinds can be used in appropriate combination. Anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, and the like Any of these can be used.

  The anionic surfactant is an alkyl ethoxy sulfate having a linear or branched alkyl group having an average carbon number of 10 to 20, and an average of 0.15 to 8 mol of ethylene oxide added to the molecule, an average of 10 to 10. Olefin sulfonate having 20 carbon atoms in one molecule, α-sulfo fatty acid methyl ester salt having 10 to 20 carbon atoms in one molecule, higher fatty acid salt having 8 to 20 carbon atoms in average, and other phosphoric acid Examples include ester surfactants.

  Examples of amphoteric surfactants include betaine-type amphoteric surfactants and sulfonic acid-type amphoteric surfactants.

  Nonionic surfactants include polyoxyalkylene alkyl ethers having an alkyl group having an average carbon number of 10 to 20 and an average of 1 to 20 moles of alkylene oxide added to the molecule, higher fatty acid alkanolamides, or addition of alkylene oxides thereof. Thing etc. are mentioned.

  Among these, linear alkylbenzene sulfonate and alkyl sulfate are preferable. Specific examples thereof include a linear alkylbenzene sulfonate having an alkyl group having an average carbon number of 10 to 16, an alkyl sulfate having an alkyl group having an average carbon number of 10 to 20, and the like.

  The compounding quantity of surfactant in the cleaning composition of this invention is 5-40 mass% normally, Preferably it is 15-40 mass%. If the amount of the surfactant is too small, the detergency may be insufficient. On the other hand, if the amount is too large, the detergent productivity may be deteriorated.

In addition, the following components can be used alone or in combination of two or more as necessary in the cleaning composition of the present invention.
(1) Copolymer of acrylic acid and maleic acid (2) Inorganic builder A-type zeolite, P-type zeolite, X-type zeolite, amorphous aluminosilicate, crystalline sodium silicate, sodium tripolyphosphate, sodium pyrophosphate, etc. Is mentioned.
(3) Divalent metal ion scavenger Amino acid derivative (a chelating agent having 3 to 4 —COOX groups in the molecule derived from glycine, serine, aspartic acid and glutamic acid as the amino acid skeleton, and X is hydrogen or alkali Metal salts, particularly sodium salts are preferred, specifically glycine diacetate, serine diacetate, aspartic acid diacetate, glutamic acid diacetate, iminodisuccinate, hydroxyiminodisuccinate, etc.), orthophosphoric acid Salt, pyrophosphate, tripolyphosphate, nitrile triacetate, ethylenediaminetetraacetate, citrate, isocitrate, polyacrylic acid, polyacetal carboxylate and the like.
(4) Alkali agent and inorganic salt Examples include silicates, carbonates, sesquicarbonates, sulfates, and sodium chloride.
(5) Recontamination inhibitor Polyethylene glycol, polyvinyl alcohol, polyvinyl pyrrolidone, carboxymethyl cellulose and the like can be mentioned.
(6) Bleaching agent, bleach activator, fluorescent agent, etc. Examples of the bleaching agent include sodium percarbonate, sodium perborate, sodium sulfate, and hydrogen peroxide adduct of sodium chloride. Examples of the bleach activator include those described in JP-A-8-157876, and examples of the whitening agent include commercially available dyes, fragrances, and bluing agents.
(7) Carryover component The impurities in the raw material can be blended as long as they do not adversely affect the performance. Acrylic acid or maleic acid monomer contained in sodium methylsulfate, sodium methanesulfonate, α-sulfo fatty acid salt, acrylic acid and maleic acid copolymer (salt) contained in α-sulfo fatty acid methyl ester salt or Examples thereof include higher alcohols and polyethylene glycols contained in the salts, ethylene oxide adducts of higher alcohols.

  The site where a large amount of sebum stains such as elbows, swords, and armpits on clothes adhere to the soil, the accumulated sebum stains form calcium and salt in the tap water and become difficult to remove. Further, these sebum stains are very hydrophobic and involve water and water-insoluble stains, including proteins and other stain components. On the other hand, by using the fatty acid solubilizing agent of the present invention, it is possible to effectively remove the sebum dirt that is easily fixed by dissolving the fatty acid in the sebum dirt. Furthermore, the combined use of enzymes can effectively improve the action of removing dirt components other than fatty acids in sebum dirt.

  EXAMPLES Hereinafter, although a preparation example, a test example, an Example, and a comparative example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to the following Example.

[Preparation Example 1]
Preparation of fatty acid binding protein (hereinafter abbreviated as FABP) The fatty acid binding protein FABP gene sequence was obtained as follows.
Human skin-derived keratinocyte mRNA was prepared using QuickPrep mRNA purification Kit (manufactured by Amersham Pharmacia).
First, with reference to GeneBank database registration number MN001444, the following primer 1 containing an EcoRI site on the N-terminal side and the following primer 2 (SEQ ID NOs: 1 and 2) containing a PstI site on the C-terminal side were prepared, and RNA PCR Kit ( AMV) Ver. RT-PCR was performed using 2.1 (Takara Shuzo Co., Ltd.).
Primer 1: SEQ ID NO: 1
gaattcatgg ccacagtca g
Primer 2: SEQ ID NO: 2
ctgcagttat tctacttttt catagatcc

  The amplified fragment was cloned into pT7Blue (manufactured by Novagen) to obtain a plasmid pT7FABP containing the FABP gene sequence. The plasmid pKKFABP was prepared by inserting the EcoRI and PstI digested fragment of this plasmid into the EcoRI and PstI sites of pKK233-3 (Amersham Pharmacia). Escherichia coli was transformed with this plasmid, and this was cultured overnight, then 1 mM IPTG was added, and further cultured for 4 hours to obtain FABP.

[Test Example 1]
<Fatty acid solubilization test method for fatty acid binding protein>
The following fatty acid solubilization test was performed using FABP obtained in Preparation Example 1. The results are shown in Table 1.

  On a 1 cm × 1 cm cotton cloth, 50 μL of 1% oleic acid (dissolved in chloroform) and 50 μL of 0.5% triolein (dissolved in chloroform) were applied and dried to be fixed to the cloth to prepare a fatty acid-fixed cloth. This fixed cloth was dipped in 400 μL of a 0.1% FABP solution and an unblended FABP solution, respectively, and kept at 37 ° C. for 30 minutes. Thereafter, the amount of liberated fatty acid released in 50 μL of the supernatant was measured with NEFA C-Test Wako (manufactured by Wako Pure Chemical Industries, Ltd.).

[Examples 1 to 7, Comparative Example 1]
A detergent composition having the composition shown in Table 2 was prepared under the conditions described in Table 3 for the enzyme and fatty acid binding protein. In Table 3, the case where the fatty acid binding protein is blended is indicated as “◯”, and the case where no fatty acid is blended is represented as “−”. The cleaning power test of the obtained cleaning composition was performed according to the following method. The results are also shown in Table 3.

<Detergency test method>
(1) Preparation method of contaminated cloth A clay mainly composed of crystalline minerals kaolinite, vermiculite and the like dried at 200 ° C. for 30 hours was used as inorganic soil. While adding 10 g of the above-mentioned inorganic dirt to 900 mL of a liquid obtained by mixing 10% by weight fruit juice, 1% by weight fresh powder, and 10% by weight hemoglobin with a mixer and dispersing with polytron, 30 g of oleic acid and 30 g of triolein To prepare a dirt bath. After immersing 10 cm × 20 cm cotton cloth No. 20 in this dirt bath, water was squeezed with two rubber rolls to uniformize the amount of dirt adhered. This cloth was dried at 105 ° C. for 30 minutes, washed with 300 ppm LAS (sodium linear alkylbenzene sulfonate) as a detergent solution for 10 minutes, rinsed for 3 minutes and dehydrated for 1 minute, followed by natural drying. The cloth which repeated this operation 5 times was cut | judged to 5 cm x 5 cm, and the contaminated cloth for evaluation was produced.
(2) Detergency test S. Using a Terg-0-meter of Testing (USA), the above 10 contaminated fabrics and knitted fabrics were added to adjust the bath ratio to 30 times, and washed with a detergent composition at 30 ° C., 120 rpm for 10 minutes. Thereafter, after rinsing and drying, the reflectivity was measured to determine the cleaning power. In this case, 900 mL of cleaning solution having a cleaning agent concentration of 0.066% was used, and rinsing was performed with 900 mL of water for 3 minutes. The water used had a hardness of 3 ° DH and a chlorine concentration of 1 ppm.
(3) Evaluation method The detergency (%) was determined by the Kubelka-Munk formula expressed by the following formula 1. In addition, evaluation of the detergency was performed by an average value of 10 test cloths. The standard white cloth is a cotton cloth No. 20 washed with the composition shown in Table 1 (without enzyme and fatty acid binding protein).

（式中、Ｒは日本電色（株）製の色彩計Σ−９０００を用いて測定した反射率である。）

(In the formula, R is a reflectance measured using a colorimeter Σ-9000 manufactured by Nippon Denshoku Co., Ltd.)

The meaning and details of the abbreviations in Table 1 are as follows, and the mass% values in the table are values in terms of pure content. In addition, EOp shows the average added mole number of ethylene oxide, and POp shows the average added mole number of propylene oxide.
LAS: C ₁₀ ~C ₁₄ linear alkylbenzene sulfonate, sodium alpha-SF: alpha-sulfo fatty acid (C ₁₄ ~C ₁₆₎ methyl ester Sodium AOS: C ₁₄ ~C ₁₈ α- olefin sulfonate sodium soap: C ₁₂ -C ₁₈ sodium palm oil-derived fatty AE: C ₁₂ alcohol ethoxylate (EOp = 12)
AEP: C ₁₃ alcohol EO, PO adducts (EOp = 12, POp = 3 )
Copolymer: Sodium salt of a copolymer having a molar ratio of acrylic acid and maleic acid of 60:40 and an average molecular weight of 50,000, Germany; manufactured by BASF, trade name Socalan CP7
Zeolite: Type 4A zeolite (average particle size 1.2 μm)
Fluorescent agent: 4.4-bis (sulfostyryl) biphenyl disodium / 4.4-bis {(4-anilino-6-monophorino-1,3,5-triazin-2-yl) amino} stilpene-2,2- Disulfone disodium = 1/1 mixture (trade name Chino Pearl CBS / trade name Chino Pearl AMS)

Claims (5)

  A fatty acid solubilizing agent comprising a protein selected from fatty acid binding protein, fatty acid synthase, triacylglycerol synthase, fatty acid acyl CoA synthase, fatty acid transport protein, apolipoprotein, albumin, phospholipase and diacylglycerol kinase as an active ingredient.   The fatty acid solubilizer according to claim 1, wherein the protein is a fatty acid binding protein.   A cleaning composition comprising the fatty acid solubilizer according to claim 1.   Furthermore, the cleaning composition of Claim 3 formed by containing an enzyme. The detergent composition according to claim 3 or 4, wherein the enzyme is one or more selected from a proteolytic enzyme, a lipolytic enzyme, and a carbohydrase.