JP2005176619A5 - - Google Patents

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JP2005176619A5
JP2005176619A5 JP2003418160A JP2003418160A JP2005176619A5 JP 2005176619 A5 JP2005176619 A5 JP 2005176619A5 JP 2003418160 A JP2003418160 A JP 2003418160A JP 2003418160 A JP2003418160 A JP 2003418160A JP 2005176619 A5 JP2005176619 A5 JP 2005176619A5
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JP
Japan
Prior art keywords
rapeseed
seq
oligonucleotide
resistant
base sequence
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JP2003418160A
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Japanese (ja)
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JP4469992B2 (en
JP2005176619A (en
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Priority to JP2003418160A priority Critical patent/JP4469992B2/en
Priority claimed from JP2003418160A external-priority patent/JP4469992B2/en
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Publication of JP2005176619A5 publication Critical patent/JP2005176619A5/ja
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Publication of JP4469992B2 publication Critical patent/JP4469992B2/en
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Claims (6)

配列番号３で示される塩基配列を含むポリヌクレオチドからなる根こぶ病抵抗性ナタネ類の選抜用マーカー。   A marker for selection of root-knot-resistant rapeseed consisting of a polynucleotide comprising the base sequence represented by SEQ ID NO: 3. 配列番号３で示される塩基配列を含むポリヌクレオチドに特異的にハイブリダイズし、該配列を増幅するためのオリゴヌクレオチド。   An oligonucleotide for specifically hybridizing to a polynucleotide comprising the base sequence represented by SEQ ID NO: 3 and amplifying the sequence. ナタネ類より抽出したRNA又はDNAから、請求項１記載のマーカーを検出することを特徴とする、根こぶ病抵抗性ナタネ類の選抜方法。   A method for selecting root-knot-resistant rapeseed, comprising detecting the marker according to claim 1 from RNA or DNA extracted from the rapeseed. 請求項２記載のオリゴヌクレオチドを含む、請求項３記載の方法。   4. The method of claim 3, comprising the oligonucleotide of claim 2. 以下の工程を含む、根こぶ病抵抗性ナタネ類の選抜方法：
１）ナタネ類より抽出したRNA又はDNAを鋳型として、請求項２記載のオリゴヌクレオチドを用いて核酸増幅反応を行い；
２）得られた核酸増幅産物から、配列番号３で示される塩基配列を含む増幅産物を検出することにより、根こぶ病抵抗性ナタネ類を選抜する。 Method for selecting clubroot resistant rape, including the following steps:
1) Performing a nucleic acid amplification reaction using the oligonucleotide according to claim 2 using RNA or DNA extracted from rapeseed as a template;
2) A clubroot resistant rapeseed is selected by detecting an amplification product containing the base sequence represented by SEQ ID NO: 3 from the obtained nucleic acid amplification product. 検出が電気泳動分析を利用して行われる、請求項３〜５のいずれか１項に記載の方法。   The method according to claim 3, wherein the detection is performed using electrophoretic analysis.
JP2003418160A 2003-12-16 2003-12-16 Selection marker for clubroot resistant rapeseed Expired - Fee Related JP4469992B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2003418160A JP4469992B2 (en) 2003-12-16 2003-12-16 Selection marker for clubroot resistant rapeseed

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2003418160A JP4469992B2 (en) 2003-12-16 2003-12-16 Selection marker for clubroot resistant rapeseed

Publications (3)

Publication Number Publication Date
JP2005176619A JP2005176619A (en) 2005-07-07
JP2005176619A5 true JP2005176619A5 (en) 2007-01-25
JP4469992B2 JP4469992B2 (en) 2010-06-02

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JP2003418160A Expired - Fee Related JP4469992B2 (en) 2003-12-16 2003-12-16 Selection marker for clubroot resistant rapeseed

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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101988118B (en) * 2009-08-07 2012-11-21 中国农业科学院油料作物研究所 Rape oil content character major gene resistance bit and application
JP5721209B2 (en) 2010-09-22 2015-05-20 国立研究開発法人農業・食品産業技術総合研究機構 Production method of clubroot resistant plant
US9516823B2 (en) * 2010-11-29 2016-12-13 Bejo Zaden B.V. Plasmodiophora brassicae-resistant Brassica plant, seeds and plant parts thereof and methods for obtaining the same
EP3447151A1 (en) * 2011-09-27 2019-02-27 Dow AgroSciences LLC Ho/ll canola with resistance to clubroot disease
CN103667484B (en) * 2013-12-09 2015-02-18 中国农业科学院油料作物研究所 Oil content character main gene locus of rape 6F313 and applications thereof
CN108588264B (en) * 2018-06-08 2021-05-28 西南大学 Molecular marker and primer for identifying cabbage clubroot disease resistance and application of molecular marker and primer
KR102415534B1 (en) 2021-12-30 2022-07-01 충남대학교 산학협력단 Molecular marker based on high-resolution genetic map for discriminating clubroot disease-resistant or sensitive cabbage cultivar and uses thereof

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