JP2005164523A - Sample suction and dispensing apparatus, and sample-producing apparatus with the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a sample suction and dispensing apparatus which has a simplified structure, having a suction section and a dispensing section. <P>SOLUTION: A sucking and dispensing mechanism (sample suction and dispensing apparatus) 1 of a blood smear-producing apparatus 100 is provided with a piercer (a suction needle) 11 for sucking blood; a dispensing pipette 12 for dispensing the sucked blood; a support comprising a first support member 13 and a second support member 14 for supporting the piercer 11 and the dispensing pipette 12; and a drive section comprising a slide screw 22, a coupling 24 and a motor 26 for moving the support in the vertical directions. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a sample suction / dispensing device and a specimen preparation device including the same, and more particularly to a sample suction / dispensing device including a suction portion and a dispensing portion and a specimen preparation device including the same.

  2. Description of the Related Art Conventionally, a specimen preparation device including a sample suction / dispensing device including a suction portion and a dispensing portion is known (see, for example, Patent Document 1).

Patent Document 1 discloses a blood smear preparation apparatus that automatically performs the process from smearing blood on a slide glass to a staining step. In the blood smear preparation apparatus disclosed in Patent Document 1, a suction part made of a piercer (aspiration needle) for sucking blood from a sealed specimen container, and a dispensing part made of a pipette for dropping blood on a slide glass; A suction / dispensing mechanism portion having the above is provided.
JP 2001-272317 A

  In the suction dispensing mechanism portion having the suction portion composed of a piercer (suction needle) and the dispensing portion composed of a pipette as disclosed in Patent Document 1, conventionally, the suction portion composed of the piercer (suction needle) is driven. Since the mechanism and the mechanism that drives the pipetting part are separately provided, the structure of the suction and dispensing mechanism part is complicated.

  The present invention has been made to solve the above-described problems, and a sample suction / dispensing device capable of simplifying a structure having a suction portion and a dispensing portion, and a specimen preparation including the same. An object is to provide an apparatus.

Means for Solving the Problems and Effects of the Invention

  A sample suction / dispensing device according to a first aspect of the present invention supports a suction part for sucking a sample, a dispensing part for dispensing a sucked sample, and a suction part and a dispensing part. A support unit and a drive unit for moving the support unit in the vertical direction are provided.

  In the sample suction / dispensing device according to the first aspect, as described above, by providing the support portion that supports the suction portion and the dispensing portion, and the drive portion for moving the support portion in the vertical direction, Since the suction unit and the dispensing unit can be driven by a single drive unit, the structure can be simplified compared to the case where a mechanism for driving the suction unit and a mechanism for driving the dispensing unit are provided separately. Can do. Moreover, since the length of the tube to be connected can be shortened and the moving distance of the sample is shortened, for example, damage to blood cells in the sample can be reduced.

  In the sample suction / dispensing device according to the first aspect, preferably, the suction part includes a suction needle, and further includes a suction needle cleaning member provided below the suction needle and cleaning the suction needle. If comprised in this way, the sample adhering to the outer surface of the suction needle can be easily washed away by washing.

  In the sample suction / dispensing device including the suction needle cleaning member, preferably, the suction needle cleaning member has a guide hole for guiding the suction needle. If comprised in this way, it can suppress that a suction needle bends by a guide hole, when an elongate suction needle is stabbed in the sealing stopper of a sample container. Further, since the guide hole is provided in the suction needle cleaning member, it is not necessary to separately provide a member for guiding the suction needle, so that the structure can be simplified correspondingly.

  In this case, the suction needle cleaning member is preferably installed so as to be movable in the vertical direction, and moves up and down as the suction needle moves up and down while guiding the tip of the suction needle through the guide hole. If comprised in this way, since the front-end | tip part of a suction needle can always be set to the state guided by the guide hole, it is not necessary to insert the front-end | tip part of a suction needle into a guide hole with little clearance for every suction process. Thereby, when inserting the front-end | tip part of a suction needle in a guide hole with little clearance, it can prevent that the problem that the front-end | tip part of a suction needle cannot be inserted appropriately in a guide hole generate | occur | produces.

  In the sample suction / dispensing device including the suction needle cleaning member, preferably, the suction needle cleaning member has a pressing portion that presses the container in which the sample is accommodated. According to this structure, when the sealing stopper of the specimen container is pierced by the suction needle, the sealing stopper of the specimen container can be stably supported by the holding portion, so that the sealing stopper of the specimen container can be easily supported by the suction needle. You can break through. Further, by providing the pressing portion on the suction needle cleaning member, there is no need to separately provide a member for pressing the container in which the sample is accommodated, so that the structure can be simplified correspondingly.

  In the sample aspirating / dispensing device according to the first aspect, preferably, the aspirating portion is provided in the vicinity of the tip portion having a triangular pyramid shape capable of breaking through the sealing plug of the specimen container in which the sample is accommodated. And a suction needle having a suction port made of a horizontal hole. If comprised in this way, it is hard to bend | curve compared with the suction needle of the conventional single blade, and since the suction force when the suction needle pierces the sealing plug can be positioned on the center line of the suction needle by the triangular pyramid-shaped tip portion. The tip of the suction needle can easily break through the sealing stopper of the sample container. Further, the sample can be easily sucked by the lateral hole of the suction needle.

  In the sample suction / dispensing device according to the first aspect, preferably, the dispensing unit is attached to the support unit so as to be movable in the front-rear direction. If comprised in this way, since this movement of the front-back direction and the movement of the up-and-down direction of a support part can move a dispensing part to the front, it can be dropped and dispensed, The front of a dispensing part Even when the slide glass is disposed below, the slide glass can be easily dispensed.

  In the sample suction / dispensing device according to the first aspect, preferably, the dispensing unit is attached to the support unit so as to be movable in the vertical direction, and is biased to bias the dispensing unit downward. Means are further provided. If constituted in this way, even when the dispensing part descends and collides with the slide glass at the time of dispensing, the dispensing part can escape upward against the urging force of the urging means. Can be effectively prevented from being damaged.

  In the sample suction / dispensing device according to the first aspect, preferably, the dispensing unit includes a pipette having a notch groove at the tip. If comprised in this way, even if a pipette contacts a slide glass by the notch groove | channel and the pipette contacts a slide glass by the variation in the thickness of a pipette, the exact quantity can be dispensed.

  In the sample suction / dispensing device according to the first aspect, the dispensing unit preferably includes a pipette, and further includes a pipette cleaning member for cleaning the pipette. If comprised in this way, since the pipette washing | cleaning member can wash | clean the outer peripheral part of a pipette for every dispensing operation | movement, the specimen sample before adhering to the outer peripheral part of a pipette will be at the time of dispensing of a specimen sample after that. It can be prevented from being mixed with a later specimen sample. Thereby, a high quality sample can be produced.

  In the sample aspirating / dispensing device according to the first aspect, preferably, the syringe pump connected to the dispensing unit and the aspirating unit and the support unit are disposed between the aspirating unit and the syringe pump, The first valve for opening and closing the flow path between the suction part and the syringe pump, and attached to the support part and disposed between the dispensing part and the syringe pump, and between the dispensing part and the syringe pump And a second valve for opening and closing the flow path therebetween. If comprised in this way, the 1st valve and 2nd valve for switching a flow path can be easily moved up and down with a suction part and a dispensing part by moving a support part up and down.

  In the sample suction / dispensing device according to the first aspect, preferably, the sample suction / dispensing device further includes a syringe pump connected to the dispensing unit and the suction unit, and the syringe pump includes: a pump main unit having a piston insertion hole; A piston that is movably inserted into the piston insertion hole, a seal member that is disposed in the piston insertion hole of the pump body, and seals between the piston insertion hole and the piston of the pump body, and sandwiches the seal member And a guide member that is disposed in the piston insertion hole of the pump body and guides the piston. If comprised in this way, since a piston is guided by a guide member, it can suppress that a piston inclines. As a result, it is possible to prevent the seal member from being worn due to the piston moving up and down in an inclined state, so that the reagent such as physiological saline leaks from the piston insertion hole due to wear of the seal member. Can be prevented.

  A specimen preparation device according to a second aspect of the present invention includes a sample suction / dispensing device having each of the above-described configurations. If comprised in this way, the sample preparation apparatus which has a sample suction dispensing apparatus with the simplified structure can be provided.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

  FIG. 1 is a perspective view showing the entire configuration of a blood smear preparation apparatus and a transport apparatus according to an embodiment of the present invention, and FIG. 2 shows the inside of the blood smear preparation apparatus according to the embodiment shown in FIG. It is the top view which showed the structure. 3 and 4 are perspective views showing a cassette and a slide glass used in the blood smear preparation apparatus shown in FIG. 5 to 17 are views showing the detailed structure of the suction dispensing mechanism portion of the blood smear preparation apparatus according to the embodiment shown in FIG. First, with reference to FIGS. 1-4, the whole structure of the blood smear sample preparation apparatus and conveyance apparatus by this embodiment is demonstrated. In this embodiment, the sample preparation device of the present invention is applied to a blood smear preparation device, and the sample suction / dispensing device of the present invention is applied to the suction dispensing mechanism section of the blood smear preparation device. explain.

  First, in the present embodiment, as shown in FIG. 1, a transport apparatus 200 is installed on the front surface of the blood smear preparation apparatus 100. The transport device 200 is provided for automatically transporting the sample rack 150 storing the test tube 151 storing blood (sample) to the blood smear preparing device 100. The blood smear preparation apparatus 100 includes a display operation unit 101 including a touch panel, a start switch 102, a power switch 103, and a cover 104. The blood smear preparing apparatus 100 is provided with a hand member 160 for transporting the test tube 151 containing blood from the transport apparatus 200 side to the blood smear preparing apparatus 100 side. A rubber stopper 151a is attached to the test tube 151 containing blood. The test tube 151 is an example of the “sample container” in the present invention, and the rubber plug 151a is an example of the “sealing plug” in the present invention.

  Further, as shown in FIG. 2, the blood smear preparation apparatus 100 includes a suction dispensing mechanism unit 1, a smear unit 2, a resin cassette 3, a cassette carry-in unit 4, a cassette transport unit 5, and a slide. A glass insertion portion 6, a staining portion 7, and a storage portion 8 are provided. The suction dispensing mechanism unit 1 is an example of the “suction dispensing device” in the present invention. The suction dispensing mechanism unit 1 has a function of sucking blood from a test tube 151 transported to the blood smear preparation apparatus 100 side by a hand member 160 (see FIG. 1) and dropping the sucked blood onto the slide glass 10. Have The smearing unit 2 is provided to supply the slide glass 10 to the dispensing / smearing position 90, to smear and dry the blood dropped on the slide glass 10, and to print on the slide glass 10. ing. The smearing section 2 includes a slide glass supply section 2a, a slide glass storage section 2b, a pulling glass 2c, feed belts 2d and 2e, a fan 2f, moving pieces 2g and 2i, and a printing section 2h. It has been.

  The slide glass supply unit 2a has a function of supplying the slide glass 10 stored in the two slide glass storage units 2b onto the feed belt 2e using a take-out mechanism and a feed belt 2d (not shown). The feed belt 2e is configured to convey the slide glass 10 to the dispensing / smearing position 90 and the drying positions 91a and 91b. The pulling glass 2c is movable to a position where it abuts on the slide glass 10 so that blood dispensed on the slide glass 10 can be smeared at the dispensing / smearing position 90, and the longitudinal direction of the slide glass 10 It is configured to be movable. The fan 2f is provided to dry smeared blood on the slide glass 10 conveyed to the drying positions 91a and 91b. The moving piece 2g is provided to move the slide glass 10 from the drying position 91b to the printing unit 2h. The printing unit 2h is a printer, and is provided on the frosted portion 10a of the slide glass 10 for printing the sample number, date, reception number, name, and the like. The moving piece 2 i is provided to move the slide glass 10 after printing to the slide glass insertion portion 6 side and insert it into the cassette 3.

  Further, the resin cassette 3 is configured so as to be able to accommodate the smeared slide glass 10 and the liquid (staining liquid) used in the staining step. Specifically, as shown in FIGS. 3 and 4, the cassette 3 includes a slide glass storage hole 3a, a staining liquid suction / dispensing hole 3b, partition portions 3c and 3d, a slide glass support portion 3e, and a magnet. It includes two metal adsorbing members 3f made of metal that can be adsorbed to each other, a conveyor belt engaging portion 3g, and a side surface portion 3h. The slide glass storage hole 3a and the staining liquid suction / dispensing hole 3b are connected inside. Moreover, as shown in FIG. 2, the cassette carrying-in part 4 is provided in order to carry in the cassette 3, and contains the infeed belt 4a. The cassette carry-in unit 4 is provided with a sensor 4b for detecting that the number of cassettes 3 at the standby position 92 of the cassette carry-in unit 4 is equal to or less than a predetermined number (for example, 10).

  The cassette carrying unit 5 is provided for carrying the cassette 3 carried in from the cassette carrying-in unit 4 to the slide glass inserting unit 6 and the staining unit 7. The cassette transport unit 5 includes a cassette transport member 5a that can move in the horizontal direction and a drive belt 5b for moving the cassette transport member 5a in the horizontal direction. The cassette conveying member 5a is attached to the drive belt 5b so as to be moved integrally with the drive belt 5b. The drive belt 5b is driven by a drive motor (not shown). Further, the cassette carrying member 5a is provided with a carrying claw 5c (see FIG. 2) that carries the cassette 3 while pushing the side surface portion 3h (see FIG. 3).

  The slide glass insertion portion 6 is provided for storing the slide glass 10 that has been smeared and printed in the slide glass storage hole 10 a of the cassette 3. The slide glass insertion portion 6 includes a cassette direction moving member 6 a for placing the cassette 3 in the horizontal direction so that the slide glass 10 can be inserted, and a slide glass on the cassette 3 positioned in the slide glass insertion portion 6. And a sensor 6b for detecting whether or not 10 is accommodated. The cassette direction moving member 6a is configured to be movable between a horizontal position and a vertical position by rotating in a predetermined direction.

  The staining unit 7 is provided for staining the smeared slide glass 10 by supplying the staining liquid to the staining liquid suction / dispensing hole 3b of the cassette 3 conveyed by the cassette conveying member 5a. The dyeing unit 7 includes a feeding member 7a for feeding the cassette 3 conveyed by the cassette conveying member 5a to the staining unit 7, a conveying belt 7b for conveying the cassette 3 fed from the feeding member 7a, and the cassette 3 The first to fifth suction / discharge sections 7c to 7g for supplying and discharging the staining liquid, the fan 7h for drying the stained slide glass 10, and the storage section for removing the cassette 3 from the transport belt 7b. 8 includes a delivery mechanism 7i for delivering the toner to the conveyor belt 8a side, and a fan 7j for drying the slide glass 10 in the second suction / discharge unit 7d.

  The storage unit 8 is provided for storing the cassette 3 in which the slide glass 10 stained by the staining unit 7 is stored. The storage unit 8 includes a feeding member 8a for feeding the cassette 3 fed from the conveying belt 7b of the dyeing unit 7 by the feeding mechanism unit 7i to the conveying belt 8b of the storing unit 8, and a cassette fed from the feeding member 8a. 3 is provided with a conveyor belt 8b.

  Here, with reference to FIGS. 5-17, the detailed structure of the suction dispensing mechanism part 1 by this embodiment is demonstrated. In the suction / dispensing mechanism unit 1 according to the present embodiment, as shown in FIG. 5, a stainless steel piercer (suction needle) 11 for sucking blood is supported by a resin piercer support portion 45. The piercer support portion 45 is attached to the first support member 13 made of a thick metal material. The piercer 11 is an example of the “suction unit” in the present invention. A second support member 14 made of sheet metal is integrally fixed to the first support member 13. The first support member 13 and the second support member 14 are examples of the “support portion” in the present invention. In addition, a stainless steel pipette 12 for dropping (dispensing) blood on the slide glass 10 includes a pipette support 31, a mounting plate 32, a fixing member 39, a slide body 34, and a slide rail 33. 2 It is attached to the support member 14. The dispensing pipette 12 is an example of the “dispensing part” and “pipette” in the present invention. As described above, the piercer (suction needle) 11 and the dispensing pipette 12 are supported by the support portion including the first support member 13 and the second support member 14.

  Further, as shown in FIG. 5, valves 15 and 16 are attached to the second support member 14. The valve 15 is provided to open and close the flow path between the piercer (suction needle) 11 and the syringe pump 70, and the valve 16 opens and closes the flow path between the dispensing pipette 12 and the syringe pump 71. Is provided to do. The valve 15 is an example of the “first valve” in the present invention, and the valve 16 is an example of the “second valve” in the present invention. Rubber tubes 17 and 18 are attached to the valves 15 and 16, respectively. The valve 15 is connected to the piercer 11 via a rubber tube 17 and a piercer tube 19 made of a Teflon (registered trademark) tube. The valve 15 is connected to the syringe pump 70 via the rubber tube 17, the relay member 30, and the syringe tube 21 made of a Teflon (registered trademark) tube, and the rubber tube 17 and the relay member 30. And a rubber tube 18 to be connected to the valve 16. The valve 16 is connected to the dispensing pipette 12 via a dispensing pipette tube 20 made of a rubber tube 18 and a Teflon (registered trademark) tube. The valve 16 is connected to the syringe pump 70 via the rubber tube 18, the relay member 30 and the syringe tube 21. Thus, a fluid circuit as shown in FIG. 8 is configured.

  The first support member 13 is configured to be movable in the vertical direction by a slide screw 22. The slide screw 22 is rotatably supported by a lower holder 23 and an upper holder (not shown) installed at the lower portion of the shaft portion 22a. Further, a shaft portion 22 a is integrally provided on the upper part of the slide screw 22, and this shaft portion 22 a is connected to the shaft of the motor 26 via the coupling 24. The motor 26 is attached to the frame 80 by the motor attachment member 25. The slide screw 22, the coupling 24, and the motor 26 are examples of the “drive unit” in the present invention. The frame 80 is attached with a linear guide composed of a slide rail 27 and a slide body 28a. The first support member 13 is fixed to the slide body 28a. Accordingly, the first support member 13 and the second support member 14 integrally fixed to the first support member 13 are movable in the vertical direction along the slide rail 27. The second support member 14 is provided with a detection piece 14a for detecting the upper end position, and the light transmission type sensor 29 for detecting that the detection piece 14a has reached the upper end position is provided on the frame 80. is set up.

  Further, as shown in FIG. 9, the piercer (suction needle) 11 according to the present embodiment has a sharp triangular pyramid-shaped tip portion 11a that tapers toward the apex A, a suction channel 11b, and the vicinity of the tip portion 11a. And a suction port 11c composed of a horizontal hole connected to the suction flow path 11b. The vertex A is located on the center axis of the piercer 11 as shown in FIG. Thereby, since the force at the time of descent | fall concentrates on the vertex A, the rubber stopper 151a of the test tube 151 can be easily pierced by the triangular pyramid tip portion 11a.

  Further, as shown in FIG. 5, the suction dispensing mechanism unit 1 is provided with a piercer cleaning member 61 for cleaning the piercer (suction needle) 11. The piercer cleaning member 61 is made of polyacetal resin and is attached to a metal attachment member 62. The attachment member 62 is attached to the slide main body 28 b attached to the slide rail 27 and is connected to the air cylinder 63. The air cylinder 63 is attached to the frame 80 by an attachment plate 64. The air cylinder 63 moves the piercer cleaning member 61 in the vertical direction along the slide rail 27. Also, below the piercer cleaning member 61, a test tube support portion 65 is provided that supports the lower end portion of the test tube 151 in which blood is stored during suction by the piercer 11. The test tube support portion 65 is attached to the frame 80 by an attachment plate 66.

  Here, the detailed structure of the piercer cleaning member 61 according to the present embodiment will be described with reference to FIGS. 5 and 11 to 13. As shown in FIGS. 12 and 13, the piercer cleaning member 61 made of polyacetal resin is chamfered with four pressing portions 61 a for pressing the upper surface of the rubber stopper 151 a of the test tube 151 during suction by the piercer 11. An opening 61b, a guide hole 61c for guiding the piercer 11, a cleaning liquid injection hole 61d, and a cleaning liquid discharge hole 61e are provided. Further, as shown in FIG. 12, the four pressing portions 61a protrude from the bottom surface of the piercer cleaning member 61 and are arranged to extend radially at intervals of 90 °. As shown in FIG. 11, the four pressing portions 61a are arranged so as to press the upper surface of the rubber plug 151a of the test tube 151 so as not to seal the central recess of the upper surface of the rubber plug 151a of the test tube 151. ing. As a result, the rubber plug 151a is pulled upward when the piercer cleaning member 61 is raised due to the negative pressure generated when the concave portion on the upper surface of the rubber plug 151a is pressed so as to be sealed. It is possible to suppress the gripping position of the test tube 151 from shifting upward. For this reason, the gripping position of the test tube 151 of the hand member 160 is shifted upward, so that the operation of returning the test tube 151 to the sample rack 150 by the hand member 160 cannot be performed appropriately. Can be prevented. Further, the guide hole 61c has a function of preventing the piercer 11 from being bent when the elongated piercer 11 is inserted into the rubber stopper 151a of the test tube 151.

  Further, the dispensing pipette 12 according to the present embodiment is attached to a mounting plate 32 made of sheet metal so as to be movable in the vertical direction by a resin pipette support 31. Specifically, as shown in FIG. 14, the dispensing pipette 12 is supported so as to be movable in the vertical direction by a pipette support portion 31 including a main body portion 31a having a concave storage portion and a lid portion 31b. . The dispensing pipette 12 is bonded and fixed in a state of being inserted into a resin holding member 38. And between the holding member 38 and the cover part 31b, the compression coil spring 37 for urging the dispensing pipette 12 downward is installed. The compression coil spring 37 is an example of the “biasing means” in the present invention. Moreover, in this embodiment, while the front-end | tip part of the stainless steel dispensing pipette 12 is formed in round shape (R shape), the notch groove | channel 12a is formed in the center part of the front-end | tip part of the dispensing pipette 12. Is formed. Due to the notch groove 12a and the round shape (R shape), the dispensing pipette 12 slides due to the variation in the components of the dispensing pipette 12 and the variation in the thickness of the slide glass 10 when dispensing blood into the slide glass 10. Even when the glass 10 is contacted, an accurate amount can be dispensed. Usually, at the time of blood dispensing, the dispensing pipette 12 is set to dispense at a distance of about 0.5 mm with respect to the slide glass 10.

  As shown in FIG. 5, the second support member 14 is attached with a linear guide composed of a slide rail 33 and a slide body 34 for horizontally moving the dispensing pipette 12 in the front-rear direction. A metal fixing member 39 is attached to the slide body 34. An attachment plate 32 to which the pipette support 31 is attached is attached to the fixing member 39. A driving belt 35 a is fixed to the fixing member 39. As shown in FIG. 7, the drive belt 35a is attached to a pair of pulleys 35b and 35c installed at a predetermined interval. The pulley 35b or 35c is driven by a motor (not shown). Thereby, since the drive belt 35a is driven in the front-rear direction, the dispensing pipette 12 can be moved in the front-rear direction by the linear motion guide including the slide rail 33 and the slide body 34. The attachment plate 32 to which the pipette support 31 is attached is provided with a detection piece 32a for detecting the origin position in the front-rear direction. The second support member 14 is provided with a light transmission type sensor 36 for detecting that the detection piece 32a is positioned at the origin position.

  Moreover, as shown in FIGS. 5-7, the pipette washing member 40 for wash | cleaning the dispensing pipette 12 is provided in the suction dispensing mechanism part 1. As shown in FIG. As shown in FIG. 15, the pipette cleaning member 40 includes a cleaning liquid injection hole 40a, a suction hole 40b for sucking blood adhering to the vicinity of the tip of the dispensing pipette 12, and blood that has been sucked and discharged from the cleaning liquid. And a suction hole 40c for performing the suction.

  As shown in FIGS. 5 to 7, the aspirating / dispensing mechanism unit 1 is provided with a manual sample setting unit 50 for setting a sample (blood) during a manual suction operation without using the transport device 200. ing. The manual sample setting unit 50 is provided with a sample container 51a for storing blood (sample) and a sample container setting unit 51 for storing the sample container 51a. The specimen container installation part 51 is attached to an installation part attachment member 52 made of sheet metal. A linear motion guide composed of a slide rail 53 and a slide body 54 is attached to the side surface of the frame 80.

  An installation portion attachment member 52 is attached to the slide body 54. The installation portion attachment member 52 is attached to the drive belt 55. As shown in FIG. 5, the drive belt 55 is mounted on a pair of pulleys 56 and 57 installed at a predetermined interval. The shaft of the pulley 56 is connected to the shaft of the motor 58. When the drive belt 55 is moved up and down via the pulley 56 by the rotational drive of the motor 58, the sample container installation portion 51 fixed to the installation portion attachment member 52 attached to the drive belt 55 moves up and down. It becomes possible. Further, as shown in FIG. 7, the installation portion mounting member 52 is integrally provided with a detection piece 52 a for detecting the upper end position of the sample container installation portion 51. A light transmission sensor 59 for detecting that the detection piece 52a has reached the upper end position is attached to the side surface of the frame 80 via an attachment member 59a made of sheet metal.

  The detailed structure of the syringe pump 70 will be described with reference to FIGS. 5, 16, and 17. The syringe pump 70 according to the present embodiment is provided with a pump main body 71 made of a resin cylindrical portion. The pump main body 71 is fixed to a support plate 72 made of sheet metal. The upper end of a metal piston 71a is inserted into the piston insertion hole 71b of the pump body 71 so as to be movable up and down. The lower end of the piston 71a is fixed to a vertical movement member 73 made of sheet metal via a connecting member 75. Further, a detection piece 74 for detecting the upper end position of the vertical movement member 73 is attached to the vertical movement member 73. A slide body 76a is attached to the support plate 72, and a slide rail 76b that is movable in the vertical direction with respect to the slide body 76a is attached to the vertical movement member 73. A pair of pulleys 77a and 77b are installed at a predetermined interval, and a drive belt 77c is attached to the pulleys 77a and 77b. A shaft of a motor 78 for rotating the pulley 77b is connected to the shaft of the pulley 77b. Further, a light transmission sensor 79 for detecting that the detection piece 74 has reached the upper end position is attached to the support plate 72.

  Here, in the pump main body 71 according to the present embodiment, as shown in FIG. 17, the piston insertion hole 71b is filled with a reagent such as physiological saline. A seal member 71d made of Teflon for preventing the reagent from leaking from the piston insertion hole 71b of the pump main body 71 is disposed in the opening 71c below the piston insertion hole 71b. In addition, resin guide members 71e and 71f are arranged so as to sandwich the seal member 71d from above and below. The guide members 71e and 71f have a function of suppressing the piston 71a from moving up and down while tilting. Accordingly, it is possible to effectively suppress the disadvantage that the reagent in the piston insertion hole 71b leaks due to wear of the seal member 71d due to the piston 71a moving up and down while being tilted.

  Next, with reference to FIGS. 1 to 8, FIGS. 11 to 15, FIG. 18, and FIG. 19, the operation of the blood smear preparation apparatus 100 according to the present embodiment will be described. First, the cassette 3 loading operation by the cassette loading unit 4 shown in FIG. 2 and the cassette 3 conveying operation to the slide glass insertion unit 6 by the cassette conveying unit 5 are performed. Specifically, first, the cassette 3 is set in the cassette carry-in section 4. Thus, the cassette 3 is transported and fed to the cassette standby position 92 in a state where the transport belt engaging portion 3g (see FIG. 3) of the cassette 3 is engaged with the feed belt 4a of the cassette carry-in portion 4. When the number of cassettes 3 at the cassette standby position 92 of the cassette carry-in unit 4 is equal to or less than a predetermined number (for example, 10), it is detected by the sensor 4b and displayed on the display unit 102 (see FIG. 1). A message such as “Please replenish the cassette” is displayed. The cassettes 3 at the cassette standby position 92 are conveyed one by one to the slide glass insertion unit 6 by the cassette conveyance unit 5. That is, the cassette 3 is transported to the slide glass insertion portion 6 by moving the cassette transport member 5a constituting the cassette transport portion 5 while pushing the side surface portion 3h of the cassette 3 by the transport claws 5b. The cassette conveying member 5a is moved by moving the driving belt 5b with a driving motor (not shown).

  In the slide glass insertion portion 6, first, it is determined by the sensor 6 b whether or not the slide glass 10 is stored in the slide glass storage hole 3 a of the cassette 3. When it is determined that the slide glass 10 is stored in the cassette 3, the cassette 3 is moved to the staining unit 7 as it is. When it is determined that the slide glass 10 is not stored, the following suction dispensing operation is started.

  Here, first, referring to FIGS. 1, 2, 5 to 8, 11 to 15, and 18, the aspirating / dispensing operation of blood smear preparing apparatus 100 when using transport apparatus 200 ( The automatic suction operation) will be described. In the case of the automatic suction operation, the test in which the blood sample is accommodated as shown in step S1 of the flowchart in FIG. 18 in a state where the start switch 102 (see FIG. 1) is pressed and the blood smear preparing apparatus is activated. The sample rack 150 (see FIG. 1) in which the tube 151 is stored is set in the loading unit 201 of the transport apparatus 200. In step S2, the automatic suction start switch displayed on the display operation unit 101 (see FIG. 1) is pressed. As a result, the sample rack 150 is transported to the take-out unit 202 of the transport device 200. Thereafter, in step S3, the hand member 160 of the blood smear preparation apparatus 100 grips the test tube 151 in which the blood in the sample rack 150 is stored. Then, in step S4, the test tube 151 is lifted by raising the hand member 160 and the test tube 151 is stirred by rotating the hand member 160, and then the test tube 151 is placed on the test tube support portion 65 shown in FIG. 151 is arranged.

  In step S5, the piercer 11 is lowered by rotating the motor 26 shown in FIG. 5 in a predetermined direction. Simultaneously with the lowering of the piercer 11, the piercer cleaning member 61 is lowered using the air cylinder 63 with the tip 11 a of the piercer 11 being guided by the guide hole 61 c of the piercer cleaning member 61. Thereby, the rubber stopper 151a of the test tube 151 is pressed from above by the pressing portion 61a of the piercer cleaning member 61. In addition, when the piercer 11 moves up and down, the piercer cleaning member 61 is also moved up and down while the front end portion 11a of the piercer 11 is guided by the guide hole 61c. Thus, the tip end portion of the piercer 11 can be always guided by the guide hole 61c, so that the tip end portion 11a of the piercer 11 has a small clearance (fitting gap with the piercer 11) for each suction step. There is no need to insert into. As a result, when the distal end portion 11a of the piercer 11 is inserted into the guide hole 61c of the piercer cleaning member 61 having a small clearance, the disadvantage that the distal end portion 11a of the piercer 11 cannot be properly inserted into the guide hole 61c occurs. Can be prevented.

  In a state where the rubber plug 151a of the test tube 151 is pressed from above by the pressing portion 61a of the piercer cleaning member 61, in step S6, the piercer 11 is further lowered so that the tip 11a of the piercer 11 becomes the rubber plug 151a of the test tube 151. pierce. Then, after the distal end portion 11a of the piercer 11 breaks through the rubber stopper 151a of the test tube 151 and is disposed in the blood of the test tube 151, blood is circulated from the suction port 11b of the piercer 11 using the syringe pump 70. Aspirate into syringe tube 21. In the suction operation, in the fluid circuit shown in FIG. 8, the valve 15 is opened (on state) and the valve 16 is shut off (off state).

  After completing the blood suction operation, the valve 15 is turned off (off state) and the valve 16 is opened (on state). Further, the motor 26 is rotationally driven in a direction opposite to the predetermined direction to raise the piercer 11. The blood adhering to the outer peripheral surface of the piercer 11 is washed while raising the piercer washing member 61 using the air cylinder 63 in synchronization with the rise of the piercer 11. That is, as shown in FIG. 13, the blood adhering to the outer peripheral surface of the piercer 11 is washed away by injecting the cleaning liquid from the cleaning liquid injection hole 61d and sucking the cleaning liquid from the cleaning liquid discharge hole 61e.

  In step S <b> 7, the blood in the syringe tube 21 is discharged from the dispensing pipette 12 through the pipette tube 20. Specifically, after the dispensing pipette 12 shown in FIG. 5 is moved forward and downward and disposed in the pipette cleaning member 40, blood is discharged from the dispensing pipette 12, and the discharged blood is removed from the pipette cleaning member. Suction is performed from 40 suction holes 40c (see FIG. 15). As described above, by draining blood before dispensing, it is possible to wash away reagents such as physiological saline supplied from the syringe tube 21 to the pipette tube 20 at the time of the previous dispensing, and in the pipette tube 20. Since blood can be acclimated, damage to blood cells can be reduced. Thereby, a high quality smear can be produced.

  Thereafter, in step S8, the valve 15 in the fluid circuit shown in FIG. 8 is opened (on state) and the valve 16 is shut off (off state). The blood remaining in 19 is sucked into the syringe tube 21 again. After the end of the second suction operation, the valve 15 is turned off (off state) and the valve 16 is opened (on state). Next, the blood adhering to the tip of the pipetting pipette 12 before the pipetting is removed by sucking from the suction hole 40b (see FIG. 15) of the pipette cleaning member 40. In step S9, the dispensing pipette 12 is moved forward and downward to the dispensing / smearing position 90 shown in FIG. 2, and then the blood in the syringe tube 21 is dispensed onto the slide glass 10. Drip (dispense) from In this case, a notch groove 12a is provided at the distal end portion of the dispensing pipette 12, and the distal end portion of the dispensing pipette 12 is formed in a round shape (R shape). It is possible to prevent blood from adhering to the tip of the dispensing pipette 12 when dripping into the pipette. After this dispensing operation, the dispensing pipette 12 is moved up, retracted and lowered into the pipette cleaning member 40. Then, the blood adhering to the vicinity of the tip of the pipetting pipette 12 is washed away by injecting the cleaning liquid from the cleaning liquid injection hole 40a of the pipette cleaning member 40 and sucking it from the suction hole 40c.

  A smearing operation by the smearing unit 2 is performed in parallel with the suction dispensing operation by the suction dispensing mechanism unit 1 described above or after the suction dispensing operation. In the smearing unit 2, the slide glass 10 is supplied to the dispensing / smearing position 90, blood dropped on the slide glass 10 is smeared and dried, and printing is performed on the slide glass 10. Specifically, the slide glass 10 stored in the two slide glass storage units 2b of the slide glass supply unit 2a is supplied onto the feed belt 2e by a take-out mechanism and a feed belt 2d (not shown). Then, the slide glass 10 is conveyed to the dispensing / smearing position 90 by the feed belt 2e. The operation of conveying the slide glass 10 to the dispensing / smearing position 90 is performed before the above-described suction dispensing operation. In this state, blood is dropped (dispensed) on the slide glass 10 using the dispensing pipette 12.

  Thereafter, the drag glass 2c is moved so as to contact the slide glass 10 and is reciprocated in the longitudinal direction of the slide glass 10 so that the blood dropped on the slide glass 10 at the dispensing / smearing position 90 is transferred. Smeared. Thereafter, the smeared slide glass 10 is conveyed to the drying position 91a by the feed belt 2e. Then, at the drying position 91a, the blood smeared on the slide glass 10 is dried with cold air by the fan 2f. The slide glass 10 is dried twice at two adjacent drying positions 91a and 91b. Thereafter, the smeared slide glass 10 is moved to the printing unit 2h by the moving piece 2g. In the printing unit 2h, the sample number, date, reception number, name, and the like are printed on the frosted portion 10a of the slide glass 10. Then, the slide glass 10 after printing is moved to the slide glass insertion part 6 side by the moving piece 2i.

  After the above-described suction dispensing operation and smearing operation are completed, the operation of inserting the slide glass 10 into the cassette 3 is performed. Specifically, by rotating the cassette direction moving member 6a in a predetermined direction, the cassette 3 is moved from the vertical position to the horizontal position (the position of the two-dot chain line in FIG. 2) and the slide glass 10 is inserted. Make it possible. In this state, the smeared slide glass 10 is inserted into the slide glass storage part 3a (see FIG. 3) of the cassette 3 by moving the moving piece 2i of the smearing part 2 forward. Thus, the smeared slide glass 10 is stored in the cassette 3. Thereafter, the cassette direction moving member 6a is rotated in the direction opposite to the predetermined direction to return the cassette 3 to the original vertical position. Then, the cassette 3 storing the smeared slide glass 10 is conveyed to the staining unit 7 by the cassette conveying member 5a.

  In the staining unit 7, first, the smeared slide glass 10 is lifted from the slide glass storage hole 3 a of the cassette 3 by the first suction discharge unit 7 c, and at the same time, the May-Grunwald liquid (mainly) is put into the staining solution suction dispensing hole 3 b of the cassette 3. The component is 99% in methanol. Then, after the smeared slide glass 10 is returned to the cassette 3, the cassettes 3 containing the smeared slide glasses 10 are placed one by one on the transport belt 7b by the feeding member 7a. And the cassette 3 is conveyed by the conveyance belt 7b to the 2nd suction discharge part 7d. In the second suction / discharge section 7d, the smeared slide glass 10 is lifted from the slide glass storage hole 3a of the cassette 3, and the blown air from the fan 7j is applied to the smear surface of the slide glass 10 for about 1 second to about 60 seconds. Dry by evaporating the liquid component above. The time (immersion time) from when the smeared slide glass 10 is immersed in the May-Grunwald solution by the first suction / discharge unit 7c to when the slide glass 10 is lifted by the second suction / discharge unit 7d is about 1 second to about 1 second. About 60 seconds.

  Next, a dyeing process (Maygrunwald / Giemsa double dyeing process) is performed. First, in the second suction / discharge section 7d, the slide glass 10 is returned to the slide glass storage hole 3a of the cassette 3 to immerse the smeared slide glass 10 in the May-Grunwald solution again. Thereby, the May Grünwald Giemsa double staining process is started. And while the cassette 3 is conveyed by the conveyance belt 7b, the smeared slide glass 10 is immersed in the May-Grunwald solution as a staining process for about 1 minute to about 5 minutes. Then, after the May-Grunwald liquid is sucked and discharged from the staining liquid suction / dispensing hole 3b of the cassette 3 by the third suction / discharge section 7e, the May-Grunwald diluted liquid is dispensed into the staining liquid suction / dispensing hole 3b of the cassette 3. Noted. And while the cassette 3 is conveyed by the conveyance belt 7b, the smeared slide glass 10 is immersed in the May-Grunwald diluent for about 1 minute to about 5 minutes. Then, the fourth suction / discharge unit 7f sucks and discharges the May-Grunwald diluted solution from the staining solution suction / dispensing hole 3b of the cassette 3, and then dispenses Giemsa solution to the staining solution suction / dispensing hole 3b of the cassette 3. Is done. And while the cassette 3 is conveyed by the conveyance belt 7b, the smeared slide glass 10 is immersed in the Giemsa solution for about 1 minute to about 20 minutes.

  Next, after the Giemsa liquid is sucked and discharged from the staining liquid suction / dispensing hole 3b of the cassette 3 by the fifth suction / discharge section 7g, the cleaning liquid is dispensed to the staining liquid suction / dispensing hole 3b of the cassette 3. Then, the slide glass 10 that has been sucked and dyed is washed with water. Thereafter, the stained slide glass 10 is dried by the fan 7h. Then, the cassette 3 in which the stained slide glass 10 is stored is sequentially sent out from the transport belt 7b to the transport belt 8a side of the storage unit 8 by the feed-out mechanism section 7i. Then, the cassette 3 is fed into the transport belt 8 b of the storage unit 8 by the feeding member 8 a of the storage unit 8. Thereby, the cassette 3 fed from the feeding member 8a is transported to the storage unit 8 by the transport belt 8b and stored.

  Next, referring to FIGS. 1, 2, 5 to 8, 15, and 19, the suction dispensing operation (manual suction operation) of blood smear preparation apparatus 100 when transport apparatus 200 is not used. explain. This manual suction operation is used when the amount of blood serving as a specimen is very small. That is, in the above-described automatic suction operation in which suction is performed by the piercer (suction needle) 11 and dispensing is performed by the dispensing pipette 12, the tube is extended from the piercer 11 to the dispensing pipette 12, so that a certain amount of blood is exceeded. is required. Therefore, it is difficult to use the above-described automatic suction operation when the amount of blood serving as a specimen is very small. Therefore, when the amount of blood serving as a specimen is very small, a manual suction operation in which suction and dispensing are performed by the dispensing pipette 12 is used.

  Specifically, in the case of the manual suction operation, the sample is displayed as shown in step S11 of the flowchart of FIG. 19 in a state where the activation switch 102 (see FIG. 1) is pressed and the blood smear preparation apparatus 100 is activated. The sample blood is set in the sample container 51a for storing the blood (sample) installed in the container setting unit 51 (see FIG. 5). In step S12, the manual suction start switch displayed on the display operation unit 101 (see FIG. 1) is pressed. As a result, in step S13, the motor 58 shown in FIG. 5 is rotationally driven, whereby the sample container setting portion 51 is lowered via the driving belt 55 and the setting portion mounting member 52, and stops at a fixed position. Thereafter, in step S14, the dispensing pipette 12 is advanced and lowered to aspirate blood from the sample container 51a. In step S15, after the dispensing pipette 12 is lifted, retracted and lowered and moved into the pipette washing unit 40, the blood adhering to the tip of the dispensing pipette 12 is sucked into the suction hole 40b (FIG. 15)). Finally, in step S16, after the dispensing pipette 12 is raised, moved forward and lowered and moved to the dispensing / smearing position 90 shown in FIG. 2, blood is dropped onto the slide glass 10 from the dispensing pipette 12 (dispensing). Note) After this dispensing operation, the dispensing pipette 12 is moved up, retracted and lowered into the pipette cleaning member 40. And the blood adhering to the front-end | tip part vicinity of the dispensing pipette 12 is washed away by inject | pouring a washing | cleaning liquid from the washing | cleaning-liquid injection hole 40a (refer FIG. 15) of the pipette washing member 40, and attracting | sucking from the suction hole 40c.

  In the present embodiment, as described above, the first support member 13 and the second support member 14 that are integrally fixed to support the piercer (suction needle) 11 and the dispensing pipette 12, the first support member 13, and By providing a sliding screw 22, a coupling 24, and a driving unit comprising a motor 26 for moving the second support member 14 in the vertical direction, the piercer 11 and the dispensing pipette 12 are moved in the vertical direction by one driving unit. Since the mechanism for driving the piercer (suction needle) 11 and the mechanism for driving the dispensing pipette 12 are provided separately, the structure can be simplified.

  In the present embodiment, as described above, when the piercer cleaning member 61 is provided with the guide hole 61c for guiding the piercer 11, the elongated piercer 11 is inserted into the rubber stopper 151a of the test tube 151 by the guide hole 61c. It is possible to suppress the piercer 11 from being bent. Further, by providing the guide hole 61c in the piercer cleaning member 61, it is not necessary to separately provide a member for guiding the piercer 11, so that the structure can be simplified correspondingly.

  In the present embodiment, as described above, when the piercer cleaning member 61 is provided with the pressing portion 61a that presses the test tube 151 containing the blood, the piercer 11 pierces the rubber plug 151a of the test tube 151. In addition, since the rubber plug 151a of the test tube 151 can be stably supported by the holding portion 61a, the rubber plug 151a of the test tube 151 can be easily broken through by the piercer 11. In addition, by providing the pressing portion 61a integrally with the piercer cleaning member 61, there is no need to separately provide a member for pressing the rubber stopper 151a of the test tube 151 containing the blood, which also simplifies the structure. can do.

  In the present embodiment, as described above, the dispensing pipette 12 is attached to the second support member 14 so as to be movable in the front-rear direction. By moving in the direction, the dispensing pipette 12 can be moved forward and lowered to dispense. Thereby, also when the slide glass 10 is arrange | positioned in the front lower direction of the dispensing pipette 12, the dripping (dispensing) of the blood to the slide glass 10 can be performed easily.

  Further, in the present embodiment, as described above, the dispensing pipette 12 is attached to the second support member 14 so as to be movable in the vertical direction, and the compression coil spring 37 for biasing the dispensing pipette 12 downward. Since the dispensing pipette 12 can escape upward against the urging force of the compression coil spring 37 even when the dispensing pipette 12 descends at the time of dispensing and collides with the slide glass 10, the slide It is possible to prevent the glass 10 from being damaged.

  In the present embodiment, as described above, by providing the pipette cleaning member 40 for cleaning the dispensing pipette 12, the pipette cleaning member 40 cleans the outer periphery of the dispensing pipette 12 for each dispensing operation. Therefore, it is possible to prevent the sample blood before adhering to the outer peripheral portion of the pipetting pipette 12 from being mixed with the subsequent sample blood when dispensing the sample blood later. Thereby, a high-quality smear can be produced.

  The embodiment disclosed this time should be considered as illustrative in all points and not restrictive. The scope of the present invention is shown not by the above description of the embodiments but by the scope of claims for patent, and further includes all modifications within the meaning and scope equivalent to the scope of claims for patent.

  For example, in the above embodiment, the sample suction / dispensing device of the present invention is applied to the suction / dispensing mechanism of the blood smear preparing device. However, the present invention is not limited to this, and the blood smear preparing device. The present invention may be applied to a suction / dispensing mechanism unit of a specimen preparation device other than the above, or may be applied to a sample suction / dispensing device alone.

  Moreover, although the said embodiment demonstrated the case where the support part of this invention was comprised by two members, the 1st support member 13 and the 2nd support member 14, this invention is not restricted to this, and one support member is used. You may make it comprise a support part.

It is the perspective view which showed the whole structure of the blood smear preparation apparatus and conveying apparatus by one Embodiment of this invention. It is the top view which showed the internal structure of the blood smear preparation apparatus by one Embodiment shown in FIG. It is the perspective view which showed the cassette and slide glass which are used for the blood smear preparation apparatus by one Embodiment shown in FIG. It is the perspective view which showed the cassette and slide glass which are used for the blood smear preparation apparatus by one Embodiment shown in FIG. It is the front view which showed the suction dispensing mechanism part of the blood smear preparation apparatus by one Embodiment shown in FIG. It is a side view of the suction dispensing mechanism part by one Embodiment shown in FIG. It is a top view of the suction dispensing mechanism part by one Embodiment shown in FIG. FIG. 6 is a fluid circuit diagram of a suction / dispensing mechanism according to the embodiment shown in FIG. 5. It is the schematic for demonstrating the structure of the piercer of the suction dispensing mechanism part by one Embodiment shown in FIG. It is the figure seen from the front end side of the piercer of the suction dispensing mechanism part by one Embodiment shown in FIG. It is the fragmentary sectional view which showed the state which pressed the rubber stopper of the test tube with the piercer cleaning member of the suction dispensing mechanism part by one Embodiment shown in FIG. It is the perspective view which showed the structure of the piercer cleaning member of the suction dispensing mechanism part by one Embodiment shown in FIG. FIG. 13 is a cross-sectional view taken along the cleaning liquid injection hole and the cleaning liquid discharge hole of the piercer cleaning member shown in FIG. 12. It is sectional drawing which showed the structure of the pipette support part of the suction dispensing mechanism part by one Embodiment shown in FIG. It is sectional drawing which showed the structure of the pipette cleaning member of the suction dispensing mechanism part by one Embodiment shown in FIG. It is the front view which showed the syringe pump of the suction dispensing mechanism part by one Embodiment shown in FIG. It is sectional drawing which showed the internal structure of the pump main-body part of the syringe pump of the suction dispensing mechanism part by one Embodiment shown in FIG. It is a flowchart for demonstrating operation | movement at the time of the automatic suction operation | movement of the suction dispensing mechanism part in the case of using the conveying apparatus by one Embodiment shown in FIG. It is a flowchart for demonstrating the operation | movement at the time of the manual suction operation | movement of the suction dispensing mechanism part in the case of not using the conveying apparatus by one Embodiment shown in FIG.

Explanation of symbols

1 Suction dispensing mechanism (sample suction dispensing device)
2 Smearing section 3 Cassette 4 Cassette loading section 5 Cassette transport section 6 Slide glass insertion section 7 Dyeing section 8 Storage section 11 Piercer (suction section)
12 Dispensing pipette (dispensing part, pipette)
13 1st support member (support part)
14 Second support member (support part)
15 Valve (first valve)
16 Valve (second valve)
22 Sliding screw (drive unit)
24 Coupling (drive unit)
26 Motor (drive unit)
40 Pipette cleaning member 61 Piercer cleaning member 61a Holding portion 61c Guide hole 70 Syringe pump 71 Pump body portion 71a Piston 71b Piston insertion hole 71d Seal member 71e, 71f Guide member 100 Blood smear preparation apparatus 151 Test tube (sample container)
151a Rubber stopper (sealing stopper)
200 Conveyor

Claims (13)

A suction part for sucking the sample;
A dispensing unit for dispensing the aspirated sample;
A support part for supporting the suction part and the dispensing part;
A sample aspirating and dispensing apparatus, comprising: a drive unit for moving the support unit in the vertical direction. The suction part includes a suction needle,
The sample suction / dispensing device according to claim 1, further comprising a suction needle cleaning member that is provided below the suction needle and cleans the suction needle.   The sample suction / dispensing device according to claim 2, wherein the suction needle cleaning member has a guide hole for guiding the suction needle.   The suction needle cleaning member is installed so as to be movable in the vertical direction, and moves up and down as the suction needle moves up and down while guiding the tip of the suction needle through the guide hole. The sample aspirating and dispensing apparatus described in 1.   The sample aspirating and dispensing apparatus according to claim 2, wherein the suction needle cleaning member has a pressing portion that presses a container in which the sample is stored.   The suction part has a triangular pyramid-shaped tip part capable of breaking through a sealing plug of a specimen container in which the sample is accommodated, and a suction needle comprising a suction hole provided in the vicinity of the tip part The sample suction and dispensing apparatus according to claim 1, comprising:   The sample aspirating / dispensing device according to claim 1, wherein the dispensing unit is attached to the support unit so as to be movable in the front-rear direction. The dispensing part is attached to the support part so as to be movable in the vertical direction,
The sample suction / dispensing device according to claim 1, further comprising an urging unit for urging the dispensing unit downward.   The sample aspirating / dispensing apparatus according to claim 1, wherein the dispensing unit includes a pipette having a notch groove at a tip. The dispensing unit includes a pipette,
The sample suction / dispensing device according to claim 1, further comprising a pipette cleaning member for cleaning the pipette. A syringe pump connected to the dispensing unit and the suction unit;
A first valve attached to the support portion and disposed between the suction portion and the syringe pump, and for opening and closing a flow path between the suction portion and the syringe pump;
A second valve attached to the support and disposed between the dispensing unit and the syringe pump, and further configured to open and close a flow path between the dispensing unit and the syringe pump. The sample suction / dispensing device according to any one of claims 1 to 10. A syringe pump connected to the dispensing unit and the suction unit;
The syringe pump is
A pump body having a piston insertion hole;
A piston movably inserted into the piston insertion hole of the pump body,
A seal member disposed in the piston insertion hole of the pump main body, for sealing between the piston insertion hole of the pump main body and the piston;
The sample suction / dispensing device according to claim 1, further comprising: a guide member that is disposed in a piston insertion hole of the pump main body so as to sandwich the seal member and guides the piston.   A specimen preparation apparatus comprising the sample suction / dispensing apparatus according to claim 1.

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