JP2005160428A - Cytoclastic apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cytoclastic apparatus for finely pulverizing various specimens to a state enabling the cytoclasis of the specimen for detecting the DNA of the specimen, generating little oscillation and noise in operation, dispensing with the pretreatment of the specimen, having a compact structure and enabling the cytoclasis at a relatively low cost. <P>SOLUTION: The cytoclastic apparatus has a closed vessel 100 on a holding bed 9. The vessel 100 is oscillated in a state locked with a slide block member 12 by the motion of a crank mechanism 8 on a base plate 7 supported on a bed 6 through a spring 16. The specimen 100a in the closed vessel 100 is stirred together with a hard material 100b and finely pulverized. A balancing force is developed by a hard member 3, etc., in a ring groove 2 formed in the crank mechanism 8 to reduce the oscillation of the whole apparatus. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to an apparatus for breaking and forming a member that needs to detect DNA into a detectable fine powder, and in particular, the member does not require any special pretreatment, and can be easily and reliably pulverized, and the entire apparatus. The present invention relates to a cell disruption device having a simple structure that can be operated with relatively low noise without moving from a set position and the entire structure can be compactly assembled.

Conventionally, a cell destruction apparatus (or method) has been adopted as one means for detecting the DNA of a member. There are various types of device structures. For example, there are devices that perform cell destruction of members using sound waves having a higher frequency than the audio frequency range (about 20 kHz or less). This requires a microphone chip and requires cooling means to reduce the generated heat. Therefore, the device structure is complicated and expensive. On the other hand, as a known technique of the cell disruption device, for example, “Patent Document 1”, “Patent Document 2”, “Patent Document 3” and the like can be mentioned.
JP-A-10-328316 (Claims) JP-A-7-285875 (Claims) JP 2002-301463 (Claims)

Japanese Patent Application Laid-Open No. 10-328316 discloses a “cell destruction method and apparatus” for selectively destroying target cells in a cell aggregate. A semiconductor particle suspension such as titanium oxide particles is injected into the target cells. The cells are destroyed by irradiating with radiation. As described above, this member is limited in the number of members that can be destroyed, and the structure of the device is complicated and expensive. Radiation and leakage prevention means are required, and it cannot be used easily in any place. .
In addition, “Flea allergen production method and flea allergen produced by the method” disclosed in Japanese Patent Application Laid-Open No. 7-285875 of “Patent Document 2” is a method for destroying cells of flea worms in a physiological saline. The body is first crushed with a tissue homogenizer, and then the flea worm body is crushed to the cell level using a cell disruption device that transmits ultrasonic waves. Is required. Therefore, it consists of an expensive and complicated structure, and members are also limited.
In addition, “Paper crushed sewage treatment method and sewage treatment system using the method” disclosed in Japanese Patent Laid-Open No. 2002-301463 of “Patent Document 3” uses a cell disruption device to pulverize trash. The detailed structure is unknown (not disclosed), but it is arranged in the process of the system and is not formed from a single structure.

  The present invention has been invented in view of the above circumstances, is capable of cell destruction of any member, and is composed of a mechanical structure that can be manufactured at a relatively low cost with a compact device structure. An object of the present invention is to provide a cell disruption device that has low noise and movement of the device and that is easy to use.

  In order to achieve the above object, according to the present invention, a member is housed in an airtight container containing a hard material, and a vibration is applied to the member to break down cells to obtain a DNA detectable powder. A cell disruption device, comprising: a base; a base plate supported on the base via an elastic member; a crank mechanism portion placed on the base plate and rotating eccentrically; and the crank mechanism A pedestal that is pivotally supported at one end by the eccentric part of the unit and has the member mounted at the other end, a guide shaft and a screw shaft that are erected on the cradle, and are slidably held on these shafts. It comprises a slide block body pressed against the upper surface of the member and a lock mechanism portion for setting the block body at a predetermined position, and the crank mechanism portion is formed with a ring groove concentrically with the rotation shaft. A plurality of hard members and fluid members in the link groove Characterized in that it is sealed.

  The invention according to claim 2 is characterized in that the plurality of hard members have a total weight corresponding to the weight of the maximum weight mounted on the apparatus.

  According to the cell disruption device of claim 1, the member that needs to detect DNA vibrates due to the eccentric motion of the crank mechanism portion while being housed in the sealed container, and is pulverized by being hit by the hard material in the sealed container. The cell is destroyed. In general, an airtight container containing a member vibrates the entire apparatus greatly due to vibration, and tries to move the entire apparatus, but is provided in an elastic member and a crank mechanism portion installed between a base and a base base. Due to the presence of the hard material or the like in the ring groove, the unbalance force accompanying the eccentric motion is balanced, so that the vibration of the entire apparatus is reduced and the movement of the entire apparatus is prevented. Moreover, it consists of what collected each component in the piled-up state as an apparatus structure, and is summarized compactly as a whole.

  Further, according to the cell disruption device of claim 2, the weight of the hard material sealed in the ring groove is determined in accordance with the heaviest material among the members applied to the device. Of course, it is possible to reduce vibration during vibration of heavy objects, and for lighter objects than this, there is a balance due to the occurrence of natural position movement of the hard member into the ring groove. However, the overall vibration of the device is reduced.

  Hereinafter, embodiments of the cell disruption apparatus of the present invention will be described in detail with reference to the drawings. The general structure of the cell disruption device of the present invention is a structure in which a member, which is a subject, is placed in a sealed container, and the sealed container is vibrated by a crank mechanism portion supported by a base via an elastic member. The member does not require any pretreatment before being stored in the sealed container. Although the entire device vibrates greatly due to the eccentric movement by the crank mechanism, and the movement of the entire device tends to occur in some cases, the vibration absorption by the elastic member and the flow with the hard member in the ring groove provided in the crank mechanism The presence of the member reduces the unbalance force and greatly reduces the overall vibration of the device.

FIG. 1 to FIG. 7 are structural views showing one embodiment of the cell disrupting apparatus of the present invention.
The cell disruption device 1 is roughly divided into a base 6 mounted on the floor 14 via elastic legs 15, a base plate 7 supported on the base 6 via an elastic member, for example, a spring 16, and a base plate 7 A crank mechanism portion 8 mounted on the base, and a cradle that is supported at one end side (lower end side) by an eccentric portion (described in detail later) of the crank mechanism portion 8 and holds the hermetic container 100 on the upper surface. 9, a guide shaft 10 and a screw shaft 11 erected on the cradle 9, a slide block body 12 slidably held on these shafts 10, 11 and pressed against the upper surface of the sealed container 100, and a slide It consists of a lock mechanism 13 and the like for fixing the block body 12 at a predetermined position. Further, the above-described constituent members are accommodated in a cover 17 that encapsulates them. Of course, a power supply unit and a control mechanism unit for operating these components are provided, but their display and explanation are omitted.

  The outline structure of the cell disruption device 1 has been described above. The detailed structure of the cell disruption device 1 will be described below. The elastic legs 15 for supporting the base 6 on the floor surface 14 are composed of four in the present embodiment, and are arranged at the four corners of the square base 6. The portion of the elastic leg that comes into contact with the floor surface 14 is made of a hard rubber body that widens toward the end, so that the base 6 can be elastically supported on the floor surface 14. Further, poles 18 are erected at the four corners of the base 6, one end side of the spring 16 is connected to the upper surface of the pole 18, and the other end side of the spring 16 is connected to the base plate 7. The base plate 7 is made of a flat hard member.

  Next, the structure of the crank mechanism portion 8 will be described with reference to FIGS. The crank mechanism 8 includes a crankshaft body 8b and a motor body 8a that drives the crankshaft body 8b. The motor body 8a includes a motor 19 and a pulley 20.

  As shown in FIG. 3, the crankshaft body 8 b is placed on the base plate 7, a pair of support tables 21, 21 arranged opposite to each other, and an arm on which the rotation shaft 22 is pivotally supported. Portions 23, 23, a pin shaft 24 installed eccentrically between the arm portions 23, 23, a block body 25 pivotally supported at one end by the pin shaft 24, and a cradle fixed to the upper surface of the block body 25 Nine. The cradle 9 is made of a flat dish having an area on which the sealed container 100 can be mounted. A pulley 26 is fixed to the end of the rotating shaft 22, and a belt 27 is installed between the pulley 26 and the pulley 20 of the motor body 8a.

  As shown in FIGS. 3 and 4, the arm portions 23 and 23 are provided with a ring groove 2 concentric with the rotary shaft 22, and a hard member 3 such as a steel ball of a bearing and a fluid member are provided in the ring groove 2. 4 is stored. The hard member 3 is composed of a plurality of small members that float in the fluid member 4 and can move freely in the ring groove 2. Further, the ring groove 2 is sealed by a lid 5 fixed to the arm portion 23 so that the hard member 3 and the flow member 4 do not jump out of the ring groove 2. The block body 25 is thrust-supported by the arm portions 23 and 23 by the thrust plates 28 and 28.

  With the above structure, when the motor 19 is driven, the pulley 26 rotates via the pulley 20 and the belt 27, and the arm portion 23 rotates about the rotation shaft 22. As a result, the pin shaft 24 moves eccentrically, and accordingly, the block body 25 and the cradle 9 move up and down, and the sealing member 100 mounted on the cradle 9 vibrates up and down and is stored therein. Vibration is given to the member 100a and the hard material 100b. As a result, the member 100a is struck by the hard material 100b and collides with each other to be miniaturized, pulverized, and destroyed in a state where DNA can be detected.

  As shown in FIG. 1 and FIG. 2, two guide shafts 10 and a screw shaft 11 extending upward are assembled on the cradle 9 at a position opposite to each other on the cradle. One end side of an arm-shaped support member 29 is connected to the upper ends of the guide shaft 10 and the screw shaft 11 to hold them. Further, the other end side of the support member 29 is pin-supported by a support column 30 standing on the base plate 7.

  Next, the structure of the slide block body 12 will be described with reference to FIGS. The slide block body 12 includes an upper block 12a, a middle block 12b, and a lower block 12c. The upper block 12a and the middle block 12b are connected by a spring 31. The lower block 12c has a flat plate-like shape that comes into contact with the upper surface of the sealed container 100, and is supported on the lower surface side of the middle block 12b via a cushion spring or the like. All these blocks are slidably supported on the guide shaft 10 and the screw shaft 11 as described above.

  Next, the lock mechanism unit 13 will be described mainly with reference to FIGS. The lock mechanism unit 13 includes a moving mechanism body 13b that moves the slide block body 12 to a predetermined position and a lock mechanism body 13a. The lock mechanism 13a includes a cam 32 pivotally supported by the upper block 12a, a lock handle 33 coupled thereto, a cam roller 34 supported by the middle block 12b and engaged with the cam 32, and the like. By rotating the lock handle 33, the cam 32 presses the cam roller 34, thereby pressing the middle block 12b and the lower block 12c downward, and the hermetic container 100 is locked. On the other hand, as shown in FIG. 6, the moving mechanism 13b includes a lever 35 pivotally supported by the upper block 12a on the base end side, a gripping portion 36 fixed to the tip of the lever 35, and the like, and the screw shaft 11 passes therethrough. The hole 37 is formed to penetrate therethrough. FIGS. 7A and 7B show the operation of the moving mechanism 13b. When the lever 35 is rotated downward about its base end, the periphery of the hole 37 engages with the thread 38 of the screw shaft 11. As a result, the lever 35 is locked to the screw shaft 11, and the slide block body 12 is held in that position. On the other hand, as shown in FIG. 7B, when the lever 35 is rotated to a substantially horizontal position, the engagement between the hole 37 and the thread 38 of the screw shaft 11 is released, and the lever 35 becomes free. Thus, the slide block body 12 can smoothly slide up and down along the guide shaft 10 and the screw shaft 11.

Next, the operation of the cell disruption apparatus 1 of the present invention will be described mainly with reference to FIG. The block body 25 and the cradle 9 that are pivotally supported by the pin shaft 24 are moved up and down by the operation of the crank mechanism 8. As a result, the member 100a and the hard material 100b in the sealed container 100 move in a complicated manner, and as a result, the member 100a is pulverized and pulverized. In this case, since a heavy object such as the sealed container 100 is placed on the cradle 9 and the above movement is performed, the device elements on the base 6 vibrate greatly. However, the device element is elastically supported on the base 6 by a spring 16 and the hard member 3 in the ring groove 2 is gathered at a position where the vibration of the device element is reduced as shown in FIG. Applying force. Thereby, the vibration of the device element is reduced. For this reason, since the noise accompanying the vibration of the cell destruction apparatus 1 is reduced and the balance action works, the movement of the entire apparatus hardly occurs. As described above, the member 100a is efficiently pulverized in a quiet state.
FIG. 8B shows a case where the lighter sealed container 100 and the member 100a are used as compared with the case of FIG. In this case, the hard member 3 or the like housed in the ring groove 2 is left as it is, but automatically moves to a position different from the above case to give a balance force corresponding to the state. Act on. Thereby, even if the weight of the airtight container 100 or the member 100a changes, the cell destruction apparatus 1 can perform a quiet motion.

  FIG. 9 shows another embodiment of the present invention. In the case of “Example 1”, the ring groove 2 is formed on the side surface of the arm portion 23, but in the present Example 2, the ring groove 2 is formed by being recessed on the side surface of the pulley 26. Of course, it is the same that the rigid member 3 and the fluid member 4 are accommodated in the ring groove 2 and sealed by the lid 5. As described above, substantially the same effect as in the first embodiment can be obtained.

  The cell disruption device of the present invention is a device for pulverizing a member so that DNA can be detected, but is not limited thereto, and can be used as a device for pulverizing any member. . Thereby, it is used for a wide range of industries. However, there are extremely many types of members, and it is necessary to have a device structure corresponding to this. Therefore, it can be used as a cell destruction device for all members by slightly changing the device structure.

The side view for demonstrating the whole structure of the cell destruction apparatus of this invention. The front view of FIG. FIG. 3 is an axial sectional view mainly showing a detailed structure of a crank mechanism portion in FIGS. 1 and 2. The typical sectional view showing the balance mechanism provided in the arm part of the crank mechanism part of the present invention. The typical sectional view showing the outline structure of the lock mechanism body of the lock mechanism part in the present invention. The typical sectional view showing the outline structure of the lock mechanism part movement mechanism object in the present invention. The expanded partial sectional view for demonstrating the latching effect | action of the moving mechanism body of FIG. The typical sectional view for explaining the operation of the balance mechanism in the crank mechanism part of the cell destruction device of the present invention. The fragmentary sectional view which shows the other Example of the cell destruction apparatus of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Cell disruption device 2 Ring groove 3 Hard member 4 Flow member 5 Lid 6 Base 7 Base board 8 Crank mechanism part 8a Motor body 8b Crankshaft body 9 Receiving base 10 Guide shaft 11 Screw shaft 12 Slide block body 12a Upper block 12b Middle block 12c Lower block 13 Lock mechanism 13a Lock mechanism 13b Movement mechanism 14 Floor 15 Elastic leg 16 Spring 17 Cover 18 Pole 19 Motor 20 Pulley 21 Support base 22 Rotating shaft 23 Arm portion 24 Pin shaft 25 Block body 26 Pulley 27 Belt 28 Thrust plate 29 Support member 30 Support column 31 Spring 32 Cam 33 Lock handle 34 Cam roller 35 Lever 36 Grip part 37 Hole 38 Screw thread 100 Sealed container 100a Member 100b Hard material

Claims (2)

  A cell disruption device that houses a member in a sealed container containing a hard material and applies vibration to the material to destroy the cell to form a DNA-detectable powder. The device is a base and is elastic to the base. A base plate supported via a member, a crank mechanism portion that is placed on the base plate and rotates eccentrically, and one end side is pivotally supported by the eccentric portion of the crank mechanism portion, and the member is mounted on the other end A receiving base, a guide shaft and a screw shaft standing on the receiving base, a slide block body slidably held on these shafts and pressed against the upper surface of the member, and the block body The crank mechanism portion is formed with a ring groove concentric with the rotation shaft, and a plurality of hard members and fluid members are sealed in the link groove. A cell disruption device characterized by being stopped.   2. The cell disruption device according to claim 1, wherein the plurality of hard members have a total weight corresponding to the weight of the maximum weight mounted on the device.

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