JP2005154365A - Deodorant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a deodorant having high safety and capable of suppressing occurrence of an unpleasant body odor including sweat smell, particularly armpit odor. <P>SOLUTION: The deodorant or an apolipoprotein D decomposition-suppressing agent comprises a cis-6-hexadecenoic acid or its salt as an active ingredient. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a deodorant agent that suppresses the generation of unpleasant body odors in humans.

  The body odor extends to the whole body including the smell of sweat, bad breath, scalp odor and foot odor. In particular, the sweat odor is composed of a mixture of the odor typified by “Wakiga” and the acid odor that covers the entire body. In particular, the bad smell is a representative odor that causes disgust, and in recent years, its defense is increasingly desired.

  Apocrine glands that secrete sweat, which is the source of the scent, exist in axillas, areolaes, genital areas, etc., unlike Ecklin sweat glands, but are not localized in those areas, but are widely present in the trunk. In recent years, for example, the trend toward cleanliness has progressed, and it has become a problem to continuously remove such odors.

  In recent years, 3-methyl-2-hexenoic acid (3M2H), which is one of odor molecules, is secreted to the skin surface in a form combined with apolipoprotein D, which is a carrier protein. It has been reported that the carrier protein is decomposed by the presence of bacteria and an unpleasant odor is generated (for example, see Non-Patent Document 2).

  Conventionally, control techniques for unpleasant body odors for humans include sweat control technology using anti-perspirant materials such as zinc paraphenol sulfonate and citric acid, various aluminum and zirconium salts, trichloric acid, benzalkonium chloride, etc. Technology for controlling the growth of unpleasant odor-causing bacteria with materials that have antibacterial activity, converting lower fatty acids, one of the substances causing body odor, into metal salts with zinc oxide (zinc oxide), and deodorizing flavonoids and chlorophyll A technique for deodorizing an unpleasant body odor generated by a material having an effect and a masking technique by a fragrance such as a perfume or a cologne are known.

  However, all of these techniques do not completely eliminate the unpleasant body odor represented by the bad smell, and the effect is not sufficient. Moreover, according to the antibacterial technology, there is a possibility that the primary barrier function of the skin may be lowered by sterilizing the skin resident bacteria in addition to the bacteria causing the unpleasant odor.

On the other hand, cis-6-hexadecenoic acid, which is one of unsaturated fatty acids, is a main component of sebum that constitutes the barrier of the outermost layer of human skin. Recently, a fatty acid derivative derived from sebum containing cis-6-hexadecenoic acid. Has been reported to have antibacterial activity against many gram-positive and gram-negative bacteria (see Patent Document 1). However, it is not known at all that cis-6-hexadecenoic acid has an action of suppressing an unpleasant body odor.
JP-T-2001-502337 Pinkus H; Mehregan AH; Adnexal Nevi and Benign Adnexoid Tumors, in A Guide to Dermatohistopathlogy; 2nd ed, pp528, pp29, by Appleton-Century-Crofts, New York, 1976 Zeng C, et al, Proc Natl Acad Sci USA, 93: 6626-6630, 1996

  An object of the present invention is to provide a deodorant agent that is highly safe and that can suppress the generation of unpleasant body odors, particularly the smell of sweat, including the smell of sweat.

  The present inventors paid attention to the apocrine odor, which is one of the causative substances of unpleasant body odors in humans, and examined the suppression of the production. As a result, cis-6-hexadecenoic acid is a carrier protein of an odor molecule, apolipoprotein D The present invention has been found to be useful as a material that has the effect of suppressing the decomposition of microorganisms by microorganisms and can suppress the generation of unpleasant body odor.

  That is, the present invention provides a deodorant agent containing cis-6-hexadecenoic acid or a salt thereof as an active ingredient.

  The present invention also provides an apolipoprotein D degradation inhibitor containing cis-6-hexadecenoic acid or a salt thereof as an active ingredient.

  Moreover, this invention provides the body odor suppression method characterized by apply | coating cis-6-hexadecenoic acid or its salt to skin.

  Since the deodorant agent and the apolipoprotein D degradation inhibitor of the present invention continuously suppress the generation of unpleasant body odor, they are useful as materials having an excellent deodorizing effect and high safety.

Since cis-6-hexadecenoic acid of the present invention is a main component of sebum constituting the outermost barrier of human skin, it is a completely harmless substance even when administered to animals for a long time.
Cis-6-hexadecenoic acid can be converted into a pharmacologically acceptable salt by a conventional method. Examples of such salts include salts with alkali metals such as sodium salt and potassium salt, calcium and magnesium. Examples include salts with alkaline earth metals such as ammonium salts, amino salts including various amine salts, lysine salts, arginine salts, amino acid salts, guanidine salts, aluminum salts and zinc salts, particularly sodium salts or potassium salts. Is preferred.

  Cis-6-hexadecenoic acid is extracted from a known metathesis reaction or a method using a microorganism (Japanese Patent Laid-Open No. 1-144982, Japanese Patent Publication No. 4-12718), or a vine plant (Tumbergia alata). Although it can be obtained by a method, in particular, according to a method for producing Rhodococcus microorganisms from isopropyl palmitate (Japanese Patent Publication No. 4-12718), it is possible to industrially produce cis-6-hexadecenoic acid. Is possible.

When extracting from slightly fragile, after immersing or heating and refluxing the whole fragile whole plant, stem, flower, leaf or seed with an appropriate extraction solvent, filter, concentrate, freeze dry, etc. as appropriate, concentrate extract or dry It can be obtained as a powder or the like. As extraction solvents, commonly used organic solvents such as methanol, ethanol, propanol, butanol, ether, ethylene glycol, propylene glycol, butylene glycol, petroleum ether, hexane, heptane, cyclohexane, ethyl acetate, acetone, toluene, dichloroethane, chloroform, etc. And water, etc., and one or more of these can be mixed and used.
The extraction treatment can be performed by a conventional method usually at a temperature of about 3 to 100 ° C. for several hours to several weeks, and the extract can be further purified by gel filtration, column chromatography, precision distillation or the like.

  Such cis-6-hexadecenoic acid or a salt thereof, as shown in the Examples below, suppresses the degradation of apolipoprotein D by microorganisms. The substance (odor molecule) that causes apocrine odor, which is the cause odor of human body odor, mainly odor, is considered to be a branched chain unsaturated lower fatty acid such as 3-methyl-2-hexenoic acid (3M2H). It is considered that the odor molecule is conjugated from the apocrine sweat gland by the carrier protein, secreted into the sweat, and the carrier protein is decomposed and released by bacteria living in the skin (see Non-Patent Document 2). And since apolipoprotein D is a carrier protein of the said odor molecule | numerator, if the decomposition | disassembly of such apolipoprotein D can be suppressed, it will be thought that generation | occurrence | production of an unpleasant body odor can be suppressed.

  Therefore, the occurrence of an unpleasant odor can be suppressed by applying the cis-6-hexadecenoic acid or a salt thereof of the present invention to the skin, and a preparation containing an effective amount thereof fundamentally causes the generation of an odor-causing substance. It can be a deodorant agent that can be suppressed.

  The deodorant agent and apolipoprotein D degradation inhibitor of the present invention are used as a preparation for cosmetics, topical medicines or quasi drugs, such as creams, emulsions, lotions, powders, sprays, sticks, sheets or poultices. It is also possible to use several methods in combination.

  The content of cis-6-hexadecenoic acid or a salt thereof when the deodorant agent and apolipoprotein D degradation inhibitor of the present invention are used as cosmetics, topical medicines or quasi drugs is 0.001 to 10% by weight, In particular, the content is preferably 0.01 to 5.0% by weight.

  These cosmetics, external medicines or quasi drugs include various commonly used components such as oils, surfactants, alcohols, chelating agents, pH adjusters, preservatives commonly used as cosmetic ingredients. , UV absorbers, whitening agents, wrinkle improvers, moisturizers, sebum secretion inhibitors, softeners, keratin protective agents, medicinal agents, antioxidants, solvents, etc. Can be formulated in any combination.

  In addition, each of the above preparations contains natural or synthesized porous metal oxide fine particle powder, astringent compounds containing a metal such as aluminum, zirconium, zinc, etc., bactericides, antibacterial agents, antibiotics, etc. By appropriately blending, the deodorant effect can be enhanced.

The deodorant agent of the present invention can control the generation of body odor by applying it to places where an unpleasant odor is likely to occur, such as the foot, heel, head, and genital area. In such a case, the amount of the preparation to be used varies depending on the content of the active ingredient. For example, in the case of a liquid preparation, 1-20 mg per 1 cm ^{2 of the} skin surface, and in the case of a solid preparation, it is preferably 1-50 mg.

Example 1 Apolipoprotein D degradation inhibitory action (1) Preparation of sweat The armpit of a man with apocrine odor was treated with absorbent cotton moistened with 1.5 mL of distilled water, and a liquid (57.5 mL) obtained by squeezing and collecting this was collected. After filtering with a 0.45 μm filter, the concentrate was concentrated with MILLIPOR Centriprep YM-10, distilled water was added again, and the mixture was similarly concentrated with Centriprep YM-10 to remove low molecules. This was concentrated sweat.

(2) 0.04 mL of concentrated sweat prepared by the above method (1), 0.03 mL of 100 mM Tris-HCl buffer, 0.02 mL of distilled water, and 2.0% cis-6-hexadecenoic acid in ethanol 01 mL was added, Brevibacterium epiderumidis washed with pH 7.2, 20 mM Tris-HCl buffer (3 times) was ingested to a final cell mass of about 108 cfu / mL, incubated at 37 ° C. for 24 hours, and then stained with antibody. Went. Ready gel J (separation gel concentration 15%, Bio-Rad) was used for SDS polyacrylamide electrophoresis (SDS-PAGE) of bacterial cell-treated concentrated sweat. For antibody staining, the protein separated by SDS-PAGE is electrically transferred from a gel onto a PVDF filter (Millipore, Immobilon transfer membrane), then the primary antibody is an anti-apolipoprotein D monoclonal mouse antibody (RDI), and the secondary antibody is HRP. Using a labeled anti-mouse Ig antibody (Amersham Pharmacia Biotech), the ECL Plus Western blotting detection system (Amersham Pharmacia Biotech) detects apolipoprotein D, then performs image processing, and the residual ratio of Apolipoprotein D (= sample) Of apolipoprotein D / apolipoprotein D in untreated sweat × 100). The results are shown in FIG.
As cis-6-hexadecenoic acid, one produced according to the method described in Japanese Patent Publication No. 4-12718 was used.

Example 2 Body odor suppression test A deodorant agent (spray) was prepared by blending in the components shown in Table 1 below so as to be 0.2% cis-6-hexadecenoic acid. A spray containing cis-6-hexadecenoic acid was applied to one side of 4 subjects and 0.5 g of control was applied to the other side, and then a side pad was attached to both sides. After 8 hours, the rice pad was collected, and the odor was evaluated by three professional panelists according to the following criteria, and the average odor level was determined. The results are shown in FIG.

5: Smells very strongly 4: Smells strongly 3: Smells 2: Smell can be recognized 1: Smelly smells 0: Does not smell

  By applying 0.2% cis-6-hexadecenoic acid, the side odor level of the subject decreased, and the generation of body odor was suppressed.

By treating the concentrated sweat with Brevibacterium epiderumidis , apolipoprotein D in the concentrated sweat was decomposed and decreased, whereas the addition of cis-6-hexadecenoic acid suppressed the degradation of apolipoprotein D. Thereby, it turned out that generation | occurrence | production of a body odor, especially a bad smell can be suppressed.

FIG. 1 is a diagram showing the apolipoprotein D (ApoD) degradation inhibitory effect of cis-6-hexadecenoic acid. FIG. 2 is a diagram showing the body odor suppressing effect of cis-6-hexadecenoic acid.

Claims (6)

  A deodorant agent comprising cis-6-hexadecenoic acid or a salt thereof as an active ingredient.   The deodorant agent according to claim 1, which suppresses body odor.   The deodorant agent according to claim 1, which suppresses the smell of sweat.   The deodorant agent according to any one of claims 1 to 3, which suppresses an underarm odor.   An apolipoprotein D degradation inhibitor containing cis-6-hexadecenoic acid or a salt thereof as an active ingredient.   A method for suppressing body odor, which comprises applying cis-6-hexadecenoic acid or a salt thereof to the skin.