JP2005144216A - Liquid chemical feed device, soil cleaning system, liquid chemical feed method and soil cleaning method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a liquid chemical feed device capable of suitably feeding the liquid chemical to soil and a liquid chemical need substance such as a wood rotting fungus arranged in the soil, a soil cleaning system, a liquid chemical feed method and a soil cleaning method. <P>SOLUTION: The soil cleaning system S is provided with the liquid chemical feed device 1 provided with a liquid chemical tank 11; liquid chemical feed pipes 40, 51 connected thereto and having a plurality of liquid chemical delivery ports; a complete closing plug for adjusting opening/closing degree of the liquid chemical delivery port; an incomplete closing plug; flow rate adjustment valves 13, 23 for adjusting a feed amount of the liquid chemical; and a nonwoven fabric 91 covering the liquid chemical delivery port and the liquid chemical feed pipe and being permeable with the liquid chemical, and a contaminated soil container-plant composite body 201 provided with a container for cleaning contaminated soil fixed with the wood rotting fungus and the plant planted thereto and arranged in the contaminated soil 101. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a chemical solution supply apparatus, a chemical solution supply method, a soil purification system, and a soil purification method that supply chemical solution to soil or a chemical solution demanding body arranged in the soil.

  Since the Industrial Revolution, we have developed various science and technology, promoted industrialization, and lived a rich and comfortable social life. However, on the other hand, environmental destruction on a global scale has progressed, and has become a serious social problem. One of the causes of environmental destruction is the large number of environmental pollutants produced as a by-product of industrial activities. Removing these environmental pollutants and restoring them to the original environment is a very important issue in the future. ing.

  Generally, chemical methods, physical methods, and biological methods are known as methods for removing environmental pollutants. Among these, the biological method has the most energy-saving and resource-saving processes and generates little waste, and as one of the methods, bioremediation is widely known. In addition, phytoremediation is also known as an environmental restoration technique using plant functions.

Bioremediation is a technology that utilizes the purification function of enzymes produced by microorganisms to decompose and detoxify environmental pollutants, and finally convert them into carbon dioxide, methane, water, inorganic salts, biomass, etc. is there.
When bioremediation is applied to the purification of contaminated soil, (1) it is possible to purify the structure without excavating the soil, and (2) organic substances with a low concentration by utilizing microorganisms with high degrading activity. It has various advantages such as being able to purify compounds and the like in a short time, and (3) low cost and low environmental load, and is attracting attention as a technology for decomposing and removing environmental pollutants.

  Bioremediation methods for remediating contaminated soil include (A) bioreactor methods that immobilize microorganisms that have the ability to resolve environmental pollutants, and (B) various types of microorganisms that originally inhabit contaminated soil and groundwater. Biostimulation method that supplies nutrient substances and enhances their degradation activity, (C) Bioaugmentation method that directly disperses microorganisms with the resolution of environmental pollutants to the contaminated site and introduces them into the contaminated site for purification It is roughly divided into three.

Of these, the currently used method of remediation of contaminated soil by bioremediation mainly provides conditions for enhancing the decomposing activity of microorganisms (degrading bacteria) that inhabit contaminated sites (B) System. Although this method performs purification by adding an appropriate amount of nutrient sources (nitrogen, phosphorus, etc.) to the soil and sending air, it requires a long purification period.
In addition, the purification rate is largely governed by the soil temperature, and a purification effect cannot be expected especially in winter.

Therefore, in order to shorten the construction period, saccharides and the like have been found as substances that increase the activity of microorganisms effective in the soil in a short time (see Patent Document 1).
On the other hand, when (B) the environmental pollutant cannot be decomposed by the biostimulation method, the (C) bioaugmentation method that introduces microorganisms from the outside into the contaminated site may be used. As one of the microorganisms used for such bioremediation and bioaugmentation, wood decay fungi and the like have been studied, and research for practical use is being promoted.

  Wood decay fungi are attracting attention as microorganisms that have a resolution against various environmental pollutants, and depending on the presence or absence of the resolution of lignin, which is a natural hardly decomposable compound, white rot fungi with a resolution of lignin and lignin It is classified as a brown rot fungus with no resolution. The presence or absence of lignin resolution depends on the presence or absence of phenol oxidase production outside the cells. Examples of the phenol oxidase include lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lac).

  That is, white rot bacteria can degrade lignin because they produce phenol oxidase, but brown rot bacteria can't degrade lignin because they do not produce phenol oxidase. However, brown rot fungi are known to mineralize various phenol compounds and are considered to have a resolution for aromatic compounds.

  Many studies on such wood-rotting fungi and environmental pollutants have been made. In 1985, Bumpus et al. Reported that white rotting fungi, Fanerochiate chrysosporium (BKernochaete chrysosporium, BKMF-1767, ATCC42725), DDT (organochlorine insecticide), benzo (a) pyrene (a kind of polycyclic aromatic hydrocarbon), 2, 3, 7, 8-tetrachloro-P-dioxin (a kind of dioxin), 3, 3 ′ The suggestion about the possibility of decomposing a hardly decomposable chemical substance such as 4,4′-tetrachlorobiphenyl (a kind of coplanar polychlorinated biphenyl (Co-PCB)) has been reported (see Non-Patent Document 1).

  With this report as a trigger, research on the degradation of environmental pollutants using the white-rot fungus Fanellochiete chrysosporium has been active and has reached the present.

Moreover, the "contaminated soil purification method" by this inventor is proposed about the purification method using such wood decay fungi (refer patent document 2).
Japanese Patent Laid-Open No. 2002-1303 (paragraph numbers 0009 to 0023, FIGS. 1 and 4) JP 2002-66531 A (paragraph numbers 0013 to 0030, FIG. 1) JABumpus et al., Science, 228: 1434-1436 (1985)

  However, the technique disclosed in Patent Document 2 is a technique for administering plants and wood-rotting fungi such as white-rotting fungi to contaminated soil, and a general method for supplying a large amount of water or nutrients to plants in a short period of time is There was a problem that it was not suitable for wood decay fungi. Therefore, even when wood decay fungi are administered to contaminated soil, the existing methods of supplying water or nutrients inhibit the growth of wood decay fungi, and the production and activity of enzymes that contribute to the degradation of environmental pollutants are both low. There was a problem of being.

  Therefore, the present invention provides a chemical solution supply apparatus, a soil purification system, a chemical solution supply method, and a soil purification method that can suitably supply the chemical solution to soil or a chemical demanding body such as a wood decaying fungus arranged in the soil. The task is to do.

  The invention according to claim 1 as means for solving the above-mentioned problems is a chemical supply device that supplies chemical liquid to at least one of soil and a chemical liquid demanding body arranged in the soil, the chemical liquid supply source, and the chemical liquid A chemical solution supply apparatus comprising: a chemical solution supply pipe connected to a supply source and having a plurality of chemical solution discharge ports.

  Here, in the present invention, the kind of the chemical solution is not particularly limited, and for example, it is supplied to water, nutrients, plants, or microorganisms that promote the growth of plants and microorganisms. Contains substances that promote production.

  According to such a chemical solution supply apparatus, the chemical solution supply pipe is disposed at a predetermined position with respect to at least one of the soil and the chemical solution demand body, supplies the chemical solution from the chemical solution supply source, and discharges the chemical solution from the plurality of chemical solution discharge ports. Thereby, a chemical | medical solution can be gradually supplied to at least one of a soil and a chemical | medical solution consumer.

  The invention according to claim 2 is the chemical liquid supply apparatus according to claim 1, further comprising a chemical liquid discharge opening / closing degree adjusting means for adjusting an opening / closing degree of the chemical liquid discharge opening.

  According to such a chemical solution supply device, the amount of the chemical solution supplied to at least one of the soil and the chemical solution demanding body can be adjusted by the chemical solution discharge opening / closing degree adjusting means.

  According to a third aspect of the present invention, the chemical liquid supply pipe includes a chemical liquid supply main pipe connected to the chemical liquid supply source, and a plurality of chemical liquid supply branch pipes connected to the main chemical liquid supply pipe and having the chemical liquid discharge port. The chemical solution supply apparatus according to claim 1, wherein the chemical solution supply apparatus is provided.

  According to such a chemical solution supply apparatus, the chemical solution is suitably supplied according to the supply range of the chemical solution by appropriately arranging a plurality of chemical solution supply branches corresponding to at least one of the soil and the chemical solution demanding body. be able to.

  The invention according to claim 4 is the chemical solution supply apparatus according to any one of claims 1 to 3, further comprising a flow rate adjusting means for adjusting a supply amount of the chemical solution.

  According to such a chemical solution supply apparatus, the supply amount of the chemical solution can be suitably adjusted by the flow rate adjusting means.

  The invention according to claim 5 further includes a permeable member that covers the chemical solution supply pipe and is capable of penetrating the chemical solution. It is a chemical | medical solution supply apparatus of description.

According to such a chemical solution supply device, the chemical solution discharged from the chemical solution discharge port penetrates the inside of the permeable member, and is supplied to at least one of the soil and the chemical solution demanding body through this osmotic member. That is, the supply range of the chemical solution can be appropriately set according to the size of the permeable member.
Even if the number of the chemical solution discharge ports is small, the supply range of the chemical solution can be set by appropriately changing the size of the permeable member.

  The invention according to claim 6 is the chemical solution supply apparatus according to any one of claims 1 to 5, wherein the chemical solution supply pipe is formed of a stretchable material.

According to such a chemical solution supply apparatus, the chemical solution supply main pipe and the chemical solution supply branch pipe can be appropriately arranged according to the chemical solution supply range for at least one of the soil and the chemical solution demanding body.
In addition, since the chemical solution discharge port formed in the chemical solution supply pipe can be expanded and contracted, for example, when the chemical solution discharge port is plugged to change the chemical solution discharge amount, the size of the plug is appropriately changed and the chemical solution discharge port is changed. The supply amount of the chemical solution can be adjusted by changing the size and shape of the outlet.

  The invention according to claim 7 comprises a plurality of the chemical liquid supply sources and switching means for switching the chemical liquid supplied from the plurality of chemical liquid supply sources. The chemical solution supply apparatus according to claim 1.

  According to such a chemical solution supply apparatus, the chemical solution can be switched and supplied from a plurality of chemical solution supply sources by appropriately switching the switching means.

  The invention according to claim 8 is a chemical solution consumer, a container-plant complex having a container in which wood-rotting fungi have settled and a plant planted in the container, and disposed in soil. The chemical solution supply device according to any one of claims 7 to 7, supplying the chemical solution to the plant to grow the plant, and contacting the root of the plant with the wood-rotting fungus, It is a soil purification system characterized in that the soil in contact with the wood-rotting fungi is elongated in the soil so that the wood-decaying fungi are fixed in the soil and the soil is purified.

  According to such a soil purification system, the chemical solution is supplied to the plant, the plant is grown, the root of the plant contacted with the wood decay fungus, and the root contacted with the wood decay fungus is extended to the soil. It is possible to purify the soil by fixing wood-rotting fungi to the soil.

  The invention according to claim 9 is a chemical supply method for supplying a chemical to at least one of the soil and the chemical demanding body arranged in the soil, wherein a plurality of chemicals are discharged to at least one of the soil and the chemical demanding body. A first step of disposing a chemical solution supply pipe having an outlet; and at least one of the soil and the chemical solution demanding body by discharging the chemical solution from the plurality of chemical solution discharge ports by circulating the chemical solution through the chemical solution supply tube And a second step of supplying the chemical solution to the chemical solution supply method.

  According to such a chemical solution supply method, the chemical solution is discharged to at least one of the soil and the chemical solution demanding body by discharging the chemical solution from a plurality of chemical solution discharge ports of the chemical solution supply pipe disposed in at least one of the soil and the chemical solution demanding body. Can be gradually supplied.

  The invention which concerns on Claim 10 is equipped with the container which the wood decay fungus settled, and the plant planted in the said container, The chemical | medical solution supply which has a some chemical | medical solution discharge port with respect to the container-plant complex arrange | positioned in soil A first step of disposing a tube; a second step of supplying a chemical solution to the container-plant complex by discharging a chemical solution from the plurality of chemical solution discharge ports by circulating the chemical solution through the chemical solution supply pipe; Growing the plant, bringing the root of the plant in contact with the wood-rotting fungus, and extending the root in contact with the wood-rotting fungus to the soil, thereby fixing the wood-rotting fungus to the contaminated soil And a third step of purifying the contaminated soil.

  According to such a soil purification method, the chemical solution is supplied to the plant, the plant is grown, the root of the plant contacted with the wood-rotting fungi is extended to the soil while the plant root is brought into contact with the wood-rotting fungus. It is possible to purify the soil by fixing wood-rotting fungi to the soil.

  ADVANTAGE OF THE INVENTION According to this invention, the chemical | medical solution supply apparatus which can supply a chemical | medical solution suitably to chemical | medical solution consumers, such as the wood decay fungi arrange | positioned in soil and soil, a soil purification system, a chemical | medical solution supply method, and a soil purification method are provided. can do.

Hereinafter, an embodiment of the present invention will be described in detail with reference to FIGS. 1 to 6.
In the drawings to be referred to, FIG. 1 is a perspective view showing an overall configuration of a contaminated soil purification system according to the present embodiment. FIG. 2 is an exploded perspective view showing a chemical liquid supply unit of the chemical liquid supply apparatus shown in FIG. FIG. 3 is an exploded perspective view showing a part of the chemical solution discharge section of the chemical solution supply apparatus shown in FIG. FIG. 4 is an overall perspective view in which a part of the contaminated soil purification container-plant complex shown in FIG. 1 is broken. FIG. 5 is a process diagram showing the manufacturing method of the contaminated soil purification container shown in FIG. 4 in stages. FIG. 6 is a process diagram showing the contaminated soil purification method according to this embodiment in an enlarged manner.

<Contaminated soil purification system>
As shown in FIG. 1, the contaminated soil purification system S according to the present embodiment includes a chemical solution supply device 1 and a contaminated soil purification container-plant complex 201 which is a chemical solution demanding body. In this system, a chemical solution is supplied to a contaminated soil purification container-plant complex 201 (see FIG. 6D) disposed on the soil 101 to purify the contaminated soil 101.

[Chemical solution supply device]
The chemical solution supply apparatus 1 includes two chemical solution tanks 11 and 21 (chemical solution supply sources), and includes a chemical solution supply unit 1A that supplies a chemical solution and a chemical solution discharge unit 1B that discharges the chemical solution.

(Chemical solution supply unit)
As shown in FIGS. 1 and 2, the chemical liquid supply unit 1A includes a first chemical liquid supply system 10, a second chemical liquid supply system 20, a first chemical liquid supply system 10, a second chemical liquid supply system 20, and a chemical liquid discharge unit 1B. And a Y-shaped connector 31 having a three-pronged structure for connecting the two and the chemical solution can be supplied in two systems. However, the chemical solution supply system is not limited to two systems, and may be one system or a plurality of systems of 3, 4, 5,.

  As shown in FIG. 2, the first chemical supply system 10 includes a chemical tank 11, hoses 12 and 14, a flow rate adjustment valve 13 (flow rate adjustment means), and a chemical solution tank 11 installed at a predetermined height position (not shown). It is comprised from a chemical | medical solution tank stand (chemical | medical solution supply source holding means). The hose 12, the flow rate adjustment valve 13, and the hose 14 are connected in this order from the chemical solution tank 11 toward the downstream side, and the downstream side of the hose 14 is connected to the Y-shaped connector 31.

The chemical solution tank 11 has a cock 11a at the lower part thereof. When the cock 11a is opened, the stored chemical liquid flows out by its own weight, and the chemical liquid can be supplied without requiring special power.
Further, the flow rate of the chemical liquid supplied from the chemical liquid tank 11 to the Y-shaped connector 31 is reduced to zero by appropriately rotating the valve body of the flow rate adjusting valve 13 in the direction of the arrow 13A shown in FIG. Can be adjusted as appropriate

  The chemical liquid tank 11, the hose 12, the flow rate adjustment valve 13, and the hose 14 are made of, for example, tetrafluoroethylene in consideration of corrosion resistance, liquid resistance, and durability. The same applies to the devices and members constituting the second chemical solution supply system 20 and the chemical solution discharge unit 1B unless otherwise specified.

  The second chemical solution supply system 20 includes a chemical solution tank 21, hoses 22, 24, a flow rate adjusting valve 23, and a chemical solution tank base (not shown). The flow rate of the chemical solution can be adjusted from the chemical solution tank 21 and supplied to the Y-shaped connector 31 by appropriately rotating the valve body of the flow rate adjusting valve 23 in the direction of the arrow 23A.

Therefore, in the chemical solution supply unit 1A, by appropriately operating the flow rate adjustment valve 13 and the flow rate adjustment valve 23, not only can the amount of the chemical solution supplied to the chemical solution discharge unit 1B be adjusted, but also the type of the chemical solution can be switched. It has become. Furthermore, it is possible to mix with the Y-shaped connector 31 (for example, dilute one of the chemical liquids as water) and supply it to the chemical liquid discharge section 1B.
Here, in this embodiment, the “switching means” in the claims includes the flow rate adjusting valves 13 and 23 and the Y-shaped connector 31.

(Chemical discharge part)
Next, the chemical liquid discharge unit 1B will be described with reference to FIGS.
As shown in FIG. 1, the chemical solution discharge unit 1 </ b> B is a portion that discharges the chemical solution supplied from the chemical solution supply unit 1 </ b> A, and is connected to the chemical solution supply main pipe 40 connected to the downstream side of the Y-shaped connector 31 and the chemical solution supply main pipe 40. Six chemical solution supply branch pipes 51 connected in the form of branches, cross-shaped connectors 61 and 62 and a T-shaped connector 64 for connecting the chemical solution supply main pipe 40 and each chemical solution supply branch pipe 51, and chemical solution discharge ports 43a and 51a (FIG. 3), a completely closed plug 71 and an incompletely sealed plug 72 (medical solution outlet opening / closing degree adjusting means), a most downstream plug 81 for closing the most downstream end of each chemical solution supply branch 51, and a chemical solution supply branch 51 And a non-woven fabric 91 (permeable member) covering the peripheral surface.
In FIG. 1 and FIG. 3, for easy understanding, a portion covered with the nonwoven fabric 91 and a portion not covered are drawn.

  The chemical liquid supply main pipe 40 includes a first chemical liquid supply main pipe 41, a second chemical liquid supply main pipe 42, and a third chemical liquid supply main pipe 43. The first chemical liquid supply main pipe 41 is connected to the downstream side of the Y-shaped connector 31. A cross-shaped connector 61, a second chemical liquid supply main pipe 42, a cross-shaped connector 62, a third chemical liquid supply main pipe 43, and a T-shaped connector 64 are sequentially connected to the downstream side of the first chemical liquid supply main pipe 41.

The second chemical supply main pipe 42 is formed with a chemical discharge outlet having a circular cross section, the third chemical supply main pipe 43 is formed with a chemical discharge outlet 43a, and an incompletely sealed plug 72 (see FIG. 3) is provided. It is attached.
Since the first chemical liquid supply main pipe 41, the second chemical liquid supply main pipe 42, the third chemical liquid supply main pipe 43, and the respective chemical liquid supply branch pipes 51 are made of tetrafluoroethylene as described above, they are flexible (stretchable). The chemical solution supply main pipe 40 and the chemical solution supply branch pipe 51 are arranged in, for example, a king shape, a mesh shape, or the like according to the shape of the contaminated soil 101 in which the chemical solution discharge unit 1B is arranged. It is free.
The lengths of the chemical solution supply main pipe 40 and the chemical solution supply branch pipe 51 can be freely changed according to the range in which the chemical solution is supplied.

  The six chemical supply branch pipes 51 are connected to a cross-shaped connector 61, a cross-shaped connector 62, and a T-shaped connector 64, respectively.

That is, the chemical liquid supply main pipe 40 and the chemical liquid supply branch pipe 51 are detachably connected to each other via a cross-shaped connector 61, a cross-shaped connector 62, and a T-shaped connector 64 (hereinafter collectively referred to as a connector). Yes.
However, the number of the chemical liquid supply main pipe 40, the chemical liquid supply branch pipe 51, and the number of connectors is not limited to this, and can be changed as appropriate. Further, the shape of the connector is not limited to a cross shape or a T shape, and may be changed as appropriate, and may be any shape as long as at least two or more tubes can be connected. Further, the connector may be one that can arbitrarily change the connecting angle of the connected pipes. Furthermore, a connector having an on-off valve may be used as the connector. According to such a connector with an on-off valve, the chemical liquid discharge amount in the chemical liquid discharge unit 1B is adjusted by appropriately adjusting the on-off valve for each connector. Can be partially set, and a desired amount of chemical solution can be supplied to a desired place. In addition, the connector with an injection port which has an on-off valve may be sufficient, and according to such a connector with an injection port, a chemical | medical solution can also be partially injected from the injection port.

  The chemical liquid supply branch pipe 51 is formed with chemical liquid discharge ports 51a having a circular cross section at a predetermined interval so that the chemical liquid can be discharged. Since the chemical solution supply branch pipe 51 is made of tetrafluoroethylene having elasticity as described above, the chemical solution discharge port can be appropriately changed by changing the diameter of the incompletely sealed plug 72 inserted into the chemical solution discharge port 51a. The discharge amount of the chemical solution can be adjusted by freely changing the size of the gap formed between 51a and the imperfect sealing plug 72.

As described above, the chemical solution outlet opening / closing degree adjusting means in the present embodiment is composed of the complete sealing plug 71 having a circular cross section and the incomplete sealing plug 72 having a triangular cross section. By appropriately selecting from 72 and attaching to the chemical liquid discharge port 51a, the chemical liquid discharge amount in the chemical liquid discharge section 1B can be adjusted. More specifically, the chemical solution cannot be discharged from the chemical solution discharge port 51a to which the completely sealed plug 71 is attached. On the other hand, in the chemical solution discharge port 51a to which the incompletely sealed plug 72 is attached, the chemical solution is gradually discharged in small amounts over time from the gap between the incompletely sealed plug 72 and the chemical solution discharge ports 43a and 51a. It is possible.
However, the shape and form of the incompletely sealed plug 72 are not limited thereto, and any shape may be used as long as the chemical liquid can be discharged from the inside of the chemical liquid discharge branch pipe 51 to the outside via the chemical liquid discharge port 51a. For example, an incomplete sealing plug having a through-hole of a predetermined size, an incomplete sealing plug having a stepped outer shape, an incomplete sealing plug having a meshed body, or a non-solid formed from a material capable of absorbing or releasing a chemical solution. It may be a completely sealed stopper.

  Further, the arrangement of the completely sealed plug 71 and the incompletely sealed plug 72 can be appropriately changed in accordance with, for example, the arrangement of the contaminated soil purification container-plant complex 201 that is the supply target of the chemical solution. Further, by appropriately selecting the type and number of plugs to be inserted into the chemical liquid discharge port 51a, the chemical liquid discharge amount can be partially set in the chemical liquid discharge section 1B.

The most downstream plug 81 is detachably attached to the most downstream end of each chemical solution supply branch 51, and in the attached state, the most downstream end of the chemical solution supply branch pipe 51 that seals the most downstream end of the chemical solution supply branch pipe 51 is sealed. Means. And when using the chemical | medical solution supply apparatus 1 so that it may mention later, in the state which made the downstream end of the chemical | medical solution supply branch pipe 51 protrude on the surface of soil, a chemical | medical solution is a chemical | medical solution supply branch pipe by removing the most downstream stopper 81 suitably. It can be easily confirmed whether or not it reaches the downstream end of 51.
However, the most downstream end sealing means for the chemical solution supply branch pipe is not limited to the most downstream plug 81, and for example, a stopper detachably provided at the most downstream end portion of the chemical solution supply branch pipe 51, or the chemical solution supply branch pipe 51. You may use the tube clamp which clamps.

The non-woven fabric 91 (permeable member) is permeable to the chemical solution and covers the peripheral surface of the chemical solution supply branch pipe 51 so as to cover the chemical solution discharge port 51a. Therefore, the chemical solution discharged from the chemical solution discharge port 51a can penetrate the inside of the nonwoven fabric 91 by capillary action. Therefore, as shown by reference numeral 91 </ b> A in FIG. 1, the supply range of the chemical solution can be expanded in the longitudinal direction of the chemical solution supply branch pipe 51.
That is, even if the amount of the chemical liquid discharged from the chemical liquid discharge port 51a is very small, the interval between the adjacent chemical liquid discharge ports 51a is wide, or the number of the chemical liquid discharge ports 51a is small, the liquid is discharged from one chemical liquid discharge port 51a. The medicinal solution thus prepared can be diffused and suitably supplied to the contaminated soil purification container-plant complex 201.
Moreover, it is preferable that the nonwoven fabric 91 is in a wet state. In such a wet state, the chemical solution easily penetrates into the nonwoven fabric 91. Therefore, when the chemical solution supply apparatus 1 is used and coated with the non-woven fabric 91, it is preferable that the non-woven fabric 91 be sprinkled with water, the non-woven fabric 91 is immersed in water, and the like to be wet.
However, the permeable member is not limited to the nonwoven fabric 91, and may be paper or the like.

[Container for contaminated soil-plant complex]
(Constitution of contaminated soil purification container-plant complex)
Next, the contaminated soil purification container-plant complex 201 will be described with reference to FIG.
As shown in FIG. 4, the contaminated soil purification container-plant complex 201 is placed in a pot 241, and the contaminated soil purification container 220 located in the center and the plant planted in the contaminated soil purification container 220. 210, wood-rotting fungi 205 and 205a, and culture soils 242 and 243 filled in the gaps. Reference numeral 205 denotes a wood-rotting fungus fixed on the container body 221 of the contaminated soil purification container 220, and reference numeral 205a denotes a wood-rotting fungus that comes into contact with the root 211 and is dispersed as the plant 210 grows. Reference numeral 242 indicates culture soil laid between the pot 241 and the contaminated soil purification container 220, and reference numeral 243 indicates culture soil laid between the contaminated soil purification container 220 and the plant 210.

  The contaminated soil purification container 220 includes a container main body 221 and a wood-rotting fungus 205 fixed on the lower portion of the container main body 221.

  The container main body 221 makes the plant 210 and the wood decay fungus 205 effectively contact by once planting the plant 210 after fixing the wood decay fungus 205 to the container main body 221. The size and shape of the container body 221 may be appropriately changed according to the size of the plant 210, the area of the contaminated soil to be purified, and the like, for example, a flat or three-dimensional container body can be used. Yes, a known pot may be used.

In the present embodiment, a container body 221 having a shape of a bottomless regular square pyramid is used with its apex vertically downward (see FIG. 5). That is, the container main body 221 has an opening in the vertical direction, and the inner peripheral surface has a rough surface and a mortar shape so that the wood-rotting fungi 205 can be easily fixed.
In addition, a through hole 221a (see FIG. 5) that communicates the inside and the outside of the container body 221 is formed in the lower part of the container body 221, and the root 211 of the plant 210 to be planted passes through the through hole 221a. Thus, it can easily penetrate the outside of the container body 221.

  Further, the container body 221 is made of a peat earth-derived material such as peat moss, a biodegradable polymer such as polylactic acid, polycaprolactone, and bionole, and a biodegradable material such as compost, cellulose, and papers. Therefore, when the container main body 221 is placed on the culture soil 242 and the contaminated soil 101 (see FIG. 6), the container main body 221 is gradually decayed and decomposed. Yes. Further, a gap is formed along with the decomposition and decay of the container body 221 itself. Therefore, the root 211 of the plant 210 can penetrate outside not only through the through hole 221a but also through this gap.

The wood-rotting fungus 205 is fixed in a thin layer on both the inner side and the outer side in the lower part of the container main body 221, and further contacts the root 211 of the plant 210 and is dispersed in the culture soil 242 in a wide range.
The wood decay fungus 205 is not particularly limited in the present invention as long as it is a fungus that produces an enzyme capable of purifying environmental pollutants such as dioxin contained in the contaminated soil 101 (see FIG. 6). It can be used by appropriately selecting from bacteria. Further, as will be described later, the wood-rotting fungus 205 is inoculated and cultured in the medium 223 applied to the container body 221 (see FIG. 5).

Among such wood-rotting fungi 205, examples of white-rotting fungi include Phanerochiate chrysosporium (BKMF-1767, ATCC-24724), Phanerochiete sordida, IFO-312, IFO-312 Ostreata (Preurotus osteoreas, IFO-6515) (Pleurotus), Coriolus versicolor (Coriolus versicolor, IFO-30340) (Paraurotus), Bjerkandera adusta, IFO-4983, etc.
Examples of the brown rot fungi include Tyromyces plustris (IFO-30339) (Grownulatake), Gloeophyllum trabeum (ATCC-11539), and the like.

  Further, the wood-rotting fungus 205 is selected from the group consisting of a wild strain, a mixed strain composed of the wild strain and other microorganisms (mycorrhizal fungi and rhizobia), a cell fusion strain, a mutant strain, and a genetically modified strain. At least one selected. Further, as the wood rot fungus 205, white rot fungi and brown rot fungi may be used alone, or two or more kinds may be used in combination.

The plant 210 is dispersed and brought into contact with the root 211 so as to cover the wood-rotting fungus 205, and then the plant 210 and the root 211 grow while the root 211 is in contact with the wood-rotting fungus 205a. The root 211 extends to 101 (see FIG. 6) to fix the wood-rotting fungus 205, and the type is not particularly limited.
In this embodiment, the ground cover plant that grows so as to cover the ground surface is used in consideration of handling property, density of the root 211 that contacts and disperses the wood-rotting fungus 205, and the like.
Ground cover plants are broadly classified into three types: turfgrass, mainly vegetatively breeding, and mainly seed breeding.

  Examples of turfgrass include, for example, warm turfgrass such as Miyako turf, wild buckwheat, korea shiba, bermudagrass, weeping lovegrass, bahiagrass, creeping bentgrass, Kencookie bluegrass, tall fescue, creeping red fescue, perennial ryegrass, etc. Cold turf grass can be mentioned.

  Mainly vegetatively cultivated are arctoseca, ajuga, kansuge, hosta, shibazakura, shaga, shukkon verbena, silane, budgerigar, sedum, ganbana, haemerocallis, potentilla, pine bug, yablan, rhinoceros herb, and abelia Woodpeckers such as Ivy, Scotch, Iwadaresou, Cotoneaster, Hamagiku, Hypericum, Lubus, Caricinoides, etc. Sasa.

  Examples of seeds that propagate mainly include creeping time, white clover, daikandora, hairy vetch, bird's foot trefoil, and roman chamomile.

  Further, as shown in FIG. 4, the plant 210 grows moderately in the pot 241, and its root 211 extends densely in the culture soil 242. On the surface of the root 211, wood-rotting fungi 205a are attached. Therefore, the wood-rotting fungus 205a is dispersed in the culture soil 242 over a wide range.

(Contaminated soil purification container-plant complex production method)
Next, a method for producing a contaminated soil purification container-plant complex will be described.
The manufacturing method of the contaminated soil purification container-plant complex includes a manufacturing step of the contaminated soil purification container and a manufacturing step of the contaminated soil purification container-plant complex.

First, the manufacturing steps of the contaminated soil purification container will be described with reference to FIG.
As shown in FIG. 5, the manufacturing step of the contaminated soil purification container includes a medium application step (see FIG. 5 (c)) in which the medium 223 is applied to the container body 221, and the medium 223 is inoculated with the wood-rotting fungus 205. It includes a wood-rotting fungus inoculation step (see FIG. 5 (d)) and a wood-rotting fungus culture step (see FIG. 5 (e)) for cultivating the wood-rotting fungus after inoculation.

As shown in FIG.5 (c), the culture medium 223 is apply | coated and provided in the container main body 221 (medium application | coating process). By providing the culture medium 223 in this manner, the wood-rotting fungus 205 can be cultured in a short period of time, and can be reliably and stably inoculated and fixed by the container body 221.
The medium 223 is not particularly limited in the present invention as long as the wood-rotting fungus 205 can be cultured well, for example, potato dextrose or malt extract, glucose, heptone, yeast extract, asparagine, potassium dihydrogen phosphate, Examples thereof include a medium supplemented with magnesium sulfate, thiamine and the like.

Separately in parallel with the medium application step, as shown in FIGS. 5A to 5B, the wood-rotting fungus 205 to be inoculated into the container main body 221 is previously cultured and prepared.
After inoculating the wood-rotting fungus 205 on the plate-shaped agar medium plate 231 (see FIG. 5A), the wood-rotting fungus inoculating plate 232 is prepared by culturing for a predetermined time (FIG. 5B). reference). Thus, the wood-rotting fungus 205 is once inoculated on the agar medium plate 231 that can be preferably cultured and then propagated, and then inoculated on the container main body 221, so that the container main-body 221 is more reliably inoculated with the wood-rotting fungus 205. be able to.

Then, the wood-rotting fungus inoculation plate 232 prepared in this way is divided into appropriate sizes, and a part 232a thereof is inoculated into the medium 223 applied to the container body 221. Then, it culture | cultivates for a predetermined time on a predetermined condition so that the wood decay fungus 205 may settle to the container main body 221. FIG.
The culture temperature is generally in the range of 10 to 37 ° C, preferably 25 to 30 ° C. Moreover, the culture | cultivation days should just fully proliferate | rotate a wood decay fungus, and generally it is the range of 4-20 days, Preferably, it is 5-15 days.
After such culture, a contaminated soil purification container 220 in which the inside and outside of the lower part of the container body 221 are covered with the wood-rotting fungus 205 can be manufactured (see FIG. 5E).

Next, the manufacturing steps of the contaminated soil purification container-plant complex 201 will be described with reference to FIG.
In the manufacturing method of the contaminated soil purification container-plant complex 201, the culture soil 242 is spread in an appropriate container such as a pot 241 to form a recess having an appropriate size, and the contaminated soil purification container 220 is disposed in the recess. A contaminated soil purification container disposing step, a plant planting step of planting the plant 210 in the contaminated soil purification container 220, and a plant curing step of curing the plant 210 while appropriately watering the plant 210 are configured. In each step, the plant 210 can be suitably grown by filling the gaps to be formed with appropriate filling of culture soils 242 and 243.

  The type of the culture soils 242 and 243 is not particularly limited as long as the plant 210 can grow well. Further, the culture soils 242 and 243 may be appropriately mixed with a fertilizer, a soil improvement material, or the like to promote the growth of the plant 210.

  The plant 210 to be planted in the contaminated soil purification container 220 is preferably one that has been pre-cured while appropriately sprinkling water in another pot or the like and has managed the growth status.

  Thus, once the plant 210 is cured under good growth conditions in the culture soils 242 and 243 that do not contain environmental pollutants and are easy to grow, the plant 210 can grow well. With the growth of the plant 210, the plant root 211 grows well and contacts the wood-rotting fungus 205 fixed on the container body 221, and enters the outer culture soil 242 via the through hole 221a of the container body 221. Elongate. Therefore, in the culture soil 242, the wood-rotting fungi 205 come into contact with the root 211 of the plant 210 so as to be suitably dispersed. In this way, the contaminated soil purification container-plant complex 201 is manufactured.

<Chemical solution supply method and contaminated soil purification method>
Then, the contaminated soil purification method including the chemical | medical solution supply method which concerns on this embodiment is demonstrated with reference to FIG.1 and FIG.6.
The contaminated soil purification method includes a chemical solution supply device arranging step of arranging the chemical solution supply device 1 on the contaminated soil 101, a mat-like plant planting step of planting a mat-like mat-like plant 102 so as to cover the contaminated soil 101, and a mat-like plant. An arrangement hole forming step of extracting a core of a predetermined size from above 102 to form an arrangement hole 102a, and a contaminated soil purification container-plant complex in which the contaminated soil purification container-plant complex 201 is arranged in the arrangement hole 102a. Arrangement process, sheet installation process provided so as to cover the sheet S as necessary, supply chemical solution to the disposed contaminated soil purification container-plant complex 201, grow the plant 210, and the wood decay fungi 205, 205a It has a chemical solution supplying step for fixing the contaminated soil 101 and a contaminated soil purification step for purifying the contaminated soil 101.

  First, the environmental pollutant contained in the contaminated soil 101 to be purified is not particularly limited in the present invention. For example, DDT, dicohol, methoxychlor, aldrin, dieldrin, chlordane, 2,4,5-trichlorophenoxy Pesticides such as acetic acid, organochlorine compounds such as trichlorethylene and tetrachloroethylene, polychlorinated phenols such as pencurulophenol, 2,4,5-trichlorophenol, polychlorinated compounds such as 3,4,3 ', 4'-tetrachlorobiphenyl Biphenyls (PCB), dioxins such as 2,7-dichlorodipento-P-dioxin, nitro compounds such as dinitrotoluene, 2,4,6-trinitrotoluene, nitroglycerin, and chloroanilines such as 3,4-dichloroaniline , Naphthalene, a Examples include polycyclic aromatic hydrocarbons such as tracene, benzo (a) pyrene, and phenanthrene, aromatic hydrocarbons such as benzene, toluene, xylene, and ethylbenzene, environmental hormones, azo dyes, and dyes such as triphenylmethane dyes. .

  In addition, the contaminated soil 101 contaminated with such an environmental pollutant is not particularly limited in the present invention. For example, farmland (including paddy fields), incinerator surrounding soil, factory site, crude oil spill accident site, etc. Is mentioned. Moreover, you may apply to the soil which may be contaminated with such an environmental pollutant, for example, the surrounding soil of the newly constructed incinerator.

[Chemical solution supply device placement process]
First, as shown in FIG. 1 and FIG. 6A, the chemical solution discharge unit 1 </ b> B of the chemical solution supply device 1 is arranged on the surface of the contaminated soil 101. In this arrangement, the chemical solution supply main pipe 40 and the chemical solution supply branch pipe 51 of the chemical solution discharge section 1B are arranged in, for example, a mesh shape corresponding to the arrangement of the contaminated soil purification container-plant complex 201. In this arrangement, the downstream end of the chemical solution supply branch pipe 51 protrudes from the surface of the mat-like plant 102 described later.
Furthermore, before disposing the chemical solution discharge unit 1B, the surface of the contaminated soil 101 may be loosened to increase the permeability of the chemical solution.
Furthermore, the depth position in which the chemical solution discharge unit 1B is arranged can be changed as appropriate according to the degree of contamination of the contaminated soil 101, and the chemical solution discharge unit 1B is arranged in a plurality of layers such as a two-layer type in the vertical direction. Also good. In addition, for example, when the surface layer soil near the surface of the earth like dioxin-contaminated soil is targeted, the chemical solution discharge unit 1B and the contaminated soil purification container-plant complex 201 are arranged near the ground surface to supply the chemical solution. Thus, it is possible not only to supply chemical solutions suitable for the wood-rotting fungi 205 and 205a and the plant 210 but also to prevent wasteful leakage of the chemical solution below the surface soil such as a dioxin-contaminated layer.

[Matte plant planting process]
And as shown in FIG.6 (b), the mat-like plant 102 is planted so that the surface of the contaminated soil 101 which has arrange | positioned the chemical | medical solution supply part 1B may be covered. Thus, by covering the surface of the contaminated soil 101, it becomes difficult for the wood-rotting fungi to flow out due to rainwater or the like, and the wood-rotting fungi can be fixed.

[Arrangement hole formation process]
Then, as shown in FIG. 6 (c), in accordance with the position of the chemical solution discharge port 51a of the chemical solution supply unit 1B, the core is extracted using an appropriate means, and the contaminated soil purification container-plant complex 201 is removed. An arrangement hole 102a to be arranged is formed. The size of the arrangement hole 201 corresponds to the size of the contaminated soil purification container-plant complex 201.

[Contaminated soil purification container-plant complex placement process]
After that, as shown in FIG. 6D, the contaminated soil purification container-plant complex 201 is embedded in the arrangement hole 102a and rubbed (see FIG. 6E). At this time, in order to promote the growth of wood-rotting fungi and plants, a culture solution, a medium, or the like may be added as appropriate.

[Sheet installation process]
Then, as shown in FIG. 6 (f), the sheet S is installed so as to cover the surfaces of the contaminated soil purification container-plant complex 201 and the mat-like plant 102 as necessary. By installing the sheet S in this manner, rainwater can be prevented, and wood rotting fungi can be prevented from flowing out due to rainwater. Moreover, since heat retention improves by providing the sheet | seat S, the activity of wood decay fungi becomes high and the contaminated soil 101 can be purified suitably.

[Chemical solution supply process]
Thereafter, the chemical solution is supplied from the chemical solution supply apparatus 1 to the contaminated soil purification container-plant complex 201 to grow the plant 210. In the supply of the chemical solution, since the chemical solution discharge unit 1B is arranged corresponding to the contaminated soil purification container-plant complex 201 as described above, the chemical solution gradually becomes the contaminated soil purification container-plant complex. 201.

When the plant 210 (see FIG. 4) grows, the plant root 211 also grows, and the grown root 211 comes into contact with the wood-rotting fungus 205. Furthermore, when the plant 210 grows, the root 211 extends in the horizontal direction and the vertical direction inside the contaminated soil 101. Then, the wood-rotting fungus 205a adhering to the root 211 is also well settled in a state in which the inside of the contaminated soil 101 is dispersed in the vertical direction and the horizontal direction and is widely dispersed in the contaminated soil 101.
Further, when the chemical solution is being supplied, it is possible to easily determine whether or not the chemical solution has reached the downstream end of the chemical solution supply branch pipe 51 by removing the most downstream plug 81 protruding from the surface of the mat-like plant 102. Can be confirmed.

[Contaminated soil purification process]
And the enzyme which the wood decay fungi 205 and 205a produces acts on environmental pollutants, such as dioxin, and decomposes | disassembles, and the contaminated soil 101 is purified suitably.
As described above, according to the contaminated soil purification method according to the present embodiment, it is possible to suitably fix wood-rotting fungi to the contaminated soil 101, so that the contaminated soil can be effectively purified.

  In addition, according to such a contaminated soil purification method, it is possible to supply chemicals such as water or nutrients suitable for wood decay fungi, by enhancing the activity of wood decay fungi and promoting enzyme production, Degradation of environmental pollutants in the contaminated soil 101 can be enhanced, and the contaminated soil can be suitably purified.

  As mentioned above, although an example was described about suitable embodiment of this invention, this invention is not limited to the said embodiment, In the range which does not deviate from the meaning of this invention, it can change suitably.

In the above-described embodiment, the chemical solution is supplied to the contaminated soil purification container-plant complex 201 to purify the contaminated soil 101. However, the target to which the chemical solution is supplied is not limited thereto, and is simply soil, microorganisms, or the like. The purpose of supplying the chemical solution may be to promote soil improvement, soil environment maintenance, microorganisms and / or plant growth.
Specifically, for example, by using the chemical solution supply apparatus 1, greening including rooftop greening, utilization of mycorrhizal fungi, and the like can be favorably performed. Moreover, in the soil purification by a bio-stimulation method, a chemical solution containing saccharides can be gradually supplied to amplify microorganisms (degrading bacteria) that inhabit contaminated soil. Furthermore, in soil remediation using a bioaugmentation method, water, nutrients, and the like can be efficiently supplied when wood-decaying fungi are introduced into contaminated soil.

  In the above-described embodiment, the chemical discharge outlet opening / closing degree adjusting means is configured by the completely sealed plug 71 and the incompletely sealed plug 72. However, the present invention is not limited to this, and for example, other chemical solutions having different opening areas may be used. It is good also as a chemical | medical solution discharge port open / closed degree adjustment means by forming a discharge port.

  In the above-described embodiment, the switching means is configured to include the flow rate adjusting valves 13 and 23 and the Y-shaped connector 31. However, for example, a three-way valve may be used. When the three-way valve is used in this way, the supply amount of the chemical solution can be adjusted.

  Hereinafter, based on an Example, this invention is demonstrated further more concretely.

(Example)
(1) Contaminated Soil Purification System (1-1) Chemical Solution Supply Device The chemical solution supply device 1 has a chemical solution supply system of the chemical solution supply unit 1A as only one system of the first chemical solution supply system 10, and can adjust the water flow as a chemical solution supply tank 11. 20L cylindrical tank, 30 cm of hose 12, water flow control cock as flow control valve 13, 90 cm first chemical liquid supply main pipe 41 connected to this water flow control cock 41, 30 cm second chemical liquid supply main pipe 42 and 30 cm third chemical liquid Main supply pipe 43, six 45cm chemical supply branch pipes 51 having a 4mm diameter chemical discharge outlet 51a at intervals of 20cm, cruciform connectors 61 and 62, T-shaped connector 64, 4mm diameter complete sealing plug 71 and incomplete sealing A total of 14 plugs 72, eight 30 cm × 30 cm non-woven fabrics 91 and six most downstream plugs 81 were used, and the chemical solution discharge part 1B was shaped like a king. The hose 12, the first chemical liquid supply main pipe 41, the second chemical liquid supply main pipe 42, the third chemical liquid supply main pipe 43, and the chemical liquid supply branch pipe 51 are made of tetrafluoroethylene.

(1-2) Chemical Solution The chemical solution was appropriately selected from two types of water and 100 μM manganese sulfate aqueous solution (MnSO ₄ ).

(2) Container for contaminated soil purification-plant complex (2-1) Manufacture of container for purification of contaminated soil (2-1-1) Preparation of wood-rotting fungi for inoculation White-rotting fungi [Fanerochiate Chrysosporium BKMF-1767, ATCC-42725] was used. The white rot fungus was inoculated in advance on an agar medium made of 2% potato dextrose (PDA) and cultured at 28 ° C. for 7 days to prepare a white rot fungus inoculum plate for inoculation in the form of a mycelium.

(2-1-2) Application of container body and medium As a container body, the entrance has a square with a side length of 8 cm, the bottom has a square with a side height of 6.5 cm, and the height is 6 cm. It was used. Moreover, the container main body used what was formed from the biodegradable material which has a sphagnum deposit and has beet moss which was excellent in water permeability and water retention.
Then, 1% malt extract (manufactured by Difco) as a culture medium was applied to the container body to a height of 4 cm from the bottom of the container body and moistened.

(2-1-3) Inoculation / culture of white rot fungus And the white rot fungus inoculation plate was divided into 3 mm × 3 mm size and inoculated at the bottom of the container body. Then, the culture | cultivation was performed at 28 degreeC for 7 days, and the container for contaminated soil purification from which the outer side and the inner side were covered with the white rot fungus from the lower part of the container main body to about 4 cm was manufactured.

(2-2) Manufacture of contaminated soil purification container-plant complex (2-2-1) Miyako turf (plant)
As the plant, Miyako turf, a kind of warm land type turf grass, was used. Miyako turf was pre-planted in soil, cured for 3 weeks with appropriate watering, and adjusted for growth conditions.

(2-2-2) Planting of Miyako-turf And then, red cherries were spread over a case of an appropriate size to form a depression in the red cherries, and a contaminated soil purification container was placed thereon. Thereafter, separately prepared Miyako turf was extracted from the growing soil with a core having a diameter of 6 cm and a depth of 4 cm, planted in a contaminated soil purification container, and rubbed. At the time of this rubbing, the formed gap was appropriately filled with red crust. Then, it was cured while gradually supplying water to produce a contaminated soil purification container-Miyako turf (plant) complex.

(3) Contaminated soil As the contaminated soil, incinerated ash and general soil were mixed to prepare dioxin-contaminated simulated soil containing dioxin, which was spread in a 90 cm square test zone with a thickness of 10 cm. The dioxin concentration contained in the dioxin-contaminated simulated soil is 800 (pg-TEQ / g). “TEQ” means an equivalent amount of toxicity, and the amount of dioxins converted to the amount of 2, 3, 7, 8 tetratetrachlorodipentazoparadioxin having the strongest toxicity among dioxins. It is a symbol which shows expressing as.

(4) Arrangement of Chemical Solution Supply Device and Contaminated Soil Purification Container-Miyako Turf Complex A chemical supply device was arranged after fertilizer was seeded and sprinkled on dioxin-contaminated simulated soil. Then, by covering the chemical solution discharge part of this chemical solution supply device and dioxin contamination simulated soil, plant a mat-shaped coconut turf (hereinafter referred to as a mat-shaped miyako turf), and curing for 2 weeks while watering appropriately, Dioxin contaminated simulated soil was covered with Miyako turf.
Thereafter, an appropriately sized core was extracted from the mat-like Miyako turf to form a placement hole, and a contaminated soil purification container-Miyako turf complex was planted. The gap formed at the time of planting was appropriately filled with red crust.

(5) Supply of chemical solution Water was supplied at 10 L / day as a chemical solution from the chemical solution supply apparatus in such a state. In addition, water was appropriately supplied instead of the 100 μM manganese sulfate solution. By this supply, manganese ions in the manganese sulfate solution activate an enzyme (manganese peroxidase) reaction by white rot fungi.
In this way, the Miyako turf was cured and grown while supplying the chemical solution, and white rot fungi were fixed in the dioxin-contaminated simulated soil.

(6) Evaluation (6-1) Observation of the growth behavior of Miyako turf and white rot fungi The Miyako turf complex container was digged up 2 weeks and 4 weeks after planting in the simulated soil of dioxin contamination. The growth of white rot fungi was visually observed and evaluated. In parallel with this, the growth status was also evaluated by microscopic observation.

(6-2) Evaluation of enzyme activity produced by white rot fungus The enzyme activity produced by white rot fungus was evaluated by a dye evaluation method. The dye evaluation method is a method for evaluating the activity of an enzyme with a dye, and a dye solution and a dye table were used.

(6-2-1) Dye solution.
The dye solution contains 10 g of glucose, 0.5 g of MgSO ₄ , 22 g of KH ₂ PO ₄ , 100 mg of CaCl ₂ , 10 mL of the trace element solution, and 20 mM sodium acetate (pH 4.5) as a buffer per liter. A phenol red solution (a buffer containing 1 mL of a 2 g / 100 mL solution was added to the solution was used as the balance. Table 1 shows the composition per 1 L of the dye solution.
Note that the trace element solution, per 1L, the MgSO ₄ 3 g, MnSO ₄ and 0.5g, the _{NaCl 1.0g, FeSO 4 · 7H 2} O and 0.1 g, the CoCl ₂ 0.1 g, ZnSO 0.1g of ₄ · 7H ₂ O, 0.1g of CuSO ₄ , 10mg of AlK (SO ₄ ) ₂ · 12H ₂ O, 10mg of H ₃ BO ₃ , 10mg of Na ₂ MoO ₄ · 2H ₂ O and It is a solution prepared by adjusting 1.5 g of acetate as distilled water. Table 2 shows the composition per liter of the microelement solution.

(7-2-2) Dye Table 1 to No. 14 colors are arranged from yellow to crimson corresponding to 14, and color mode RGB is used. 1 to No. For No. 10, the compounding ratio was fixed at 100 for red, 20 for blue, and 10 for green from 0 to 100. 1 to 11 colors were produced.
No. 12 to No. For No. 14, the green was fixed to 0, the blue was fixed to 20, and the red was changed to 90, 80, 70, and No. 14 was changed. 12-14 colors were made. No. 1-No. Table 3 shows the compounding ratio of No. 14. In Table 3, the higher the activity of the enzyme produced by white rot fungi, the higher the No. Exhibit 14 dyes.

(6-2-3) Evaluation Procedure A predetermined amount of soil was collected from the soil after two weeks had elapsed after placing the examples and inoculating the white rot fungi. And after adding this in the 15 mL centrifuge tube which poured 3 mL of pigment | dye solutions, it mixed by vibrating for 30 seconds. After mixing, the color change of the dye solution was observed using the dye table shown in Table 3, and the enzyme produced by the white rot fungus was evaluated based on the color change.

(Comparative example)
As a comparative example, a contaminated soil purification container-miyako turf complex was planted in dioxin-contaminated simulated soil by the above-described method, and was roughly controlled. In other words, it was not managed at all except that the supply of water or the like was performed by spraying 10 L at a time.

(7) Evaluation results (7-1) Growth behavior evaluation results In the example using the chemical supply device, the roots of Miyako turf grow well in the dioxin-contaminated simulated soil, and are widely applied to the soil in a state of being entangled with the roots. The white rot fungus settled in can be observed. On the other hand, in the comparative example in which the chemical solution was sprayed, it was observed that the white rot fungi were not sufficiently established.
Therefore, it was confirmed that the white rot fungus can be suitably established in the contaminated soil by using the chemical solution supply apparatus.

(7-2) Evaluation Results by Dye Evaluation Method From the soil sampled from the dioxin-contaminated simulated soil in which the examples were arranged, No. 1 in the dye table shown in Table 3 was obtained. Eight colors were shown. On the other hand, the soil to which the comparative example was administered was No. in the pigment table. 5 was shown.
Therefore, it was found that the activity of the enzyme produced from the white rot fungi contained in the dioxin-contaminated simulated soil administered with the example was higher than that of the comparative example. Therefore, it has been confirmed that by using the chemical solution supply apparatus, it is possible to appropriately produce an enzyme and to prepare an environment for promoting the activation of the enzyme.

It is a perspective view which shows the whole structure of the contaminated soil purification system which concerns on this embodiment. It is a perspective view which decomposes | disassembles and shows the chemical | medical solution supply part of the chemical | medical solution supply apparatus shown in FIG. It is a perspective view which decomposes | disassembles and shows a part of chemical | medical solution discharge part of the chemical | medical solution supply apparatus shown in FIG. It is the whole perspective view which fractured | ruptured a part of container for contaminated soil-plant complex shown in FIG. It is process drawing which shows the manufacturing method of the container for contaminated soil purification shown in FIG. 4 in steps. It is process drawing which expands and shows the contaminated soil purification method which concerns on this embodiment in steps.

Explanation of symbols

S Contaminated soil purification system 1 Chemical liquid supply device 1A Chemical liquid supply part 1B Chemical liquid discharge part 11, 21 Chemical liquid tank (chemical liquid supply source)
13, 23 Flow rate adjusting valve (flow rate adjusting means, switching means)
31 Y-shaped connector (switching means)
40 Chemical liquid supply main pipes 43a, 51a Chemical liquid discharge port 51 Chemical liquid supply branch pipes 61, 62 Cross-shaped connector 64 T-shaped connector 71 Completely sealed plug (chemical liquid discharge port opening / closing degree adjusting means)
72 Incompletely sealed stopper (medical solution outlet opening / closing degree adjusting means)
91 Nonwoven fabric (permeable member)
101 Contaminated soil 102 Plant 201 Container for contaminated soil purification-plant complex (chemical solution consumer)
205, 205a Wood decay fungus 210 Plant 211 Root

Claims (10)

A chemical supply device for supplying a chemical to at least one of the soil and the chemical demanding body arranged in the soil,
A chemical supply source;
A chemical supply pipe connected to the chemical supply source and having a plurality of chemical discharge ports;
A chemical solution supply apparatus comprising:   The chemical solution supply apparatus according to claim 1, further comprising a chemical solution discharge opening / closing degree adjusting means for adjusting an opening / closing degree of the chemical solution discharge port. The chemical supply pipe is
A chemical supply main pipe connected to the chemical supply source;
A plurality of chemical liquid supply branch pipes connected to the main chemical liquid supply pipe and having the chemical liquid discharge port;
The chemical solution supply device according to claim 1 or 2, further comprising:   The chemical solution supply apparatus according to any one of claims 1 to 3, further comprising a flow rate adjusting unit that adjusts a supply amount of the chemical solution.   The chemical solution supply apparatus according to any one of claims 1 to 4, further comprising a permeable member that covers the chemical solution supply pipe and is capable of penetrating the chemical solution.   The said chemical | medical solution supply pipe | tube was formed from the material which can be expanded-contracted, The chemical | medical solution supply apparatus of any one of Claims 1-5 characterized by the above-mentioned. A plurality of the chemical supply sources;
Switching means for switching the chemicals supplied from the plurality of chemical supply sources;
The chemical solution supply apparatus according to any one of claims 1 to 6, further comprising: A chemical liquid consumer, which has a container in which wood-rotting fungi have settled and a plant planted in the container, and is a container-plant complex placed in soil,
A chemical solution supply device according to any one of claims 1 to 7,
Supplying the medicinal solution to the plant, growing the plant, bringing the root of the plant into contact with the wood-rotting fungus, and extending the root in contact with the wood-rotting fungus to the soil; A soil purification system characterized in that bacteria are fixed on the soil and the soil is purified. A chemical supply method for supplying a chemical to at least one of the soil and the chemical demanding body arranged in the soil,
A first step of disposing a chemical liquid supply pipe having a plurality of chemical liquid discharge ports on at least one of the soil and the chemical liquid demand body;
A second step of supplying the chemical liquid to at least one of the soil and the chemical liquid demanding body by discharging the chemical liquid from the plurality of chemical liquid discharge ports by causing the chemical liquid to flow through the chemical liquid supply pipe. The chemical solution supply method. A first step of disposing a chemical solution supply pipe having a plurality of chemical solution discharge ports with respect to a container-plant complex provided with a container in which wood-rotting fungi are fixed and a plant planted in the container; ,
A second step of supplying the chemical solution to the container-plant complex by discharging the chemical solution from the plurality of chemical solution discharge ports by circulating the chemical solution through the chemical solution supply pipe;
Growing the plant, bringing the root of the plant in contact with the wood-rotting fungus, and extending the root in contact with the wood-rotting fungus to the soil, thereby fixing the wood-rotting fungus to the contaminated soil A third step of purifying the contaminated soil;
A soil purification method characterized by comprising:

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