JP2005137857A - Bone forming material made from ash collected after rice bran incineration and method for manufacturing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To develop a raw material of apatite as a raw material of an artificial bone material containing bone-forming cells. <P>SOLUTION: A calcium phosphate raw material with much calcium phosphate, high purity, and uniformity is obtained by ashing rice bran and by removing an alkali soluble component and a carbon component. By mixing the calcium phosphate with calcium chloride, magnesium chloride, etc., kneading with water to make clay, pouring the clay into a mold, drying molded clay, and calcining in an incinerator at 800 to 1,200°C, hard artificial apatite bones are formed. A porous apatite bone forming body is made by carbonizing and ashing a mixed burnable material. A raw material of the rice bran ash is also used as a desiccant for wakame seaweed, kelp, etc., a basement for cultured seaweed for enrooting in the sea, and a nutritional supplement for fermenting bread. It is also used with plaster and as a building material of a bulking material of cement. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

Rice bran retained 7-8% phosphoric acid and was used as a phosphate fertilizer. However, the amount of pesticides increased, white rice became more refined and the amount of rice bran further increased, and the effective use of excess rice bran was required.
On the other hand, the elucidation of the cells of the human tissue has gradually become clear, the behavior of genes in the stem cells has also become clear, and the osteogenesis by stem cells has been developed for the treatment of osteoporosis in the elderly, combining apatite with calcium phosphate It became clear that it had a rapid bone-forming effect due to, and quantitative production of apatite raw materials became a problem, and rice bran ashing became a focus of attention. This incinerated ash of rice bran has a high content of phosphoric acid and calcium, and the rice fir shell has a low amount of phosphoric acid and a high content of oxalic acid, so it is not very interesting as a raw material for apatite. However, rice bran contains 7-8% phosphoric acid as an apatite raw material and can be made suitable by adjusting calcium. Incineration ash is particularly suitable for modeling because it is obtained in the form of nanoparticles. The chemical formula of apatite is Cao-Ca ₂ Po ₅ .

  In recent years, elucidation of molecular biochemistry and gene and protein structure has made it possible for humans to elucidate the mechanism of osteogenesis, and the development of ceramic processing has made it possible to freely mold and fire artificial apatite. The artificial apatite ceramic porous artificial bone In addition, the development of storage technology by rapid freezing with liquid gas has made it possible to improve the processing of human bone peculiar to the elderly. Development of raw materials for artificial apatite has also become necessary.

We decided to process and use incinerated ash obtained by baking rice bran as a raw material for apatite raw material with constant quality.
Many human bones have legal restrictions even when they are excessive, and there are variations in the bones of beasts and fishes, which have problems in processing and use. In addition, natural phosphoric acid soot contains a lot of unnecessary impurities and has a drawback that it takes time and effort for purification.

  The present invention uses 15% of rice bran as the reason for using rice bran raw material in Japan, and a huge amount is obtained when Southeast Asia, China, Korea, the United States and Canada are added. It is easy to process by making it from incinerated ash because it is stable as a raw material for apatite.

This apatite molding process, particularly the porous process in which stem cells absorb and proliferate by making them porous, is a problem. In addition, the osteoclast and apatite porous molded body, which have problems such as heating temperature and firing time, and the simple shape and size of human bone, also require compressive strength of 150 kg / cm ³ or more. It is necessary to further sinter the porous material. For this purpose, oxalic acid, alumina, calcium, magnesium and iron must be mixed and melted. However, this apatite, which is easy to sinter by lowering the sintering temperature, may be used by pulverizing animal aggregate apatite, but it is usually calcined and pulverized to produce magnesium chloride or calcium chloride. What was molded by roughly kneading with 10% aqueous solution, 1% popal resin solution and fiber powder, dried and baked and cured at 800-1000 ° C. If 5% is added, the firing temperature can be increased to 1000 ° C.
In order to make this porous, it is necessary to pay attention to the fact that a high impregnation rate is important for culturing osteoblasts and impregnating them by injection.

A rice cake is heated and incinerated to obtain apatite. However, in order for the bone-forming cells to impregnate porous apatite and perform bone-forming action, it is necessary to make the content of the apatite uniform. In addition, it is necessary that the apatite molded product has a compressive strength of 150 kg / cm ³ or more. For this reason, the firing temperature needs to be sufficiently high, but if the temperature rises and becomes vitrified, part of the porous material may be crushed. is there. For that purpose, calcium, magnesium, alumina, iron, and oxalic acid need to be appropriately blended to increase the tissue binding.

The challenge to solve the problem

  When rice bran is used as an apatite raw material, about 12% fatty acid is recovered and reused in order to reduce the production cost. In order to remove pesticides, rice bran is dispersed in an aqueous solution, and 2 to 3% of pesticides are volatilized in a microwave oven, followed by Bacillus fermentation. It isolates and separates protein cells and fat cells to prevent the generation of peroxidized fat, heats and dries them in a microwave oven so that they can be evenly burned, introduces them into a rotary kiln and burns them, and simultaneously collects apatite ash. Since this is alkaline, it is soaked in water, neutralized with calcium chloride or magnesium chloride, washed with water, separated and dried to make a powder, and pulverized to make an apatite raw material.

For molding, fiber powder and popal water solution added to apatite and masticated are compressed into a mold with a molding machine, taken out and dried naturally and introduced into a baking furnace at 800 ° C ~ It is fired at 1200 ° C. to form a molded product, and further fired and fired to produce a porous artificial aggregate, which is washed with water to make a bone-forming porous ceramic to be sterilized. In order to increase the strength, it is necessary to vitrify the ceramic, and if the incineration temperature is increased, it will become vitrified.However, since the porous structure is broken, pickling may be necessary. In order to obtain a compressive strength of 150 kg / cm ³ , it may be vitrified and pickled to form porous glass.

The invention's effect

A rice bran is used as a raw material and baked to make it ashed, and the apatite is made of nano-fine particles, and the dealkalized product is obtained with a high purity apatite.
In order to add a porous agent to form this with water and a bonding agent, the addition of fibrous powder makes the impregnation more intense, and this includes calcium chloride, magnesium chloride, zirconium, sodium oxalate, collagen, etc. When used, a material having a compressive strength of 200 kg / cm ³ or more is obtained, and the processing becomes easy. In addition, since the sintering temperature is raised in a porous state, the strength is further increased, and it works sufficiently as a resident of osteoblast. In addition, rice bran apatite is composed of components close to human bones, so there is little distortion during bone formation. There are few obstacles even when dissolved.

With the use of rice bran incineration ash that can be used for many patients with osteoporosis due to longevity, a large amount of artificial aggregate can be supplied.
Apatite made from incinerated ash of rice bran can be of quantitatively equivalent quality. Moreover, when using it for a human body, it can be ingested as a health food as a nanopowder as well as processed ceramics of artificial bone coarse material. In addition, since it can be mixed and injected into a stem cell group, it is used not only for joint bones but also for vertebral bones, and also for coarse materials for artificial dentures. It is also used as a bread and baking powder material and has a wide range of uses.
If this osteoblast is cultured by a conventional method, the fluidity is maintained, and the surplus cells proliferate are stored in a liquid gas in a frozen state, so that the change is reduced.

The incinerated ash obtained by burning and ashing the rice bran as a raw material for apatite contains alkaline soda and potash, so it must be washed with water to make it weakly alkaline or neutral, and the ash must be separated and dried. When incinerated, 7-8% phosphoric acid is contained in rice bran, and calcium, magnesium, and iron naturally impregnated in the rice bran are reacted by firing to form calcium phosphate in part. When soda and potash are present, a hydroxyl group is present in calcium together with a phosphate group. In order to make it porous, it is necessary to mix nano-ground powder of rice bran with this incinerated ash and mix alumina, oxalic acid, magnesium and calcium as reinforcing agents for forming a porous layer to increase the melting point. Moreover, the porosity needs to be formed uniformly.
For this purpose, the rice bran mixed to make it porous is kneaded so that it is evenly dispersed, but in order to increase the strength, the mold is compressed and magnesium chloride, calcium chloride, popal and collagen are used as binders. When added and mixed, a dense porous material is formed and formed by firing. At that time, the rice bran powder is carbonized at 800-1200 ° C. at high temperature and is ashed after carbonization to form a porous reinforced film. This porosity is strengthened by apatite binding.
The specific gravity of 2.2 is reduced to 1.7 by making it porous. Moreover, this porous bubble forms an open cell.

FIG. 1 shows a process diagram of a method for producing an apatite powder fired from rice bran and an artificial porous apatite formed and fired. The rice bran flour (A) is charged from the inlet (3) of the autoclave (2) by the conveyor (1) and sealed, and the hexane alcohol solvent is supplied from the pipe (6) to the autoclave by the pump (5) from the hexane alcohol tank (4). The deoiled fat which introduce | transduced and circulated the solvent from the pipe (6 ') is performed. If necessary, it heats with a heater (7) and extracts fats and oils. (9) enters the fractionator (8) through the temperature controller, enters the fractionated oil and fat tank (10), and the solvent is introduced from the pump (11) to the autoclave (2) through the pipe (12).
The degreased rice bran is separated from the pipe (13) by the filter press (14) and the solvent is stored in the tank (16) to recover the solvent. The rice bran (A ′) is dried by the moving vehicle (17) (18). ), The rice bran (A ′) that has been dried through is introduced into an autoclave (21), a nutrient is added to water, fermented bacteria (Bacillus) are added, and fermented at 260 ° C. for 30 minutes. The fermented rice bran (A ″) was separated by a filter press (24), the washing water was pushed in with the pump (27), and the washed rice bran (A ″) was sent to the dryer (18). 29) is put into the hopper (30) and incinerated by the burner (30 '). The incinerated ash is recovered after being guided to the tank (31), transported to the washing tank (32), put into the washing tank (32), washed with water with a filter press (34), and the solid apatite paste is dried (35). The material dried in the chamber is recovered as a crude apatite, and the alkaline water separated from the filter press (34) is diluted with purified water neutralized by the purifiers (36) and (37) and discharged. The rice bran incinerated ash dehydrated by the filter press is taken out of the rotary kiln (29) and washed with water. The solids of the incinerated ash (38) are transported to the carriage, and the dried apatite enters the drier (39) to the hopper (39 '). What was mixed and kneaded with a filling kneader (40) is pressed into a mold (41), compression molded with a press machine (42), released from the mold, and then fired in a 1000 ° C. firing furnace to produce an artificial bone molding. .
This apatite is processed in the manner of ceramic processing by a conventional method.

The incinerated ash product of processed rice bran obtained by deoiling and fat-degrading this rice bran and removing the agricultural chemical by fermentation is 5% to 8%. When this is further dealkalized, it is reduced to 3%. A product obtained by firing this is apatite, that is, a calcium phosphate salt. This calcium phosphate is free of arsenic sulfur, is harmless as a food, is used as a raw material for baked peking powder, and is optimal as a raw material for artificial teeth and artificial bones.
Since there is no toxic component like general natural phosphate, it is also used as a phosphate raw material for food.

A blending example of the artificial bone is as follows.
Example 4 Rice bran apatite powder 100g
Calcium chloride 5g
120g of water
7g of rice bran powder
1% popal water 5g
Example 5 100g rice ash apatite powder
Alumina chloride 5g
60g of water
Rice bran powder 10g
Magnesium chloride powder 3g
Calcium oxalate 3g
70% 1% popal resin water
Example 3 Apatite powder 100g
3% sodium oxalate 30g
Calcium carbonate 10g
Rice flour 8g
Magnesium oxide 1g
Zirconium oxide 3g
100g of water
Example 4 Apatite gypsum board
100 g of gypsum powder
100g of water
Apatite powder 50g
Calcium biphosphite 10g
30% 5% popal water
Example 4 is an apatite gypsum board used for building material walls and basements. It does not dissolve in water like gypsum.
Example 5 Dried seaweed
Rice bran incineration ash 100g
100g of cultured seaweed (seaweed)
The composition of Example 5 is that the seaweed wakame obtained by marine aquaculture is collected and dried before applying to the rice incineration ash, dried in the sun, washed with water and dried to make a dried wakame.

  FIG. 2 shows a side view of the calcined artificial bone, which is an apatite molded body (joint) (1a) processed from rice bran. This molded body is filled with a mold prepared by adding water to an apatite blend and masticated, and the molded apatite mixture is released and dried in the sun. The dried apatite mixed molded article is guided to a firing furnace and heated at a temperature of 800 to 1200 ° C. for 2 hours, and the apatite molded body (1a) returned to room temperature while being gradually cooled is taken out, and the upper protrusion (2a) of the apatite is removed. A spiral hole is made by drilling in the center with a lathe. Also, the bottom (3a) facing the protrusion is diverged to form porous apatite. This porous apatite is carbonized by the rice bran made into nano-particle powder, and becomes carbon dioxide gas, making many kinds of perforations. Apatite processed with rice bran has a relatively low compressive strength, so it can be improved by adding a reinforcing agent. (4a) shows the joint that becomes the control osteoclast. When the osteoblast fluid (W) is injected into the lower space (5a) of the apatite artificial molded body (1a), the osteoblast grows and expands with time, and the molded body ( The bone formation on the surface of the artificial apatite molded body that substitutes for the spherical bone, which invaded the porous material of 1a), propagated as a dwelling of artificially molded apatite bone, and formed by wearing the broken line at the bottom of the plane of the artificial apatite molded bone into a spherical shape As a circular part.

FIG. 3 shows a side view of an osteoblast and an apatite porous artificial bone, and shows the bone-formed state of FIG. The bottom part of the prosthetic bone of the prosthetic bone that has proliferated is bent and spheroidized, inserted into the perforation (2′a) of the upper protrusion (2a), and screwed with a metal stainless bolt (7a) to be connected to the upper bone.
The increased bone formation (6a) forms a bent bottom surface. (4a) grows so as to embed the space (5a) with artificial proliferative bone in the lower joint bone. (8a) shows the joint.

  FIG. 4 shows a side view of the seabed base of the cultured seaweed.

Rice bran contains 7-8% phosphoric acid and is rich in minerals such as calcium, magnesium, soda and potash. The refined ash of incinerated ash recovered by incineration and washing with water is important as a raw material for artificial bone apatite, and is characterized by high purity and low impurities. The quality is relatively stable. Therefore, the artificial bone formed into a porous shape by using this incinerated ash is used for improving the reinforcement of the inflammatory wear bone of the joint due to osteoporosis. Moreover, when this bone-forming cell solution is impregnated into this porous apatite, it is proliferated and formed on an artificial aggregate to improve the obstacle.
In addition, when artificial bone cells are injected into the body into apatite powder, the bone-affected area is improved by proliferation.
In addition to apatite products used in combination with animal bone powder apatite powder and mixed-firing, it is also used as a building material raw material. In addition, it is used as a rooting base for cultured seaweed such as seaweed. A molded product of a mixture of gypsum and apatite increases productivity, and calcium phosphate is used as a health food for taking.
In addition, to make artificial bone apatite powder and its molded body using rice bran as a raw material, first deoiled oil is extracted to extract 8-10% oil and fat, which is extracted after using it as a raw material for soap, cosmetics and drugs. Incineration ash is made by incineration of the residue. This is used for smoked fish and livestock processing that gradually burns, and the resulting ash is fired and washed with apatite as a raw material for dealkali purification. This can also be used as a dry ash of seaweed as a wrapping agent, and this combination makes it possible to rationalize productivity and further increase added value.

  Process diagram of manufacturing apatite powder fired from rice bran and artificial porous apatite by molding and baking   Side view of molded and fired artificial bone   Side view of a porous artificial bone made of osteoblasts and apatite   Side view of the seabed basement of cultured seaweed

Claims (1)

  The rice bran was defatted, the purified rice bran treated by degreasing by fermentation was separated, and the washed rice was dried and incinerated to recover the ash, which was recovered as apatite powder. An artificial bone powder and an artificial bone ceramic porous molded body and an osteoblastic cell combination are fired by molding.

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