JP2005006566A5 - - Google Patents

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Publication number
JP2005006566A5
JP2005006566A5 JP2003175186A JP2003175186A JP2005006566A5 JP 2005006566 A5 JP2005006566 A5 JP 2005006566A5 JP 2003175186 A JP2003175186 A JP 2003175186A JP 2003175186 A JP2003175186 A JP 2003175186A JP 2005006566 A5 JP2005006566 A5 JP 2005006566A5
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JP
Japan
Prior art keywords
transcription factor
stranded dna
double
mixing
tfiib
Prior art date
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JP2003175186A
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English (en)
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JP2005006566A (ja
JP4274856B2 (ja
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Publication date
Application filed filed Critical
Priority to JP2003175186A priority Critical patent/JP4274856B2/ja
Priority claimed from JP2003175186A external-priority patent/JP4274856B2/ja
Priority to EP04746326A priority patent/EP1650299A4/en
Priority to PCT/JP2004/008857 priority patent/WO2004113529A1/ja
Publication of JP2005006566A publication Critical patent/JP2005006566A/ja
Priority to US11/311,827 priority patent/US7560235B2/en
Publication of JP2005006566A5 publication Critical patent/JP2005006566A5/ja
Application granted granted Critical
Publication of JP4274856B2 publication Critical patent/JP4274856B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Description

【特許請求の範囲】
【請求項１】
蛍光標識された二本鎖ＤＮＡを含む溶液と転写因子ＴＦＩＩＤとを混合するステップと、
前記転写因子ＴＦＩＩＤを混合した溶液に転写因子ＴＦＩＩＢを混合するステップと、
蛍光相関分光法により混合溶液中の蛍光標識を有する物質の並進拡散時間を求めるステップと
を有することを特徴とする転写因子ＴＦＩＩＤと二本鎖ＤＮＡの結合を検出する方法。
【請求項２】
前記転写因子ＴＦＩＩＢを混合するステップの後に、
前記転写因子ＴＦＩＩＢを混合した溶液に抗ＴＦＩＩＢ抗体を混合するステップを含むことを特徴とする請求項１の転写因子ＴＦＩＩＤと二本鎖ＤＮＡの結合を検出する方法。
【請求項３】
前記蛍光相関分光法のコンフォーカルボリューム中における前記蛍光標識された二本鎖ＤＮＡの粒子数が２〜５であることを特徴とする請求項１または２の転写因子ＴＦＩＩＤと二本鎖ＤＮＡの結合を検出する方法。
JP2003175186A 2003-06-19 2003-06-19 Ｄｎａとｄｎａ結合タンパク質との反応を検出する方法 Expired - Fee Related JP4274856B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2003175186A JP4274856B2 (ja) 2003-06-19 2003-06-19 Ｄｎａとｄｎａ結合タンパク質との反応を検出する方法
EP04746326A EP1650299A4 (en) 2003-06-19 2004-06-17 METHOD FOR DETECTING A REACTION BETWEEN DNA AND DNA BINDING PROTEINS
PCT/JP2004/008857 WO2004113529A1 (ja) 2003-06-19 2004-06-17 Ｄｎａとｄｎａ結合タンパク質との反応を検出する方法
US11/311,827 US7560235B2 (en) 2003-06-19 2005-12-19 Method of detecting reaction of DNA and DNA-binding protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2003175186A JP4274856B2 (ja) 2003-06-19 2003-06-19 Ｄｎａとｄｎａ結合タンパク質との反応を検出する方法

Publications (3)

Publication Number Publication Date
JP2005006566A JP2005006566A (ja) 2005-01-13
JP2005006566A5 true JP2005006566A5 (ja) 2006-03-23
JP4274856B2 JP4274856B2 (ja) 2009-06-10

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ID=33534812

Family Applications (1)

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JP2003175186A Expired - Fee Related JP4274856B2 (ja) 2003-06-19 2003-06-19 Ｄｎａとｄｎａ結合タンパク質との反応を検出する方法

Country Status (4)

Country Link
US (1) US7560235B2 (ja)
EP (1) EP1650299A4 (ja)
JP (1) JP4274856B2 (ja)
WO (1) WO2004113529A1 (ja)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4338590B2 (ja) * 2004-06-03 2009-10-07 独立行政法人科学技術振興機構 蛍光相関分光法による抗原の迅速検出及び／又は測定法。
JP2009278866A (ja) * 2006-09-04 2009-12-03 Olympus Corp Ｄｎａに於けるｄｎａ結合性タンパク質の結合部位の検出方法
JP2009008608A (ja) * 2007-06-29 2009-01-15 Sysmex Corp Ｄｎａ結合タンパクの定量方法及び定量用キット
CN103901006B (zh) * 2014-03-26 2016-04-13 华南师范大学 基于ZnO量子点检测镉离子的试剂及方法
CN116391042A (zh) * 2020-10-02 2023-07-04 新加坡国立大学 Dna组装混合物和其使用方法
WO2023039491A2 (en) * 2021-09-09 2023-03-16 Proof Diagnostics, Inc. Coronavirus rapid diagnostics

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5804374A (en) * 1980-12-05 1998-09-08 Massachusetts Insti. Technology Nuclear factors associates with transcriptional regulation
AU658257B2 (en) * 1991-10-31 1995-04-06 Matritech, Inc. Nuclear matrix protein fluid assay
DE19533092A1 (de) * 1995-09-07 1997-03-13 Basf Ag Vorrichtung zur parallelisierten Zweiphotonen-Fluoreszenz-Korrelations-Spektroskopie (TPA-FCS) und deren Verwendung zum Wirkstoff-Screening
GB0120441D0 (en) * 2001-08-22 2001-10-17 Novartis Forschungsstiftung Model of autoimmune disease and methods for identifying anti autoimmune disease disorders

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