JP2004515248A5 - - Google Patents
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- Publication number
- JP2004515248A5 JP2004515248A5 JP2002550068A JP2002550068A JP2004515248A5 JP 2004515248 A5 JP2004515248 A5 JP 2004515248A5 JP 2002550068 A JP2002550068 A JP 2002550068A JP 2002550068 A JP2002550068 A JP 2002550068A JP 2004515248 A5 JP2004515248 A5 JP 2004515248A5
- Authority
- JP
- Japan
- Prior art keywords
- polynucleotide
- exonuclease
- population
- parent
- strand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 description 15
- 108091033319 polynucleotide Proteins 0.000 description 15
- 102000040430 polynucleotide Human genes 0.000 description 15
- 239000002157 polynucleotide Substances 0.000 description 15
- 239000012634 fragment Substances 0.000 description 8
- 108060002716 Exonuclease Proteins 0.000 description 5
- 102000013165 exonuclease Human genes 0.000 description 5
- 238000002703 mutagenesis Methods 0.000 description 3
- 231100000350 mutagenesis Toxicity 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- -1 BAL31 Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010007577 Exodeoxyribonuclease I Proteins 0.000 description 1
- 108010046914 Exodeoxyribonuclease V Proteins 0.000 description 1
- 102100029075 Exonuclease 1 Human genes 0.000 description 1
- 102000019236 Exonuclease V Human genes 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0030252A GB2370038B (en) | 2000-12-12 | 2000-12-12 | A method for in vitro molecular evolution of protein function |
| US09/734,801 US6958213B2 (en) | 2000-12-12 | 2000-12-12 | Method for in vitro molecular evolution of protein function |
| PCT/EP2001/014744 WO2002048351A2 (en) | 2000-12-12 | 2001-12-12 | A method for in vitro molecular evolution of protein function |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004515248A JP2004515248A (ja) | 2004-05-27 |
| JP2004515248A5 true JP2004515248A5 (https=) | 2009-01-22 |
| JP4430303B2 JP4430303B2 (ja) | 2010-03-10 |
Family
ID=26245418
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002550068A Expired - Fee Related JP4430303B2 (ja) | 2000-12-12 | 2001-12-12 | タンパク質機能のインビトロ分子進化方法 |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP1341909B2 (https=) |
| JP (1) | JP4430303B2 (https=) |
| AT (1) | ATE295882T1 (https=) |
| AU (2) | AU2002234572B2 (https=) |
| CA (1) | CA2430582C (https=) |
| DE (1) | DE60110926T3 (https=) |
| DK (1) | DK200300821A (https=) |
| ES (1) | ES2244670T5 (https=) |
| PT (1) | PT1341909E (https=) |
| WO (1) | WO2002048351A2 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7153655B2 (en) | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
| US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
| US20030036641A1 (en) * | 2001-01-31 | 2003-02-20 | Padgett Hal S. | Methods for homology-driven reassembly of nucleic acid sequences |
| DK1504098T4 (da) * | 2002-05-17 | 2011-05-23 | Alligator Bioscience Ab | Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion |
| WO2004016811A2 (en) * | 2002-08-19 | 2004-02-26 | Danmarks Tekniske Universitet (Dtu) | Methods and kit for genes shuffling using tagged primers |
| GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
| GB0607798D0 (en) | 2006-04-20 | 2006-05-31 | Alligator Bioscience Ab | Novel polypeptides and use thereof |
| GB0708376D0 (en) | 2007-05-01 | 2007-06-06 | Alligator Bioscience Ab | Novel polypeptides and uses thereof |
| US8383346B2 (en) | 2008-06-13 | 2013-02-26 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
| GB0905790D0 (en) | 2009-04-03 | 2009-05-20 | Alligator Bioscience Ab | Novel polypeptides and use thereof |
| GB0920258D0 (en) | 2009-11-19 | 2010-01-06 | Alligator Bioscience Ab | New medical agents and use thereof |
| GB201115280D0 (en) | 2011-09-05 | 2011-10-19 | Alligator Bioscience Ab | Antibodies, uses and methods |
| GB201311475D0 (en) | 2013-06-27 | 2013-08-14 | Alligator Bioscience Ab | Polypeptides |
| GB201311487D0 (en) | 2013-06-27 | 2013-08-14 | Alligator Bioscience Ab | Bispecific molecules |
| CA2957146A1 (en) | 2014-08-12 | 2016-02-18 | Alligator Bioscience Ab | Combination therapies with anti cd40 antibodies |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5851804A (en) * | 1996-05-06 | 1998-12-22 | Apollon, Inc. | Chimeric kanamycin resistance gene |
| GB9712512D0 (en) * | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
| WO2000072013A1 (en) * | 1999-05-21 | 2000-11-30 | The Penn State Research Foundation | Construction of incremental truncation libraries |
-
2001
- 2001-12-12 EP EP01985402A patent/EP1341909B2/en not_active Expired - Lifetime
- 2001-12-12 WO PCT/EP2001/014744 patent/WO2002048351A2/en not_active Ceased
- 2001-12-12 AU AU2002234572A patent/AU2002234572B2/en not_active Ceased
- 2001-12-12 ES ES01985402T patent/ES2244670T5/es not_active Expired - Lifetime
- 2001-12-12 AT AT01985402T patent/ATE295882T1/de active
- 2001-12-12 CA CA2430582A patent/CA2430582C/en not_active Expired - Fee Related
- 2001-12-12 PT PT01985402T patent/PT1341909E/pt unknown
- 2001-12-12 AU AU3457202A patent/AU3457202A/xx active Pending
- 2001-12-12 DE DE60110926T patent/DE60110926T3/de not_active Expired - Lifetime
- 2001-12-12 JP JP2002550068A patent/JP4430303B2/ja not_active Expired - Fee Related
-
2003
- 2003-06-03 DK DK200300821A patent/DK200300821A/da not_active Application Discontinuation
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