JP2004229598A - Method for acclimating and transplanting seedling - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To particularly provide a method suitable for acclimation and transplantation of strawberry seedlings, in a method for acclimating and transplanting seedlings in which cultured in vitro seedlings are acclimated and then, transplanted into a culture vessel such as a pot. <P>SOLUTION: In vitro seedling is cultured in a culture vessel and the in vitro seedling is taken out from the culture vessel in a state in which the root is attached to the seedling and transferred into a hydroponic apparatus and the transferred in vitro seedling is acclimated while moistening the seedling by covering the seedling with a sheet and the lower part of root of the acclimated in vitro seedling is cut to separate the in vitro seedling and the separated in vitro seedling is transplanted into a culture (seedling-raising) vessel. <P>COPYRIGHT: (C)2004,JPO&NCIPI

Description

[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a method of acclimating and transplanting seedlings that are cultured and then transplanted into a cultivation container such as a pot after acclimating the cultured in vitro seedlings, and particularly a method suitable for acclimating and transplanting strawberry seedlings.
[0002]
[Prior art]
Generally, the culture environment of in vitro seedlings is aseptic and humid than the natural environment, and thus the cultured in vitro seedlings have low resistance to bacteria and weather. Therefore, the in vitro seedlings are grown in a natural environment after acclimation so as to have fungi resistance and weather resistance. The cultured in vitro seedlings are taken out of the culture container together with the roots, divided into strains, transplanted into cultivation containers such as seedling raising trays and seedling raising pots, and acclimated in an acclimatized environment.
[0003]
A culture vessel for culturing strawberry seedlings has an upper opening, and a wire mesh is set detachably at a constricted portion in the depth direction, and a culture solution can be stored below the wire mesh. When strawberry seedlings are cultured using this type of culture container, a plurality of shoots containing the growing points of the strawberry seedlings are placed on the wire net and cultured. The cultured in vitro seedlings are taken out of the culture vessel together with the wire netting, divided into strains, transplanted into a cultivation vessel such as a nursery tray or a nursery pot, and allowed to acclimate in an acclimatized environment for about one week. See Patent Document 1 for details.
[0004]
[Patent Document 1]
JP-A-9-220022 [0005]
[Problems to be solved by the invention]
The conventional methods for acclimatizing and transplanting strawberry seedlings described above have the following problems.
(1) Since the roots of the in vitro seedlings are excised during transplantation to seedling pots or the like, the seedlings are weakened, stress is applied to the seedlings, and the subsequent seedlings grow slowly or small seedlings can grow. Sometimes they die.
(2) In order to solve the above-mentioned problems, it is conceivable to grow the in vitro seedlings in a culture vessel by lengthening the culture period, but the culture vessel is too small to grow to the extent that the problem does not occur. . Even if the volume of the culture vessel is increased, the inside of the culture vessel is not suitable for growth in terms of air permeability and other aspects.
(3) Mist must be sprayed to maintain humidity during acclimatization, and it takes time and effort to manage and maintain the acclimatization environment, resulting in an increase in cost.
(4) The separated strawberry seedlings have thin and soft stems and roots, so they are difficult to handle and require a lot of labor for separation.
(5) Even if separated, immature seedlings that are not suitable for transplantation cannot be transplanted, so that the number of in vitro seedlings that can be transplanted is small.
[0006]
[Means for Solving the Problems]
An object of the present invention is to provide a method for acclimating and transplanting seedlings, which solves the above-mentioned problems.
[0007]
The method for acclimating / transplanting seedlings according to claim 1 of the present invention is a method for acclimating / transplanting in vitro seedlings cultured in a culture vessel and transplanting them into a cultivation vessel such as a pot. The in vitro seedlings are taken out of the culture vessel with the roots attached, transferred to a hydroponic cultivation device, covered with a sheet and acclimated, a number of acclimated seedlings are separated, and each separated seedling is cultivated in a pot or the like. It is a method characterized by transplanting into a container.
[0008]
In the method for acclimating and transplanting seedlings according to claim 2 of the present invention, in the method for acclimating and transplanting seedlings described above, an in vitro seedling cultured on a net set in a culture vessel is taken out together with the net. It is a method characterized by transferring to a hydroponic cultivation apparatus.
[0009]
The method for acclimating and transplanting seedlings according to claim 3 of the present invention is characterized in that, in the method for acclimating and transplanting seedlings described above, the seedlings separated after the acclimation are transplanted into a cultivation container connected in a number of rows and columns. Is a method.
[0010]
BEST MODE FOR CARRYING OUT THE INVENTION
(Embodiment 1)
An embodiment of the present invention will be described with reference to the drawings. This embodiment is an example of a case where a cultured strawberry seedling is adapted and transplanted. In this embodiment, a number of strawberry shoots are cultivated in a culture vessel 1 having a relative humidity of about 100% (at least 80% or more) and a temperature of 23 to 25 ° C. to obtain in vitro seedlings 5. The number of the in vitro seedlings 5 varies depending on the size of the culture vessel 1, but is usually 270 to 280. The culture vessel 1 is a transparent vessel with a bottom provided with a lid 2 and has a constricted portion 3 in the middle in the depth direction. The constricted portion 3 can be set by locking a wire net 4. In order to culture a strawberry in vitro seedling 5 as shown in FIG. 1 (b), as shown in FIG. 1 (a), a culture solution is put into a culture vessel 1 to form a liquid medium 6, and A plurality of shoots 7 containing growing points taken out from a strawberry runner are placed on the wire mesh 4 and cultured. At this time, the liquid level of the liquid medium 6 is set to be lower than the wire net 4 so that the chute 7 can contact both gas and liquid. The wire net 4 can be replaced with a net made of synthetic resin, a metal plate provided with a large number of through holes, a plastic plate, or the like. In short, any type can be used as long as the chute 7 in the culture vessel 1 can be supported so as to be in contact with both gas and liquid. The liquid medium can be replaced with an agar medium.
[0011]
Next, the in vitro seedlings 5 in the culture vessel 1 are taken out together with the wire netting 4 while keeping the roots, and the procedure is performed. The acclimation is performed using a hydroponic cultivation apparatus (for example, Mikado hydroponic cultivation apparatus). Specifically, the in vitro seedling 5 is inserted (set) together with the wire netting 4 into a hole 9 provided in a lid 10 on a hydroponic culture bed 8 as shown in FIG. At this time, it is desirable that the diameter of the hole 9 be slightly smaller than the diameter of the wire mesh 4 so that the outer peripheral portion of the wire mesh 4 is locked to the peripheral edge of the hole 9 so as not to drop. Also, instead of setting the in vitro seedlings 5 in all the holes 9 of the lid 10, it is not necessary to set them in some of the holes 9, and the holes are left open to ensure air permeability into the bed. Is preferable for the growth of seedlings. During acclimation by the hydroponic cultivation apparatus, the in vitro seedling 5 is moisturized by covering the hydroponic cultivation bed 8 with the sheet 13 as shown in FIG. Although the acclimatization period varies depending on the acclimatization environment in the sheet 13, it is generally preferable that the acclimation environment be performed at a humidity of 60 to 80% and a temperature of 15 to 25 ° C. for 7 to 8 days. If the temperature is too low, a low temperature fault will occur, and if it is too high, a high temperature fault will occur. It is desirable to use a sheet having a high oblique rate when the outside air temperature is high, and to maintain the temperature as low as possible using a sheet having a low oblique rate when the outside air temperature is low. Two or more sheets may be covered at an interval above and below. In that case, the same type or different types of sheets can be used. The sheet is suitably made of resin.
[0012]
The in vitro seedlings 5 that have been acclimated by the hydroponic cultivation apparatus are removed together with the wire mesh 4 from the hydroponic cultivation bed 8, and the lower part of the root 11 of the in vitro seedling 5a protruding below the wire mesh 4 is removed as shown in FIG. Excision is performed and the in vitro seedlings 5 are separated into individual seedlings 5a.
[0013]
Each of the separated strawberry seedlings 5a is transplanted to a cultivation (seedling) container 12 such as a pot or a plug tray as shown in FIG. Although the cultivation container 12 shown in FIG. 5 is a single seedling raising pot, the strawberry seedling 5a can also be transplanted to a connection pot in which many pots are connected vertically and horizontally, for example, a plug tray (cell tray) or the like. The present invention can be used not only for strawberry seedlings but also for acclimation and transplantation of seedlings of other plants.
[0014]
【The invention's effect】
According to the present invention, the in vitro seedlings are acclimated using the hydroponic cultivation apparatus, and are grown and grown sufficiently before separation and transplantation.
(1) Since the roots are cut after acclimating and sufficiently growing the in vitro seedlings, the stress applied to the seedlings by cutting the roots is reduced, and there is little hindrance to the subsequent growth.
(2) Since the hydroponic cultivation apparatus is used for acclimatization, the trouble of spraying mist as in the related art is unnecessary, and the trouble and cost for acclimation are reduced.
(3) By acclimatization using the hydroponic cultivation device, the stems and roots grow sufficiently, become thicker and stronger, and then the seedlings are separated. Time is also reduced. In addition, waste of seedlings is reduced, and the number of seedlings that can be taken out of in vitro seedlings cultured in one culture vessel increases (yield improves).
Specifically, in the conventional method, about 120 seedlings can be obtained from in vitro seedlings cultured in one culture vessel, but according to the present invention, about 200 seedlings can be obtained.
[Brief description of the drawings]
FIGS. 1A and 1B are explanatory diagrams showing a cultivation process of a strawberry seedling in a cultivation container.
FIG. 2 is an explanatory diagram of a step of acclimating a strawberry seedling with a hydroponic cultivation apparatus.
FIG. 3 is an explanatory view showing a state in which a hydroponic cultivation apparatus for acclimating strawberry seedlings is covered with a sheet.
FIG. 4 is an explanatory diagram showing the separated state of acclimated in vitro seedlings.
FIG. 5 is an explanatory view showing a state where the separated strawberry seedlings are transplanted into a cultivation container.
[Explanation of symbols]
REFERENCE SIGNS LIST 1 culture vessel 2 culture vessel lid 3 culture vessel neck 4 wire mesh 5 in vitro seedling 6 liquid medium 7 shoot 8 hydroponic bed 9 hydroponic bed hole 10 hydroponic bed lid 11 seedling root 13 sheet

Claims (3)

A method of acclimating and transplanting an in vitro seedling cultured in a culture container and transplanting the plant into a cultivation container such as a pot. The seedlings are acclimated and transplanted by covering the seedlings with sheets and acclimating the seedlings, separating a large number of the acclimated seedlings, and transplanting the separated seedlings into cultivation containers such as pots. Method. 2. The method of acclimating and transplanting seedlings according to claim 1, wherein the in vitro seedlings cultured on the net set in the culture vessel are taken out and transferred to a hydroponic cultivation apparatus. 3. The method for acclimating and transplanting seedlings according to claim 1 or 2, wherein the seedlings separated after acclimation are transplanted into a cultivation container connected in a number of rows and columns.

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