CN215188666U - Device is tamed to melon interspecific hybrid tissue culture seedling - Google Patents

Abstract

The utility model discloses a melon interspecific hybrid tissue culture seedling domestication device, including moisturizing printing opacity cover, nutrient solution cup and matrix container, the bottom opening of moisturizing printing opacity cover, the open-top of nutrient solution cup, the matrix container is uncovered container, the upper portion periphery of matrix container is equipped with the support flange, the support flange support in the opening of nutrient solution cup, matrix container part set up in within the nutrient solution cup, the uncovered of matrix container is equipped with the adjustable ring of inside undergauge, the adjustable ring is equipped with a plurality of through-holes, the bottom of matrix container be equipped with by the inside downwardly extending's of matrix container siphon strip, the bottom opening of moisturizing printing opacity cover with the interior joint of following of adjustable ring. The utility model discloses the structure controllability is stronger, and the structure is light and handy, and easy operation is applicable to the domestication of the interspecific hybrid tissue culture seedling that quantity is rare, the growth vigor is weak, difficult to survive.

Description

Device is tamed to melon interspecific hybrid tissue culture seedling

Technical Field

The utility model relates to a plant seedling domesticates device, especially a device is domesticated to melon interspecific hybrid tissue culture seedling.

Background

The melon belongs to Cucumis melo species (Cucumis melo.L.) in Cucumis of Cucurbitaceae (Cucumis Juss), and is an annual trailing sprawl herb. With the improvement of living standard, people have higher requirements on the quality of melons. Genetic variation is the basis of variety improvement, however, breeding practice shows that intraspecific variation sometimes cannot provide disease resistance or stress resistance which is urgently needed by cultivated varieties. Interspecific hybridization is an important way for discovering excellent genes utilizing wild germplasm resources, possibly realizing advantage complementation and improving the heredity and the variety of melon crops. The interspecific hybridization of cucumis plants is difficult, at present, only the professor team of chenjinfeng in China adopts embryo rescue and chromosome doubling fertility restoring technology to successfully perform interspecific hybridization of common cultivated cucumber (2n ═ 14) and wild cucumber C.hystrix (2n ═ 24) of the same genus, and precious interspecific hybrid resources are obtained.

However, since interspecific hybrid tissue culture seedlings are not well differentiated due to the incompatibility of the amphiphilic genetic materials, and are easy to yellow and thin, young tissue culture seedlings are fragile, weak in photosynthetic capacity and poor in adaptability. If the tissue culture seedlings are planted in the field by a conventional domestication and transplantation mode, the survival rate of the seedlings is extremely low. The common tissue culture seedling transplanting and domesticating technology for scientific research at present is to transplant tissue culture seedlings into a glass greenhouse controlled by human environment, is suitable for more groups, and is relatively wasted when used for interspecific hybrid materials with small quantity. If the problem of domestication of the interspecific hybrid tissue culture seedlings cannot be solved well, a novel interspecific hybrid germplasm cannot be obtained.

SUMMERY OF THE UTILITY MODEL

To the above-mentioned prior art defect, the utility model aims to provide a device is tamed to melon interspecific hybrid tissue culture seedling to solve the problem that operation is complicated, the survival rate is low is tamed and transplanted to interspecific hybrid tissue culture seedling that quantity is rare.

The utility model discloses technical scheme as follows: the utility model provides a melon interspecific hybrid tissue culture seedling domestication device, includes moisturizing printing opacity cover, nutrient solution cup and matrix container, the bottom opening of moisturizing printing opacity cover, the open-top of nutrient solution cup, the matrix container is uncovered container, the upper portion periphery of matrix container is equipped with the support flange, the support flange support in the opening of nutrient solution cup, matrix container part set up in within the nutrient solution cup, the uncovered of matrix container is equipped with the adjustable ring of inside undergauge, the adjustable ring is equipped with a plurality of through-holes, the bottom of matrix container be equipped with by the inside downwardly extending's of matrix container siphon strip, the bottom opening of moisturizing printing opacity cover with along pegging graft in the adjustable ring.

Furthermore, in order to facilitate the adjustment of the humidity of the matrix and the gas exchange between the tissue culture seedling and the external environment, the through hole of the adjusting ring is provided with an opening and closing mechanism, and the opening and closing mechanism is used for adjusting the size of the through hole.

Furthermore, the opening and closing mechanism comprises a rotating ring which is overlapped on the adjusting ring, the rotating ring is provided with a plurality of adjusting holes, and the rotating ring is sleeved outside the moisturizing light-transmitting cover.

Furthermore, in order to facilitate the assembly of the moisturizing light-transmitting cover, an upward annular convex edge is arranged on the inner edge of the adjusting ring, an opening at the bottom of the moisturizing light-transmitting cover is inserted into the annular convex edge, and the rotating ring is sleeved outside the annular convex edge.

Further, the diameter of the through hole is 4-5 mm.

Further, the height of the moisturizing light-transmitting cover is 12-15 cm.

Further, the diameter of the nutrient solution cup body is 12-15 cm, and the diameter of the moisturizing light-transmitting cover is 8-10 cm.

Compared with the prior art, the utility model the advantage lie in:

the utility model discloses a tissue culture seedling transplants and provides the domestication environment of a gradual adaptation, smelts seedling earlier stage, and the tissue culture seedling plant is tender, fragile, and the root differentiation is incomplete, and the autotrophy ability is weaker, and the printing opacity cover of moisturizing keeps the plant not direct contact with external environment, lets its gradual adaptation, and the adjustable ring through the matrix container simultaneously provides certain humidity for the seedling, prevents that the seedling from withering and withering because of the dehydration, and can guarantee that the root has sufficient oxygen, promotes the tissue culture seedling and sends out new roots. When the plant growth condition is stable, the moisture-preserving light-transmitting cover can be easily taken down, the plant is directly exposed in the external environment, and when the climatic condition is appropriate, the seedling is transplanted to the field.

The utility model discloses a domestication device controllability is stronger, and the structure is light and handy, and easy operation is applicable to the domestication of the interspecific hybrid tissue culture seedling that quantity is rare, the growth vigor is weak, difficult survival.

Drawings

FIG. 1 is a schematic structural view of a device for domesticating tissue culture seedlings of interspecific hybrids of melons in example 1.

Fig. 2 is a schematic top view of the substrate container of the device for acclimatizing tissue culture seedlings of interspecific hybrids of melons in example 1.

FIG. 3 is a schematic structural view of the device for domesticating tissue culture seedlings of interspecific hybrids of melons in example 2.

Detailed Description

The present invention will be further described with reference to the following examples, which should not be construed as limiting the invention.

In embodiment 1, please refer to fig. 1 and 2, the domestication device for tissue culture seedlings of interspecific hybrids of melons in this embodiment includes a moisturizing light-transmitting cover 1, a nutrient solution cup body 2 and a substrate container 3, wherein the moisturizing light-transmitting cover 1 is made of transparent plastic, the bottom opening of the moisturizing light-transmitting cover is entirely columnar, the top of the moisturizing light-transmitting cover 1 is hemispherical, the height of the moisturizing light-transmitting cover is 12-15 cm, and the diameter of the moisturizing light-transmitting cover is 8-10 cm, so as to provide a moisturizing light-transmitting growth space for the plant domestication process. Nutrient solution cup 2 and matrix container 3 are the intussusception setting, and the diameter of nutrient solution cup 2 is 12 ~ 15cm, the open-top of nutrient solution cup 2. The substrate container 3 is made of opaque plastic material and is a semi-ellipsoidal open container. The upper periphery of the substrate container 3 is provided with a supporting flange 31, the supporting flange 31 is supported on the opening of the nutrient solution cup body 2, so that the substrate container 3 is partially arranged in the nutrient solution cup body 2, and a sufficient gap is left between the bottom of the substrate container 3 and the bottom of the nutrient solution cup body 2 to contain nutrient solution. The substrate container 3 is used for bearing culture substrates, and in order to ensure that the substrates can continuously supplement nutrient elements for plant growth, the bottom of the substrate container 3 is provided with a siphon strip 4 which extends downwards from the inside of the substrate container 3. After nutrient solution is added into the nutrient solution cup body 2, the substrate can absorb the nutrient solution through the siphon strips 4. The structure that nutrient solution cup 2 and matrix container 3 separate can conveniently in time add nutrient solution to keep the clean and tidy of nutrient solution.

An opening of the substrate container 3 is provided with an adjusting ring 32 which is inwardly reduced in diameter, the adjusting ring 32 is provided with a plurality of through holes 33 with the diameter of 4-5 mm, and the bottom opening of the moisturizing light-transmitting cover 1 is inserted into the inner edge of the adjusting ring 32, so that a relatively sealed growth space is formed between the moisturizing light-transmitting cover 1 and the substrate container 3, the environment for growing the tissue culture seedlings is guaranteed to have certain humidity, and the plants are prevented from wilting due to excessive water loss. And the through hole 33 is used as a gas and humidity adjusting structure, so that gas exchange of the tissue culture seedlings due to respiration is facilitated, and meanwhile, the growth environment of the hybrid plants can be prevented from being too humid.

The operation steps of domesticating by using the muskmelon interspecific hybrid tissue culture seedling domestication device are as follows:

1) placing the matrix, knife forceps and dish in a high temperature sterilizing pot for high temperature sterilization, placing in a super clean bench together with sterile water, nutrient solution and washed container, and irradiating with ultraviolet lamp for 30 min;

2) filling a part of sterilized matrix in a matrix container 3, taking out the tissue culture seedlings from a tissue culture bottle by using fired and cooled knife forceps, putting the tissue culture seedlings in sterile water to clean plant root systems, clamping the plants in a culture structure by using big forceps, filling the matrix again, compacting the matrix, and pouring a proper amount of nutrient solution to promote the matrix and the root to be tightly attached together so as to facilitate the growth of the plants;

3) a certain amount of nutrient solution is added into the nutrient solution cup body 2 for the growth of plants, and when the plants are domesticated for the first time, the nutrient solution needs to be sufficient, but not too much;

4) combining the substrate container 3 and the nutrient solution cup body 2 to ensure that the siphon strips 4 can absorb the nutrient solution in the nutrient solution cup body 2, and finally buckling the moisturizing light-transmitting cover 1 on the substrate container 3;

5) when the plants can grow normally and robustly, the structure of the moisturizing light-transmitting cover 1 is removed, and the plants are transplanted when growing to be transplanted.

In embodiment 2, referring to fig. 3, the domestication device for tissue culture seedlings of interspecific hybrids of melons in this embodiment includes a moisturizing light-transmitting cover 1, a nutrient solution cup body 2 and a substrate container 3, wherein the moisturizing light-transmitting cover 1 is made of transparent plastic, an opening at the bottom of the moisturizing light-transmitting cover is integrally columnar, and the top of the moisturizing light-transmitting cover 1 is hemispherical, 12-15 cm in height and 8-10 cm in diameter. Nutrient solution cup 2 and matrix container 3 are the intussusception setting, and the diameter of nutrient solution cup 2 is 12 ~ 15cm, the open-top of nutrient solution cup 2. The substrate container 3 is made of opaque plastic material and is a semi-ellipsoidal open container. The upper periphery of the substrate container 3 is provided with a supporting flange 31, the supporting flange 31 is supported on the opening of the nutrient solution cup body 2, so that the substrate container 3 is partially arranged in the nutrient solution cup body 2, and a sufficient gap is left between the bottom of the substrate container 3 and the bottom of the nutrient solution cup body 2 to contain nutrient solution. The bottom of the substrate container 3 is provided with a wick 4 extending downwardly from the interior of the substrate container 3. The uncovered adjustable ring 32 that is equipped with inside undergauge of matrix container 3, adjustable ring 32 is equipped with a plurality of diameters and is 4 ~ 5mm through-hole 33, the interior edge of adjustable ring 32 is equipped with ascending annular protruding edge 34, the bottom opening of the printing opacity cover 1 of moisturizing is pegged graft in annular protruding edge 34, set up 5 covers of swivel that a plurality of diameters are 4 ~ 5 mm's regulation hole 51 and locate outside annular protruding edge 34, change the ventilative and effect of loosing moisture of matrix just can be adjusted as required to the degree of coincidence of through-hole 33 and regulation hole 51 through rotating swivel 5, in order to satisfy the requirement of plant domestication.

Claims (7)

1. The utility model provides a melon interspecific hybrid tissue culture seedling domestication device, its characterized in that, is including moisturizing printing opacity cover, nutrient solution cup and matrix container, the bottom opening of moisturizing printing opacity cover, the open-top of nutrient solution cup, the matrix container is uncovered container, the upper portion periphery of matrix container is equipped with the support flange, the support flange support in the opening of nutrient solution cup, matrix container part set up in within the nutrient solution cup, the uncovered of matrix container is equipped with the adjustable ring of inside undergauge, the adjustable ring is equipped with a plurality of through-holes, the bottom of matrix container be equipped with by the inside downwardly extending's of matrix container siphon strip, the bottom opening of moisturizing printing opacity cover with the interior joint of following of adjustable ring.

2. The domestication device for tissue culture seedlings of interspecific hybrids of melons according to claim 1, wherein the through hole of the adjusting ring is provided with an opening and closing mechanism for adjusting the size of the through hole.

3. The domestication device for tissue culture seedlings of interspecific hybrids of melons according to claim 2, wherein the opening and closing mechanism comprises a rotating ring overlapped on the adjusting ring, the rotating ring is provided with a plurality of adjusting holes, and the rotating ring is sleeved outside the moisture-preserving light-transmitting cover.

4. The domestication device for tissue culture seedlings of interspecific hybrids of melons according to claim 3, wherein an upward annular convex edge is arranged on an inner edge of the adjusting ring, a bottom opening of the moisturizing light-transmitting cover is inserted into the annular convex edge, and the rotating ring is sleeved outside the annular convex edge.

5. The domestication device for tissue culture seedlings of interspecific hybrids of melons according to claim 1, wherein the diameter of the through hole is 4-5 mm.

6. The acclimatization device for the tissue culture seedlings of the interspecific hybrids of melons according to claim 1, wherein the height of the moisture-preserving light-transmitting cover is 12-15 cm.

7. The domestication device for tissue culture seedlings of melons interspecies hybrids according to claim 1, wherein the diameter of the nutrient solution cup body is 12-15 cm, and the diameter of the moisturizing light-transmitting cover is 8-10 cm.

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