JP2004212355A - バイオ電子顕微鏡及び試料の観察方法 - Google Patents

バイオ電子顕微鏡及び試料の観察方法 Download PDF

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Publication number
JP2004212355A
JP2004212355A JP2003002724A JP2003002724A JP2004212355A JP 2004212355 A JP2004212355 A JP 2004212355A JP 2003002724 A JP2003002724 A JP 2003002724A JP 2003002724 A JP2003002724 A JP 2003002724A JP 2004212355 A JP2004212355 A JP 2004212355A
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JP
Japan
Prior art keywords
sample
image
electron beam
electron microscope
acceleration voltage
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Pending
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JP2003002724A
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English (en)
Japanese (ja)
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JP2004212355A5 (enExample
Inventor
Hiroshi Kakibayashi
博司 柿林
Shigeyuki Hosoki
茂行 細木
Yuji Takagi
裕治 高木
Akira Miyake
亮 三宅
Kuniyasu Nakamura
邦康 中村
Mitsugi Sato
佐藤　　貢
Hiroyuki Kobayashi
弘幸 小林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Hitachi High Tech Corp
Original Assignee
Hitachi High Technologies Corp
Hitachi Ltd
Hitachi High Tech Corp
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Publication date
Application filed by Hitachi High Technologies Corp, Hitachi Ltd, Hitachi High Tech Corp filed Critical Hitachi High Technologies Corp
Priority to JP2003002724A priority Critical patent/JP2004212355A/ja
Priority to EP20030016333 priority patent/EP1437758A2/en
Priority to US10/621,444 priority patent/US6875984B2/en
Publication of JP2004212355A publication Critical patent/JP2004212355A/ja
Publication of JP2004212355A5 publication Critical patent/JP2004212355A5/ja
Pending legal-status Critical Current

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    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/02—Details
    • H01J37/22—Optical, image processing or photographic arrangements associated with the tube
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/26—Electron or ion microscopes; Electron or ion diffraction tubes
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J2237/00—Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
    • H01J2237/05—Arrangements for energy or mass analysis
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J2237/00—Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
    • H01J2237/26—Electron or ion microscopes
    • H01J2237/28—Scanning microscopes
    • H01J2237/2809—Scanning microscopes characterised by the imaging problems involved
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J2237/00—Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
    • H01J2237/26—Electron or ion microscopes
    • H01J2237/28—Scanning microscopes
    • H01J2237/2813—Scanning microscopes characterised by the application

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
JP2003002724A 2003-01-09 2003-01-09 バイオ電子顕微鏡及び試料の観察方法 Pending JP2004212355A (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2003002724A JP2004212355A (ja) 2003-01-09 2003-01-09 バイオ電子顕微鏡及び試料の観察方法
EP20030016333 EP1437758A2 (en) 2003-01-09 2003-07-18 Electron microscope and observation method of biological specimen
US10/621,444 US6875984B2 (en) 2003-01-09 2003-07-18 Bio electron microscope and observation method of specimen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2003002724A JP2004212355A (ja) 2003-01-09 2003-01-09 バイオ電子顕微鏡及び試料の観察方法

Publications (2)

Publication Number Publication Date
JP2004212355A true JP2004212355A (ja) 2004-07-29
JP2004212355A5 JP2004212355A5 (enExample) 2006-01-19

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JP2003002724A Pending JP2004212355A (ja) 2003-01-09 2003-01-09 バイオ電子顕微鏡及び試料の観察方法

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US (1) US6875984B2 (enExample)
EP (1) EP1437758A2 (enExample)
JP (1) JP2004212355A (enExample)

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JP2008032531A (ja) * 2006-07-28 2008-02-14 Kao Corp 細菌の共凝集能の評価方法
JP2013541718A (ja) * 2010-10-29 2013-11-14 サーモ・フィッシャー・サイエンティフィック・オイ サンプルの準備および分析のための自動化されたシステム
JP2014157772A (ja) * 2013-02-18 2014-08-28 Horon:Kk 走査型電子顕微鏡および検査装置
JP2019204695A (ja) * 2018-05-24 2019-11-28 日本電子株式会社 生物組織画像処理装置及び方法
WO2019225506A1 (ja) * 2018-05-24 2019-11-28 株式会社ニコン 生物組織画像処理システム及び機械学習方法
CN111065950A (zh) * 2017-09-15 2020-04-24 敏捷焦点设计有限责任公司 用于宽场、共焦和多光子显微镜的动态聚焦和变焦系统

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JP4154300B2 (ja) * 2003-09-08 2008-09-24 株式会社日立ハイテクノロジーズ 透過電子顕微鏡システムおよびそれを用いた検査方法
US7173259B2 (en) * 2004-06-09 2007-02-06 Taiwan Semiconductor Manufacturing Co., Ltd. Automatically aligning objective aperture for a scanning electron microscope
US7312448B2 (en) * 2005-04-06 2007-12-25 Carl Zeiss Nts Gmbh Method and apparatus for quantitative three-dimensional reconstruction in scanning electron microscopy
TWI275118B (en) * 2005-12-09 2007-03-01 Li Bing Huan Sample box of electron microscope for observing a general sample/live cell
US8577171B1 (en) * 2006-07-31 2013-11-05 Gatan, Inc. Method for normalizing multi-gain images
US9008378B2 (en) * 2006-12-20 2015-04-14 The Board Of Trustees Of The Leland Stanford Junior University Arrangement and imaging of biological samples
US7936913B2 (en) * 2007-08-07 2011-05-03 Nextslide Imaging Llc Network image review in clinical hematology
WO2009046445A1 (en) * 2007-10-04 2009-04-09 Halcyon Molecular Sequencing nucleic acid polymers with electron microscopy
US7659510B2 (en) * 2008-03-28 2010-02-09 Chih-Yu Chao Cryo-charging specimen holder for electron microscope
US8793075B2 (en) * 2008-10-31 2014-07-29 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US9060926B2 (en) * 2008-10-31 2015-06-23 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US9072799B2 (en) * 2008-10-31 2015-07-07 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US8788211B2 (en) * 2008-10-31 2014-07-22 The Invention Science Fund I, Llc Method and system for comparing tissue ablation or abrasion data to data related to administration of a frozen particle composition
US9072688B2 (en) * 2008-10-31 2015-07-07 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US20100111835A1 (en) * 2008-10-31 2010-05-06 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Compositions and methods for therapeutic delivery with frozen particles
US8603496B2 (en) * 2008-10-31 2013-12-10 The Invention Science Fund I, Llc Compositions and methods for biological remodeling with frozen particle compositions
US8731841B2 (en) * 2008-10-31 2014-05-20 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US20100111831A1 (en) * 2008-10-31 2010-05-06 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Compositions and methods for surface abrasion with frozen particles
US8485861B2 (en) * 2008-10-31 2013-07-16 The Invention Science Fund I, Llc Systems, devices, and methods for making or administering frozen particles
US8725420B2 (en) 2008-10-31 2014-05-13 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US20100111845A1 (en) * 2008-10-31 2010-05-06 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Compositions and methods for therapeutic delivery with frozen particles
US8551505B2 (en) * 2008-10-31 2013-10-08 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US9060931B2 (en) * 2008-10-31 2015-06-23 The Invention Science Fund I, Llc Compositions and methods for delivery of frozen particle adhesives
US20100111836A1 (en) * 2008-10-31 2010-05-06 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Compositions and methods for therapeutic delivery with frozen particles
US8545855B2 (en) * 2008-10-31 2013-10-01 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US20100111857A1 (en) 2008-10-31 2010-05-06 Boyden Edward S Compositions and methods for surface abrasion with frozen particles
US8545857B2 (en) * 2008-10-31 2013-10-01 The Invention Science Fund I, Llc Compositions and methods for administering compartmentalized frozen particles
US8603495B2 (en) * 2008-10-31 2013-12-10 The Invention Science Fund I, Llc Compositions and methods for biological remodeling with frozen particle compositions
US9050070B2 (en) * 2008-10-31 2015-06-09 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US9050317B2 (en) * 2008-10-31 2015-06-09 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US8762067B2 (en) * 2008-10-31 2014-06-24 The Invention Science Fund I, Llc Methods and systems for ablation or abrasion with frozen particles and comparing tissue surface ablation or abrasion data to clinical outcome data
US9060934B2 (en) * 2008-10-31 2015-06-23 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US8798933B2 (en) * 2008-10-31 2014-08-05 The Invention Science Fund I, Llc Frozen compositions and methods for piercing a substrate
US8551506B2 (en) * 2008-10-31 2013-10-08 The Invention Science Fund I, Llc Compositions and methods for administering compartmentalized frozen particles
US8731840B2 (en) * 2008-10-31 2014-05-20 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US8721583B2 (en) * 2008-10-31 2014-05-13 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US20100111834A1 (en) * 2008-10-31 2010-05-06 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Compositions and methods for therapeutic delivery with frozen particles
US8545856B2 (en) * 2008-10-31 2013-10-01 The Invention Science Fund I, Llc Compositions and methods for delivery of frozen particle adhesives
US8409376B2 (en) 2008-10-31 2013-04-02 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
WO2013138208A1 (en) * 2012-03-16 2013-09-19 Dennis Robert T Research on, identification of and screening for biological materials using low energy electron microscopy
JP5836221B2 (ja) * 2012-08-03 2015-12-24 株式会社日立ハイテクノロジーズ 荷電粒子線装置
TWI506262B (zh) * 2014-09-01 2015-11-01 Powerchip Technology Corp 穿透式電子顯微鏡試片的製備方法
CN108375599A (zh) * 2018-02-02 2018-08-07 华南农业大学 一种微小型昆虫复眼的透射电镜样品制片方法
CN109207552A (zh) * 2018-09-21 2019-01-15 云南省农业科学院生物技术与种质资源研究所 一种小西葫芦黄花叶病毒粒体的原位分离固定电子显微镜诊断方法
CN109136323A (zh) * 2018-09-21 2019-01-04 云南省农业科学院生物技术与种质资源研究所 一种马铃薯y病毒粒体的原位分离固定电子显微镜诊断方法
US11914131B1 (en) * 2020-08-16 2024-02-27 Gregory Dimitrenko Optical testing system for detecting infectious disease, testing device, specimen collector and related methods
CN112381817B (zh) * 2020-11-30 2021-06-25 中国科学院自动化研究所 扫描电镜透射模式与透射电镜联用的病毒快速检测系统
CA3224319A1 (en) 2022-06-29 2023-12-29 Tomas Bejdak A multi-mode low-voltage electron microscope

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Cited By (14)

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JP2008032531A (ja) * 2006-07-28 2008-02-14 Kao Corp 細菌の共凝集能の評価方法
US10557835B2 (en) 2010-10-29 2020-02-11 Thermo Fisher Scientific Oy Automated system for sample preparation and analysis
JP2013541718A (ja) * 2010-10-29 2013-11-14 サーモ・フィッシャー・サイエンティフィック・オイ サンプルの準備および分析のための自動化されたシステム
US10088460B2 (en) 2010-10-29 2018-10-02 Thermo Fisher Scientific Oy Automated system for sample preparation and analysis
US10739321B2 (en) 2010-10-29 2020-08-11 Thermo Fisher Scientific Oy Automated system for sample preparation and analysis
JP2014157772A (ja) * 2013-02-18 2014-08-28 Horon:Kk 走査型電子顕微鏡および検査装置
CN111065950A (zh) * 2017-09-15 2020-04-24 敏捷焦点设计有限责任公司 用于宽场、共焦和多光子显微镜的动态聚焦和变焦系统
CN111065950B (zh) * 2017-09-15 2022-05-03 敏捷焦点设计有限责任公司 用于宽场、共焦和多光子显微镜的动态聚焦和变焦系统
WO2019225507A1 (ja) * 2018-05-24 2019-11-28 株式会社ニコン 生物組織画像処理装置及び方法
JP2019203803A (ja) * 2018-05-24 2019-11-28 日本電子株式会社 生物組織画像処理システム及び機械学習方法
WO2019225506A1 (ja) * 2018-05-24 2019-11-28 株式会社ニコン 生物組織画像処理システム及び機械学習方法
JP2019204695A (ja) * 2018-05-24 2019-11-28 日本電子株式会社 生物組織画像処理装置及び方法
JP7181003B2 (ja) 2018-05-24 2022-11-30 日本電子株式会社 生物組織画像処理装置及び方法
JP7181002B2 (ja) 2018-05-24 2022-11-30 日本電子株式会社 生物組織画像処理システム及び機械学習方法

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EP1437758A2 (en) 2004-07-14
US20040135083A1 (en) 2004-07-15
US6875984B2 (en) 2005-04-05

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