JP2004196750A - Stress reliever - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a stress reliever containing an extract of black cohosh (Cimicifuga racemosa) as an active ingredient and to provide a food, a functional food, a beverage and a medicine each containing the stress reliever. <P>SOLUTION: The stress reliever comprises the extract of black cohosh as the active ingredient. In the stress reliever, black cohosh itself or its extract can be used. The black cohosh extract is obtained by extracting the black cohosh with water, ethanol or water-containing ethanol or other organic solvent and concentrating the extracted solution or purifying the resultant extract by using an ultrafiltration membrane. Although the leaf, stem, flower, rhizome, etc., of black cohosh can be used for extraction, the rhizome can preferably be used. There is further provided a composition containing the black cohosh extract and having stress-relieving action and the composition has preferably a form of food, dietary supplement, functional food or medicine. <P>COPYRIGHT: (C)2004,JPO&NCIPI

Description

[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a stress relieving agent containing black cohosh or an extract thereof as an active ingredient.
[0002]
[Prior art]
Modern times are a time of stress overload, and more or less live today, under the influence of stress. In recent years, the effects of such stress on the human body have been investigated in detail, and it is becoming clear that unexpectedly large effects are exerted. It is said that many criminal cases that have a shocking impact on society in recent years are caused by such stress. Reducing the cause of stress is a fundamental solution, but at present it is difficult, and there is a demand for a food material that reduces the effects of stress.
[0003]
BACKGROUND ART A plant extract and a Chinese herbal extract that are expected to recover from the effects of stress and improve depression due to stress are known. For example, Leguminosae magnolia, Citrus spruce, Lily family aloe, Oleum spp. An agent for promoting recovery from the adverse effects of stress consisting of the essence of the herbal crude drug of the family Kyoukatsu (see, for example, Patent Literature 1), an anti-depressant food containing Lufu hemp extract, a dietary supplement, and a pharmaceutical (For example, see Patent Document 2).
[0004]
Black cohosh is a plant belonging to the family Ranunculaceae under the scientific name of Cimicifuga racemosa. It is known that its main component, Cimicifugin, suppresses a rapid decrease in estrogen and relieves the symptoms of menopause. For the use of black cohosh extract, as an estrogen-type time-selective drug for the treatment and / or prevention of cardiovascular diseases, in particular atherosclerosis, osteoporosis and menopause, for example to prevent or reduce hot flashes. It is disclosed (Patent Document 3). However, no stress relief effect is mentioned.
[0005]
[Patent Document 1] Japanese Patent Application Laid-Open No. 2001-192338 [Patent Document 2] Japanese Patent Application Laid-Open No. 2002-201139 [Patent Document 3] Japanese Patent Application Laid-Open No. 2002-506727
[Problems to be solved by the invention]
An object of the present invention is to provide a stress relieving agent which is naturally derived and safe even when taken for a long time.
[0007]
[Means for Solving the Problems]
Means for Solving the Problems As a result of intensive studies, the present inventors have found that a black cohosh extract exhibits a stress relieving effect, and have completed the present invention.
[0008]
Accordingly, an object of the present invention is to provide a stress relieving agent containing a black cohosh extract as an active ingredient. Further, the present invention provides a food, a functional food, a beverage, and a medicine containing the stress relieving agent.
[0009]
BEST MODE FOR CARRYING OUT THE INVENTION
As the stress relieving agent in the present invention, black cohosh itself or an extract thereof can be used. The black cohosh extract is an extract obtained by extracting and concentrating black cohosh with water or ethanol or aqueous ethanol or an organic solvent, and an extract obtained by purifying the extract obtained here using an ultrafiltration membrane. is there. Black cohosh leaves, stems, flowers, rhizomes and the like can be used for extraction, but rhizomes are preferred. The hydrated ethanol has an ethanol concentration of 10 to 95%, preferably 30 to 70%, and more preferably 50%.
[0010]
The present invention further provides a composition having a stress-relieving action comprising a black cohosh extract, which composition is preferably in the form of a food, dietary supplement, functional food or pharmaceutical product. The method of administration when the composition of the present invention is a pharmaceutical composition is not particularly limited, but a dosage form capable of oral administration is preferred. The pharmaceutical composition of the present invention can be in various dosage forms. For example, for oral administration, tablets, capsules, powders, granules, pills, solutions, emulsions, troches, suspensions, solutions, alcoholic beverages, syrups, extracts, elixirs But not limited to these. In addition, various pharmaceutically acceptable carriers can be added to the preparation. For example, excipients, binders, disintegrants, lubricants, flavoring agents, coloring agents, sweeteners, flavoring agents, solubilizing agents, suspending agents, emulsifiers, coating agents, vitamin C, antioxidants But may be, but not limited to.
[0011]
The dose of the pharmaceutical composition of the present invention is generally 1 mg to 2000 mg, preferably 1 mg to 1000 mg, as a daily dose for an adult in terms of black cohosh extract. Of course, it can also be varied individually depending on the age, weight, condition, administration route, administration period, treatment course, etc. of the human being administered. The daily dose can be administered in several divided doses. It can also be administered in combination with other stress relievers and treatments.
[0012]
The compositions of the present invention can also be in the form of a food or dietary supplement. For example, by blending the black cohosh extract with the raw material, it can be made into the form of noodles, bread, candy, jelly, cookies, soup, and health drink. Such foods, dietary supplements, functional foods other than black cohosh extract, iron, inorganic components such as calcium, various vitamins, oligosaccharides, dietary fiber such as chitosan, protein such as soy extract, Lipids such as lecithin and sugars such as sucrose and lactose can be added.
[0013]
In addition, the black cohosh extract of the present invention contains Rafu hemp extract, Korean ginseng extract, Ginkgo biloba extract, Tubulus extract, Eleuthero extract, Forage extract, Guarana extract, Maca extract, Dandelion extract, Artichoke extract , Gentian extract, sisandra extract, western squash extract, soybean extract, licorice extract, toki extract, western carrot extract, pomegranate extract, yam extract, oak radishes extract, kidachi aloe extract, The combination of the extract of ginseng ginseng, the extract of radishes, the extract of mistletoe, and the like can provide compositions such as foods, dietary supplements, functional foods, and pharmaceuticals that have the effect of alleviating the symptoms of mental stress.
[0014]
【The invention's effect】
The black cohosh extract of the present invention exhibits a stress relieving effect and is useful as a component of a stress relieving agent. In addition, the black cohosh extract can be used in compositions such as foods for reducing stress, dietary supplements, functional foods, and pharmaceuticals.
[0015]
【Example】
Example 1 Purification of black cohosh extract 5.2 kg of rhizome of black cohosh was extracted with 10 liters of 50% aqueous ethanol, concentrated, and then dried to obtain 445 g of an extract.
[0016]
Example 2 Examination of Stress Relief Action The study was performed using 6-week-old BALB / c male mice. After pre-breeding for 1 week, at the age of 7 weeks, 1.0 g / kg, 0.5 g / kg, or 0.2 g / kg of the black cohosh extract obtained in Example 1 was orally administered. Thirty minutes after the administration, the mice were loaded with a stress for confining them in a 30 ml syringe for injection for one hour, and blood was immediately collected to measure the corticosterone concentration, the GOT concentration, the LDH concentration, and the IL-6 concentration in the plasma.
As shown in Table 1, the test group significantly suppressed the increase of each parameter in a dose-dependent manner as compared with the control group.
[0017]
Table 1 Dose-dependent corticosterone (ng / ml) on stress relief effect of black cohosh GOT (karmen) LDH (IU / l) IL-6 (pg / ml)
Control group 559.5 ± 49.0 46.8 ± 3.4 921.7 ± 44.7 7.1 ± 0.6
1.0g / kg group 354.6 ± 20.6 ** 19.5 ± 1.0 ** 339.4 ± 24.2 ** 4.8 ± 0.3 **
0.5g / kg group 335.2 ± 28.7 ** 26.6 ± 1.5 ** 558.2 ± 63.2 ** 4.6 ± 0.3 **
0.2g / kg group 376.9 ± 40.4 ** 28.7 ± 3.9 ** 462.4 ± 68.8 ** 5.2 ± 0.4 *
(N = 5, mean ± standard deviation, ** p <0.01, * p <0.05)
[0018]
Example 3 Examination of effect with female mice The study was conducted using 6-week-old BALB / c female mice. After pre-breeding for one week, at the age of seven weeks, 1.0 g / kg of the black cohosh extract obtained in Example 1 was orally administered. Thirty minutes after the administration, a stress for confining the mouse in a 30 ml syringe for injection was applied for 1 hour, and blood was immediately collected to measure corticosterone concentration, GOT concentration, and LDH concentration in plasma.
As shown in Table 2, similarly to the male group, the test group significantly suppressed the increase of each parameter as compared with the control group.
[0019]
Table 2 Stress relief effect of black cohosh on female sex corticosterone (ng / ml) GOT (karmen) LDH (IU / l)
Control group 719.1 ± 53.9 44.1 ± 5.0 805.2 ± 121.1
1.0g / kg group 480.5 ± 19.5 ** 22.0 ± 2.1 ** 303.6 ± 27.2 **
(N = 5, mean ± standard deviation, ** p <0.01)
[0020]
Example 4 Examination of Active Ingredient Extraction Method The black cohosh extract (30 g) obtained in Example 1 was fractionated into a water-soluble fraction (1 g) and a water-insoluble fraction (29 g), and a confirmation test was carried out in the same manner as described above. Was. As a result, a strong activity was confirmed in the water-insoluble fraction. (Table 3)
[0021]
Table 3 Examination of active ingredient extraction method of black cohosh Corticosterone (ng / ml) GOT (karmen) LDH (IU / l)
Control group 600.8 ± 61.1 43.5 ± 5.6 1314.9 ± 198.3
Water-soluble fraction group 525.8 ± 99.8 26.3 ± 2.8 * 706.7 ± 116.4 *
Water-insoluble fraction group 462.7 ± 39.1 19.4 ± 0.4 ** 499.8 ± 16.6 **
(N = 5, mean ± standard deviation, ** p <0.01, * p <0.05)
In the following examples, a group of water-insoluble fractions of the black cohosh extract obtained in this example was used.
[0022]
Example 5 (troche)
Gum arabic 6.0
Magnesium stearate 3.0
Glucose 73.0
Lactose 17.6
Potassium phosphate 0.2
Potassium phosphate 0.1
Fragrance 0.095
Water-insoluble fraction group obtained in Example 4 0.005
Total 100.0
A lozenge was prepared according to a conventional method using each of the above parts by weight.
[0023]
Example 6 (candy)
Sucrose 20.0
Candy syrup (75% solids) 70.0
Water 9.5
Coloring agent 0.45
Fragrance 0.045
Water-insoluble fraction group obtained in Example 4 0.005
Total 100.0
A candy was prepared according to a conventional method using the above components in each part by weight.
[0024]
Example 7 (chewing gum)
Gum base 20.0
Calcium carbonate 2.0
Lactose 77.0
Stevioside 0.095
Water-insoluble fraction group obtained in Example 4 0.005
Fragrance 0.9
Total 100.0
Chewing gum was prepared according to a conventional method using the above components in each part by weight.
[0025]
Example 8 (juice)
Concentrated orange juice 15.0
Fructose 5.0
Citric acid 0.2
Fragrance 0.1
Pigment 0.15
Sodium ascorbate 0.048
Water-insoluble fraction group obtained in Example 4 0.002
79.5 water
Total 100.0
A juice was prepared using the above-mentioned respective parts by weight and in accordance with a conventional method.
[0026]
Example 9 (Cookie)
Soft flour 32.0
Whole egg 16.0
Butter 16.0
Sugar 25.0
Water 10.8
Baking powder 0.198
Water-insoluble fraction group obtained in Example 4 0.002
Total 100.0
A cookie was prepared according to a conventional method using the above components in each part by weight.
[0027]
Example 10 (caramel)
Granulated sugar 31.0
Candy syrup (75% solids) 20.0
Powdered milk 40.0
Hardened oil 5.0
Salt 0.6
Fragrance 0.025
Water-insoluble fraction group obtained in Example 4 0.005
Water 3.37
Total 100.0
Using the above-mentioned respective parts by weight of each component, caramel was prepared according to a conventional method.
[0028]
Example 11 (tablets, capsules)
Water-insoluble fraction group obtained in Example 4 10.0
Lactose 75.0
Magnesium stearate 15.0
Total 100.0
The above parts by weight were uniformly mixed to obtain tablets and capsules according to a conventional method.
[0029]
Example 12 (powder, granule)
The water-insoluble fraction group obtained in Example 4 20.0
Starch 30.0
Lactose 50.0
Total 100.0
The above parts by weight were uniformly mixed to give powders and granules in a conventional manner.
[0030]
Example 13 (injection)
The water-insoluble fraction group obtained in Example 4 1.0
Surfactant 9.0
Physiological saline 90.0
Total 100.0
Each of the above parts by weight was heated, mixed and sterilized to give an injection.

Claims (4)

A stress relieving agent containing black cohosh as an active ingredient. A stress relieving agent containing a black cohosh extract as an active ingredient. A composition having a stress relieving effect, comprising black cohosh or an extract thereof. 4. The composition according to claim 3, wherein the composition is a food, a dietary supplement, a functional food or a pharmaceutical.