JP2004113061A - Method for rearing small organism such as mite, selection utensil, rearing vessel, rearing vessel collector and laying vessel - Google Patents

Method for rearing small organism such as mite, selection utensil, rearing vessel, rearing vessel collector and laying vessel Download PDF

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JP2004113061A
JP2004113061A JP2002278978A JP2002278978A JP2004113061A JP 2004113061 A JP2004113061 A JP 2004113061A JP 2002278978 A JP2002278978 A JP 2002278978A JP 2002278978 A JP2002278978 A JP 2002278978A JP 2004113061 A JP2004113061 A JP 2004113061A
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mites
breeding
container
rearing
cylindrical case
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JP3680135B2 (en
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Yukio Chikayama
近山 之雄
Yukio Yagi
八木 行雄
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National Agriculture and Bio Oriented Research Organization NARO
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National Agriculture and Bio Oriented Research Organization NARO
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for rearing small organisms such as mites, by which the small organisms can efficiently, easily, safely, rapidly and successively be controlled and reared, to provide a rearing vessel, and to provide a rearing utensil. <P>SOLUTION: This method for rearing the small organisms such as mites is characterized by putting the small the small organisms such as the hatched mites in a selection vessel 1 to separate the small organisms from undesired materials, collecting the separated mites or the like, rearing the collected mites or the like in a rearing vessel 10 for a prescribed period, and then making the grown mites or the like to lay in a laying bed 30 in which laying spaces 35 are disposed in parallel. The separation of the mites is carried out by using the selection vessel 1 having the space of an isolation portion 5 in the center and also having a sponge 6 used as a wall containing water in the outer peripheral portion and utilizing the water-avoiding nature of the mites to separate the mites from the undesired materials. The rearing vessel 10 comprises a cylindrical case 11, an upper lid 15 and a bottom stopper 14 for closing the upper and lower openings of the case, a water supply tank 13 on the bottom stopper, and a mite bed 12 on the water supply tank. <P>COPYRIGHT: (C)2004,JPO

Description

【0001】
【発明の属する技術分野】
本発明は、ダニ等の微小生物を継代管理する飼育方法と飼育容器等に係り、特に、ダニ等を選別する容器状の選別器具、ダニ等を容易に容器外へ出すことができる飼育容器、飼育容器にダニ等を収集する収集器具、ダニ等の産卵用の容器と、これらの選別器具、飼育容器等を使用する飼育方法に関する。
【0002】
【従来の技術】
従来、この種のダニ等の微小生物を継代管理して飼育する器具としては、大試験管の中に含水させた綿を入れ、その上にスポンジを敷き、その上にダニを入れた後、綿栓を装着することでダニの飼育容器もしくは産卵容器としていた。また、飼育容器である容器の交換、及び耳袋法によるダニ付けには、ダニを容器から少しずつバット又は耳袋に取出していた。ここで、耳袋法によるダニ付けとは、ダニを布袋等に入れ、家畜等の耳につけて飽血させることである。
【0003】
【発明が解決しようとする課題】
ところで、前記のような飼育器具によって継代管理して飼育する方法では、ダニは乾燥とカビに弱いので、乾燥防止と飼育容器内のダニの脱皮による抜け殻や排泄物等によるカビの発生防止のため、適宜、新しい飼育容器に交換しなければならないが、飼育容器内に付着するダニは取出しにくく作業効率が低いという問題がある。
【0004】
また、ダニを新しい飼育容器へ吸引回収する際、一時的に容器の外に出す必要があるものの、ダニは雲散逃走するために逃がしてしまう危険が高いという問題がある。ダニの吸引回収に際しては吸引器を使用するが、試験管等の容器に連結する吸引ジョイント底部にダニが群がり、吸引ジョイントと栓の交換時にダニを逃がす虞が高いという問題がある。さらに、ダニの系統的な継代を行うには、飽血成ダニを飼育容器1本につき1匹ずつ入れて管理しなければならず、飼育スペースがかさむという問題もある。
【0005】
本発明は、このような問題に鑑みてなされたものであって、その目的とするところは、ダニ等の微小生物の系統的な継代管理作業を容易に、安全に、しかも迅速に行え、ダニ等を効率よく飼育できる飼育方法を提供することにある。また、前記の飼育方法に使用し、ダニ等の微小生物を選別し、収集する器具や、効率よく飼育できる飼育容器、産卵容器を提供することを目的とする。
【0006】
【課題を解決するための手段】
前記目的を達成すべく、本発明に係るダニ等の微小生物の飼育方法は、孵化したダニ等の微小生物を選別器具に入れて死骸等の不要物から自発的移動によって分離させて収集し、収集された微小生物を飼育容器内に所定期間入れて飼育し、成長した微小生物を複数の産卵スペースを並設した産卵容器に入れて産卵させることを特徴とする。ダニ等の選別は、容器状の選別器具で外周部に水分を含んだ壁部を有する選別器具内の中央部にダニ等を入れて、死骸等の不要物から分離する。ダニ等の収集は、吸引器等を用いて飼育器に直接吸引して収集しても、スポイト等を用いて収集して飼育器に入れてもよい。
【0007】
この飼育方法によれば、ダニは雲散すると共に水分を避ける性質があるため、選別容器内で死骸、排泄物、脱皮した抜け殻等の不要物から容易に選別することができる。また、飼育容器内に吸引されて容易に入れることができ、汚れ防止のための飼育容器の交換も容易に行えるため清潔な環境内で飼育され、弱ったり死んだりすることを防止できる。そして、所定の期間飼育されて成長したダニ等は、飼育容器から出されて産卵容器に入れられ、少ないスペースで集約的に産卵の管理を行うことができる。このように、ダニの選別、飼育容器の交換や、産卵容器への移動が容易にできるため作業効率がよく、飼育容器内を清潔に保つことができ、ダニ等の継代管理を安全に迅速に行うことができる。
【0008】
また、本発明に係る選別器具は、前記の飼育方法で使用するもので、ダニ等の微小生物を死骸等の不要物から分離させる容器状の選別器具であり、中央部に空間を有し、外周部に微小生物を回避させる物質を含んだ壁部を有することを特徴とする。微小生物を回避させる物質としては、ダニ等の微小生物が好まない水分やアルコール等の物質が用いられる。この選別器具は白色で扁平の円形状容器が好ましく、外周側の壁と内周側の壁との間にスポンジ等の吸水性素材を入れて水分を含んだ壁部とすると好適である。
【0009】
このように構成された選別器具によれば、飼育中の多数のダニを中央部の空間に入れると、ダニは四方に向けて雲散移動し、外周の水分を含んだ壁部に接近すると水分を避けて後退する。このため、中央部の死骸、排泄物、脱皮した抜け殻等からダニを容易に選別して分離することができ、逃走も防止できる。ダニは気温が高いと活発に動き回るが、気温が低いと活動を抑制または休止するため、本発明の選別器具を冷房下で使用することによって外周の壁部の水分等が冷やされ、ダニの動きを抑制することができるため、さらにダニを効率良く不要物から分離して隔離することができる。また、壁部にアルコールを適度に含ませると、アルコールが気化して外周の壁部の熱を奪い、壁部の温度を下げる効果によりダニを効果的に隔離することができる。なお、アルコールはダニにとって有害であるため、使用にあたってはできるだけ低濃度で使用することが好ましい。
【0010】
本発明に係る飼育容器は、前記の飼育方法で使用するもので、ダニ等の微小生物を飼育する飼育容器であって、円筒状ケースと、この円筒状ケースの下端の開口部を閉塞する底栓と、この底栓の上部に位置する給水手段と、この給水手段の上部に位置して該給水手段により水分を供給される飼育床と、円筒状ケースの上部開口を閉塞する上蓋とを備えることを特徴としている。給水手段としては、水を貯える給水タンクや水を含ませた綿等を使用することができる。上蓋は通気性を有し、ダニ等の逃走を防止するスポンジ等の蓋が好適である。また、この飼育容器は、底栓及び給水手段を外して円筒状ケース内に下方から挿入され、飼育床を上方に押上げて円筒状ケース外に移動できるピストンをさらに備えることが好ましい。
【0011】
この飼育容器によれば、容器内にダニが入ったままの状態で容易に給水することができ、容器内を適度な湿度に保つことができるため、ダニ等の飼育管理を容易に行うことができる。また、飼育中のダニは円筒状ケースの内壁に付着しているがピストンを使用して飼育床を押上げて一度に容器外へ出すことができ、円筒状ケースの洗浄も容易に行える。
【0012】
本発明に係る収集器具は、前記の飼育容器の円筒状ケースに装着され微小生物を収集する収集器具であって、円筒状ケース内に挿入可能の壁面を有し下方に開口するホルダと、このホルダの下方の開口を閉塞して内部空間を形成する下栓と、ホルダの上部に連結され前記円筒状ケースの開口を閉塞する上栓と、ホルダの壁面に下方から形成され内部空間に至るように形成された切込みと、上栓及び下栓を貫通するパイプ及び上栓を貫通するパイプとを備えることを特徴とする。
【0013】
この収集器具の構成によれば、前記のダニ等を選別する選別容器で不要物から自発的移動によって分離されたダニを飼育容器に入れるとき、上栓を貫通するパイプに吸引器を連結し、下栓を貫通するパイプに連結したチューブ等をダニ等に接近させると、ダニ等はチューブを経由して下栓まで貫通するパイプを通して飼育容器の飼育床の上部空間まで入れられる、そして、収集器具を持ち上げ、ホルダの切込みにピンセット等の工具を挿入して下栓を円筒状ケースに残したまま収集器具を円筒状ケースから取外すことができるため、ダニが飼育容器外に逃走することを防止できる。
【0014】
さらに、本発明に係る産卵容器は、前記の飼育方法で使用するもので、ダニ等の微小生物が産卵する産卵容器であって、水平方向に並設した上方開口の複数の区画と、この複数の区画の下部に位置する給水区画と、この給水区画と複数の区画のそれぞれとを連通する貫通孔と、複数の区画の上方開口を閉塞する蓋体とを備えることを特徴としている。複数の区画は1列に並べたものや、縦横に並べたもの等、適宜選択できる。複数の区画内に上方開口のメッシュ材として、ガーゼ等を押込んで挿入すると好ましい。
【0015】
このように構成された本発明の産卵容器は、成長して十分に血を吸った飽血成ダニを水平方向に並設した上方開口の複数の区画である産卵スペースに入れ、全ての産卵スペースを一目で確認できるため集約的に、少ないスペースで管理することができる。また、下部に位置する給水区画から貫通孔を通して気化した水分が適度に産卵スペースに供給されるため、産卵の環境が整ってダニの個別継代を容易に管理することができる。産卵スペースにガーゼ等を挿入すると、ダニが起き上がりやすく、ダニの横転を防止できるため、ダニ等が弱ることを防止でき迅速に産卵させることができる。
【0016】
【発明の実施の形態】
以下、本発明に係るダニ等の微小生物の飼育方法に使用する飼育容器及び選別器具等として、先ず、ダニを選別する容器状の器具として選別容器の一実施形態を図面に基づき詳細に説明する。図1は、本実施形態に係る選別容器の斜視図、図2は、図1のA−A線断面図である。
【0017】
図1,2において、選別容器1はフタトゲチマダニ等の微小生物を選別回収する際、ダニの水を避ける性質を利用することで一時的にダニを容器内に隔離するものである。選別容器1はプラスチックや金属製の底壁2と外周壁3から構成される容器状をしており、円周状の外周壁3から内側に所定の距離をおいて円周状の内周壁4が形成され、外周壁3より内周壁4の高さが小さく設定されている。そして、内周壁4の内側の空間はダニの隔離部5となっている。選別容器1は、例えば白色の合成樹脂から円形の扁平容器状に形成すると、ダニを見つけやすく好都合である。
【0018】
ダニの隔離部5の外周側は、外周壁3と内周壁4に挟まれたリング状の空間となっており、この空間に吸水性素材としてリング状のスポンジ6がはめ込まれている。そして、スポンジ6には使用時には十分に水が含水される。このように、選別容器1は中央部に隔離部5の空間を有し、外周部に水分を含んだリング状のスポンジ6が位置して壁部となっている。なお、スポンジ6には、ダニを回避させる性質を有するアルコールを含ませるようにしてもよい。
【0019】
このスポンジ6の高さは、外周壁3より低く、また内周壁4より高く形成されている。内周壁4は、ダニ同士がそれぞれ背中を上り架け橋を作って逃走するのを防止するため、ある程度の高さを持たせてある。なお、吸水性素材としては、スポンジに限らず綿やグラスウール等、水を十分に含むものであれば、どのようなものを使用してもよい。また、スポンジ6は図2に示すように内周壁4の上端面を覆う突出部6aを有することが好ましい。
【0020】
前記の如く構成された本実施形態のダニ等を選別する選別容器1の動作について以下に説明する。飼育中のダニを選別容器1の中央部の隔離部5に入れると、中央部には生存しているダニと共にダニの死骸、ダニの排泄物、脱皮した抜け殻等が集中して置かれる。そして、時間が経過すると、ダニは四方に向かって雲散逃走し、中央部には不要物のみが残る。内周壁4を上ったダニは吸水したスポンジ6に触れると後退して底面に戻るため、生きているダニは底面の周辺部に集まり、自発的移動によって分離される。このため、この部分を吸引することにより実験用のダニを分離させて収集することができ、不要物から選別することができる。
【0021】
つぎに、ダニ等の飼育容器として、円柱状の飼育容器の例を図3,4に基づいて説明する。飼育容器10は透明又は半透明の円筒状ケース11を備え、円筒状ケース11の中央部にスポンジ等で形成される飼育床として円柱状のダニ床12が嵌合され、ダニ床12の底面に接するように給水手段として給水タンク13が挿入され、円筒状ケース11の下端には給水タンク13を支持する底栓14が嵌合固定され、円筒状ケース11の上端にはスポンジ等で形成された通気性を有する上蓋15が嵌合され、上蓋15とダニ床12との間のスペースが飼育空間11aとなっている。ダニ床12は飼育空間11aに水分を供給しダニの乾燥を防止すると共に、ダニ及びダニの死骸や抜け殻を受ける役目を有している。
【0022】
給水タンク13は図3(c)に示すように、円筒状ケース11に緩く挿入される外径を有する給水容器13aと、この給水容器の上部開口に嵌合される吸水芯13bとから構成される。吸水芯13bは給水容器内の水を吸い上げてスポンジのダニ床12に水分を供給するものであり、上部は給水容器13aの内周に嵌合する大径部であり、この大径部の中央から下方に垂下し水を吸い上げる小径部13cを備えており、全体がスポンジ等の吸水性素材で一体的に形成されている。
【0023】
底栓14は円錐台状のゴム栓から形成され、円筒状ケース11の下方開口に固く嵌合して給水タンク13が落下するのを防止している。上蓋15はスポンジや綿等から形成され、ダニ床12上で飼育中のダニに空気を透過して与えるものであると共に、ダニが円筒状ケース11の内面を登ってきたときに、円筒状ケース内から逃走するのを防止している。
【0024】
飼育容器10は、図3(b)に示す円筒状ケース11内に挿入されるピストン16をさらに備える。ピストン16は上部が円筒状ケース11の内面に緩く嵌合する押上げ部16aであり、下端は指で押す操作部16bとなっており、中間は連結軸部16cとなっている。このピストン16は底栓14及び給水タンク13を外して円筒状ケース11内に下方から挿入され、図4に示すようにダニ床12を押上げて円筒状ケース11外に移動し、ダニを容器外に出すものである。飼育容器10の底栓14及び給水タンク13を外してピストン16を挿入することにより、飼育容器10は注射型となる。
【0025】
この円柱状の飼育容器10は、ダニ床12でダニを飼育しながら底栓14を外して給水タンク13を取出して給水できるため、ダニの飼育管理が容易に行える。また構成が簡単で、全てを外すと円筒状ケース11のみとなり、洗浄が容易で清潔に保つことができる。そして、飼育中のダニを移動するときは、底栓14を外して給水タンク13を抜き、ピストン16を下方から挿入してダニ床12を押上げて上蓋15と共に押出すことにより容易に移動できる。
【0026】
ダニ床12の側面あるいは全面をポリエチレン製等の滑りやすい素材のネット(図示せず)で巻くあるいは包むことにより、ダニ床をスライドさせる際の摩擦抵抗を少なくしてスムーズに押出すことができる。すなわち、円筒状ケース11がガラス製であると、洗浄によって内壁面に見えない細かい傷が付き、ダニ床12をピストン16で押上げようとすると、内壁面とダニ床間の摩擦が生じてダニ床の捻れ等が起こり、スムーズにダニ床12を押出すことができなくなるのを防止することができる。
【0027】
本発明に係る収集器具の実施形態として、ダニを図3に示す飼育容器10に入れるため円筒状ケース11に装着される吸引ジョイント20の例を図5,6に基づいて説明する。この吸引ジョイント20は、前記した注射型の飼育容器10にダニ等を効率よく収集して入れるためのジョイント用の器具であり、飼育容器10の円筒状ケース11に挿入できる外径の円筒壁面を有し下方に開口する容器状のホルダ21と、ホルダ21の下方開口を塞ぐスポンジ等から形成され仮栓として機能する下栓22と、ホルダ21の上部に止めねじ21aで連結されたゴム栓等の上栓23とを備えている。止めねじ21aはホルダ21の中心の1本でもよく、円周上に2本あるいは複数本設ける構成でもよい。
【0028】
上栓23は反転した円錐台状をしており、上端の外径は飼育容器10の円筒状ケース11の内径より大きく形成されている。下栓22は円錐台状をしており、下端の直径は飼育容器10の円筒状ケース11の内径より大きく形成されている。なお、下栓は円錐台状に限らず、円柱状に形成してもよい。この場合、下栓の外径は円筒状ケースの内径より大きく形成し、ホルダの内周に嵌合させると共に、円筒状ケースの内壁と密着させて隙間を塞ぐようにする。
【0029】
ホルダ21は透明プラスチックや金属等から形成され、下方の開口は下栓22により閉塞され、内部空間24が形成されている。そして、ホルダ21の外周面には下方から軸方向に2本の切込み25が形成され、切込み25は内部空間24に至るように形成されている。このため下栓22を避けて内部空間24にピンセット等の工具を挿入することができる。上栓23を貫通する長い吸引パイプ26は下栓22も貫通している。上栓23を貫通するもう1本の短い吸引パイプ27は内部空間24に開口している。吸引ジョイント20は、ダニ等を吸引収集して飼育容器10に入れる吸引器(図示せず)の吸引ジョイントとして機能する。
【0030】
なお、吸引ジョイントは、図5(c)に示す吸引ジョイント20Aのように、ホルダ21Aを円筒から形成し、上栓23Aをホルダ21Aの上方開口に嵌合して連結してもよい。この例では、ホルダ21Aに形成された切込み25Aは1本であるが、2本でもよいことは勿論である。
【0031】
吸引ジョイント20を使用するときは、図3に示す飼育容器10の上蓋15を取外し、図6(a)に示すように、円筒状ケース11の上部開口から吸引ジョイント20の下栓22及びホルダ21を挿入して上栓23を嵌め込む。そして、上栓23の吸引パイプ27に図示していない吸引器を連結パイプでつなぎ、吸引パイプ26につないだチューブでダニを吸入すると、ダニは矢印Y1のようにダニ床12と下栓22との間の飼育空間11aに入れられ、排気は矢印Y2のように出すことができる。飼育容器にダニを吸引するときは、給水タンク13を外した状態で行い、ダニを吸引回収後に給水タンク13を挿入し、円筒状ケース11に底栓14を嵌合して固定する。
【0032】
ダニを吸引回収し、飼育空間11aに入れたあと、図6(b)に示すように、下栓22を円筒状ケース11の上端と一致する位置まで吸引ジョイント20を引上げる。そして、切込み25を通して内部空間24にピンセットなどの工具28を差し入れ、下栓22を押えて移動を防止しながら吸引ジョイント20のホルダ21から上の部分を引抜く。その結果、図6(c)に示すように、円筒状ケース11に下栓22が残るため、ダニは下栓22によりブロックされて上方への移動が阻止され、ダニの逃走を防止できる。なお、下栓22がスポンジで形成されているため、下栓22を貫通する吸引パイプ26が引抜かれた孔26aは復元力で自然に閉塞するので、ダニの逃走を防止できる。
【0033】
本発明の産卵容器の実施形態として、ダニが産卵する産卵ベッドの例を図7,8に基づいて説明する。産卵ベッド30は、上方に開口する長方形状の下ケース31と、下ケース31の開口に着脱自在に嵌合し複数の区画を形成した中間ホルダ部32と、中間ホルダ部の上部を覆う上ケース33とから構成される。下ケース31は産卵用に飼育されているダニに水分を供給するための水を貯留するものであり、例えば長方形状のプラスチックケースで構成される。
【0034】
中間ホルダ部32は下ケース31の内側に嵌合する外周壁部32aと、外周壁部の内側底面を形成する底壁部32bと、外周壁部の外側に延出するフランジ部32cとを備え、フランジ部32cが下ケース31の上端に当接しているときに底壁部32bと下ケース31の底面との間に給水区画である空間Sが形成されるように設定されている。底壁部32bから上方に延出する円周壁部32dにより産卵スペース35として使用される複数の区画が形成され、図示の例では24個の円周壁部が形成されている。そして、例えば角部に位置する1つの区画は底壁部が貫通しており、下ケース31の空間Sへの給水部34となっている。各区画の底壁部32bには中心部に貫通孔36が形成され、下ケース31の空間Sと複数の産卵スペース35とがそれぞれ連通している。上ケース33の下面には、円周壁部32dの外周に嵌合する円周状の突部33aが形成され、ダニが上方に逃げるのと、隣の区画に移動するのを防止している。
【0035】
複数の産卵スペース35にはメッシュ材37を敷き詰めることで、ダニの起きあがりをサポートし、ダニの横転を防止するように構成した。メッシュ材37としては、例えばガーゼを指で押し込んで、上部が開口する容器状に形成しているが、これに限られるものでなく、合成樹脂でメッシュ状に形成した容器でもよい。産卵スペース35には下方の下ケース31に給水された水が気化して貫通孔36から上昇して適度な水分が保たれており、産卵に適した環境となっている。複数の産卵スペース35は水平方向に縦横に並設されているため、ダニの状況や産卵状況を一目で容易に把握することができる。
【0036】
本発明に係るダニ等の微小生物の飼育方法は、第1のステップとして、図1,2に示された選別容器1を使用してダニ等の微小生物を不要物から分離して選別する。すなわち、ダニは飼育中に弱って死んだり、脱皮によって抜け殻が散乱したり、排泄物にまみれて不潔になったりする。このような不潔な飼育環境から生きているダニを選別する。例えば試験管で飼育されているダニを選別容器1の中央部に入れると、ダニは時間と共に四方に向けて雲散し、早足のダニは内周壁4を上って含水されたスポンジ6に接近するが、ダニは水を避ける性質があるため、スポンジ6に接すると後退して選別容器1の底面の外周部に自発的移動によって分離され集中する。前記のように、壁部であるスポンジ6に低濃度のアルコールを含ませることによって、ダニを分離してもよい。
【0037】
第2のステップとして、選別容器1の外周部に分離されたダニを円柱状の飼育容器10に入れる。このとき、飼育容器10の上部開口に連結してダニを吸引して収集する収集器具として吸引ジョイント20を使用する。吸引ジョイント20の上栓23を飼育容器10の円筒状ケース11の上方開口に嵌め込み、短い吸引パイプ27に電動吸引ポンプ等の吸引器を連結し、長い吸引パイプ26に連結したチューブを選別容器1の隔離されたダニの部分に接近させる。吸引器を作動させるとダニはチューブを通して飼育容器10の飼育空間11a内に吸入され、排気は短いパイプから吸引器を通して排出される。なお、吸引パイプ27の途中にフィルタを設置して、吸引器にダニが吸い込まれるのを防止するようにしてもよい。
【0038】
ダニの飼育容器10への吸入がすんだあと吸引ジョイント20を飼育容器10から外すが、前記したように吸引ジョイント20を引上げて切込み25にピンセット等の工具28を挿入して下栓22を残した状態で取外すことができるため、ダニが飼育容器10から逃走するのを防止することができる。下栓22は、そのまま飼育容器10の上蓋として機能する。すなわち、飼育空間11aの通気と、ダニの逃走を防止する。
【0039】
第3のステップとして、飼育容器10内で所定期間ダニ等を飼育する。ダニは飼育容器10内の給水タンク13からの水分を受けて適度な湿気を有する飼育空間11a内で清潔な状態で成長する。最初に体長が0.8mm程度の幼ダニは、約4週間以上飼育した後、飽血させると脱皮して若ダニとなり、さらに飼育して飽血させると脱皮して成ダニとなり、体長が3mm程度となって、目視で容易に確認できる程度に成長する。
【0040】
第4のステップとして、第3のステップにおいて必要に応じてダニの飼育環境を清潔に保つため、飼育容器10を交換する。この場合、底栓14を取去り、給水タンク13を外してピストン16を円筒状ケース11の下方開口から挿入し、ダニ床12ごと円筒状ケース11の上方開口から取出すようにする。そして、例えば吸引器を使用して、新しいダニ床をセットした洗浄済みの円筒状ケース11内、あるいは新しい飼育容器10内にダニを入れる。
【0041】
第5のステップとして、飼育容器10で飼育され成長したダニ等を産卵容器である産卵ベッド30に入れ替える。飼育容器10の底栓14を取去り、給水タンク13を取外して円筒状ケース11内に下方からピストン16を挿入する。ピストン16を押上げるとダニ床12が上昇し、ダニはダニ床12と共に上部に移動して上蓋15又は下栓22に挟まれた状態で円筒状ケース11外に出される。このようにして、ピストン16で容易に飼育容器10から出されたダニは、十分に飽血させたあと、産卵ベッド30の各産卵スペース35に1匹ずつ入れられる。飽血したダニは体長が5〜10mm程度となり、産卵スペース35内で数千個の産卵を行う。産卵スペース35は上ケース33の突部33aでダニの移動が阻止されており、個別の継代管理が確実に行える。
【0042】
このように、前記したダニ等の微小生物の飼育方法では、卵から幼ダニ、幼ダニから若ダニを経て成ダニになるまで、安全に、迅速に管理することができる。また、成長した成ダニを飽血させて産卵ベッドで個別管理して産卵させることができ、ダニの個別継代と飽血成ダニの集約的な管理が容易に行え、かつ省スペースで管理することができる。
【0043】
以上、本発明の実施形態について詳述したが、本発明は、前記の実施形態に限定されるものではなく、特許請求の範囲に記載された本発明の精神を逸脱しない範囲で、種々の設計変更を行うことができるものである。例えば、円柱状の飼育容器において、給水手段として給水タンクの例を示したが、水を湿らせた綿を円筒状ケース内に入れて乾燥を防止するようにしてもよい。この場合、綿が乾燥したときは底栓を外して綿を取出し、水分を補給すればよい。また、吸引ジョイントのホルダの切込みは、複数個所に設けるようにしてもよい。なお、微小生物として、ダニの例を示したが、ダニに限らず蟻や蜘蛛等のような微小生物にも適用できることは勿論である。
【0044】
さらに、産卵ベッド30の上ケース33に突部33aを設けて、ダニの移動を阻止するように構成したが、円周壁部32dの上端が上ケース33の内面に接するようにして、ダニの移動を阻止するように構成してもよい。また、上ケースの突部は円周壁部の内周側に嵌合するようにしてもよく、上ケースの内面に薄いスポンジ等を貼着してダニが逃走するのを防止してもよい。
【0045】
【発明の効果】
以上の説明から理解できるように、本発明に係るダニ等の微小生物の飼育方法によれば、ダニ等の継代を行う飼育管理が容易に、安全にしかも迅速に行うことができる。
【0046】
容器状の選別器具である選別容器は、ダニ等を不要物から分離して容易に選別することができる。また、円柱状の飼育容器は、円筒状ケース内で給水手段により水分を供給しながらダニ等を飼育でき、円筒状のケースにピストンを挿入して押出すことにより、飼育中のダニ等を一度に容器外に出すことができるので、作業効率が向上する。
【0047】
ダニ等を飼育容器等に入れるとき使用する収集器具である吸引ジョイントは、ダニ等の逃走を防止して、ダニ等を容易に飼育容器に入れることができる。ダニ等を飼育して産卵させる産卵ベッドは、ダニ等を個別に継代管理することができ、集約的に省スペースで産卵管理することができる。
【図面の簡単な説明】
【図1】本発明に係る飼育方法に使用する選別容器の一実施形態の斜視図。
【図2】図1のA−A線断面図。
【図3】(a)は本発明の飼育方法に使用する飼育容器の一実施形態の斜視図、(b)は(a)の飼育容器に使用するピストンの斜視図、(c)は(a)の飼育容器の給水タンクの斜視図。
【図4】図3に示す飼育容器の動作状態を示す斜視図。
【図5】本発明に係る飼育方法に使用する収集器具である吸引ジョイントを示し、(a)は一実施形態の斜視図、(b)は(a)の縦断面図、(c)は他の実施形態の斜視図。
【図6】図5に示す吸引ジョイントの動作状態を示す要部断面図。
【図7】本発明に係る飼育方法に使用する産卵ベッドの一実施形態の一部を破断した分解状態の斜視図。
【図8】図7の産卵ベッドの一部を組み立てた状態のB−B線断面図。
【符号の説明】
1 選別容器(飼育器具)、
2 底壁、        3 外周壁、
4 内周壁、       5 隔離部、
6 スポンジ(吸水性素材)、
10 飼育容器、     11 円筒状ケース、
11a 飼育空間、
12 ダニ床(飼育床)、 13 給水タンク、
14 底栓、       15 上蓋、
16 ピストン、
20 吸引ジョイント(収集器具)、
21,21A ホルダ、  22 下栓、
23,23A 上栓、   24 内部空間、
25,25A 切込み、
26,27 吸引パイプ、
30 産卵ベッド(産卵容器)、
31 下ケース、
32 中間ホルダ部、   32d 円周壁部、
33 上ケース(蓋体)、
35 産卵スペース(複数の区画)、
36 貫通孔、      S 空間(給水区画)
[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a breeding method and a breeding container for subculturing and managing microscopic organisms such as mites, and in particular, a container-like sorting device for selecting ticks and the like, a breeding container that can easily take out mites and the like out of the container. The present invention relates to a collection device for collecting ticks and the like in a breeding container, a container for laying eggs such as ticks, and a breeding method using these sorting devices, breeding containers and the like.
[0002]
[Prior art]
Conventionally, as a tool to subculture and breed this kind of small creatures such as mites, put a wet cotton in a large test tube, lay a sponge on it, put a tick on it By attaching a cotton plug, the container was used as a tick breeding container or an egg-laying container. In addition, when exchanging the breeding container and attaching the mites by the ear bag method, the mites were gradually removed from the container into bats or ear bags. Here, the tick attachment by the ear bag method is to put a tick in a cloth bag or the like and attach it to the ears of livestock or the like to cause blood loss.
[0003]
[Problems to be solved by the invention]
By the way, in the method of breeding by subculture management with the breeding equipment as described above, ticks are vulnerable to drying and mold, and therefore, it is necessary to prevent drying and prevent the occurrence of mold due to shells and excrement due to the removal of mites in the breeding container. Therefore, it is necessary to appropriately replace the breeding container with a new breeding container. However, there is a problem that it is difficult to remove mites attached to the breeding container and the working efficiency is low.
[0004]
In addition, when sucking and collecting ticks into a new breeding container, it is necessary to temporarily remove the ticks from the container, but there is a problem in that the ticks are highly likely to escape due to escape from the clouds. A suction device is used for sucking and collecting ticks. However, there is a problem in that the ticks gather at the bottom of the suction joint connected to a container such as a test tube, and there is a high possibility that the ticks are escaped when the suction joint and the stopper are replaced. Furthermore, in order to carry out the systematic passage of ticks, it is necessary to put one saturating adult mite in each breeding container and manage it, and there is a problem that the breeding space is increased.
[0005]
The present invention has been made in view of such a problem, and an object of the present invention is to easily, safely, and quickly carry out systematic subculture management work of microscopic organisms such as mites, An object of the present invention is to provide a breeding method that can efficiently breed mites and the like. It is another object of the present invention to provide an instrument for selecting and collecting microscopic organisms such as mites used in the above-described breeding method, and a breeding container and an egg-laying container capable of breeding efficiently.
[0006]
[Means for Solving the Problems]
In order to achieve the above object, the method of breeding small creatures such as ticks according to the present invention comprises collecting small creatures such as hatched ticks by spontaneously moving them from unnecessary materials such as dead bodies by placing them in a sorting device, The collected micro-organisms are bred in a breeding container for a predetermined period of time, and the grown micro-organisms are laid in a spawning container having a plurality of spawning spaces arranged side by side. For the sorting of ticks and the like, a tick or the like is put into the center of a sorting tool having a wall portion containing water on the outer periphery with a container-like sorting tool and separated from unnecessary substances such as dead bodies. The collection of mites or the like may be carried out by directly sucking them into the incubator using a suction device or the like, or may be collected using a dropper or the like and put in the incubator.
[0007]
According to this breeding method, ticks have a property of avoiding moisture while being cloudy, so that they can be easily separated from dead matter such as dead bodies, excreta, and shed shells in the sorting container. In addition, the breeding container can be easily sucked and put into the breeding container, and the breeding container can be easily replaced for the purpose of preventing contamination. Therefore, the breeding container is bred in a clean environment, and can be prevented from weakening or dying. The ticks and the like that have been bred and grown for a predetermined period are taken out of the breeding container and put into an egg-laying container, so that the egg-laying can be intensively managed in a small space. In this way, it is easy to sort out ticks, replace breeding containers, and move them to egg-laying containers, so that work efficiency is good, the inside of breeding containers can be kept clean, and the passage control of ticks etc. can be done safely and quickly. Can be done.
[0008]
Further, the sorting device according to the present invention is used in the breeding method described above, is a container-like sorting device that separates small creatures such as mites from unnecessary matters such as dead bodies, and has a space in the center, It is characterized in that it has a wall portion containing a substance for avoiding microscopic organisms on the outer peripheral portion. As a substance that avoids microscopic organisms, substances such as water and alcohol that are not preferred by microscopic organisms such as mites are used. This sorting device is preferably a white and flat circular container, and it is preferable that a water-absorbing material such as a sponge is inserted between the outer peripheral wall and the inner peripheral wall to form a wall containing moisture.
[0009]
According to the sorting device configured in this way, when a large number of ticks during breeding are put into the central space, the ticks move in clouds in all directions, and when approaching the wall containing moisture on the outer circumference, the moisture is removed. Avoid and retreat. For this reason, mites can be easily selected and separated from the dead body, excrement, shed shells and the like in the central portion, and escape can be prevented. When the temperature is high, the mites move around actively.However, when the temperature is low, the activities are suppressed or stopped, so the moisture and the like on the outer peripheral wall are cooled by using the sorting device of the present invention under cooling, so that the mites move. Therefore, the mites can be further efficiently separated and separated from unnecessary substances. In addition, when alcohol is appropriately contained in the wall, the alcohol evaporates to remove heat from the outer peripheral wall, thereby effectively isolating mites by the effect of lowering the temperature of the wall. Since alcohol is harmful to mites, it is preferable to use it at the lowest possible concentration.
[0010]
The breeding container according to the present invention is used in the breeding method described above, and is a breeding container for breeding small creatures such as ticks, and has a cylindrical case and a bottom for closing an opening at a lower end of the cylindrical case. A stopper, a water supply means located above the bottom stopper, a breeding floor located above the water supply means and supplied with water by the water supply means, and an upper lid for closing an upper opening of the cylindrical case. It is characterized by: As the water supply means, a water supply tank for storing water, cotton containing water, or the like can be used. The upper lid is preferably made of a sponge or the like that has air permeability and prevents escape of mites and the like. The breeding container preferably further includes a piston which is inserted from below into the cylindrical case with the bottom plug and the water supply means removed, and which can push up the breeding floor and move out of the cylindrical case.
[0011]
According to this breeding container, it is possible to easily supply water while the mite remains in the container, and it is possible to keep the inside of the container at an appropriate humidity, so that breeding management of ticks and the like can be easily performed. it can. Although the ticks during breeding are attached to the inner wall of the cylindrical case, the breeding floor can be pushed up by using a piston and taken out of the container at a time, and the cylindrical case can be easily cleaned.
[0012]
The collection device according to the present invention is a collection device that is attached to the cylindrical case of the breeding container and collects small organisms, and has a wall that can be inserted into the cylindrical case and has a downwardly opened holder, A lower plug that closes the lower opening of the holder to form an internal space; an upper plug that is connected to the upper part of the holder to close the opening of the cylindrical case; and a lower plug that is formed from below on the wall surface of the holder to reach the inner space. And a pipe penetrating the upper and lower plugs and a pipe penetrating the upper plug.
[0013]
According to the configuration of this collecting instrument, when placing the ticks separated by spontaneous movement from unnecessary objects into the breeding container in the sorting container for sorting the ticks and the like, a suction device is connected to a pipe penetrating the upper stopper, When a tube or the like connected to a pipe penetrating the lower stopper is brought close to the mites, the mites are inserted into the upper space of the breeding floor of the breeding container through a pipe penetrating to the lower stopper via the tube, and a collecting device. The collecting device can be removed from the cylindrical case while holding the lower stopper in the cylindrical case by inserting a tool such as tweezers into the notch of the holder, thereby preventing the mites from escaping out of the breeding container. .
[0014]
Further, the egg-laying container according to the present invention is used in the above-described breeding method, and is an egg-laying container for laying small creatures such as ticks, wherein a plurality of compartments having upper openings arranged side by side in the horizontal direction, , A water supply section located at a lower part of the section, a through hole communicating the water supply section with each of the plurality of sections, and a lid closing an upper opening of the plurality of sections. The plurality of sections can be selected as appropriate, such as those arranged in one row, those arranged vertically and horizontally. It is preferable to insert gauze or the like into the plurality of compartments by pushing gauze or the like as a mesh material having an upper opening.
[0015]
The spawning container of the present invention configured as described above puts the blood-saturated ticks that have grown and sucked enough blood into the spawning space, which is a plurality of compartments of the upper opening arranged side by side in the horizontal direction, and the entire spawning space. Can be checked at a glance and can be managed collectively in a small space. In addition, since the vaporized water is appropriately supplied to the egg-laying space from the water supply section located at the lower portion through the through-hole, the environment for egg-laying is prepared, and individual passage of ticks can be easily managed. When gauze or the like is inserted into the egg-laying space, ticks tend to rise and prevent the mites from rolling over, so that the ticks and the like can be prevented from weakening and eggs can be laid quickly.
[0016]
BEST MODE FOR CARRYING OUT THE INVENTION
Hereinafter, as a breeding container and a sorting device used in the method for breeding small creatures such as mites according to the present invention, first, an embodiment of a sorting container as a container-like device for sorting ticks will be described in detail based on the drawings. . FIG. 1 is a perspective view of a sorting container according to the present embodiment, and FIG. 2 is a sectional view taken along line AA of FIG.
[0017]
In FIGS. 1 and 2, the sorting container 1 temporarily isolates mites in the container by utilizing the property of mites to avoid water when sorting and collecting microscopic organisms such as Tickle mite. The sorting container 1 has a container shape composed of a bottom wall 2 and an outer peripheral wall 3 made of plastic or metal. The inner peripheral wall 4 has a predetermined distance from the outer peripheral wall 3 to the inside. Is formed, and the height of the inner peripheral wall 4 is set smaller than that of the outer peripheral wall 3. The space inside the inner peripheral wall 4 serves as a mite isolation part 5. If the sorting container 1 is formed in a circular flat container shape from, for example, a white synthetic resin, it is convenient to easily find mites.
[0018]
The outer peripheral side of the mite isolation part 5 is a ring-shaped space sandwiched between the outer peripheral wall 3 and the inner peripheral wall 4, and a ring-shaped sponge 6 is fitted into this space as a water-absorbing material. The sponge 6 is sufficiently hydrated when used. As described above, the sorting container 1 has the space of the separating part 5 in the center part, and the ring-shaped sponge 6 containing water is located on the outer peripheral part to form a wall. The sponge 6 may contain an alcohol having a property to avoid mites.
[0019]
The height of the sponge 6 is lower than the outer peripheral wall 3 and higher than the inner peripheral wall 4. The inner peripheral wall 4 has a certain height in order to prevent the mites from climbing up their backs and forming a bridge to escape. The water-absorbing material is not limited to a sponge, and any material such as cotton or glass wool may be used as long as it sufficiently contains water. The sponge 6 preferably has a protruding portion 6a that covers the upper end surface of the inner peripheral wall 4 as shown in FIG.
[0020]
The operation of the sorting container 1 for sorting ticks and the like according to the present embodiment configured as described above will be described below. When breeding ticks are placed in the isolation part 5 at the center of the sorting container 1, living ticks, mite carcasses, excreta of the ticks, shed shells and the like are concentrated in the center. Then, as time elapses, the mites escape in all directions, leaving only unnecessary objects in the center. When the mite that has climbed the inner peripheral wall 4 touches the sponge 6 that has absorbed water, it retreats and returns to the bottom surface, so that living mites gather around the bottom surface and are separated by spontaneous movement. For this reason, by sucking this part, the experimental mites can be separated and collected, and can be sorted out from unnecessary substances.
[0021]
Next, an example of a cylindrical rearing container as a rearing container for mites or the like will be described with reference to FIGS. The breeding container 10 includes a transparent or translucent cylindrical case 11, and a cylindrical mite floor 12 is fitted to a center of the cylindrical case 11 as a breeding floor formed of a sponge or the like. A water supply tank 13 is inserted as a water supply means so as to be in contact therewith, a bottom plug 14 supporting the water supply tank 13 is fitted and fixed to a lower end of the cylindrical case 11, and a sponge or the like is formed at an upper end of the cylindrical case 11. The air-permeable top cover 15 is fitted, and the space between the top cover 15 and the tick floor 12 is a breeding space 11a. The mite floor 12 serves to supply moisture to the breeding space 11a to prevent the mite from drying, and to receive the mites, dead mites and shells.
[0022]
As shown in FIG. 3 (c), the water supply tank 13 includes a water supply container 13a having an outer diameter that is loosely inserted into the cylindrical case 11, and a water absorption wick 13b fitted into an upper opening of the water supply container. You. The water absorption wick 13b is for sucking up water in the water supply container and supplying water to the tick floor 12 of the sponge. The upper portion is a large diameter portion fitted to the inner periphery of the water supply container 13a. A small-diameter portion 13c is provided which hangs downward from above and absorbs water, and is entirely formed of a water-absorbing material such as a sponge.
[0023]
The bottom stopper 14 is formed of a rubber stopper having a truncated cone shape, and is firmly fitted to the lower opening of the cylindrical case 11 to prevent the water supply tank 13 from dropping. The upper lid 15 is formed of sponge, cotton, or the like, and is configured to transmit air to the ticks breeding on the tick floor 12 and, when the ticks climb the inner surface of the cylindrical case 11, the cylindrical case 11 Prevents escape from inside.
[0024]
The breeding container 10 further includes a piston 16 inserted into the cylindrical case 11 shown in FIG. The upper part of the piston 16 is a push-up part 16a that fits loosely on the inner surface of the cylindrical case 11, the lower part is an operation part 16b that is pressed by a finger, and the middle part is a connection shaft part 16c. The piston 16 is removed from the bottom case 14 and the water supply tank 13 and inserted into the cylindrical case 11 from below. The piston 16 pushes up the tick floor 12 and moves out of the cylindrical case 11 as shown in FIG. It goes out. By removing the bottom plug 14 and the water supply tank 13 of the breeding container 10 and inserting the piston 16, the breeding container 10 becomes an injection type.
[0025]
Since the cylindrical rearing container 10 can be supplied with water by removing the bottom plug 14 and removing the water supply tank 13 while rearing the mites on the tick floor 12, the rearing management of the mites can be easily performed. Further, the structure is simple, and when all of them are removed, only the cylindrical case 11 is provided, so that cleaning is easy and cleanliness can be maintained. Then, when the mites during rearing are moved, the bottom plug 14 is removed, the water supply tank 13 is pulled out, the piston 16 is inserted from below, the mite floor 12 is pushed up, and the mites can be easily moved by being pushed out together with the upper lid 15. .
[0026]
By winding or wrapping the side or entire surface of the mite floor 12 with a net (not shown) made of a slippery material such as polyethylene, the tick floor can be smoothly extruded with reduced frictional resistance when sliding. That is, if the cylindrical case 11 is made of glass, the inner wall surface will be finely scratched by washing, and if the tick bed 12 is pushed up by the piston 16, friction will occur between the inner wall surface and the tick floor, and It is possible to prevent the floor from being twisted or the like, so that the mite floor 12 cannot be pushed out smoothly.
[0027]
As an embodiment of the collecting device according to the present invention, an example of a suction joint 20 attached to a cylindrical case 11 for putting a tick into a breeding container 10 shown in FIG. 3 will be described with reference to FIGS. The suction joint 20 is a joint device for efficiently collecting and placing ticks and the like in the injection-type breeding container 10. The suction joint 20 has a cylindrical wall having an outer diameter that can be inserted into the cylindrical case 11 of the breeding container 10. A container-like holder 21 having a downward opening, a lower stopper 22 formed of a sponge or the like closing the lower opening of the holder 21 and functioning as a temporary plug, a rubber stopper connected to an upper portion of the holder 21 by a set screw 21a, and the like. The upper stopper 23 is provided. One set screw 21a may be provided at the center of the holder 21, or two or more set screws may be provided on the circumference.
[0028]
The upper stopper 23 has an inverted truncated cone shape, and the outer diameter of the upper end is formed larger than the inner diameter of the cylindrical case 11 of the breeding container 10. The lower stopper 22 has a truncated cone shape, and the diameter of the lower end is formed larger than the inner diameter of the cylindrical case 11 of the breeding container 10. The lower stopper is not limited to a truncated cone, but may be formed in a columnar shape. In this case, the outer diameter of the lower stopper is formed to be larger than the inner diameter of the cylindrical case, and the lower stopper is fitted to the inner periphery of the holder and is brought into close contact with the inner wall of the cylindrical case to close the gap.
[0029]
The holder 21 is formed of transparent plastic, metal, or the like, and the lower opening is closed by a lower stopper 22 to form an internal space 24. Two cuts 25 are formed in the outer peripheral surface of the holder 21 in the axial direction from below, and the cuts 25 are formed so as to reach the internal space 24. For this reason, a tool such as tweezers can be inserted into the internal space 24 avoiding the lower stopper 22. A long suction pipe 26 penetrating the upper plug 23 also penetrates the lower plug 22. Another short suction pipe 27 penetrating through the upper plug 23 opens into the internal space 24. The suction joint 20 functions as a suction joint of a suction device (not shown) that sucks and collects mites and the like into the breeding container 10.
[0030]
The suction joint may be formed by forming the holder 21A from a cylinder and fitting the upper plug 23A to the upper opening of the holder 21A and connecting the holder 21A like a suction joint 20A shown in FIG. 5C. In this example, the number of cuts 25A formed in the holder 21A is one, but it is needless to say that two may be used.
[0031]
When the suction joint 20 is used, the upper lid 15 of the breeding container 10 shown in FIG. 3 is removed, and the lower plug 22 and the holder 21 of the suction joint 20 are opened through the upper opening of the cylindrical case 11 as shown in FIG. And the upper plug 23 is fitted. Then, an aspirator (not shown) is connected to the suction pipe 27 of the upper plug 23 by a connecting pipe, and the mites are sucked by the tube connected to the suction pipe 26. As a result, the mites are connected to the tick bed 12 and the lower plug 22 as shown by an arrow Y1. And the exhaust can be discharged as shown by an arrow Y2. When the mites are sucked into the breeding container, the water supply tank 13 is removed, and after the mites are suctioned and collected, the water supply tank 13 is inserted, and the bottom stopper 14 is fitted and fixed to the cylindrical case 11.
[0032]
After the ticks are collected by suction and placed in the breeding space 11a, the suction joint 20 is pulled up to a position where the lower stopper 22 coincides with the upper end of the cylindrical case 11, as shown in FIG. Then, a tool 28 such as tweezers is inserted into the internal space 24 through the cut 25, and the upper portion of the suction joint 20 is pulled out from the holder 21 while pressing the lower stopper 22 to prevent the movement. As a result, as shown in FIG. 6C, since the lower plug 22 remains in the cylindrical case 11, the mites are blocked by the lower plug 22 and are prevented from moving upward, so that the mites can be prevented from escaping. Since the lower stopper 22 is formed of a sponge, the hole 26a from which the suction pipe 26 penetrating through the lower stopper 22 is naturally closed by the restoring force, so that the mites can be prevented from escaping.
[0033]
As an embodiment of the egg-laying container of the present invention, an example of an egg-laying bed on which mites lay eggs will be described with reference to FIGS. The spawning bed 30 has a rectangular lower case 31 opening upward, an intermediate holder portion 32 detachably fitted into the opening of the lower case 31 to form a plurality of sections, and an upper case covering an upper portion of the intermediate holder portion. 33. The lower case 31 stores water for supplying water to the mites bred for spawning, and is formed of, for example, a rectangular plastic case.
[0034]
The intermediate holder portion 32 includes an outer peripheral wall portion 32a fitted inside the lower case 31, a bottom wall portion 32b forming an inner bottom surface of the outer peripheral wall portion, and a flange portion 32c extending outside the outer peripheral wall portion. When the flange portion 32c is in contact with the upper end of the lower case 31, a space S that is a water supply section is formed between the bottom wall portion 32b and the bottom surface of the lower case 31. A plurality of partitions used as the spawning space 35 are formed by the circumferential wall portion 32d extending upward from the bottom wall portion 32b, and in the illustrated example, 24 circumferential wall portions are formed. For example, one section located at the corner has a bottom wall portion penetrating therethrough, and serves as a water supply section 34 to the space S of the lower case 31. A through-hole 36 is formed in the center of the bottom wall 32b of each section, and the space S of the lower case 31 and the plurality of egg laying spaces 35 communicate with each other. On the lower surface of the upper case 33, a circumferential projection 33a fitted to the outer periphery of the circumferential wall portion 32d is formed to prevent the mites from escaping upward and moving to the adjacent section.
[0035]
A mesh material 37 is spread over the plurality of egg-laying spaces 35 to support the rising of ticks and prevent the ticks from rolling over. The mesh material 37 is formed, for example, by pressing gauze with a finger to form a container having an open top, but is not limited to this, and may be a container formed of synthetic resin into a mesh. In the egg-laying space 35, water supplied to the lower case 31 is evaporated and rises from the through-hole 36 to maintain an appropriate amount of water, which provides an environment suitable for egg-laying. Since the plurality of spawning spaces 35 are arranged horizontally and vertically, the mite situation and the spawning situation can be easily grasped at a glance.
[0036]
In the method for breeding small creatures such as ticks according to the present invention, as a first step, small creatures such as ticks are separated from unnecessary substances and sorted using the sorting container 1 shown in FIGS. In other words, mites weaken and die during breeding, husks scatter shedding shells, and are covered with excrement and become dirty. Live mites are screened from such a filthy environment. For example, when a tick kept in a test tube is put into the center of the sorting container 1, the tick becomes cloudy with time, and the fast-moving tick moves up the inner peripheral wall 4 and approaches the sponge 6 containing water. However, since the mites have a property of avoiding water, when they come into contact with the sponge 6, they retreat and separate and concentrate on the outer peripheral portion of the bottom surface of the sorting container 1 by spontaneous movement. As described above, the mite may be separated by including a low concentration of alcohol in the sponge 6 as the wall.
[0037]
As a second step, the mites separated on the outer periphery of the sorting container 1 are put into a column-shaped rearing container 10. At this time, the suction joint 20 is used as a collecting device that is connected to the upper opening of the breeding container 10 to suck and collect the mites. The upper stopper 23 of the suction joint 20 is fitted into the upper opening of the cylindrical case 11 of the breeding container 10, a short suction pipe 27 is connected to a suction device such as an electric suction pump, and the tube connected to the long suction pipe 26 is separated from the sorting container 1. Approach isolated ticks. When the suction device is operated, the mites are sucked into the breeding space 11a of the breeding container 10 through the tube, and the exhaust air is discharged from the short pipe through the suction device. Note that a filter may be provided in the middle of the suction pipe 27 to prevent the mites from being sucked into the suction device.
[0038]
After the mites have been inhaled into the breeding container 10, the suction joint 20 is removed from the breeding container 10. However, as described above, the suction joint 20 is pulled up, a tool 28 such as tweezers is inserted into the cut 25, and the lower stopper 22 is left. The mites can be prevented from escaping from the breeding container 10 because they can be removed in a state where the ticks are removed. The lower stopper 22 functions as the upper lid of the breeding container 10 as it is. That is, ventilation of the breeding space 11a and escape of mites are prevented.
[0039]
As a third step, ticks and the like are bred in the breeding container 10 for a predetermined period. The mites receive moisture from the water supply tank 13 in the breeding container 10 and grow in a clean state in the breeding space 11a having appropriate humidity. First, a young mite with a body length of about 0.8 mm is bred for about 4 weeks or more, and then molted and becomes a young tick when saturated, and when bred and saturated, it molts and becomes an adult mite and has a body length of 3 mm. And grow to such an extent that it can be easily confirmed visually.
[0040]
As a fourth step, the breeding container 10 is replaced as necessary in the third step in order to keep the breeding environment of the mites clean. In this case, the bottom plug 14 is removed, the water supply tank 13 is removed, the piston 16 is inserted from the lower opening of the cylindrical case 11, and the mite floor 12 is taken out from the upper opening of the cylindrical case 11. Then, using a suction device, for example, the mites are placed in the cleaned cylindrical case 11 in which a new mite bed is set, or in the new breeding container 10.
[0041]
As a fifth step, mites and the like raised and raised in the breeding container 10 are replaced with an egg-laying bed 30 which is an egg-laying container. The bottom plug 14 of the breeding container 10 is removed, the water supply tank 13 is removed, and the piston 16 is inserted into the cylindrical case 11 from below. When the piston 16 is pushed up, the tick bed 12 rises, and the tick moves upward together with the tick bed 12 and is taken out of the cylindrical case 11 while being sandwiched by the upper lid 15 or the lower stopper 22. In this manner, the mites easily taken out of the breeding container 10 by the piston 16 are sufficiently saturated and then put into each of the spawning spaces 35 of the spawning bed 30 one by one. Saturated mites have a body length of about 5 to 10 mm, and lay eggs in the spawning space 35 in thousands. In the spawning space 35, the movement of mites is prevented by the projections 33a of the upper case 33, so that individual passage management can be performed reliably.
[0042]
As described above, according to the above-described method for breeding small creatures such as ticks, it is possible to safely and quickly manage from eggs to young ticks, from young ticks to young ticks to adult ticks. In addition, mature adult mites can be engorged and individually managed and spawned in the spawning bed. Individual passage of mites and intensive management of adult mites can be easily performed, and space-saving management is possible. be able to.
[0043]
As described above, the embodiments of the present invention have been described in detail. However, the present invention is not limited to the above embodiments, and various designs may be made without departing from the spirit of the present invention described in the appended claims. Changes can be made. For example, in the column-shaped breeding container, an example of a water supply tank is shown as a water supply means, but it is also possible to prevent water from being dried by putting cotton moistened with water in a cylindrical case. In this case, when the cotton has dried, the bottom plug is removed, the cotton is taken out, and water may be supplied. The cuts of the holder of the suction joint may be provided at a plurality of locations. Although an example of a tick is shown as a small creature, it is needless to say that the present invention can be applied not only to a tick but also to a small creature such as an ant or a spider.
[0044]
Furthermore, the projection 33a is provided on the upper case 33 of the spawning bed 30 to prevent the movement of the mites, but the upper end of the circumferential wall 32d is in contact with the inner surface of the upper case 33 so that the movement of the mites is prevented. May be prevented. Further, the protrusion of the upper case may be fitted to the inner peripheral side of the circumferential wall portion, or a thin sponge or the like may be attached to the inner surface of the upper case to prevent the mites from escaping.
[0045]
【The invention's effect】
As can be understood from the above description, according to the method for breeding small creatures such as ticks according to the present invention, breeding management for passaging ticks and the like can be performed easily, safely and quickly.
[0046]
The sorting container, which is a container-like sorting device, can easily sort out ticks and the like by separating them from unnecessary substances. In addition, the cylindrical rearing container can breed ticks and the like while supplying water by a water supply means in a cylindrical case, and by inserting a piston into the cylindrical case and pushing it out, the ticks and the like during breeding can be once removed. Work efficiency can be improved.
[0047]
The suction joint, which is a collecting instrument used when placing ticks and the like in a breeding container or the like, can prevent the ticks or the like from escaping and easily put the ticks or the like in the breeding container. The spawning bed for rearing and laying ticks and the like can individually manage ticks and the like, and can collectively manage eggs in a space-saving manner.
[Brief description of the drawings]
FIG. 1 is a perspective view of one embodiment of a sorting container used in a breeding method according to the present invention.
FIG. 2 is a sectional view taken along line AA of FIG.
3A is a perspective view of an embodiment of a breeding container used in the breeding method of the present invention, FIG. 3B is a perspective view of a piston used in the breeding container of FIG. 3A, and FIG. The perspective view of the water supply tank of the breeding container of ()).
FIG. 4 is a perspective view showing an operation state of the breeding container shown in FIG. 3;
5A and 5B show a suction joint which is a collecting instrument used in the breeding method according to the present invention, wherein FIG. 5A is a perspective view of one embodiment, FIG. 5B is a longitudinal sectional view of FIG. FIG.
FIG. 6 is a sectional view of a main part showing an operation state of the suction joint shown in FIG. 5;
FIG. 7 is a perspective view of an exploded state in which a part of an embodiment of the spawning bed used in the breeding method according to the present invention is broken.
8 is a sectional view taken along the line BB of a state where a part of the egg-laying bed of FIG. 7 is assembled.
[Explanation of symbols]
1 sorting container (breeding equipment),
2 bottom wall, 3 outer wall,
4 inner wall, 5 isolation part,
6 sponge (water absorbing material),
10 rearing container, 11 cylindrical case,
11a breeding space,
12 mite floor (breeding floor), 13 water tank,
14 bottom stopper, 15 top lid,
16 pistons,
20 suction joints (collection instruments)
21, 21A holder, 22 lower stopper,
23, 23A top plug, 24 internal space,
25,25A cut,
26,27 suction pipe,
30 spawning beds (spawning containers),
31 Lower case,
32 intermediate holder part, 32d circumferential wall part,
33 upper case (lid),
35 spawning space (multiple compartments),
36 through hole, S space (water supply section)

Claims (6)

孵化したダニ等の微小生物を選別器具に入れて死骸等の不要物から自発的移動によって分離させ、分離された前記微小生物を飼育容器内に収集し、収集された前記微小生物を前記飼育容器に所定期間入れて飼育し、成長した前記微小生物を複数の産卵スペースを並設した産卵容器に入れて産卵させることを特徴とするダニ等の微小生物の飼育方法。The hatched ticks and other small creatures are put into a sorting device and separated by spontaneous movement from unnecessary substances such as dead bodies, and the separated tiny creatures are collected in a breeding container. A method of breeding micro organisms such as mites, wherein the creatures are bred for a predetermined period, and the grown micro organisms are laid in an egg-laying container provided with a plurality of egg-laying spaces. ダニ等の微小生物を死骸等の不要物から分離させる容器状の選別器具であって、
中央部に空間を有し、外周部に前記微小生物を回避させる物質を含んだ壁部を有することを特徴とするダニ等の微小生物を分離させる選別器具。
A container-like sorting device that separates small creatures such as mites from unnecessary materials such as dead bodies,
A sorter for separating microbes such as ticks, characterized by having a space in the center and a wall containing a substance for avoiding the microbes on the outer periphery.
ダニ等の微小生物を飼育する飼育容器であって、
円筒状ケースと、該円筒状ケースの下端の開口部を閉塞する底栓と、該底栓の上部に位置する給水手段と、該給水手段の上部に位置して該給水手段により水分を供給される飼育床と、前記円筒状ケースの上部開口を閉塞する上蓋とを備えることを特徴するダニ等の微小生物の飼育容器。
A breeding container for breeding small creatures such as mites,
A cylindrical case, a bottom plug that closes an opening at a lower end of the cylindrical case, a water supply unit located above the bottom plug, and water supplied by the water supply unit located above the water supply unit. A breeding container for a small creature such as a tick, comprising: a breeding floor; and an upper lid for closing an upper opening of the cylindrical case.
前記底栓及び給水手段を外して前記円筒状ケース内に下方から挿入され、前記飼育床を押上げて前記円筒状ケース外に移動可能のピストンをさらに備える請求項3に記載のダニ等の微小生物の飼育容器。4. The microscopic device according to claim 3, further comprising a piston which is inserted into the cylindrical case from below by removing the bottom plug and the water supply means, and is capable of pushing up the breeding floor and moving out of the cylindrical case. A breeding container for living things. 前記請求項3に記載の飼育容器の円筒状ケースに装着され微小生物を収集する器具であって、
前記円筒状ケース内に挿入可能の壁面を有し下方に開口するホルダと、該ホルダの前記開口を閉塞して内部空間を形成する下栓と、前記ホルダの上部に連結され前記円筒状ケースの開口を閉塞する上栓と、前記ホルダの壁面に下方から形成され前記内部空間に至るように形成された切込みと、前記上栓及び下栓を貫通するパイプ及び前記上栓を貫通するパイプとを備えることを特徴とする飼育容器の収集器具。
It is a device attached to a cylindrical case of the breeding container according to the claim 3 for collecting micro organisms,
A holder that has a wall surface that can be inserted into the cylindrical case and that opens downward, a lower plug that closes the opening of the holder to form an internal space, and a cylindrical plug that is connected to an upper part of the holder and An upper plug for closing the opening, a cut formed on the wall surface of the holder from below to reach the internal space, a pipe passing through the upper plug and the lower plug, and a pipe passing through the upper plug. A collection device for breeding containers, comprising:
ダニ等の微小生物が産卵する産卵容器であって、
水平方向に並設した上方開口の複数の区画と、該複数の区画の下部に位置する給水区画と、該給水区画と前記複数の区画のそれぞれとを連通する貫通孔と、前記複数の区画の上方開口を閉塞する蓋体とを備えることを特徴とするダニ等の微小生物の産卵容器。
An egg-laying container in which small creatures such as mites lay eggs,
A plurality of sections of an upper opening arranged side by side in a horizontal direction, a water supply section located below the plurality of sections, a through-hole communicating the water supply section with each of the plurality of sections, A container for laying small creatures such as mites, comprising a lid for closing an upper opening.
JP2002278978A 2002-09-25 2002-09-25 Breeding methods for micro-organisms such as ticks, sorting equipment, breeding containers, collection equipment for breeding containers and egg-laying containers Expired - Lifetime JP3680135B2 (en)

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011229462A (en) * 2010-04-28 2011-11-17 Mamoru Takada Ant rearing apparatus
KR101351847B1 (en) 2012-11-13 2014-01-22 구경본 Apparatus for capturing natural enemy of mites and method for capturing and formulating natural enemy of mites using the same
KR101477166B1 (en) * 2013-03-25 2014-12-29 오승재 Queen bee trapping device
CN105109844A (en) * 2015-08-20 2015-12-02 罗丽秀 Packaging bottle applicable to live insect conveying
CN107941591A (en) * 2017-12-28 2018-04-20 贵州大学 Small-sized mite class cleaning device
CN108812561A (en) * 2018-08-10 2018-11-16 安徽国际旅行卫生保健中心 One kind, which efficiently separates, collects acarid device
CN109092693A (en) * 2018-09-22 2018-12-28 中国农业科学院植物保护研究所 A kind of separation method, separator and its application of soil small arthropod
EP4233538A1 (en) * 2019-03-18 2023-08-30 Verily Life Sciences LLC Device and method for compressing insects to load insects in an insect storage and release device
CN117044686A (en) * 2023-09-26 2023-11-14 广东省农业科学院植物保护研究所 Cryptocarya mansoni feeding device and feeding method

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011229462A (en) * 2010-04-28 2011-11-17 Mamoru Takada Ant rearing apparatus
KR101351847B1 (en) 2012-11-13 2014-01-22 구경본 Apparatus for capturing natural enemy of mites and method for capturing and formulating natural enemy of mites using the same
KR101477166B1 (en) * 2013-03-25 2014-12-29 오승재 Queen bee trapping device
CN105109844A (en) * 2015-08-20 2015-12-02 罗丽秀 Packaging bottle applicable to live insect conveying
CN107941591A (en) * 2017-12-28 2018-04-20 贵州大学 Small-sized mite class cleaning device
CN108812561A (en) * 2018-08-10 2018-11-16 安徽国际旅行卫生保健中心 One kind, which efficiently separates, collects acarid device
CN108812561B (en) * 2018-08-10 2023-12-05 安徽国际旅行卫生保健中心 Mite device is collected in high-efficient separation
CN109092693A (en) * 2018-09-22 2018-12-28 中国农业科学院植物保护研究所 A kind of separation method, separator and its application of soil small arthropod
EP4233538A1 (en) * 2019-03-18 2023-08-30 Verily Life Sciences LLC Device and method for compressing insects to load insects in an insect storage and release device
CN117044686A (en) * 2023-09-26 2023-11-14 广东省农业科学院植物保护研究所 Cryptocarya mansoni feeding device and feeding method
CN117044686B (en) * 2023-09-26 2024-01-19 广东省农业科学院植物保护研究所 Cryptocarya mansoni feeding device and feeding method

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