JP2003335656A - Skin care preparation for external use - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation for external use, which controls complicated skin inflammatory reactions, or the like, and has synergistically improved prevention and amelioration effects on chapped skin and skin aging. <P>SOLUTION: This skin care preparation for external use comprises an extract of a plant of the family Salicaceae and one or more kinds of antiinflammatory agents to obtain synergistically improved prevention and amelioration effects on chapped skin and skin aging. A water extract of Salix nigra is preferably used as the extract of the plant of the family Salicaceae. One or more kinds selected from glycyrrhizic acid salt and a derivative thereof, a glycyrrhetic acid salt and a derivative thereof and licorice are preferably used as the antiinflammatory agent. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin in which the effects of preventing and improving skin roughness and skin aging are synergistically improved. More specifically, it relates to an external preparation for the skin, which comprises a combination of Salixaceae ( S0alicaceae ) plant extracts and an anti-inflammatory agent.

[0002]

2. Description of the Related Art Aging, UV exposure, oxidative stress due to reactive oxygen species generated in skin tissue, contact with drugs and various allergens, etc. may cause an inflammatory reaction and aging of the skin. Are known. In the field of external preparations for skin, many physiologically active ingredients such as active oxygen species scavengers, anti-inflammatory agents and anti-allergic agents have been searched and studied in order to prevent such skin inflammation and aging.
Further, due to aging and the stress, as it becomes clear that skin aging symptoms progress due to modification such as cross-linking of collagen which is a dermal matrix component, examination of substances having an action of promoting its production in skin tissues is also conducted. Has been done. In recent years, there is an increasing tendency to seek such ingredients from plants, reflecting the natural and plant orientations of consumers.

However, among the above-mentioned components of plant origin which have already been reported, since the activity is low, it takes a considerably high concentration to obtain a sufficient action and effect when incorporated into an external preparation for skin, and it is stable. Stability and safety when combined with external preparations for skin, as well as effects that may cause unpleasant color and odor to external preparations for skin. The current situation is that there are few things that can be satisfied in all aspects. In addition, the inflammatory reaction and aging of the skin
Since various factors complicatedly participate in the process and proceed, a sufficient effect cannot be obtained even if a substance that acts only in a part of the above-mentioned reaction is used.

[0004]

Therefore, in the present invention, it is intended to obtain a skin external preparation which suppresses complicated inflammatory reaction of skin and the like, prevents skin roughening and skin aging, and synergistically improves the improving effect. Intended.

[0005]

[Means for Solving the Problems] As a result of various studies to solve the above problems, the present inventors found that the Salicaceae family
ae ) The combined use of a plant extract and one or more anti-inflammatory agents in an external preparation for skin can prevent rough skin and skin aging, and obtain synergistic improvement effects. The present invention has been completed and the present invention has been completed.

Regarding the effectiveness of Salicaceae plant extracts, the whitening effect of dihydromyricenan contained in leaves (JP-A-63-316711) and anti-mutagenic effect (JP-A-1-175932), bark extraction It is already known that the histamine release-inhibiting effect of the product (JP-A-10-287582), the fibroblast activation effect of salicin contained in the bark and leaves in large amounts (JP-A-11-80002), and the like.

Anti-inflammatory agents have been included in skin external preparations for a long time in anticipation of the effect of improving rough skin. However, by combining Salicaceae plant extracts with anti-inflammatory agents, The obtained effects of the present invention have never been suggested at all.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION Willow family ( Salica) used in the present invention
ceae ) plants include Chosenia arbutifolia (Pallas) A. Skvortz; Ch.
osenia bracteosa Nakai), cottonwood willow ( porcupine ,
Poplar) the genus of cottonwood (Populus) (Populus siebo
ldi Miq.), Dero ( Populus maxmowiczii He
nry), Populus alba
L.), American Poppy ( Populus deltoides Mars
h .; Populus monilifera Ait .; Populus angulata Ai
v.), populus euphratica (Populus euphratica Oliv.), Europe Black Mountain break-in (Populus nigra L.), Populus nigra (Populus nigra var. italica Koehne) , Europe mountain leveling (Populus tremula), Salix of Kasupiyanagi (Salix acutifolia L.) , European willow
(Salix alba L .; Salix aurea Salisb .), Salix (Itoyanagi) (Salix babylonica L.), wildcat willow (Bakkoyanagi) (Salix bakko Kimura), Akameyanagi (Salix chaenomeloides Kimura), Naga bus sallow
( Salix gilgiana Seemen.), Pussy willow (Enoco willow, river willow) ( Salix gracilistyla Miq.), Dogwood willow ( Salix integra Thunb.), Willow tree ( Salix j
aponica Thunb.), Salix kinuyanagi Kimur
a), Koryanagi ( Salix koriyanagi Kimura; Salix pur
purea var. japonica Nakai), Frisode willow ( Salix l
eucopithecia Kimura), Salix matsu
dana Koidz.), Japanese willow tree
( Salix nakamurana Koidz.), Salix nigr
a ), Salix purpurea L., Salix purpurea L., Salix sachalinensis Fr. Sch
m.), willow tree ( Salix reinii Fr. e)
t Sav.), Salix sieboldiana Bl., Salix viebalis L .; Salix longifolia
Lam.), Fox willow ( Salix vulpin )
a), Oobayanagi genus Oobayanagi (Toisusu urbaniana
Salix urbaniana ), etc. are exemplified. Among these, especially from the viewpoint of its antibacterial action, the European porcupine ( Pop
ulus tremula ), willow ( Salix alba L .; Sa
lix aurea Salisb.), Salix (Itoyanagi) (Sal
i x babylonica L.), Kuroyanagi (Salix nigra), 1 kind selected from purple willow (Salix purpurea L.) or 2
It is preferred to use more than one plant, and most preferred is Salix nigra bark extract. The site for obtaining an extract from a Salicaceae plant is not particularly limited, but a bark, a leaf and a female flower are preferably used in view of its antibacterial action.

Extraction solvents for obtaining the extracts of these Salicaceae plants include water, ethanol, methanol, isopropanol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol,
Alcohols such as n-octyl alcohol, glycerin, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether,
Propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1,3-butylene glycol, hexylene glycol and other polyhydric alcohols or their derivatives, acetone, methyl ethyl ketone, methyl isobutyl ketone, methyl-n-propyl Ketones such as ketones, esters such as ethyl acetate and isopropyl acetate,
One or more mixed solvents selected from polar solvents such as ethers such as ethyl ether, isopropyl ether, n-butyl ether, etc. can be preferably used, and phosphate buffered saline can also be used. However, it is not particularly limited. For the purpose of the present invention, a polar solvent is preferable from the viewpoint of antibacterial activity, and further, one or more mixed solvents selected from methanol, ethanol, 1,3-butylene glycol, and water, particularly water, may be used. It is preferably used as a solvent.

As the extraction method, a method of immersing at room temperature, cooling or heating, extraction using a distillation method such as steam distillation, and a raw Salixaceae
Examples of the method include a pressing method of pressing a plant to obtain an extract, and these methods are used alone or in combination of two or more kinds.

[0011] Without being limited especially Salicaceae (Salicaceae) the ratio of plant and solvent used in the extraction, solvent for Salicaceae (Salicaceae) Plant 1 0.5 to 1000
The weight is preferably 0.5 to 100 times, particularly in terms of extraction operation and efficiency. Further, the extraction temperature is conveniently in the range of room temperature to the boiling point of the solvent or less under normal pressure, and the extraction time is preferably in the range of 2 hours to 2 weeks, although it varies depending on the extraction temperature and the like.

The Salicaceae plant extract thus obtained can be used as it is, but it can be used for deodorizing, decolorizing, concentrating, etc. within the range of not losing the antibacterial effect of the present invention. It may be used as a fractionated product by adding a purification operation or further using column chromatography or the like. These extracts, purified products, and fractions can be made into a dry solid by removing the solvent from them, and can be provided in the form of being solubilized in a solvent such as alcohol, or in the form of an emulsion. it can.

In the present invention, the Salixaceae ( Salicaceae)
ae ) Anti-inflammatory agents used in combination with plant extracts include cortisone, hydrocortisone, prednisolone, methylprednisolone, dexamethasone, betamethasone, triamcinolone, triamcinolone acetonide, fluocinolone acetonide, fluocinonide, beclomethasone and their phosphates, propione. Acid, acetate, succinate and other steroidal anti-inflammatory agents, salicylic acid and aspirin,
Salicylic acid derivatives such as salicylamide, etenzamid, methyl salicylate, indoleacetic acid derivatives such as indomethacin and sulindac, pyrazolidinedione derivatives such as phenylbutazone and oxyphenbutazone, anthranilic acid derivatives such as mefenamic acid and flufenamic acid, ibuprofen, Non-steroidal anti-inflammatory agents such as propionic acid derivatives such as ketoprofen and naproxen, phenylacetic acid derivatives such as diclofenac, fenbufen and bufexamac, benzothiazine derivatives such as piroxicam, glycyrrhizic acid and dipotassium glycyrrhizinate, salts of glycyrrhizinate such as monoammonium glycyrrhizinate. And derivatives, glycyrrhetinic acid and stearyl glycyrrhetinic acid, glycyrrhetinyl stearate, 3-succinyloxyg Glycyrrhetinic acid salts and derivatives such as disodium tilretate, guaiazulene, ethyl guaiazulene sulfonate, sodium guaiazulene sulfonate, azulene derivatives such as camazulene, allantoin, aloin, aloe-emodin, shikonin and isobutylshikonin, acetylshikonin, isovalerylshikonin, etc. And ginsenosides such as ginsenoside R _a1 , ginsenoside R _a2 and ginsenoside R _b1 , and ginsenoside derivatives such as 20-glucoginsenoside R _f , paeonol derivatives such as paeoniflorin, paeonol and paeonoside, paeonolide, and the like.

Further, in the present invention, as anti-inflammatory agents, Scutellariae Radix , Glycyrrhiz
ae Radix ), Kujin ( Sophorae Radix ), Psycho ( Bupl
euriRadix), peony (Paeoniae Radix), Cimicifuga (Cimicifugae Rhizoma), gymnastics (Zizyphi Fruct
us ), Chimo ( Anemarrhenae Rhizoma ), Button pie ( Mout
an Cortex ), Ryutan ( Gentianae Scabrae Radi
x ), forsythia ( Forsythiae Fructus ) and the like, or herbal medicines used as anti-inflammatory agents or extracts thereof can also be used.

For the external preparation for skin according to the present invention, one or more selected from these anti-inflammatory agents are used.

As the anti-inflammatory agent in the present invention, one or more selected from glycyrrhizic acid salts and derivatives, glycyrrhetinic acid salts and derivatives, and licorice are included because of their ease of incorporation into external skin preparations. It is preferable to use one or two selected from stearyl glycyrrhetinate and licorice extract, which are preferably used and have the effect of improving rough skin.

In the present invention, Salicacea ( Salicacea)
e ) A plant extract and one or more anti-inflammatory agents are contained in a skin external preparation base. The amount of the skin external preparation to be compounded is preferably about 0.001 to 5.0% by weight for Salicaceae plant extract. About anti-inflammatory agents, it depends on the type,
It is suitable to set it to about 0.0001 to 5.0% by weight.

The external preparation for skin according to the present invention can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders and granules. In addition, skin cosmetics such as lotion, emulsion, cream, beauty essence, pack,
Makeup base lotion, make-up base cream and other base cosmetics, foundation of each dosage form such as milky liquid, oily and solid form, eye color, cheek color and other make-up cosmetics, hand cream, leg cream, neck cream , Can be provided as body cosmetics such as body lotion.

The external preparation for skin according to the present invention includes oily components, surfactants, humectants, pigments, ultraviolet absorbers, antioxidants, in addition to Salixaceae plant extracts and anti-inflammatory agents. It is possible to contain general raw materials for medicines and cosmetics such as fragrances and antibacterial and antifungal agents, and physiologically active ingredients such as skin cell activating agents and whitening agents.

[0020]

EXAMPLES The features of the present invention will be described in detail with reference to Examples.

First, preparation of a Salicaceae plant extract contained in the external preparation for skin according to the present invention will be described.

[Crown willow bark extract] To 200 g of fresh bark of Salix nigra collected in the spring was shredded, 1000 g of purified water was added, and the mixture was extracted at 25 ° C for 36 hours with stirring. The obtained extract was filtered and then concentrated under reduced pressure to obtain 150 g of willow bark extract.

[European porcupine leaf extract] 200 g of fresh leaves of European porcupine ( Populus tremula )
1000g of purified water is added to the shredded product,
Extract for 36 hours with stirring. The obtained extract was filtered and then concentrated under reduced pressure to obtain 120 g of a porcupine leaf extract.

[European willow bark extract] Dried European willow ( Salix alba L .; Salix aurea Salis
b.) 50g is crushed and 1000g of 50% by volume ethanol
And extract at room temperature for 24 hours. The obtained extract was filtered, concentrated under reduced pressure, and then freeze-dried to obtain 5 g of a willow bark extract.

[Weeping willow leaf extract] 10 g of fresh weeping willow ( Salix babylonica L.) sprouts
Purified water (100 g) is added to and the mixture is stirred and extracted with a mixer for 10 minutes. The obtained extract was filtered, concentrated under reduced pressure, and lyophilized to obtain 1.2 g of weeping willow leaf extract.

The anti-inflammatory agents used in the following Examples of the present invention were those commercially available for pharmaceuticals or cosmetics. The preparation of the extract of the anti-inflammatory crude drug is shown below.

[Ougon Extract] Ougon ( Scutellari
ae Radix ) 300g is dried and crushed, and ethanol 0.5
Add to the liter and let stand at 20 ° C for 10 days for extraction,
The filtrate was collected by filtration. The filtrate was concentrated under reduced pressure and freeze-dried to obtain the title extract.

[Licorice extract] licorice ( Glycyrrhiz
500 g of ae Radix ) was dried, pulverized, and extracted in 1 liter of hot water for 2 hours. The filtrate was collected by filtration, concentrated under reduced pressure, and then freeze-dried to obtain the title extract.

[Kuzin Extract] Kuzin ( Sophorae Radi
x ) 500 g was dried, pulverized, immersed in 1 liter of 50% by volume aqueous ethanol, and extracted at 25 ° C. for 7 days. The filtrate was collected by filtration and used as the title extract.

[Peonies extract] Peonies ( Paeoni
ae Radix ) 550 g is dried, pulverized, immersed in 1 liter of 50% by volume ethanol aqueous solution, and stirred for 20
Extraction was carried out at 0 ° C for 10 days. Then collect the filtrate by filtration,
After concentrating under reduced pressure, it was freeze-dried to obtain the title extract.

[ Thailand extract] Thion ( Zizyphi Fr
uctus ) (600 g) was dried, crushed, immersed in 1 liter of 50% by volume ethanol aqueous solution, and extracted at 25 ° C. for 7 days. The filtrate was collected by filtration and used as the title extract.

[ Button pie extract] Button pie ( Moutan Cor
tex ) (520 g) was dried, pulverized, immersed in 1 liter of ethanol, allowed to stand at 10 ° C. for 14 days, and extracted. The filtrate was collected by filtration and used as the title extract.

[Ryutan extract] Ryutan ( Gentia
nae Scabrae Radix ) 650g is dried and crushed, and hot water 1
Extracted in liter for 4 hours. The filtrate was collected by filtration, concentrated under reduced pressure and then freeze-dried to obtain the title extract.

[Forsythia extract] Forsythia ( Forsyt
hiae Fructus ) 750 g is crushed, immersed in 1.2 liters of 1,3-butylene glycol, and stirred at 15 ° C.
It was extracted for 10 days. The filtrate was collected by filtration and used as the title extract.

Next, the formulation of the examples of the external preparation for skin according to the present invention will be shown.

Example 1 Lotion (1) Ethanol 20.0 (wt%) (2) Polyoxyethylene (60 E.O.) hydrogenated castor oil 1.0 (3) Willow bark extract 1.0 ( 4) Dipropylene glycol 5.0 (5) 1,3-butylene glycol 10.0 (6) Sodium guaiazulene sulfonate 0.2 (7) Methyl paraoxybenzoate 0.1 (8) Purified water 62.7 Production method: Add the components (1) to (7) in sequence to (8) and dissolve,
Make uniform

Example 2 Emulsion (1) Cetanol 1.0 (wt%) (2) Beeswax 0.5 (3) Vaseline 2.0 (4) Squalane 6.0 (5) Dimethylpolysiloxane 2.0 (6) Polyoxyethylene (20 E.O.) sorbitan 1.0 monostearate (7) glyceryl monostearate 1.0 (8) glycerin 4.0 (9) 1,3-butylene glycol 4.0 (10) Methyl paraoxybenzoate 0.1 (11) Purified water 62.6 (12) Carboxyvinyl polymer 10.0 (1.0 wt% aqueous solution) (13) Potassium hydroxide (10.0 wt% aqueous solution) 1 0.0 (14) European porcupine leaf extract 0.5 (15) allantoin 0.2 (16) ethanol 5.0 Production method: The oil phase components of (1) to (7) are mixed, heated and dissolved to give 75
℃. On the other hand, the water phase components (8) to (11) are mixed and dissolved to 75 ° C. After pre-emulsifying by adding the oil phase to this, add (12) and homogenize with a homomixer, then add (13) to thicken and cool, and cool at 40 ° C (14). ~
Add (16) and mix.

Example 3 Emulsion (1) Cetanol 1.0 (wt%) (2) Beeswax 0.5 (3) Vaseline 2.0 (4) Squalane 6.0 (5) Dimethylpolysiloxane 2.0 (6) Polyoxyethylene (20E.O.) sorbitan 1.0 Monostearate (7) Glyceryl monostearate 1.0 (8) Stearyl glycyrrhetinate 0.2 (9) Glycerin 4.0 (10) 1,3-Butylene glycol 4.0 (11) Methyl paraoxybenzoate 0.1 (12) Purified water 61.9 (13) Carboxyvinyl polymer 10.0 (1.0% by weight aqueous solution) (14) Potassium hydroxide (10.0 wt% aqueous solution) 1.0 (15) Ethanol 5.0 (16) Scutellaria extract 0.2 (17) Willow bark extract 0.1 Process: Oil phase of (1) to (8) Mix the ingredients Heated and dissolved to 75
℃. On the other hand, the aqueous phase components (9) to (12) are mixed and dissolved to 75 ° C. After adding the oil phase to this and pre-emulsifying, add (13) and homogenize with a homomixer, then add (14) to thicken and cool, and cool at 40 ° C (15). ~
Add (17) and mix.

[Example 4] Oil-in-water cream (1) Beeswax 6.00 (wt%) (2) Cetanol 5.00 (3) Reduced lanolin 8.00 (4) Squalane 27.50 (5) Glycyrrhetin Acid stearyl 0.05 (6) Glyceryl fatty acid ester 4.00 (7) Lipophilic glyceryl monostearate 2.00 (8) Polyoxyethylene (20E.O.) sorbitan 5.00 Monolaurate (9) Stearyl glycyrrhetinate 0.25 (10) Propylene glycol 5.00 (11) Methyl paraoxybenzoate 0.10 (12) Purified water 36.80 (13) Weeping willow leaf extract 0.10 (14) Licorice extract 0.1. 20 Production method: Mix and dissolve the oil phase components (1) to (9) to 75 ° C. On the other hand, the water phase components (10) to (12) are mixed and dissolved, and
Heat to 5 ° C. Next, the oil phase component is added to the aqueous phase component to pre-emulsify it, then homogenize it with a homomixer, and after cooling, (13) and (14) are added and mixed at 40 ° C.

[Example 5] Oil-in-water cream (1) Beeswax 6.0 (wt%) (2) Cetanol 5.0 (3) Reduced lanolin 8.0 (4) Squalane 27.5 (5) Glyceryl Fatty acid ester 4.0 (6) Lipophilic glyceryl monostearate 2.0 (7) Polyoxyethylene (20E.O.) sorbitan 5.0 Monolaurate (8) Propylene glycol 5.0 (9) Paraoxy Methyl benzoate 0.1 (10) Purified water 37.0 (11) Willow bark extract 0.3 (12) Kujin extract 0.1 Manufacturing method: Mix and dissolve the oil phase components of (1) to (7) To 75 ° C. On the other hand, the water phase components (8) to (10) are mixed and dissolved, and
Heat to 5 ° C. Next, the oil phase component is added to the aqueous phase component to pre-emulsify it, then homogenize with a homomixer, and after cooling, (11) and (12) are added and mixed at 40 ° C.

[Example 6] Gel agent (1) Dipropylene glycol 10.0 (wt%) (2) Carboxyvinyl polymer 0.5 (3) Methyl paraoxybenzoate 0.1 (4) European porcupine leaf extract 0.2 (5) Peony extract 0.3 (6) Potassium hydroxide (10.0 wt% aqueous solution) 1.0 (7) Purified water 87.9 Production method: (7) to (1) to (5) Is uniformly dissolved, and then (6) is added to increase the viscosity.

[Example 7] Oil-in-water emulsion type ointment (1) White petrolatum 25.0 (% by weight) (2) Stearyl alcohol 25.0 (3) Glycerin 12.0 (4) Sodium lauryl sulfate 1.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 35.8 (7) Willow bark extract 0.1 (8) Button pipi extract 1.0 Process: Oil phase of (1) to (4) The ingredients are mixed and heated to dissolve uniformly, and the temperature is adjusted to 75 ° C. On the other hand, the water phase components (5) to (6) are mixed and heated to 75 ° C. After gradually adding the oil phase component to the water phase component with stirring to emulsify and cool,
Add (7) and (8) at 40 ° C. and mix.

[Example 8] Liposome agent (1) Glycerin 2.0 (% by weight) (2) 1,3-butylene glycol 3.0 (3) Polyoxyethylene (25E.O.) oleyl ether 0.2 (4) Ethanol 10.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 79.7 (7) Black willow bark extract, licorice 5.0 Extracted liposome-encapsulating method: (5) to (4) Dissolve in, add to (6) together with (1) to (3), mix uniformly, and add (7) to this and disperse.
In addition, (7) the willow bark extract and licorice extract-encapsulating liposomes are as follows: 1.0 (w / v)% of the willow bark extract
And 80 g of soybean lecithin in 100 mL of 50% by volume aqueous ethanol solution containing 2.0 (w / v)% of licorice extract.
Was added and suspended at 55 ° C., and then sonicated to prepare liposomes, which were recovered by centrifugation.

[Example 9] Water-in-oil type emollient cream (1) Liquid paraffin 30.0 (wt%) (2) Microcrystalline wax 2.0 (3) Vaseline 5.0 (4) Diglyceryl dioleic acid Ester 5.0 (5) L-Glutamate sodium 1.6 (6) L-serine 0.4 (7) Propylene glycol 3.0 (8) Methyl paraoxybenzoate 0.1 (9) Weeping willow leaf extract 0. 1 (10) Forsythia extract 1.5 (11) Purified water 51.2 (12) Perfume 0.1 Manufacturing method: (5) and (6) are dissolved in a part of (11) to 50 ° C.,
Gradually add to (4) previously heated to 50 ° C. with stirring. This is mixed in advance and uniformly dispersed in (1) to (3) which is heated and dissolved at 70 ° C. To this, (7)-(10)
Is added to the rest of (11), heated to 70 ° C., added with stirring, and emulsified with a homomixer. 40 after cooling
Add (12) at ℃ and mix.

[Example 10] Make-up base cream (1) Stearic acid 12.00 (% by weight) (2) Cetanol 2.00 (3) Glyceryl tri-2-ethylhexanoate 2.50 (4) Self-emulsification Type Glyceryl monostearic acid 2.00 Ester (5) Propylene glycol 10.00 (6) European porcupine leaf extract 0.02 (7) Methyl paraoxybenzoate 0.10 (8) Ryutan extract 0.05 (9) Potassium hydroxide 0.30 (10) Purified water 68.43 (11) Titanium oxide 2.00 (12) Red iron oxide 0.40 (13) Yellow iron oxide 0.10 (14) Perfume 0.10 Manufacturing method: (1) The oil phase components (4) to (4) are mixed and dissolved to 75 ° C. On the other hand, the water phase components (5) to (10) are mixed, heated and dissolved, and the pigment components (11) to (13) are added thereto, and the mixture is uniformly dispersed with a homomixer to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer. After cooling, (14) is added and mixed at 40 ° C.

Example 11 Milky liquid foundation (1) Stearic acid 2.00 (% by weight) (2) Squalane 5.00 (3) Octyldodecyl myristate 5.00 (4) Cetanol 1.00 (5) Decaglyceryl monoisopalmitic acid ester 9.00 (6) 1,3-butyleneglycol 6.00 (7) Methyl paraoxybenzoate 0.10 (8) Potassium hydroxide 0.08 (9) Purified water 52.47 (10) Titanium oxide 9.00 (11) Talc 7.40 (12) Red iron oxide 0.50 (13) Yellow iron oxide 1.10 (14) Black iron oxide 0.10 (15) European willow bark extract 0. 10 (16) Triticum arvense extract 1.00 (17) Perfume 0.15 Manufacturing method: The oil phase components (1) to (5) are mixed and dissolved to 75 ° C. On the other hand, the water phase components (6) to (9) are mixed, heated and dissolved, and the pigment components (10) to (14) are added thereto, and the mixture is uniformly dispersed by a homomixer to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer. After cooling, (15) to (17) are added and mixed at 40 ° C.

Example 12 Hand Cream (1) Cetanol 4.00 (wt%) (2) Vaseline 2.00 (3) Liquid Paraffin 10.00 (4) Glyceryl Monostearate 1.50 (5) Polyoxyethylene (60 E.O.) glyceryl 2.50 Isostearate (6) Tocopherol acetate 0.25 (7) Glycerin 20.00 (8) Methyl paraoxybenzoate 0.10 (9) Weeping willow leaf extract 0.1. 10 (10) Dipotassium glycyrrhizinate 0.02 (11) Purified water 59.53 Production method: The oil phase components (1) to (6) are mixed and dissolved to 75 ° C. On the other hand, the water phase components (7) to (11) are mixed and dissolved, and
Set to 5 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer and cooled.

Example 13 Pack (1) Purified Water 69.0 (wt%) (2) Polyvinyl Alcohol 12.5 (3) Ethanol 10.0 (4) Glycerin 5.0 (5) Polyethylene Glycol (Average Molecular weight 1540) 3.0 (6) Willow bark extract 0.3 (7) Licorice extract 0.2 Production method: Components (2) to (7) are sequentially added to (1),
Mix, dissolve and homogenize.

[0049]   [Example 14] Heart nick (1) Ethanol 50.0 (wt%) (2) Purified water 48.4 (3) Dipotassium glycyrrhizinate 0.1 (4) European porcupine leaf extract 1.5 Manufacturing method: Components (1) to (4) are mixed and uniformly dissolved.

[Example 15] Hair shampoo (1) Sodium alkyl ether sulfate 18.0 (% by weight) (2) Coconut oil fatty acid diethanolamide 2.0 (3) Propylene glycol 2.0 (4) European willow bark extraction Product 0.1 (5) Allantoin 0.1 (6) Purified water 77.8 Manufacturing method: The components (1) to (5) are added to (6) in sequence,
Make it uniform.

Example 16 Hair rinse (1) Cetanol 3.0 (wt%) (2) Stearyl trimethyl ammonium chloride 0.7 (3) Glycerin 3.0 (4) N-cocoyl-L-arginine ethyl ester -DL-pyrrolidone carboxylate 0.1 (5) Allantoin 0.1 (6) Weeping willow leaf extract 0.3 (6) Purified water 92.8 Production method: The components of (1) to (5) are sequentially (6 ),
Mix.

[Example 17] Liquid body shampoo (1) N-lauroyl-L-glutamic acid 20.0 (% by weight) Triethanolamine (30.0% by weight aqueous solution) (2) N-lauroylmethyl taurine sodium 10. 0 (30.0 wt% aqueous solution) (3) triethanolamine laurate 10.0 (4) triethanolamine myristate 10.0 (5) lauroyl imidazolinium betaine 5.0 (6) lauroyl diethanolamide 5. 0 (7) Propylene glycol 7.0 (8) Black willow bark extract 1.0 (9) Triturium extract 0.5 (10) Purified water 41.5 Process: The components of (1) to (9) are sequentially added. Add to (10) and mix.

Example 18 Face Wash Foam (1) Myristic Acid 18.0 (wt%) (2) Palmitic Acid 3.0 (3) Stearic Acid 7.0 (4) Mixed Fatty Acid Triglyceride 0.1 (5) Stearyl glycyrrhetinate 0.1 (6) Self-emulsifying glyceryl monostearate 3.0 (7) Purified water 38.2 (8) Glycerin 17.0 (9) Potassium hydroxide 7.8 (10) Diglycerin 3. 0 (11) 1,3-butylene glycol 1.0 (12) N-stearolyl-L-glutamate disodium 1.0 (13) Methyl paraoxybenzoate 0.1 (14) Weeping willow leaf extract 0.1 (15 ) Stearyl glycyrrhetinate 0.1 (16) Licorice extract 0.5 Production method: The oil phase components (1) to (6) are mixed and heated to 70 ° C. The aqueous phase components (7) to (16) are mixed, dissolved and heated to 70 ° C. The above oil phase is gradually added to this aqueous phase component for saponification, and then cooled while mixing.

Among the examples according to the present invention described above, Examples 1 to 8 were evaluated for their inhibitory effect on the formation of wrinkles on the skin caused by medium wavelength ultraviolet rays (UVB). At that time, in Examples 1 to 8, the Salicaceae plant extract and the anti-inflammatory agent, or the black willow bark extract and the licorice extract-encapsulating liposome, respectively, which were blended in Examples 1 to 8,
As shown in Table 1, instead of Comparative Examples 1 to 8, Comparative Examples 1 to 8 were evaluated at the same time. The evaluation was carried out with 5 hairless mice as one group, and each group was 0.2% in Examples and Comparative Examples.
G was applied once a day to the back and irradiated with 100 mJ / cm ² / time of UVB three times a week for 20 weeks, and the appearance of wrinkles on the skin of hairless mice was observed, according to the criteria shown in Table 2. Evaluation was made by scoring. At this time, a group to which only purified water was applied served as a control. The results are shown in Table 3 by calculating the average value of each group and the relationship with the number of UVB irradiation days.

[0055]

[Table 1]

[0056]

[Table 2]

[0057]

[Table 3]

As is clear from Table 3, in the control, the wrinkles formed on the skin reached a moderate depth when the UVB irradiation days exceeded 10 weeks, and deep wrinkles were formed after 20 weeks. Was recognized. Comparative Example 1 containing only an extract of Salicaceae plant as an active ingredient
In Comparative Example 6 application group, slight wrinkle formation was observed after 20 weeks, wrinkle formation was well suppressed, and an anti-inflammatory agent was contained, and Salixaceae plant extract was added. In other comparative application groups that did not contain it, an inhibitory effect on wrinkle formation was recognized as compared with the control, but in each of the corresponding Example application groups, formation of minute wrinkles was observed after 20 weeks. Thus, the inhibitory effect on wrinkle formation was significantly improved as compared to the corresponding comparative application group.

Subsequently, use tests were conducted on Examples 1 to 13 of the present invention to evaluate the effect of improving skin aging symptoms. At that time, the blended Salixaceae plant extract and anti-inflammatory agent in Examples 9 to 13 were replaced as shown in Table 4 to give Comparative Examples 9 to 13.
It was subjected to a usage test together with Comparative Examples 1 to 8 above.

[0060]

[Table 4]

As for the effect of improving the skin aging condition, fine wrinkles and a decrease in skin elasticity are noticeable.
20 female panelists in their teens were set as one group, and each of the examples and the comparative examples was blindly used twice a day for 2 months continuously and evaluated. The degree of fine wrinkles is evaluated by visual observation and photography, and the skin elasticity is measured by a cute meter, and the states before and after the use test are compared, respectively, and “improved”, “slightly improved”, and “changed”. The evaluation was made in three grades of "none". The results are shown in Table 5 by the number of panelists who obtained each evaluation.

[0062]

[Table 5]

As is clear from Table 5, Comparative Examples 2 to 5, Comparative Example 5, Comparative Example 7, Comparative Example 8 and Comparative Example containing an anti-inflammatory agent as an active ingredient but not a Salicaceae plant extract. In each use group of 10 to Comparative Example 12,
Although there was a tendency to improve fine wrinkles and skin elasticity, not many panelists recognized a clear improvement. In each of the use groups of Comparative Example 1, Comparative Example 6, Comparative Example 9, and Comparative Example 13 containing only the Salixaceae plant extract, fairly good fine wrinkles and a tendency of improving skin elasticity were observed. However, in the group used in Examples,
Compared to the corresponding groups using the comparative examples, the number of panelists with a clear improvement was significantly greater.

Further, with respect to Examples 1 to 13 and Comparative Examples 1 to 13 of the present invention, the effect of improving rough skin symptoms was evaluated. The effect of improving skin roughening symptoms was as follows. Twenty female panelists in their twenties to sixties exhibiting remarkable skin roughening symptoms were treated as one group, and each of the Examples and Comparative Examples was blinded twice a day to each group. It was evaluated by allowing it to be used continuously for a month. The skin condition before and after the use test was evaluated and scored according to the evaluation criteria shown in Table 6, and the average value of 20 persons was calculated and shown in Table 7.

[0065]

[Table 6]

[0066]

[Table 7]

As is clear from Table 7, in any of the groups used in the examples of the present invention, remarkable improvement in rough skin was observed, and after the use test was completed, the skin condition was improved to almost good to good condition. It had been. On the other hand, in the comparative example use group, a considerably good improvement of the rough skin was recognized, but the degree thereof was smaller than that of the corresponding example use group.

Regarding Example 1 to Example 13, 2
No change in condition was observed when stored at 5 ° C for 6 months, and no problem skin irritation reaction was observed in a 48-hour back occlusion patch test conducted by 30 male panelists.

[0069]

Industrial Applicability As described in detail above, according to the present invention, it is possible to obtain a skin external preparation which is excellent in stability and safety, and has synergistically improved effects of preventing and improving rough skin and skin aging. .

   ─────────────────────────────────────────────────── ─── Continued front page    F-term (reference) 4C083 AA082 AA11 AB032 AB232                       AB242 AB432 AC012 AC022                       AC072 AC102 AC122 AC182                       AC242 AC392 AC432 AC442                       AC482 AC542 AC582 AC692                       AC712 AC792 AD092 AD112                       AD152 AD512 AD532 AD662                       BB51 CC02 CC03 CC05 CC12                       CC23 CC37 CC38 CC39 DD08                       DD22 DD27 DD31 DD33 DD41                       DD45 EE12 EE23                 4C084 AA19 DA01 MA02 NA05 ZA891                       ZB112                 4C088 AB12 AC01 AC06 CA05 MA02                       MA63 NA14 ZA89 ZB11

Claims (4)

[Claims] 1. A skin external preparation comprising a Salicaceae plant extract and one or more anti-inflammatory agents. Wherein Salicaceae (Salicaceae) extracts of plants is a water extract of Salicaceae (Salicaceae) plants, skin external preparation according to claim 1. 3. Salicaceae (Salicaceae) extracts of plants is an extract from the bark of Salicaceae (Salicaceae) plants, skin external preparation according to claim 1 or claim 2. 4. The external preparation for skin according to claim 1, wherein the extract of Salicaceae plant is Salix nigra L. extract.