JP2003321673A - Production system for antifreezing agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To carry out an effective utilization of wastes by producing an antifreezing agent from waste of shells and waste of vegetables. <P>SOLUTION: The system produces calcium acetate as the antifreezing agent, comprising a calcium production system 1 which produces calcium carbonate and or calcium oxide from shells of scallop, an acetic acid production system 10 provided with an ethanol producing section 11 producing ethanol using apple marc which is a waste of vegetables as raw materials and an acetic acid producing section 20 which produces acetic acid from the ethanol, a calcium acetate producing apparatus 40 producing calcium acetate from calcium carbonate produced in the calcium production system 1 and or calcium oxide and acetic acid produced in the acetic acid production system 10 by mixing and warming, and a fodder producing apparatus 50 producing the fodder from the residue produced in production of acetic acid in the acetic acid production system 10. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a system for producing an antifreezing agent for melting snow and preventing freezing of snow and ice in cold regions and the like, and particularly to producing calcium acetate as an antifreezing agent. The present invention relates to an antifreezing agent manufacturing system.

[0002]

2. Description of the Related Art Conventionally, various substances have been used as antifreezing agents. Among them, chlorides are associated with the problem of salt damage. Therefore, acetate freezing agents are used instead. Is starting to appear. Examples of acetate-based antifreezing agents include potassium acetate, sodium acetate,
Calcium acetate and the like are known.

[0003]

By the way, in recent years, much research has been conducted on the effective use of waste, particularly in Aomori prefecture, which produces a large amount of apples, which are wastes of fruits and vegetables. Studies have also been conducted on pressed meal and scallop shells. Also, since Aomori Prefecture has a large amount of snowfall and is cold, the actual amount of the above-mentioned antifreezing agent used is very large. Against this background, the inventors of the present application have been studying the use of pressed meal of apples and shells of scallops as antifreezing agents. However, even if one tries to simply use the squeezed lees of apples and the shells of scallops for antifreeze technology,
It's not easy.

The present invention has been made in view of the above problems, and it is possible to produce an antifreezing agent by using the waste of shells and the waste of fruits and vegetables as a raw material, and the freezing for effective utilization of the waste. It is an object of the present invention to provide an inhibitor manufacturing system.

[0005]

Means for Solving the Problems The technical means of the present invention for solving the above-mentioned problems is to use calcium carbonate as a raw material for calcium carbonate in a production system of an antifreezing agent for producing calcium acetate as an antifreezing agent. And / or a calcium production apparatus for producing calcium oxide, and an acetic acid production apparatus for producing acetic acid from waste of fruits and vegetables,
Calcium carbonate manufactured by a calcium manufacturing device and /
Alternatively, the apparatus is provided with a calcium acetate production apparatus for producing calcium acetate by mixing calcium oxide and acetic acid produced by the acetic acid production apparatus.

Thus, when producing the antifreezing agent, calcium carbonate and / or calcium oxide is produced from the shell as a raw material in the calcium producing apparatus.
On the other hand, in an acetic acid production apparatus, acetic acid is produced using fruits and vegetables waste as a raw material. Then, in the calcium acetate production apparatus, calcium carbonate and / or calcium oxide produced by the calcium production apparatus and acetic acid produced by the acetic acid production apparatus are mixed to produce calcium acetate. Therefore, it becomes possible to manufacture an antifreezing agent using the waste of shells and the waste of fruits and vegetables as raw materials, and the waste can be effectively used.

Then, if necessary, the above calcium producing apparatus comprises a washing machine for washing the shell and a crushing machine for crushing the washed shell to obtain calcium carbonate particles. . This makes it possible to easily supply calcium carbonate. Further, if necessary, the calcium producing apparatus is configured to include a calcining machine that calcinates the calcium carbonate powder and granules to form calcium oxide powder and granules. This makes it possible to easily supply calcium oxide.

Further, if necessary, the acetic acid production apparatus is equipped with an ethanol production section for producing ethanol from waste of fruits and vegetables and an acetic acid production section for producing acetic acid from ethanol produced in the ethanol production section. I am configuring. Since the waste of fruits and vegetables is fermented with ethanol and then with acetic acid, the steps are separated, and the fermentation efficiency is extremely improved.

In this case, the ethanol producing section is used as a raw material for Candida Shehae.
It is effective to have an ethanol fermenter for inoculating the mutant strain tae) and fermenting the raw material while stirring at a predetermined temperature. In order to produce ethanol from fruits and vegetables such as squeezed lees of apples, the inventors of the present application have conducted research on a microorganism having a property of high ethanol production, and as a microorganism, Candida Shhatae (Candida Sh
ehatae). In particular, as microorganisms, the deposit number "FERM P-1" was obtained at the Patent Biological Depositary Center of the Institute of Biotechnology, Institute of Advanced Industrial Science and Technology, Ministry of Economy, Trade and Industry.
8829 ”deposited as Candida Shahatae (C
andida Shehatae) mutant strain (variant strain)
Candida She
hata) HYO-503 strain is effective.

More specifically, as a microorganism, Candida Shehatae HYO-
503 strain was developed, and in particular, a method was invented for treating fruits and vegetables having a relatively small fermentation source such as glucose such as squeezed lees of apples so as to be effective. In order to produce acetic acid from fermented apple meal by fermentation, it is very important to improve the efficiency of the ethanol fermentation process. Here, Candida S
the heatae IAM 12953 strain as a parent strain,
Mutagenizing treatment was performed with ultraviolet rays (UV) to obtain a large number of strains that grow on a potato dextrose agar medium containing 5.0% ethanol. Among them, the mutant strain HYO-50 with the highest ethanol conversion rate in potato dextrose agar medium
3 strains were obtained. The HYO-503 strain was inoculated into 10 mL of 5.0% ethanol potato dextrose agar medium at a ratio of 1%, and statically cultured at 30 ° C. for 5 days.

This procedure was repeated, and after acclimation in a liquid medium containing 5.0% ethanol, 5.25% ethanol was obtained in order to obtain a strain that withstands higher concentration of ethanol and grows early. The culture was repeated for 7 days in a liquid medium containing acclimation, and the culture was acclimated to 5.2.
A mutant strain HYO-503, which is resistant to 5% ethanol and has an efficient ethanol-producing ability, was obtained from apple dregs. The obtained mutant strain retains 5.25% ethanol resistance well even after being subcultured several times in a 5.25% ethanol-free liquid medium, and retains the ability to produce ethanol from apple dregs. Was done. By the above operation, the 5.25% ethanol-resistant strain HYO-503 strain of the present invention was obtained.

As a result of examining the bacteriological properties of the mutant strain HYO-503 (see Experimental Examples described later), the mutant strain HYO-50
3 strains are Candida Shehatae IAM 1
Since this strain showed the same properties as 2953, this strain was Ca
Like Ndida Shehatae IAM 12953, Candida Shehatae
hatae).

Also, Candida Shahatae (Candi
The Da Shehatae) HYO-503 strain is superior to the parent strain in that it has high ethanol resistance. The ethanol tolerance is an important property as described below. It ’s Candida S.
hehatae) is a bacterium that produces ethanol,
When you produce a certain amount of ethanol, you die with the ethanol that you produced. These properties are not unique to Candida Shehatae, but also apply to bacteria that make general sake, and to other properties (not only ethanol but also organic acids, antibiotics, etc.) Most of the products produced in (1) die when the amount exceeds a certain amount, so production is stopped in most cases. Therefore, in order to improve the yield (productivity) of ethanol, a strain having a (resistant) property that enables growth even if the ethanol concentration is high is required. In this respect, Candida Shahatae
The Shehatae) HYO-503 strain is highly resistant to ethanol and is excellent.

In the present invention, if necessary, the above-mentioned acetic acid-producing section is inoculated into a raw material with acetic acid bacteria and fermented with stirring at a predetermined temperature. It comprises a centrifuge for centrifuging the finished raw material to extract acetic acid, and an acetic acid storage tank for storing the acetic acid separated by the centrifuge. Acetic acid with high purity and high concentration can be supplied to the calcium acetate production apparatus from the acetic acid storage tank, and the production efficiency can be improved.

Further, the above calcium acetate producing apparatus is provided with a mixing mixer for spraying acetic acid on the supplied calcium carbonate and / or calcium oxide and stirring while heating. Mixing is performed smoothly, and the production efficiency of calcium acetate can be improved. Furthermore,
If necessary, the above-mentioned acetic acid production apparatus is provided with a feed production apparatus for producing feed from the residue produced when acetic acid is produced. Since the residue is also used, waste can be effectively used without waste.

If necessary, squeezed lees of apple are used as a waste of fruits and vegetables. Furthermore, if necessary,
The scallop shell is used as the shell. It is possible to make effective use of these wastes.

[0017]

BEST MODE FOR CARRYING OUT THE INVENTION A manufacturing system for an antifreezing agent according to an embodiment of the present invention will be described below with reference to the accompanying drawings. As shown in FIG. 1, the antifreezing agent production system according to the embodiment is a system for producing calcium acetate as an antifreezing agent, and its basic configuration is to use calcium carbonate and / or oxidized calcium from a shell as a raw material. A calcium producing apparatus 1 for producing calcium, an acetic acid producing apparatus 10 for producing acetic acid from waste of fruits and vegetables, a calcium carbonate and / or calcium oxide produced by the calcium producing apparatus 1, and an acetic acid producing apparatus 10. And a calcium acetate producing apparatus 40 for producing calcium acetate by mixing with acetic acid.

This production system uses squeezed lees of apple as a waste of fruits and vegetables. Apple cake is a juice residue produced from commercially available apple juice and can be obtained from an apple juice manufacturing plant or the like. It should be noted that the present invention is not limited to squeezed lees of apples. In addition, scallop shells as waste are used as shells. It is a shell obtained by collecting scallops from natural or cultured scallops and is obtained from scallop processing plants and the like. The present invention is not limited to scallops.

As shown in FIGS. 1 and 2, the calcium producing apparatus 1 includes a washing machine 3 for washing the shells carried by the carrying machine 2 and charged with tap water, and a washing machine 3 through a conveyor 4. A crusher 5 that grinds the washed and fed shells into calcium carbonate powder and granules; and a calcium carbonate powder and granules that are transferred from the grinder 5 via a conveyor 6 And a calcining device 7 for converting the powder into particles of calcium oxide. In FIG. 2, reference numeral 8
Reference numerals a and 8b are cyclones and exhaust fans that process the gas from the calciner 7, and 9 is a transporter that transports the calcium oxide powder particles to the calcium acetate manufacturing apparatus 40.

The acetic acid production apparatus 10 is shown in FIG. 1, FIG. 3 and FIG.
As shown in FIG. 3, it is configured to include an ethanol production unit 11 that produces ethanol from squeezed lees of apples as a waste of fruits and vegetables, and an acetic acid production unit 20 that produces acetic acid from the ethanol produced by the ethanol production unit 11. ing.

[0021] The ethanol producing unit 11 uses as raw materials enzymes, water, and Candida Sheh.
aata) mutant strain is inoculated and fermented at a predetermined temperature with stirring to ferment the raw material. In FIG. 3, reference numeral 13 is a hopper for supplying the pressed meal of apples, 14 is a stirrer for stirring the raw material of the ethanol fermentation tank 12, and 15 is heating and cooling the ethanol fermentation tank 12 with steam and cooling water to adjust the temperature to a predetermined temperature. A temperature controller, 16 is a pump that feeds the fermented raw material to the acetic acid production unit 20.

In the ethanol production section 11, as will be described later, for example, a raw material treatment including (1) pretreatment step and (2) ethanol fermentation step is performed. Pretreatment process (1)
Consists of (1-1) heat treatment step, (1-2) enzyme treatment step, and (1-3) pH adjustment step.

As shown in FIG. 1 and FIG. 3, the acetic acid production unit 20 inoculates the raw material with an acetic acid bacterium, stirs at a predetermined temperature, stirs at a predetermined temperature, and ferments the raw material while fermenting the raw material while aerating.
Is equipped with. In FIG. 3, reference numeral 22 is an air supply device for supplying aseptic air to the acetic acid fermentation tank 21, and 23 is an acetic acid fermentation tank 2.
1 is a stirrer for stirring the raw material, 24 is a temperature controller for controlling the temperature in the acetic acid fermentation tank 21 with cooling water, 25 is a gas vent machine for exhausting unnecessary gas, 26 is the centrifugal separation of the fermented raw material described later. It is a pump that feeds the machine 30. In this acetic acid fermenter 21, for example, acetic acid bacteria for performing acetic acid fermentation are inoculated with the inoculum volume adjusted to 1 × 10 ⁶ / mL, aerated constantly, and the temperature is 25 ° C. Fermented for 14 days.

Further, as shown in FIGS. 1 and 4, the acetic acid production section 20 includes a centrifuge 30 for centrifuging a raw material that has been fermented in an acetic acid fermentation tank 21 to extract acetic acid, and a centrifuge 30. And an acetic acid storage tank 31 that stores the acetic acid separated in. In the acetic acid storage tank 31, glacial acetic acid is appropriately added to adjust the acetic acid concentration to, for example, about 40%. Figure 4
In the figure, reference numeral 32 is a stirrer for stirring the raw material of the acetic acid storage tank 31, 33 is a temperature controller for controlling the temperature in the acetic acid storage tank 31 with cooling water, and 34 is a calcium acetate production apparatus 40 for acetic acid.
And 35 is a regulator for adjusting the amount of acetic acid supplied by bypassing the acetic acid from the pump 40 to the acetic acid storage tank 31.

As shown in FIGS. 1 and 5, the calcium acetate producing apparatus 40 is supplied with calcium carbonate and / or calcium oxide (calcium oxide in the embodiment).
Mixing mixer 4 in which acetic acid is sprayed on and stirred while heating
1 is provided. In FIG. 5, reference numeral 42 is a hopper that supplies calcium oxide into the mixing mixer 41, 43 is a temperature controller that adjusts the temperature inside the mixing mixer 41 with steam, and 44 and 45 are cyclones that process the gas from the mixing mixer 41. And an exhaust fan.

Further, in the antifreezing agent manufacturing system according to the present embodiment, as shown in FIGS. 1 and 6, the feed manufacturing apparatus for manufacturing feed from the residue produced when acetic acid is manufactured by the acetic acid manufacturing apparatus 10. 50 are provided. As shown in FIG. 6, the feed producing apparatus 50 is configured to include a dryer 51 that dries the residue remaining after the acetic acid has been separated by the centrifuge 30. In FIG. 6, reference numeral 52 denotes a conveyer that conveys the residue and puts it into the dryer 51, 53 a burner device that supplies a heat source to the dryer 51, and 54 discharges and conveys the dried residue from the dryer 51 as feed. Conveyors 55 and 56 are cyclones and exhaust fans that process gas from the feed on conveyor 54.

Therefore, according to the system for producing an antifreezing agent according to this embodiment, the antifreezing agent is produced as follows. First, in the calcium producing apparatus 1, as shown in FIGS. 1 and 2, when a carrier 2 conveys a shell and puts it into the washing machine 3, the washing machine 3 washes the put-in shell with tap water. It After that, the washed shell is conveyed to the crusher 5 via the conveyor 4 and put into the crusher 5, and the shell washed by the crusher 5 is crushed to form calcium carbonate particles. Then, the powdery particles of calcium carbonate are conveyed from the crusher 3 via the conveyor 4 and are put into the baking machine 7 to be baked. As a result, calcium oxide particles are formed. The powdery particles of calcium oxide are carried by the carrier 9 to the hopper 42 of the calcium acetate manufacturing apparatus 40.

On the other hand, in the acetic acid production apparatus 10, FIG.
As shown in FIG. 3, the ethanol production unit 11 processes the raw material including, for example, (1) pretreatment step and (2) ethanol fermentation step. The pretreatment step (1) is (1-
1) heat treatment step, (1-2) enzyme treatment step, (1-
3) Consists of a pH adjusting step. Each step will be described below.

(1) Pretreatment Step (1-1) Heat Treatment Step An appropriate amount (for example, twice the amount) of water is added to the squeezed lees of apples to make them fluid and boiled, for example, at 100 ° C. for 30 minutes.

(1-2) Enzyme treatment step Enzyme treatment is performed by adding an enzyme that increases glucose.
As the enzyme, a commercially available appropriate one such as fibrinolytic enzyme is used. For example, using a stirrer at a speed of 200 rpm, 4
The treatment is performed at 0 ° C. for 24 hours, and the amount of glucose produced by the enzyme treatment is set to 2820 mg / L, for example. After that, for inactivating the enzyme, for example, a treatment at 120 ° C. for 10 minutes may be performed.

(1-3) pH adjusting step For example, sodium bicarbonate is used to adjust the pH to 5.0-8.
It is set to 0, preferably 7.0 ± 0.5.

(2) Ethanol Fermentation Step In this step, as a microorganism, as described above, the deposit number "FERM P-188" was deposited at the Patent Biological Deposit Center of the Institute of Biotechnology, National Institute of Advanced Industrial Science and Technology, Ministry of Economy, Trade and Industry.
29 ”deposited by Candida Shahatae (Can
Candida Shehatae H which is a mutant strain of dida Shehatae)
The YO-503 strain is used. Candida Shahatae (Ca
Ndida Shehatae) HYO-503 strain is inoculated with, for example, 1 × 10 ⁵ / mL, and the culture temperature is 20 ° C. to 37 ° C., for example, 30 ° C. for 13 days, depending on the case. Conducts anaerobic culture while blowing carbon dioxide.

When the ethanol fermentation is completed, the raw material is fed to the acetic acid fermentation tank 21 by the pump 16 as shown in FIGS. In this acetic acid fermentation tank 21, for example, acetic acid bacteria for performing acetic acid fermentation are inoculated with the inoculated amount adjusted to 1 × 10 ⁶ / mL,
Aerate constantly, the temperature is 25 ℃ for 14 days,
Fermentation process. Then, once the fermentation process is over,
As shown in FIGS. 1 and 4, the raw material is conveyed to the centrifuge 30 by the pump 26. As a result, the acetic acid is extracted by centrifugation in the centrifuge 30. The extracted acetic acid is stored in the acetic acid storage tank 31.

Then, as shown in FIGS. 1 and 5, the treatment is carried out by the calcium acetate producing apparatus 40. In this case, calcium oxide is fed from the hopper 42 into the mixing mixer 41, while acetic acid is supplied from the acetic acid storage tank 31 by the pump 34. In the mixing mixer 41, acetic acid is sprayed and calcium oxide and acetic acid are stirred while being heated, thereby reacting with each other to generate calcium acetate. When the required amount of calcium acetate is produced, it is discharged from the mixing mixer 41.

Further, in the feed producing apparatus 50, FIG.
Further, as shown in FIG. 6, the residue remaining after the acetic acid has been separated by the centrifugal separator 30 is conveyed to the dryer 51 and dried. This drying produces a residue as feed.

In the above embodiment, the calcium producing apparatus 1 is configured to produce calcium oxide. However, the present invention is not limited to this, and shells are pulverized by the pulverizer 5 to produce calcium carbonate. Only the structure of forming the powder or granules may be used, and the structure may be appropriately changed. In this case, as shown in line (A) of FIG. 1, the shells are crushed by the crusher 5 to form calcium carbonate powder and granules, which are conveyed to the calcium acetate production apparatus 40. In the calcium acetate manufacturing apparatus 40, calcium carbonate reacts with acetic acid in the mixing mixer 41 to generate calcium acetate.

[0037]

[Experimental example] Next, Candida Shehatae HY used in the acetic acid production apparatus 10
The example of an experiment about O-503 strain is shown. In addition, an experimental example of production of acetic acid is also shown.

(Experimental Example 1) Candida shahatae (Ca
ndida Shehatae) HYO-503 strain (hereinafter referred to as "mutant strain") and Candida shahatae (Can)
The bacteriological properties of the parent strain of Dida Shehatae (hereinafter referred to as "parent strain") were examined. The morphology of the mutant strain was observed by culturing in potato dextrose agar medium (DIFCO) at 25 ° C. for 3 days. The mutant strain forms circular, white colonies of 2.0 to 2.7 mm, and exhibits irregular rod-shaped or rod-shaped fungal forms. The size of the bacterium is 1.5 to 3.5 × 3.0 to 6.
It is 5 μm. The size of the parental colony is 2.5-3.
At 0 mm, the mutant colony is slightly smaller, but other properties are Candida Shehatae IAM 129.
It was the same as 53. In the culture in potato dextrose liquid medium, the bacterial cells became cloudy and did not form pellicle or ring.

(Experimental Example 2) In addition, in order to clarify the physiological properties of the strain, Craiger Fanley (N.
J. W. Kreger-van Rij) edited by The East Taxonomy Study 3rd Edition (The)
Yeasts a taxonomy study,
3rd Revised and Enlarged
The assimilation of the carbon sources of the mutant strain and the parent strain was examined according to the description of the edition). The results are shown in Fig. 7 (Table 1).

From the above-mentioned fungal morphology, colony morphology, fungal growth, and the results shown in Table 1, the strains of the present invention except for the size of the colonies were Candida Shehatae IA.
Since this strain showed the same properties as M12953, this strain was identified as Candida Shehatae IAM 129.
Same as 53, Candida Sahatae (Candida S
hehatae).

(Experimental Example 3) In order to examine that the mutant strain is superior to the parent strain, the parent strain IAM 12953 strain and the mutant strain HYO-503 strain were cultured in 4% glucose-added potato dextrose liquid medium, respectively. A comparative test was carried out by measuring the ethanol production ability, the bacterial growth ability and the glucose consumption. 5 mL of 200 mL liquid medium
Place in a 00 mL Erlenmeyer flask and add 1 × of the test strain.
The cells were inoculated to give 10 ⁵ cells / mL. This 3
Static culture was carried out at 0 ° C. and changes over time were examined. The ethanol concentration was measured by an enzymatic method (F-kit ethanol, Roche Diagnostics), the culture for measuring the bacterial amount was carried out using a potato dextrose agar medium, and the glucose was measured by an enzymatic method (F-kit glucose / Fructose, Roche Diagnostics). The results are shown in Fig. 8 (Table 2).

From Table 2, the mutant strain reached the stationary phase in which the bacterial growth was 48 hours earlier than the parent strain, and the amount of ethanol produced was 5 days earlier than the parent strain, showing a maximum value of 17.60 g / L. . This can explain that the mutant strain has excellent ethanol-producing ability in terms of glucose consumption. In general, the advantage of the mutant strain can be clearly explained by the fact that 2 mol of ethanol can be produced from 1 mol of glucose.

(Experimental Example 4) Candida shahatae (Ca
Ndida Shehatae) was tested for ethanol tolerance. In the experiment, four 300 mL flasks were prepared and No. 1 and No. In the second flask, add 100 mL of 5.0% ethanol-added potato dextrose liquid medium.
Put No. 3 and No. 100 mL of only the potato dextrose liquid medium was placed in the flask of No. 4. No. 1
And No. Candida Shahatae (Ca
ndida Shehatae) HYO-503 strain.
0 × 10 ³ / mL inoculation, No. 2 and No. The parent strain was inoculated into the flask of No. ³ at 4.6 × 10 ³ / mL, and each test was performed by static culture at a culture temperature of 30 ° C. The number of bacteria in each flask was observed for 16 days after inoculation. The number of colonies was calculated by the pour release method using potato dextrose agar. The results are shown in the graph of FIG.

From these results, it was proved that the mutant strain grows in 5.0% ethanol-added potato dextrose liquid medium and grows in the usual medium (potato dextrose liquid medium) in the same manner as the parent strain. That is, as shown in FIG. 9, the parent strain should be killed (live cells are absent) on the 7th day in 5.0% ethanol-containing potato dextrose liquid medium. However, the mutant strain is able to grow in a similar medium (though resistant to 5.0% ethanol), although its logarithmic growth phase is delayed as compared to the growth in a normal medium (liquid medium containing potato dextrose without addition of ethanol). . In addition, the mutant strain showed the same growth ability in normal medium as the parent strain, or a large number of bacteria at the peak. Therefore, it is presumed that this bacterium has a high productivity of ethanol, and it is proved that the productivity is good.

(Experimental Example 5) Next, an experimental example will be described in which acetic acid is produced from apple dregs through an ethanol production process using a mutant strain. Jar fanmenter device (Tokyo Rika Kikai Co., Ltd.) 400g apple cake
To the above, 800 mL of water was added, and 1040 mg of Cellulase A “Amano” 3 (Amano Enzyme Inc.) was added to give 40
After stirring at C for 24 hours, the mutant strain was inoculated at 1 × 10 ⁵ / mL. The ethanol fermentation temperature was 30 ° C. Next, for acetic acid fermentation, inoculum of acetic acid bacteria is 1 × 10 ⁶ / mL
And the mutant strain was inoculated 13 days after the inoculation. The amount of acetic acid was measured by an enzymatic method (F kit acetic acid Roche Diagnostics). The acetic acid fermentation was always aerated and the temperature was 25 ° C. The results are shown in FIG.

If acetic acid bacteria were inoculated for efficient acetic acid fermentation on the 13th day, the highest acetic acid production was obtained. That is, the time when a small amount of glucose still remains promotes the growth of acetic acid bacteria and is a good time for inoculation.

[0047]

As described above, according to the antifreezing agent production system of the present invention, it becomes possible to produce an antifreezing agent from wastes of shells and fruits and vegetables as raw materials, and the effectiveness of the wastes is improved. Can be used. When the calcium producing device is provided with a crusher for crushing the shell washed by the washing machine to make powdery particles of calcium carbonate, calcium carbonate can be easily supplied. Further, when the calcium production apparatus is provided with a calcining machine for calcining the powdery particles of calcium carbonate into powdery particles of calcium oxide, the calcium oxide can be easily supplied.

If the acetic acid production apparatus is provided with an ethanol production section for producing ethanol from waste of fruits and vegetables and an acetic acid production section for producing acetic acid from the ethanol, waste of fruits and vegetables is produced. Since ethanol fermentation is carried out and then acetic acid fermentation is carried out, the steps are separated, and therefore the fermentation efficiency can be improved.
In this case, the ethanol production part is used as a raw material for Candida
When an ethanol fermenter for inoculating a mutant strain of Candida Shehatae and fermenting the raw material while stirring at a predetermined temperature is provided, the fruit and vegetables having a relatively small fermentation source such as glucose such as squeezed lees of apples It effectively works on the waste of, and accordingly, the production efficiency can be improved.

Further, in the acetic acid production part, the acetic acid fermentation tank in which the acetic acid bacterium is inoculated into the raw material and fermented the raw material while stirring at a predetermined temperature, and the raw material which has been fermented in the acetic acid fermentation tank are centrifuged to extract acetic acid. In the case where it is configured to include a centrifuge and an acetic acid storage tank that stores the acetic acid separated by the centrifuge,
Acetic acid with high purity and high concentration can be supplied to the calcium acetate production apparatus from the acetic acid storage tank, and the production efficiency can be improved.

Further, when the calcium acetate producing apparatus is provided with a mixing mixer for spraying acetic acid on the supplied calcium carbonate and / or calcium oxide and stirring the mixture while heating, the mixing should be carried out smoothly. It is possible to improve the production efficiency of calcium acetate. Furthermore, when the feed production apparatus for producing feed from the residue produced when acetic acid is produced by the acetic acid production apparatus is provided, the residue is also used, so that waste can be effectively used without waste. Further, when apple squeezed lees are used as the fruits and vegetables waste and scallop shells are used as the shells, these wastes can be effectively used.

[Brief description of drawings]

FIG. 1 is an overall view showing a system for producing an antifreezing agent according to an embodiment of the present invention.

FIG. 2 is a diagram showing a configuration of a calcium producing apparatus in the antifreezing agent producing system according to the embodiment of the present invention.

FIG. 3 is a diagram showing a partial configuration of an ethanol production unit and an acetic acid production unit of an acetic acid production apparatus in the antifreezing agent production system according to the embodiment of the present invention.

FIG. 4 is a diagram showing a partial configuration of an acetic acid production unit of the acetic acid production apparatus in the antifreezing agent production system according to the embodiment of the present invention.

FIG. 5 is a diagram showing a configuration of a calcium acetate production apparatus in the antifreezing agent production system according to the embodiment of the present invention.

FIG. 6 is a view showing a configuration of a feed producing apparatus in the antifreezing agent producing system according to the embodiment of the present invention.

FIG. 7 is a table showing the assimilation ability of a mutant strain and a parent strain of Candida shahatae according to an experimental example of the present invention.

FIG. 8 is a table showing, in comparison with the experimental example of the present invention, the growth of the strains of the mutant strain and the parent strain of Candida shachatae and the amounts of glucose and ethanol produced.

FIG. 9 is a graph showing the results of an ethanol tolerance test of a mutant strain of Candida shahatae and a parent strain according to the experimental example of the present invention.

FIG. 10 is a graph showing a time-dependent change in the amount of acetic acid produced from squeezed lees of apples according to the experimental example of the present invention.

[Explanation of symbols]

1 Calcium production equipment 2 carrier 3 washing machine 4 conveyor 5 crusher 6 conveyor 7 baking machine 8a cyclone 8b exhaust fan 9 carrier 10 Acetic acid production equipment 11 Ethanol generator 12 Ethanol fermenter 13 hoppers 14 Stirrer 15 Temperature controller 16 pumps 20 Acetic acid generator 21 Acetic acid fermenter 22 Air supply machine 23 Stirrer 24 Temperature controller 25 gas venting machine 26 pumps 30 centrifuge 31 Acetic acid storage tank 32 stirrer 33 Temperature controller 34 pumps 35 Regulator 40 Calcium acetate manufacturing equipment 41 mixing mixer 42 hopper 43 Temperature controller 44 Cyclone 45 Exhaust fan 50 Feed production equipment 51 dryer 52 Conveyor 53 Burner device 54 conveyor 55 cyclone 56 Exhaust fan

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Norimitsu Hanamatsu             Aomori Prefecture Aomori City 2nd wholesale town 4-11-6             Aomori Industrial Technology Development Center (72) Inventor Masago Kushibiki             Aomori Prefecture Aomori City 2nd wholesale town 4-11-6             Aomori Industrial Technology Development Center (72) Inventor Shinya Yamaguchi             Aomori Prefecture Aomori City 2nd wholesale town 4-11-6             Aomori Industrial Technology Development Center (72) Inventor Hideo Odagiri             85 Maeda, Tateyama, Hiraga-cho, Minamitsugaru-gun, Aomori Prefecture             Ground 2 Joy World Pacific Co., Ltd.             In the wick (72) Inventor Mayumi Yamabata             85 Maeda, Tateyama, Hiraga-cho, Minamitsugaru-gun, Aomori Prefecture             Ground 2 Joy World Pacific Co., Ltd.             In the wick (72) Inventor Yoshiharu Kim             2-23-3 Tsurumaki, Tama-shi, Tokyo Co., Ltd.             Inside the New Land Development Institute F-term (reference) 4D004 AA02 AA16 BA04 BA06 CA04                       CA18 CA20 CA30 CC07                 4H020 AA01 AA03 AA04 AB01

Claims (10)

[Claims] 1. A system for producing an antifreezing agent for producing calcium acetate as an antifreezing agent, comprising: a calcium producing apparatus for producing calcium carbonate and / or calcium oxide using shells as a raw material; and acetic acid using wastes of fruits and vegetables as a raw material. Acetic acid production apparatus for producing Calcium and calcium carbonate and / or produced by the calcium production apparatus
Alternatively, a system for producing an antifreezing agent, comprising: a calcium acetate production apparatus for producing calcium acetate by mixing calcium oxide and acetic acid produced by the acetic acid production apparatus while heating. 2. The calcium producing apparatus comprises a washing machine for washing shells, and a mill for crushing the washed shells into calcium carbonate powder and granules. Item 1. A system for producing an antifreezing agent according to Item 1. 3. The calcium producing apparatus is provided with a calciner for calcining the calcium carbonate powder and granules to form calcium oxide powder and granules.
The antifreeze production system described. 4. The acetic acid production apparatus comprises an ethanol production section for producing ethanol from waste of fruits and vegetables and an acetic acid production section for producing acetic acid from ethanol produced by the ethanol production section. The manufacturing system of the antifreezing agent according to claim 1, 2, or 3. 5. The ethanol producing unit is used as a raw material for Candida Shehatae.
5. The system for producing an antifreezing agent according to claim 4, further comprising an ethanol fermenter for inoculating the mutant strain of claim 1 and fermenting the raw material while stirring at a predetermined temperature. 6. An acetic acid fermenter for inoculating an acetic acid bacterium into a raw material and fermenting the raw material while stirring the raw material in the acetic acid producing section, and centrifuging the raw material that has been fermented in the acetic acid fermenter. The antifreezing agent manufacturing system according to claim 4 or 5, comprising a centrifuge for extracting acetic acid and an acetic acid storage tank for storing the acetic acid separated by the centrifuge. 7. The calcium acetate production apparatus is provided with a mixing mixer for spraying acetic acid on the supplied calcium carbonate and / or calcium oxide and stirring the mixture while heating. A system for producing an antifreezing agent according to 2, 3, 4, 5 or 6. 8. A feed producing apparatus for producing feed from a residue produced when acetic acid is produced by the acetic acid producing apparatus is provided in any one of claims 1, 2, 3, 4, 5, 6 or 7.
The antifreeze production system described. 9. The squeezed lees of apples are used as the waste of fruits and vegetables, 1, 2, 3, 4, 5, 5.
A system for producing an antifreezing agent according to 6, 7, or 8. 10. The scallop shell is used as the shell, 1, 2, 3, 4, 5, 6, 7,
8. The system for producing an antifreezing agent according to 8 or 9.