JP2003321384A - Concentrated extract of sasa albo-marginata and its manufacturing method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide concentrated extracts of Sasa albo-marginata having a low viscosity though containing a high concentration of effective ingredients from Sasa albo-marginata, and a method for efficiently manufacturing the same. <P>SOLUTION: The concentrated extracts of Sasa albo-marginata are prepared by concentrating extracts, obtained by subjecting Sasa albo-marginata to extraction with hot water plural times, cooling and liquid-solid separation. The manufacturing method thereof is also provided. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a concentrated extract from Kumazasa and a method for producing the same. More specifically, the present invention relates to a concentrated extract of Kumazasa, which has a high concentration of active ingredient and low viscosity, and a method for efficiently producing the same.

[0002]

2. Description of the Related Art Kumazasa is a genus of the grass family Sasa kuril.
ensis) is a large-sized rice, rice cake, chimaki,
It has been used as a material for food packaging since ancient times, as is known for Sasa sushi. This application uses the antiseptic effect of Kumazasa that wrapping cooked rice and rice cake with leaves of Kumazasa increases the shelf life, but recently, the effects of Kumazasa such as bioregulatory function have been proved, and It has been reported that the polysaccharide component in Kumazasa has antitumor activity and that its effect was confirmed in animal experiments. For these reasons, in recent years, for the purpose of maintaining health, a concentrated extract of Kumazasa containing Kumazasa polysaccharide and the like has been taken by dissolving it in water, hot water or the like.

In order to obtain the concentrated extract of Kumazasa, generally, a method of putting dried Kumazasa in a clay pot or the like, heating it on a low heat and then allowing it to stand, collecting only the supernatant liquid and concentrating it is used. It was being done. However, in such a method, in order to obtain a pure supernatant liquid, a great amount of time is required as a standing time after heating, which is extremely inefficient for industrial production. On the other hand, if the standing time was short and insufficient, impurities were mixed in the supernatant, and a highly pure concentrated extract could not be obtained.

Further, among the conventional concentrated extracts, those of high concentration in which the solid content is concentrated to 50% or more often contain impurities. In this case, since the viscosity of the concentrated extract becomes high, the fluidity of the liquid is deteriorated, and clogging occurs at the dropping port of the container at the time of use, resulting in poor usability. Further, when the concentrated extract has a high viscosity, it has a problem that it is difficult to dissolve in water or hot water when taken.

[0005]

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned circumstances, and by reducing impurities such as contaminants and precipitates, it is possible to efficiently obtain a concentrated extract of Kumazasa, which has a high concentration but a low viscosity. It is an object of the present invention to provide a method for producing the same.

[0006]

Means for Solving the Problems The inventors of the present invention have conducted extensive studies to solve the above problems, and as a result, concentrated the extract obtained by extracting Kumazasa with hot water several times.
It was found that by cooling in a stationary state to remove impurities such as impurities and precipitates, a concentrated extract of Kumazasa, which has a high concentration and a low viscosity, can be efficiently produced. Further, it was found that the concentrated extract of Kumazasa obtained by such a method has high purity and thus can be stored without using a preservative or the like, and thus completed the present invention.

That is, the present invention provides a concentrated extract of Kumazasa obtained by concentrating the extract obtained by extracting Kumazasa with hot water a plurality of times, further cooling and solid-liquid separation.

The present invention also provides a concentrated extract of Kumazasa which comprises a first step of hot water extraction of Kumazasa a plurality of times, and a second step of concentrating the extract obtained in the first step and further cooling and solid-liquid separation. The present invention provides a method for manufacturing the same.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, Kumazasa, which is a raw material for obtaining a concentrated extract, is a plant belonging to the genus Sasa kurilensis (Sasa kurilensis), which is also called kumai sasa or chishimasa. This Kumazasa may be used as it is collected, but if necessary, it is preferably dried and finely chopped or crushed in advance so that the concentrated extract can be easily extracted.

This Kumazasa is extracted with hot water as the first step. This hot water extraction can be performed, for example, by putting Kumazasa and water in an extraction pot and heating the extraction pot. The higher the heating temperature is, the easier the solid content is extracted, but it is generally preferable to carry out the heating at a temperature of about 80 to 90 ° C., and the heating time is 1 time per extraction.
It is preferable to carry out for about 2 hours. This hot water extraction need not be performed under pressure, but may be performed under pressure if necessary.

The amount of water used as a solvent in this hot water extraction is not particularly limited, but an amount that allows Kumazasa to be completely immersed may be appropriately used. Usually, it is at least 10 times by weight the amount of Kumazasa as a raw material, and preferably. It is sufficient to use 40 times the amount of water or more.

In the production method of the present invention, this hot water extraction is repeated a plurality of times. By repeatedly performing hot water extraction in this manner, the active ingredient can be almost completely extracted from the Kumazasa raw material, which is generally considered to be difficult to extract.

In order to obtain an extract by hot water extraction, first, the Kumazasa raw material is hot water extracted by the above-mentioned procedure, and the extract is collected. Preferably, the procedure of adding 40 times or more water and performing hot water extraction similarly may be repeated. Generally, the hot water extraction of the Kumazasa raw material is preferably performed three times or more, and the solid content in the Kumazasa can be almost completely extracted by performing such heat extraction.

The extract obtained in the above first step is
Then, in the second step, the solution is concentrated, further cooled, and then solid-liquid separated.

For concentration of the extract, various concentration methods such as a reverse osmosis membrane concentration method can be used, but it is preferable to use a vacuum concentration method. Regarding the conditions for concentration, a conventional method may be used. Further, in this concentration, in consideration of the fact that the solid concentration is reduced by 2 to 3% due to the removal of impurities in the solid content in the subsequent steps, the concentrated liquid is set to a state of about 2 to 3% higher than the final target solid concentration. It is preferable to set.

The concentrated Kumazasa extract is sterilized by heating if necessary, and then cooled under a temperature condition of 0 to 10 ° C. At the time of this cooling, it is desirable that the extract is allowed to stand, so that impurities called so-called sediment can be effectively precipitated. The time for cooling the extract is a time sufficient for the impurities in the extract to be insolubilized and precipitated, but specifically, it may be appropriately determined depending on the amount of the extract and the required purity, for example, It may be carried out for 1 day or longer, preferably 3 days or longer.

Examples of solid-liquid separation means for removing precipitated impurities from the cooled extract include filtration and centrifugation. Among them, as the filtration, filtration using a diatomaceous earth precoat filter press, a filter press such as diatomaceous earth precoat suction filtration, or suction filtration is preferable. In addition, the centrifugal separation is performed using a commercially available centrifugal separator (for example, Self-Jector: manufactured by Mitsubishi Kakoki Co., Ltd.) at a rotation speed of about 5000 to 15000 rpm, and the supernatant after the centrifugation is performed. However, it is preferable to add water to the remaining sedimentation portion, centrifuge again, and collect the supernatant again, because the concentrated extract can be collected completely.

The second step may be performed once, but it is preferably repeated a plurality of times. In this case, the cooling time and the solid-liquid separation means can be appropriately selected according to the amount of impurities expected to be precipitated. For example, when the second step is repeated twice, the initial cooling time is shortened to, for example, about 2 days, the precipitated impurities are removed by a filter press, and the second cooling time is set to 1
It is preferable to make the length as long as about a week and remove the precipitated impurities by centrifugation or the like. By doing so, most of the impurities having a relatively large molecular weight can be removed in the first step, and even impurities of a relatively small molecular weight can be completely removed in the subsequent step. It is effective in further increasing the purity of the components in the Kumazasa concentrated extract.

The extract from which impurities have been removed by the solid-liquid separation treatment in the second step is optionally sterilized by heating, and preferably 3 to 1 at 5 to 20 ° C. for decantation.
It is left for about 0 days. After leaving, if you collect the supernatant,
The target Kumazasa concentrated extract is obtained.

The concentrated extract of Kumazasa obtained by the above method has a small amount of impurities such as contaminants and precipitates, and thus has a high solid concentration of 50% or more, but a low viscosity of 100 cp or less. It is a thing. Therefore,
The fluidity of the liquid is good, and there is no problem such as clogging at the dropping port even when used by being filled in the container,
It has excellent usability. Furthermore, since it has a low viscosity, it is easily dissolved in water, hot water, etc. even when it is taken.

The concentrated extract of Kumazasa has a high concentration and a high solid content, and therefore has excellent preservability without adding any preservative such as benzoate or ethanol. .

[0022]

EXAMPLES Next, the present invention will be described in more detail by way of examples, which should not be construed as limiting the invention thereto.

Example 1 Production of concentrated extract of Kumazasa: dried Kumazasa (Sasa v
eitchii) After shredding 1000 kg, 20000 kg
It was put in a 30,000 liter extraction pot with water (20 times the amount of Kumazasa). Next, the extraction vessel was heated to 90 ° C. and hot water extraction was performed for 1 hour to recover the extract.

After collecting the extract, 20,000 kg of water was added again to the residue in the extraction kettle, and the mixture was heated to 90 ° C. and subjected to hot water extraction for 1 hour to collect the extract. This extraction operation was performed once more with the extraction time of 30 minutes (total 3 times) to obtain 57 kl of Kumazasa extract.

Extraction liquid 57 obtained by the above three hot water extractions
kl is filtered using a mesh screen with a mesh number of 100 to remove impurities, and then the liquid temperature is 55 to 65 ° C.
The solution was concentrated under reduced pressure under the conditions of, and the extract was concentrated to 2000 kg. After sterilizing this concentrated solution by heating, (condition: 90 ° C x 1
0 seconds), and left to cool in a refrigerator (temperature: 5 ° C.) for 2 days.

The statically cooled concentrated liquid was filtered using a diatomaceous earth precoat filter press to remove impurities, and then the filtrate was concentrated again under reduced pressure at a liquid temperature of 60 to 65 ° C. to give a solid concentration of 55%. It was concentrated until. The filtrate was allowed to stand and cool for 1 week in a refrigerator (temperature: 5 ° C).

The still-cooled concentrated solution was subjected to centrifugal separation by discharging every 5 minutes at a rotation speed of 7,000 rpm using a centrifuge (self-jector: manufactured by Mitsubishi Kakoki Co., Ltd.). After collecting the supernatant, the residue was watered and centrifuged again under the same conditions to collect the supernatant. Heat sterilization of the supernatant collected by centrifugation twice (condition 90 ° C x 1
After 0 minutes), the mixture was refrigerated and stored in a refrigerator at 5 ° C. for 1 week, and then the precipitate was removed to obtain 80 kg of Kumazasa concentrated extract (Product 1 of the present invention).

The content and viscosity of the solid content of the product 1 of the present invention obtained by the above-mentioned production method were measured using a B-type viscous mold. As a control, a commercial product was also measured (Comparative products 1 and 2). The results are shown in Table 1.

(Result)

[Table 1]

From the results shown in Table 1, the Kumazasa concentrated extract of Product 1 of the present invention had a low solid content of 50 cP and a low viscosity of 80 cP.

Example 2 Storage test of concentrated extract of Kumazasa (1): 35 g of concentrated extract of Kumazasa of the present invention 1 obtained in Example 1 was used for 30 m.
A test sample was put in a 1-liter bottle and capped. These four test samples were placed in an environment of 1 to 30 ° C., and the operation of opening each of the test samples twice a day for 5 minutes each in the morning and 5 minutes each, leaving them for a while, and then plugging them was repeated. With respect to this sample, the number of bacteria (mold and yeast) in the Kumazasa concentrated extract was measured every two weeks using a polydextrose agar medium to confirm the storability of the sample. The measurement results are shown in Table 2.

(Result)

[Table 2]

From the results shown in Table 2, it was confirmed that the presence of bacteria was not recognized even after 6 weeks from the start of the test, the quality at the time of production was maintained, and that the Kumazasa concentrated extract of the present invention had good preservability. did it.

Example 3 Storage test of concentrated extract of Kumazasa (2): Bread yeast and Bacillus natto were inoculated into the extract of Kumazasa of the product 1 of the present invention, and the change in the number of bacteria was confirmed over time. The results are shown in Table 3.

(Result)

[Table 3]

From the results shown in Table 3, it was confirmed that the Kumazasa concentrated extract of the present invention suppressed the growth of bacteria even after 2 weeks.

[0037]

EFFECTS OF THE INVENTION The method of the present invention is to extract the solids in the raw material of Kumazasa almost completely by performing hot water extraction of Kumazasa a plurality of times. Further, the extract is concentrated, cooled and filtered. Therefore, impurities such as contaminants and precipitates can be deposited quickly, and the concentrated extract of Kumazasa containing few impurities can be obtained very efficiently, without requiring a long standing time after heating as in the conventional case. Is something that can be done.

Further, this concentrated extract of Kumazasa has a high concentration but a low viscosity, so that the fluidity of the liquid is good, and even when it is used by being enclosed in a container, it is clogged at the dropping port. There is no problem such as the above, and it is excellent in usability. Furthermore, since it has a low viscosity, it is easily dissolved in water, hot water, etc. even when it is taken.

Furthermore, since the concentrated extract of Kumazasa obtained by the method of the present invention has a high concentration and a high purity, it has excellent preservability without adding any preservative such as benzoate or ethanol. It has high commercial value and can be taken stably for a long period of time. that's all

Claims (5)

[Claims] 1. A concentrated extract of Kumazasa, which is obtained by concentrating an extract obtained by subjecting Kumazasa to hot water extraction a plurality of times, further cooling and solid-liquid separation. 2. The concentrated extract of Kumazasa according to claim 1, which has a solid content concentration of 50% by mass or more and a viscosity of 100 cP or less. 3. A method for producing a concentrated extract of Kumazasa, which comprises a first step of extracting Kumazasa with hot water a plurality of times, and a second step of concentrating the extract obtained in the first step and further cooling and solid-liquid separation. 4. The method for producing a concentrated extract of Kumazasa according to claim 3, wherein the cooling in the second step is performed for at least one day or more. 5. The method for producing a concentrated extract of Kumazasa according to claim 3 or 4, wherein the second step is repeated a plurality of times.